CmoCh07G002050 (gene) Cucurbita moschata (Rifu)

NameCmoCh07G002050
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionGDSL esterase/lipase
LocationCmo_Chr07 : 1039740 .. 1041465 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAACGGCGAGCTTTCTTGATTCTTGAAGATGGAAATTATGGGGACGAGGGTTAAGAATGCCGTGCTATTGTTATTCATAATGGGGATTGGTTTTTGTCCGCCATTAATGGAGGGGAAGGAGATTGCAGAGGTGAATGTGCAGCAGTTGAGGAAATTGGCGTCCAAGTATAATGTGACGAGCTTGTTGGTGTTTGGGGATTCTAGCGTTGATCCTGGGAACAACAATGTTCTTTCCACGAACGTCAAGAGTAATTTCCCTCCTTACGGGAAGGACTTCTTTAATGCTCGCCCAACTGGGAGATTCTGCGATGGAAGGCTCGCCACCGACTTCATTGGTAATTAACCACTTTTTCTTCTTAAAATCGTACGTTCAAGTTTCAATTTTTATTGAACCCAGCTTTTGAAATGATGATTTTAATGGACATTACAGCTGAGGCGATGGGATTTGGAGAAACAGTGCCAGCATTTCTGGACAGAACTTTGAAGCCAATCGATCTCTTGCATGGCGTTAGCTTTGCTTCTGCTTCTTCAGGCTACGATGATCTCACCGCCAACTTATCTGTAAACAAAACAAAAGCACCATCTTTTCTCTGTTCTTAATTTCGTTTAACAACCTTGTACGGTTTTCGACTAACTTATAAAGCTCTGTTCAGAAAGTGCTGTCGCTTCCGAAGCAGTTGGAGTACTTCATGCATTACAAAATCCATTTATCCCGACTGGTCGGTTACGACAAAGCAGAGAACATAATCAGAAACGCCATTGTTGTCCTCAGCATGGGCACAAACGACTTCCTCGAAAACTACTTCTTGGAGCCCCAACGCCGCAAGCAATTCAGCTTGGATCAATACCAGAATTTCTTGGTGTCTTCCATGTCTCATAACGTCGAGGTCTCTCTCCTCTGTCCTTGACAGTATTTAATGTTTTGTTGATGTTCTTAACCATGGTGAGTTGCAGGTGATGCATAGATTAGGAATTAGACGGCTAGTGGTTGTGGGAGTACCGCCATTGGGGTGTATGCCAGTTGTGAGAACCATAACCAACCAGATCACGACATGTTCCACATCCTTCAACAGAGCAGCCTACGCATTCAATGCAAAGATCAAGCTGAAATTGGCAGCTATGGAAGCACACTTGGGGTTGCTCACTAGCTTTGTCGATGCTTATGCCATTGTTCAAGACGCTGTCGATAACCCCACCGCCTACGGTACTCAAACAATACTCAATTTTTGTGTTCCCTTCCAATTTTTGTATGGCCTCAAACTAGAAGGTGTGTGTGTTTGGAAATGTGGGCACAGGTTTAAGGGAAACAGCGAAGGGGTGCTGCGGGACGGGATTGGTTGAATTTGGCGAGACATGCAAAGGCTCAACCACGTGCAGTGACCCACAAAGCTACGTGTTTTGGGACGCTGTTCATCCCTCCCAGAACATGTACAAAATCTTAGCCGCCAAAGCCATTCAATCTGTTGCACAAGACATTCTATCCTAGTTTTCTTCCATTCTTTTGCATATCCGTAGTTTGAAACTTGATACCCATCTGCTTATTCAACGCGCCCAATGTATGGATTCAGTTCACCAATACAATGTCAAAGTCAGTCTGCTGGTACACAGGAAATGTTCAATTCCTTCAACAAATTACATAACTTGTTTTGCTTCCTTAATTTATACATTGAACTCAAAACTCAAAATTCCTTTTCTTTATTATATCTGTAATAATCATATTCC

mRNA sequence

AAAAAAACGGCGAGCTTTCTTGATTCTTGAAGATGGAAATTATGGGGACGAGGGTTAAGAATGCCGTGCTATTGTTATTCATAATGGGGATTGGTTTTTGTCCGCCATTAATGGAGGGGAAGGAGATTGCAGAGGTGAATGTGCAGCAGTTGAGGAAATTGGCGTCCAAGTATAATGTGACGAGCTTGTTGGTGTTTGGGGATTCTAGCGTTGATCCTGGGAACAACAATGTTCTTTCCACGAACGTCAAGAGTAATTTCCCTCCTTACGGGAAGGACTTCTTTAATGCTCGCCCAACTGGGAGATTCTGCGATGGAAGGCTCGCCACCGACTTCATTGCTGAGGCGATGGGATTTGGAGAAACAGTGCCAGCATTTCTGGACAGAACTTTGAAGCCAATCGATCTCTTGCATGGCGTTAGCTTTGCTTCTGCTTCTTCAGGCTACGATGATCTCACCGCCAACTTATCTAAAGTGCTGTCGCTTCCGAAGCAGTTGGAGTACTTCATGCATTACAAAATCCATTTATCCCGACTGGTCGGTTACGACAAAGCAGAGAACATAATCAGAAACGCCATTGTTGTCCTCAGCATGGGCACAAACGACTTCCTCGAAAACTACTTCTTGGAGCCCCAACGCCGCAAGCAATTCAGCTTGGATCAATACCAGAATTTCTTGGTGTCTTCCATGTCTCATAACGTCGAGGTGATGCATAGATTAGGAATTAGACGGCTAGTGGTTGTGGGAGTACCGCCATTGGGGTGTATGCCAGTTGTGAGAACCATAACCAACCAGATCACGACATGTTCCACATCCTTCAACAGAGCAGCCTACGCATTCAATGCAAAGATCAAGCTGAAATTGGCAGCTATGGAAGCACACTTGGGGTTGCTCACTAGCTTTGTCGATGCTTATGCCATTGTTCAAGACGCTGTCGATAACCCCACCGCCTACGGTTTAAGGGAAACAGCGAAGGGGTGCTGCGGGACGGGATTGGTTGAATTTGGCGAGACATGCAAAGGCTCAACCACGTGCAGTGACCCACAAAGCTACGTGTTTTGGGACGCTGTTCATCCCTCCCAGAACATGTACAAAATCTTAGCCGCCAAAGCCATTCAATCTGTTGCACAAGACATTCTATCCTAGTTTTCTTCCATTCTTTTGCATATCCGTAGTTTGAAACTTGATACCCATCTGCTTATTCAACGCGCCCAATGTATGGATTCAGTTCACCAATACAATGTCAAAGTCAGTCTGCTGGTACACAGGAAATGTTCAATTCCTTCAACAAATTACATAACTTGTTTTGCTTCCTTAATTTATACATTGAACTCAAAACTCAAAATTCCTTTTCTTTATTATATCTGTAATAATCATATTCC

Coding sequence (CDS)

