CmoCh06G015250 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh06G015250
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Pectinesterase (3.1.1.11)
Location	Cmo_Chr06 : 10830265 .. 10830906 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTATGACGGTGGTGGATGCAAGAACAATAAGGCTGCCCTGGTTGGGTTGTTGTCTTTGTTTTTTGTTGCCTTGGTTAACGGTGTAAGTGGCGCAGGAGAGGAACCTGGCTCCTCTAATGCTGCCATTAAGACCCTATGTGAGCCCACTACTTACCGTGAAACTTGCGAAGAAGTCCTGGTGAAGTCCAATGTTGACTCAAAAGACCCACGTGAGTTGGTCAAGGCAGGGTTCCATTTTGCTATTGAGCAGCTCAAAGCGGCCATTGCAAATTCCCCCACTTTGAAACAAGCCGCCACTGACCCCATGGCGCAAAAAGCAGTTGATGCTTGCGACGAGCTCATGGATTATGCAATTGATGATCTTTTAACTTCCTTTGATAAAATCACCGATAGTTTTGATGTACACAGAGTGAATGCTTATCTTGAGAACCTTAGGATTTGGTTGAGCGCTGCATTGACATATCAAGAAACTTGTTTGGATGGTTTCGAGAATGTTCCTGGCGATACTGGTGAAAAGATGAAGAATTTGTTGAAGACGTCAAAAGAAATGACCGCCAATGGACTATCAAGTTTATGCAAAACTGTACCTCACACTATTGAGGACATATTCTATTTATATTGGCAAAAGATTAACTAA

mRNA sequence

ATGGCGTATGACGGTGGTGGATGCAAGAACAATAAGGCTGCCCTGGTTGGGTTGTTGTCTTTGTTTTTTGTTGCCTTGGTTAACGGTGTAAGTGGCGCAGGAGAGGAACCTGGCTCCTCTAATGCTGCCATTAAGACCCTATGTGAGCCCACTACTTACCGTGAAACTTGCGAAGAAGTCCTGGTGAAGTCCAATGTTGACTCAAAAGACCCACGTGAGTTGGTCAAGGCAGGGTTCCATTTTGCTATTGAGCAGCTCAAAGCGGCCATTGCAAATTCCCCCACTTTGAAACAAGCCGCCACTGACCCCATGGCGCAAAAAGCAGTTGATGCTTGCGACGAGCTCATGGATTATGCAATTGATGATCTTTTAACTTCCTTTGATAAAATCACCGATAGTTTTGATGTACACAGAGTGAATGCTTATCTTGAGAACCTTAGGATTTGGTTGAGCGCTGCATTGACATATCAAGAAACTTGTTTGGATGGTTTCGAGAATGTTCCTGGCGATACTGGTGAAAAGATGAAGAATTTGTTGAAGACGTCAAAAGAAATGACCGCCAATGGACTATCAAGTTTATGCAAAACTGTACCTCACACTATTGAGGACATATTCTATTTATATTGGCAAAAGATTAACTAA

Coding sequence (CDS)

ATGGCGTATGACGGTGGTGGATGCAAGAACAATAAGGCTGCCCTGGTTGGGTTGTTGTCTTTGTTTTTTGTTGCCTTGGTTAACGGTGTAAGTGGCGCAGGAGAGGAACCTGGCTCCTCTAATGCTGCCATTAAGACCCTATGTGAGCCCACTACTTACCGTGAAACTTGCGAAGAAGTCCTGGTGAAGTCCAATGTTGACTCAAAAGACCCACGTGAGTTGGTCAAGGCAGGGTTCCATTTTGCTATTGAGCAGCTCAAAGCGGCCATTGCAAATTCCCCCACTTTGAAACAAGCCGCCACTGACCCCATGGCGCAAAAAGCAGTTGATGCTTGCGACGAGCTCATGGATTATGCAATTGATGATCTTTTAACTTCCTTTGATAAAATCACCGATAGTTTTGATGTACACAGAGTGAATGCTTATCTTGAGAACCTTAGGATTTGGTTGAGCGCTGCATTGACATATCAAGAAACTTGTTTGGATGGTTTCGAGAATGTTCCTGGCGATACTGGTGAAAAGATGAAGAATTTGTTGAAGACGTCAAAAGAAATGACCGCCAATGGACTATCAAGTTTATGCAAAACTGTACCTCACACTATTGAGGACATATTCTATTTATATTGGCAAAAGATTAACTAA

