CmoCh16G010840 (gene) Cucurbita moschata (Rifu)

NameCmoCh16G010840
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionSterol C4-methyl oxidase 1-2
LocationCmo_Chr16 : 7665012 .. 7669681 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCCTTTCCACTCCCTCTCCGCCGCCCAGCTTGCGCTTGGCCGCAATCTCACCTTCTTCGAGACCCTCTGGTTCAACTACTCCCACGACAAGTCCGATTACTTCCTCTTTTGCCATAACATTCTCTTCCTGTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTTATCTTTCTCGAGATCTTGCACGCCTCCGGGATTCACAAGTACAAAATTCAGCCCAAAGTCCGGTTGCCATTTTCCGAGATCTTCCGCTGCTACAAGGATGTTATGCGCATGTTCTTCCTTGTTGTTGGTCCTCTTCAGCTCGTCTCATTCCCTTCCATTCAGGTGAATCTCGGGTTTCGATTTCGCGATTTCTACCCTCTTGTTGATTTAGGATTTTGATTTTGAGATTGTTTTTCGGTTTCTCTTGGATCATTAGATGATTGGAATCAGGACGGGTTTGCCATTGCCTTCTGGTTGGGAAATTGTTTCTCAATTGGTGGTGTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTCCTCCATTGCAAATGGGGGTATGAGAATATCCATCAAGTTCATCATGAATACACTGCTCCTATTGGATTTGCAGCTCCTTACGCTCATTGGGCGGAGGTTTTGATCCTTGGAATCCCCTCTTTTCTTGGTCCTGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTGTGGATTGCGCTAAGGCAGATTGAGGCCATTGACACGCATAGTGGGTATGAATTCTAAAGCTATATCTCTCTCACACTGTGTTAAAAGTACTTTTAATCTGAAGGATCTTGAATGGTGGGCTTACCTCTTTTTTGACTACGACCGAGATCTGCTCTATTGCTTTCTTCTCTTTTAATCTTGATTTCCTTTTCCTCATTATATATATATATATATATATATATTTTATCTATATGGAGTTCCATCATTACTGTATGAATTTATTGCATGCTGAATGATTGATCCTACTAACTTGATATAACGAAGTTTGAAGTTTTGTGTTGAGAAATATTGAAAGGGTTAAATTTACCCTTCTTTGAGTTGTCTAGCATCAACTTTTTCAGCTTCCACATTCTTCATACAGCTGTGTGAAAGCTCGATATTCCTTTGAATTTATCGTAAGGGGGTGTATATATCTTCTATGAGAAGCTTAGCTATGAAGCTTGATTCTTGTATACTAAACTTATTATAGGGCCCACTACCCCTTTATGCTTTAAGCTTATGGATATCATTTCAAGATCCATGATTTAACATGCTATGAAGTAGCTAGTATTGAGTATTGTTATTTTTTTCTTCTCAATTTTGTATTAGGCTTTGTGTGCTAACATTTGACTTTACTTTCTCTGATTAGTGGGACCGTAGGTGTATTAGACTATAGTTTTTAGTGAAGAATGAGATTCGTGTATGAGGAAAGGAAAAACAACGACGTGGTTGTGTCAATGTGTGATGATATCGGTGGGCAAAGATCATGAACATGATTATAACCGAACCTTCTTGGTTCAAACATTCTTCACTTCAGGGGAGCCTTTAGCTCACGATCATTCCCGATCTTCTGTATCTTACTCCATTGTAGTTGTTTTTGGGGCTTCACCTTTTCGATCTCATTGTTAAGATTTCATAACCTTGGGATTGTTGGGAAATGAAAGGAAGTTCGGAGCTGTTGTGTAGAAAGAAATCTTTAGAGAATTGCTTCTAGCCCGATGGAGTAGACATATAGGGTGCTCACGGGTTAGACTCGTGTAACTTGGGTTAAGAAGCCTAACATATTCTAGCATACGGTTTGCTTATTTTGATCTACGAGTTTGTTAATTCCCTGGGTTACATAAACCCACTAGTTATACGAATCCAAGTTTCCATAACTGTGAGATCCCACCTCGAGAGGGGAACGAAGCATTCTTTATAAGGGTGTGGAAACCTTTCCCCAGCAGACAGTGTTTTGAAAACTTTGAAGGGAAACCCGACGGGAAAGCCCATCTGCTAGCGGTGGGCTTGGGCTGTTACAATAATCTACCACTCAGTACGTTCTGAACACAAATAAATTATATAAACTCAGCTTTTCAATAAACCAACCTTATTCAATTGTATTGTTCCTCTTCCTCCTCAGAAGAATCGAGAAGCTCTGTTATTCATGTTGGCTCAATCGTTTTAGAGGAGTGTAGAGGTAACAAAAGTAAATTGACATCTCAAAGGGCCAGCTTACTCAAATAAGAATCCATTGATTTGACCTCCAAATTGTTTAGTTGGTCAGAAACGAAAAATAATTGATGTCAGGCTTATGGCTGGGAACAAATGCGGTGTCAGACAATCATTTCTTTGGTCATGTAGGACATAGCTTCACTCTTGGTGGGTTTCCTGTGGCTGAACATCCCTTTAGCCCTTGGCTGAACTCGAATAACTTTATGTGTTATTATTTTATATCCCTGAAGTTTAATTGTTTTGTCTCTTTCACAGAAATATTGAGATTCGTATGCACATTGATGGAAAGTTGTTGTATAACTTTTGGTGCTAACGAGAAAGTCGAAAAAATTTTAAAAATGAAGAAACTCACATCAATAATCTGAAGTTGAACTATTGACTTCTTTGGACTTTCCCTTTGTATTATGTTTTCTATCGATGTGAATGAGAGGAATGATGGTTTAATGGGTAAGCCTTACTGCATATCTCCAAAATCTACTGTTTTCTCTCTTTACCATGTCGACTTAAACTTACTACTTGTGAGATCCCACCTCGATTGGAGAGGAAAATGAAACATTTTTTACAAGGGTGTGGAAACCTTTTCCAGTAGACGCATTCTAAAACCATGAGGGGATATCCATAAGGGAAATCCAAAAAGGACAATATCTGCTAGCGGTGGGCTTGAGTTGTTACACTACTAGAGATGTTATGGGAGGTATACCAAACTAGAGGGACTATAATAAACTTTTAAAACTATAGAGATCAAGTTGAAGTCAAGTGCAAACCATAGAGACTAAAATCGTAATACAATCATACTTTGAGATCTCACATCGATTGGAGAGAACAAGTGCATTCTTTATAAGGGTGTGAAAACTTCTCTCTAGCAGACGCGTTTTAAAAACCTTGAGGGAGGGAAAATCCCAAAGAAGATAATATCTACTCGCGGTCGCTGGGCGTCAAAGGGGATGGATTGTAAGATTCCACATCGATTGGAGAGAAGAATGATTGCATTCTTTATAAGGGTGTGAAAATCTTTCTTAGAAGACATGGTTGGAAAGCCTAAAGAGGACAAAATACATCATTTATCTTCCTTTTAGTGTGTTCTTTTTCTTTTCCTCCTCTGCTTTTCTCTGAGATTGTGCTTTTATGATGGCCTGTTGAGATCATCCCTGGTTTTATTCATACATATTATATTCATATTCAGGTATGATTTCCCCTGGAGTCTAACGAAATTCATACCATTTTACGGTGGTTCGGACCATCATGATTACCATCACTATGTTGGAGGACAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGTAGTTATGCTGCTCCTCTTATAATTTTACCTCCCACTTGTTCTTCCAGTTAAACCATGCTCATAAAAGTTACTCGTTTGTTTACTTCCTCAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGGTAAGCATTTTGTTTTCGCCTCCGCCCCCACCTTCACCCCGAAAATTGATTGTATATGATGTCGTGAGCTTGCATTGATTTCATGTTTTCTCCAACTGGACATTAGATATACTCATCTATGAGCTTGCTTCGATTGCGTGGCCTTGGGATTGCTAGTTTAACTATATTTGGTTGGATCAAATGTGAGATCCCACATTGGTTGGAGAGGGGAACGGAGCATTCCTTATTGGGTGTGGAAACCTCTCCCTAACAGACGTGTTTTAAAACCGTGATGCTGACAGCGATACGTAGCAGGCTAAAATGGACAATATTTGTTAGCGGTGGGTTTGGGTTGTTACAAATGGTATTAAAGCCAGACACCGGGCGGTGTCCCAGTGATGTCGCTGGGTCCCTAAGGGGGTAGATTGTGAGATCTCACGTCGGTTGGAGAGGGGAACAAAGCATTCCTCATAGGATGTGGAAACCTCTCCCTAACAGACACATTTGATAACCATGAGACTAACGGGATACGCAAGTGGCTAAAACGGACAATATCTACTAACGGTGGGCAGCTTGGGCTGTTACATCAAATCTATTTCCTTTGTTCTTTTTAACCGGCTACCCCGTACCTGTTAAATCATTGTGTGTTCTAATTGTTTGTTGGTGGTGGAATCAGTTGAAGGAAGAAGTGAAAAGCAGTGAAGGATCATACTACAGCACAGCCGAAGATGTTAAAGTGGACTAGACGATGAATCGGGGAACGAGGGATATTCATGTTTTGAAGGATGGGGGGTTTTTTTTCGTGTCGGTAGCAATGTTACGTGGTCTCATCGACGAGGTAGTTAGGGAGTGGGATGGGAAGGAAATTAACATGGGGTCTATAAAAAAAGTTTAATTTTTGTTAGCTTAATATGTCGAGGCAGAGAAGTCATATTCTCCCGTGGCTATGTGTGTGGGTTTAACCTTATTGTGCAGACAAAACTGGTAATATAAATATGTATTCTGGTTGTGAAATTTAA

