CmoCh13G003230 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh13G003230
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Pectin lyase-like superfamily protein
Location	Cmo_Chr13 : 3939580 .. 3940971 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGCATGGAAGGATGCATGCACGTCCAATCGCTCTAGTGCGATTGTTATTCCGGGAGGGATATTTAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATTGATAGATTGACATTATGAGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTGGAAAAAGAATGATTGCCATAAACGCATAAATTGTGCCCAACTCCCCATTGTAAGCAATATGCCTTCAGCTTTCCTTCTCAATCTCGTGCATCTTGTTGTTTTTAGACATCTTCTCTACTCTTCTGACATTTATAATTTGGTAAAAGCTCTTCATTTTCTAGACGATTATCTTTAACCATTCTAAAATAAAAAATAAAAAAGCATGTAAAGGAGTGAAATTCTTTATGAGTTTTCGATTTTATTGGGTTCTGTTAAATTTTTTGGCTTCATTACTGATGCTCACAAAATATTATTTGATTATACAAAATGTTTAGAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCTCTAATATCAATATAACTGCACCAGAAAAGAGTCCCAACACTGATGGAATACACATCGGTCGATCAAATAGGATCGCAATCACAATGTCAAAAATTGCAACAGGAGATGATTGCATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTTATCGTAAAGAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATAGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGGTATTGATCGCATTCTCAAAGCTTTGATTGATTTTATCACTATAACTTTCACAAATAAACCACTTACAGAAATTTGTTGTCTCATTTTGATTTTTCAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTGTGCAACTCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAAAGGTCCTATTACATCTCAATGCACGAATGTGAGACCCATCATTTCAGGAAAACAAAACCCTAAAATC

mRNA sequence

AATGCATGGAAGGATGCATGCACGTCCAATCGCTCTAGTGCGATTGTTATTCCGGGAGGGATATTTAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGAGTTTTTGATGGCCAAGGAAAGGCAGCTTGGAAAAAGAATGATTGCCATAAACGCATAAATTGTGCCCAACTCCCCATTAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCTCTAATATCAATATAACTGCACCAGAAAAGAGTCCCAACACTGATGGAATACACATCGGTCGATCAAATAGGATCGCAATCACAATGTCAAAAATTGCAACAGGAGATGATTGCATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTTATCGTAAAGAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATAGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTGTGCAACTCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAAAGGTCCTATTACATCTCAATGCACGAATGTGAGACCCATCATTTCAGGAAAACAAAACCCTAAAATC

Coding sequence (CDS)

AATGCATGGAAGGATGCATGCACGTCCAATCGCTCTAGTGCGATTGTTATTCCGGGAGGGATATTTAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGAGTTTTTGATGGCCAAGGAAAGGCAGCTTGGAAAAAGAATGATTGCCATAAACGCATAAATTGTGCCCAACTCCCCATTAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCTCTAATATCAATATAACTGCACCAGAAAAGAGTCCCAACACTGATGGAATACACATCGGTCGATCAAATAGGATCGCAATCACAATGTCAAAAATTGCAACAGGAGATGATTGCATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTTATCGTAAAGAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATAGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTGTGCAACTCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAAAGGTCCTATTACATCTCAATGCACGAATGTGAGACCCATCATTTCAGGAAAACAAAACCCTAAAATC

Homology

[Close]

BLAST of CmoCh13G003230 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 5.0e-101
Identity = 186/346 (53.76%), Postives = 226/346 (65.32%), Query Frame = 1


Query: 2   AWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR----------- 61
           AWK+AC S   S I++P G F V     +GPC S I  QL GTL+AP             
Sbjct: 9   AWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIKGLGWIN 68

Query: 62  -------------VFDGQGKAAWKKNDCHKRIN-CAQLPIR-------------ITSLDS 121
                        VFDGQGK+AW +NDCHK    C  L +              +T+LDS
Sbjct: 69  LNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILRDVTTLDS 128

Query: 122 KNFHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGS 181
           KNFH+NV+GC NLTF    I+A E S NTDGIHIGRS+ + I  ++I TGDDCISLGDGS
Sbjct: 129 KNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDDCISLGDGS 188

Query: 182 KQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGT 241
           K INITN+TCGPGHGIS+GSLGRY NEE V G+ VKNCTI  + NGVRIKTWP S   G 
Sbjct: 189 KNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPKS-EPGE 248

Query: 242 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKL 301
           A+ M F DI M +V  PILIDQ YCP+NQC  ++PS +K+SK+SFKNI+G+  T  A+KL
Sbjct: 249 ASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTSTTKEAVKL 308

Query: 302 ICSRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 310
           +CS+  PC+ V++ DIDLTY+G  GP TS C N++P I GKQ P I
Sbjct: 309 VCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAI 353

[Close]

BLAST of CmoCh13G003230 vs. Swiss-Prot
Match: PGLR_BRANA (Polygalacturonase OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 5.8e-97
Identity = 173/343 (50.44%), Postives = 221/343 (64.43%), Query Frame = 1


Query: 2   AWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQL-------GGTLQAPKR---- 61
           A+  AC +   S ++IP G FK+ E +  GPC SPI+F L       GG+ Q   R    
Sbjct: 42  AFTSACQAPAPSQVLIPKGDFKLGETVMTGPCKSPIEFTLQGNVKTDGGSTQGKDRWVVF 101

