CmoCh13G004350 (gene) Cucurbita moschata (Rifu)

NameCmoCh13G004350
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionPhloem protein 2
LocationCmo_Chr13 : 5475885 .. 5476941 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGCATGGCTTATCTTCAAAATATTGTACCAAAGAAGGGGTTATATATACCCCTTCTTCCCTCTCACTTTAACTCATATCTCAATTCAGTTCATAAAAAAGAAGGCACTATAATGGACAACAAAGAGAGGGAAGCCAGAGAGAAATTAGGAGGAGAAGTGAAGCTTGGCCATTGCTTGGATGTTATTTTGAAGAATGCTGACGTAGCACTGCACTATCCTTCCTTCGTTAAGCTTTATGACCAACTTGTTGCTGGGATCCTCCTAAACAAGGGAGCTATAGTAAGTGCAACTATATATATCAATTTCAACTTATTTTACTCAGCTTTATATATCATAAACATATTAAATCAAAATATTTTCTTTGTTTACTTTTCACACAGAGGTACATCTTTGATAAGAAGTTAAACAGCAATTGGTACTTTATATTTGCAAGGGCTCTCTCAATCGCTTGGATTGAAGATAAAAGATACTGGAAATGGGGATCCTGGTATAATTTTTAACTAATTTTTCAAGCGAAAAAAATGATAAAAAGTTAATTTCTTGATCTCTCTCACTCGAGTGTCTAAAAACTTGCAGTGGAGGTAGTGAAGTTGCAGAACTTATTGAAGTATCTTGGCTGGACATTCGTGGAAAGATCAATGAGTCTATGCTCTCACCAAATGCTGTGTATGAAGTAGCACTTCACGTACAGCTAAATGATAGAGCCTCCGGATGGGATGCTCCTCTGAACATTGAATTGAAGAAGCCAGATGGGAGCAAGATAGTGCGCCAGGAATGCCTGTCAGGGAAGCCACGAAACCAGTGGTTTGAGATTGTTGTTGAGTACAAGGTAGGTAACCATGGCTGTGGAAGTAGTGGCGAGATCGAGTTTGCCTTTTATGAACATGGAGGGCATTGGAAGAGAGGGCTGCTCGTGAAAGGTGTTCGGATTGGAGCAAAGGGATGTGGTTGCTCATGATCAAAATAGTCTTGGTGTACACAATCTCTCTCTCTCAAACTCAAAGTACACTTATTTTGATGTTGAGGAACGATAGTTTGTGTTATAATCAAATATGA

mRNA sequence

GTGCATGGCTTATCTTCAAAATATTGTACCAAAGAAGGGGTTATATATACCCCTTCTTCCCTCTCACTTTAACTCATATCTCAATTCAGTTCATAAAAAAGAAGGCACTATAATGGACAACAAAGAGAGGGAAGCCAGAGAGAAATTAGGAGGAGAAGTGAAGCTTGGCCATTGCTTGGATGTTATTTTGAAGAATGCTGACGTAGCACTGCACTATCCTTCCTTCGTTAAGCTTTATGACCAACTTGTTGCTGGGATCCTCCTAAACAAGGGAGCTATAAGGTACATCTTTGATAAGAAGTTAAACAGCAATTGGTACTTTATATTTGCAAGGGCTCTCTCAATCGCTTGGATTGAAGATAAAAGATACTGGAAATGGGGATCCTGTGGAGGTAGTGAAGTTGCAGAACTTATTGAAGTATCTTGGCTGGACATTCGTGGAAAGATCAATGAGTCTATGCTCTCACCAAATGCTGTGTATGAAGTAGCACTTCACGTACAGCTAAATGATAGAGCCTCCGGATGGGATGCTCCTCTGAACATTGAATTGAAGAAGCCAGATGGGAGCAAGATAGTGCGCCAGGAATGCCTGTCAGGGAAGCCACGAAACCAGTGGTTTGAGATTGTTGTTGAGTACAAGGTAGAGAGGGCTGCTCGTGAAAGGTGTTCGGATTGGAGCAAAGGGATGTGGTTGCTCATGATCAAAATAGTCTTGGTGTACACAATCTCTCTCTCTCAAACTCAAAGTACACTTATTTTGATGTTGAGGAACGATAGTTTGTGTTATAATCAAATATGA

Coding sequence (CDS)

