CmoCh06G015150 (gene) Cucurbita moschata (Rifu)

BLAST of CmoCh06G015150 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 5.1e-160
Identity = 282/571 (49.39%), Postives = 383/571 (67.08%), Query Frame = 1

Query: 3   YDGGGCKINKVAALVGLLSLFVVAMVNGVN-----------GAEEHSESSSAIKTLCEPT 62
           YD    K  K   ++ + S+ +++MV  V            G EE + S  AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 63  DYRQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKALDD 122
           DY++TCE+ L K   D+ DP EL+K  F+  ++QI      S+T+ E   DP AK ALD 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 123 CSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDT 182
           C ELMDYAI +L  SF+++ + F+F K+D+ +  LRIWLS  ++++ TCLDGF+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEELGK-FEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 183 GEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLG-RELRTKESEEEEEVPSW 242
           GE +K  LK + ++T N L MV E+ + +  +     +P +  R L +      +E PSW
Sbjct: 185 GETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQ----IPEMNSRRLLS------QEFPSW 244

Query: 243 VNDR-RGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQ 302
           ++ R R LL A  + VK D VVA+DGSG+YKTI EA+  VPKK N TFV++IKEG+Y+E 
Sbjct: 245 MDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEY 304

Query: 303 VMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAGA 362
           V V++  T+++ IGDGP KT I+  +++ DG +T+KTATV+++G +FI K++ FEN+AGA
Sbjct: 305 VQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGA 364

Query: 363 AKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQN 422
            KHQAVA+RV +D SIFYNC+ DGYQDTLY H+HRQFYRDCTI+GTIDF+FG+AA V QN
Sbjct: 365 IKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQN 424

Query: 423 CKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKE 482
           C + VRKPL NQ C +TA GR   +E T  +LQ C I  +PDY  V+  +K YLGRPWKE
Sbjct: 425 CTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKE 484

Query: 483 YSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITA 542
           YSRTIIM + I D + PEGW PW+G F LNTLFYSE  N GPGA+   RV W GIK+++ 
Sbjct: 485 YSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSD 544

Query: 543 KHALDFTPALFIRGNPWIKPTGVPYSSGMMN 561
           +  L FTPA +I+G+ WI   GVPY  G+ +
Sbjct: 545 EEILKFTPAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of CmoCh06G015150 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 564.3 bits (1453), Expect = 1.5e-159
Identity = 278/553 (50.27%), Postives = 382/553 (69.08%), Query Frame = 1

Query: 19  LLSLFVVAMVNGVN---------GAEEHSESSSAIKTLCEPTDYRQTCEEALVKSKTDSQ 78
           LL   VVA+  GV+         G  E + S  A+K +C PTDYR+TCE+ L+K+  ++ 
Sbjct: 24  LLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNGKNTT 83

Query: 79  DPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKALDDCSELMDYAIDDLLLSFDK 138
           DP EL+K  F+  ++QI  A   S+T+ E   D   + ALD C ELMDYA+D+L  SF++
Sbjct: 84  DPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEE 143

Query: 139 ITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGEKMKHLLKLSQEMTANA 198
           + + F+F  +D+ + +LRIWLS A++++ TCL+GF+   G+ GE MK  LK + E+T N 
Sbjct: 144 LGK-FEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNG 203

Query: 199 LEMVGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEEVPSWVNDR-RGLLQATGA--NVK 258
           L ++ E+ + V  +     +P L       E       PSWV+ R R LLQA  A  +VK
Sbjct: 204 LAIISEMSNFVGQMQ----IPGLNSRRLLAEG-----FPSWVDQRGRKLLQAAAAYSDVK 263

Query: 259 ADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQVMVDKKTTYVMMIGDGP 318
            D VVA+DGSG+YKTI EA++ VPKK N TFV++IK G+Y+E V V+K  ++++ IGDGP
Sbjct: 264 PDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGP 323

Query: 319 TKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDMSIF 378
            KT I+  +N+ DG +T++TATV+++G  FI K++ FEN+AGA KHQAVA+RVQSD SIF
Sbjct: 324 DKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIF 383

Query: 379 YNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQNCKIFVRKPLENQKCIVT 438
           +NCR DGYQDTLYTH+HRQF+RDCTI+GTIDF+FG+AA V QNC + VRKPL NQ C +T
Sbjct: 384 FNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPIT 443

Query: 439 AQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKEYSRTIIMESQIDDLIQP 498
           A GR   +E T  + Q C I+ +PDY  V+  +KAYLGRPWKEYSRTIIM + I D +QP
Sbjct: 444 AHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQP 503

Query: 499 EGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHALDFTPALFIRGNPW 558
           +GW PW+G+F L TLFYSE  N GPG++  +RV W GIK ++ +  L FTPA +I+G+ W
Sbjct: 504 QGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDW 563

Query: 559 IKPTGVPYSSGMM 560
           I   GVPY++G++
Sbjct: 564 IPGKGVPYTTGLL 566

BLAST of CmoCh06G015150 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.4e-156
Identity = 274/551 (49.73%), Postives = 374/551 (67.88%), Query Frame = 1

Query: 16  LVGLLSLFVVAMVNGVNGAEEHSE---------SSSAIKTLCEPTDYRQTCEEALVKSKT 75
           +  LL L VVA+    +    HSE         +++A++ +C PTDY++TC  +L+K+  
Sbjct: 18  ITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSLMKASP 77

Query: 76  DSQDPRELIKAGFHYVVEQIKSAITNSKT--LKETASDPMAKKALDDCSELMDYAIDDLL 135
           DS  P +LIK GF+  +  I+ +I  +      + A+D   K AL+ C +LM+ A DDL 
Sbjct: 78  DSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDATDDLK 137

Query: 136 LSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGEKMKHLLKLSQE 195
              D   + F   +++D++EDLR+WLSG++ YQ TC+D FE       + M+ + K S+E
Sbjct: 138 KCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTSRE 197

Query: 196 MTANALEMVGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEEVPSWVNDRRGLLQATGAN 255
           +T+N L M+  + +++           LG+  R K    E+ +PSWV      L AT   
Sbjct: 198 LTSNGLAMITNISNLLGEFNVTGVTGDLGKYAR-KLLSAEDGIPSWVGPNTRRLMATKGG 257

Query: 256 VKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQVMVDKKTTYVMMIGD 315
           VKA+ VVA DGSG+YKTI EA+  VPK + K FVIYIK+GVY E+V V KK T+V  IGD
Sbjct: 258 VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGD 317

Query: 316 GPTKTKITARENFADG-TSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDM 375
           GPTKTKIT   N+  G   T+ TATV++ G NF  K++ FEN+AG   HQAVALRV +D+
Sbjct: 318 GPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADL 377

Query: 376 SIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQNCKIFVRKPLENQKC 435
           ++FYNC++DGYQDTLY H+HRQF+RDCT++GT+DFIFG+  VVLQNC I VRKP+++Q C
Sbjct: 378 AVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSC 437

Query: 436 IVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKEYSRTIIMESQIDDL 495
           ++TAQGR+ K+E T ++LQNC I+ +P Y PV+ INKAYLGRPWKE+SRTIIM + IDD+
Sbjct: 438 MITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTIIMGTTIDDV 497

Query: 496 IQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHALDFTPALFIRG 555
           I P GWLPW G+FALNTL+Y+E+ N GPG++   RVKW GIK+++ K AL FTPA F+RG
Sbjct: 498 IDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRG 557

BLAST of CmoCh06G015150 vs. Swiss-Prot
Match: PME23_ARATH (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=PME23 PE=2 SV=3)