Query: 62  ------------VFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLDSKN 121
                        FDG+G AAWK N+CHK   C +LPI +             TSLD+KN
Sbjct: 102 EKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPISVRFDFVDNAEIKDVTSLDAKN 161

Query: 122 FHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQ 181
           FH NV+   N+TF NI I AP +SPNTDGIH+GR   + I  +KIATGDDCIS+GDG K 
Sbjct: 162 FHFNVISGKNMTFDNIKIIAPAESPNTDGIHLGRCEGVKILNTKIATGDDCISVGDGMKN 221

Query: 182 INITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTAT 241
           + I  V CGPGHGIS+GSLGRY  E+ V  + VKNCT+  T+NG+RIKTWP++A + TA 
Sbjct: 222 LLIEKVVCGPGHGISVGSLGRYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAACTTTAA 281

Query: 242 NMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLIC 301
            + F DII+  VSNPILIDQEYCPWNQCN+  PS IK+  ++F+NIRG+     A+KL+C
Sbjct: 282 GIHFEDIILNKVSNPILIDQEYCPWNQCNKNKPSTIKLVDITFRNIRGTSENKDAVKLLC 341

Query: 302 SRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPK 309
           S+  PC+ V++GDI++ Y G  GP T +CTNV P + G QNPK
Sbjct: 342 SKGHPCENVEIGDINIEYTGPDGPPTFECTNVTPKLVGAQNPK 384

[Close]

BLAST of CmoCh13G003230 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 5.4e-95
Identity = 171/344 (49.71%), Postives = 220/344 (63.95%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           +AWK+AC S   S +VIP G + + +   +GPC +PI+  + GT+QAP            
Sbjct: 51  DAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWV 110

Query: 61  --------------VFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLDS 120
                         +FDGQG  A++KN C  R   ++LP+ I             TS DS
Sbjct: 111 RFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDS 170

Query: 121 KNFHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGS 180
           K FHINV  C N+T   + I AP++SPNTDGIH+G+S  + I  S I TGDDCIS+GDG+
Sbjct: 171 KLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGT 230

Query: 181 KQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGT 240
           K + I  +TCGPGHGISIGSLG++ NEEPVEG+ + NCTI NT+NG RIKTWP     G 
Sbjct: 231 KNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGE-HGGA 290

Query: 241 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKL 300
            + + F DI M NVS+PILIDQ+YCPWN+C +   SK+K+S +SFKNIRG+ A P AIK 
Sbjct: 291 VSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKF 350

Query: 301 ICSRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNP 308
           ICS   PC  V++ DID+ +NG + P TSQC NV+PI SGK NP
Sbjct: 351 ICSGSSPCQNVELADIDIKHNGAE-PATSQCLNVKPITSGKLNP 392

[Close]

BLAST of CmoCh13G003230 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.7e-94
Identity = 170/344 (49.42%), Postives = 219/344 (63.66%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           +AWK+AC S   S +VIP G + + +   +GPC +PI+  + GT+QAP            
Sbjct: 51  DAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWV 110

Query: 61  --------------VFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLDS 120
                         +FDGQG  A++KN C  R   ++LP+ I             TS DS
Sbjct: 111 RFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDS 170

Query: 121 KNFHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGS 180
           K FHINV  C N+T   + I AP++SPNTDGIH+G+S  + I  S I TGDDCIS+GDG+
Sbjct: 171 KLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGT 230

Query: 181 KQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGT 240
           K + I  +TCGPGHGISIGSLG++ NEEPVEG+ + NCTI NT+NG RIKTWP     G 
Sbjct: 231 KNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGE-HGGA 290

Query: 241 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKL 300
            + + F DI M NVS+PILIDQ+YCPWN+C +   SK+K+S +SFKNIRG+ A P AIK 
Sbjct: 291 VSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKF 350

Query: 301 ICSRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNP 308
           ICS   PC  V++ DID+ +NG + P TSQC NV+PI  GK NP
Sbjct: 351 ICSGSSPCQNVELADIDIQHNGAE-PATSQCLNVKPITIGKLNP 392

[Close]

BLAST of CmoCh13G003230 vs. Swiss-Prot
Match: PGLR2_ARATH (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 344.4 bits (882), Expect = 1.3e-93
Identity = 169/345 (48.99%), Postives = 220/345 (63.77%), Query Frame = 1


Query: 2   AWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAP------------- 61
           AWK+AC +   S I +P G + V+   FKGPC  P+  +L G  +AP             
Sbjct: 92  AWKEACAAG--STITVPKGEYMVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPHAGWI 151

Query: 62  -------------KRVFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLD 121
                        K +FDGQG  AWK NDC K   C  LPI I             TS +
Sbjct: 152 DFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTN 211

Query: 122 SKNFHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDG 181
           SK FH+N+L C N+T S+I I AP +S NTDGIHIGRSN + +  +KI TGDDC+S+GDG
Sbjct: 212 SKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDG 271

