CmoCh06G005120 (gene) Cucurbita moschata (Rifu)

NameCmoCh06G005120
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionEukaryotic aspartyl protease family protein
LocationCmo_Chr06 : 2461472 .. 2462845 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTCCTCCCCCGCTCTGTTTCTCATACATTCTCCTCCTCTTCTCCGTTTCCGCCATTGTCGACGCCAACGCCAACTCCATCACTCTCCCTCTCTCCGCCTTACCCCACCCTTCTTCCTCAGATCCACTCCAAAATCTCAATTTCCTCGCCTCTGCTTCCCAGAACAGAGCCCATCAAATCAAAACCCCAAAATCCAACTCCGTTTCCAAATCCCCTCTCTCCCCCCATAGCTATGGAGCTTACTCCGCTCCACTCAGCTTCGGTACCCCACCCCAGACTCTCCATTTGATATTTGATACAGGTAGCAGCCTCGTTTGGTTTCCTTGTACTTCCAAATATCTCTGTTCCCAATGTTCGTTTCCCAAAATAGATCCTCTCCGGATCCCAAGATTTGTCCCCAAATTGTCCTCTTCCTCCAAGCTTGTCGGCTGCCAGAATCCAAAATGTGCTTGGGTTTTCGGCCCTGACGTGAAGTCTCAGTGCCGGAATTGTAACCCGAAAACAGAGAACTGTACCCAAACATGTCCTGCTTATGCTGTTCAGTATGGCTCTGGTTCCACGGCCGGGCTTTTGCTATCGGAGACGCTGGATTTTCCCGATCAAAAAATCCCCAATTTCGTTGTGGGGTGTTCGTTTCTTTCGATTCATCAGCCTTCTGGAATCGCTGGATTCGGCCGAGGATCCGAATCGCTTCCGTCGCAAATGGGTCTGAAGAAATTTGCCTACTGTCTCGCGTCTCGGAAATTCGACGACTCGCCGCATTCCGGCGAGCTGATTCTAGATTCCGGCGGGGCAAAGACCGGCGATCTCACCTACACGCCGTTCCGCCAGAATCCCTCTGTATCTAACCATGCTTATAAGGAATACTATTACTTATCAATAAGGAAAATCCTCGTCGGTAACCAGGACGTGAAGGTGCCGTACAAGTATCTAGTGCCGGGATCCGACGGCAGCGGTGGATCTATCATTGATTCCGGCTCTACCTTCACGTTTATGGACAAACCAGTGTTCGAAGCCGTGGCGGAAGCGTTCGAGAAGCAGTTAGCGAATCGGACGAGAGCCACCGACGTGGAATCTGCCACCGGATTACGGCCATGTTTTGACATTTCGAAGGAGAAGTCGGTGGAGTTTCCGGAGTTGATATTTCAGTTTAAAGGCGGAGCGAAATGGGCACTGCCGTTGAATAACTACTTCGCTTTGGTTAGTAGCTCCGGCGTTGCGTGTTTGACGGTTGTAACGCATAAGGAAGCGGCGGGCGGCGGCAGTGGGCCGTCCGTGATTTTGGGGGCTTTTCAACAGCAAAATTTCTATGTGGAATACGATTTGGTGAATGAAAGATTAGGATTTCGGCAACAGAGTTGCAGTTAG

mRNA sequence

ATGGCGGCTCCTCCCCCGCTCTGTTTCTCATACATTCTCCTCCTCTTCTCCGTTTCCGCCATTGTCGACGCCAACGCCAACTCCATCACTCTCCCTCTCTCCGCCTTACCCCACCCTTCTTCCTCAGATCCACTCCAAAATCTCAATTTCCTCGCCTCTGCTTCCCAGAACAGAGCCCATCAAATCAAAACCCCAAAATCCAACTCCGTTTCCAAATCCCCTCTCTCCCCCCATAGCTATGGAGCTTACTCCGCTCCACTCAGCTTCGGTACCCCACCCCAGACTCTCCATTTGATATTTGATACAGGTAGCAGCCTCGTTTGGTTTCCTTGTACTTCCAAATATCTCTGTTCCCAATGTTCGTTTCCCAAAATAGATCCTCTCCGGATCCCAAGATTTGTCCCCAAATTGTCCTCTTCCTCCAAGCTTGTCGGCTGCCAGAATCCAAAATGTGCTTGGGTTTTCGGCCCTGACGTGAAGTCTCAGTGCCGGAATTGTAACCCGAAAACAGAGAACTGTACCCAAACATGTCCTGCTTATGCTGTTCAGTATGGCTCTGGTTCCACGGCCGGGCTTTTGCTATCGGAGACGCTGGATTTTCCCGATCAAAAAATCCCCAATTTCGTTGTGGGGTGTTCGTTTCTTTCGATTCATCAGCCTTCTGGAATCGCTGGATTCGGCCGAGGATCCGAATCGCTTCCGTCGCAAATGGGTCTGAAGAAATTTGCCTACTGTCTCGCGTCTCGGAAATTCGACGACTCGCCGCATTCCGGCGAGCTGATTCTAGATTCCGGCGGGGCAAAGACCGGCGATCTCACCTACACGCCGTTCCGCCAGAATCCCTCTGTATCTAACCATGCTTATAAGGAATACTATTACTTATCAATAAGGAAAATCCTCGTCGGTAACCAGGACGTGAAGGTGCCGTACAAGTATCTAGTGCCGGGATCCGACGGCAGCGGTGGATCTATCATTGATTCCGGCTCTACCTTCACGTTTATGGACAAACCAGTGTTCGAAGCCGTGGCGGAAGCGTTCGAGAAGCAGTTAGCGAATCGGACGAGAGCCACCGACGTGGAATCTGCCACCGGATTACGGCCATGTTTTGACATTTCGAAGGAGAAGTCGGTGGAGTTTCCGGAGTTGATATTTCAGTTTAAAGGCGGAGCGAAATGGGCACTGCCGTTGAATAACTACTTCGCTTTGGTTAGTAGCTCCGGCGTTGCGTGTTTGACGGTTGTAACGCATAAGGAAGCGGCGGGCGGCGGCAGTGGGCCGTCCGTGATTTTGGGGGCTTTTCAACAGCAAAATTTCTATGTGGAATACGATTTGGTGAATGAAAGATTAGGATTTCGGCAACAGAGTTGCAGTTAG

Coding sequence (CDS)

ATGGCGGCTCCTCCCCCGCTCTGTTTCTCATACATTCTCCTCCTCTTCTCCGTTTCCGCCATTGTCGACGCCAACGCCAACTCCATCACTCTCCCTCTCTCCGCCTTACCCCACCCTTCTTCCTCAGATCCACTCCAAAATCTCAATTTCCTCGCCTCTGCTTCCCAGAACAGAGCCCATCAAATCAAAACCCCAAAATCCAACTCCGTTTCCAAATCCCCTCTCTCCCCCCATAGCTATGGAGCTTACTCCGCTCCACTCAGCTTCGGTACCCCACCCCAGACTCTCCATTTGATATTTGATACAGGTAGCAGCCTCGTTTGGTTTCCTTGTACTTCCAAATATCTCTGTTCCCAATGTTCGTTTCCCAAAATAGATCCTCTCCGGATCCCAAGATTTGTCCCCAAATTGTCCTCTTCCTCCAAGCTTGTCGGCTGCCAGAATCCAAAATGTGCTTGGGTTTTCGGCCCTGACGTGAAGTCTCAGTGCCGGAATTGTAACCCGAAAACAGAGAACTGTACCCAAACATGTCCTGCTTATGCTGTTCAGTATGGCTCTGGTTCCACGGCCGGGCTTTTGCTATCGGAGACGCTGGATTTTCCCGATCAAAAAATCCCCAATTTCGTTGTGGGGTGTTCGTTTCTTTCGATTCATCAGCCTTCTGGAATCGCTGGATTCGGCCGAGGATCCGAATCGCTTCCGTCGCAAATGGGTCTGAAGAAATTTGCCTACTGTCTCGCGTCTCGGAAATTCGACGACTCGCCGCATTCCGGCGAGCTGATTCTAGATTCCGGCGGGGCAAAGACCGGCGATCTCACCTACACGCCGTTCCGCCAGAATCCCTCTGTATCTAACCATGCTTATAAGGAATACTATTACTTATCAATAAGGAAAATCCTCGTCGGTAACCAGGACGTGAAGGTGCCGTACAAGTATCTAGTGCCGGGATCCGACGGCAGCGGTGGATCTATCATTGATTCCGGCTCTACCTTCACGTTTATGGACAAACCAGTGTTCGAAGCCGTGGCGGAAGCGTTCGAGAAGCAGTTAGCGAATCGGACGAGAGCCACCGACGTGGAATCTGCCACCGGATTACGGCCATGTTTTGACATTTCGAAGGAGAAGTCGGTGGAGTTTCCGGAGTTGATATTTCAGTTTAAAGGCGGAGCGAAATGGGCACTGCCGTTGAATAACTACTTCGCTTTGGTTAGTAGCTCCGGCGTTGCGTGTTTGACGGTTGTAACGCATAAGGAAGCGGCGGGCGGCGGCAGTGGGCCGTCCGTGATTTTGGGGGCTTTTCAACAGCAAAATTTCTATGTGGAATACGATTTGGTGAATGAAAGATTAGGATTTCGGCAACAGAGTTGCAGTTAG
BLAST of CmoCh06G005120 vs. Swiss-Prot
Match: ASP63_ARATH (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.3e-48
Identity = 146/489 (29.86%), Postives = 220/489 (44.99%), Query Frame = 1