ATGGCTTATCTTCAAAATATTGTACCAAAGAAGGGGTTATATATACCCCTTCTTCCCTCTCACTTTAACTCATATCTCAATTCAGTTCATAAAAAAGAAGGCACTATAATGGACAACAAAGAGAGGGAAGCCAGAGAGAAATTAGGAGGAGAAGTGAAGCTTGGCCATTGCTTGGATGTTATTTTGAAGAATGCTGACGTAGCACTGCACTATCCTTCCTTCGTTAAGCTTTATGACCAACTTGTTGCTGGGATCCTCCTAAACAAGGGAGCTATAAGGTACATCTTTGATAAGAAGTTAAACAGCAATTGGTACTTTATATTTGCAAGGGCTCTCTCAATCGCTTGGATTGAAGATAAAAGATACTGGAAATGGGGATCCTGTGGAGGTAGTGAAGTTGCAGAACTTATTGAAGTATCTTGGCTGGACATTCGTGGAAAGATCAATGAGTCTATGCTCTCACCAAATGCTGTGTATGAAGTAGCACTTCACGTACAGCTAAATGATAGAGCCTCCGGATGGGATGCTCCTCTGAACATTGAATTGAAGAAGCCAGATGGGAGCAAGATAGTGCGCCAGGAATGCCTGTCAGGGAAGCCACGAAACCAGTGGTTTGAGATTGTTGTTGAGTACAAGGTAGAGAGGGCTGCTCGTGAAAGGTGTTCGGATTGGAGCAAAGGGATGTGGTTGCTCATGATCAAAATAGTCTTGGTGTACACAATCTCTCTCTCTCAAACTCAAAGTACACTTATTTTGATGTTGAGGAACGATAGTTTGTGTTATAATCAAATATGA
BLAST of CmoCh13G004350 vs. Swiss-Prot
Match: LEC_LUFAC (Lectin OS=Luffa acutangula PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.5e-40
Identity = 87/169 (51.48%), Postives = 109/169 (64.50%), Query Frame = 1

Query: 46  EKLGGEVKLGHCLDVILKNADVALH-YPSFVKLYDQLVAGILLNKGAIRYIFDKKLNSNW 105
           E +GGEVK+GH L+ ILK  DV ++  PSF+KLYDQ+ AGI LN    RY FDK   SN 
Sbjct: 2   ELVGGEVKVGHNLEAILKGLDVDVYSVPSFIKLYDQVTAGIFLNNRTKRYWFDKNAESNC 61

Query: 106 YFIFARALSIAWIEDKRYWKWGSC---GGS-EVAELIEVSWLDIRGKINESMLSPNAVYE 165
           + ++AR L I W +DKRYW+W      G + EVAELI+V WL+I G I  S+LSP   YE
Sbjct: 62  FMLYARDLLITWSQDKRYWRWNPFQEHGNTLEVAELIDVCWLNIVGNIETSVLSPGISYE 121

Query: 166 VALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVV 210
            A  V L + ASGW  P++++LK PDGS+   Q  L  KPR  WF I V
Sbjct: 122 AAFEVMLTNSASGWRIPVDVKLKMPDGSEQESQVNLQDKPRGVWFFISV 170

BLAST of CmoCh13G004350 vs. Swiss-Prot
Match: P2A01_ARATH (Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana GN=PP2A1 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.2e-23
Identity = 72/202 (35.64%), Postives = 108/202 (53.47%), Query Frame = 1

Query: 52  VKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLN-KGAIRYIFDKKLNSNWYFIFAR 111
           VK  H  + IL++AD  +   S V L +QL +G+ L  K  I+Y  D++ NSN + +FA+
Sbjct: 43  VKSPHNCEAILRDADPPISLSS-VNLSEQLRSGVFLKPKKQIKYWVDER-NSNCFMLFAK 102

Query: 112 ALSIAWIEDKRYWKWGSCGGS-----EVAELIEVSWLDIRGKINESMLSPNAVYEVALHV 171
            LSI W +D  YW W +   S     E   L  V WLDI GK +   L+P  VYEV   V
Sbjct: 103 NLSITWSDDVNYWTWFTEKESPNENVEAVGLKNVCWLDITGKFDTRNLTPGIVYEVVFKV 162

Query: 172 QLNDRASGWDAPLNIELKKPDGSKIVRQECLSGK--PRNQWFEI-VVEYKVERAA----- 231
           +L D A GWD P+N++L  P+G +  +++ +S +  PR +W ++ V E+  E++A     
Sbjct: 163 KLEDPAYGWDTPVNLKLVLPNGKEKPQEKKVSLRELPRYKWVDVRVGEFVPEKSAAGEIT 222