Query: 182 SKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASG 241
           ++ + + NV CGPGHGISIGSLGRY NE+PV+GV V+ C I NT NGVRIKTWP S   G
Sbjct: 272 TENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGS-PPG 331

Query: 242 TATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIK 301
            A+N+ F DI M NVS P+LIDQEYCP+  C   +PS++K+S V+ K I+G+ AT VA+K
Sbjct: 332 IASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSDVTIKGIKGTSATKVAVK 391

Query: 302 LICSRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNP 308
           L+CS+ +PC  + + DI+L +NG +GP  S C+N++PI+SGK  P
Sbjct: 392 LMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVP 433

[Close]

BLAST of CmoCh13G003230 vs. TrEMBL
Match: A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 2.5e-139
Identity = 244/343 (71.14%), Postives = 271/343 (79.01%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           +AW DAC S+R SA+VIP G FKV+EG FKGPC SPI+F++ GTLQAPK           
Sbjct: 52  DAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFA 111

Query: 61  -----------VFDGQGKAAWKKNDCHKRINCAQLPI-------------RITSLDSKNF 120
                      VFDGQGKA W+KNDCHKRI+CA+LPI             RITSLDSKNF
Sbjct: 112 YVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNF 171

Query: 121 HINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 180
           H+N+LGCNNLTF  +NI APE SPNTDGIHIGRS  I+I  S+IATGDDCISLGDGSK+I
Sbjct: 172 HVNILGCNNLTFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRI 231

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTATN 240
            + NV CGPGHGISIGSLG+Y NEEPVEGVIV NCTIINTTNGVRIKTWPAS  +G ATN
Sbjct: 232 KVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATN 291

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 300
           M F DI MVNVSNPILIDQEYCPWNQCNR+IPS IKI+KVSFKNIRGS ATPVA+KLICS
Sbjct: 292 MHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICS 351

Query: 301 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 310
            +LPC+EVKV +IDL YNGIKG ITS+C NV+PIISG QNP I
Sbjct: 352 SNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 394

[Close]

BLAST of CmoCh13G003230 vs. TrEMBL
Match: A0A0D2NBK5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G193800 PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.7e-108
Identity = 192/344 (55.81%), Postives = 234/344 (68.02%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           NAWK+AC S     IVIP G + + E    GPC +PI  QL GTL AP+           
Sbjct: 45  NAWKEACASPEPVNIVIPEGTYLLSEATLNGPCQAPISLQLQGTLMAPEDPTVFKEPTWV 104

Query: 61  --------------VFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLDS 120
                         VFDGQG  AW +NDC K  NC +LPI I             T+ DS
Sbjct: 105 SISYVDSLTMFGGGVFDGQGATAWGQNDCSKNKNCVKLPINIRFHNVKNSLIQDITTKDS 164

Query: 121 KNFHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGS 180
           K FH+NVLGC+N+TF +  ++APE+S NTDGIHIGRS+ + I  SKI TGDDC+SLGDGS
Sbjct: 165 KQFHVNVLGCSNITFQSFTVSAPEESLNTDGIHIGRSDGVYILDSKIETGDDCVSLGDGS 224

Query: 181 KQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGT 240
             + +  VTCGPGHGISIGSLG+Y NEEP+ GV V  CT+ NT NGVRIKTWPAS   G+
Sbjct: 225 NNVKVQGVTCGPGHGISIGSLGKYKNEEPISGVFVTQCTLANTMNGVRIKTWPAS-QPGS 284

Query: 241 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKL 300
           AT++ F DI M NV NPIL+DQEYCPWNQC+  +PS++++SK+SFKNIRG+  T +A+KL
Sbjct: 285 ATDIHFEDITMDNVGNPILVDQEYCPWNQCDLSVPSRVQLSKLSFKNIRGTSKTQIAVKL 344

Query: 301 ICSRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNP 308
           ICS  LPCDEV++ DIDLTYNG +GP  SQC+NV P +SG QNP
Sbjct: 345 ICSSGLPCDEVELADIDLTYNGPEGPAISQCSNVHPALSGVQNP 387

[Close]

BLAST of CmoCh13G003230 vs. TrEMBL
Match: E3VSV7_CUCPE (Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 3.9e-108
Identity = 194/344 (56.40%), Postives = 241/344 (70.06%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           +AWK+AC S   S +++P G +++ E   KGPC SPI+  + GTLQA             
Sbjct: 59  DAWKEACASATPSKVLVPKGTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVM 118

Query: 61  ------------VFDGQGKAAWKKNDCHKRINCAQLPIR-------------ITSLDSKN 120
                       VFDGQGKA W+KNDCH +  C +LP+              ITSLDSKN
Sbjct: 119 EYIDHLTLSGTGVFDGQGKAGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKN 178

Query: 121 FHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQ 180
           FHINVL   NLTF ++ ITAP  SPNTDGIH+G S  + I  + IATGDDCIS+G  +++
Sbjct: 179 FHINVLAGKNLTFDHLKITAPHNSPNTDGIHLGDSEDVYILNTAIATGDDCISVGYTNRK 238

Query: 181 INITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTAT 240
           I I++VTCGPGHGISIGSLG+Y  E+ V GV VK C +I TTNGVRIK+WP SA S  A+
Sbjct: 239 ITISDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPAS 298

