CmoCh06G000100 (gene) Cucurbita moschata (Rifu)

NameCmoCh06G000100
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionPectinesterase (3.1.1.11)
LocationCmo_Chr06 : 86808 .. 88832 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTACTTTAACCTAAGGCAGAGTTCCCATCTCTGCCTTAGCCATTTCCCGTTATTCCAACTCATCCACCCACCATCCACCATGGTCTTCCAAGATTTCGACCAGATCTCCGAACGCCGTCGCGCCGAGAGAGCGCGCAAGTTCCGTAATAGGGTCATCATCGCGGTCGTCTCAACCTTCCTCATCCTTGTTCTCGTCGTCGCCGCCGTCTTCGTCCAAGTCTCCCCGCCCACCAAATCCACCACCAGAGACGGCGCCGGCAGCGGCAGCAGCCGCAGCAAGGGAAGCTCCAAAGATCATGCGACATCCGACGTCAAACAGGTGTCCCGCGCCCAGAAAATGATTACCATGATATGCAACTCCACAGACTATAAAGCCAAATGCGAGAGCACTTTGAAGGAAGGCGTAGAGACCCACCCCGATTCCTCCGACCCCAAAGACCTCATCAAGCTCGCCATCGCCGCCGTCTCCACCGAGGTTAACTCCGCCCTGAAAAAAGCTTCCGCCATGAACTTCCCCACCCCGGAGGAAAAAGGGGCGTTCGAGGACTGCAAGCTCCTGTTGGAAGAAGCGGTGGAGGAATTAGACAGGTCTGTGAGCGAATTGAGCAGGAAGGATCTGGGAAAGCTGACAGAGAAAACGACGCCGGATCTCAACAACTGGCTGAGCGCGGTGATGTCGTATCACGCCACTTGCGTGGATGGGTTTCCAGGGGGGAAGTCGAAGACCGAAATGGAGGCGACGTTGAAGGCCGGAAAGGAACTGACGAGTAATTCGCTGGCGATGATTTCGCAGGTGGCTACGTTCTTCTCAACGTTTGAAAAGTCGGATGGTGGAGGAGCTGCAACACGACGTCGTCTAATGGCCAAAGATGGGCTACCCAGCTGGATGACCAGCTACGAACGCAGGATGTTGAAGCGCGATGCGGAAGACGATAAGCCGACGCCTAATGTTGTGGTCGCCAAAGATGGGTCCGGAAATTTCAAGACCATTAACGAAGCGTTGGCTGCCATGCCTGCAAAATATGATGGACGGTAGGCTCTGTTTTTTTTTTTTTTTAGTACTTTATGTAATTCATATGAATAATGTATTGTATTAAAATGGATGAAAACAAGCAGGTACGTGATCTATGTTAAGGAAGGGATCTACGAGGAGACGGTGGTGATTACCAAAAAAATGGCGAATGTTACAATGTACGGAGATGGATCTCAGAAGAGCATGATCAGTGGGAATAAAAACTCAGCGGACGGAGTTCGAGCCTTCCAAACCGCATCTTTTGTGGCACTTGGAGACGGGTTGTTGGCGAAAGGGATCGGATTCAGAAACACTGCAGGTCCAGAAAAGCAGCAGGCCGTAGCCGCCAGAGTCCAGGGCGACAGAGCCATATTCGTGAACTGTCGGTTCGAGGGGTTCCAAGACACGTTGTACGCGCAAGCCCATCGGCAGTTCTACAGGAGCTGCTTGATTACAGGCACAGTGGACATCATCTTCGGGGACGCGGCGGCCATTTTCCAAAATTGTAATTTCATGTTGAAAAAGCCGTTGGATAAGCAAGCGAACGTAGTAACGGCACAGGGGAGAAGTGACAGACATGAAACCACGGCCACTGTGCTGCAAAACTGCAAGATATTGCCGGACAAGACGCTGGAGGGTGTGAAGGCGCAGTTCAAAAGCTACTTGGGGAGGCCTCGGAATGAGTACTCCAGGACCATAGTAATGGAGTCCGAAATTGAAGATATCATTCACCCCGATGGGTGGATGCCTTGGGACGGGGACTTTGCACTCAAAACCTTGTACTATGCTGAGTTTAACAACAAAGGGCCTGGGGCTAAGACCGATGCCAGAGTGAAATGGCCTGGTTTTAAGGTGATTGATAAGGAAGAGGCTGCAAAGTTTACGATAGGAACTTTTCTAGACGTGGATTGGATCCAGAGCTCCTCGGTTTCTGTTCATGTCGGCCTGTTTTGATATAACACCAAAAAAGAAAAAAAAAAAGAAAAATCCATTTTCTCATCTCCTTTTCGT