Query: 232 ----RERCSDWSKGMWLLMIKI 236
                     W KG+ L  + I
Sbjct: 223 FSMYEHAAGVWKKGLSLKGVAI 242

BLAST of CmoCh13G004350 vs. Swiss-Prot
Match: P2A04_ARATH (Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana GN=PP2A4 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.8e-14
Identity = 43/112 (38.39%), Postives = 57/112 (50.89%), Query Frame = 1

Query: 105 YFIFARALSIAWIEDKRYWKW-------GSCGGSEVAELIEVSWLDIRGKINESMLSPNA 164
           + I+AR LSIAW +   YW W        S    + A L  V WLD+ GK +   L+   
Sbjct: 12  HMIYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLET 71

Query: 165 VYEVALHVQLNDRASGWDAPLNIELKKPDGSK--IVRQECLSGKPRNQWFEI 208
            YEV   V+L D ASGW+ P+N++L  PDG K    R  CL      +W +I
Sbjct: 72  TYEVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDI 123

BLAST of CmoCh13G004350 vs. Swiss-Prot
Match: P2B15_ARATH (F-box protein PP2-B15 OS=Arabidopsis thaliana GN=PP2B15 PE=2 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 1.3e-12
Identity = 46/142 (32.39%), Postives = 67/142 (47.18%), Query Frame = 1

Query: 60  VILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIFDKKLNSNWYFIFARALSIAWIED 119
           VI ++ D    + S  +LY  L   IL++ G   +  +K      Y + +R LSI W + 
Sbjct: 51  VISRSTDPHRIFSSKKELYRCLCESILIDNGRKIFKIEKLSGKISYILSSRDLSITWSDQ 110

Query: 120 KRYWKWGSCGGSEVAE---LIEVSWLDIRGKINESMLSPNAVYEVALHVQLNDRASGWD- 179
           + YW W     S  +E   LI   WL+I GKI    LSPN  Y   L +++  RA G D 
Sbjct: 111 RHYWSWSPRSDSRFSEGVQLIMTDWLEIIGKIQTGALSPNTNYGAYLIMKVTSRAYGLDL 170

Query: 180 APLNIELKKPDGSKIVRQECLS 198
            P    +K  +G K ++   LS
Sbjct: 171 VPAETSIKVGNGEKKIKSTYLS 192

BLAST of CmoCh13G004350 vs. Swiss-Prot
Match: P2A07_ARATH (Uncharacterized protein PHLOEM PROTEIN 2-LIKE A7 OS=Arabidopsis thaliana GN=PP2A7 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.9e-11
Identity = 42/124 (33.87%), Postives = 63/124 (50.81%), Query Frame = 1

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSC------GGSEVAELIEVSWLDIRGKINE 156
           +K+ NS  + + AR L ++  E    W W S          EVA L EV WL + G  + 
Sbjct: 169 EKEANSKCFMVPARKLQMSHSEKLINWTWSSIYETPNDAAIEVAMLNEVHWLHMSGNFHT 228

Query: 157 SMLSPNAVYEVALHVQLNDRASGWDAPLNIELK--KPDGSKIVRQ-----ECLSGKPRNQ 208
             L+P   YEV   V L+D +SGW+ P+N+ LK   PDG++ +++     EC  G+    
Sbjct: 229 RNLTPGTKYEVVFLVSLDDTSSGWEQPVNLNLKVINPDGTESLQERETSLECHIGE---N 288

BLAST of CmoCh13G004350 vs. TrEMBL
Match: Q9LLT3_CUCMO (Phloem protein 2 OS=Cucurbita moschata GN=pp2 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.1e-92
Identity = 173/210 (82.38%), Postives = 181/210 (86.19%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF
Sbjct: 1   MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKK NSNWYFIFARALSIAWIEDKRYWKWGSC   ++AELIEVSWLDIRGKINESMLSPN
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC--IKIAELIEVSWLDIRGKINESMLSPN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKV--- 216
            VYEVAL VQLNDRASGWDAPLNIELKKPDGSKIVRQECL GKPRNQWFEIVVE+KV   
Sbjct: 121 IVYEVALQVQLNDRASGWDAPLNIELKKPDGSKIVRQECLLGKPRNQWFEIVVEFKVGNH 180