mRNA sequence

ATGTTGCCTTTCCACTCCCTCTCCGCCGCCCAGCTTGCGCTTGGCCGCAATCTCACCTTCTTCGAGACCCTCTGGTTCAACTACTCCCACGACAAGTCCGATTACTTCCTCTTTTGCCATAACATTCTCTTCCTGTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTTATCTTTCTCGAGATCTTGCACGCCTCCGGGATTCACAAGTACAAAATTCAGCCCAAAGTCCGGTTGCCATTTTCCGAGATCTTCCGCTGCTACAAGGATGTTATGCGCATGTTCTTCCTTGTTGTTGGTCCTCTTCAGCTCGTCTCATTCCCTTCCATTCAGGTGAATCTCGGGTTTCGATTTCGCGATTTCTACCCTCTTATGATTGGAATCAGGACGGGTTTGCCATTGCCTTCTGGTTGGGAAATTGTTTCTCAATTGGTGGTGTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTCCTCCATTGCAAATGGGGGTATGAGAATATCCATCAAGTTCATCATGAATACACTGCTCCTATTGGATTTGCAGCTCCTTACGCTCATTGGGCGGAGGTTTTGATCCTTGGAATCCCCTCTTTTCTTGGTCCTGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTGTGGATTGCGCTAAGGCAGATTGAGGCCATTGACACGCATAGTGGGTATGATTTCCCCTGGAGTCTAACGAAATTCATACCATTTTACGGTGGTTCGGACCATCATGATTACCATCACTATGTTGGAGGACAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGGTAAGCATTTTGTTTTCGCCTCCGCCCCCACCTTCACCCCGAAAATTGATTTTGAAGGAAGAAGTGAAAAGCAGTGAAGGATCATACTACAGCACAGCCGAAGATGTTAAAGTGGACTAGACGATGAATCGGGGAACGAGGGATATTCATGTTTTGAAGGATGGGGGGTTTTTTTTCGTGTCGGTAGCAATGTTACGTGGTCTCATCGACGAGGTAGTTAGGGAGTGGGATGGGAAGGAAATTAACATGGGGTCTATAAAAAAAGTTTAATTTTTGTTAGCTTAATATGTCGAGGCAGAGAAGTCATATTCTCCCGTGGCTATGTGTGTGGGTTTAACCTTATTGTGCAGACAAAACTGGTAATATAAATATGTATTCTGGTTGTGAAATTTAA

Coding sequence (CDS)

ATGTTGCCTTTCCACTCCCTCTCCGCCGCCCAGCTTGCGCTTGGCCGCAATCTCACCTTCTTCGAGACCCTCTGGTTCAACTACTCCCACGACAAGTCCGATTACTTCCTCTTTTGCCATAACATTCTCTTCCTGTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTTATCTTTCTCGAGATCTTGCACGCCTCCGGGATTCACAAGTACAAAATTCAGCCCAAAGTCCGGTTGCCATTTTCCGAGATCTTCCGCTGCTACAAGGATGTTATGCGCATGTTCTTCCTTGTTGTTGGTCCTCTTCAGCTCGTCTCATTCCCTTCCATTCAGGTGAATCTCGGGTTTCGATTTCGCGATTTCTACCCTCTTATGATTGGAATCAGGACGGGTTTGCCATTGCCTTCTGGTTGGGAAATTGTTTCTCAATTGGTGGTGTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTCCTCCATTGCAAATGGGGGTATGAGAATATCCATCAAGTTCATCATGAATACACTGCTCCTATTGGATTTGCAGCTCCTTACGCTCATTGGGCGGAGGTTTTGATCCTTGGAATCCCCTCTTTTCTTGGTCCTGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTGTGGATTGCGCTAAGGCAGATTGAGGCCATTGACACGCATAGTGGGTATGATTTCCCCTGGAGTCTAACGAAATTCATACCATTTTACGGTGGTTCGGACCATCATGATTACCATCACTATGTTGGAGGACAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGGTAAGCATTTTGTTTTCGCCTCCGCCCCCACCTTCACCCCGAAAATTGATTTTGAAGGAAGAAGTGAAAAGCAGTGAAGGATCATACTACAGCACAGCCGAAGATGTTAAAGTGGACTAG
BLAST of CmoCh16G010840 vs. Swiss-Prot
Match: SMO12_ARATH (Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana GN=SMO1-2 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 1.8e-128
Identity = 212/294 (72.11%), Postives = 252/294 (85.71%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +AL RNLT+ ETLWF+YS  KSDY+L+CHNILFLFLIFS +PLPL+F+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  ILHASG--IHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFR 120
              ++    ++YKIQPKV+  FS +F+CYKDVM+MF LVVGPLQLVS+PSIQ        
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQ-------- 120

Query: 121 DFYPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYT 180
                MI IR+GLPLPS  EIV+QLVVYF+VEDYTNYW+HRF HCKWGYE  H +HHEYT
Sbjct: 121 -----MIEIRSGLPLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYT 180

Query: 181 APIGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSL 240
           APIG+AAPYAHWAEVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAI+THSGYDFPWSL
Sbjct: 181 APIGYAAPYAHWAEVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSL 240

Query: 241 TKFIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKV 293
           TK+IPFYGG+++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++
Sbjct: 241 TKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQM 281

BLAST of CmoCh16G010840 vs. Swiss-Prot
Match: SMO11_ARATH (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 1.8e-125
Identity = 208/294 (70.75%), Postives = 253/294 (86.05%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +ALGRNLT  ETLWF+YS  KSDY+L+CHNILFLFL+FS +PLPL+F+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  ILH-ASGI-HKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFR 120
           +   ASG+ ++YKIQPKV    S++F+CYKDVM MF LVVGPLQLVS+PSIQ        
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQ-------- 120

Query: 121 DFYPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYT 180
                MI IR+GLPLP+  E++SQLVVYF++EDYTNYW+HRF H KWGY+ IH+VHHEYT
Sbjct: 121 -----MIEIRSGLPLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYT 180

Query: 181 APIGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSL 240
           APIG+AAPYAHWAEVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAI+THSGYDFPWS 
Sbjct: 181 APIGYAAPYAHWAEVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSP 240

Query: 241 TKFIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKV 293
           TK+IPFYGG+++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++
Sbjct: 241 TKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQI 281

BLAST of CmoCh16G010840 vs. Swiss-Prot
Match: SMO13_ARATH (Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana GN=SMO1-3 PE=2 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 2.7e-116
Identity = 190/289 (65.74%), Postives = 239/289 (82.70%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +ALGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
                   ++KIQ KV+   S++F+CYK+VM++F LVVG LQ+VS+PSIQ          
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQ---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              M+GIR+GLPLPS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYE IH++HHEYT+P
Sbjct: 121 ---MVGIRSGLPLPSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IG+A+PYAHWAE+LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK
Sbjct: 181 IGYASPYAHWAEILILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL 290
            IPFYGG ++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L
Sbjct: 241 LIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLL 274