mRNA sequence

CTTTACTTTAACCTAAGGCAGAGTTCCCATCTCTGCCTTAGCCATTTCCCGTTATTCCAACTCATCCACCCACCATCCACCATGGTCTTCCAAGATTTCGACCAGATCTCCGAACGCCGTCGCGCCGAGAGAGCGCGCAAGTTCCGTAATAGGGTCATCATCGCGGTCGTCTCAACCTTCCTCATCCTTGTTCTCGTCGTCGCCGCCGTCTTCGTCCAAGTCTCCCCGCCCACCAAATCCACCACCAGAGACGGCGCCGGCAGCGGCAGCAGCCGCAGCAAGGGAAGCTCCAAAGATCATGCGACATCCGACGTCAAACAGGTGTCCCGCGCCCAGAAAATGATTACCATGATATGCAACTCCACAGACTATAAAGCCAAATGCGAGAGCACTTTGAAGGAAGGCGTAGAGACCCACCCCGATTCCTCCGACCCCAAAGACCTCATCAAGCTCGCCATCGCCGCCGTCTCCACCGAGGTTAACTCCGCCCTGAAAAAAGCTTCCGCCATGAACTTCCCCACCCCGGAGGAAAAAGGGGCGTTCGAGGACTGCAAGCTCCTGTTGGAAGAAGCGGTGGAGGAATTAGACAGGTCTGTGAGCGAATTGAGCAGGAAGGATCTGGGAAAGCTGACAGAGAAAACGACGCCGGATCTCAACAACTGGCTGAGCGCGGTGATGTCGTATCACGCCACTTGCGTGGATGGGTTTCCAGGGGGGAAGTCGAAGACCGAAATGGAGGCGACGTTGAAGGCCGGAAAGGAACTGACGAGTAATTCGCTGGCGATGATTTCGCAGGTGGCTACGTTCTTCTCAACGTTTGAAAAGTCGGATGGTGGAGGAGCTGCAACACGACGTCGTCTAATGGCCAAAGATGGGCTACCCAGCTGGATGACCAGCTACGAACGCAGGATGTTGAAGCGCGATGCGGAAGACGATAAGCCGACGCCTAATGTTGTGGTCGCCAAAGATGGGTCCGGAAATTTCAAGACCATTAACGAAGCGTTGGCTGCCATGCCTGCAAAATATGATGGACGGTACGTGATCTATGTTAAGGAAGGGATCTACGAGGAGACGGTGGTGATTACCAAAAAAATGGCGAATGTTACAATGTACGGAGATGGATCTCAGAAGAGCATGATCAGTGGGAATAAAAACTCAGCGGACGGAGTTCGAGCCTTCCAAACCGCATCTTTTGTGGCACTTGGAGACGGGTTGTTGGCGAAAGGGATCGGATTCAGAAACACTGCAGGTCCAGAAAAGCAGCAGGCCGTAGCCGCCAGAGTCCAGGGCGACAGAGCCATATTCGTGAACTGTCGGTTCGAGGGGTTCCAAGACACGTTGTACGCGCAAGCCCATCGGCAGTTCTACAGGAGCTGCTTGATTACAGGCACAGTGGACATCATCTTCGGGGACGCGGCGGCCATTTTCCAAAATTGTAATTTCATGTTGAAAAAGCCGTTGGATAAGCAAGCGAACGTAGTAACGGCACAGGGGAGAAGTGACAGACATGAAACCACGGCCACTGTGCTGCAAAACTGCAAGATATTGCCGGACAAGACGCTGGAGGGTGTGAAGGCGCAGTTCAAAAGCTACTTGGGGAGGCCTCGGAATGAGTACTCCAGGACCATAGTAATGGAGTCCGAAATTGAAGATATCATTCACCCCGATGGGTGGATGCCTTGGGACGGGGACTTTGCACTCAAAACCTTGTACTATGCTGAGTTTAACAACAAAGGGCCTGGGGCTAAGACCGATGCCAGAGTGAAATGGCCTGGTTTTAAGGTGATTGATAAGGAAGAGGCTGCAAAGTTTACGATAGGAACTTTTCTAGACGTGGATTGGATCCAGAGCTCCTCGGTTTCTGTTCATGTCGGCCTGTTTTGATATAACACCAAAAAAGAAAAAAAAAAAGAAAAATCCATTTTCTCATCTCCTTTTCGT

Coding sequence (CDS)