Query: 16  FSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNSVSKSPL 75
           FSVS++       ++  LS   H  SS PL  L   +S S  R  +    +       P+
Sbjct: 20  FSVSSLSTPLLLHLSHSLSTSKH--SSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSLPI 79

Query: 76  SPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVP 135
           S  S   Y   LS G+    + L  DTGS LVWFPC   + C  C    + P        
Sbjct: 80  SSGS--DYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESKPLPPSP----PS 139

Query: 136 KLSSSSKLVGCQNPKCAWVFGPDVKSQC---RNCNP---KTENCTQT---CPAYAVQYGS 195
            LSSS+  V C +P C+        S      NC     +T +C  +   CP +   YG 
Sbjct: 140 SLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGD 199

Query: 196 GSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGL------K 255
           GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  SLP+Q+ +       
Sbjct: 200 GSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGN 259

Query: 256 KFAYCLASRKFDDS--PHSGELIL--------------------DSGGAKTGDLTYTPFR 315
            F+YCL S  FD         LIL                    D    K  +  +T   
Sbjct: 260 SFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEML 319

Query: 316 QNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPV 375
           +NP    H Y  +Y +S++ I +G +++  P        +G GG ++DSG+TFT +    
Sbjct: 320 ENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKF 379

Query: 376 FEAVAEAFEKQLAN-RTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGG-AKWALPL 435
           + +V E F+ ++     RA  VE ++G+ PC+ ++  ++V+ P L+  F G  +   LP 
Sbjct: 380 YNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALVLHFAGNRSSVTLPR 439

Query: 436 NNYFALVSSSG--------VACLTVVTHKEAAGGGSGPSVILGAFQQQNFYVEYDLVNER 458
            NYF      G        + CL ++   + +    G   ILG +QQQ F V YDL+N R
Sbjct: 440 RNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRR 492

BLAST of CmoCh06G005120 vs. Swiss-Prot
Match: APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.1e-38
Identity = 122/403 (30.27%), Postives = 180/403 (44.67%), Query Frame = 1

Query: 65  PKSNSVSKSPLSPHSYGA--YSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSF 124
           P+    S S +S  S G+  Y   L  GTP + ++++ DTGS +VW  C     C +C +
Sbjct: 122 PRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP---CRRC-Y 181

Query: 125 PKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAV 184
            + DP+    F P+ S +   + C +P C        +     CN + + C      Y V
Sbjct: 182 SQSDPI----FDPRKSKTYATIPCSSPHCR-------RLDSAGCNTRRKTC-----LYQV 241

Query: 185 QYGSGS-TAGLLLSETLDFPDQKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMG 244
            YG GS T G   +ETL F   ++    +GC   +       +G+ G G+G  S P Q G
Sbjct: 242 SYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTG 301

Query: 245 LK---KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLS 304
            +   KF+YCL  R     P S   ++    A +    +TP   NP +       +YY+ 
Sbjct: 302 HRFNQKFSYCLVDRSASSKPSS---VVFGNAAVSRIARFTPLLSNPKLDT-----FYYVG 361

Query: 305 IRKILVGNQDVK-VPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRT 364
           +  I VG   V  V          G+GG IIDSG++ T + +P + A+ +AF        
Sbjct: 362 LLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLK 421

Query: 365 RATDVESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVV 424
           RA D         CFD+S    V+ P ++  F+ GA  +LP  NY   V ++G  C    
Sbjct: 422 RAPDFSL---FDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCF--- 481

Query: 425 THKEAAGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
               A  G  G   I+G  QQQ F V YDL + R+GF    C+
Sbjct: 482 ----AFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of CmoCh06G005120 vs. Swiss-Prot
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.0e-37
Identity = 127/386 (32.90%), Postives = 177/386 (45.85%), Query Frame = 1

Query: 81  GAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSS 140
           G Y   ++ GTP  +   I DTGS L+W  C     C+QC F +  P+    F P+ SSS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQC-FSQPTPI----FNPQDSSS 153

Query: 141 SKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGSTA-GLLLSETLD 200
              + C++  C      D+ S         E C      Y   YG GST  G + +ET  
Sbjct: 154 FSTLPCESQYCQ-----DLPS---------ETCNNNECQYTYGYGDGSTTQGYMATETFT 213

Query: 201 FPDQKIPNFVVGCSF----LSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSP 260
           F    +PN   GC            +G+ G G G  SLPSQ+G+ +F+YC+ S     SP
Sbjct: 214 FETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYG-SSSP 273

Query: 261 HSGELILDSGGAKTGDLTYTPFRQ--NPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYL 320
            +  L   + G   G  + T      NP+        YYY++++ I VG  ++ +P    
Sbjct: 274 STLALGSAASGVPEGSPSTTLIHSSLNPT--------YYYITLQGITVGGDNLGIPSSTF 333

Query: 321 VPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESATGLRPCFDISK 380
               DG+GG IIDSG+T T++ +  + AVA+AF  Q+      T  ES++GL  CF    
Sbjct: 334 QLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI---NLPTVDESSSGLSTCFQQPS 393

Query: 381 EKS-VEFPELIFQFKGGAKWALPLNNYFALVS-SSGVACLTVVTHKEAAGGGSGPSV-IL 440
           + S V+ PE+  QF GG    L L     L+S + GV CL       A G  S   + I 
Sbjct: 394 DGSTVQVPEISMQFDGG---VLNLGEQNILISPAEGVICL-------AMGSSSQLGISIF 435

Query: 441 GAFQQQNFYVEYDLVNERLGFRQQSC 457
           G  QQQ   V YDL N  + F    C
Sbjct: 454 GNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of CmoCh06G005120 vs. Swiss-Prot
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.5e-34
Identity = 125/431 (29.00%), Postives = 184/431 (42.69%), Query Frame = 1

Query: 36  LPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNSVSKSPLSPHSY---GAYSAPLSFGTP 95
           L H  S   L     L  A +  + +++  ++     S +    Y   G Y   LS GTP
Sbjct: 45  LEHVDSGKNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTP 104

Query: 96  PQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCA 155
            Q    I DTGS L+W  C     C+QC F +  P+    F P+ SSS   + C +  C 
Sbjct: 105 AQPFSAIMDTGSDLIWTQCQP---CTQC-FNQSTPI----FNPQGSSSFSTLPCSSQLCQ 164

Query: 156 WVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGS-TAGLLLSETLDFPDQKIPNFVVG 215
            +  P               C+     Y   YG GS T G + +ETL F    IPN   G
Sbjct: 165 ALSSP--------------TCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFG 224