HSP 1 Score: 490.0 bits (1260), Expect = 3.6e-137
Identity = 248/549 (45.17%), Postives = 366/549 (66.67%), Query Frame = 1

Query: 16  LVGLLSLFVVAMVNGVNGAEEH-SESSSAIKTLCEPTDYRQTCEEALVKSKTDSQDPREL 75
           ++ ++S+ VV   +     E H  +++ A++ +C PTD++ TC  +L+ +  DS DP +L
Sbjct: 22  VIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTCVNSLMGASPDSDDPVDL 81

Query: 76  IKAGFHYVVEQIKSAITN-SKTLKETAS-DPMAKKALDDCSELMDYAIDDLLLSFDKITE 135
           IK GF   ++ I  ++   S  +K  A  +P AK A + C +LM  AIDDL    D    
Sbjct: 82  IKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKLMIDAIDDLKKCMD---H 141

Query: 136 TFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGEKMKHLLKLSQEMTANALEM 195
            F   +++ ++EDLR+WLSG++ +Q TC+D F  +  +  + M  + K S+E+++N+L M
Sbjct: 142 GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDMLKIFKTSRELSSNSLAM 201

Query: 196 VGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEEVPSWVN-DRRGLLQATGAN---VKAD 255
           V  + +++ +     GL     +   K    E+ +P+WV  + R L+ A G     VKA+
Sbjct: 202 VTRISTLIPNS-NLTGLTGALAKYARKLLSTEDSIPTWVGPEARRLMAAQGGGPGPVKAN 261

Query: 256 AVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQVMVDKKTTYVMMIGDGPTK 315
           AVVA+DG+G++KTIT+A+  VPK +   F+I+IKEG+Y+E+V V KK  +V  IGDGP K
Sbjct: 262 AVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNK 321

Query: 316 TKITARENFADG-TSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDMSIFY 375
           T IT   NF  G   TF TAT+++ G +F  K++  EN+AG    QAVALRV +D ++F+
Sbjct: 322 TLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFH 381

Query: 376 NCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQNCKIFVRKPLENQKCIVTA 435
           +C++DG+QDTLY H+HRQFYRDCT++GT+DFIFG+A  +LQNCKI VRKP + Q C+VTA
Sbjct: 382 SCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTA 441

Query: 436 QGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKEYSRTIIMESQIDDLIQPE 495
           QGR+  +E T ++L  C I+ DP Y P++ +NKAYLGRPWKE+SRTIIM++ IDD+I P 
Sbjct: 442 QGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPA 501

Query: 496 GWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHALDFTPALFIRGNPWI 555
           GWLPW G+FAL TL+Y+E  N GPG++   RVKW GIK++T + AL +T   F+RG+ WI
Sbjct: 502 GWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWI 561

Query: 556 KPTGVPYSS 557
             T VPY++
Sbjct: 562 PQTQVPYTA 566

BLAST of CmoCh06G015150 vs. Swiss-Prot
Match: PME_MEDSA (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 2.0e-119
Identity = 214/457 (46.83%), Postives = 303/457 (66.30%), Query Frame = 1

Query: 109 LDDCSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVT 168
           ++ C+E++DYA+D +  S   + + FDF K+ +Y  DL++WL+G L++Q TCLDGF N T
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQ-FDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTT 60

Query: 169 GDTGEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEEVP 228
              GE M  +LK S E+++NA++M+  V  ++           + R L +     ++ +P
Sbjct: 61  TKAGETMTKVLKTSMELSSNAIDMMDAVSRILKGF--DTSQYSVSRRLLS-----DDGIP 120

Query: 229 SWVNDRRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEE 288
           SWVND    L A G NV+ +AVVA+DGSG++KT+T+A++ VP K+   FVI++K GVY+E
Sbjct: 121 SWVNDGHRRLLA-GGNVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKE 180

Query: 289 QVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAG 348
            V V K+  YV +IGDGPTKTK T   N+ADG +T+ TAT  V G NF+ KD+ FEN+AG
Sbjct: 181 TVNVAKEMNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAG 240

Query: 349 AAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQ 408
             KHQAVALRV +D +IFYNC+MDG+QDTLY  + RQFYRDC+I+GTIDF+FG    V Q
Sbjct: 241 TGKHQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQ 300

Query: 409 NCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWK 468
           NCK+  R P + Q+C+VTA GR ++   +A++ Q+   + +P    V     +YLGRPWK
Sbjct: 301 NCKLVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPALTSVTP-KVSYLGRPWK 360

Query: 469 EYSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQIT 528
           +YS+ +IM+S ID +  PEG++PWMG+    T  Y E+NN+GPGA T  RVKW G+K +T
Sbjct: 361 QYSKVVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLT 420

Query: 529 AKHALDFTPALFIR------GNPWIKPTGVPYSSGMM 560
           +  A ++ P  F         + WI  +GVPYS G M
Sbjct: 421 SNVAAEYYPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

BLAST of CmoCh06G015150 vs. TrEMBL
Match: A0A0A0L930_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G164510 PE=4 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 6.1e-245
Identity = 419/566 (74.03%), Postives = 480/566 (84.81%), Query Frame = 1

Query: 11  NKVAALVGLLSLFVVAMVN----GVN-------------GAEEHSESSSAIKTLCEPTDY 70
           NK AAL+G+ SLF+VAMV     GVN                E S S+ A+K LC+PTDY
Sbjct: 7   NKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTDY 66

Query: 71  RQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKALDDCS 130
           ++TCE+AL ++ T++ DPRELIKAGF+  V +IK AI NS TLKE ASDPMAK+ALD C 
Sbjct: 67  QETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQALDACG 126

Query: 131 ELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGE 190
           ELMDYAIDDL++SF +IT+ FD  K+DDYIEDL++WLSGALTYQ TC+DGFENVTGDTGE
Sbjct: 127 ELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTGDTGE 186

Query: 191 KMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEEVPSWVND 250
           KM  LL+ S+E+T N L MV EV S+++S     GLP +GR L T+ES E+ E PSWV D
Sbjct: 187 KMTKLLETSKELTINGLGMVSEVTSILTSF----GLPAIGRRLMTEESNEQREEPSWVRD 246

Query: 251 RRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQVMVD 310
           RRGLLQATGAN+KADAVVAKDGSGKYKT+T A+ DVPKKSNKTFVIY+K GVY+EQVMV+
Sbjct: 247 RRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVMVE 306

Query: 311 KKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQ 370
           K  T+VMMIGDGPTKTKITA +N+ DGT TFKTATVSVIG+NFI KD+ FENSAGAAKHQ
Sbjct: 307 KSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKHQ 366

Query: 371 AVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQNCKIF 430
           AVALRVQSDMS+FYNC+MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGN AVV QNCKI 
Sbjct: 367 AVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCKIL 426

Query: 431 VRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKEYSRT 490
           VRKP++NQ+CIVTAQGRTQ+KEPTAIILQNC ISS PD+FP+RHINKA+LGRPWK+YSRT
Sbjct: 427 VRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYSRT 486

Query: 491 IIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHAL 550
           IIM+SQIDDLIQPEGWLPW GNFALNTLFY+E NNRGPGA+T  RVKWKGIK+IT +HAL
Sbjct: 487 IIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEHAL 546

Query: 551 DFTPALFIRGNPWIKPTGVPYSSGMM 560
           DFT A FIRG+PWIKPTGVPY+SGMM
Sbjct: 547 DFTAARFIRGDPWIKPTGVPYTSGMM 568