Query: 241 NMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLIC 300
           +M F DI M NV+NPI+IDQEYCPWNQCNRK+PS IKISKVSFKNIRG+ +TPVA+KL+C
Sbjct: 299 DMHFEDIEMENVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNIRGTSSTPVAVKLVC 358

Query: 301 SRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 310
           S+  PC+ V++ DIDLTY+G +GP  S+C+NV+P ISGKQNP I
Sbjct: 359 SKSNPCEGVEIADIDLTYSGKEGPAVSECSNVKPTISGKQNPPI 402

[Close]

BLAST of CmoCh13G003230 vs. TrEMBL
Match: W9SBW1_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_020040 PE=3 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 9.5e-107
Identity = 195/345 (56.52%), Postives = 234/345 (67.83%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           NAWKDAC S     ++IP G +K+     +GPC  PI  QL GTL+AP            
Sbjct: 47  NAWKDACASPIRGKVLIPRGTYKLSRATLEGPCKVPIKVQLQGTLRAPTDPSGFNEGDGW 106

Query: 61  ---------------VFDGQGKAAWKKNDCHKRINCAQLPIR-------------ITSLD 120
                           FDGQG A W KN C ++  C +LPI              I SL+
Sbjct: 107 VTFRNVDGLTLAGGGTFDGQGSAVWGKN-CEQQQYCGKLPINFRFDFVTNSLIRDIISLN 166

Query: 121 SKNFHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDG 180
           SK FHINVLG  N+TFS + I APE SPNTDGIHIGRS+ I+IT + I TGDDC+SLGDG
Sbjct: 167 SKQFHINVLGGQNVTFSRVKIIAPEDSPNTDGIHIGRSSGISITDTVIQTGDDCVSLGDG 226

Query: 181 SKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASG 240
            K+I+I  VTCGPGHGIS+GSLG+Y+NE+PVEGVIV+NCTI NT+NGVR+KTWP S   G
Sbjct: 227 CKEISIRRVTCGPGHGISVGSLGKYSNEDPVEGVIVRNCTIRNTSNGVRVKTWPDS-HDG 286

Query: 241 TATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIK 300
            A+N+ F DI+M NV NP+LIDQEYCPWNQCN K+PS+IKIS VSFKNIRG+  T +A+ 
Sbjct: 287 LASNLHFEDIVMENVGNPVLIDQEYCPWNQCNAKVPSRIKISDVSFKNIRGTSTTALAVN 346

Query: 301 LICSRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNP 308
           L+CS   PC  V++ DIDL Y+G  GPI SQC NV+P ISGKQNP
Sbjct: 347 LVCSSGFPCQNVEIADIDLQYSGNDGPIMSQCKNVKPTISGKQNP 389

[Close]

BLAST of CmoCh13G003230 vs. TrEMBL
Match: A0A059DD70_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A01068 PE=3 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 1.6e-106
Identity = 188/346 (54.34%), Postives = 236/346 (68.21%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAP------------ 60
           +AWK+AC +  +S +++P G + ++E + +GPC S I+ Q+ GTLQAP            
Sbjct: 46  SAWKEACAATEASKVLVPAGTYVLREVVLEGPCKSSIEVQVDGTLQAPADPAQMKTDGWV 105

Query: 61  ------------KRVFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLDS 120
                          FDGQGK AW KNDC+ + +C  LPI I             TSLDS
Sbjct: 106 AFRGVDLLTLSGSGTFDGQGKTAWTKNDCNLKTDCVTLPINIRFDSVTNGMVKDITSLDS 165

Query: 121 KNFHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGS 180
           K FH+NVL C NLTF ++ ITAP  SPNTDGIHIGRS  I I  + I TGDDCIS+GDGS
Sbjct: 166 KQFHVNVLNCKNLTFQHVTITAPGDSPNTDGIHIGRSTGINIIDTGIKTGDDCISIGDGS 225

Query: 181 KQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGT 240
           +Q+ IT VTCGPGHGIS+GSLG+Y NE+PV G+ VKNCT+  T NG+RIKTWPAS A GT
Sbjct: 226 EQMTITGVTCGPGHGISVGSLGKYPNEQPVTGLHVKNCTMTGTMNGLRIKTWPASPA-GT 285

Query: 241 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKL 300
           A++M F DI + NV NP++IDQ+YCPWNQC  + PS+IKIS VSF  IRG+ +T  A+ L
Sbjct: 286 ASDMHFEDITLDNVGNPVIIDQQYCPWNQCKSQAPSQIKISNVSFSRIRGTSSTQAAVTL 345

Query: 301 ICSRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 310
            CS  LPC EV++ DID+ Y+G +GP TS C+NV+P ISGKQNP I
Sbjct: 346 ACSSRLPCTEVEISDIDIAYHGPEGPATSVCSNVKPTISGKQNPAI 390

[Close]

BLAST of CmoCh13G003230 vs. TAIR10
Match: AT3G07820.1 (AT3G07820.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 365.2 bits (936), Expect = 4.1e-101
Identity = 176/343 (51.31%), Postives = 228/343 (66.47%), Query Frame = 1