Query: 216 CSF----LSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGELILDSGGA 275
           C            +G+ G GRG  SLPSQ+ + KF+YC+       S  S  L+     +
Sbjct: 225 CGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMT--PIGSSTPSNLLLGSLANS 284

Query: 276 KTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGNQDVKV-PYKYLVPGSDGSGGSIID 335
            T     T   Q+  +       +YY+++  + VG+  + + P  + +  ++G+GG IID
Sbjct: 285 VTAGSPNTTLIQSSQIPT-----FYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIID 344

Query: 336 SGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESATGLRPCFDISKEKS-VEFPELIFQ 395
           SG+T T+     +++V + F  Q+          S++G   CF    + S ++ P  +  
Sbjct: 345 SGTTLTYFVNNAYQSVRQEFISQI---NLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMH 404

Query: 396 FKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAAGGGSGPSVILGAFQQQNFYVEYDLV 455
           F GG    LP  NYF +  S+G+ CL       A G  S    I G  QQQN  V YD  
Sbjct: 405 FDGG-DLELPSENYF-ISPSNGLICL-------AMGSSSQGMSIFGNIQQQNMLVVYDTG 434

Query: 456 NERLGFRQQSC 457
           N  + F    C
Sbjct: 465 NSVVSFASAQC 434

BLAST of CmoCh06G005120 vs. Swiss-Prot
Match: AP25_ORYSJ (Aspartyl protease 25 OS=Oryza sativa subsp. japonica GN=AP25 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.0e-34
Identity = 129/456 (28.29%), Postives = 202/456 (44.30%), Query Frame = 1

Query: 13  LLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNS-VS 72
           LLL  ++A V A A  +++  +   HPSS  PL+++  LA     R   + +  + + VS
Sbjct: 9   LLLLLLAATVAAAAAELSVYHNV--HPSSPSPLESIIALARDDDARLLFLSSKAATAGVS 68

Query: 73  KSPL-SPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRI 132
            +P+ S  +  +Y      G+P Q L L  DT +   W  C+    C   S         
Sbjct: 69  SAPVASGQAPPSYVVRAGLGSPSQQLLLALDTSADATWAHCSPCGTCPSSSL-------- 128

Query: 133 PRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGSTA 192
             F P  SSS   + C +  C    G    +     +      T    A++  +   S  
Sbjct: 129 --FAPANSSSYASLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADASFQ 188

Query: 193 GLLLSETLDFPDQKIPNFVVGCSFLSIHQPS------GIAGFGRGSESLPSQMGLKK--- 252
             L S+TL      IPN+  GC   S+  P+      G+ G GRG  +L SQ G      
Sbjct: 189 AALASDTLRLGKDAIPNYTFGC-VSSVTGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGV 248

Query: 253 FAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILV 312
           F+YCL S  +     SG L L +GG +   + YTP  +NP  S+      YY+++  + V
Sbjct: 249 FSYCLPS--YRSYYFSGSLRLGAGGGQPRSVRYTPMLRNPHRSS-----LYYVNVTGLSV 308

Query: 313 GNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVES 372
           G+  VKVP       +    G+++DSG+  T    PV+ A+ E F +Q+A  +  T   S
Sbjct: 309 GHAWVKVPAGSFAFDAATGAGTVVDSGTVITRWTAPVYAALREEFRRQVAAPSGYT---S 368

Query: 373 ATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAAG 432
                 CF+  +  +   P +     GG   ALP+ N     S++ +ACL +    EA  
Sbjct: 369 LGAFDTCFNTDEVAAGGAPAVTVHMDGGVDLALPMENTLIHSSATPLACLAMA---EAPQ 428

Query: 433 GGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
             +    ++   QQQN  V +D+ N R+GF ++SC+
Sbjct: 429 NVNSVVNVIANLQQQNIRVVFDVANSRVGFAKESCN 438

BLAST of CmoCh06G005120 vs. TrEMBL
Match: A0A0A0LBI9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G778440 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 5.9e-230
Identity = 395/459 (86.06%), Postives = 422/459 (91.94%), Query Frame = 1

Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAH 60
           MA+P PL F Y+LL  S+SAI  A++N ITLPL++ PH SS DPLQ L FLAS+SQ RAH
Sbjct: 1   MASPSPLSFFYLLLFSSLSAI--AHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSV KSPLSPHSYGAYS PLSFGTP QTLHLIFDTGSSLVWFPCTS+YLCS+C
Sbjct: 61  QIKTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDP  IPRFVPKLSSSSKLVGCQNPKC+W+FGPDVKSQCR+CNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCSWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFPD+KIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSG+LILDS G K+  LTYTPFRQNPSVSN+AYKEYYYL+IRKI+
Sbjct: 241 KFAYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKII 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQ VKVPYK+LVPG DG+GGSIIDSGSTFTFMDKPV E VA  FEKQLAN TRATDVE
Sbjct: 301 VGNQAVKVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTH--KE 420
           + TGLRPCFDISKEKSV+FPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTH  ++
Sbjct: 361 TLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED 420

Query: 421 AAGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
             GGG GPSVILGAFQQQNFYVEYDLVN+RLGFRQQ+CS
Sbjct: 421 GGGGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 457

BLAST of CmoCh06G005120 vs. TrEMBL
Match: M5VQG8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005104mg PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 5.6e-164
Identity = 279/455 (61.32%), Postives = 353/455 (77.58%), Query Frame = 1

Query: 26  ANSITLPLSALPHPSSSDPLQNLNFLASASQNRAHQIKTPK--SNSVSKSPLSPHSYGAY 85
           ++ ITLPLS  P+  SSDPLQ L+F ASAS +RAH IK  +  ++S+++ PL PHSYG Y
Sbjct: 22  SSKITLPLSPFPNHPSSDPLQALSFHASASISRAHHIKNSRKPNSSLTQVPLFPHSYGDY 81

Query: 86  SAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKL 145
           S  L+FGTPPQT   I DTGSSLVWFPCT +Y+CS+C FP I+P +IP F PKLSSSSK+
Sbjct: 82  SVSLNFGTPPQTSSFIMDTGSSLVWFPCTKRYICSRCQFPNINPAKIPTFKPKLSSSSKI 141

Query: 146 VGCQNPKCAWVFGPDVKSQCRNCN-PKTENCTQTCPAYAVQYGSGSTAGLLLSETLDFPD 205
           VGCQNPKC W+FGP+VKS+C NCN P  +NC+Q CP Y +QYGSG+TAG+LLSETLDFP 
Sbjct: 142 VGCQNPKCGWIFGPEVKSKCPNCNNPSHQNCSQACPTYIIQYGSGTTAGILLSETLDFPK 201

Query: 206 QKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGELIL 265
           + +P+F+VGCSF+SI QP+GIAGFGRG +SLP+QMGL KF+YCL S +FDD+P S +L+L
Sbjct: 202 KIVPDFLVGCSFVSIRQPAGIAGFGRGPQSLPAQMGLTKFSYCLVSHRFDDTPQSSDLVL 261

Query: 266 DSGGA--------------------KTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVG 325
            S  +                    K   L+ TPF++NP   N A++EYYY+ +RK++VG
Sbjct: 262 YSSSSGSSSSSEEEPTIAESQRNKTKLQSLSSTPFQKNPGPPNSAFREYYYIMLRKVIVG 321

Query: 326 NQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESA 385
           N++VK+PYK+LVPG+D SGG+I+DSGSTFTFM+KPVFE VA+ FE Q+AN TRA D+E+ 
Sbjct: 322 NKNVKIPYKFLVPGADSSGGTIVDSGSTFTFMEKPVFEPVAKEFEAQMANYTRAKDLENK 381

Query: 386 TGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAA-G 445
           TGLRPCFDISKEK V+FPEL+FQFKGGAK  LP  NYF++VSSSGV CLT+VT      G
Sbjct: 382 TGLRPCFDISKEKKVDFPELVFQFKGGAKMELPSKNYFSMVSSSGVVCLTIVTDGVVGPG 441

Query: 446 GGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSC 457
           G  GP++ILG +QQQ+F+VEYDL + + GFR+QSC
Sbjct: 442 GNGGPAIILGNYQQQDFHVEYDLQHGKFGFRKQSC 476