BLAST of CmoCh16G010840 vs. Swiss-Prot
Match: SMO22_ARATH (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.5e-55
Identity = 113/271 (41.70%), Postives = 156/271 (57.56%), Query Frame = 1

Query: 19  TFFETLWFNYSHDKSDYFLFCHNILFLF-LIFSFIPLPLIFLEILHASGIHKYKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP IFLE      + KYKIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDFYPLMIGIRTGLPLPSGW 138
            P ++  +C   ++   F V  PL L S+P  +              +G+R+  PLPS  
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRA-------------MGMRSSFPLPSWK 122

Query: 139 EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEVLILG 198
           E+ +Q++ YF++ED+  YW HR LH KW Y+N+H VHHEY  P G  + YAH AE+L LG
Sbjct: 123 EVSAQILFYFIIEDFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLG 182

Query: 199 IPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDYHHYV 258
             + +GPA+   H+IT WLW+ LR +E ++ H GY FPWSL+ F+P YGG+D HDYHH +
Sbjct: 183 FATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRL 242

Query: 259 GGQSHSNFASVFTYCDYIYGTDKGYRYQKKI 289
                 N++S F Y D+I+GTDKGYR  K +
Sbjct: 243 LYTKSGNYSSTFVYMDWIFGTDKGYRRLKTL 257

BLAST of CmoCh16G010840 vs. Swiss-Prot
Match: SMO21_ARATH (Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana GN=SMO2-1 PE=2 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 112/270 (41.48%), Postives = 151/270 (55.93%), Query Frame = 1

Query: 33  SDYFLFCHNILFLF-LIFSFIPLPLIFLEILHASGIHKYKIQPKVRLPFSEIFRCYKDVM 92
           SD+ L C     L   +F    LP IFLE      +  YKIQ K   P ++  +C   ++
Sbjct: 17  SDFQLACIGSFILHESVFFLSGLPYIFLE--RTGFLSNYKIQTKSNTPEAQ-GKCIARLL 76

Query: 93  RMFFLVVGPLQLVSFPSIQVNLGFRFRDFYPLMIGIRTGLPLPSGWEIVS-QLVVYFMVE 152
                V  PL + S+P       FRF       +G+ +  PLPS W++VS Q++ YF++E
Sbjct: 77  LYHCCVNLPLMMASYPV------FRF-------MGMESSFPLPS-WKVVSAQILFYFIIE 136

Query: 153 DYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGH 212
           D+  YW HR LH KW Y+N+H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 137 DFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 196

Query: 213 MITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGS------------DHHDYHHYVG 272
           +IT WLW+ LR IE ++ H GY FPWS + F+P YGGS            D HDYHH + 
Sbjct: 197 LITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLL 256

Query: 273 GQSHSNFASVFTYCDYIYGTDKGYRYQKKI 289
                N++S F Y D+I+GTDKGYR  K +
Sbjct: 257 YTKSGNYSSTFVYMDWIFGTDKGYRKLKAL 269

BLAST of CmoCh16G010840 vs. TrEMBL
Match: A0A0A0L5P8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G036480 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 4.2e-161
Identity = 282/330 (85.45%), Postives = 295/330 (89.39%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQ +LGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
           +LHA+GIHKYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+          
Sbjct: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIK---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              MIGIRTGLPLPSG+EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH VHHEYTAP
Sbjct: 121 ---MIGIRTGLPLPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK
Sbjct: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKVSILFSPPP 300
           FIPFYGG+DHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+K         
Sbjct: 241 FIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQK--------- 300

Query: 301 PPSPRKLILKEEVKSSEGSYYSTAEDVKVD 331
                   LKEEVK+SE SYY+TA++VK D
Sbjct: 301 --------LKEEVKNSEESYYNTAQNVKSD 300

BLAST of CmoCh16G010840 vs. TrEMBL
Match: A0A067JRW8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20744 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 3.8e-138
Identity = 241/330 (73.03%), Postives = 274/330 (83.03%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPF++L  A  ALGRNLT+ ETLWFNYS +KSDYFL+CHNILFLF+IFS +PLPL+F+E
Sbjct: 1   MLPFNTLDEAAAALGRNLTYAETLWFNYSANKSDYFLYCHNILFLFVIFSIVPLPLVFVE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
           I+ ++GI KYKIQPKV+L FSE+F CYKDVMRMFFLVVGPLQL+S+PSIQ          
Sbjct: 61  IMRSAGIEKYKIQPKVKLSFSEMFNCYKDVMRMFFLVVGPLQLISYPSIQ---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              MIGIRT LPLPSGWEI  QL+VYFMVEDYTNYWIHRFLH KWGYE IH+VHHEY AP
Sbjct: 121 ---MIGIRTSLPLPSGWEIFLQLLVYFMVEDYTNYWIHRFLHGKWGYEKIHRVHHEYAAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAE+LILGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPW+ TK
Sbjct: 181 IGFAAPYAHWAEILILGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWTPTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKVSILFSPPP 300
           +IPFYGG+D+HDYHHYVGGQS SNFASVFTYCD+IYGTDKGYRYQKK+L K+        
Sbjct: 241 YIPFYGGADYHDYHHYVGGQSQSNFASVFTYCDFIYGTDKGYRYQKKVLAKL-------- 300