ATGGTCTTCCAAGATTTCGACCAGATCTCCGAACGCCGTCGCGCCGAGAGAGCGCGCAAGTTCCGTAATAGGGTCATCATCGCGGTCGTCTCAACCTTCCTCATCCTTGTTCTCGTCGTCGCCGCCGTCTTCGTCCAAGTCTCCCCGCCCACCAAATCCACCACCAGAGACGGCGCCGGCAGCGGCAGCAGCCGCAGCAAGGGAAGCTCCAAAGATCATGCGACATCCGACGTCAAACAGGTGTCCCGCGCCCAGAAAATGATTACCATGATATGCAACTCCACAGACTATAAAGCCAAATGCGAGAGCACTTTGAAGGAAGGCGTAGAGACCCACCCCGATTCCTCCGACCCCAAAGACCTCATCAAGCTCGCCATCGCCGCCGTCTCCACCGAGGTTAACTCCGCCCTGAAAAAAGCTTCCGCCATGAACTTCCCCACCCCGGAGGAAAAAGGGGCGTTCGAGGACTGCAAGCTCCTGTTGGAAGAAGCGGTGGAGGAATTAGACAGGTCTGTGAGCGAATTGAGCAGGAAGGATCTGGGAAAGCTGACAGAGAAAACGACGCCGGATCTCAACAACTGGCTGAGCGCGGTGATGTCGTATCACGCCACTTGCGTGGATGGGTTTCCAGGGGGGAAGTCGAAGACCGAAATGGAGGCGACGTTGAAGGCCGGAAAGGAACTGACGAGTAATTCGCTGGCGATGATTTCGCAGGTGGCTACGTTCTTCTCAACGTTTGAAAAGTCGGATGGTGGAGGAGCTGCAACACGACGTCGTCTAATGGCCAAAGATGGGCTACCCAGCTGGATGACCAGCTACGAACGCAGGATGTTGAAGCGCGATGCGGAAGACGATAAGCCGACGCCTAATGTTGTGGTCGCCAAAGATGGGTCCGGAAATTTCAAGACCATTAACGAAGCGTTGGCTGCCATGCCTGCAAAATATGATGGACGGTACGTGATCTATGTTAAGGAAGGGATCTACGAGGAGACGGTGGTGATTACCAAAAAAATGGCGAATGTTACAATGTACGGAGATGGATCTCAGAAGAGCATGATCAGTGGGAATAAAAACTCAGCGGACGGAGTTCGAGCCTTCCAAACCGCATCTTTTGTGGCACTTGGAGACGGGTTGTTGGCGAAAGGGATCGGATTCAGAAACACTGCAGGTCCAGAAAAGCAGCAGGCCGTAGCCGCCAGAGTCCAGGGCGACAGAGCCATATTCGTGAACTGTCGGTTCGAGGGGTTCCAAGACACGTTGTACGCGCAAGCCCATCGGCAGTTCTACAGGAGCTGCTTGATTACAGGCACAGTGGACATCATCTTCGGGGACGCGGCGGCCATTTTCCAAAATTGTAATTTCATGTTGAAAAAGCCGTTGGATAAGCAAGCGAACGTAGTAACGGCACAGGGGAGAAGTGACAGACATGAAACCACGGCCACTGTGCTGCAAAACTGCAAGATATTGCCGGACAAGACGCTGGAGGGTGTGAAGGCGCAGTTCAAAAGCTACTTGGGGAGGCCTCGGAATGAGTACTCCAGGACCATAGTAATGGAGTCCGAAATTGAAGATATCATTCACCCCGATGGGTGGATGCCTTGGGACGGGGACTTTGCACTCAAAACCTTGTACTATGCTGAGTTTAACAACAAAGGGCCTGGGGCTAAGACCGATGCCAGAGTGAAATGGCCTGGTTTTAAGGTGATTGATAAGGAAGAGGCTGCAAAGTTTACGATAGGAACTTTTCTAGACGTGGATTGGATCCAGAGCTCCTCGGTTTCTGTTCATGTCGGCCTGTTTTGA
BLAST of CmoCh06G000100 vs. Swiss-Prot
Match: PME45_ARATH (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=PME45 PE=2 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 1.3e-164
Identity = 311/615 (50.57%), Postives = 410/615 (66.67%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKS-----TT 60
           M FQDFD+I ER  AER RKFR R+I+ VVS  ++   ++   F  V+   K+     TT
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTT 60

Query: 61  RDGAGSGSSRSKGSSKDHATSDVKQVSRAQ--KMITMICNSTDYKAKCESTLKEGVETHP 120
            + +    ++S+  S    +S  + V   Q  K+I  +CNST YK  C++TLK   +   
Sbjct: 61  NNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDT 120

Query: 121 DSSDPKDLIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVS 180
             +DP+ L+K AI AV+ +++   K+  ++     ++K A   CKLL++EA EEL  S+ 
Sbjct: 121 PQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELGTSMK 180

Query: 181 ELSRKDLGKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSL 240
            ++  ++     K  PDL++WLSAVMSY  TCVDGF  GK KTE+     + + LTSNSL
Sbjct: 181 RINDSEVNNFA-KIVPDLDSWLSAVMSYQETCVDGFEEGKLKTEIRKNFNSSQVLTSNSL 240

Query: 241 AMISQVATFFSTFEKSDGGGAATRRRLMAK------DGLPSWMTSYERRMLKRDAEDDKP 300
           AMI  +  + S+  K       TR  L A+      D + SW+++ ERRMLK  A D K 
Sbjct: 241 AMIKSLDGYLSSVPK-----VKTRLLLEARSSAKETDHITSWLSNKERRMLK--AVDVKA 300

Query: 301 T-PNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGD 360
             PN  VAKDGSGNF TIN AL AMPAKY GRY IY+K GIY+E+V+I KK  NVTM GD
Sbjct: 301 LKPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGD 360