ATGGAAATTATGGGGACGAGGGTTAAGAATGCCGTGCTATTGTTATTCATAATGGGGATTGGTTTTTGTCCGCCATTAATGGAGGGGAAGGAGATTGCAGAGGTGAATGTGCAGCAGTTGAGGAAATTGGCGTCCAAGTATAATGTGACGAGCTTGTTGGTGTTTGGGGATTCTAGCGTTGATCCTGGGAACAACAATGTTCTTTCCACGAACGTCAAGAGTAATTTCCCTCCTTACGGGAAGGACTTCTTTAATGCTCGCCCAACTGGGAGATTCTGCGATGGAAGGCTCGCCACCGACTTCATTGCTGAGGCGATGGGATTTGGAGAAACAGTGCCAGCATTTCTGGACAGAACTTTGAAGCCAATCGATCTCTTGCATGGCGTTAGCTTTGCTTCTGCTTCTTCAGGCTACGATGATCTCACCGCCAACTTATCTAAAGTGCTGTCGCTTCCGAAGCAGTTGGAGTACTTCATGCATTACAAAATCCATTTATCCCGACTGGTCGGTTACGACAAAGCAGAGAACATAATCAGAAACGCCATTGTTGTCCTCAGCATGGGCACAAACGACTTCCTCGAAAACTACTTCTTGGAGCCCCAACGCCGCAAGCAATTCAGCTTGGATCAATACCAGAATTTCTTGGTGTCTTCCATGTCTCATAACGTCGAGGTGATGCATAGATTAGGAATTAGACGGCTAGTGGTTGTGGGAGTACCGCCATTGGGGTGTATGCCAGTTGTGAGAACCATAACCAACCAGATCACGACATGTTCCACATCCTTCAACAGAGCAGCCTACGCATTCAATGCAAAGATCAAGCTGAAATTGGCAGCTATGGAAGCACACTTGGGGTTGCTCACTAGCTTTGTCGATGCTTATGCCATTGTTCAAGACGCTGTCGATAACCCCACCGCCTACGGTTTAAGGGAAACAGCGAAGGGGTGCTGCGGGACGGGATTGGTTGAATTTGGCGAGACATGCAAAGGCTCAACCACGTGCAGTGACCCACAAAGCTACGTGTTTTGGGACGCTGTTCATCCCTCCCAGAACATGTACAAAATCTTAGCCGCCAAAGCCATTCAATCTGTTGCACAAGACATTCTATCCTAG
BLAST of CmoCh07G002050 vs. Swiss-Prot
Match: GDL85_ARATH (GDSL esterase/lipase At5g45950 OS=Arabidopsis thaliana GN=At5g45950 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.3e-107
Identity = 184/338 (54.44%), Postives = 250/338 (73.96%), Query Frame = 1

Query: 32  IAEVNVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGR 91
           +  ++V  LR+LA+K+NVTS+LVFGDSSVDPGNNN + T +K NFPPYG++F N +PTGR
Sbjct: 21  VLSLDVHLLRQLAAKHNVTSILVFGDSSVDPGNNNFIKTEMKGNFPPYGENFINHKPTGR 80

Query: 92  FCDGRLATDFIAEAMGFGETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSL 151
            CDG LA D+IAEAMG+   +PAFLD +L   DL  G SFASA SGYDDLTAN+S V S 
Sbjct: 81  LCDGLLAPDYIAEAMGY-PPIPAFLDPSLTQADLTRGASFASAGSGYDDLTANISNVWSF 140

Query: 152 PKQLEYFMHYKIHLSRLVGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQY 211
             Q  YF+HYKIHL++LVG  ++  +I NAI ++SMG+NDFL+NY ++  R+KQF+++QY
Sbjct: 141 TTQANYFLHYKIHLTKLVGPLESAKMINNAIFLMSMGSNDFLQNYLVDFTRQKQFTVEQY 200

Query: 212 QNFLVSSMSHNVEVMHRLGIRRLVVVGVPPLGCMPVVRTITNQITTCSTSFNRAAYAFNA 271
             FL   M ++ +++HRLG +RLVVVGVPP+GCMP+++ +  Q  TC    N+ A++FNA
Sbjct: 201 IEFLSHRMLYDAKMLHRLGAKRLVVVGVPPMGCMPLIKYLRGQ-KTCVDQLNQIAFSFNA 260

Query: 272 KIKLKLAAMEAHLGLLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGS 331
           KI   L  +++ +GL T +VDAY+ +Q+A+ NP  +G  E + GCCGTG  E+GETCK  
Sbjct: 261 KIIKNLELLQSKIGLKTIYVDAYSTIQEAIKNPRKFGFVEASLGCCGTGTYEYGETCKDM 320

Query: 332 TTCSDPQSYVFWDAVHPSQNMYKILAAKAIQSVAQDIL 370
             C DP  YVFWDAVHP+Q MY+I+  KAI S++++ L
Sbjct: 321 QVCKDPTKYVFWDAVHPTQRMYQIIVKKAIASISEEFL 356

BLAST of CmoCh07G002050 vs. Swiss-Prot
Match: GDL48_ARATH (GDSL esterase/lipase At2g42990 OS=Arabidopsis thaliana GN=At2g42990 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 9.1e-73
Identity = 138/313 (44.09%), Postives = 202/313 (64.54%), Query Frame = 1

Query: 49  VTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGF 108
           + +++VFGDSSVD GNNN +ST  ++NF PYG+DF   R TGRFC+GRL++DF +EA G 
Sbjct: 26  IPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGGRATGRFCNGRLSSDFTSEAYGL 85

Query: 109 GETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRL 168
             TVPA+LD +    D   GV FASA +GYD+ TA++  V+ L K++EYF  Y+ +LS  
Sbjct: 86  KPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVLGVIPLWKEVEYFKEYQSNLSAY 145

Query: 169 VGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHR 228
           +G+ +A  IIR ++ ++S+GTNDFLENY+  P RR QFS+ QYQ+FLV      ++ ++R
Sbjct: 146 LGHRRAAKIIRESLYIVSIGTNDFLENYYTLPDRRSQFSISQYQDFLVEIAEVFLKDIYR 205

Query: 229 LGIRRLVVVGVPPLGCMPVVR-TITNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHL-GL 288
           LG R++   G+ P+GC+P+ R T  +   +C+ S+N  A  FN +++  +  +   L G+
Sbjct: 206 LGARKMSFTGISPMGCLPLERVTNLDDPFSCARSYNDLAVDFNGRLRRLVTKLNRELTGI 265

Query: 289 LTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETC--KGSTTCSDPQSYVFWD 348
              F + Y I+ D V  P  YGL  ++  CCGTGL E G  C      TCSD   +VFWD
Sbjct: 266 KIYFANPYDIMWDIVTKPNLYGLEISSSACCGTGLFEMGFLCGQDNPLTCSDANKFVFWD 325

Query: 349 AVHPSQNMYKILA 358
           A HP++   +I++
Sbjct: 326 AFHPTERTNQIVS 338

BLAST of CmoCh07G002050 vs. Swiss-Prot
Match: GDL86_ARATH (GDSL esterase/lipase At5g45960 OS=Arabidopsis thaliana GN=At5g45960 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.0e-72
Identity = 140/318 (44.03%), Postives = 200/318 (62.89%), Query Frame = 1

Query: 46  KYNVTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEA 105
           K++V+++LVFGDS+VDPGNNN + T  K NFPPYG DF N  PTGRFC+GRL TDFIA  
Sbjct: 42  KHSVSAILVFGDSTVDPGNNNYIDTVFKCNFPPYGLDFRNKTPTGRFCNGRLVTDFIASY 101

Query: 106 MGFGETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHL 165
           +G  E VP +LD  L   +L+ GVSFASA SGYD LT  ++ V+ +P QLEYF  YK  L
Sbjct: 102 IGVKENVPPYLDPNLGINELISGVSFASAGSGYDPLTPTITNVIDIPTQLEYFREYKRKL 161

Query: 166 SRLVGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEV 225
              +G  + E  I  A+  +S GTNDF+ NYF  P RRK F+++ YQ F++S++   ++ 
Sbjct: 162 EGKMGKQEMEKHIEEAMFCVSAGTNDFVINYFTIPIRRKTFTIEAYQQFVISNLKQFIQG 221