BLAST of CmoCh06G005120 vs. TrEMBL
Match: A0A0A0KHK2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G454470 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 1.0e-160
Identity = 275/462 (59.52%), Postives = 351/462 (75.97%), Query Frame = 1

Query: 4   PPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAHQIK 63
           P P  FS  LLL + S+     +++  LPL+  P  S +DP + +N L SAS NRA  +K
Sbjct: 5   PIPFLFSIFLLLPTSSS-----SSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLK 64

Query: 64  TPKSNS---VSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 123
           TP+S S   +    L P SYGAYS  L+FGTPPQ L  IFDTGSSLVWFPCT+ Y CS+C
Sbjct: 65  TPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRC 124

Query: 124 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 183
           SFP +DP  I +FVPKLSSS K+VGC+NPKCAW+FGP++KS+CRNCN K+  C+ +CP Y
Sbjct: 125 SFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGY 184

Query: 184 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 243
            +QYGSG+TAG+LLSETLD  ++++P+F+VGCS +S+HQP+GIAGFGRG ESLPSQM LK
Sbjct: 185 GLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLK 244

Query: 244 KFAYCLASRKFDDSPHSGELILDSGG----AKTGDLTYTPFRQNPSVSNHAYKEYYYLSI 303
           +F++CL SR FDDSP S  L+LDSG     +KT    Y PFR+NPSVSN A++EYYYLS+
Sbjct: 245 RFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSL 304

Query: 304 RKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRA 363
           R+IL+G + VK PYKYLVP S G+GG+IIDSGSTFTF+DKP+FEA+A+  EKQL    RA
Sbjct: 305 RRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRA 364

Query: 364 TDVESATGLRPCFDISK-EKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVT 423
            DVE+ +GLRPCF+I K E+S EFP+++ +FKGG K +L   NY A+V+  GV CLT++T
Sbjct: 365 KDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMT 424

Query: 424 HKEAAGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
            +   GGG GP++ILGAFQQQN  VEYDL  +R+GFR+Q C+
Sbjct: 425 DEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

BLAST of CmoCh06G005120 vs. TrEMBL
Match: B9T7L5_RICCO (Pepsin A, putative OS=Ricinus communis GN=RCOM_0308790 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 8.7e-157
Identity = 280/459 (61.00%), Postives = 351/459 (76.47%), Query Frame = 1

Query: 12  ILLLFSVSAIVDANANSITLPLS-ALPHPSSSDPLQNLNFLASASQNRAHQIKTPKSN-S 71
           + LLFS       + ++IT+PLS  +    SSDP + LN LA+ S +RAH +K+PK+N S
Sbjct: 11  LFLLFSSFVFPFISPSTITIPLSPTITKRPSSDPWEYLNHLATTSISRAHHLKSPKTNFS 70

Query: 72  VSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLR 131
           + K+PL   SYG YS  LS GTP QT+ LI DTGSSLVWFPCTS+Y+C+ C+FP  D  +
Sbjct: 71  LIKTPLFSRSYGGYSMSLSLGTPSQTVKLIMDTGSSLVWFPCTSRYVCASCNFPNTDITK 130

Query: 132 IPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGST 191
           IP+F+P+LSSSSKL+GC+NPKCAWVFG  V+S+C NCNP+ +NCTQ CP Y +QYG GST
Sbjct: 131 IPKFMPRLSSSSKLIGCKNPKCAWVFGSSVQSKCHNCNPQAQNCTQACPPYIIQYGLGST 190

Query: 192 AGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASR 251
           AGLLLSET++FP++ I +F+ GCS LS  QP GIAGFGR  ESLP Q+GLKKF+YCL SR
Sbjct: 191 AGLLLSETINFPNKTISDFLAGCSLLSTRQPEGIAGFGRSQESLPLQLGLKKFSYCLVSR 250

Query: 252 KFDDSPHSGELILDSG----GAKTGDLTYTPFRQN-PSVSNHAYKEYYYLSIRKILVGNQ 311
           +FDDSP S +LILD G     +KT  L+YTPF++N  S SN A++EYYY+ +RKI+VG  
Sbjct: 251 RFDDSPVSSDLILDMGPSTSDSKTTGLSYTPFQKNLASQSNPAFQEYYYVMLRKIIVGKT 310

Query: 312 DVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESATG 371
            VKVPY +LVPGSDG+GG+I+DSGSTFTF++  VFE +A+ FEKQ+AN T AT+V+  TG
Sbjct: 311 HVKVPYSFLVPGSDGNGGTIVDSGSTFTFVEGHVFELLAKEFEKQMANYTVATNVQKLTG 370

Query: 372 LRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAAGGG- 431
           LRPCFDIS EKSV  P+L FQFKGGAK  LPL+NYFA V   GV CLT+V+   AA GG 
Sbjct: 371 LRPCFDISGEKSVVIPDLTFQFKGGAKMQLPLSNYFAFV-DMGVVCLTIVSDNAAALGGD 430

Query: 432 -----SGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
                SGP++ILG FQQQNFY+EYDL N+R GF++QSC+
Sbjct: 431 GGVRSSGPAIILGNFQQQNFYIEYDLENDRFGFKEQSCA 468

BLAST of CmoCh06G005120 vs. TrEMBL
Match: V4SWB8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011613mg PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 2.8e-155
Identity = 279/473 (58.99%), Postives = 352/473 (74.42%), Query Frame = 1

Query: 9   FSYILLLFSVSAIVDANANSITLPLSALP-----HPSSSDPLQNLNFLASASQNRAHQIK 68
           FS ++LLF+  A   ++A ++T+PL+ L      H S SDPL+ L+ LAS+S +RA  +K
Sbjct: 12  FSLLILLFTTDAGAGSSAATVTVPLTPLSTKHYLHHSDSDPLKILHSLASSSLSRARHLK 71

Query: 69  T---PK----------SNSVSKSPLSPHSYGAYSAPLSFGTPPQ-TLHLIFDTGSSLVWF 128
           T   PK          SNS+ K+PLS HSYG YS  LSFGTPPQ +   IFDTGSSLVWF
Sbjct: 72  TKTKPKTKDSNIGSNYSNSLIKTPLSVHSYGGYSISLSFGTPPQASTPFIFDTGSSLVWF 131

Query: 129 PCTSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPK 188
           PCTS+Y C+ C+FP +DP RIP F+PK SSSS+L+GCQNPKC+W+FGP+V+S+C+ CNP+
Sbjct: 132 PCTSRYRCADCNFPNVDPSRIPAFIPKRSSSSQLIGCQNPKCSWIFGPNVESRCKGCNPR 191

Query: 189 TENCTQTCPAYAVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRG 248
            + C   CP Y +QYG G TAGLLLSETL FP + +PNF+VGCS LS  QP+GIAGFGR 
Sbjct: 192 NKTCPLACPPYLIQYGLGFTAGLLLSETLGFPSKTVPNFLVGCSILSNRQPAGIAGFGRS 251

Query: 249 SESLPSQMGLKKFAYCLASRKFDDSPHSGELILD----SGGAKTGDLTYTPFRQNPSVSN 308
           SESLPSQ+GLKKF+YCL SRKFDD+P S  L+LD    SG +KT  L+YTPF +NP  S+
Sbjct: 252 SESLPSQLGLKKFSYCLLSRKFDDAPVSSNLVLDTGSGSGDSKTPGLSYTPFYKNPVGSS 311

Query: 309 HAYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEA 368
            A+ EYYY+ +R+I+VG++ VK+PY YLVPGSDG+GG I+DSGST TFM+ P+FEAVA+ 
Sbjct: 312 SAFGEYYYVGLRQIIVGSKHVKIPYSYLVPGSDGNGGVIVDSGSTLTFMEGPLFEAVAKE 371

Query: 369 FEKQLANRTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSS 428
           F +Q+ N +RA DVE  +GLRPCFDIS +KSV  PELI +FKGGAK ALPL NYFALV +
Sbjct: 372 FIRQMGNYSRAADVEKKSGLRPCFDISGKKSVYLPELILKFKGGAKMALPLENYFALVGN 431