BLAST of CmoCh06G015150 vs. TrEMBL
Match: W9S1C5_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_007196 PE=4 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 1.2e-195
Identity = 341/574 (59.41%), Postives = 435/574 (75.78%), Query Frame = 1

Query: 6   GGCKINKVAALVGLLSLFVVAMVN----GVNGAEEHSESSS-------------AIKTLC 65
           GG +  K+A ++G  +L +VAMV     G N +E  S+S++             AI+ +C
Sbjct: 10  GGQRKKKIA-IIGTSALILVAMVVAVAVGFNRSESDSQSAAPTSPPPSVSTTTKAIQAIC 69

Query: 66  EPTDYRQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKA 125
           +PTDYR+TCE +L  +  ++ DP+ELIKA F+  ++ I +AI NS TLKE A D    +A
Sbjct: 70  QPTDYRETCENSLTSAAGNTSDPKELIKAAFNVAIDHISAAIRNSSTLKELAKDSRTNQA 129

Query: 126 LDDCSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVT 185
           L +C EL+ YAIDDL  SFD+I   F++ K+D+Y++DL+IWLSGA+TY+ TCLDGFEN T
Sbjct: 130 LSNCDELLKYAIDDLKESFDRIGP-FEYEKLDEYVDDLKIWLSGAITYEQTCLDGFENTT 189

Query: 186 GDTGEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLP-PLGRELRTKESEEEEEV 245
           G+ G +MK +L+ SQE+T+N L MV E+ S+++S      LP  + R L + E  ++ E+
Sbjct: 190 GEAGLRMKAMLRSSQELTSNGLAMVTEISSILASY----NLPFNVSRRLLSSEDGDDTEL 249

Query: 246 PSWVND-RRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVY 305
            SW++D +R LLQATG  +KAD VVAKDGSGKY+T+ EA++D+PKK NKTFVIYIK+GVY
Sbjct: 250 -SWLSDGKRRLLQATGKTIKADVVVAKDGSGKYRTVNEALKDIPKKGNKTFVIYIKQGVY 309

Query: 306 EEQVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENS 365
           +EQVM  K  T+VM+IGDGPTKT+IT   NF DG  TFKTATVSVIG+NFI KD+ FENS
Sbjct: 310 KEQVMFAKSMTHVMIIGDGPTKTRITGSLNFIDGVQTFKTATVSVIGSNFIAKDIGFENS 369

Query: 366 AGAAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVV 425
           AG  KHQAVALRVQSDMSIFYNC MDGYQDTLY H HRQFYRDC ITGTIDFIFG++A V
Sbjct: 370 AGPQKHQAVALRVQSDMSIFYNCHMDGYQDTLYAHTHRQFYRDCVITGTIDFIFGDSAAV 429

Query: 426 LQNCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRP 485
            QNCK+ +RKPL+NQ CIVTAQGRT+++E TA++LQNC I+ DP Y PV+  NK YLGRP
Sbjct: 430 FQNCKMVIRKPLDNQNCIVTAQGRTERREATALVLQNCTITGDPAYLPVKDQNKGYLGRP 489

Query: 486 WKEYSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQ 545
           WKE+SRTIIM S+IDD+I PEGWLPW G+FALNT FY+E  NRGPG+    RVKW+GIK+
Sbjct: 490 WKEFSRTIIMNSRIDDVIAPEGWLPWAGDFALNTCFYTEILNRGPGSKLDGRVKWRGIKK 549

Query: 546 ITAKHALDFTPALFIRGNPWIKPTGVPYSSGMMN 561
           + A+ A+++TP  FIRG+ WIK  GVPY SGM+N
Sbjct: 550 VPAQRAVEYTPGRFIRGDWWIKAAGVPYVSGMLN 576

BLAST of CmoCh06G015150 vs. TrEMBL
Match: A0A061EQE7_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_021251 PE=4 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 2.5e-190
Identity = 336/571 (58.84%), Postives = 427/571 (74.78%), Query Frame = 1

Query: 9   KINKVAALVGLLSLFVVAMV-----------------NGVNGAEEHSESSSAIKTLCEPT 68
           K+ K  A++ + S+F+VAMV                  G     E S S+ AIK +C+PT
Sbjct: 121 KMGKKVAIISVCSVFLVAMVVAVAVGVSRSHGGRGNGGGATPTGEISTSTKAIKAICQPT 180

Query: 69  DYRQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKALDD 128
           DYR+TCE++L  S  ++ DP+ELIK GF   + +IK  I NS T+KE A DPMA++AL++
Sbjct: 181 DYRETCEKSL--SSANTTDPKELIKIGFQAAISEIKKVIANSSTVKEVAKDPMARQALEN 240

Query: 129 CSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDT 188
           C ELMDYAIDDL  SF+++ E FDFSK+D+YIE+L+IWL GA+TYQ TCLDGF N TG+ 
Sbjct: 241 CHELMDYAIDDLKNSFNQLGE-FDFSKLDEYIENLKIWLGGAITYQQTCLDGFMNTTGEA 300

Query: 189 GEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLGRELRTKESE--EEEEVPS 248
           G+KM+ LLK SQE+T+N L MV E+ S++ +L     +  +G E+ T +     E   P 
Sbjct: 301 GKKMQALLKTSQELTSNGLAMVSEISSILGNL----NIQNIG-EISTGQRRLFAENGFPM 360

Query: 249 WVN-DRRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEE 308
           WV+ ++R  ++ T A +K + VVAKDGSGKY +I EA+++VPK +  TFVIYIK GVY+E
Sbjct: 361 WVSSEQRSRIRQTPATIKPNVVVAKDGSGKYTSINEALQEVPKDNPTTFVIYIKAGVYKE 420

Query: 309 QVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAG 368
           QV+V K  T VM IGDG TKTKIT   N+ADGT TF+TATV V+G  FI KD+ FEN+AG
Sbjct: 421 QVIVTKSMTNVMFIGDGRTKTKITGDLNYADGTGTFRTATVGVVGDGFIAKDIGFENTAG 480

Query: 369 AAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQ 428
           A KHQAVALRVQSD SIFYNC+MDGYQDTLY H+HRQFYRDCTI+GTIDFIFG++A VLQ
Sbjct: 481 AIKHQAVALRVQSDQSIFYNCQMDGYQDTLYAHSHRQFYRDCTISGTIDFIFGDSATVLQ 540

Query: 429 NCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWK 488
           NCKI VRKPLENQ+CIVTAQGR +++E +A++LQNC IS DP+Y PV+  NKAYLGRPWK
Sbjct: 541 NCKIIVRKPLENQQCIVTAQGRIERREVSALVLQNCTISGDPEYLPVKDKNKAYLGRPWK 600

Query: 489 EYSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQIT 548
           E+SRT+IM+SQIDD+I PEGWLPW G+FALNTL+Y+EF NRGPGA    RV W+GIK+I 
Sbjct: 601 EFSRTLIMQSQIDDIIAPEGWLPWNGDFALNTLWYAEFGNRGPGAVQTHRVTWRGIKKIN 660

Query: 549 AKHALDFTPALFIRGNPWIKPTGVPYSSGMM 560
           A  A  FTP +F+RG+ WI   GVPY+ G++
Sbjct: 661 AAIARRFTPGVFLRGDDWIPKAGVPYTPGLI 683

BLAST of CmoCh06G015150 vs. TrEMBL
Match: F6H6V9_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_05s0077g01950 PE=4 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 2.0e-187
Identity = 329/574 (57.32%), Postives = 414/574 (72.13%), Query Frame = 1