Query: 361 GSQKSMISGNKNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRA 420
           GSQK++++GNK+ A  +R F TA+FVA G+G +A+ +GFRNTAGPE  QAVA RVQ DR+
Sbjct: 361 GSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRS 420

Query: 421 IFVNCRFEGFQDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANV 480
           +F+NCRFEG+QDTLYA  HRQ+YRSC+I GTVD IFGDAAAIFQNC+  ++K L  Q N 
Sbjct: 421 VFLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNT 480

Query: 481 VTAQGRSDRHETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDII 540
           VTAQGR D+ +TT  V+ NC + P++ L+ VKAQFKSYLGRP   +SRT+VMES IED+I
Sbjct: 481 VTAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVI 540

Query: 541 HPDGWMPW-DGDFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDV 600
            P GW+ W + DFA+ TL YAE+ N GP   T ARVKWPGF+V++KEEA KFT+G FL  
Sbjct: 541 DPVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQG 600

BLAST of CmoCh06G000100 vs. Swiss-Prot
Match: PME13_ARATH (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana GN=PME13 PE=2 SV=2)

HSP 1 Score: 558.5 bits (1438), Expect = 8.7e-158
Identity = 304/623 (48.80%), Postives = 410/623 (65.81%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVS---------PPT 60
           M FQDFD+I ER  A R RKFR R+I+  VS  +++  +V   F  V+            
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  KSTTRDGAGSGSSRSKGSSKDHAT----SDVKQVSRAQ--------KMITMICNSTDYKA 120
           ++   + +GSG++  K S K   +    S    VS AQ        K+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

Query: 121 KCESTLKEGVETHPDSSDPKDLIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKL 180
            CE TLK   +      +P   +K AI AV+ +++  L+K  ++     ++K A E CKL
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180

Query: 181 LLEEAVEELDRSVSELSRKDLGKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEME 240
           L+E+A EE   S+++++  ++    EK  PDL +WLSAVMSY  TC+DGF  G  K+E++
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSF-EKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVK 240

Query: 241 ATLKAGKELTSNSLAMISQVATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSYERRMLK 300
            ++ + + LTSNSLA+I       S   K         R L+  D +PSW+++ +RRML+
Sbjct: 241 TSVNSSQVLTSNSLALIKTFTENLSPVMK------VVERHLL--DDIPSWVSNDDRRMLR 300

Query: 301 RDAEDDKPT-PNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKM 360
             A D K   PN  VAKDGSG+F TIN+AL AMP KY+GRY+IYVK+GIY+E V + KK 
Sbjct: 301 --AVDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKK 360

Query: 361 ANVTMYGDGSQKSMISGNKNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVA 420
           AN+TM GDGSQK++++GNK+ A  +R F TA+FVA G+G +A+ +GFRNTAGPE  QAVA
Sbjct: 361 ANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVA 420

Query: 421 ARVQGDRAIFVNCRFEGFQDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKK 480
            RVQ DR+IF+NCRFEG+QDTLYA  HRQ+YRSC+I GT+D IFGDAAAIFQNCN  ++K
Sbjct: 421 IRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRK 480

Query: 481 PLDKQANVVTAQGRSDRHETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVM 540
            L  Q N VTAQGR D+ +TT  V+ NCKI  ++ L+ VK ++KSYLGRP   YSRTI+M
Sbjct: 481 GLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIM 540

Query: 541 ESEIEDIIHPDGWMPW-DGDFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKF 600
           ES+IE++I P GW+ W + DFA+ TLYYAE+NNKG    T +RVKWPGFKVI+KEEA  +
Sbjct: 541 ESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNY 600

BLAST of CmoCh06G000100 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.5e-120
Identity = 241/590 (40.85%), Postives = 349/590 (59.15%), Query Frame = 1

Query: 16  ERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAGSGSSRSKGSSKDHAT 75
           E A++ +  VII++ S  LI ++V   + V V+        D AG               
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKS------DNAGD-------------- 67

Query: 76  SDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKDLIKLAIAAVSTEVNS 135
              ++++ + K I  +C  TDYK  CE TL++  +   D+SDP +L+K A  A   +++ 
Sbjct: 68  ---EEITTSVKAIKDVCAPTDYKETCEDTLRKDAK---DTSDPLELVKTAFNATMKQISD 127

Query: 136 ALKKASAMNF--PTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDLGKLTEKTTPDLNN 195
             KK+  M      P  K A + CK L++ A+ EL +S  EL + +  K+ E     L  
Sbjct: 128 VAKKSQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVK-LRI 187

Query: 196 WLSAVMSYHATCVDGFPG--GKSKTEMEATLKAGKELTSNSLAMISQVATFFSTFEKSDG 255
           WLSA +S+  TC+DGF G  G +   ++  LK   +LT N LAM+++++ +    +  + 
Sbjct: 188 WLSATISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPE- 247