Homology

[Close]

BLAST of CmoCh06G015250 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 147.9 bits (372), Expect = 1.3e-34
Identity = 76/201 (37.81%), Postives = 121/201 (60.20%), Query Frame = 1


Query: 3   YDGGGCKNNKAALVGLLSLFFVALV------------NGVSGAGEEPGSSNAAIKTLCEP 62
           YD    +  +  ++ + S+  +++V            +G S    E  +S  A+K +C P
Sbjct: 5   YDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAP 64

Query: 63  TTYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVD 122
           T YR+TCE+ L+K+  ++ DP ELVK  F+  ++Q+  A   S T+ +   D   + A+D
Sbjct: 65  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 124

Query: 123 ACDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGD 182
            C ELMDYA+D+L  SF+++   F+ H ++  L NLRIWLSAA++++ETCL+GF+   G+
Sbjct: 125 QCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGN 184

Query: 183 TGEKMKNLLKTSKEMTANGLS 192
            GE MK  LKT+ E+T NGL+
Sbjct: 185 AGETMKKALKTAIELTHNGLA 204

[Close]

BLAST of CmoCh06G015250 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 6.2e-34
Identity = 84/200 (42.00%), Postives = 119/200 (59.50%), Query Frame = 1


Query: 3   YDGGGCKNNKAALV-----GLLSLFFVALVNGVS-----GAG-EEPGSSNAAIKTLCEPT 62
           YD    K  K  ++      LL    VA+  GVS      AG EE  +S  AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 63  TYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDA 122
            Y+ETCE+ L K   D+ DP ELVK  F+  ++Q+      S T+ +   DP A+ A+D 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 123 CDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDT 182
           C ELMDYAI +L  SF+++   F+ H+V+  L  LRIWLSA +++++TCLDGF+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 183 GEKMKNLLKTSKEMTANGLS 192
           GE +K  LKT+ ++T NGL+
Sbjct: 185 GETIKKALKTAVQLTHNGLA 203

[Close]

BLAST of CmoCh06G015250 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.7e-26
Identity = 64/202 (31.68%), Postives = 110/202 (54.46%), Query Frame = 1


Query: 6   GGCKNNKAALVGLLSLFFVALVNGVSGAGEEPGS------------SNAAIKTLCEPTTY 65
           G  K  K  + G+++   V +V  V        S            +  A++ +C PT Y
Sbjct: 5   GELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDY 64

Query: 66  RETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAQKAVDA 125
           +ETC   L+K++ DS  P +L+K GF+  I  ++ +I  A+     +AA D   + A++ 
Sbjct: 65  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 124

Query: 126 CDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDT 185
           C++LM+ A DDL    D   D F + ++  ++E+LR+WLS ++ YQ+TC+D FE      
Sbjct: 125 CEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKL 184

Query: 186 GEKMKNLLKTSKEMTANGLSSL 194
            + M+ + KTS+E+T+NGL+ +
Sbjct: 185 SQDMQKIFKTSRELTSNGLAMI 205

[Close]

BLAST of CmoCh06G015250 vs. Swiss-Prot
Match: PME23_ARATH (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=PME23 PE=2 SV=3)

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-22
Identity = 59/200 (29.50%), Postives = 109/200 (54.50%), Query Frame = 1


Query: 7   GCKNNKAALVGLLSLFFVALVNGVSGAGEEPG---------SSNAAIKTLCEPTTYRETC 66
           G K  K  + G +S F V +V  V+    +            +  A++ +C PT +++TC
Sbjct: 5   GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTC 64

Query: 67  EEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAQKAVDACDEL 126
              L+ ++ DS DP +L+K GF   I+ +  ++  A+     +A  +P A+ A + C++L
Sbjct: 65  VNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKL 124

Query: 127 MDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 186
           M  AIDDL    D     F V ++  ++E+LR+WLS ++ +Q+TC+D F  +  +  + M
Sbjct: 125 MIDAIDDLKKCMDH---GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 184

Query: 187 KNLLKTSKEMTANGLSSLCK 196
             + KTS+E+++N L+ + +
Sbjct: 185 LKIFKTSRELSSNSLAMVTR 201

[Close]