Query: 12  KVAALVGLLSLFVVAMVNGVNGAEEHSESSS------------------AIKTLCEPTDY 71
           K  AL+G  S+ +VAMV G      H   SS                  AI+ +C+PTDY
Sbjct: 15  KKVALMGFSSILLVAMVVGTVVTVNHRNGSSSSATDDAQAGGGISTSVKAIQAICQPTDY 74

Query: 72  RQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKALDDCS 131
           ++ C  +L  +K ++ DP+EL++  F   + QI SA+ NS TL++   DP  K AL++C 
Sbjct: 75  KEACVNSLTSAKANTSDPKELVRTAFQVAINQISSALQNSTTLRDLEKDPRTKGALENCH 134

Query: 132 ELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGE 191
           ELMDYAIDDL  SF+K+   FD SK+DDY+EDL+IWLSGALTYQ TCLDGFEN TGD GE
Sbjct: 135 ELMDYAIDDLRNSFNKLG-VFDISKIDDYVEDLKIWLSGALTYQETCLDGFENTTGDAGE 194

Query: 192 KMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEE------- 251
           KMK LLK + E+T+N L M+ E+ SV+++L     +P + R L + ES + E        
Sbjct: 195 KMKALLKSAGELTSNGLAMIDEISSVLTNLQ----IPGISRRLLSDESGKGEYRSDEGGL 254

Query: 252 VPSWVN-DRRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGV 311
            PSW +  +R L QAT   +K + +VA+DGSGKYKTI EA+ ++PK  N TFV+Y+KEGV
Sbjct: 255 YPSWASVGQRKLFQATPDTIKPNVIVAQDGSGKYKTINEALVEIPKNGNTTFVLYVKEGV 314

Query: 312 YEEQVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFEN 371
           Y+EQV   K  T VM+IGDGPTKT I+   NF DG  TF+TATV+ +G+NF+ KD+ FEN
Sbjct: 315 YKEQVNFTKSMTNVMLIGDGPTKTTISGSLNFIDGIGTFRTATVAAVGSNFMAKDIGFEN 374

Query: 372 SAGAAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAV 431
           +AGA+KHQAVALRV SDM+IFYNCRMDGYQDTLY HAHRQFYRDCTITGTIDFIFG++AV
Sbjct: 375 NAGASKHQAVALRVGSDMAIFYNCRMDGYQDTLYVHAHRQFYRDCTITGTIDFIFGDSAV 434

Query: 432 VLQNCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGR 491
           V QNC I VRKPL+NQ+CIVTAQGR +++EPT I+LQNC IS+  DY P +   K+YLGR
Sbjct: 435 VFQNCLILVRKPLDNQQCIVTAQGRNERREPTGIVLQNCTISAADDYIPFKTKFKSYLGR 494

Query: 492 PWKEYSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIK 551
           PWK +SRTIIM+SQIDDLI PEGWLPWMG+F LNT FY+E+ NRGP ++T  RV W+GIK
Sbjct: 495 PWKAFSRTIIMQSQIDDLISPEGWLPWMGDFGLNTCFYAEYGNRGPASATTSRVTWRGIK 554

Query: 552 QITAKHALDFTPALFIRGNPWIKPTGVPYSSGMM 560
           QIT +H  DFT   FI G+ W+  +GVPY+S MM
Sbjct: 555 QITGQHVNDFTVGRFISGHLWLGASGVPYTSDMM 583

BLAST of CmoCh06G015150 vs. TrEMBL
Match: A0A0A0KD24_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G318700 PE=4 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.3e-186
Identity = 333/575 (57.91%), Postives = 415/575 (72.17%), Query Frame = 1

Query: 7   GCKINKVAALVGLLSLFVVAMVN----GVNGA---EEHSE-------------SSSAIKT 66
           G K  K  A++ + SL +VAMV     GVN A   EEH +             S+ AI+ 
Sbjct: 10  GDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGDDNHGSETTPEITSSTKAIQA 69

Query: 67  LCEPTDYRQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAK 126
           +C+PTDY++TCE++L     ++ DP+EL+K GF      +  AI NS TLKE A DP   
Sbjct: 70  ICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKELAKDPRTN 129

Query: 127 KALDDCSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFEN 186
           +AL +C EL++YAIDDL  SF++I  TF  SK+DD++ DL+IWLSGALTY+ TCLDGFEN
Sbjct: 130 QALQNCRELLEYAIDDLNQSFERIG-TFQMSKLDDFVADLKIWLSGALTYEQTCLDGFEN 189

Query: 187 VTGDTGEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPL-GRELRTKESEEEE 246
            TGD G +M+  LK +Q+MT N L +V E+ +++ SL     LP + GR L     +++ 
Sbjct: 190 TTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQ----LPGMSGRRLL---EDDDR 249

Query: 247 EVPSWVND-RRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEG 306
           E+PSWV+D +R L+QA  A +K D VVA+DGSGKYKTI  A+ DVP KSNKTFVI++K G
Sbjct: 250 EMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIHVKAG 309

Query: 307 VYEEQVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFE 366
           +Y+E V++ K  T++ M GDGPTKT +T   NF DG  TFKTAT S IG NF  +D+ FE
Sbjct: 310 IYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDMGFE 369

Query: 367 NSAGAAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAA 426
           N+AGAAKHQAVALRVQSD SIF+NC++DGYQDTLY HAHRQFYRDCTI+GTIDF+FGNAA
Sbjct: 370 NTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFGNAA 429

Query: 427 VVLQNCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLG 486
              QNCK+ VRKPL+NQ+CIVTA GR  +KEPTA+I Q+C    DP Y P + INKAYLG
Sbjct: 430 TNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINKAYLG 489

Query: 487 RPWKEYSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGI 546
           RPWKEYSRTII+ S IDD+IQPEGWLPWMG+F LNTLFY+E  N+G GA    RVKW+GI
Sbjct: 490 RPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVKWRGI 549

Query: 547 KQITAKHALDFTPALFIRGNPWIKPTGVPYSSGMM 560
           K IT +HA DFTP  FI G+ WI   G+PYSSGMM
Sbjct: 550 KHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

BLAST of CmoCh06G015150 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 565.8 bits (1457), Expect = 2.9e-161
Identity = 282/571 (49.39%), Postives = 383/571 (67.08%), Query Frame = 1

Query: 3   YDGGGCKINKVAALVGLLSLFVVAMVNGVN-----------GAEEHSESSSAIKTLCEPT 62
           YD    K  K   ++ + S+ +++MV  V            G EE + S  AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 63  DYRQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKALDD 122
           DY++TCE+ L K   D+ DP EL+K  F+  ++QI      S+T+ E   DP AK ALD 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 123 CSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDT 182
           C ELMDYAI +L  SF+++ + F+F K+D+ +  LRIWLS  ++++ TCLDGF+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEELGK-FEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 183 GEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLG-RELRTKESEEEEEVPSW 242
           GE +K  LK + ++T N L MV E+ + +  +     +P +  R L +      +E PSW
Sbjct: 185 GETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQ----IPEMNSRRLLS------QEFPSW 244

Query: 243 VNDR-RGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQ 302
           ++ R R LL A  + VK D VVA+DGSG+YKTI EA+  VPKK N TFV++IKEG+Y+E 
Sbjct: 245 MDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEY 304

Query: 303 VMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAGA 362
           V V++  T+++ IGDGP KT I+  +++ DG +T+KTATV+++G +FI K++ FEN+AGA
Sbjct: 305 VQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGA 364