Query: 256 GGAATRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGNFKTINEALAAM 315
                 RRL++++  PSWM +  RR+L     + KP  ++VVA+DGSG +KTINEAL  +
Sbjct: 248 ---MNSRRLLSQE-FPSWMDARARRLLNAPMSEVKP--DIVVAQDGSGQYKTINEALNFV 307

Query: 316 PAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSADGVRAFQTASF 375
           P K +  +V+++KEGIY+E V + + M ++   GDG  K++ISG+K+  DG+  ++TA+ 
Sbjct: 308 PKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATV 367

Query: 376 VALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTLYAQAHRQFYRS 435
             +GD  +AK I F NTAG  K QAVA RV  D +IF NC+F+G+QDTLYA +HRQFYR 
Sbjct: 368 AIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRD 427

Query: 436 CLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTATVLQNCKILPD 495
           C I+GT+D +FGDAAA+FQNC  +++KPL  QA  +TA GR D  E+T  VLQ C I+ +
Sbjct: 428 CTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGE 487

Query: 496 KTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFALKTLYYAEFNNK 555
                VK Q K+YLGRP  EYSRTI+M + I D + P+GW PW G+F L TL+Y+E  N 
Sbjct: 488 PDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNT 547

Query: 556 GPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVD-WIQSSSVSVHVGLF 601
           GPGA    RV WPG K +  EE  KFT   ++  D WI    V   +GLF
Sbjct: 548 GPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLF 563

BLAST of CmoCh06G000100 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 9.7e-117
Identity = 242/583 (41.51%), Postives = 339/583 (58.15%), Query Frame = 1

Query: 22  RNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAGSGSSRSKGSSKDHATSDVKQV 81
           + + IIA V T L++++VVA                    G + S+ +S       V Q+
Sbjct: 9   KKKCIIAGVITALLVLMVVAV-------------------GITTSRNTSHSEKIVPV-QI 68

Query: 82  SRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKDLIKLAIAAVSTEVNSALKKAS 141
             A   +  +C  TDYK  C ++L   ++  PDS+ P DLIKL        +  ++KKAS
Sbjct: 69  KTATTAVEAVCAPTDYKETCVNSL---MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKAS 128

Query: 142 ----AMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDLGKLTEKTTPDLNNWLSA 201
               A      + KGA E C+ L+ +A ++L + +       + ++ E    DL  WLS 
Sbjct: 129 VELTAKAANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQI-EDFVEDLRVWLSG 188

Query: 202 VMSYHATCVDGFPGGKSKT--EMEATLKAGKELTSNSLAMISQVATFFSTFEKSDGGGAA 261
            ++Y  TC+D F    SK   +M+   K  +ELTSN LAMI+ ++     F  +   G  
Sbjct: 189 SIAYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDL 248

Query: 262 ---TRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGNFKTINEALAAMP 321
               R+ L A+DG+PSW+    RR++   A       NVVVA DGSG +KTINEAL A+P
Sbjct: 249 GKYARKLLSAEDGIPSWVGPNTRRLM---ATKGGVKANVVVAHDGSGQYKTINEALNAVP 308

Query: 322 AKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSADG-VRAFQTASF 381
                 +VIY+K+G+Y E V +TKKM +VT  GDG  K+ I+G+ N   G V+ + TA+ 
Sbjct: 309 KANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATV 368

Query: 382 VALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTLYAQAHRQFYRS 441
              GD   AK IGF NTAGPE  QAVA RV  D A+F NC+ +G+QDTLY  +HRQF+R 
Sbjct: 369 AINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRD 428

Query: 442 CLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTATVLQNCKILPD 501
           C ++GTVD IFGD   + QNCN +++KP+  Q+ ++TAQGRSD+ E+T  VLQNC I  +
Sbjct: 429 CTVSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGE 488

Query: 502 KTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFALKTLYYAEFNNK 561
                VK+  K+YLGRP  E+SRTI+M + I+D+I P GW+PW+GDFAL TLYYAE+ N 
Sbjct: 489 PAYIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENN 548

Query: 562 GPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVD-WIQSSSV 594
           GPG+    RVKWPG K +  ++A +FT   FL  + WI  + V
Sbjct: 549 GPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRV 564

BLAST of CmoCh06G000100 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 421.8 bits (1083), Expect = 1.3e-116
Identity = 234/585 (40.00%), Postives = 339/585 (57.95%), Query Frame = 1

Query: 20  KFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAGSGSSRSKGSSKDHATSDVK 79
           K + R I+  +S+ L++ +VVA                  G   ++  G SK  A     
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVT---------------VGVSLNKHDGDSKGKA----- 69

Query: 80  QVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKDLIKLAIAAVSTEVNSALKK 139
           +V+ + K +  +C  TDY+  CE TL   ++   +++DP +L+K A      ++  A KK
Sbjct: 70  EVNASVKAVKDVCAPTDYRKTCEDTL---IKNGKNTTDPMELVKTAFNVTMKQITDAAKK 129