Query: 226 MHRLGIRRLVVVGVPPLGCMPVVRT------ITNQITTCSTSFNRAAYAFNAKIKLKLAA 285
           + + G R++ V G+PP+GC+P+V T      +TN+   C   F+  A  +N  ++ +LA 
Sbjct: 222 LWKEGARKITVAGLPPIGCLPIVITLFSGEALTNR--RCIDRFSTVATNYNFLLQKQLAL 281

Query: 286 ME---AHLGLLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCK-GSTTCS 345
           M+   AHLG    ++D Y  V + + +P  +G  E   GCCG+G +E    C   S  C 
Sbjct: 282 MQVGLAHLGSKIFYLDVYNPVYEVIRDPRKFGFEEVFSGCCGSGYLEASFLCNPKSYVCP 341

Query: 346 DPQSYVFWDAVHPSQNMY 354
           +  +YVF+D++HPS+  Y
Sbjct: 342 NTSAYVFFDSIHPSEKTY 357

BLAST of CmoCh07G002050 vs. Swiss-Prot
Match: GDL1_ARATH (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 268.1 bits (684), Expect = 1.5e-70
Identity = 132/319 (41.38%), Postives = 201/319 (63.01%), Query Frame = 1

Query: 51  SLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGE 110
           ++LVFGDS++D GNNN + T +++NFPPYG +F     TGRF +G+L  DFIA  MG  +
Sbjct: 37  AILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGIKD 96

Query: 111 TVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVG 170
           TVP FLD  L   D++ GV FASA SGYD+LT   +  LS+ KQ +    Y   LS++VG
Sbjct: 97  TVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQIVG 156

Query: 171 YDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLG 230
            +KA +I+  A+V++S GTNDF  N +  P RR++  +D YQ+F++S++ + V+ ++ +G
Sbjct: 157 DEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYDIG 216

Query: 231 IRRLVVVGVPPLGCMPVVRTITNQITT---CSTSFNRAAYAFNAKIKLKLAAMEAHL-GL 290
            R+++V+G+PP+GC+P+  T+  Q      C    N  +  FN K+K  L  M+++L G 
Sbjct: 217 CRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLTGS 276

Query: 291 LTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGST-TCSDPQSYVFWDA 350
           +  + D Y  + D   NP  YGL+ET +GCCGTG +E    C   T  C +P  Y+FWD 
Sbjct: 277 VIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYLFWDD 336

Query: 351 VHPSQNMYKILAAKAIQSV 365
           +HPSQ  Y +++   ++ +
Sbjct: 337 IHPSQIAYIVISLSLVEQI 355

BLAST of CmoCh07G002050 vs. Swiss-Prot
Match: GDL34_ARATH (GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana GN=At2g04570 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 4.4e-67
Identity = 131/320 (40.94%), Postives = 194/320 (60.62%), Query Frame = 1

Query: 49  VTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGF 108
           + +++VFGDSSVD GNNN + T  +SNF PYG+DF   +PTGRFC+G++ATDF++EA+G 
Sbjct: 26  IPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRDFVGGKPTGRFCNGKIATDFMSEALGL 85

Query: 109 GETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRL 168
              +PA+LD +    D   GV+FASA++GYD+ T+++  VL L KQLEY+  Y+  L   
Sbjct: 86  KPIIPAYLDPSYNISDFATGVTFASAATGYDNATSDVLSVLPLWKQLEYYKEYQTKLKAY 145

Query: 169 VGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHR 228
            G D+    I +++ ++S+GTNDFLENYF  P R  Q+S+  YQ+FL       V+ +H 
Sbjct: 146 QGKDRGTETIESSLYLISIGTNDFLENYFAFPGRSSQYSVSLYQDFLAGIAKEFVKKLHG 205

Query: 229 LGIRRLVVVGVPPLGCMPVVRTITNQIT--TCSTSFNRAAYAFNAKIKLKLAAMEAHL-G 288
           LG R++ + G+PP+GCMP+ R  TN  T   C   +N  A  FN+K+   +  +   L G
Sbjct: 206 LGARKISLGGLPPMGCMPLERA-TNIGTGGECVGRYNDIAVQFNSKLDKMVEKLSKELPG 265

Query: 289 LLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGST--TCSDPQSYVFW 348
               F + Y      + NP+++G       CC TG+ E G  C+ +   TC++   YVFW
Sbjct: 266 SNLVFSNPYEPFMRIIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNPFTCTNADKYVFW 325

Query: 349 DAVHPSQNMYKILAAKAIQS 364
           D+ HP+Q    I+A   + S
Sbjct: 326 DSFHPTQKTNHIMANALMNS 344

BLAST of CmoCh07G002050 vs. TrEMBL
Match: A0A0A0KLL4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G518320 PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 1.3e-171
Identity = 305/373 (81.77%), Postives = 334/373 (89.54%), Query Frame = 1

Query: 4   MGTRVKNAVLLLFIMGIGFCPPLMEGKE------IAEVNVQQLRKLASKYNVTSLLVFGD 63
           M  R++   LL+ +MG+ FCP LMEGK+      I EVNV+QLRKLA KYNVTSLLVFGD
Sbjct: 1   MAMRLEKLSLLITLMGLSFCPSLMEGKKENSNDFIGEVNVKQLRKLAWKYNVTSLLVFGD 60

Query: 64  SSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGETVPAFLD 123
           SSVDPGNNN LST +KSNFPPYGKDFFNARPTGRFCDGRLATDFIAEA+GFGETVPAFLD
Sbjct: 61  SSVDPGNNNFLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLD 120

Query: 124 RTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVGYDKAENI 183
           RTLKPI+LLHGVSFASASSGYDDLTAN S VLSLPKQLEY MHYK+HL R VG +KAE I
Sbjct: 121 RTLKPIELLHGVSFASASSGYDDLTANYSNVLSLPKQLEYLMHYKLHLKRQVGGEKAEKI 180

Query: 184 IRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLGIRRLVVV 243
           I+NAIVV+SMGTNDFLENYFLEP R KQFSLDQYQNFLVSSM  NV+VMHRLG+RRLVVV
Sbjct: 181 IKNAIVVISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMYRNVQVMHRLGVRRLVVV 240

Query: 244 GVPPLGCMPVVRTITNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHLGLLTSFVDAYAIV 303
           GVPPLGCMPVVRTITNQ TTCS  FN+AAYAFNAK+KLKLA ++A LG+LTSFVDAYAIV
Sbjct: 241 GVPPLGCMPVVRTITNQNTTCSEVFNQAAYAFNAKMKLKLAGIKASLGMLTSFVDAYAIV 300

Query: 304 QDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGSTTCSDPQSYVFWDAVHPSQNMYKILA 363
           Q AV NPTAYGLRETAKGCCGTGLVE+GETCKGS TCSDP++Y+FWDAVHPS+ MYKILA
Sbjct: 301 QAAVHNPTAYGLRETAKGCCGTGLVEYGETCKGSPTCSDPENYLFWDAVHPSEKMYKILA 360

Query: 364 AKAIQSVAQDILS 371
           A+AI+SV Q+ILS
Sbjct: 361 AQAIRSVQQNILS 373

BLAST of CmoCh07G002050 vs. TrEMBL
Match: B9H2M9_POPTR (GDSL-motif lipase/hydrolase family protein OS=Populus trichocarpa GN=POPTR_0004s05120g PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.6e-124
Identity = 217/353 (61.47%), Postives = 281/353 (79.60%), Query Frame = 1