Query: 363 AKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQN 422
            KHQAVA+RV +D SIFYNC+ DGYQDTLY H+HRQFYRDCTI+GTIDF+FG+AA V QN
Sbjct: 365 IKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQN 424

Query: 423 CKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKE 482
           C + VRKPL NQ C +TA GR   +E T  +LQ C I  +PDY  V+  +K YLGRPWKE
Sbjct: 425 CTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKE 484

Query: 483 YSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITA 542
           YSRTIIM + I D + PEGW PW+G F LNTLFYSE  N GPGA+   RV W GIK+++ 
Sbjct: 485 YSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSD 544

Query: 543 KHALDFTPALFIRGNPWIKPTGVPYSSGMMN 561
           +  L FTPA +I+G+ WI   GVPY  G+ +
Sbjct: 545 EEILKFTPAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of CmoCh06G015150 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 564.3 bits (1453), Expect = 8.4e-161
Identity = 278/553 (50.27%), Postives = 382/553 (69.08%), Query Frame = 1

Query: 19  LLSLFVVAMVNGVN---------GAEEHSESSSAIKTLCEPTDYRQTCEEALVKSKTDSQ 78
           LL   VVA+  GV+         G  E + S  A+K +C PTDYR+TCE+ L+K+  ++ 
Sbjct: 24  LLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNGKNTT 83

Query: 79  DPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKALDDCSELMDYAIDDLLLSFDK 138
           DP EL+K  F+  ++QI  A   S+T+ E   D   + ALD C ELMDYA+D+L  SF++
Sbjct: 84  DPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEE 143

Query: 139 ITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGEKMKHLLKLSQEMTANA 198
           + + F+F  +D+ + +LRIWLS A++++ TCL+GF+   G+ GE MK  LK + E+T N 
Sbjct: 144 LGK-FEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNG 203

Query: 199 LEMVGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEEVPSWVNDR-RGLLQATGA--NVK 258
           L ++ E+ + V  +     +P L       E       PSWV+ R R LLQA  A  +VK
Sbjct: 204 LAIISEMSNFVGQMQ----IPGLNSRRLLAEG-----FPSWVDQRGRKLLQAAAAYSDVK 263

Query: 259 ADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQVMVDKKTTYVMMIGDGP 318
            D VVA+DGSG+YKTI EA++ VPKK N TFV++IK G+Y+E V V+K  ++++ IGDGP
Sbjct: 264 PDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGP 323

Query: 319 TKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDMSIF 378
            KT I+  +N+ DG +T++TATV+++G  FI K++ FEN+AGA KHQAVA+RVQSD SIF
Sbjct: 324 DKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIF 383

Query: 379 YNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQNCKIFVRKPLENQKCIVT 438
           +NCR DGYQDTLYTH+HRQF+RDCTI+GTIDF+FG+AA V QNC + VRKPL NQ C +T
Sbjct: 384 FNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPIT 443

Query: 439 AQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKEYSRTIIMESQIDDLIQP 498
           A GR   +E T  + Q C I+ +PDY  V+  +KAYLGRPWKEYSRTIIM + I D +QP
Sbjct: 444 AHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQP 503

Query: 499 EGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHALDFTPALFIRGNPW 558
           +GW PW+G+F L TLFYSE  N GPG++  +RV W GIK ++ +  L FTPA +I+G+ W
Sbjct: 504 QGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDW 563

Query: 559 IKPTGVPYSSGMM 560
           I   GVPY++G++
Sbjct: 564 IPGKGVPYTTGLL 566

BLAST of CmoCh06G015150 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 553.1 bits (1424), Expect = 1.9e-157
Identity = 274/551 (49.73%), Postives = 374/551 (67.88%), Query Frame = 1

Query: 16  LVGLLSLFVVAMVNGVNGAEEHSE---------SSSAIKTLCEPTDYRQTCEEALVKSKT 75
           +  LL L VVA+    +    HSE         +++A++ +C PTDY++TC  +L+K+  
Sbjct: 18  ITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSLMKASP 77

Query: 76  DSQDPRELIKAGFHYVVEQIKSAITNSKT--LKETASDPMAKKALDDCSELMDYAIDDLL 135
           DS  P +LIK GF+  +  I+ +I  +      + A+D   K AL+ C +LM+ A DDL 
Sbjct: 78  DSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDATDDLK 137

Query: 136 LSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGEKMKHLLKLSQE 195
              D   + F   +++D++EDLR+WLSG++ YQ TC+D FE       + M+ + K S+E
Sbjct: 138 KCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTSRE 197

Query: 196 MTANALEMVGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEEVPSWVNDRRGLLQATGAN 255
           +T+N L M+  + +++           LG+  R K    E+ +PSWV      L AT   
Sbjct: 198 LTSNGLAMITNISNLLGEFNVTGVTGDLGKYAR-KLLSAEDGIPSWVGPNTRRLMATKGG 257

Query: 256 VKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQVMVDKKTTYVMMIGD 315
           VKA+ VVA DGSG+YKTI EA+  VPK + K FVIYIK+GVY E+V V KK T+V  IGD
Sbjct: 258 VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGD 317

Query: 316 GPTKTKITARENFADG-TSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDM 375
           GPTKTKIT   N+  G   T+ TATV++ G NF  K++ FEN+AG   HQAVALRV +D+
Sbjct: 318 GPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADL 377

Query: 376 SIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQNCKIFVRKPLENQKC 435
           ++FYNC++DGYQDTLY H+HRQF+RDCT++GT+DFIFG+  VVLQNC I VRKP+++Q C
Sbjct: 378 AVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSC 437

Query: 436 IVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKEYSRTIIMESQIDDL 495
           ++TAQGR+ K+E T ++LQNC I+ +P Y PV+ INKAYLGRPWKE+SRTIIM + IDD+
Sbjct: 438 MITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTIIMGTTIDDV 497

Query: 496 IQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHALDFTPALFIRG 555
           I P GWLPW G+FALNTL+Y+E+ N GPG++   RVKW GIK+++ K AL FTPA F+RG
Sbjct: 498 IDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRG 557

BLAST of CmoCh06G015150 vs. TAIR10
Match: AT3G06830.1 (AT3G06830.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 490.0 bits (1260), Expect = 2.0e-138
Identity = 248/549 (45.17%), Postives = 366/549 (66.67%), Query Frame = 1

Query: 16  LVGLLSLFVVAMVNGVNGAEEH-SESSSAIKTLCEPTDYRQTCEEALVKSKTDSQDPREL 75
           ++ ++S+ VV   +     E H  +++ A++ +C PTD++ TC  +L+ +  DS DP +L
Sbjct: 22  VIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTCVNSLMGASPDSDDPVDL 81

Query: 76  IKAGFHYVVEQIKSAITN-SKTLKETAS-DPMAKKALDDCSELMDYAIDDLLLSFDKITE 135
           IK GF   ++ I  ++   S  +K  A  +P AK A + C +LM  AIDDL    D    
Sbjct: 82  IKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKLMIDAIDDLKKCMD---H 141

Query: 136 TFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGEKMKHLLKLSQEMTANALEM 195
            F   +++ ++EDLR+WLSG++ +Q TC+D F  +  +  + M  + K S+E+++N+L M
Sbjct: 142 GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDMLKIFKTSRELSSNSLAM 201

Query: 196 VGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEEVPSWVN-DRRGLLQATGAN---VKAD 255
           V  + +++ +     GL     +   K    E+ +P+WV  + R L+ A G     VKA+
Sbjct: 202 VTRISTLIPNS-NLTGLTGALAKYARKLLSTEDSIPTWVGPEARRLMAAQGGGPGPVKAN 261