Query: 429 SGVACLTVVTHKEAA-GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
             V CL + T   A    G GP++ILG FQ QNFY+E+DL N+R GF +Q C+
Sbjct: 432 E-VLCLILFTDNAAGPAPGGGPAIILGDFQLQNFYLEFDLANDRFGFAKQKCA 483

BLAST of CmoCh06G005120 vs. TAIR10
Match: AT3G52500.1 (AT3G52500.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 526.9 bits (1356), Expect = 1.2e-149
Identity = 265/472 (56.14%), Postives = 336/472 (71.19%), Query Frame = 1

Query: 9   FSYILLLFSVSAIVDANANSITLPLSALPHPSSS--DPLQNLNFLASASQNRAHQIK--- 68
           F + L+  SV        +++ LPLS   H   S  DP  +L  LA +S  RAH++K   
Sbjct: 6   FFFFLIFLSV-------VSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGT 65

Query: 69  ------------TPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPC 128
                       T  S +V KSPLS  SYG YS  LSFGTP QT+  +FDTGSSLVW PC
Sbjct: 66  SIKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPC 125

Query: 129 TSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTE 188
           TS+YLCS C F  +DP  IPRF+PK SSSSK++GCQ+PKC +++GP+V  QCR C+P T 
Sbjct: 126 TSRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTR 185

Query: 189 NCTQTCPAYAVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSE 248
           NCT  CP Y +QYG GSTAG+L++E LDFPD  +P+FVVGCS +S  QP+GIAGFGRG  
Sbjct: 186 NCTVGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPV 245

Query: 249 SLPSQMGLKKFAYCLASRKFDDSPHSGELILDSG-----GAKTGDLTYTPFRQNPSVSNH 308
           SLPSQM LK+F++CL SR+FDD+  + +L LD+G     G+KT  LTYTPFR+NP+VSN 
Sbjct: 246 SLPSQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNK 305

Query: 309 AYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAF 368
           A+ EYYYL++R+I VG + VK+PYKYL PG++G GGSI+DSGSTFTFM++PVFE VAE F
Sbjct: 306 AFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEF 365

Query: 369 EKQLANRTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSS 428
             Q++N TR  D+E  TGL PCF+IS +  V  PELIF+FKGGAK  LPL+NYF  V ++
Sbjct: 366 ASQMSNYTREKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNT 425

Query: 429 GVACLTVVTHKEA-AGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
              CLTVV+ K     GG+GP++ILG+FQQQN+ VEYDL N+R GF ++ CS
Sbjct: 426 DTVCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

BLAST of CmoCh06G005120 vs. TAIR10
Match: AT5G45120.1 (AT5G45120.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 193.7 bits (491), Expect = 2.4e-49
Identity = 148/486 (30.45%), Postives = 221/486 (45.47%), Query Frame = 1

Query: 10  SYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNS 69
           +++L LF +  ++    N         P  SSS      +FL       +  + TPKS +
Sbjct: 5   THVLFLFLLITLLLNTTNKTQARQHKNPSSSSS------SFLVLTLTKSSVSLPTPKSQT 64

Query: 70  VS--KSPLSPHSY---------GAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTS-KYLC 129
               K PLS               Y   L+ GTPPQ + +  DTGS L W PC +  + C
Sbjct: 65  QERIKKPLSSVDVVMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDC 124

Query: 130 SQCSFPKIDPLRIPR-FVPKLSSSSKLVGCQNPKCAWV------FGPDVKSQCRNCNPKT 189
            +C   K + L+ P  F P  SS+S    C +  C  +      F P   + C       
Sbjct: 125 IECYDLKNNDLKSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLK 184

Query: 190 ENCTQTCPAYAVQYGSGST-AGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRG 249
             C + CP++A  YG G   +G+L  + L    + +P F  GC   +  +P GIAGFGRG
Sbjct: 185 STCVRPCPSFAYTYGEGGLISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFGRG 244

Query: 250 SESLPSQMGL--KKFAYCLASRKFDDSPH-SGELILDSGGAK---TGDLTYTPFRQNPSV 309
             SLPSQ+G   K F++C    KF ++P+ S  LIL +       T  L +TP    P  
Sbjct: 245 LLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQFTPMLNTP-- 304

Query: 310 SNHAYKEYYYLSIRKILVGNQ--DVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEA 369
               Y   YY+ +  I +G      +VP       S G+GG ++DSG+T+T + +P +  
Sbjct: 305 ---MYPNSYYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQ 364

Query: 370 VAEAFEKQLANRTRATDVESATGLRPCFDI--------SKEKSVE--FPELIFQFKGGAK 429
           +    +  +    RAT+ ES TG   C+ +        S E  V   FP + F F   A 
Sbjct: 365 LLTTLQSTI-TYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNAT 424

Query: 430 WALPL-NNYFALVSSSGVACLTVVTHKEAAGGGSGPSVILGAFQQQNFYVEYDLVNERLG 457
             LP  N+++A+ + S  + +  +  +    G  GP+ + G+FQQQN  V YDL  ER+G
Sbjct: 425 LLLPQGNSFYAMSAPSDGSVVQCLLFQNMEDGDYGPAGVFGSFQQQNVKVVYDLEKERIG 478

BLAST of CmoCh06G005120 vs. TAIR10
Match: AT4G16563.1 (AT4G16563.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 193.7 bits (491), Expect = 2.4e-49
Identity = 146/489 (29.86%), Postives = 220/489 (44.99%), Query Frame = 1

Query: 16  FSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAHQIKTPKSNSVSKSPL 75
           FSVS++       ++  LS   H  SS PL  L   +S S  R  +    +       P+
Sbjct: 20  FSVSSLSTPLLLHLSHSLSTSKH--SSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSLPI 79

Query: 76  SPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVP 135
           S  S   Y   LS G+    + L  DTGS LVWFPC   + C  C    + P        
Sbjct: 80  SSGS--DYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILCESKPLPPSP----PS 139

Query: 136 KLSSSSKLVGCQNPKCAWVFGPDVKSQC---RNCNP---KTENCTQT---CPAYAVQYGS 195
            LSSS+  V C +P C+        S      NC     +T +C  +   CP +   YG 
Sbjct: 140 SLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGD 199

Query: 196 GSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGL------K 255
           GS    L S++L  P   + NF  GC+  ++ +P G+AGFGRG  SLP+Q+ +       
Sbjct: 200 GSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGN 259

Query: 256 KFAYCLASRKFDDS--PHSGELIL--------------------DSGGAKTGDLTYTPFR 315
            F+YCL S  FD         LIL                    D    K  +  +T   
Sbjct: 260 SFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEML 319

Query: 316 QNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPV 375
           +NP    H Y  +Y +S++ I +G +++  P        +G GG ++DSG+TFT +    
Sbjct: 320 ENP---KHPY--FYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKF 379

Query: 376 FEAVAEAFEKQLAN-RTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGG-AKWALPL 435
           + +V E F+ ++     RA  VE ++G+ PC+ ++  ++V+ P L+  F G  +   LP 
Sbjct: 380 YNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN--QTVKVPALVLHFAGNRSSVTLPR 439

Query: 436 NNYFALVSSSG--------VACLTVVTHKEAAGGGSGPSVILGAFQQQNFYVEYDLVNER 458
            NYF      G        + CL ++   + +    G   ILG +QQQ F V YDL+N R
Sbjct: 440 RNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRR 492

BLAST of CmoCh06G005120 vs. TAIR10
Match: AT1G25510.1 (AT1G25510.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 168.3 bits (425), Expect = 1.1e-41
Identity = 118/383 (30.81%), Postives = 174/383 (45.43%), Query Frame = 1

Query: 81  GAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSS 140
           G Y   +  G P + ++++ DTGS + W  CT    C+ C + + +P+    F P  SSS
Sbjct: 146 GEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTP---CADC-YHQTEPI----FEPSSSSS 205

Query: 141 SKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGS-TAGLLLSETLD 200
            + + C  P+C  +      S+CRN          TC  Y V YG GS T G   +ETL 
Sbjct: 206 YEPLSCDTPQCNAL----EVSECRNA---------TC-LYEVSYGDGSYTVGDFATETLT 265