BLAST of CmoCh06G015250 vs. Swiss-Prot
Match: PME43_ARATH (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana GN=PME43 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.8e-20
Identity = 58/196 (29.59%), Postives = 102/196 (52.04%), Query Frame = 1


Query: 11  NKAALVGLLSLFFVALVNGVSGAGEEPGS-------------SNAAIKTLCEPTTYRETC 70
           NK  L+G+ +L    ++   +  G  P               +   +  +C  T Y++ C
Sbjct: 2   NKYVLLGVTALIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQDC 61

Query: 71  EEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANS--PTLKQAATDPMAQKAVDACDEL 130
              L  + V S DPR L+++ F  AI  +++ I         +A  D   ++A++ C EL
Sbjct: 62  TTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCREL 121

Query: 131 MDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 190
           MD AIDDL  + DK    F   R++ ++E+L +WLS ++TYQ+TC+DGFE +  +    M
Sbjct: 122 MDDAIDDLRKTRDKFR-GFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMM 181

Query: 191 KNLLKTSKEMTANGLS 192
           + +++  + +T+NGL+
Sbjct: 182 ERVMRKGQHLTSNGLA 194

[Close]

BLAST of CmoCh06G015250 vs. TrEMBL
Match: A0A0A0L930_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G164510 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 8.2e-57
Identity = 113/198 (57.07%), Postives = 150/198 (75.76%), Query Frame = 1


Query: 9   KNNKAALVGLLSLFFVALVNGV-----------SGAGE-----EPGSSNAAIKTLCEPTT 68
           KN KAAL+G+ SLF VA+V  V           SG G+     E  +S  A+K LC+PT 
Sbjct: 6   KNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTD 65

Query: 69  YRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDAC 128
           Y+ETCE+ L ++  ++ DPREL+KAGF+ A+ ++K AI NS TLK+AA+DPMA++A+DAC
Sbjct: 66  YQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQALDAC 125

Query: 129 DELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTG 188
            ELMDYAIDDL+ SF +ITD+FD+ +++ Y+E+L++WLS ALTYQETC+DGFENV GDTG
Sbjct: 126 GELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTGDTG 185

Query: 189 EKMKNLLKTSKEMTANGL 191
           EKM  LL+TSKE+T NGL
Sbjct: 186 EKMTKLLETSKELTINGL 203

[Close]

BLAST of CmoCh06G015250 vs. TrEMBL
Match: M5XK28_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa025631mg PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-45
Identity = 98/199 (49.25%), Postives = 139/199 (69.85%), Query Frame = 1


Query: 5   GGGCKNNKA-ALVGLLSLFFVALVNGVS-----------GAGEEPGSSNAAIKTLCEPTT 64
           GG  +N K  A++G+ +L  VA+V  V+             GE+  +S  AI+++C+PT 
Sbjct: 3   GGEVQNKKKMAIIGVSALILVAMVVAVTVGVTVSRHKGKSGGEQTSTSTKAIQSICQPTD 62

Query: 65  YRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDAC 124
           Y++TCE+ L K   +  DP+ELVKAGF  AI+QL+  I NS TLK+ A DP A +A+  C
Sbjct: 63  YKKTCEDNLSKVASNVTDPKELVKAGFQVAIDQLREVIKNSTTLKELAKDPRANQALQNC 122

Query: 125 DELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTG 184
            EL++YAIDDL  SFDK+   FD  +++AY+E+L++WLSAA+TY++TCLDGFEN  GD G
Sbjct: 123 KELLEYAIDDLGDSFDKL-GPFDFTKLDAYVEDLKVWLSAAMTYEQTCLDGFENTTGDAG 182

Query: 185 EKMKNLLKTSKEMTANGLS 192
           EKM+  LKTS+E+T+NGL+
Sbjct: 183 EKMRQFLKTSQELTSNGLA 200

[Close]

BLAST of CmoCh06G015250 vs. TrEMBL
Match: A0A061EQE7_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_021251 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.4e-43
Identity = 95/196 (48.47%), Postives = 136/196 (69.39%), Query Frame = 1


Query: 12  KAALVGLLSLFFVALVNGVS------------GAGEEP----GSSNAAIKTLCEPTTYRE 71
           K A++ + S+F VA+V  V+            G G  P     +S  AIK +C+PT YRE
Sbjct: 125 KVAIISVCSVFLVAMVVAVAVGVSRSHGGRGNGGGATPTGEISTSTKAIKAICQPTDYRE 184