Query: 256 AVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQVMVDKKTTYVMMIGDGPTK 315
           AVVA+DG+G++KTIT+A+  VPK +   F+I+IKEG+Y+E+V V KK  +V  IGDGP K
Sbjct: 262 AVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNK 321

Query: 316 TKITARENFADG-TSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQAVALRVQSDMSIFY 375
           T IT   NF  G   TF TAT+++ G +F  K++  EN+AG    QAVALRV +D ++F+
Sbjct: 322 TLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFH 381

Query: 376 NCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQNCKIFVRKPLENQKCIVTA 435
           +C++DG+QDTLY H+HRQFYRDCT++GT+DFIFG+A  +LQNCKI VRKP + Q C+VTA
Sbjct: 382 SCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTA 441

Query: 436 QGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKEYSRTIIMESQIDDLIQPE 495
           QGR+  +E T ++L  C I+ DP Y P++ +NKAYLGRPWKE+SRTIIM++ IDD+I P 
Sbjct: 442 QGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPA 501

Query: 496 GWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHALDFTPALFIRGNPWI 555
           GWLPW G+FAL TL+Y+E  N GPG++   RVKW GIK++T + AL +T   F+RG+ WI
Sbjct: 502 GWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWI 561

Query: 556 KPTGVPYSS 557
             T VPY++
Sbjct: 562 PQTQVPYTA 566

BLAST of CmoCh06G015150 vs. TAIR10
Match: AT2G26450.1 (AT2G26450.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 412.5 bits (1059), Expect = 4.1e-115
Identity = 221/527 (41.94%), Postives = 320/527 (60.72%), Query Frame = 1

Query: 39  ESSSAIKTLCEPTDYRQTCEEALVKSKTDS----QDPRELIKAGFHYVVEQIKSAITNSK 98
           +    I+TLC  T Y Q CE+ L K++TD      +P   +K+    V E +   +    
Sbjct: 104 QGDKIIQTLCSSTLYMQICEKTL-KNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVL 163

Query: 99  TLKETASDPMAKKALDDCSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGAL 158
           +LK    D   K A++ C  L++ A ++ + S +KI  T + +  +  + DL  WLS  +
Sbjct: 164 SLKTENQDD--KDAIEQCKLLVEDAKEETVASLNKINVT-EVNSFEKVVPDLESWLSAVM 223

Query: 159 TYQVTCLDGFENVTGDTGEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLGR 218
           +YQ TCLDGFE   G+   ++K  +  SQ +T+N+L ++      +S +           
Sbjct: 224 SYQETCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENLSPV----------- 283

Query: 219 ELRTKESEEEEEVPSWV-NDRRGLLQATGAN-VKADAVVAKDGSGKYKTITEAIRDVPKK 278
            ++  E    +++PSWV ND R +L+A     +K +A VAKDGSG + TI +A+R +P+K
Sbjct: 284 -MKVVERHLLDDIPSWVSNDDRRMLRAVDVKALKPNATVAKDGSGDFTTINDALRAMPEK 343

Query: 279 SNKTFVIYIKEGVYEEQVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVI 338
               ++IY+K+G+Y+E V VDKK   + M+GDG  KT +T  ++ A    TF TAT    
Sbjct: 344 YEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQ 403

Query: 339 GTNFIGKDLWFENSAGAAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTI 398
           G  F+ + + F N+AG   HQAVA+RVQSD SIF NCR +GYQDTLY + HRQ+YR C I
Sbjct: 404 GEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVI 463

Query: 399 TGTIDFIFGNAAVVLQNCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDY 458
            GTIDFIFG+AA + QNC IF+RK L  QK  VTAQGR  K + T  ++ NC I+++ D 
Sbjct: 464 VGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDL 523

Query: 459 FPVRHINKAYLGRPWKEYSRTIIMESQIDDLIQPEGWLPWM-GNFALNTLFYSEFNNRGP 518
            PV+   K+YLGRPWK YSRTIIMES+I+++I P GWL W   +FA++TL+Y+E+NN+G 
Sbjct: 524 KPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGS 583

Query: 519 GASTKDRVKWKGIKQITAKHALDFTPALFIRGNPWIKPTGVPYSSGM 559
              T  RVKW G K I  + AL++T   F++G+ WI  +G P   G+
Sbjct: 584 SGDTTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

BLAST of CmoCh06G015150 vs. NCBI nr
Match: gi|659076869|ref|XP_008438906.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 856.3 bits (2211), Expect = 3.0e-245
Identity = 422/573 (73.65%), Postives = 486/573 (84.82%), Query Frame = 1

Query: 4   DGGGCKINKVAALVGLLSLFVVAMV----------------NGVNGAE-EHSESSSAIKT 63
           DGG    NK AAL+G+ SLF+VAMV                +G +GA  E S S+ A++ 
Sbjct: 3   DGGK---NKKAALIGVSSLFLVAMVVAVAVGVNRKNPTTDSSGGDGATAEISTSTKAVQA 62

Query: 64  LCEPTDYRQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAK 123
           LC+PTDY++TCE+AL ++ T++ DPRELIK+GFH  V +IK AI NS  LKE ASDPMAK
Sbjct: 63  LCQPTDYKETCEKALSEAGTNTSDPRELIKSGFHVAVNEIKWAIGNSTQLKEAASDPMAK 122

Query: 124 KALDDCSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFEN 183
           +ALD C ELMDYAIDDL++SFD+IT+ FD  K++DY+EDL+IWLSGALTYQ TC+DGFEN
Sbjct: 123 QALDACGELMDYAIDDLVISFDRITDNFDVQKLNDYVEDLKIWLSGALTYQETCIDGFEN 182

Query: 184 VTGDTGEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEE 243
           VTGDTGEKM  LLK S+E+T+N L+MV EV S+++S     GLP +GR L T+ES ++EE
Sbjct: 183 VTGDTGEKMAKLLKTSKELTSNGLDMVSEVNSIMTSF----GLPAVGRRLLTEESNQQEE 242

Query: 244 VPSWVNDRRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVY 303
            PSWV DRRGLLQATGA VKAD VVAKDGSGKYKT+TEA+ DVPKKSNKTFVI++K GVY
Sbjct: 243 EPSWVGDRRGLLQATGATVKADVVVAKDGSGKYKTVTEALNDVPKKSNKTFVIHVKAGVY 302

Query: 304 EEQVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENS 363
           +EQVMVDK  T+VMMIGDGPTKTKITA +N+ DGT TFKTATVSVIG+NFI KD+ FENS
Sbjct: 303 QEQVMVDKSMTWVMMIGDGPTKTKITAGKNYVDGTPTFKTATVSVIGSNFIAKDIGFENS 362

Query: 364 AGAAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVV 423
           AGAAKHQAVALRVQSDMSIFYNC+MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAA V
Sbjct: 363 AGAAKHQAVALRVQSDMSIFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAAV 422

Query: 424 LQNCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRP 483
            QNCKI VRKP++NQ+CIVTAQGRTQ+KEPTAIILQNC ISSDP +FPVRHINKAYLGRP
Sbjct: 423 FQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSDPTFFPVRHINKAYLGRP 482

Query: 484 WKEYSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQ 543
           WK+YSRTIIM+ QIDDLIQPEGWLPWMG+FAL+TLFY+E NNRGPGA+T  RVKWKGIK+
Sbjct: 483 WKQYSRTIIMQCQIDDLIQPEGWLPWMGDFALHTLFYAEINNRGPGAATDKRVKWKGIKK 542