Query: 12  VLLLFIMGIGFCPPLMEGKEIAEVNVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTN 71
           V ++ ++ +    PL  G     V+++QLR+L +K+NVT +LVFGDSSVDPGNNN L T 
Sbjct: 5   VPMVTVLALMALMPLFSGA----VDIRQLRQLTAKHNVTCVLVFGDSSVDPGNNNRLPTT 64

Query: 72  VKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGETVPAFLDRTLKPIDLLHGVSF 131
           VK NFPPYGKDFF+ RPTGRF +GRLATDFIAEA+G+ + +PAFLD  LKP DLLHGVSF
Sbjct: 65  VKGNFPPYGKDFFDRRPTGRFSNGRLATDFIAEAIGYTKIIPAFLDPNLKPTDLLHGVSF 124

Query: 132 ASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVGYDKAENIIRNAIVVLSMGTND 191
           ASA+SGYDDLTANLS+VL + KQLEY  HYK+HLSRLVG  KA+NI+ NAI +LSMGTND
Sbjct: 125 ASAASGYDDLTANLSQVLPVSKQLEYLKHYKLHLSRLVGVKKAQNIVNNAIFLLSMGTND 184

Query: 192 FLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLGIRRLVVVGVPPLGCMPVVRTI 251
           FL+NY+LEP R KQF+++QYQNFL S M  +++ M+RLG  R+VVVGVPPLGCMP+VRT+
Sbjct: 185 FLQNYYLEPNRPKQFNVEQYQNFLASRMFEDIKEMNRLGATRVVVVGVPPLGCMPLVRTL 244

Query: 252 TNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHLGLLTSFVDAYAIVQDAVDNPTAYGLRE 311
             Q  TC  S+N+ A++ NAKIK KLA ++  +G+  ++VD Y ++Q+A++ P  +GL E
Sbjct: 245 AGQ-NTCVESYNQVAWSLNAKIKEKLAILKKTIGIKDAYVDCYGVIQNAINTPKKFGLVE 304

Query: 312 TAKGCCGTGLVEFGETCKGSTTCSDPQSYVFWDAVHPSQNMYKILAAKAIQSV 365
           T+KGCCG+G +E+G+TCKG TTC+DP  Y FWDAVHP++ MY+ILA +AI S+
Sbjct: 305 TSKGCCGSGTIEYGDTCKGMTTCADPSKYAFWDAVHPTEKMYRILADEAIASL 352

BLAST of CmoCh07G002050 vs. TrEMBL
Match: A0A059CCJ1_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D00574 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 7.9e-124
Identity = 211/336 (62.80%), Postives = 273/336 (81.25%), Query Frame = 1

Query: 35  VNVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCD 94
           + ++++R+LA+K NVT +LVFGDSSVDPGNNNVL T  K NFPPYG+DFF  RPTGRFC+
Sbjct: 27  IAIEKVRQLAAKNNVTCVLVFGDSSVDPGNNNVLQTTFKGNFPPYGRDFFKGRPTGRFCN 86

Query: 95  GRLATDFIAEAMGFGETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQ 154
           GRLATDFIAEA+G+ + +PAFLD  L   DLL+GVSFASA+SGYDDLTAN+S VLS+ +Q
Sbjct: 87  GRLATDFIAEALGYTKAIPAFLDPKLTKSDLLYGVSFASAASGYDDLTANMSSVLSVSQQ 146

Query: 155 LEYFMHYKIHLSRLVGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNF 214
           LEYF+HYKIHL +LVG  KAE II NA+ V+SMGTNDFL+N+FL+P R +Q+SL  Y NF
Sbjct: 147 LEYFLHYKIHLRKLVGVKKAEEIINNAVFVMSMGTNDFLQNFFLDPTRPRQYSLRAYINF 206

Query: 215 LVSSMSHNVEVMHRLGIRRLVVVGVPPLGCMPVVRTITNQITTCSTSFNRAAYAFNAKIK 274
           L+S MS +V+ MHR+G RR+VVVG+PPLGCMP+V+T+    TTC  +FN  +++FN+ I+
Sbjct: 207 LISCMSRDVQEMHRMGARRVVVVGIPPLGCMPLVKTL-KDTTTCYQAFNNLSFSFNSLIR 266

Query: 275 LKLAAMEAHLGLLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGSTTC 334
            KL ++   LGL T+FVD YAIV DA+  P  YGL ET+KGCCGTG +E+G+TCKG TTC
Sbjct: 267 RKLHSLGKSLGLKTAFVDVYAIVLDAIKRPHRYGLTETSKGCCGTGTIEYGDTCKGLTTC 326

Query: 335 SDPQSYVFWDAVHPSQNMYKILAAKAIQSVAQDILS 371
           S+P+ YVFWDAVHP++ MYKI+A +A+  + +++LS
Sbjct: 327 SEPEKYVFWDAVHPTEKMYKIVALEALALIGENVLS 361

BLAST of CmoCh07G002050 vs. TrEMBL
Match: F6HM59_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0003g00620 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 9.6e-122
Identity = 214/366 (58.47%), Postives = 284/366 (77.60%), Query Frame = 1

Query: 4   MGTRVKNAVLLLFIMGIGFCPPLMEGKEIAEVNVQQLRKLASKYNVTSLLVFGDSSVDPG 63
           M  R++ A+L+LF + +    PL+       V++ QLR+LA++ NVT +LVFGDSSVDPG
Sbjct: 59  MVMRMRLALLVLFSLAV---TPLLA----RAVDIHQLRQLAARNNVTCILVFGDSSVDPG 118

Query: 64  NNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGETVPAFLDRTLKPI 123
           NNN L T +K NFPPYGK+F N RPTGRF +GRLATDFIAEA+G+   +PAFLD  ++  
Sbjct: 119 NNNQLDTMMKGNFPPYGKNFLNGRPTGRFSNGRLATDFIAEALGYRNIIPAFLDPHIQKA 178

Query: 124 DLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVGYDKAENIIRNAIV 183
           DLLHGVSFAS++SGYDDLTANLS V  + KQLEYF+HYKIHL +LVG  KAE I+  A+ 
Sbjct: 179 DLLHGVSFASSASGYDDLTANLSNVFPVSKQLEYFLHYKIHLRQLVGKKKAEEILGRALF 238

Query: 184 VLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLGIRRLVVVGVPPLG 243
           V+SMGTNDFL+NYFLEP R +Q++L++Y+N+L+S M+H++E MHRLG RRLVVVG+PPLG
Sbjct: 239 VMSMGTNDFLQNYFLEPTRSEQYTLEEYENYLISCMAHDIEEMHRLGARRLVVVGIPPLG 298

Query: 244 CMPVVRTITNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHLGLLTSFVDAYAIVQDAVDN 303
           CMP+V+T+ ++ T+C  S+N+AA +FN+KIK KLA +   L L T++ D Y  V+ A++N
Sbjct: 299 CMPLVKTLKDE-TSCVESYNQAAASFNSKIKEKLAILRTSLRLKTAYADIYGTVERAMNN 358

Query: 304 PTAYGLRETAKGCCGTGLVEFGETCKGSTTCSDPQSYVFWDAVHPSQNMYKILAAKAIQS 363
           P  YG   T KGCCG+G VE+ E+C+G +TC+DP  Y+FWDAVHPS+NMYKI+A   + S
Sbjct: 359 PKQYGFTVTTKGCCGSGTVEYAESCRGLSTCADPSKYLFWDAVHPSENMYKIIADDVVNS 416