Query: 72  TCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDEL 131
           TCE+ L  S+ ++ DP+EL+K GF  AI ++K  IANS T+K+ A DPMA++A++ C EL
Sbjct: 185 TCEKSL--SSANTTDPKELIKIGFQAAISEIKKVIANSSTVKEVAKDPMARQALENCHEL 244

Query: 132 MDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 191
           MDYAIDDL  SF+++ + FD  +++ Y+ENL+IWL  A+TYQ+TCLDGF N  G+ G+KM
Sbjct: 245 MDYAIDDLKNSFNQLGE-FDFSKLDEYIENLKIWLGGAITYQQTCLDGFMNTTGEAGKKM 304

[Close]

BLAST of CmoCh06G015250 vs. TrEMBL
Match: M5XQF1_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa026560mg PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.4e-43
Identity = 94/200 (47.00%), Postives = 138/200 (69.00%), Query Frame = 1


Query: 5   GGGCKNNKAALVGLLSLFFVALVN----GVSGAGEEPGS---------SNAAIKTLCEPT 64
           G   K  K A++ + SLF VA+V     GV+ +  + GS         S  AI+ +C+PT
Sbjct: 3   GDNNKKKKIAIIAVSSLFLVAMVIAVTVGVTTSRSKGGSKSNDGQTTTSTKAIQAICQPT 62

Query: 65  TYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDA 124
            Y+ETC++ L  + V+S +P+EL+KAGF  A+++L   I NS TL++ A DP A +A++ 
Sbjct: 63  DYKETCQKSLESAGVNSTEPKELIKAGFKVAMDKLHEVIKNSTTLQELAKDPGANQALEN 122

Query: 125 CDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDT 184
           C EL+DYAI DL  SFDK+   FD+ +++AY E+L+IWLSAA+TY++TCLDGF+N  GD 
Sbjct: 123 CKELLDYAIGDLAASFDKL-GPFDISKIDAYAEDLKIWLSAAMTYEQTCLDGFQNTTGDA 182

Query: 185 GEKMKNLLKTSKEMTANGLS 192
           GEKMK LL +++E+T+NGL+
Sbjct: 183 GEKMKRLLNSTQELTSNGLA 201

[Close]

BLAST of CmoCh06G015250 vs. TrEMBL
Match: M5XBV5_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa003441mg PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 8.8e-43
Identity = 94/199 (47.24%), Postives = 136/199 (68.34%), Query Frame = 1


Query: 5   GGGCKNNKA-ALVGLLSLFFVALVNGVS-----------GAGEEPGSSNAAIKTLCEPTT 64
           GG  +N K  A++G+ +L  VA+V  V+             GE+  +S  AI+++C+PT 
Sbjct: 3   GGEVQNKKKIAIIGVSALILVAMVVAVTVGVTVSRHKGKSGGEQTSTSTKAIQSICQPTD 62

Query: 65  YRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDAC 124
           Y++TCE+ L K   +  DP+ELVKAGF  AI QL+  I NS TLK+ A D  A +A+  C
Sbjct: 63  YKKTCEDNLSKVASNVTDPKELVKAGFQVAIGQLREVIKNSTTLKELAKDSRANQALQNC 122

Query: 125 DELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTG 184
            EL++YAIDDL  SFDK+   FD  ++++Y+E+L++WLSAA+TY++TCLDGF+N  GD G
Sbjct: 123 KELLEYAIDDLGDSFDKL-GPFDFTKLDSYVEDLKVWLSAAMTYEQTCLDGFQNTTGDAG 182

Query: 185 EKMKNLLKTSKEMTANGLS 192
            KM+  LKTS+E+T+NGL+
Sbjct: 183 AKMRQFLKTSQELTSNGLA 200

[Close]

BLAST of CmoCh06G015250 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 147.9 bits (372), Expect = 7.1e-36
Identity = 76/201 (37.81%), Postives = 121/201 (60.20%), Query Frame = 1


Query: 3   YDGGGCKNNKAALVGLLSLFFVALV------------NGVSGAGEEPGSSNAAIKTLCEP 62
           YD    +  +  ++ + S+  +++V            +G S    E  +S  A+K +C P
Sbjct: 5   YDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAP 64