Query: 544 ITAKHALDFTPALFIRGNPWIKPTGVPYSSGMM 560
           IT +HALDFT   FIRG+PWIKPTGVPYSSGMM
Sbjct: 543 ITPQHALDFTVGRFIRGDPWIKPTGVPYSSGMM 568

BLAST of CmoCh06G015150 vs. NCBI nr
Match: gi|778678954|ref|XP_004134493.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 854.7 bits (2207), Expect = 8.8e-245
Identity = 419/566 (74.03%), Postives = 480/566 (84.81%), Query Frame = 1

Query: 11  NKVAALVGLLSLFVVAMVN----GVN-------------GAEEHSESSSAIKTLCEPTDY 70
           NK AAL+G+ SLF+VAMV     GVN                E S S+ A+K LC+PTDY
Sbjct: 7   NKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTDY 66

Query: 71  RQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKALDDCS 130
           ++TCE+AL ++ T++ DPRELIKAGF+  V +IK AI NS TLKE ASDPMAK+ALD C 
Sbjct: 67  QETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQALDACG 126

Query: 131 ELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDTGE 190
           ELMDYAIDDL++SF +IT+ FD  K+DDYIEDL++WLSGALTYQ TC+DGFENVTGDTGE
Sbjct: 127 ELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTGDTGE 186

Query: 191 KMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLGRELRTKESEEEEEVPSWVND 250
           KM  LL+ S+E+T N L MV EV S+++S     GLP +GR L T+ES E+ E PSWV D
Sbjct: 187 KMTKLLETSKELTINGLGMVSEVTSILTSF----GLPAIGRRLMTEESNEQREEPSWVRD 246

Query: 251 RRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEEQVMVD 310
           RRGLLQATGAN+KADAVVAKDGSGKYKT+T A+ DVPKKSNKTFVIY+K GVY+EQVMV+
Sbjct: 247 RRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVMVE 306

Query: 311 KKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAGAAKHQ 370
           K  T+VMMIGDGPTKTKITA +N+ DGT TFKTATVSVIG+NFI KD+ FENSAGAAKHQ
Sbjct: 307 KSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKHQ 366

Query: 371 AVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQNCKIF 430
           AVALRVQSDMS+FYNC+MDGYQDTLYTHAHRQFYRDCTITGTIDFIFGN AVV QNCKI 
Sbjct: 367 AVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCKIL 426

Query: 431 VRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWKEYSRT 490
           VRKP++NQ+CIVTAQGRTQ+KEPTAIILQNC ISS PD+FP+RHINKA+LGRPWK+YSRT
Sbjct: 427 VRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYSRT 486

Query: 491 IIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQITAKHAL 550
           IIM+SQIDDLIQPEGWLPW GNFALNTLFY+E NNRGPGA+T  RVKWKGIK+IT +HAL
Sbjct: 487 IIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEHAL 546

Query: 551 DFTPALFIRGNPWIKPTGVPYSSGMM 560
           DFT A FIRG+PWIKPTGVPY+SGMM
Sbjct: 547 DFTAARFIRGDPWIKPTGVPYTSGMM 568

BLAST of CmoCh06G015150 vs. NCBI nr
Match: gi|1009163283|ref|XP_015899883.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 711.4 bits (1835), Expect = 1.2e-201
Identity = 354/574 (61.67%), Postives = 437/574 (76.13%), Query Frame = 1

Query: 4   DGGGCKINKVAALVGLLSLFVVAMV--------NGVNGAEEHSESSS------AIKTLCE 63
           DGG  K  KVA ++G  +L +VAMV         G +  ++HS S S      A+K +C 
Sbjct: 8   DGGNGKKKKVA-IMGASALILVAMVVAVAVGVHKGGSDDDDHSSSGSISTSSKAVKQICA 67

Query: 64  PTDYRQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKAL 123
           PTDYR+TCE +L  S  +S DP+ELIKAGF   V+ ++ AI NS TLKE A DP A +AL
Sbjct: 68  PTDYRETCETSL--SSVNSTDPKELIKAGFQVAVQHLRVAIENSTTLKELAKDPRANQAL 127

Query: 124 DDCSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTG 183
            +C ELMDYAIDD+  SF+++   FD SK+++Y+EDL++WL GA+TY+ TCLDGFEN TG
Sbjct: 128 SNCEELMDYAIDDIQDSFNRLG-AFDISKLEEYVEDLKVWLGGAVTYEQTCLDGFENTTG 187

Query: 184 DTGEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLP--PLGRELRTKESEEEEEV 243
           D G++MK +LK +QE+T+N L MV E+ +V++S    L LP  P       +    EEE 
Sbjct: 188 DAGDRMKAILKTAQELTSNGLAMVTEIYNVLNS---NLNLPNAPSTPNPPHRRLLNEEET 247

Query: 244 PSWVND-RRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVY 303
           PSWVND +R LL A+ A++K D VVAKDGSGKYKT+ EA++D+PK  N TFVIYIK GVY
Sbjct: 248 PSWVNDGKRKLLAASPADLKPDVVVAKDGSGKYKTVNEALKDIPKYGNNTFVIYIKAGVY 307

Query: 304 EEQVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENS 363
           EE V++DK  T+VMMIGDGPTK+ IT  +N+ DGT TFKTATV++IG NF  KD+ FENS
Sbjct: 308 EETVIIDKTMTHVMMIGDGPTKSVITGSKNYVDGTLTFKTATVTIIGGNFTAKDMGFENS 367

Query: 364 AGAAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVV 423
           AG  KHQAVALRVQSD+S+FYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFG+A  V
Sbjct: 368 AGPEKHQAVALRVQSDLSLFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGDAGAV 427

Query: 424 LQNCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRP 483
            QNC++ VRKPL+NQ CIVTAQGR+ ++ P+AIILQNC IS DP Y PV+ +NKAYLGRP
Sbjct: 428 FQNCQMIVRKPLDNQNCIVTAQGRSDRRTPSAIILQNCTISGDPLYMPVKDVNKAYLGRP 487

Query: 484 WKEYSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQ 543
           WKE+SRTIIM+SQIDD+IQP+GWLPW G FA+N+ FY+EFNNRG GA    RVKWKGIK 
Sbjct: 488 WKEFSRTIIMQSQIDDIIQPDGWLPWAGTFAINSCFYTEFNNRGAGADMSKRVKWKGIKT 547

Query: 544 ITAKHALDFTPALFIRGNPWIKPTGVPYSSGMMN 561
           IT +HA+DFTP  F+ G+ WIK TG+PY SGM+N
Sbjct: 548 ITPQHAVDFTPGRFLGGDRWIKATGIPYVSGMIN 574

BLAST of CmoCh06G015150 vs. NCBI nr
Match: gi|703132462|ref|XP_010105131.1| (Putative pectinesterase/pectinesterase inhibitor 28 [Morus notabilis])

HSP 1 Score: 691.0 bits (1782), Expect = 1.7e-195
Identity = 341/574 (59.41%), Postives = 435/574 (75.78%), Query Frame = 1

Query: 6   GGCKINKVAALVGLLSLFVVAMVN----GVNGAEEHSESSS-------------AIKTLC 65
           GG +  K+A ++G  +L +VAMV     G N +E  S+S++             AI+ +C
Sbjct: 10  GGQRKKKIA-IIGTSALILVAMVVAVAVGFNRSESDSQSAAPTSPPPSVSTTTKAIQAIC 69