Query: 217 --------ERAARERCSDWSKGMWLLMIKI 236
                   E A  E    W +G+ +  ++I
Sbjct: 181 GCGSSGEIEFAFYEHGGHWKRGLLVKGVRI 208

BLAST of CmoCh13G004350 vs. TrEMBL
Match: Q7DLM2_CUCMA (PP2 lectin polypeptide OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.0e-92
Identity = 169/210 (80.48%), Postives = 183/210 (87.14%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           MDNKE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKKLNS+WYFIFARALSIAWIEDKRYWKWGSCG SEVAELIEVSWL+IRGKINESMLSPN
Sbjct: 61  DKKLNSHWYFIFARALSIAWIEDKRYWKWGSCGNSEVAELIEVSWLNIRGKINESMLSPN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKV--- 216
            VYEVAL VQLN RASGW+APLNIELKKPDGSKIVRQECL GKP+NQWFEIVVE+KV   
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIVRQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 217 --------ERAARERCSDWSKGMWLLMIKI 236
                   E +  E    W +G+ +  ++I
Sbjct: 181 GCGSSGEIEFSFFEHGGHWKRGLLVKGVRI 210

BLAST of CmoCh13G004350 vs. TrEMBL
Match: Q42383_CUCMA (Dimeric phloem specific lectin PP2 OS=Cucurbita maxima GN=PP2 PE=2 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 3.7e-91
Identity = 168/210 (80.00%), Postives = 179/210 (85.24%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           MDNKE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKK NSNWYFIFARALSIAWIEDKRYWKWGSCG S VAELIEVSWLDIRGKINESMLS N
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKV--- 216
            VYEVAL VQLN RASGW+APLNIELKKPDGSKI RQECL GKP+NQWFEIVVE+KV   
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 217 --------ERAARERCSDWSKGMWLLMIKI 236
                   E A  E    W +G+ +  ++I
Sbjct: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRI 210

BLAST of CmoCh13G004350 vs. TrEMBL
Match: Q8LK66_9ROSI (Phloem protein 2 OS=Cucurbita digitata GN=PP2 PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 8.2e-91
Identity = 167/210 (79.52%), Postives = 180/210 (85.71%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           MDNKE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKK NSNWYFIFARALSIAWIEDKRYWKWGSCG S VAELIEVSWLDIRGKINESMLS N
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKV--- 216
            VYEVAL VQLN RASGW+APLNIELKKPDGS+I RQECL GKP+NQWFEIVVE+KV   
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSRIARQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 217 --------ERAARERCSDWSKGMWLLMIKI 236
                   E A  E    W++G+ +  ++I
Sbjct: 181 GCGSSGEIEFAFFEHGGAWARGLLVKGVRI 210

BLAST of CmoCh13G004350 vs. TrEMBL
Match: Q8LK67_9ROSI (Phloem lectin OS=Cucurbita argyrosperma subsp. sororia GN=PP2 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.3e-85
Identity = 152/178 (85.39%), Postives = 168/178 (94.38%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           M++KE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MNHKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKK NSNWYFIFARALSIAWIEDKRYWKWGSC  +++AELI+VSWLDIRGKINESMLSPN
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC--NKIAELIQVSWLDIRGKINESMLSPN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKVE 215
            VYEVAL VQLN  ASGW+ P+NIELKKP+GSKI RQECL GKP+NQWFEIV+E+KV+
Sbjct: 121 IVYEVALQVQLNSGASGWNHPMNIELKKPNGSKIERQECLLGKPKNQWFEIVIEFKVD 176

BLAST of CmoCh13G004350 vs. TAIR10
Match: AT4G19840.1 (AT4G19840.1 phloem protein 2-A1)

HSP 1 Score: 111.7 bits (278), Expect = 7.0e-25
Identity = 72/202 (35.64%), Postives = 108/202 (53.47%), Query Frame = 1

Query: 52  VKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLN-KGAIRYIFDKKLNSNWYFIFAR 111
           VK  H  + IL++AD  +   S V L +QL +G+ L  K  I+Y  D++ NSN + +FA+
Sbjct: 43  VKSPHNCEAILRDADPPISLSS-VNLSEQLRSGVFLKPKKQIKYWVDER-NSNCFMLFAK 102