Query: 2   AWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKRV---------- 61
           A+  AC S+  S +VIP G FK+ E   +GPC +PI+  L GT++A              
Sbjct: 42  AFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQGTVKADGNAIQGKEKWVVF 101

Query: 62  -------------FDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLDSKN 121
                        FDG+G AAW+ N+CHK   C +LPI I             +S+D+KN
Sbjct: 102 GNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIRDISSIDAKN 161

Query: 122 FHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQ 181
           FHINVLG  N+T +NI I APE SPNTDGIH+GRS+ + I  S I+TGDDCIS+GDG K 
Sbjct: 162 FHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDCISVGDGMKN 221

Query: 182 INITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTAT 241
           +++  VTCGPGHGIS+GSLGRY +E+ V G+ V NCT+  T NG+RIKTWP++A S TA+
Sbjct: 222 LHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACSTTAS 281

Query: 242 NMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLIC 301
           ++ F DII+ +VSNPILIDQEYCPWNQCN++  S IK+  +SFKNIRG+     A+KL+C
Sbjct: 282 DIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSGNKDAVKLLC 341

Query: 302 SRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPK 309
           S+  PC  V++GDID+ YNG  GP T  C+NV P I G Q+PK
Sbjct: 342 SKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQSPK 384

[Close]

BLAST of CmoCh13G003230 vs. TAIR10
Match: AT3G07840.1 (AT3G07840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 361.3 bits (926), Expect = 5.9e-100
Identity = 172/343 (50.15%), Postives = 227/343 (66.18%), Query Frame = 1


Query: 2   AWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR----------- 61
           A+  AC +  +S +VI  G FK+ E    GPC +P++  L GTL+A  +           
Sbjct: 43  AFTSACQAPTASKVVITKGEFKLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVF 102

Query: 62  ------------VFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLDSKN 121
                       +FDG+G AAW+ N+CHK   C +LPI I             +S+D+KN
Sbjct: 103 LRINGFKLNGGGIFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKN 162

Query: 122 FHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQ 181
           FHINVLG  N+TF N+ + AP +SPNTDGIH+GRS  + I  SKIATGDDCIS+GDG K 
Sbjct: 163 FHINVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKN 222

Query: 182 INITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTAT 241
           +++ NV CGPGHGIS+GSLGRY +E+ V G+ V NCT+  T NG+RIKTWP++A + TA+
Sbjct: 223 LHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTAS 282

Query: 242 NMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLIC 301
            + F +II+ NVSNPILIDQEYCPWNQCN++ PS IK+  +SFKNIRG+     A+KL+C
Sbjct: 283 GIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLC 342

Query: 302 SRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPK 309
           S+  PC  V++G+I+L Y G  GP T  C+NV P + G QNPK
Sbjct: 343 SKAHPCANVEIGNINLEYKGADGPPTFMCSNVSPKLVGTQNPK 385

[Close]

BLAST of CmoCh13G003230 vs. TAIR10
Match: AT5G48140.1 (AT5G48140.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 357.5 bits (916), Expect = 8.6e-99
Identity = 170/342 (49.71%), Postives = 228/342 (66.67%), Query Frame = 1


Query: 2   AWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR----------- 61
           A+ +AC     S ++IP G +K+ E +  GPC +PI   L GT++A              
Sbjct: 43  AFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAPIRIALLGTVKADGNANGKEKWVAFR 102

Query: 62  -----------VFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLDSKNF 121
                      VFDG+G AAW+ N+CHK  NC +LPI I             TSLD+K+F
Sbjct: 103 NINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVTDAKIRGITSLDAKHF 162

Query: 122 HINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 181
           HINV+G  N+TF ++ I AP +SPNTDGIH+GRS+ I I  S I+TGDDC+S+GDG K +
Sbjct: 163 HINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFISTGDDCVSVGDGMKNL 222

Query: 182 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTATN 241
            +  VTCGPGHGISIGSLGRY++EE V G+ + NCT+  T NG+RIKTWP++A + TA++
Sbjct: 223 LVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTWPSAACTTTASD 282

Query: 242 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 301
           + F +I++ NVSNPILIDQEYCPWNQCN++ PS IK++ +SFK IRG+     A+KL+CS
Sbjct: 283 IHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTSGNKDAVKLLCS 342

Query: 302 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPK 309
           +  PC  V+VGD+++ Y G  GP T QC+NV P + G Q PK
Sbjct: 343 KGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPK 384

[Close]

BLAST of CmoCh13G003230 vs. TAIR10
Match: AT3G07830.1 (AT3G07830.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 353.6 bits (906), Expect = 1.2e-97
Identity = 169/343 (49.27%), Postives = 224/343 (65.31%), Query Frame = 1


Query: 2   AWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR----------- 61
           A+  AC S     +VIP G FK+ E +  GPC SP++  L GT+ A              
Sbjct: 43  AFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPVEITLLGTVLADGNSIHGKEKWVVF 102

Query: 62  ------------VFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLDSKN 121
                        FDG+G AAW+ N+CHK   C +LPI I             +S+D+KN
Sbjct: 103 QRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVTNAEIRDISSIDAKN 162

Query: 122 FHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQ 181
           FHINV+G  N+TF N+ I AP +SPNTDGIH+GRS  ++I  S+I+TGDDC+S+GDG   
Sbjct: 163 FHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDCVSVGDGMVN 222