Query: 66  EPTDYRQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKA 125
           +PTDYR+TCE +L  +  ++ DP+ELIKA F+  ++ I +AI NS TLKE A D    +A
Sbjct: 70  QPTDYRETCENSLTSAAGNTSDPKELIKAAFNVAIDHISAAIRNSSTLKELAKDSRTNQA 129

Query: 126 LDDCSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVT 185
           L +C EL+ YAIDDL  SFD+I   F++ K+D+Y++DL+IWLSGA+TY+ TCLDGFEN T
Sbjct: 130 LSNCDELLKYAIDDLKESFDRIGP-FEYEKLDEYVDDLKIWLSGAITYEQTCLDGFENTT 189

Query: 186 GDTGEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLP-PLGRELRTKESEEEEEV 245
           G+ G +MK +L+ SQE+T+N L MV E+ S+++S      LP  + R L + E  ++ E+
Sbjct: 190 GEAGLRMKAMLRSSQELTSNGLAMVTEISSILASY----NLPFNVSRRLLSSEDGDDTEL 249

Query: 246 PSWVND-RRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVY 305
            SW++D +R LLQATG  +KAD VVAKDGSGKY+T+ EA++D+PKK NKTFVIYIK+GVY
Sbjct: 250 -SWLSDGKRRLLQATGKTIKADVVVAKDGSGKYRTVNEALKDIPKKGNKTFVIYIKQGVY 309

Query: 306 EEQVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENS 365
           +EQVM  K  T+VM+IGDGPTKT+IT   NF DG  TFKTATVSVIG+NFI KD+ FENS
Sbjct: 310 KEQVMFAKSMTHVMIIGDGPTKTRITGSLNFIDGVQTFKTATVSVIGSNFIAKDIGFENS 369

Query: 366 AGAAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVV 425
           AG  KHQAVALRVQSDMSIFYNC MDGYQDTLY H HRQFYRDC ITGTIDFIFG++A V
Sbjct: 370 AGPQKHQAVALRVQSDMSIFYNCHMDGYQDTLYAHTHRQFYRDCVITGTIDFIFGDSAAV 429

Query: 426 LQNCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRP 485
            QNCK+ +RKPL+NQ CIVTAQGRT+++E TA++LQNC I+ DP Y PV+  NK YLGRP
Sbjct: 430 FQNCKMVIRKPLDNQNCIVTAQGRTERREATALVLQNCTITGDPAYLPVKDQNKGYLGRP 489

Query: 486 WKEYSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQ 545
           WKE+SRTIIM S+IDD+I PEGWLPW G+FALNT FY+E  NRGPG+    RVKW+GIK+
Sbjct: 490 WKEFSRTIIMNSRIDDVIAPEGWLPWAGDFALNTCFYTEILNRGPGSKLDGRVKWRGIKK 549

Query: 546 ITAKHALDFTPALFIRGNPWIKPTGVPYSSGMMN 561
           + A+ A+++TP  FIRG+ WIK  GVPY SGM+N
Sbjct: 550 VPAQRAVEYTPGRFIRGDWWIKAAGVPYVSGMLN 576

BLAST of CmoCh06G015150 vs. NCBI nr
Match: gi|590661338|ref|XP_007035646.1| (Plant invertase/pectin methylesterase inhibitor superfamily, putative [Theobroma cacao])

HSP 1 Score: 673.3 bits (1736), Expect = 3.6e-190
Identity = 336/571 (58.84%), Postives = 427/571 (74.78%), Query Frame = 1

Query: 9   KINKVAALVGLLSLFVVAMV-----------------NGVNGAEEHSESSSAIKTLCEPT 68
           K+ K  A++ + S+F+VAMV                  G     E S S+ AIK +C+PT
Sbjct: 121 KMGKKVAIISVCSVFLVAMVVAVAVGVSRSHGGRGNGGGATPTGEISTSTKAIKAICQPT 180

Query: 69  DYRQTCEEALVKSKTDSQDPRELIKAGFHYVVEQIKSAITNSKTLKETASDPMAKKALDD 128
           DYR+TCE++L  S  ++ DP+ELIK GF   + +IK  I NS T+KE A DPMA++AL++
Sbjct: 181 DYRETCEKSL--SSANTTDPKELIKIGFQAAISEIKKVIANSSTVKEVAKDPMARQALEN 240

Query: 129 CSELMDYAIDDLLLSFDKITETFDFSKMDDYIEDLRIWLSGALTYQVTCLDGFENVTGDT 188
           C ELMDYAIDDL  SF+++ E FDFSK+D+YIE+L+IWL GA+TYQ TCLDGF N TG+ 
Sbjct: 241 CHELMDYAIDDLKNSFNQLGE-FDFSKLDEYIENLKIWLGGAITYQQTCLDGFMNTTGEA 300

Query: 189 GEKMKHLLKLSQEMTANALEMVGEVKSVVSSLWEGLGLPPLGRELRTKESE--EEEEVPS 248
           G+KM+ LLK SQE+T+N L MV E+ S++ +L     +  +G E+ T +     E   P 
Sbjct: 301 GKKMQALLKTSQELTSNGLAMVSEISSILGNL----NIQNIG-EISTGQRRLFAENGFPM 360

Query: 249 WVN-DRRGLLQATGANVKADAVVAKDGSGKYKTITEAIRDVPKKSNKTFVIYIKEGVYEE 308
           WV+ ++R  ++ T A +K + VVAKDGSGKY +I EA+++VPK +  TFVIYIK GVY+E
Sbjct: 361 WVSSEQRSRIRQTPATIKPNVVVAKDGSGKYTSINEALQEVPKDNPTTFVIYIKAGVYKE 420

Query: 309 QVMVDKKTTYVMMIGDGPTKTKITARENFADGTSTFKTATVSVIGTNFIGKDLWFENSAG 368
           QV+V K  T VM IGDG TKTKIT   N+ADGT TF+TATV V+G  FI KD+ FEN+AG
Sbjct: 421 QVIVTKSMTNVMFIGDGRTKTKITGDLNYADGTGTFRTATVGVVGDGFIAKDIGFENTAG 480

Query: 369 AAKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAVVLQ 428
           A KHQAVALRVQSD SIFYNC+MDGYQDTLY H+HRQFYRDCTI+GTIDFIFG++A VLQ
Sbjct: 481 AIKHQAVALRVQSDQSIFYNCQMDGYQDTLYAHSHRQFYRDCTISGTIDFIFGDSATVLQ 540

Query: 429 NCKIFVRKPLENQKCIVTAQGRTQKKEPTAIILQNCVISSDPDYFPVRHINKAYLGRPWK 488
           NCKI VRKPLENQ+CIVTAQGR +++E +A++LQNC IS DP+Y PV+  NKAYLGRPWK
Sbjct: 541 NCKIIVRKPLENQQCIVTAQGRIERREVSALVLQNCTISGDPEYLPVKDKNKAYLGRPWK 600

Query: 489 EYSRTIIMESQIDDLIQPEGWLPWMGNFALNTLFYSEFNNRGPGASTKDRVKWKGIKQIT 548
           E+SRT+IM+SQIDD+I PEGWLPW G+FALNTL+Y+EF NRGPGA    RV W+GIK+I 
Sbjct: 601 EFSRTLIMQSQIDDIIAPEGWLPWNGDFALNTLWYAEFGNRGPGAVQTHRVTWRGIKKIN 660

Query: 549 AKHALDFTPALFIRGNPWIKPTGVPYSSGMM 560
           A  A  FTP +F+RG+ WI   GVPY+ G++
Sbjct: 661 AAIARRFTPGVFLRGDDWIPKAGVPYTPGLI 683