Query: 364 VAQDIL 370
           + QD+L
Sbjct: 419 LDQDLL 416

BLAST of CmoCh07G002050 vs. TrEMBL
Match: M5WTP3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007642mg PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.4e-120
Identity = 209/361 (57.89%), Postives = 286/361 (79.22%), Query Frame = 1

Query: 9   KNAVLLLFIMGIGFCPPLMEGKEIAEVNVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVL 68
           K   L++  + +    PL+ G     +++ + R++A++YNVT +LVFGDSSVD GNNN +
Sbjct: 4   KKGTLVIVELALLLLLPLLSGA----LDIVKAREIAARYNVTCILVFGDSSVDSGNNNFI 63

Query: 69  STNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGETVPAFLDRTLKPIDLLHG 128
            T +KSNF PYGKDFFN RPTGRF +GRLATDFIA+A+G+ +T+P FLD  LK  DL HG
Sbjct: 64  VTTIKSNFLPYGKDFFNGRPTGRFSNGRLATDFIADAIGYTKTIPPFLDPNLKLEDLPHG 123

Query: 129 VSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVGYDKAENIIRNAIVVLSMG 188
            SFASA+SGYDD TAN+S VLSLP+QLEY MHYK+ L +LVG  +AE+IIR A+ V+SMG
Sbjct: 124 ASFASAASGYDDFTANISSVLSLPRQLEYLMHYKVKLRKLVGQKRAEDIIRTALFVMSMG 183

Query: 189 TNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLGIRRLVVVGVPPLGCMPVV 248
           TNDFL++Y+L+P R KQF++++YQN+LVS M++ ++ M RLG  RLVVVGVPPLGCMP+V
Sbjct: 184 TNDFLQDYYLDPTRPKQFTVEEYQNYLVSCMANAIQKMQRLGGTRLVVVGVPPLGCMPLV 243

Query: 249 RTITNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHLGLLTSFVDAYAIVQDAVDNPTAYG 308
           +TI + +TTC+ ++N+ A++ N+KI+ KL  + A   + T+FVDAY I+Q+A++NP  YG
Sbjct: 244 KTIMD-VTTCAENYNKVAFSLNSKIQEKLETISATSRMETAFVDAYGIIQNAINNPALYG 303

Query: 309 LRETAKGCCGTGLVEFGETCKGSTTCSDPQSYVFWDAVHPSQNMYKILAAKAIQSVAQDI 368
           L ET+KGCCGTG +EFG++C+G  TC+DP+ YVFWDAVHP++ MYKILA +AI+++AQ +
Sbjct: 304 LSETSKGCCGTGTIEFGDSCRGLETCTDPEKYVFWDAVHPTEKMYKILAEEAIKALAQKL 359

Query: 369 L 370
           L
Sbjct: 364 L 359

BLAST of CmoCh07G002050 vs. TAIR10
Match: AT5G45950.1 (AT5G45950.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 389.8 bits (1000), Expect = 1.9e-108
Identity = 184/338 (54.44%), Postives = 250/338 (73.96%), Query Frame = 1

Query: 32  IAEVNVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGR 91
           +  ++V  LR+LA+K+NVTS+LVFGDSSVDPGNNN + T +K NFPPYG++F N +PTGR
Sbjct: 21  VLSLDVHLLRQLAAKHNVTSILVFGDSSVDPGNNNFIKTEMKGNFPPYGENFINHKPTGR 80

Query: 92  FCDGRLATDFIAEAMGFGETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSL 151
            CDG LA D+IAEAMG+   +PAFLD +L   DL  G SFASA SGYDDLTAN+S V S 
Sbjct: 81  LCDGLLAPDYIAEAMGY-PPIPAFLDPSLTQADLTRGASFASAGSGYDDLTANISNVWSF 140

Query: 152 PKQLEYFMHYKIHLSRLVGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQY 211
             Q  YF+HYKIHL++LVG  ++  +I NAI ++SMG+NDFL+NY ++  R+KQF+++QY
Sbjct: 141 TTQANYFLHYKIHLTKLVGPLESAKMINNAIFLMSMGSNDFLQNYLVDFTRQKQFTVEQY 200

Query: 212 QNFLVSSMSHNVEVMHRLGIRRLVVVGVPPLGCMPVVRTITNQITTCSTSFNRAAYAFNA 271
             FL   M ++ +++HRLG +RLVVVGVPP+GCMP+++ +  Q  TC    N+ A++FNA
Sbjct: 201 IEFLSHRMLYDAKMLHRLGAKRLVVVGVPPMGCMPLIKYLRGQ-KTCVDQLNQIAFSFNA 260

Query: 272 KIKLKLAAMEAHLGLLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGS 331
           KI   L  +++ +GL T +VDAY+ +Q+A+ NP  +G  E + GCCGTG  E+GETCK  
Sbjct: 261 KIIKNLELLQSKIGLKTIYVDAYSTIQEAIKNPRKFGFVEASLGCCGTGTYEYGETCKDM 320

Query: 332 TTCSDPQSYVFWDAVHPSQNMYKILAAKAIQSVAQDIL 370
             C DP  YVFWDAVHP+Q MY+I+  KAI S++++ L
Sbjct: 321 QVCKDPTKYVFWDAVHPTQRMYQIIVKKAIASISEEFL 356

BLAST of CmoCh07G002050 vs. TAIR10
Match: AT2G42990.1 (AT2G42990.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 275.4 bits (703), Expect = 5.1e-74
Identity = 138/313 (44.09%), Postives = 202/313 (64.54%), Query Frame = 1

Query: 49  VTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGF 108
           + +++VFGDSSVD GNNN +ST  ++NF PYG+DF   R TGRFC+GRL++DF +EA G 
Sbjct: 26  IPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGGRATGRFCNGRLSSDFTSEAYGL 85

Query: 109 GETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRL 168
             TVPA+LD +    D   GV FASA +GYD+ TA++  V+ L K++EYF  Y+ +LS  
Sbjct: 86  KPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVLGVIPLWKEVEYFKEYQSNLSAY 145

Query: 169 VGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHR 228
           +G+ +A  IIR ++ ++S+GTNDFLENY+  P RR QFS+ QYQ+FLV      ++ ++R
Sbjct: 146 LGHRRAAKIIRESLYIVSIGTNDFLENYYTLPDRRSQFSISQYQDFLVEIAEVFLKDIYR 205

Query: 229 LGIRRLVVVGVPPLGCMPVVR-TITNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHL-GL 288
           LG R++   G+ P+GC+P+ R T  +   +C+ S+N  A  FN +++  +  +   L G+
Sbjct: 206 LGARKMSFTGISPMGCLPLERVTNLDDPFSCARSYNDLAVDFNGRLRRLVTKLNRELTGI 265

Query: 289 LTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETC--KGSTTCSDPQSYVFWD 348
              F + Y I+ D V  P  YGL  ++  CCGTGL E G  C      TCSD   +VFWD
Sbjct: 266 KIYFANPYDIMWDIVTKPNLYGLEISSSACCGTGLFEMGFLCGQDNPLTCSDANKFVFWD 325

Query: 349 AVHPSQNMYKILA 358
           A HP++   +I++
Sbjct: 326 AFHPTERTNQIVS 338

BLAST of CmoCh07G002050 vs. TAIR10
Match: AT5G45960.1 (AT5G45960.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 274.2 bits (700), Expect = 1.1e-73
Identity = 140/318 (44.03%), Postives = 200/318 (62.89%), Query Frame = 1