Query: 112 ALSIAWIEDKRYWKWGSCGGS-----EVAELIEVSWLDIRGKINESMLSPNAVYEVALHV 171
            LSI W +D  YW W +   S     E   L  V WLDI GK +   L+P  VYEV   V
Sbjct: 103 NLSITWSDDVNYWTWFTEKESPNENVEAVGLKNVCWLDITGKFDTRNLTPGIVYEVVFKV 162

Query: 172 QLNDRASGWDAPLNIELKKPDGSKIVRQECLSGK--PRNQWFEI-VVEYKVERAA----- 231
           +L D A GWD P+N++L  P+G +  +++ +S +  PR +W ++ V E+  E++A     
Sbjct: 163 KLEDPAYGWDTPVNLKLVLPNGKEKPQEKKVSLRELPRYKWVDVRVGEFVPEKSAAGEIT 222

Query: 232 ----RERCSDWSKGMWLLMIKI 236
                     W KG+ L  + I
Sbjct: 223 FSMYEHAAGVWKKGLSLKGVAI 242

BLAST of CmoCh13G004350 vs. TAIR10
Match: AT4G19850.2 (AT4G19850.2 lectin-related)

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-17
Identity = 45/118 (38.14%), Postives = 66/118 (55.93%), Query Frame = 1

Query: 99  KLNSNWYFIFARALSIAWIEDK--RYWKW-------GSCGGSEVAELIEVSWLDIRGKIN 158
           K   N + ++AR LSI W E +  +YW W        S   +EVA++  V+WL++ GK  
Sbjct: 59  KAKKNCFMLYARDLSITWAESQTNKYWSWFSDLDQTSSDVRTEVAKMERVAWLEVVGKFE 118

Query: 159 ESMLSPNAVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEI 208
              L+PN++YEV   V+L D A GWD  +N +L  P G    R+E ++   RN+W EI
Sbjct: 119 TEKLTPNSLYEVVFVVKLIDSAKGWDFRVNFKLVLPTGETKERRENVNLLERNKWVEI 176

BLAST of CmoCh13G004350 vs. TAIR10
Match: AT1G33920.1 (AT1G33920.1 phloem protein 2-A4)

HSP 1 Score: 79.3 bits (194), Expect = 3.8e-15
Identity = 43/112 (38.39%), Postives = 57/112 (50.89%), Query Frame = 1

Query: 105 YFIFARALSIAWIEDKRYWKW-------GSCGGSEVAELIEVSWLDIRGKINESMLSPNA 164
           + I+AR LSIAW +   YW W        S    + A L  V WLD+ GK +   L+   
Sbjct: 12  HMIYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLET 71

Query: 165 VYEVALHVQLNDRASGWDAPLNIELKKPDGSK--IVRQECLSGKPRNQWFEI 208
            YEV   V+L D ASGW+ P+N++L  PDG K    R  CL      +W +I
Sbjct: 72  TYEVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDI 123

BLAST of CmoCh13G004350 vs. TAIR10
Match: AT1G09155.1 (AT1G09155.1 phloem protein 2-B15)

HSP 1 Score: 75.1 bits (183), Expect = 7.2e-14
Identity = 46/142 (32.39%), Postives = 67/142 (47.18%), Query Frame = 1

Query: 60  VILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIFDKKLNSNWYFIFARALSIAWIED 119
           VI ++ D    + S  +LY  L   IL++ G   +  +K      Y + +R LSI W + 
Sbjct: 51  VISRSTDPHRIFSSKKELYRCLCESILIDNGRKIFKIEKLSGKISYILSSRDLSITWSDQ 110

Query: 120 KRYWKWGSCGGSEVAE---LIEVSWLDIRGKINESMLSPNAVYEVALHVQLNDRASGWD- 179
           + YW W     S  +E   LI   WL+I GKI    LSPN  Y   L +++  RA G D 
Sbjct: 111 RHYWSWSPRSDSRFSEGVQLIMTDWLEIIGKIQTGALSPNTNYGAYLIMKVTSRAYGLDL 170

Query: 180 APLNIELKKPDGSKIVRQECLS 198
            P    +K  +G K ++   LS
Sbjct: 171 VPAETSIKVGNGEKKIKSTYLS 192

BLAST of CmoCh13G004350 vs. TAIR10
Match: AT5G45090.1 (AT5G45090.1 phloem protein 2-A7)

HSP 1 Score: 71.2 bits (173), Expect = 1.0e-12
Identity = 42/124 (33.87%), Postives = 63/124 (50.81%), Query Frame = 1