Query: 301 PPSPRKLILKEEVKSSEGSYYSTAEDVKVD 331
                K  L+ + + + GSYY   +D+K D
Sbjct: 301 -----KEGLRSDGEQNGGSYY--VQDLKSD 302

BLAST of CmoCh16G010840 vs. TrEMBL
Match: V4TJW5_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10021364mg PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 1.9e-137
Identity = 230/292 (78.77%), Postives = 255/292 (87.33%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLP+ ++  A  ALGRNLTF ETLW+NYS +KSDYFL+CHNILFLFL+FS  PLP + +E
Sbjct: 1   MLPYSTIEDAAAALGRNLTFAETLWYNYSANKSDYFLYCHNILFLFLVFSVAPLPFVVIE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
            L +    KYKIQPKVRL FSE+ RCYKDVMRMFFLVVGPLQLVSFPS+Q          
Sbjct: 61  SLRSDSFDKYKIQPKVRLSFSEMVRCYKDVMRMFFLVVGPLQLVSFPSVQ---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              M+GIRTGLPLPSGWEI++QLVVYFMVEDYTNYWIHRFLHCKWGYE IH+VHHEYTAP
Sbjct: 121 ---MVGIRTGLPLPSGWEILAQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAE+LILGIPSFLGPAM PGHMITFWLWIALRQIEAIDTHSGYDFPW  TK
Sbjct: 181 IGFAAPYAHWAEILILGIPSFLGPAMAPGHMITFWLWIALRQIEAIDTHSGYDFPWGFTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKV 293
           +IPFYGG+D+HDYHHYVG QSHSNFASVFTYCD++YGTDKGYRYQKK+L+K+
Sbjct: 241 YIPFYGGADYHDYHHYVGEQSHSNFASVFTYCDFLYGTDKGYRYQKKLLRKM 279

BLAST of CmoCh16G010840 vs. TrEMBL
Match: A0A067FJY9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g021962mg PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 2.5e-137
Identity = 230/292 (78.77%), Postives = 255/292 (87.33%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLP+ ++  A  ALGRNLTF ETLW+NYS +KSDYFL+CHNILFLFL+FS  PLP + +E
Sbjct: 1   MLPYATIEDAAAALGRNLTFAETLWYNYSANKSDYFLYCHNILFLFLVFSVAPLPFVVIE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
            L +    KYKIQPKVRL FSE+ RCYKDVMRMFFLVVGPLQLVSFPS+Q          
Sbjct: 61  SLRSDSFDKYKIQPKVRLSFSEMVRCYKDVMRMFFLVVGPLQLVSFPSVQ---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              M+GIRTGLPLPSGWEI++QLVVYFMVEDYTNYWIHRFLHCKWGYE IH+VHHEYTAP
Sbjct: 121 ---MVGIRTGLPLPSGWEILAQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAE+LILGIPSFLGPAM PGHMITFWLWIALRQIEAIDTHSGYDFPW  TK
Sbjct: 181 IGFAAPYAHWAEILILGIPSFLGPAMAPGHMITFWLWIALRQIEAIDTHSGYDFPWGFTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKV 293
           +IPFYGG+D+HDYHHYVG QSHSNFASVFTYCD++YGTDKGYRYQKK+L+K+
Sbjct: 241 YIPFYGGADYHDYHHYVGEQSHSNFASVFTYCDFLYGTDKGYRYQKKLLRKM 279

BLAST of CmoCh16G010840 vs. TrEMBL
Match: V7AMK5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_010G081300g PE=3 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 6.1e-136
Identity = 229/296 (77.36%), Postives = 257/296 (86.82%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLP+ S+SA + ALGRNLTF ETLWFNYS  KSDYFL+CHNILFLFLIFS +P+PL+FLE
Sbjct: 1   MLPYASISAVEAALGRNLTFAETLWFNYSATKSDYFLYCHNILFLFLIFSLVPMPLVFLE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
           +   S    YKIQPKVRL   E+FRCYKDVMRMFF +VGPLQLVS+P+IQ          
Sbjct: 61  LKRFSFFDSYKIQPKVRLSLEEMFRCYKDVMRMFFFIVGPLQLVSYPTIQ---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              MIGIRTGLPLPSGWEI++QL+VYF+VEDYTNYWIHRFLH  WGYE IH+VHHEY AP
Sbjct: 121 ---MIGIRTGLPLPSGWEILAQLLVYFLVEDYTNYWIHRFLHNGWGYEKIHRVHHEYPAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAE+LILGIPSFLGPAMVPGH++TFWLWIALRQIEAIDTHSGYDFPWS+TK
Sbjct: 181 IGFAAPYAHWAEILILGIPSFLGPAMVPGHIVTFWLWIALRQIEAIDTHSGYDFPWSITK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKVSILF 297
           +IPFYGG+++HDYHHYVG QSHSNFASVFTYCDYIYGTDKGYRYQKKIL+K  + +
Sbjct: 241 YIPFYGGAEYHDYHHYVGKQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKEDLTY 283

BLAST of CmoCh16G010840 vs. TAIR10
Match: AT4G22756.1 (AT4G22756.1 sterol C4-methyl oxidase 1-2)

HSP 1 Score: 460.3 bits (1183), Expect = 1.0e-129
Identity = 212/294 (72.11%), Postives = 252/294 (85.71%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +AL RNLT+ ETLWF+YS  KSDY+L+CHNILFLFLIFS +PLPL+F+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  ILHASG--IHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFR 120
              ++    ++YKIQPKV+  FS +F+CYKDVM+MF LVVGPLQLVS+PSIQ        
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQ-------- 120