Query: 201 FPDQKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPH 260
                + N  VGC   +       +G+ G G G  +LPSQ+    F+YCL  R  D +  
Sbjct: 266 IGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSAS- 325

Query: 261 SGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGNQDVKVPYKYLVPG 320
                +D G + + D    P      + NH    +YYL +  I VG + +++P       
Sbjct: 326 ----TVDFGTSLSPDAVVAPL-----LRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMD 385

Query: 321 SDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESATGLR---PCFDISK 380
             GSGG IIDSG+  T +   ++ ++ ++F K         D+E A G+     C+++S 
Sbjct: 386 ESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVK------GTLDLEKAAGVAMFDTCYNLSA 445

Query: 381 EKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAAGGGSGPSVILGAF 440
           + +VE P + F F GG   ALP  NY   V S G  CL       A    +    I+G  
Sbjct: 446 KTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCL-------AFAPTASSLAIIGNV 483

Query: 441 QQQNFYVEYDLVNERLGFRQQSC 457
           QQQ   V +DL N  +GF    C
Sbjct: 506 QQQGTRVTFDLANSLIGFSSNKC 483

BLAST of CmoCh06G005120 vs. TAIR10
Match: AT2G42980.1 (AT2G42980.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 166.8 bits (421), Expect = 3.2e-41
Identity = 126/398 (31.66%), Postives = 180/398 (45.23%), Query Frame = 1

Query: 81  GAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSS 140
           G Y   +  GTPP+   LI DTGS L W  C   Y C   +    DP        K S+S
Sbjct: 158 GEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGMFYDP--------KTSAS 217

Query: 141 SKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSGS-TAGLLLSETLD 200
            K + C +P+C+ +  PD   QC + N       Q+CP Y   YG  S T G    ET  
Sbjct: 218 FKNITCNDPRCSLISSPDPPVQCESDN-------QSCP-YFYWYGDRSNTTGDFAVETFT 277

Query: 201 F---------PDQKIPNFVVGCSFLS---IHQPSGIAGFGRGSESLPSQMGL---KKFAY 260
                      + K+ N + GC   +       SG+ G GRG  S  SQ+       F+Y
Sbjct: 278 VNLTTTEGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSY 337

Query: 261 CLASRKFDDSPHSGELIL--DSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVG 320
           CL  R  + +  S +LI   D       +L +T F        ++ + +YY+ I+ ILVG
Sbjct: 338 CLVDRNSNTNV-SSKLIFGEDKDLLNHTNLNFTSFVNG---KENSVETFYYIQIKSILVG 397

Query: 321 NQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAF-EKQLANRTRATDVES 380
            + + +P +     SDG GG+IIDSG+T ++  +P +E +   F EK   N     D   
Sbjct: 398 GKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPV 457

Query: 381 ATGLRPCFDIS--KEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEA 440
              L PCF++S  +E ++  PEL   F  G  W  P  N F  +S   + CL ++     
Sbjct: 458 ---LDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSED-LVCLAIL----- 517

Query: 441 AGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
            G       I+G +QQQNF++ YD    RLGF    C+
Sbjct: 518 -GTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKCA 525

BLAST of CmoCh06G005120 vs. NCBI nr
Match: gi|659084466|ref|XP_008442902.1| (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo])

HSP 1 Score: 813.1 bits (2099), Expect = 2.4e-232
Identity = 397/457 (86.87%), Postives = 423/457 (92.56%), Query Frame = 1

Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAH 60
           MA+P PL F YILL  S+SAI  +N+N ITLPL++ PH SSSDPLQ L FLASAS+NRAH
Sbjct: 1   MASPSPLSFFYILLFSSLSAI--SNSNPITLPLNSSPHLSSSDPLQALTFLASASKNRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           +IKTPKSNSVSKSPLSPHSYGAYS PLSFGTP QTLHLIFDTGSSLVWFPCTS+YLC++C
Sbjct: 61  RIKTPKSNSVSKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCTEC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDP  IPRFVPKLSSSSKLVGCQNPKCAW+FGPDVKSQCR+CNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCAWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFP++KIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPNKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDS HSG+LILDS G KT  LTYT FRQNPSVSNHAYKEYYYL+IRKI+
Sbjct: 241 KFAYCLASRKFDDSAHSGQLILDSSGVKTSGLTYTSFRQNPSVSNHAYKEYYYLNIRKII 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQ VKVPYKYLVPG DG+GGSIIDSGSTFTFMDKPV + VA+ FEKQLANRTRATDVE
Sbjct: 301 VGNQAVKVPYKYLVPGPDGNGGSIIDSGSTFTFMDKPVLDVVAQEFEKQLANRTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAA 420
           + TGLRPCFD+SKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTH    
Sbjct: 361 TLTGLRPCFDVSKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHNTED 420

Query: 421 GGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
           GGG GPSVILGAFQQQNFYVEYDLVNERLGFR+Q+C+
Sbjct: 421 GGGGGPSVILGAFQQQNFYVEYDLVNERLGFRKQTCT 455

BLAST of CmoCh06G005120 vs. NCBI nr
Match: gi|449437856|ref|XP_004136706.1| (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis sativus])

HSP 1 Score: 804.7 bits (2077), Expect = 8.5e-230
Identity = 395/459 (86.06%), Postives = 422/459 (91.94%), Query Frame = 1

Query: 1   MAAPPPLCFSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAH 60
           MA+P PL F Y+LL  S+SAI  A++N ITLPL++ PH SS DPLQ L FLAS+SQ RAH
Sbjct: 1   MASPSPLSFFYLLLFSSLSAI--AHSNPITLPLNSFPHLSSPDPLQALTFLASSSQTRAH 60

Query: 61  QIKTPKSNSVSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQC 120
           QIKTPKSNSV KSPLSPHSYGAYS PLSFGTP QTLHLIFDTGSSLVWFPCTS+YLCS+C
Sbjct: 61  QIKTPKSNSVFKSPLSPHSYGAYSTPLSFGTPQQTLHLIFDTGSSLVWFPCTSRYLCSEC 120

Query: 121 SFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAY 180
           SFPKIDP  IPRFVPKLSSSSKLVGCQNPKC+W+FGPDVKSQCR+CNPKTENCTQTCPAY
Sbjct: 121 SFPKIDPTGIPRFVPKLSSSSKLVGCQNPKCSWIFGPDVKSQCRSCNPKTENCTQTCPAY 180

Query: 181 AVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240
            VQYGSGSTAGLLLSETLDFPD+KIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK
Sbjct: 181 VVQYGSGSTAGLLLSETLDFPDKKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLK 240

Query: 241 KFAYCLASRKFDDSPHSGELILDSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKIL 300
           KFAYCLASRKFDDSPHSG+LILDS G K+  LTYTPFRQNPSVSN+AYKEYYYL+IRKI+
Sbjct: 241 KFAYCLASRKFDDSPHSGQLILDSTGVKSSGLTYTPFRQNPSVSNNAYKEYYYLNIRKII 300

Query: 301 VGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVE 360
           VGNQ VKVPYK+LVPG DG+GGSIIDSGSTFTFMDKPV E VA  FEKQLAN TRATDVE
Sbjct: 301 VGNQAVKVPYKFLVPGPDGNGGSIIDSGSTFTFMDKPVLEVVAREFEKQLANWTRATDVE 360

Query: 361 SATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTH--KE 420
           + TGLRPCFDISKEKSV+FPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTH  ++
Sbjct: 361 TLTGLRPCFDISKEKSVKFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHQMED 420

Query: 421 AAGGGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSCS 458
             GGG GPSVILGAFQQQNFYVEYDLVN+RLGFRQQ+CS
Sbjct: 421 GGGGGGGPSVILGAFQQQNFYVEYDLVNQRLGFRQQTCS 457

BLAST of CmoCh06G005120 vs. NCBI nr
Match: gi|1009161294|ref|XP_015898820.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Ziziphus jujuba])

HSP 1 Score: 601.7 bits (1550), Expect = 1.1e-168
Identity = 289/455 (63.52%), Postives = 362/455 (79.56%), Query Frame = 1