Query: 140 ASAMNFPTPEEKG--AFEDCKLLLEEAVEELDRSVSELSRKDLGKLTEKTTPDLNNWLSA 199
           +  +     + +   A + CK L++ A++EL  S  EL + +   L E    +L  WLSA
Sbjct: 130 SQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALI-NLRIWLSA 189

Query: 200 VMSYHATCVDGFPG--GKSKTEMEATLKAGKELTSNSLAMISQVATFFSTFEKSDGGGAA 259
            +S+  TC++GF G  G +   M+  LK   ELT N LA+IS+++ F    +        
Sbjct: 190 AISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIP----GL 249

Query: 260 TRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGNFKTINEALAAMPAKY 319
             RRL+A +G PSW+    R++L+  A      P++VVA+DGSG +KTINEAL  +P K 
Sbjct: 250 NSRRLLA-EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKR 309

Query: 320 DGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSADGVRAFQTASFVALG 379
           +  +V+++K G+Y+E V + K M+++   GDG  K++ISGNKN  DG+  ++TA+   +G
Sbjct: 310 NTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVG 369

Query: 380 DGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTLYAQAHRQFYRSCLIT 439
           +  +AK IGF NTAG  K QAVA RVQ D +IF NCRF+G+QDTLY  +HRQF+R C I+
Sbjct: 370 NYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTIS 429

Query: 440 GTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTATVLQNCKILPDKTLE 499
           GT+D +FGDAAA+FQNC  +++KPL  QA  +TA GR D  E+T  V Q C I  +    
Sbjct: 430 GTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYL 489

Query: 500 GVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFALKTLYYAEFNNKGPGA 559
            VK   K+YLGRP  EYSRTI+M + I D + P GW PW GDF LKTL+Y+E  N GPG+
Sbjct: 490 AVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGS 549

Query: 560 KTDARVKWPGFKVIDKEEAAKFTIGTFLD-VDWIQSSSVSVHVGL 600
               RV W G K + +E+  KFT   ++   DWI    V    GL
Sbjct: 550 ALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmoCh06G000100 vs. TrEMBL
Match: A0A0A0KX33_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G630740 PE=4 SV=1)

HSP 1 Score: 921.4 bits (2380), Expect = 5.7e-265
Identity = 464/600 (77.33%), Postives = 522/600 (87.00%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           MVFQDFDQISERRRAERARKFR RVIIAVVS  +IL++V AAVFVQVS    +T  D +G
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSSKSTTKNDDSG 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
           S S    G+SK     + KQVSR +KMITMICNSTDYK KCESTLK+GV T P+SSDPKD
Sbjct: 61  SSS----GNSKQPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPNSSDPKD 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
           LIKLAI+A + EV SA+KKAS  NF TPEEKGAFEDCK+LLE+A+EEL+ S+SE+++K++
Sbjct: 121 LIKLAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSEVNKKNM 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
           GKLT KTTP+LNNWLSAVMSYH TCVDGFP GK K+++E  +KAGKELTSNSLAMISQVA
Sbjct: 181 GKLTAKTTPNLNNWLSAVMSYHETCVDGFPEGKMKSDIEKVVKAGKELTSNSLAMISQVA 240

Query: 241 TFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGN 300
           +FFSTFE  +  GAA+RRRLM  +G+P+WM   +RRMLK  A  +KP PNVVVAKDGSG 
Sbjct: 241 SFFSTFEMPE--GAASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVVVAKDGSGE 300

Query: 301 FKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSA 360
           FKTINEALAAMPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISG+KN  
Sbjct: 301 FKTINEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFV 360

Query: 361 DGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTL 420
           DGVR FQTA+FVALG+G L + IGFRN AGPEK QAVAARVQ DRAIFVNCRFEG+QDTL
Sbjct: 361 DGVRTFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTL 420

Query: 421 YAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTA 480
           Y QAHRQFYRSCLITGT+D IFGDAAAIFQNCN M++KPLD Q N+VTAQGR+D+HETT 
Sbjct: 421 YTQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGRTDKHETTG 480

Query: 481 TVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFAL 540
            VLQNCKILPDKTLE VK+QFKSYLGRP  E+SRTIVMES IED+IHPDGWM W+GDFAL
Sbjct: 481 IVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWMAWEGDFAL 540

Query: 541 KTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHVGLF 600
           KTLYYAEFNNKGPGAKTDARVKWPG+KVIDK+EAAKFTIGTFL++DWI+S+S  VHVGLF
Sbjct: 541 KTLYYAEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 594