Query: 121 DFYPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYT 180
                MI IR+GLPLPS  EIV+QLVVYF+VEDYTNYW+HRF HCKWGYE  H +HHEYT
Sbjct: 121 -----MIEIRSGLPLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYT 180

Query: 181 APIGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSL 240
           APIG+AAPYAHWAEVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAI+THSGYDFPWSL
Sbjct: 181 APIGYAAPYAHWAEVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSL 240

Query: 241 TKFIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKV 293
           TK+IPFYGG+++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++
Sbjct: 241 TKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQM 281

BLAST of CmoCh16G010840 vs. TAIR10
Match: AT4G12110.1 (AT4G12110.1 sterol-4alpha-methyl oxidase 1-1)

HSP 1 Score: 450.3 bits (1157), Expect = 1.0e-126
Identity = 208/294 (70.75%), Postives = 253/294 (86.05%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +ALGRNLT  ETLWF+YS  KSDY+L+CHNILFLFL+FS +PLPL+F+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  ILH-ASGI-HKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFR 120
           +   ASG+ ++YKIQPKV    S++F+CYKDVM MF LVVGPLQLVS+PSIQ        
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQ-------- 120

Query: 121 DFYPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYT 180
                MI IR+GLPLP+  E++SQLVVYF++EDYTNYW+HRF H KWGY+ IH+VHHEYT
Sbjct: 121 -----MIEIRSGLPLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYT 180

Query: 181 APIGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSL 240
           APIG+AAPYAHWAEVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAI+THSGYDFPWS 
Sbjct: 181 APIGYAAPYAHWAEVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSP 240

Query: 241 TKFIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKV 293
           TK+IPFYGG+++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++
Sbjct: 241 TKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQI 281

BLAST of CmoCh16G010840 vs. TAIR10
Match: AT4G22753.1 (AT4G22753.1 sterol 4-alpha methyl oxidase 1-3)

HSP 1 Score: 419.9 bits (1078), Expect = 1.5e-117
Identity = 190/289 (65.74%), Postives = 239/289 (82.70%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +ALGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
                   ++KIQ KV+   S++F+CYK+VM++F LVVG LQ+VS+PSIQ          
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQ---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              M+GIR+GLPLPS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYE IH++HHEYT+P
Sbjct: 121 ---MVGIRSGLPLPSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IG+A+PYAHWAE+LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK
Sbjct: 181 IGYASPYAHWAEILILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL 290
            IPFYGG ++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L
Sbjct: 241 LIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLL 274

BLAST of CmoCh16G010840 vs. TAIR10
Match: AT1G07420.1 (AT1G07420.1 sterol 4-alpha-methyl-oxidase 2-1)

HSP 1 Score: 216.5 bits (550), Expect = 2.5e-56
Identity = 113/271 (41.70%), Postives = 156/271 (57.56%), Query Frame = 1

Query: 19  TFFETLWFNYSHDKSDYFLFCHNILFLF-LIFSFIPLPLIFLEILHASGIHKYKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP IFLE      + KYKIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDFYPLMIGIRTGLPLPSGW 138
            P ++  +C   ++   F V  PL L S+P  +              +G+R+  PLPS  
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRA-------------MGMRSSFPLPSWK 122

Query: 139 EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEVLILG 198
           E+ +Q++ YF++ED+  YW HR LH KW Y+N+H VHHEY  P G  + YAH AE+L LG
Sbjct: 123 EVSAQILFYFIIEDFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLG 182

Query: 199 IPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDYHHYV 258
             + +GPA+   H+IT WLW+ LR +E ++ H GY FPWSL+ F+P YGG+D HDYHH +
Sbjct: 183 FATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRL 242

Query: 259 GGQSHSNFASVFTYCDYIYGTDKGYRYQKKI 289
                 N++S F Y D+I+GTDKGYR  K +
Sbjct: 243 LYTKSGNYSSTFVYMDWIFGTDKGYRRLKTL 257

BLAST of CmoCh16G010840 vs. TAIR10
Match: AT2G29390.1 (AT2G29390.1 sterol 4-alpha-methyl-oxidase 2-2)

HSP 1 Score: 198.0 bits (502), Expect = 9.3e-51
Identity = 112/270 (41.48%), Postives = 151/270 (55.93%), Query Frame = 1

Query: 33  SDYFLFCHNILFLF-LIFSFIPLPLIFLEILHASGIHKYKIQPKVRLPFSEIFRCYKDVM 92
           SD+ L C     L   +F    LP IFLE      +  YKIQ K   P ++  +C   ++
Sbjct: 17  SDFQLACIGSFILHESVFFLSGLPYIFLE--RTGFLSNYKIQTKSNTPEAQ-GKCIARLL 76

Query: 93  RMFFLVVGPLQLVSFPSIQVNLGFRFRDFYPLMIGIRTGLPLPSGWEIVS-QLVVYFMVE 152
                V  PL + S+P       FRF       +G+ +  PLPS W++VS Q++ YF++E
Sbjct: 77  LYHCCVNLPLMMASYPV------FRF-------MGMESSFPLPS-WKVVSAQILFYFIIE 136

Query: 153 DYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGH 212
           D+  YW HR LH KW Y+N+H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 137 DFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 196

Query: 213 MITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGS------------DHHDYHHYVG 272
           +IT WLW+ LR IE ++ H GY FPWS + F+P YGGS            D HDYHH + 
Sbjct: 197 LITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLL 256

Query: 273 GQSHSNFASVFTYCDYIYGTDKGYRYQKKI 289
                N++S F Y D+I+GTDKGYR  K +
Sbjct: 257 YTKSGNYSSTFVYMDWIFGTDKGYRKLKAL 269