Query: 63  TTYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVD 122
           T YR+TCE+ L+K+  ++ DP ELVK  F+  ++Q+  A   S T+ +   D   + A+D
Sbjct: 65  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 124

Query: 123 ACDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGD 182
            C ELMDYA+D+L  SF+++   F+ H ++  L NLRIWLSAA++++ETCL+GF+   G+
Sbjct: 125 QCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGN 184

Query: 183 TGEKMKNLLKTSKEMTANGLS 192
            GE MK  LKT+ E+T NGL+
Sbjct: 185 AGETMKKALKTAIELTHNGLA 204

[Close]

BLAST of CmoCh06G015250 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 145.6 bits (366), Expect = 3.5e-35
Identity = 84/200 (42.00%), Postives = 119/200 (59.50%), Query Frame = 1


Query: 3   YDGGGCKNNKAALV-----GLLSLFFVALVNGVS-----GAG-EEPGSSNAAIKTLCEPT 62
           YD    K  K  ++      LL    VA+  GVS      AG EE  +S  AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 63  TYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDA 122
            Y+ETCE+ L K   D+ DP ELVK  F+  ++Q+      S T+ +   DP A+ A+D 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 123 CDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDT 182
           C ELMDYAI +L  SF+++   F+ H+V+  L  LRIWLSA +++++TCLDGF+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 183 GEKMKNLLKTSKEMTANGLS 192
           GE +K  LKT+ ++T NGL+
Sbjct: 185 GETIKKALKTAVQLTHNGLA 203

[Close]

BLAST of CmoCh06G015250 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 119.8 bits (299), Expect = 2.1e-27
Identity = 64/202 (31.68%), Postives = 110/202 (54.46%), Query Frame = 1


Query: 6   GGCKNNKAALVGLLSLFFVALVNGVSGAGEEPGS------------SNAAIKTLCEPTTY 65
           G  K  K  + G+++   V +V  V        S            +  A++ +C PT Y
Sbjct: 5   GELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDY 64

Query: 66  RETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAQKAVDA 125
           +ETC   L+K++ DS  P +L+K GF+  I  ++ +I  A+     +AA D   + A++ 
Sbjct: 65  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 124

Query: 126 CDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDT 185
           C++LM+ A DDL    D   D F + ++  ++E+LR+WLS ++ YQ+TC+D FE      
Sbjct: 125 CEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKL 184

Query: 186 GEKMKNLLKTSKEMTANGLSSL 194
            + M+ + KTS+E+T+NGL+ +
Sbjct: 185 SQDMQKIFKTSRELTSNGLAMI 205

[Close]

BLAST of CmoCh06G015250 vs. TAIR10
Match: AT3G06830.1 (AT3G06830.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 107.5 bits (267), Expect = 1.1e-23
Identity = 59/200 (29.50%), Postives = 109/200 (54.50%), Query Frame = 1


Query: 7   GCKNNKAALVGLLSLFFVALVNGVSGAGEEPG---------SSNAAIKTLCEPTTYRETC 66
           G K  K  + G +S F V +V  V+    +            +  A++ +C PT +++TC
Sbjct: 5   GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTC 64

Query: 67  EEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAQKAVDACDEL 126
              L+ ++ DS DP +L+K GF   I+ +  ++  A+     +A  +P A+ A + C++L
Sbjct: 65  VNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKL 124

Query: 127 MDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 186
           M  AIDDL    D     F V ++  ++E+LR+WLS ++ +Q+TC+D F  +  +  + M
Sbjct: 125 MIDAIDDLKKCMDH---GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 184

Query: 187 KNLLKTSKEMTANGLSSLCK 196
             + KTS+E+++N L+ + +
Sbjct: 185 LKIFKTSRELSSNSLAMVTR 201

[Close]

BLAST of CmoCh06G015250 vs. TAIR10
Match: AT4G15980.1 (AT4G15980.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 100.9 bits (250), Expect = 9.9e-22
Identity = 58/196 (29.59%), Postives = 102/196 (52.04%), Query Frame = 1


Query: 11  NKAALVGLLSLFFVALVNGVSGAGEEPGS-------------SNAAIKTLCEPTTYRETC 70
           NK  L+G+ +L    ++   +  G  P               +   +  +C  T Y++ C
Sbjct: 2   NKYVLLGVTALIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQDC 61