Query: 182 INITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTAT 241
           + + NV CGPGHGIS+GSLGRY +E+ V G+ V NCT+  T NG+RIKTWP++A S TA+
Sbjct: 223 LLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWPSAACSTTAS 282

Query: 242 NMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLIC 301
           N+ F +II+ NVSNPILIDQEYCPWNQCN++  S IK++ +SF+ IRG+     A+KL+C
Sbjct: 283 NIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSIKLANISFRRIRGTSGNKDAVKLLC 342

Query: 302 SRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPK 309
           S+  PC+ V+VGDI++ Y G  GP T  C+NVRP + G Q PK
Sbjct: 343 SKGYPCENVQVGDINIQYTGADGPATFMCSNVRPKLVGTQFPK 385

[Close]

BLAST of CmoCh13G003230 vs. TAIR10
Match: AT3G14040.1 (AT3G14040.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 346.7 bits (888), Expect = 1.5e-95
Identity = 170/345 (49.28%), Postives = 221/345 (64.06%), Query Frame = 1


Query: 2   AWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAP------------- 61
           AWK+AC +   S I +P G + V+   FKGPC  P+  +L G  +AP             
Sbjct: 93  AWKEACAAG--STITVPKGEYLVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPHAGWI 152

Query: 62  -------------KRVFDGQGKAAWKKNDCHKRINCAQLPIRI-------------TSLD 121
                        K +FDGQG  AWK NDC K   C  LPI I             TS +
Sbjct: 153 DFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTN 212

Query: 122 SKNFHINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDG 181
           SK FH+N+L C N+T ++I I AP +S NTDGIHIGRSN + +  +KI TGDDC+S+GDG
Sbjct: 213 SKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDG 272

Query: 182 SKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASG 241
           ++ + + NV CGPGHGISIGSLGRY NE+PV+GV V+ C I NT NGVRIKTWP S   G
Sbjct: 273 TENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGS-PPG 332

Query: 242 TATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIK 301
            A+N+ F DI M NVS P+LIDQEYCP+  C   +PSK+K+S V+ KNI+G+ AT VA+K
Sbjct: 333 IASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIKNIKGTSATKVAVK 392

Query: 302 LICSRHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNP 308
           L+CS+ +PC  + + DI+L +NG +GP  S C+N++PI+SGK  P
Sbjct: 393 LMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVP 434

[Close]

BLAST of CmoCh13G003230 vs. NCBI nr
Match: gi|659111053|ref|XP_008455553.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 508.8 bits (1309), Expect = 6.5e-141
Identity = 246/343 (71.72%), Postives = 274/343 (79.88%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           +AW DAC S R SA+VIP G FKV+EG FKGPC SP++ ++ GTLQAPK           
Sbjct: 53  DAWNDACASLRPSAVVIPKGTFKVREGEFKGPCKSPVEVRVRGTLQAPKHPHGDSWITFA 112

Query: 61  -----------VFDGQGKAAWKKNDCHKRINCAQLPI-------------RITSLDSKNF 120
                      VFDGQGKA W+KNDCHK I+CA+LPI             RITSLDSKNF
Sbjct: 113 YVDRMTLSGGGVFDGQGKAGWEKNDCHKHISCARLPISLRFNFITNSIVRRITSLDSKNF 172

Query: 121 HINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 180
           H+N+LGCNNLTF  +NI APE SPNTDGIHIGRS  I+I  S+IATGDDCISLGDGSK++
Sbjct: 173 HVNILGCNNLTFDGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRV 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTATN 240
            ITNVTCGPGHGISIGSLG+YTNEEPVEGVIVKNCTI+NTTNGVRIKTWPAS  +G ATN
Sbjct: 233 KITNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNGVRIKTWPASPVAGIATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 300
           M FLDI MVNVSNPILIDQEYCPWNQCNRKIPSKIKI+KVSFKNIRGS ATPVA+KLICS
Sbjct: 293 MHFLDITMVNVSNPILIDQEYCPWNQCNRKIPSKIKINKVSFKNIRGSSATPVAVKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 310
            +LPC+EVKV +IDL YNGIKG +TS+C NV+PIISG QNP I
Sbjct: 353 SNLPCEEVKVANIDLVYNGIKGSLTSECMNVKPIISGTQNPPI 395

[Close]

BLAST of CmoCh13G003230 vs. NCBI nr
Match: gi|778730103|ref|XP_011659699.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 503.1 bits (1294), Expect = 3.6e-139
Identity = 244/343 (71.14%), Postives = 271/343 (79.01%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           +AW DAC S+R SA+VIP G FKV+EG FKGPC SPI+F++ GTLQAPK           
Sbjct: 37  DAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFA 96

Query: 61  -----------VFDGQGKAAWKKNDCHKRINCAQLPI-------------RITSLDSKNF 120
                      VFDGQGKA W+KNDCHKRI+CA+LPI             RITSLDSKNF
Sbjct: 97  YVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNF 156

Query: 121 HINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 180
           H+N+LGCNNLTF  +NI APE SPNTDGIHIGRS  I+I  S+IATGDDCISLGDGSK+I
Sbjct: 157 HVNILGCNNLTFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRI 216