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSC------GGSEVAELIEVSWLDIRGKINE 156
           +K+ NS  + + AR L ++  E    W W S          EVA L EV WL + G  + 
Sbjct: 169 EKEANSKCFMVPARKLQMSHSEKLINWTWSSIYETPNDAAIEVAMLNEVHWLHMSGNFHT 228

Query: 157 SMLSPNAVYEVALHVQLNDRASGWDAPLNIELK--KPDGSKIVRQ-----ECLSGKPRNQ 208
             L+P   YEV   V L+D +SGW+ P+N+ LK   PDG++ +++     EC  G+    
Sbjct: 229 RNLTPGTKYEVVFLVSLDDTSSGWEQPVNLNLKVINPDGTESLQERETSLECHIGE---N 288

BLAST of CmoCh13G004350 vs. NCBI nr
Match: gi|8307830|gb|AAF74345.1|AF150627_1 (phloem protein 2 [Cucurbita moschata])

HSP 1 Score: 347.8 bits (891), Expect = 1.6e-92
Identity = 173/210 (82.38%), Postives = 181/210 (86.19%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF
Sbjct: 1   MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKK NSNWYFIFARALSIAWIEDKRYWKWGSC   ++AELIEVSWLDIRGKINESMLSPN
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC--IKIAELIEVSWLDIRGKINESMLSPN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKV--- 216
            VYEVAL VQLNDRASGWDAPLNIELKKPDGSKIVRQECL GKPRNQWFEIVVE+KV   
Sbjct: 121 IVYEVALQVQLNDRASGWDAPLNIELKKPDGSKIVRQECLLGKPRNQWFEIVVEFKVGNH 180

Query: 217 --------ERAARERCSDWSKGMWLLMIKI 236
                   E A  E    W +G+ +  ++I
Sbjct: 181 GCGSSGEIEFAFYEHGGHWKRGLLVKGVRI 208

BLAST of CmoCh13G004350 vs. NCBI nr
Match: gi|410437|emb|CAA80364.1| (PP2 lectin polypeptide [Cucurbita maxima])

HSP 1 Score: 347.1 bits (889), Expect = 2.8e-92
Identity = 169/210 (80.48%), Postives = 183/210 (87.14%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           MDNKE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKKLNS+WYFIFARALSIAWIEDKRYWKWGSCG SEVAELIEVSWL+IRGKINESMLSPN
Sbjct: 61  DKKLNSHWYFIFARALSIAWIEDKRYWKWGSCGNSEVAELIEVSWLNIRGKINESMLSPN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKV--- 216
            VYEVAL VQLN RASGW+APLNIELKKPDGSKIVRQECL GKP+NQWFEIVVE+KV   
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIVRQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 217 --------ERAARERCSDWSKGMWLLMIKI 236
                   E +  E    W +G+ +  ++I
Sbjct: 181 GCGSSGEIEFSFFEHGGHWKRGLLVKGVRI 210

BLAST of CmoCh13G004350 vs. NCBI nr
Match: gi|18069|emb|CAA78979.1| (dimeric phloem specific lectin PP2 [Cucurbita maxima])

HSP 1 Score: 342.8 bits (878), Expect = 5.3e-91
Identity = 168/210 (80.00%), Postives = 179/210 (85.24%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           MDNKE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKK NSNWYFIFARALSIAWIEDKRYWKWGSCG S VAELIEVSWLDIRGKINESMLS N
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKV--- 216
            VYEVAL VQLN RASGW+APLNIELKKPDGSKI RQECL GKP+NQWFEIVVE+KV   
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 217 --------ERAARERCSDWSKGMWLLMIKI 236
                   E A  E    W +G+ +  ++I
Sbjct: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRI 210

BLAST of CmoCh13G004350 vs. NCBI nr
Match: gi|21952274|gb|AAM82559.1|AF520583_1 (phloem protein 2 [Cucurbita digitata])

HSP 1 Score: 341.7 bits (875), Expect = 1.2e-90
Identity = 167/210 (79.52%), Postives = 180/210 (85.71%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           MDNKE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKK NSNWYFIFARALSIAWIEDKRYWKWGSCG S VAELIEVSWLDIRGKINESMLS N
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKV--- 216
            VYEVAL VQLN RASGW+APLNIELKKPDGS+I RQECL GKP+NQWFEIVVE+KV   
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSRIARQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 217 --------ERAARERCSDWSKGMWLLMIKI 236
                   E A  E    W++G+ +  ++I
Sbjct: 181 GCGSSGEIEFAFFEHGGAWARGLLVKGVRI 210