Query: 46  KYNVTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEA 105
           K++V+++LVFGDS+VDPGNNN + T  K NFPPYG DF N  PTGRFC+GRL TDFIA  
Sbjct: 42  KHSVSAILVFGDSTVDPGNNNYIDTVFKCNFPPYGLDFRNKTPTGRFCNGRLVTDFIASY 101

Query: 106 MGFGETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHL 165
           +G  E VP +LD  L   +L+ GVSFASA SGYD LT  ++ V+ +P QLEYF  YK  L
Sbjct: 102 IGVKENVPPYLDPNLGINELISGVSFASAGSGYDPLTPTITNVIDIPTQLEYFREYKRKL 161

Query: 166 SRLVGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEV 225
              +G  + E  I  A+  +S GTNDF+ NYF  P RRK F+++ YQ F++S++   ++ 
Sbjct: 162 EGKMGKQEMEKHIEEAMFCVSAGTNDFVINYFTIPIRRKTFTIEAYQQFVISNLKQFIQG 221

Query: 226 MHRLGIRRLVVVGVPPLGCMPVVRT------ITNQITTCSTSFNRAAYAFNAKIKLKLAA 285
           + + G R++ V G+PP+GC+P+V T      +TN+   C   F+  A  +N  ++ +LA 
Sbjct: 222 LWKEGARKITVAGLPPIGCLPIVITLFSGEALTNR--RCIDRFSTVATNYNFLLQKQLAL 281

Query: 286 ME---AHLGLLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCK-GSTTCS 345
           M+   AHLG    ++D Y  V + + +P  +G  E   GCCG+G +E    C   S  C 
Sbjct: 282 MQVGLAHLGSKIFYLDVYNPVYEVIRDPRKFGFEEVFSGCCGSGYLEASFLCNPKSYVCP 341

Query: 346 DPQSYVFWDAVHPSQNMY 354
           +  +YVF+D++HPS+  Y
Sbjct: 342 NTSAYVFFDSIHPSEKTY 357

BLAST of CmoCh07G002050 vs. TAIR10
Match: AT1G06990.1 (AT1G06990.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 268.1 bits (684), Expect = 8.2e-72
Identity = 132/319 (41.38%), Postives = 201/319 (63.01%), Query Frame = 1

Query: 51  SLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGE 110
           ++LVFGDS++D GNNN + T +++NFPPYG +F     TGRF +G+L  DFIA  MG  +
Sbjct: 37  AILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGIKD 96

Query: 111 TVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVG 170
           TVP FLD  L   D++ GV FASA SGYD+LT   +  LS+ KQ +    Y   LS++VG
Sbjct: 97  TVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQIVG 156

Query: 171 YDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLG 230
            +KA +I+  A+V++S GTNDF  N +  P RR++  +D YQ+F++S++ + V+ ++ +G
Sbjct: 157 DEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYDIG 216

Query: 231 IRRLVVVGVPPLGCMPVVRTITNQITT---CSTSFNRAAYAFNAKIKLKLAAMEAHL-GL 290
            R+++V+G+PP+GC+P+  T+  Q      C    N  +  FN K+K  L  M+++L G 
Sbjct: 217 CRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLTGS 276

Query: 291 LTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGST-TCSDPQSYVFWDA 350
           +  + D Y  + D   NP  YGL+ET +GCCGTG +E    C   T  C +P  Y+FWD 
Sbjct: 277 VIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYLFWDD 336

Query: 351 VHPSQNMYKILAAKAIQSV 365
           +HPSQ  Y +++   ++ +
Sbjct: 337 IHPSQIAYIVISLSLVEQI 355

BLAST of CmoCh07G002050 vs. TAIR10
Match: AT2G04570.1 (AT2G04570.1 GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 256.5 bits (654), Expect = 2.5e-68
Identity = 131/320 (40.94%), Postives = 194/320 (60.62%), Query Frame = 1

Query: 49  VTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGF 108
           + +++VFGDSSVD GNNN + T  +SNF PYG+DF   +PTGRFC+G++ATDF++EA+G 
Sbjct: 26  IPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRDFVGGKPTGRFCNGKIATDFMSEALGL 85

Query: 109 GETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRL 168
              +PA+LD +    D   GV+FASA++GYD+ T+++  VL L KQLEY+  Y+  L   
Sbjct: 86  KPIIPAYLDPSYNISDFATGVTFASAATGYDNATSDVLSVLPLWKQLEYYKEYQTKLKAY 145

Query: 169 VGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHR 228
            G D+    I +++ ++S+GTNDFLENYF  P R  Q+S+  YQ+FL       V+ +H 
Sbjct: 146 QGKDRGTETIESSLYLISIGTNDFLENYFAFPGRSSQYSVSLYQDFLAGIAKEFVKKLHG 205

Query: 229 LGIRRLVVVGVPPLGCMPVVRTITNQIT--TCSTSFNRAAYAFNAKIKLKLAAMEAHL-G 288
           LG R++ + G+PP+GCMP+ R  TN  T   C   +N  A  FN+K+   +  +   L G
Sbjct: 206 LGARKISLGGLPPMGCMPLERA-TNIGTGGECVGRYNDIAVQFNSKLDKMVEKLSKELPG 265

Query: 289 LLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGST--TCSDPQSYVFW 348
               F + Y      + NP+++G       CC TG+ E G  C+ +   TC++   YVFW
Sbjct: 266 SNLVFSNPYEPFMRIIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNPFTCTNADKYVFW 325

Query: 349 DAVHPSQNMYKILAAKAIQS 364
           D+ HP+Q    I+A   + S
Sbjct: 326 DSFHPTQKTNHIMANALMNS 344

BLAST of CmoCh07G002050 vs. NCBI nr
Match: gi|449434308|ref|XP_004134938.1| (PREDICTED: GDSL esterase/lipase At5g45950 [Cucumis sativus])

HSP 1 Score: 610.5 bits (1573), Expect = 1.9e-171
Identity = 305/373 (81.77%), Postives = 334/373 (89.54%), Query Frame = 1

Query: 4   MGTRVKNAVLLLFIMGIGFCPPLMEGKE------IAEVNVQQLRKLASKYNVTSLLVFGD 63
           M  R++   LL+ +MG+ FCP LMEGK+      I EVNV+QLRKLA KYNVTSLLVFGD
Sbjct: 1   MAMRLEKLSLLITLMGLSFCPSLMEGKKENSNDFIGEVNVKQLRKLAWKYNVTSLLVFGD 60

Query: 64  SSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGETVPAFLD 123
           SSVDPGNNN LST +KSNFPPYGKDFFNARPTGRFCDGRLATDFIAEA+GFGETVPAFLD
Sbjct: 61  SSVDPGNNNFLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLD 120

Query: 124 RTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVGYDKAENI 183
           RTLKPI+LLHGVSFASASSGYDDLTAN S VLSLPKQLEY MHYK+HL R VG +KAE I
Sbjct: 121 RTLKPIELLHGVSFASASSGYDDLTANYSNVLSLPKQLEYLMHYKLHLKRQVGGEKAEKI 180

Query: 184 IRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLGIRRLVVV 243
           I+NAIVV+SMGTNDFLENYFLEP R KQFSLDQYQNFLVSSM  NV+VMHRLG+RRLVVV
Sbjct: 181 IKNAIVVISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMYRNVQVMHRLGVRRLVVV 240

Query: 244 GVPPLGCMPVVRTITNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHLGLLTSFVDAYAIV 303
           GVPPLGCMPVVRTITNQ TTCS  FN+AAYAFNAK+KLKLA ++A LG+LTSFVDAYAIV
Sbjct: 241 GVPPLGCMPVVRTITNQNTTCSEVFNQAAYAFNAKMKLKLAGIKASLGMLTSFVDAYAIV 300