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTATN 240
            + NV CGPGHGISIGSLG+Y NEEPVEGVIV NCTIINTTNGVRIKTWPAS  +G ATN
Sbjct: 217 KVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATN 276

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 300
           M F DI MVNVSNPILIDQEYCPWNQCNR+IPS IKI+KVSFKNIRGS ATPVA+KLICS
Sbjct: 277 MHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICS 336

Query: 301 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 310
            +LPC+EVKV +IDL YNGIKG ITS+C NV+PIISG QNP I
Sbjct: 337 SNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 379

[Close]

BLAST of CmoCh13G003230 vs. NCBI nr
Match: gi|700188231|gb|KGN43464.1| (hypothetical protein Csa_7G037910 [Cucumis sativus])

HSP 1 Score: 503.1 bits (1294), Expect = 3.6e-139
Identity = 244/343 (71.14%), Postives = 271/343 (79.01%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           +AW DAC S+R SA+VIP G FKV+EG FKGPC SPI+F++ GTLQAPK           
Sbjct: 52  DAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFA 111

Query: 61  -----------VFDGQGKAAWKKNDCHKRINCAQLPI-------------RITSLDSKNF 120
                      VFDGQGKA W+KNDCHKRI+CA+LPI             RITSLDSKNF
Sbjct: 112 YVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNF 171

Query: 121 HINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 180
           H+N+LGCNNLTF  +NI APE SPNTDGIHIGRS  I+I  S+IATGDDCISLGDGSK+I
Sbjct: 172 HVNILGCNNLTFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRI 231

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTATN 240
            + NV CGPGHGISIGSLG+Y NEEPVEGVIV NCTIINTTNGVRIKTWPAS  +G ATN
Sbjct: 232 KVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATN 291

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 300
           M F DI MVNVSNPILIDQEYCPWNQCNR+IPS IKI+KVSFKNIRGS ATPVA+KLICS
Sbjct: 292 MHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICS 351

Query: 301 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 310
            +LPC+EVKV +IDL YNGIKG ITS+C NV+PIISG QNP I
Sbjct: 352 SNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 394

[Close]

BLAST of CmoCh13G003230 vs. NCBI nr
Match: gi|449445367|ref|XP_004140444.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 431.0 bits (1107), Expect = 1.7e-117
Identity = 208/343 (60.64%), Postives = 248/343 (72.30%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKR---------- 60
           NAWK AC S   S ++IP GI+ + +   KGPC S I+ Q+ GTLQAP            
Sbjct: 46  NAWKGACASTSRSKVLIPKGIYLLSQSNLKGPCKSSIEIQVQGTLQAPPDPKGDGLVILE 105

Query: 61  -----------VFDGQGKAAWKKNDCHKRINCAQLPIR-------------ITSLDSKNF 120
                      VFDGQGKA W+KNDCHK+  C +LP+              ITSLDSKNF
Sbjct: 106 YIDQLTLSGTGVFDGQGKAGWEKNDCHKKKICTKLPMTLKFNFVTNSIVKDITSLDSKNF 165

Query: 121 HINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 180
           HIN+LGC N+TF ++ I+APE SPNTDGIHI  S +I I  +KI+TGDDC+S+GD +K I
Sbjct: 166 HINLLGCKNITFEHVTISAPENSPNTDGIHISSSEQIRILNTKISTGDDCVSVGDSNKDI 225

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTATN 240
            I +VTCGPGHGISIGSLG+YT E+ VEGV V  C +I TTNGVRIKTWP SA   +A++
Sbjct: 226 TIRDVTCGPGHGISIGSLGKYTKEKAVEGVWVTKCKLIKTTNGVRIKTWPDSAVKYSASD 285

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 300
           M F DI M++VSNPILIDQEYCPWNQCNRKIPS IKISKVSFKNIRG+  TPVA+KL+CS
Sbjct: 286 MHFEDIEMIDVSNPILIDQEYCPWNQCNRKIPSSIKISKVSFKNIRGTTVTPVAVKLVCS 345

Query: 301 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 310
           + +PC+ V+V DIDL YNG KG ITSQC NV+PIISGKQNP+I
Sbjct: 346 KSIPCEAVEVADIDLKYNGNKGSITSQCANVKPIISGKQNPRI 388

[Close]

BLAST of CmoCh13G003230 vs. NCBI nr
Match: gi|659120497|ref|XP_008460220.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 427.2 bits (1097), Expect = 2.5e-116
Identity = 206/343 (60.06%), Postives = 247/343 (72.01%), Query Frame = 1


Query: 1   NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPK----------- 60
           NAWK AC S   S ++IP G++ + +   KGPC S I+ Q+ GTLQAP            
Sbjct: 49  NAWKGACASTSRSKVLIPKGVYLLSQSNLKGPCKSSIEIQVDGTLQAPPDPKGDGLVILE 108

Query: 61  ----------RVFDGQGKAAWKKNDCHKRINCAQLPIR-------------ITSLDSKNF 120
                      VFDGQGK  W+KNDCHK+  C +LP+              ITSLDSKNF
Sbjct: 109 YIDKLTLSGTGVFDGQGKVGWEKNDCHKKKICTKLPMTLKFNFVTNSIVRDITSLDSKNF 168