Query: 71  EEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANS--PTLKQAATDPMAQKAVDACDEL 130
              L  + V S DPR L+++ F  AI  +++ I         +A  D   ++A++ C EL
Sbjct: 62  TTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCREL 121

Query: 131 MDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKM 190
           MD AIDDL  + DK    F   R++ ++E+L +WLS ++TYQ+TC+DGFE +  +    M
Sbjct: 122 MDDAIDDLRKTRDKFR-GFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMM 181

Query: 191 KNLLKTSKEMTANGLS 192
           + +++  + +T+NGL+
Sbjct: 182 ERVMRKGQHLTSNGLA 194

[Close]

BLAST of CmoCh06G015250 vs. NCBI nr
Match: gi|659076869|ref|XP_008438906.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 237.3 bits (604), Expect = 2.5e-59
Identity = 118/203 (58.13%), Postives = 153/203 (75.37%), Query Frame = 1


Query: 4   DGGGCKNNKAALVGLLSLFFVALV----------------NGVSGAGEEPGSSNAAIKTL 63
           DGG  KN KAAL+G+ SLF VA+V                +G  GA  E  +S  A++ L
Sbjct: 3   DGG--KNKKAALIGVSSLFLVAMVVAVAVGVNRKNPTTDSSGGDGATAEISTSTKAVQAL 62

Query: 64  CEPTTYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQK 123
           C+PT Y+ETCE+ L ++  ++ DPREL+K+GFH A+ ++K AI NS  LK+AA+DPMA++
Sbjct: 63  CQPTDYKETCEKALSEAGTNTSDPRELIKSGFHVAVNEIKWAIGNSTQLKEAASDPMAKQ 122

Query: 124 AVDACDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENV 183
           A+DAC ELMDYAIDDL+ SFD+ITD+FDV ++N Y+E+L+IWLS ALTYQETC+DGFENV
Sbjct: 123 ALDACGELMDYAIDDLVISFDRITDNFDVQKLNDYVEDLKIWLSGALTYQETCIDGFENV 182

Query: 184 PGDTGEKMKNLLKTSKEMTANGL 191
            GDTGEKM  LLKTSKE+T+NGL
Sbjct: 183 TGDTGEKMAKLLKTSKELTSNGL 203

[Close]

BLAST of CmoCh06G015250 vs. NCBI nr
Match: gi|778678954|ref|XP_004134493.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-56
Identity = 113/198 (57.07%), Postives = 150/198 (75.76%), Query Frame = 1


Query: 9   KNNKAALVGLLSLFFVALVNGV-----------SGAGE-----EPGSSNAAIKTLCEPTT 68
           KN KAAL+G+ SLF VA+V  V           SG G+     E  +S  A+K LC+PT 
Sbjct: 6   KNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTD 65

Query: 69  YRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDAC 128
           Y+ETCE+ L ++  ++ DPREL+KAGF+ A+ ++K AI NS TLK+AA+DPMA++A+DAC
Sbjct: 66  YQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQALDAC 125

Query: 129 DELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTG 188
            ELMDYAIDDL+ SF +ITD+FD+ +++ Y+E+L++WLS ALTYQETC+DGFENV GDTG
Sbjct: 126 GELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTGDTG 185

Query: 189 EKMKNLLKTSKEMTANGL 191
           EKM  LL+TSKE+T NGL
Sbjct: 186 EKMTKLLETSKELTINGL 203

[Close]

BLAST of CmoCh06G015250 vs. NCBI nr
Match: gi|596252541|ref|XP_007224682.1| (hypothetical protein PRUPE_ppa025631mg [Prunus persica])

HSP 1 Score: 191.0 bits (484), Expect = 2.1e-45
Identity = 98/199 (49.25%), Postives = 139/199 (69.85%), Query Frame = 1


Query: 5   GGGCKNNKA-ALVGLLSLFFVALVNGVS-----------GAGEEPGSSNAAIKTLCEPTT 64
           GG  +N K  A++G+ +L  VA+V  V+             GE+  +S  AI+++C+PT 
Sbjct: 3   GGEVQNKKKMAIIGVSALILVAMVVAVTVGVTVSRHKGKSGGEQTSTSTKAIQSICQPTD 62