BLAST of CmoCh13G004350 vs. NCBI nr
Match: gi|21952272|gb|AAM82558.1|AF520582_1 (phloem lectin [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 323.6 bits (828), Expect = 3.3e-85
Identity = 152/178 (85.39%), Postives = 168/178 (94.38%), Query Frame = 1

Query: 37  MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 96
           M++KE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MNHKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 97  DKKLNSNWYFIFARALSIAWIEDKRYWKWGSCGGSEVAELIEVSWLDIRGKINESMLSPN 156
           DKK NSNWYFIFARALSIAWIEDKRYWKWGSC  +++AELI+VSWLDIRGKINESMLSPN
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC--NKIAELIQVSWLDIRGKINESMLSPN 120

Query: 157 AVYEVALHVQLNDRASGWDAPLNIELKKPDGSKIVRQECLSGKPRNQWFEIVVEYKVE 215
            VYEVAL VQLN  ASGW+ P+NIELKKP+GSKI RQECL GKP+NQWFEIV+E+KV+
Sbjct: 121 IVYEVALQVQLNSGASGWNHPMNIELKKPNGSKIERQECLLGKPKNQWFEIVIEFKVD 176

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
LEC_LUFAC1.5e-4051.48Lectin OS=Luffa acutangula PE=1 SV=1[more]
P2A01_ARATH1.2e-2335.64Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana GN=PP2A1 PE=2 SV=1[more]
P2A04_ARATH6.8e-1438.39Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana GN=PP2A... [more]
P2B15_ARATH1.3e-1232.39F-box protein PP2-B15 OS=Arabidopsis thaliana GN=PP2B15 PE=2 SV=2[more]
P2A07_ARATH1.9e-1133.87Uncharacterized protein PHLOEM PROTEIN 2-LIKE A7 OS=Arabidopsis thaliana GN=PP2A... [more]
Match NameE-valueIdentityDescription
Q9LLT3_CUCMO1.1e-9282.38Phloem protein 2 OS=Cucurbita moschata GN=pp2 PE=2 SV=1[more]
Q7DLM2_CUCMA2.0e-9280.48PP2 lectin polypeptide OS=Cucurbita maxima PE=2 SV=1[more]
Q42383_CUCMA3.7e-9180.00Dimeric phloem specific lectin PP2 OS=Cucurbita maxima GN=PP2 PE=2 SV=1[more]
Q8LK66_9ROSI8.2e-9179.52Phloem protein 2 OS=Cucurbita digitata GN=PP2 PE=2 SV=1[more]
Q8LK67_9ROSI2.3e-8585.39Phloem lectin OS=Cucurbita argyrosperma subsp. sororia GN=PP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G19840.17.0e-2535.64 phloem protein 2-A1[more]
AT4G19850.21.1e-1738.14 lectin-related[more]
AT1G33920.13.8e-1538.39 phloem protein 2-A4[more]
AT1G09155.17.2e-1432.39 phloem protein 2-B15[more]
AT5G45090.11.0e-1233.87 phloem protein 2-A7[more]
Match NameE-valueIdentityDescription
gi|8307830|gb|AAF74345.1|AF150627_11.6e-9282.38phloem protein 2 [Cucurbita moschata][more]
gi|410437|emb|CAA80364.1|2.8e-9280.48PP2 lectin polypeptide [Cucurbita maxima][more]
gi|18069|emb|CAA78979.1|5.3e-9180.00dimeric phloem specific lectin PP2 [Cucurbita maxima][more]
gi|21952274|gb|AAM82559.1|AF520583_11.2e-9079.52phloem protein 2 [Cucurbita digitata][more]
gi|21952272|gb|AAM82558.1|AF520582_13.3e-8585.39phloem lectin [Cucurbita argyrosperma subsp. sororia][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR025886PP2-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0030246 carbohydrate binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G004350.1CmoCh13G004350.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025886Phloem protein 2-likePFAMPF14299PP2coord: 105..235
score: 1.4
NoneNo IPR availablePANTHERPTHR32278FAMILY NOT NAMEDcoord: 1..209
score: 3.8
NoneNo IPR availablePANTHERPTHR32278:SF3SUBFAMILY NOT NAMEDcoord: 1..209
score: 3.8

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None