Query: 121 HINVLGCNNLTFSNINITAPEKSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 180
           HIN+LGC NLTF ++ I+APE SPNTDGIHI  S +I I  +KI+TGDDC+S+GD +KQI
Sbjct: 169 HINILGCKNLTFQHVTISAPEDSPNTDGIHISSSEQITILNTKISTGDDCVSVGDSNKQI 228

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVKNCTIINTTNGVRIKTWPASAASGTATN 240
            I +VTCGPGHGISIGSLG+Y+ E+ VEGV V  C + +TTNGVRIKTWP SA   +A++
Sbjct: 229 TIRDVTCGPGHGISIGSLGKYSKEKAVEGVWVTKCKLTSTTNGVRIKTWPDSAGEYSASD 288

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 300
           M F DI M NVSNPI+IDQEYCPWNQCN KIPS IKISKVSFKNIRG+ ATPVA+KL+CS
Sbjct: 289 MHFEDIEMNNVSNPIIIDQEYCPWNQCNPKIPSSIKISKVSFKNIRGTSATPVAVKLVCS 348

Query: 301 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 310
           + +PC+ V+V DIDL YNG KGPI SQC NV+PIISGKQNP+I
Sbjct: 349 KKIPCEAVEVVDIDLKYNGNKGPIKSQCANVKPIISGKQNPRI 391

The following BLAST results are available for this feature:

BLAST of CmoCh13G003230 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGLR_OENOR	5.0e-101	53.76	Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1	[more]
PGLR_BRANA	5.8e-97	50.44	Polygalacturonase OS=Brassica napus PE=2 SV=1	[more]
PGLR_GOSHI	5.4e-95	49.71	Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1	[more]
PGLR_GOSBA	2.7e-94	49.42	Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1	[more]
PGLR2_ARATH	1.3e-93	48.99	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=...	[more]

BLAST of CmoCh13G003230 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3B7_CUCSA	2.5e-139	71.14	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1	[more]
A0A0D2NBK5_GOSRA	1.7e-108	55.81	Uncharacterized protein OS=Gossypium raimondii GN=B456_001G193800 PE=3 SV=1	[more]
E3VSV7_CUCPE	3.9e-108	56.40	Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1	[more]
W9SBW1_9ROSA	9.5e-107	56.52	Uncharacterized protein OS=Morus notabilis GN=L484_020040 PE=3 SV=1	[more]
A0A059DD70_EUCGR	1.6e-106	54.34	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A01068 PE=3 SV=1	[more]

BLAST of CmoCh13G003230 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G07820.1	4.1e-101	51.31	Pectin lyase-like superfamily protein	[more]
AT3G07840.1	5.9e-100	50.15	Pectin lyase-like superfamily protein	[more]
AT5G48140.1	8.6e-99	49.71	Pectin lyase-like superfamily protein	[more]
AT3G07830.1	1.2e-97	49.27	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	1.5e-95	49.28	Pectin lyase-like superfamily protein	[more]

BLAST of CmoCh13G003230 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659111053\|ref\|XP_008455553.1\|	6.5e-141	71.72	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
gi\|778730103\|ref\|XP_011659699.1\|	3.6e-139	71.14	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]
gi\|700188231\|gb\|KGN43464.1\|	3.6e-139	71.14	hypothetical protein Csa_7G037910 [Cucumis sativus]	[more]
gi\|449445367\|ref\|XP_004140444.1\|	1.7e-117	60.64	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]
gi\|659120497\|ref\|XP_008460220.1\|	2.5e-116	60.06	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000743	Glyco_hydro_28
IPR006626	PbH1
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071555	cell wall organization
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh13G003230.1	CmoCh13G003230.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 50..298 score: 1.4
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 150..163 scor
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 93..119 score: 49.0coord: 173..194 score: 0.44coord: 143..163 score: 56
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 1..307 score: 1.07
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 1..308 score: 4.1
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..309 score: 3.6E
None	No IPR available	PANTHER	PTHR31375:SF37	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 1..309 score: 3.6E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh13G003230	CmaCh18G005950	Cucurbita maxima (Rimu)	cmacmoB395

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh13G003230	Cucumber (Chinese Long) v3	cmocucB0221
CmoCh13G003230	Watermelon (97103) v2	cmowmbB198
CmoCh13G003230	Wax gourd	cmowgoB0259
CmoCh13G003230	Cucurbita moschata (Rifu)	cmocmoB135
CmoCh13G003230	Cucumber (Gy14) v1	cgycmoB0594
CmoCh13G003230	Cucurbita maxima (Rimu)	cmacmoB218
CmoCh13G003230	Wild cucumber (PI 183967)	cmocpiB192
CmoCh13G003230	Cucumber (Chinese Long) v2	cmocuB188
CmoCh13G003230	Watermelon (Charleston Gray)	cmowcgB179
CmoCh13G003230	Cucurbita pepo (Zucchini)	cmocpeB204
CmoCh13G003230	Cucumber (Gy14) v2	cgybcmoB057
CmoCh13G003230	Melon (DHL92) v3.6.1	cmomedB203