Query: 304 QDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGSTTCSDPQSYVFWDAVHPSQNMYKILA 363
           Q AV NPTAYGLRETAKGCCGTGLVE+GETCKGS TCSDP++Y+FWDAVHPS+ MYKILA
Sbjct: 301 QAAVHNPTAYGLRETAKGCCGTGLVEYGETCKGSPTCSDPENYLFWDAVHPSEKMYKILA 360

Query: 364 AKAIQSVAQDILS 371
           A+AI+SV Q+ILS
Sbjct: 361 AQAIRSVQQNILS 373

BLAST of CmoCh07G002050 vs. NCBI nr
Match: gi|659078553|ref|XP_008439783.1| (PREDICTED: GDSL esterase/lipase At5g45950 [Cucumis melo])

HSP 1 Score: 610.1 bits (1572), Expect = 2.5e-171
Identity = 303/370 (81.89%), Postives = 333/370 (90.00%), Query Frame = 1

Query: 7   RVKNAVLLLFIMGIGFCPPLMEGKE------IAEVNVQQLRKLASKYNVTSLLVFGDSSV 66
           +++  +LL+ +MG+GFCP LMEGK+      I +VNV+QLRK+A KYNVTSLLVFGDSSV
Sbjct: 5   QLEKLLLLITLMGLGFCPSLMEGKKENSNDFIGDVNVKQLRKMAWKYNVTSLLVFGDSSV 64

Query: 67  DPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGETVPAFLDRTL 126
           DPGNNN L T +KSNFPPYGKDFFNARPTGRFCDGRLATDFIAEA+GFGETVPAFLDRTL
Sbjct: 65  DPGNNNFLPTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDRTL 124

Query: 127 KPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVGYDKAENIIRN 186
           KPI+LLHGVSFASASSGYDDLTAN S VLSLPKQLEY MHYK+HL R VG +KAE IIRN
Sbjct: 125 KPIELLHGVSFASASSGYDDLTANYSNVLSLPKQLEYLMHYKLHLKRQVGGEKAEKIIRN 184

Query: 187 AIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLGIRRLVVVGVP 246
           AIVV+SMGTNDFLENYFLEP R KQF+LDQYQNFLVSSM  NV+VMHRLG+RRLVVVGVP
Sbjct: 185 AIVVISMGTNDFLENYFLEPLRPKQFTLDQYQNFLVSSMYRNVQVMHRLGVRRLVVVGVP 244

Query: 247 PLGCMPVVRTITNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHLGLLTSFVDAYAIVQDA 306
           PLGCMPVVRTITNQ TTCS  FN+AAYAFNAK+KLKLA ++A LG+LTSFVDAYAIVQ A
Sbjct: 245 PLGCMPVVRTITNQNTTCSKVFNQAAYAFNAKMKLKLAGIKASLGMLTSFVDAYAIVQAA 304

Query: 307 VDNPTAYGLRETAKGCCGTGLVEFGETCKGSTTCSDPQSYVFWDAVHPSQNMYKILAAKA 366
           V NPTAYGLRETA+GCCGTGLVE+GETCKGS TC+DPQ+YVFWDAVHPSQ MYKILAA+A
Sbjct: 305 VQNPTAYGLRETARGCCGTGLVEYGETCKGSPTCTDPQNYVFWDAVHPSQKMYKILAAQA 364

Query: 367 IQSVAQDILS 371
           IQSV  DILS
Sbjct: 365 IQSVQHDILS 374

BLAST of CmoCh07G002050 vs. NCBI nr
Match: gi|224077024|ref|XP_002305097.1| (GDSL-motif lipase/hydrolase family protein [Populus trichocarpa])

HSP 1 Score: 454.1 bits (1167), Expect = 2.3e-124
Identity = 217/353 (61.47%), Postives = 281/353 (79.60%), Query Frame = 1

Query: 12  VLLLFIMGIGFCPPLMEGKEIAEVNVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTN 71
           V ++ ++ +    PL  G     V+++QLR+L +K+NVT +LVFGDSSVDPGNNN L T 
Sbjct: 5   VPMVTVLALMALMPLFSGA----VDIRQLRQLTAKHNVTCVLVFGDSSVDPGNNNRLPTT 64

Query: 72  VKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGETVPAFLDRTLKPIDLLHGVSF 131
           VK NFPPYGKDFF+ RPTGRF +GRLATDFIAEA+G+ + +PAFLD  LKP DLLHGVSF
Sbjct: 65  VKGNFPPYGKDFFDRRPTGRFSNGRLATDFIAEAIGYTKIIPAFLDPNLKPTDLLHGVSF 124

Query: 132 ASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVGYDKAENIIRNAIVVLSMGTND 191
           ASA+SGYDDLTANLS+VL + KQLEY  HYK+HLSRLVG  KA+NI+ NAI +LSMGTND
Sbjct: 125 ASAASGYDDLTANLSQVLPVSKQLEYLKHYKLHLSRLVGVKKAQNIVNNAIFLLSMGTND 184

Query: 192 FLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLGIRRLVVVGVPPLGCMPVVRTI 251
           FL+NY+LEP R KQF+++QYQNFL S M  +++ M+RLG  R+VVVGVPPLGCMP+VRT+
Sbjct: 185 FLQNYYLEPNRPKQFNVEQYQNFLASRMFEDIKEMNRLGATRVVVVGVPPLGCMPLVRTL 244

Query: 252 TNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHLGLLTSFVDAYAIVQDAVDNPTAYGLRE 311
             Q  TC  S+N+ A++ NAKIK KLA ++  +G+  ++VD Y ++Q+A++ P  +GL E
Sbjct: 245 AGQ-NTCVESYNQVAWSLNAKIKEKLAILKKTIGIKDAYVDCYGVIQNAINTPKKFGLVE 304

Query: 312 TAKGCCGTGLVEFGETCKGSTTCSDPQSYVFWDAVHPSQNMYKILAAKAIQSV 365
           T+KGCCG+G +E+G+TCKG TTC+DP  Y FWDAVHP++ MY+ILA +AI S+
Sbjct: 305 TSKGCCGSGTIEYGDTCKGMTTCADPSKYAFWDAVHPTEKMYRILADEAIASL 352

BLAST of CmoCh07G002050 vs. NCBI nr
Match: gi|702318342|ref|XP_010052242.1| (PREDICTED: GDSL esterase/lipase At5g45950 [Eucalyptus grandis])

HSP 1 Score: 451.8 bits (1161), Expect = 1.1e-123
Identity = 211/336 (62.80%), Postives = 273/336 (81.25%), Query Frame = 1

Query: 35  VNVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTNVKSNFPPYGKDFFNARPTGRFCD 94
           + ++++R+LA+K NVT +LVFGDSSVDPGNNNVL T  K NFPPYG+DFF  RPTGRFC+
Sbjct: 27  IAIEKVRQLAAKNNVTCVLVFGDSSVDPGNNNVLQTTFKGNFPPYGRDFFKGRPTGRFCN 86

Query: 95  GRLATDFIAEAMGFGETVPAFLDRTLKPIDLLHGVSFASASSGYDDLTANLSKVLSLPKQ 154
           GRLATDFIAEA+G+ + +PAFLD  L   DLL+GVSFASA+SGYDDLTAN+S VLS+ +Q
Sbjct: 87  GRLATDFIAEALGYTKAIPAFLDPKLTKSDLLYGVSFASAASGYDDLTANMSSVLSVSQQ 146