BLAST of CmoCh16G010840 vs. NCBI nr
Match: gi|778675550|ref|XP_011650426.1| (PREDICTED: methylsterol monooxygenase 1-2-like [Cucumis sativus])

HSP 1 Score: 575.5 bits (1482), Expect = 6.0e-161
Identity = 282/330 (85.45%), Postives = 295/330 (89.39%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQ +LGRNLTFFETLWFNYS+DKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
           +LHA+GIHKYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+          
Sbjct: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIK---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              MIGIRTGLPLPSG+EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH VHHEYTAP
Sbjct: 121 ---MIGIRTGLPLPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK
Sbjct: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKVSILFSPPP 300
           FIPFYGG+DHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+K         
Sbjct: 241 FIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQK--------- 300

Query: 301 PPSPRKLILKEEVKSSEGSYYSTAEDVKVD 331
                   LKEEVK+SE SYY+TA++VK D
Sbjct: 301 --------LKEEVKNSEESYYNTAQNVKSD 300

BLAST of CmoCh16G010840 vs. NCBI nr
Match: gi|659096077|ref|XP_008448914.1| (PREDICTED: methylsterol monooxygenase 1-2-like [Cucumis melo])

HSP 1 Score: 572.4 bits (1474), Expect = 5.1e-160
Identity = 283/331 (85.50%), Postives = 294/331 (88.82%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPFHSLSAAQ +LGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
           +LHASGI KYKIQPKVRLPF+EIFRCYKDVMRMFFLVVGPLQLVSFPSI+          
Sbjct: 61  LLHASGIRKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIK---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              MIGIRTGLPLPSG+EIVSQLVVYFMVEDYTNYWIHRFLHCKWGYE IH VHHEYTAP
Sbjct: 121 ---MIGIRTGLPLPSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK
Sbjct: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKVSILFSPPP 300
           FIPFYGG+DHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL+K         
Sbjct: 241 FIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQK--------- 300

Query: 301 PPSPRKLILKEEVKSS-EGSYYSTAEDVKVD 331
                   LKEEVK+S E SYY+TA++VK D
Sbjct: 301 --------LKEEVKNSEESSYYNTAQNVKSD 301

BLAST of CmoCh16G010840 vs. NCBI nr
Match: gi|1009157042|ref|XP_015896561.1| (PREDICTED: methylsterol monooxygenase 1-1 [Ziziphus jujuba])

HSP 1 Score: 507.7 bits (1306), Expect = 1.5e-140
Identity = 246/334 (73.65%), Postives = 278/334 (83.23%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLP+ SL+ A  ALGRNLTF ETLWFNYS  KSDYFL+CHNILFLFLIFS +PLPL+F+E
Sbjct: 1   MLPYQSLAEATSALGRNLTFAETLWFNYSARKSDYFLYCHNILFLFLIFSVVPLPLVFIE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
           ++  +G  +YKIQPKVRL FSE+ RCYKDVMRMFFLVVGPLQLVSFPS++          
Sbjct: 61  LMRIAGFDRYKIQPKVRLSFSEMSRCYKDVMRMFFLVVGPLQLVSFPSVK---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              MIGIRTGLPLPSGWEI+SQL+VYF+VEDYTNYWIHRFLH KWGYE IH+VHHEY AP
Sbjct: 121 ---MIGIRTGLPLPSGWEILSQLLVYFVVEDYTNYWIHRFLHNKWGYEKIHRVHHEYAAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAE+LILGIPSFLGPAMVPGHMITFWLWIALRQ+EAI+THSGYDFPWS+TK
Sbjct: 181 IGFAAPYAHWAEILILGIPSFLGPAMVPGHMITFWLWIALRQVEAIETHSGYDFPWSITK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKVSILFSPPP 300
           +IPFYGG+++HDYHHYVGGQS SNFASVFTYCDYIYGTDKGYRYQKKILKK         
Sbjct: 241 YIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKK--------- 300

Query: 301 PPSPRKLILKEEVKSSE----GSYYSTAEDVKVD 331
                   LKEE++SS+    GSY+ + + +K D
Sbjct: 301 --------LKEELESSDTQNGGSYHISTQSLKSD 304

BLAST of CmoCh16G010840 vs. NCBI nr
Match: gi|802741448|ref|XP_012087086.1| (PREDICTED: methylsterol monooxygenase 1-1-like [Jatropha curcas])

HSP 1 Score: 499.2 bits (1284), Expect = 5.5e-138
Identity = 241/330 (73.03%), Postives = 274/330 (83.03%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLPF++L  A  ALGRNLT+ ETLWFNYS +KSDYFL+CHNILFLF+IFS +PLPL+F+E
Sbjct: 1   MLPFNTLDEAAAALGRNLTYAETLWFNYSANKSDYFLYCHNILFLFVIFSIVPLPLVFVE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
           I+ ++GI KYKIQPKV+L FSE+F CYKDVMRMFFLVVGPLQL+S+PSIQ          
Sbjct: 61  IMRSAGIEKYKIQPKVKLSFSEMFNCYKDVMRMFFLVVGPLQLISYPSIQ---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              MIGIRT LPLPSGWEI  QL+VYFMVEDYTNYWIHRFLH KWGYE IH+VHHEY AP
Sbjct: 121 ---MIGIRTSLPLPSGWEIFLQLLVYFMVEDYTNYWIHRFLHGKWGYEKIHRVHHEYAAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAE+LILGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPW+ TK
Sbjct: 181 IGFAAPYAHWAEILILGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWTPTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKVSILFSPPP 300
           +IPFYGG+D+HDYHHYVGGQS SNFASVFTYCD+IYGTDKGYRYQKK+L K+        
Sbjct: 241 YIPFYGGADYHDYHHYVGGQSQSNFASVFTYCDFIYGTDKGYRYQKKVLAKL-------- 300