BLAST of CmoCh06G000100 vs. TrEMBL
Match: A0A067KCM7_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_07552 PE=4 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 2.9e-200
Identity = 366/604 (60.60%), Postives = 450/604 (74.50%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           MVFQDFDQISERRR ER +KFR R+ IA ++  L+LV++   VF  +S   K+T R  + 
Sbjct: 1   MVFQDFDQISERRRLEREQKFRKRITIAAITIVLLLVIIGCGVFAIISE-NKNTKR--SK 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
             S  SKGS+KD        V++  ++I  ICN TDYK  C S+L+  V+  P S++PK 
Sbjct: 61  ESSQESKGSAKD--------VAQVDRVIRRICNGTDYKDTCVSSLESEVKKDPSSANPKA 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
           +++LAI A   E+   LKKAS+  F +P+EK AFEDCK+L+++A EEL  S++     D+
Sbjct: 121 ILRLAIEATDDEMGVVLKKASSFKFESPQEKAAFEDCKVLIKDAKEELKESLTHAG-VDI 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
            KL E   P+LNN LSAVMSY  TC+DGFP GK K++ME T  A KELTSNSLAM+S + 
Sbjct: 181 KKLAENE-PELNNLLSAVMSYQQTCIDGFPDGKLKSDMEKTFNASKELTSNSLAMVSSLT 240

Query: 241 TFFSTFEKSDGGGA--ATRRRL--MAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKD 300
           +F S+F + +      AT+  L  M KD LPSW+++ +RR+LK D + DKPTPNV+VAKD
Sbjct: 241 SFLSSFGEINHSRRLLATKPNLSSMDKDELPSWISNEDRRILKAD-DSDKPTPNVIVAKD 300

Query: 301 GSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGN 360
           GSG FKTI+EAL AMP KY GRY+IYVK G+Y+E V + KKM N+TMYGDGSQKS+I+GN
Sbjct: 301 GSGKFKTISEALKAMPKKYKGRYIIYVKAGVYDEVVTVEKKMENITMYGDGSQKSIITGN 360

Query: 361 KNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGF 420
           KN  DGV+ F+TASFVALG+G +AK IGFRNTAGPEK QAVAARVQ DR IF+NCRFEG+
Sbjct: 361 KNFKDGVQTFRTASFVALGNGFMAKAIGFRNTAGPEKHQAVAARVQADRTIFLNCRFEGY 420

Query: 421 QDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRH 480
           QDTLY Q HRQ+YRSC+I GT+D IFGDAAAI QNC  +++KPL+ Q N+VTAQGR D H
Sbjct: 421 QDTLYVQTHRQYYRSCVIAGTIDFIFGDAAAILQNCQIVIRKPLENQQNIVTAQGRIDPH 480

Query: 481 ETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDG 540
           ETT  VLQNC+I PDK+L   KA+FKSYLGRP  EYSRTIVMES IED+IHPDGW+ W+G
Sbjct: 481 ETTGIVLQNCRIQPDKSLVPDKAKFKSYLGRPWKEYSRTIVMESTIEDLIHPDGWLAWEG 540

Query: 541 DFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVH 600
           +  LKTLYYAEFNNKGPGA T ARVKWPG+ VIDKEEA K+TI  F+  DWI      VH
Sbjct: 541 EKGLKTLYYAEFNNKGPGASTKARVKWPGYHVIDKEEANKYTIEPFIQGDWINDMGAPVH 590

BLAST of CmoCh06G000100 vs. TrEMBL
Match: A0A061GJT0_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_037445 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 1.2e-198
Identity = 361/605 (59.67%), Postives = 452/605 (74.71%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           MVFQDFD I ERRR ER RKFR RV IAVV+T +++ LV A +FV VS  TK+  + G  
Sbjct: 1   MVFQDFDLIQERRRLERQRKFRKRVGIAVVTTCVVIGLVAAGIFVIVS--TKNKEQQGTS 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
           +       ++K H     KQVSR++K+I  ICN T YK  CESTLK+ V+ HP+++ PKD
Sbjct: 61  A-------NNKAHQAPSTKQVSRSEKLINTICNGTSYKESCESTLKKAVKEHPNTAQPKD 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
           L+K +I+A + E++ A  KAS+  F +P EK AF  CK ++  A EEL  ++ ++  KD 
Sbjct: 121 LLKSSISATADELDKAFTKASSFQFNSPGEKQAFNVCKEVMANAKEELATAIGKVGDKDT 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
           GKL   ++ +LNNWLSAVMSY  TC+D F  GK KT++++TL + +ELTSNSLAM  Q++
Sbjct: 181 GKLL--SSGELNNWLSAVMSYQETCIDSFADGKLKTDIKSTLNSSQELTSNSLAMARQLS 240

Query: 241 TFFSTFEKSDGGGAATRRRLMA------KDGLPSWMTSYERRMLKRDAEDDKPTPNVVVA 300
           +  S+  +     AA+R  L +      KDGLP+W+   ERR+LK  A+ +KPTPNV VA
Sbjct: 241 SHVSSVMEMP---AASRHLLESTSPSVDKDGLPNWLNHDERRLLK-GADVEKPTPNVTVA 300

Query: 301 KDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMIS 360
           KDGSGNF TI+EALAAMP KYDGRYVIYVK GIYEETV++TKKM N+T+YGDGSQK++++
Sbjct: 301 KDGSGNFSTISEALAAMPQKYDGRYVIYVKAGIYEETVLVTKKMVNLTIYGDGSQKTIVT 360