Query: 155 LEYFMHYKIHLSRLVGYDKAENIIRNAIVVLSMGTNDFLENYFLEPQRRKQFSLDQYQNF 214
           LEYF+HYKIHL +LVG  KAE II NA+ V+SMGTNDFL+N+FL+P R +Q+SL  Y NF
Sbjct: 147 LEYFLHYKIHLRKLVGVKKAEEIINNAVFVMSMGTNDFLQNFFLDPTRPRQYSLRAYINF 206

Query: 215 LVSSMSHNVEVMHRLGIRRLVVVGVPPLGCMPVVRTITNQITTCSTSFNRAAYAFNAKIK 274
           L+S MS +V+ MHR+G RR+VVVG+PPLGCMP+V+T+    TTC  +FN  +++FN+ I+
Sbjct: 207 LISCMSRDVQEMHRMGARRVVVVGIPPLGCMPLVKTL-KDTTTCYQAFNNLSFSFNSLIR 266

Query: 275 LKLAAMEAHLGLLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEFGETCKGSTTC 334
            KL ++   LGL T+FVD YAIV DA+  P  YGL ET+KGCCGTG +E+G+TCKG TTC
Sbjct: 267 RKLHSLGKSLGLKTAFVDVYAIVLDAIKRPHRYGLTETSKGCCGTGTIEYGDTCKGLTTC 326

Query: 335 SDPQSYVFWDAVHPSQNMYKILAAKAIQSVAQDILS 371
           S+P+ YVFWDAVHP++ MYKI+A +A+  + +++LS
Sbjct: 327 SEPEKYVFWDAVHPTEKMYKIVALEALALIGENVLS 361

BLAST of CmoCh07G002050 vs. NCBI nr
Match: gi|743846396|ref|XP_011027739.1| (PREDICTED: GDSL esterase/lipase At5g45950 [Populus euphratica])

HSP 1 Score: 450.7 bits (1158), Expect = 2.5e-123
Identity = 219/358 (61.17%), Postives = 282/358 (78.77%), Query Frame = 1

Query: 12  VLLLFIMGIGFCPPLMEGKEIAEVNVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTN 71
           V ++ ++ +    PL  G     V+++QLR L +K+NVT +LVFGDSSVDPGNNN L T 
Sbjct: 5   VPMVTVLALMALMPLFSGA----VDIRQLRHLTAKHNVTCVLVFGDSSVDPGNNNRLPTT 64

Query: 72  VKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGETVPAFLDRTLKPIDLLHGVSF 131
            K NFPPYGKDFF+ RPTGR  +GRLATDFIAEA+G+ + +PAFLD  LKP DLLHGVSF
Sbjct: 65  AKGNFPPYGKDFFDRRPTGRLSNGRLATDFIAEAIGYTKIIPAFLDPNLKPADLLHGVSF 124

Query: 132 ASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVGYDKAENIIRNAIVVLSMGTND 191
           ASA+SGYDDLTANLS+VL + KQLEY  HYK+HLSRLVG  KA+NII NAI VLSMGTND
Sbjct: 125 ASAASGYDDLTANLSQVLPVSKQLEYLKHYKLHLSRLVGVKKAQNIINNAIFVLSMGTND 184

Query: 192 FLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLGIRRLVVVGVPPLGCMPVVRTI 251
           FL+NY+LEP R KQF+++QYQNFL S M  +++ M+RLG  R+VVVGVPPLGCMP+VRT+
Sbjct: 185 FLQNYYLEPNRPKQFNVEQYQNFLASRMFEDIKGMNRLGATRVVVVGVPPLGCMPIVRTL 244

Query: 252 TNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHLGLLTSFVDAYAIVQDAVDNPTAYGLRE 311
             Q  TC  S+N+ A++FNAKIK KLA ++  +G+  ++VD Y ++Q+A++ P  +GL E
Sbjct: 245 AGQ-NTCVESYNQVAWSFNAKIKEKLAILK-KIGIKDAYVDCYGVIQNAINTPKKFGLVE 304

Query: 312 TAKGCCGTGLVEFGETCKGSTTCSDPQSYVFWDAVHPSQNMYKILAAKAIQSVAQDIL 370
           T+KGCCG+G VE+G+TCKG TTC+DP  Y FWDAVHP++ MY+ILA +AI S+ + ++
Sbjct: 305 TSKGCCGSGTVEYGDTCKGLTTCADPSKYAFWDAVHPTEKMYQILADEAIASLGRALI 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GDL85_ARATH3.3e-10754.44GDSL esterase/lipase At5g45950 OS=Arabidopsis thaliana GN=At5g45950 PE=2 SV=1[more]
GDL48_ARATH9.1e-7344.09GDSL esterase/lipase At2g42990 OS=Arabidopsis thaliana GN=At2g42990 PE=2 SV=1[more]
GDL86_ARATH2.0e-7244.03GDSL esterase/lipase At5g45960 OS=Arabidopsis thaliana GN=At5g45960 PE=2 SV=1[more]
GDL1_ARATH1.5e-7041.38GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana GN=At1g06990 PE=2 SV=2[more]
GDL34_ARATH4.4e-6740.94GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana GN=At2g04570 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KLL4_CUCSA1.3e-17181.77Uncharacterized protein OS=Cucumis sativus GN=Csa_6G518320 PE=4 SV=1[more]
B9H2M9_POPTR1.6e-12461.47GDSL-motif lipase/hydrolase family protein OS=Populus trichocarpa GN=POPTR_0004s... [more]
A0A059CCJ1_EUCGR7.9e-12462.80Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D00574 PE=4 SV=1[more]
F6HM59_VITVI9.6e-12258.47Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0003g00620 PE=4 SV=... [more]
M5WTP3_PRUPE1.4e-12057.89Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007642mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G45950.11.9e-10854.44 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT2G42990.15.1e-7444.09 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT5G45960.11.1e-7344.03 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT1G06990.18.2e-7241.38 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT2G04570.12.5e-6840.94 GDSL-like Lipase/Acylhydrolase superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449434308|ref|XP_004134938.1|1.9e-17181.77PREDICTED: GDSL esterase/lipase At5g45950 [Cucumis sativus][more]
gi|659078553|ref|XP_008439783.1|2.5e-17181.89PREDICTED: GDSL esterase/lipase At5g45950 [Cucumis melo][more]
gi|224077024|ref|XP_002305097.1|2.3e-12461.47GDSL-motif lipase/hydrolase family protein [Populus trichocarpa][more]
gi|702318342|ref|XP_010052242.1|1.1e-12362.80PREDICTED: GDSL esterase/lipase At5g45950 [Eucalyptus grandis][more]
gi|743846396|ref|XP_011027739.1|2.5e-12361.17PREDICTED: GDSL esterase/lipase At5g45950 [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001087GDSL
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052889 9,9'-di-cis-zeta-carotene desaturation to 7,9,7',9'-tetra-cis-lycopene
biological_process GO:0042335 cuticle development
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0000038 very long-chain fatty acid metabolic process
biological_process GO:0044710 single-organism metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0052887 7,9,9'-tricis-neurosporene:quinone oxidoreductase activity
molecular_function GO:0052886 9,9'-dicis-carotene:quinone oxidoreductase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0016635 oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh07G002050.1CmoCh07G002050.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 53..358
score: 3.3
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 1..363
score: 3.5E
NoneNo IPR availablePANTHERPTHR22835:SF218SUBFAMILY NOT NAMEDcoord: 1..363
score: 3.5E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh07G002050CmoCh19G010800Cucurbita moschata (Rifu)cmocmoB399
CmoCh07G002050CmoCh03G013040Cucurbita moschata (Rifu)cmocmoB459