Query: 301 PPSPRKLILKEEVKSSEGSYYSTAEDVKVD 331
                K  L+ + + + GSYY   +D+K D
Sbjct: 301 -----KEGLRSDGEQNGGSYY--VQDLKSD 302

BLAST of CmoCh16G010840 vs. NCBI nr
Match: gi|567895868|ref|XP_006440422.1| (hypothetical protein CICLE_v10021364mg [Citrus clementina])

HSP 1 Score: 496.9 bits (1278), Expect = 2.7e-137
Identity = 230/292 (78.77%), Postives = 255/292 (87.33%), Query Frame = 1

Query: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLP+ ++  A  ALGRNLTF ETLW+NYS +KSDYFL+CHNILFLFL+FS  PLP + +E
Sbjct: 1   MLPYSTIEDAAAALGRNLTFAETLWYNYSANKSDYFLYCHNILFLFLVFSVAPLPFVVIE 60

Query: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQVNLGFRFRDF 120
            L +    KYKIQPKVRL FSE+ RCYKDVMRMFFLVVGPLQLVSFPS+Q          
Sbjct: 61  SLRSDSFDKYKIQPKVRLSFSEMVRCYKDVMRMFFLVVGPLQLVSFPSVQ---------- 120

Query: 121 YPLMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAP 180
              M+GIRTGLPLPSGWEI++QLVVYFMVEDYTNYWIHRFLHCKWGYE IH+VHHEYTAP
Sbjct: 121 ---MVGIRTGLPLPSGWEILAQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHRVHHEYTAP 180

Query: 181 IGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTK 240
           IGFAAPYAHWAE+LILGIPSFLGPAM PGHMITFWLWIALRQIEAIDTHSGYDFPW  TK
Sbjct: 181 IGFAAPYAHWAEILILGIPSFLGPAMAPGHMITFWLWIALRQIEAIDTHSGYDFPWGFTK 240

Query: 241 FIPFYGGSDHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKV 293
           +IPFYGG+D+HDYHHYVG QSHSNFASVFTYCD++YGTDKGYRYQKK+L+K+
Sbjct: 241 YIPFYGGADYHDYHHYVGEQSHSNFASVFTYCDFLYGTDKGYRYQKKLLRKM 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SMO12_ARATH1.8e-12872.11Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana GN=SMO1-2 PE=2 SV=1[more]
SMO11_ARATH1.8e-12570.75Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana GN=SMO1-1 PE=1 SV=1[more]
SMO13_ARATH2.7e-11665.74Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana GN=SMO1-3 PE=2 SV=1[more]
SMO22_ARATH4.5e-5541.70Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana GN=SMO2-2 PE=2 SV=1[more]
SMO21_ARATH1.6e-4941.48Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana GN=SMO2-1 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0L5P8_CUCSA4.2e-16185.45Uncharacterized protein OS=Cucumis sativus GN=Csa_3G036480 PE=3 SV=1[more]
A0A067JRW8_JATCU3.8e-13873.03Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20744 PE=3 SV=1[more]
V4TJW5_9ROSI1.9e-13778.77Uncharacterized protein OS=Citrus clementina GN=CICLE_v10021364mg PE=3 SV=1[more]
A0A067FJY9_CITSI2.5e-13778.77Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g021962mg PE=3 SV=1[more]
V7AMK5_PHAVU6.1e-13677.36Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_010G081300g PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G22756.11.0e-12972.11 sterol C4-methyl oxidase 1-2[more]
AT4G12110.11.0e-12670.75 sterol-4alpha-methyl oxidase 1-1[more]
AT4G22753.11.5e-11765.74 sterol 4-alpha methyl oxidase 1-3[more]
AT1G07420.12.5e-5641.70 sterol 4-alpha-methyl-oxidase 2-1[more]
AT2G29390.19.3e-5141.48 sterol 4-alpha-methyl-oxidase 2-2[more]
Match NameE-valueIdentityDescription
gi|778675550|ref|XP_011650426.1|6.0e-16185.45PREDICTED: methylsterol monooxygenase 1-2-like [Cucumis sativus][more]
gi|659096077|ref|XP_008448914.1|5.1e-16085.50PREDICTED: methylsterol monooxygenase 1-2-like [Cucumis melo][more]
gi|1009157042|ref|XP_015896561.1|1.5e-14073.65PREDICTED: methylsterol monooxygenase 1-1 [Ziziphus jujuba][more]
gi|802741448|ref|XP_012087086.1|5.5e-13873.03PREDICTED: methylsterol monooxygenase 1-1-like [Jatropha curcas][more]
gi|567895868|ref|XP_006440422.1|2.7e-13778.77hypothetical protein CICLE_v10021364mg [Citrus clementina][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006694Fatty_acid_hydroxylase
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0006633fatty acid biosynthetic process
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0004497 monooxygenase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G010840.1CmoCh16G010840.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 144..278
score: 1.1
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 1..291
score: 7.3E
NoneNo IPR availablePANTHERPTHR11863:SF44METHYLSTEROL MONOOXYGENASE 1-1-RELATEDcoord: 1..291
score: 7.3E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh16G010840CmoCh06G007730Cucurbita moschata (Rifu)cmocmoB291