Query: 9   FSYILLLFSVSAIVDANANSITLPLSALPHPSSSDPLQNLNFLASASQNRAHQIKTPKSN 68
           FS  L + S S+       SI++ +S      SSDP Q+LNFLAS S +RAH +K PKSN
Sbjct: 14  FSLFLSVASSSSTSPPKPTSISIQISPFSKHPSSDPFQSLNFLASLSISRAHHLKHPKSN 73

Query: 69  S-VSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDP 128
           S ++K PL P  YG YS  L+FGTPPQ +  + DTGSSLVW PCTS+YLCS+CSFP I P
Sbjct: 74  SSLTKVPLYPRGYGGYSIFLNFGTPPQKISFVMDTGSSLVWLPCTSRYLCSKCSFPNIVP 133

Query: 129 LRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTCPAYAVQYGSG 188
            +IP F+PKLSSSSK+VGC+NPKC WV GPDVK  C++C+P ++NC+Q CPAY +QYGSG
Sbjct: 134 AKIPTFIPKLSSSSKIVGCKNPKCGWVLGPDVK--CQDCDPSSKNCSQPCPAYIIQYGSG 193

Query: 189 STAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLA 248
           +TAGLL+SE+LDFP++ +P+F+VGCSFLS  QPSGIAGFGRG +SLPSQMGL KF+YCL 
Sbjct: 194 TTAGLLISESLDFPEKTVPDFLVGCSFLSFRQPSGIAGFGRGPQSLPSQMGLSKFSYCLI 253

Query: 249 SRKFDDSPHSGELIL----DSGGAKTGDLTYTPFRQNPSVSNHAYKEYYYLSIRKILVGN 308
           S KFDD+  S  L+L    DSG +K  DL+YTPF++NP VSN A+ EYYY+ +RK++VG 
Sbjct: 254 SHKFDDTQESSNLVLYSGSDSGNSKATDLSYTPFQKNPEVSNPAFHEYYYVLLRKVIVGG 313

Query: 309 QDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESAT 368
             VK+PYK+LVPGS+G+GG+I+DSGSTFTFM+KPVFEAV++ F KQ+ N TRATD+E+ T
Sbjct: 314 TRVKIPYKFLVPGSEGNGGTIVDSGSTFTFMEKPVFEAVSQEFAKQMVNYTRATDIENRT 373

Query: 369 GLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAAGGG 428
           GL+PCFDIS+EKSV FPEL+FQFKGGAK ALP+ NYF+LV+ SG+ CLT+VT  E AG  
Sbjct: 374 GLQPCFDISREKSVNFPELVFQFKGGAKMALPVANYFSLVTDSGIICLTIVT-DEVAGPS 433

Query: 429 --SGPSVILGAFQQQNFYVEYDLVNERLGFRQQSC 457
             SGP++ILG +QQQNF++EYDL NER GFR+QSC
Sbjct: 434 FTSGPAIILGNYQQQNFHIEYDLENERFGFRRQSC 465

BLAST of CmoCh06G005120 vs. NCBI nr
Match: gi|470135396|ref|XP_004303503.1| (PREDICTED: aspartic proteinase nepenthesin-2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 599.7 bits (1545), Expect = 4.1e-168
Identity = 293/467 (62.74%), Postives = 367/467 (78.59%), Query Frame = 1

Query: 1   MAAPPPLCFSYILLLFSVSAI-VDANANSITLPLSALP-HPSSSDPLQNLNFLASASQNR 60
           MA P PL    +L L S+S++ + A ++ +TLPLS L  HPSSSDP+Q LN L+SAS +R
Sbjct: 1   MATPNPL----LLFLISLSSLFLLAFSSKLTLPLSPLAKHPSSSDPIQTLNLLSSASLSR 60

Query: 61  AHQIKTPKSNS-VSKSPLSPHSYGAYSAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLC 120
           AH +K PK NS  +K PL P SYG YS  LSFGTPPQ    + DTGSSLVWFPCTS+YLC
Sbjct: 61  AHHLKRPKHNSSATKVPLYPRSYGGYSISLSFGTPPQISTFVMDTGSSLVWFPCTSRYLC 120

Query: 121 SQCSFPKIDPLRIPRFVPKLSSSSKLVGCQNPKCAWVFGPDVKSQCRNCNPKTENCTQTC 180
           S+CSFP IDP  IP F+PKLSSS++L+GC+NPKCAW+FGP+V ++C        N +Q C
Sbjct: 121 SRCSFPNIDPSTIPAFIPKLSSSARLLGCKNPKCAWIFGPEVNTKC-------PNSSQAC 180

Query: 181 PAYAVQYGSGSTAGLLLSETLDFPDQKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQM 240
           P+Y +QYGSG+TAG+LLSE+LDFPD+ +P+F+VGCSFLSI QP+G+AGFGRG +SLP QM
Sbjct: 181 PSYVIQYGSGTTAGVLLSESLDFPDKTVPDFLVGCSFLSIRQPAGMAGFGRGPQSLPVQM 240

Query: 241 GLKKFAYCLASRKFDDSPHSGELILDSGGAKTGD-------LTYTPFRQNPSVSNHAYKE 300
           GL KF+YCL S +FDD+P S +L+L SG    GD       ++YTPF++NP  +N AY+E
Sbjct: 241 GLSKFSYCLVSHRFDDTPVSSDLVLYSGSTSDGDEIDDNHDISYTPFQKNPGAANTAYRE 300

Query: 301 YYYLSIRKILVGNQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQL 360
           YYYL++RK++VG + VK+PYKYLVPG D +GG+I+DSGSTFTFM++PVFEAVAEAF  Q+
Sbjct: 301 YYYLALRKVIVGKKHVKIPYKYLVPGEDDNGGTIVDSGSTFTFMERPVFEAVAEAFATQM 360

Query: 361 ANRTRATDVESATGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVAC 420
              TRA D+E+ TGL+PCFDISKE+ V+FPEL+FQFKGGAK A+PLNNYFALV+S GV C
Sbjct: 361 EKYTRAGDIENRTGLKPCFDISKEEKVDFPELVFQFKGGAKMAMPLNNYFALVTSDGVVC 420

Query: 421 LTVVTHKEAAGG-GSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSC 457
           LT+VT   A  G  +GP+VILG FQQQNFYVEYDL  ER GF++QSC
Sbjct: 421 LTIVTDGVAGPGVAAGPAVILGNFQQQNFYVEYDLERERFGFKKQSC 456

BLAST of CmoCh06G005120 vs. NCBI nr
Match: gi|645276803|ref|XP_008243463.1| (PREDICTED: aspartic proteinase nepenthesin-2-like [Prunus mume])

HSP 1 Score: 595.5 bits (1534), Expect = 7.8e-167
Identity = 287/455 (63.08%), Postives = 357/455 (78.46%), Query Frame = 1

Query: 26  ANSITLPLSALPHPSSSDPLQNLNFLASASQNRAHQIKTPK--SNSVSKSPLSPHSYGAY 85
           ++ +TLPLS  P+  SSDPLQ L+F ASAS +RAH IK  +  ++S+++ PL PHSYG Y
Sbjct: 22  SSKLTLPLSPFPNYPSSDPLQALSFHASASISRAHHIKNSRKPNSSLTQVPLFPHSYGDY 81

Query: 86  SAPLSFGTPPQTLHLIFDTGSSLVWFPCTSKYLCSQCSFPKIDPLRIPRFVPKLSSSSKL 145
           S  L+FGTPPQT   I DTGSSLVWFPCT +Y CS+C FP I+P +IP F PKLSSSSK+
Sbjct: 82  SVSLNFGTPPQTSSFIMDTGSSLVWFPCTKRYSCSRCQFPNINPAKIPTFKPKLSSSSKI 141

Query: 146 VGCQNPKCAWVFGPDVKSQCRNCN-PKTENCTQTCPAYAVQYGSGSTAGLLLSETLDFPD 205
           VGCQNPKC W+FGP+VKS+C NCN P  +NC+QTCP Y +QYGSG+TAG+LLSETL+FP 
Sbjct: 142 VGCQNPKCGWIFGPEVKSKCPNCNNPSPQNCSQTCPTYIIQYGSGTTAGILLSETLNFPK 201