Query: 65  YRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDAC 124
           Y++TCE+ L K   +  DP+ELVKAGF  AI+QL+  I NS TLK+ A DP A +A+  C
Sbjct: 63  YKKTCEDNLSKVASNVTDPKELVKAGFQVAIDQLREVIKNSTTLKELAKDPRANQALQNC 122

Query: 125 DELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTG 184
            EL++YAIDDL  SFDK+   FD  +++AY+E+L++WLSAA+TY++TCLDGFEN  GD G
Sbjct: 123 KELLEYAIDDLGDSFDKL-GPFDFTKLDAYVEDLKVWLSAAMTYEQTCLDGFENTTGDAG 182

Query: 185 EKMKNLLKTSKEMTANGLS 192
           EKM+  LKTS+E+T+NGL+
Sbjct: 183 EKMRQFLKTSQELTSNGLA 200

[Close]

BLAST of CmoCh06G015250 vs. NCBI nr
Match: gi|645281117|ref|XP_008245480.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Prunus mume])

HSP 1 Score: 190.7 bits (483), Expect = 2.7e-45
Identity = 95/194 (48.97%), Postives = 136/194 (70.10%), Query Frame = 1


Query: 9   KNNKAALVGLLSLFFVALVNGVS-----------GAGEEPGSSNAAIKTLCEPTTYRETC 68
           K  K A++G+ +L  VA+V  V+             GE+  +S  AI+++C+PT Y++TC
Sbjct: 9   KRKKIAIIGVSALILVAMVVAVTVGVTVSRHKGKSGGEQTSTSTKAIQSICQPTDYKKTC 68

Query: 69  EEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVDACDELMD 128
           E+ L K   +  DP+ELVKAGF  AI+QL+  I NS TLK+ A DP + +A+  C EL++
Sbjct: 69  EDNLSKVASNVTDPKELVKAGFQVAIDQLREVIKNSTTLKELAKDPSSNQALQNCKELLE 128

Query: 129 YAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGDTGEKMKN 188
           YAIDDL  SFDK+   FD  +++AY+E+L++WLSAA+TY++TCLDGFEN  GD GEKM+ 
Sbjct: 129 YAIDDLGDSFDKL-GPFDFTKLDAYVEDLKVWLSAAMTYEQTCLDGFENTTGDAGEKMRQ 188

Query: 189 LLKTSKEMTANGLS 192
            LKTS+E+T+NGL+
Sbjct: 189 FLKTSQELTSNGLA 201

[Close]

BLAST of CmoCh06G015250 vs. NCBI nr
Match: gi|1009163283|ref|XP_015899883.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 189.9 bits (481), Expect = 4.6e-45
Identity = 95/201 (47.26%), Postives = 138/201 (68.66%), Query Frame = 1


Query: 4   DGGGCKNNKAALVGLLSLFFVALVNGVSGAGEEPGS-------------SNAAIKTLCEP 63
           DGG  K  K A++G  +L  VA+V  V+    + GS             S+ A+K +C P
Sbjct: 8   DGGNGKKKKVAIMGASALILVAMVVAVAVGVHKGGSDDDDHSSSGSISTSSKAVKQICAP 67

Query: 64  TTYRETCEEVLVKSNVDSKDPRELVKAGFHFAIEQLKAAIANSPTLKQAATDPMAQKAVD 123
           T YRETCE  L  S+V+S DP+EL+KAGF  A++ L+ AI NS TLK+ A DP A +A+ 
Sbjct: 68  TDYRETCETSL--SSVNSTDPKELIKAGFQVAVQHLRVAIENSTTLKELAKDPRANQALS 127

Query: 124 ACDELMDYAIDDLLTSFDKITDSFDVHRVNAYLENLRIWLSAALTYQETCLDGFENVPGD 183
            C+ELMDYAIDD+  SF+++  +FD+ ++  Y+E+L++WL  A+TY++TCLDGFEN  GD
Sbjct: 128 NCEELMDYAIDDIQDSFNRL-GAFDISKLEEYVEDLKVWLGGAVTYEQTCLDGFENTTGD 187

Query: 184 TGEKMKNLLKTSKEMTANGLS 192
            G++MK +LKT++E+T+NGL+
Sbjct: 188 AGDRMKAILKTAQELTSNGLA 205

The following BLAST results are available for this feature:

BLAST of CmoCh06G015250 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PME21_ARATH	1.3e-34	37.81	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=P...	[more]
PME28_ARATH	6.2e-34	42.00	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=P...	[more]
PME58_ARATH	3.7e-26	31.68	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=P...	[more]
PME23_ARATH	1.9e-22	29.50	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=P...	[more]
PME43_ARATH	1.8e-20	29.59	Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana GN=P...	[more]

BLAST of CmoCh06G015250 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L930_CUCSA	8.2e-57	57.07	Pectinesterase OS=Cucumis sativus GN=Csa_3G164510 PE=4 SV=1	[more]
M5XK28_PRUPE	1.4e-45	49.25	Pectinesterase OS=Prunus persica GN=PRUPE_ppa025631mg PE=4 SV=1	[more]
A0A061EQE7_THECC	1.4e-43	48.47	Pectinesterase OS=Theobroma cacao GN=TCM_021251 PE=4 SV=1	[more]
M5XQF1_PRUPE	1.4e-43	47.00	Pectinesterase OS=Prunus persica GN=PRUPE_ppa026560mg PE=4 SV=1	[more]
M5XBV5_PRUPE	8.8e-43	47.24	Pectinesterase OS=Prunus persica GN=PRUPE_ppa003441mg PE=4 SV=1	[more]

BLAST of CmoCh06G015250 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G05610.1	7.1e-36	37.81	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G27870.1	3.5e-35	42.00	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	2.1e-27	31.68	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	1.1e-23	29.50	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	9.9e-22	29.59	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

BLAST of CmoCh06G015250 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659076869\|ref\|XP_008438906.1\|	2.5e-59	58.13	PREDICTED: pectinesterase-like [Cucumis melo]	[more]
gi\|778678954\|ref\|XP_004134493.2\|	1.2e-56	57.07	PREDICTED: pectinesterase-like [Cucumis sativus]	[more]
gi\|596252541\|ref\|XP_007224682.1\|	2.1e-45	49.25	hypothetical protein PRUPE_ppa025631mg [Prunus persica]	[more]
gi\|645281117\|ref\|XP_008245480.1\|	2.7e-45	48.97	PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Prunus mume]	[more]
gi\|1009163283\|ref\|XP_015899883.1\|	4.6e-45	47.26	PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 isoform X1 [Zizip...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G015250.1	CmoCh06G015250.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 41..191 score: 4.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 43..190 score: 8.7
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 39..192 score: 3.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 20..190 score: 8.0
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 43..190 score: 7.85
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 11..191 score: 3.2
None	No IPR available	PANTHER	PTHR31707:SF17	SUBFAMILY NOT NAMED	coord: 11..191 score: 3.2

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh06G015250	Cucurbita maxima (Rimu)	cmacmoB271
CmoCh06G015250	Cucurbita maxima (Rimu)	cmacmoB829
CmoCh06G015250	Cucurbita maxima (Rimu)	cmacmoB835
CmoCh06G015250	Wild cucumber (PI 183967)	cmocpiB814
CmoCh06G015250	Cucumber (Chinese Long) v2	cmocuB806
CmoCh06G015250	Melon (DHL92) v3.5.1	cmomeB755
CmoCh06G015250	Watermelon (Charleston Gray)	cmowcgB743
CmoCh06G015250	Watermelon (97103) v1	cmowmB793
CmoCh06G015250	Cucurbita pepo (Zucchini)	cmocpeB787
CmoCh06G015250	Cucurbita pepo (Zucchini)	cmocpeB796
CmoCh06G015250	Bottle gourd (USVL1VR-Ls)	cmolsiB752
CmoCh06G015250	Cucumber (Gy14) v2	cgybcmoB401
CmoCh06G015250	Melon (DHL92) v3.6.1	cmomedB857
CmoCh06G015250	Silver-seed gourd	carcmoB0390
CmoCh06G015250	Cucumber (Chinese Long) v3	cmocucB0953
CmoCh06G015250	Watermelon (97103) v2	cmowmbB833
CmoCh06G015250	Wax gourd	cmowgoB1005
CmoCh06G015250	Cucurbita moschata (Rifu)	cmocmoB224
CmoCh06G015250	Cucumber (Gy14) v1	cgycmoB0718