Query: 206 QKIPNFVVGCSFLSIHQPSGIAGFGRGSESLPSQMGLKKFAYCLASRKFDDSPHSGELIL 265
           + +P+F+VGCSFLSI QPSGIAGFGRG +SLP+QMGL KF+YCL S KFDD+P S +L+L
Sbjct: 202 KIVPDFLVGCSFLSIRQPSGIAGFGRGPQSLPAQMGLSKFSYCLVSHKFDDTPQSSDLVL 261

Query: 266 ---DSGGAKTGD-----------------LTYTPFRQNPSVSNHAYKEYYYLSIRKILVG 325
               SG + + +                 L+ TPF++NP   N A++EYYY+ +RK++VG
Sbjct: 262 YSSSSGSSSSSEEEPTIAESQRNKTRLQSLSSTPFQKNPGPPNSAFREYYYIMLRKVIVG 321

Query: 326 NQDVKVPYKYLVPGSDGSGGSIIDSGSTFTFMDKPVFEAVAEAFEKQLANRTRATDVESA 385
           N++VK+PYK+LVPG+D SGG+I+DSGSTFTFM+KPVFE VAE FE Q+AN TRA + E+ 
Sbjct: 322 NKNVKIPYKFLVPGADSSGGTIVDSGSTFTFMEKPVFELVAEEFEAQMANYTRAKEWENK 381

Query: 386 TGLRPCFDISKEKSVEFPELIFQFKGGAKWALPLNNYFALVSSSGVACLTVVTHKEAA-G 445
           TGLRPCFDISKEK V+FPEL+FQFKGGAK  LPL NYF+LVSSSGV CLT+VT   A  G
Sbjct: 382 TGLRPCFDISKEKKVDFPELVFQFKGGAKMELPLTNYFSLVSSSGVVCLTIVTDGVAGPG 441

Query: 446 GGSGPSVILGAFQQQNFYVEYDLVNERLGFRQQSC 457
           G  GP++ILG +QQQNF+VEYDL +ER GFR+QSC
Sbjct: 442 GNGGPAIILGNYQQQNFHVEYDLQHERFGFRKQSC 476

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ASP63_ARATH4.3e-4829.86Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana GN=At4g16563 PE=2 S... [more]
APF2_ARATH3.1e-3830.27Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1[more]
NEP2_NEPGR2.0e-3732.90Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1[more]
NEP1_NEPGR3.5e-3429.00Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1[more]
AP25_ORYSJ6.0e-3428.29Aspartyl protease 25 OS=Oryza sativa subsp. japonica GN=AP25 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LBI9_CUCSA5.9e-23086.06Uncharacterized protein OS=Cucumis sativus GN=Csa_3G778440 PE=3 SV=1[more]
M5VQG8_PRUPE5.6e-16461.32Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005104mg PE=3 SV=1[more]
A0A0A0KHK2_CUCSA1.0e-16059.52Uncharacterized protein OS=Cucumis sativus GN=Csa_6G454470 PE=3 SV=1[more]
B9T7L5_RICCO8.7e-15761.00Pepsin A, putative OS=Ricinus communis GN=RCOM_0308790 PE=3 SV=1[more]
V4SWB8_9ROSI2.8e-15558.99Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011613mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G52500.11.2e-14956.14 Eukaryotic aspartyl protease family protein[more]
AT5G45120.12.4e-4930.45 Eukaryotic aspartyl protease family protein[more]
AT4G16563.12.4e-4929.86 Eukaryotic aspartyl protease family protein[more]
AT1G25510.11.1e-4130.81 Eukaryotic aspartyl protease family protein[more]
AT2G42980.13.2e-4131.66 Eukaryotic aspartyl protease family protein[more]
Match NameE-valueIdentityDescription
gi|659084466|ref|XP_008442902.1|2.4e-23286.87PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo][more]
gi|449437856|ref|XP_004136706.1|8.5e-23086.06PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis sativus][more]
gi|1009161294|ref|XP_015898820.1|1.1e-16863.52PREDICTED: aspartic proteinase nepenthesin-1 [Ziziphus jujuba][more]
gi|470135396|ref|XP_004303503.1|4.1e-16862.74PREDICTED: aspartic proteinase nepenthesin-2 [Fragaria vesca subsp. vesca][more]
gi|645276803|ref|XP_008243463.1|7.8e-16763.08PREDICTED: aspartic proteinase nepenthesin-2-like [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001461Aspartic_peptidase_A1
IPR001969Aspartic_peptidase_AS
IPR021109Peptidase_aspartic_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005618 cell wall
cellular_component GO:0005575 cellular_component
molecular_function GO:0004190 aspartic-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G005120.1CmoCh06G005120.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 323..334
score: 2.5E-7coord: 428..443
score: 2.5E-7coord: 89..109
score: 2.
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 7..457
score: 1.4E
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 98..109
scor
IPR021109Aspartic peptidase domainGENE3DG3DSA:2.40.70.10coord: 268..457
score: 2.6E-53coord: 78..255
score: 4.2
IPR021109Aspartic peptidase domainunknownSSF50630Acid proteasescoord: 77..456
score: 2.17
NoneNo IPR availablePANTHERPTHR13683:SF340ASPARTYL PROTEASE FAMILY PROTEINcoord: 7..457
score: 1.4E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh06G005120CmoCh14G003440Cucurbita moschata (Rifu)cmocmoB222
The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh06G005120Cucumber (Chinese Long) v3cmocucB0951
CmoCh06G005120Cucumber (Chinese Long) v3cmocucB0979
CmoCh06G005120Cucumber (Chinese Long) v3cmocucB0982
CmoCh06G005120Watermelon (97103) v2cmowmbB811
CmoCh06G005120Watermelon (97103) v2cmowmbB827
CmoCh06G005120Wax gourdcmowgoB0974
CmoCh06G005120Wax gourdcmowgoB1025
CmoCh06G005120Wax gourdcmowgoB1032
CmoCh06G005120Cucurbita moschata (Rifu)cmocmoB423
CmoCh06G005120Cucurbita moschata (Rifu)cmocmoB454
CmoCh06G005120Cucurbita moschata (Rifu)cmocmoB483
CmoCh06G005120Cucumber (Gy14) v1cgycmoB0149
CmoCh06G005120Cucumber (Gy14) v1cgycmoB0536
CmoCh06G005120Cucurbita maxima (Rimu)cmacmoB570
CmoCh06G005120Cucurbita maxima (Rimu)cmacmoB665
CmoCh06G005120Cucurbita maxima (Rimu)cmacmoB874
CmoCh06G005120Wild cucumber (PI 183967)cmocpiB810
CmoCh06G005120Wild cucumber (PI 183967)cmocpiB838
CmoCh06G005120Wild cucumber (PI 183967)cmocpiB842
CmoCh06G005120Cucumber (Chinese Long) v2cmocuB828
CmoCh06G005120Cucumber (Chinese Long) v2cmocuB832
CmoCh06G005120Melon (DHL92) v3.5.1cmomeB754
CmoCh06G005120Melon (DHL92) v3.5.1cmomeB763
CmoCh06G005120Watermelon (Charleston Gray)cmowcgB739
CmoCh06G005120Watermelon (97103) v1cmowmB799
CmoCh06G005120Cucurbita pepo (Zucchini)cmocpeB774
CmoCh06G005120Cucurbita pepo (Zucchini)cmocpeB782
CmoCh06G005120Bottle gourd (USVL1VR-Ls)cmolsiB737
CmoCh06G005120Bottle gourd (USVL1VR-Ls)cmolsiB740
CmoCh06G005120Cucumber (Gy14) v2cgybcmoB130
CmoCh06G005120Cucumber (Gy14) v2cgybcmoB833
CmoCh06G005120Cucumber (Gy14) v2cgybcmoB837
CmoCh06G005120Melon (DHL92) v3.6.1cmomedB856
CmoCh06G005120Melon (DHL92) v3.6.1cmomedB867
CmoCh06G005120Silver-seed gourdcarcmoB0337
CmoCh06G005120Silver-seed gourdcarcmoB0562
CmoCh06G005120Silver-seed gourdcarcmoB1099
CmoCh06G005120Silver-seed gourdcarcmoB1239