Query: 361 GNKNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFE 420
           GNKN  DGV  + TA+FVA GDG LAK +GFRNTAGPEK QAVAARV  DRAIF+NCRFE
Sbjct: 361 GNKNYVDGVPTYLTATFVASGDGFLAKAMGFRNTAGPEKHQAVAARVDADRAIFLNCRFE 420

Query: 421 GFQDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSD 480
           G+QDTLY Q HRQFYRSC++ GT+D IFGDAAA+FQNC   ++KP+D Q N+VTAQGR D
Sbjct: 421 GYQDTLYVQTHRQFYRSCVVAGTIDFIFGDAAAVFQNCLIYVRKPMDNQKNIVTAQGRKD 480

Query: 481 RHETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPW 540
           + ETT  VLQNCKILPD +    K+QFKSYLGRP  EYSRTIVMES IED+I P GW+ W
Sbjct: 481 KFETTGIVLQNCKILPDDSFTSFKSQFKSYLGRPWKEYSRTIVMESLIEDLIDPAGWLEW 540

Query: 541 DGDFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVS 600
           +G+FAL TLYYAE+NN GPGAKTDARVKWPG KVI+K+EA KFT+ TFL   WI+++   
Sbjct: 541 EGNFALNTLYYAEYNNNGPGAKTDARVKWPGRKVINKDEAMKFTVETFLQGTWIKATGAP 590

BLAST of CmoCh06G000100 vs. TrEMBL
Match: B9RR24_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_0708480 PE=4 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 3.5e-198
Identity = 360/609 (59.11%), Postives = 444/609 (72.91%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           MVFQDFDQISERRR ER +KFR RV IA V+ F +LV+V A VF  +S    +     + 
Sbjct: 1   MVFQDFDQISERRRLERQQKFRKRVTIAAVAVFALLVVVAAGVFAIIS----NNNHKQSQ 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
              S+S  S K     D  +VS   K+I  +CN+T YK  C ++LK+ VE  P S+ PKD
Sbjct: 61  QQHSKSTQSQKP----DNSEVSHLGKVIDNVCNATTYKETCHTSLKKAVEKDPSSAHPKD 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
           ++KLAI +   E    L+K  +  F +P EK AFEDCK L+++A EEL++S+S     D 
Sbjct: 121 VLKLAIGSTEDEFARILEKVKSFKFESPREKAAFEDCKELIDDAKEELNKSISSAGG-DT 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
           GKL  K   DLNNWLSAVMSY  TC+DGFP GK K++ME T K  KELTSNSLAM+S++ 
Sbjct: 181 GKLL-KNEADLNNWLSAVMSYQQTCIDGFPEGKLKSDMEKTFKEAKELTSNSLAMVSELT 240

Query: 241 TFFSTFEKSDGGGAATRRRLMAK---------DGLPSWMTSYERRMLKRDAEDDKPTPNV 300
            F + F           RRL+AK         DG+PSW++  +RR+LK  ++ DKPTPNV
Sbjct: 241 AFLTAFSVPKPS-----RRLLAKESNTSSFGEDGIPSWISPEDRRILK-GSDGDKPTPNV 300

Query: 301 VVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKS 360
            VAKDGSG FKTI++ALAAMP KY GRYVIYVK GIY+ETV +TK M NVT+YGDGSQKS
Sbjct: 301 TVAKDGSGQFKTISDALAAMPEKYQGRYVIYVKAGIYDETVTVTKNMVNVTIYGDGSQKS 360

Query: 361 MISGNKNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNC 420
           +++G+KN ADGV+ F+TA+F ALGDG +AK +GFRNTAGP+K QAVA RVQ DR+IF+NC
Sbjct: 361 IVTGSKNFADGVQTFRTATFAALGDGFIAKAMGFRNTAGPQKHQAVAVRVQADRSIFLNC 420

Query: 421 RFEGFQDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQG 480
           RFEG+QDTLYAQ HRQFYRSC+I+GT+D IFGDA AIFQNC  +++KP++ Q N+VTAQG
Sbjct: 421 RFEGYQDTLYAQTHRQFYRSCVISGTIDFIFGDATAIFQNCLILVRKPMENQQNIVTAQG 480

Query: 481 RSDRHETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGW 540
           R D HETT  V+QNC+I PDK L   KA  KSYLGRP  +YSRTIVMES IED IHPDGW
Sbjct: 481 RIDSHETTGIVIQNCRIQPDKDLIPAKATVKSYLGRPWKDYSRTIVMESTIEDFIHPDGW 540

Query: 541 MPWDGDFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSS 600
           + W+G+  LKTLYYAEFNNKGPG+KTDARVKWPG+ VID++EA K+T+  FL  DWI ++
Sbjct: 541 LAWEGEKGLKTLYYAEFNNKGPGSKTDARVKWPGYHVIDQQEANKYTVKPFLQGDWITAA 593

BLAST of CmoCh06G000100 vs. TrEMBL
Match: U5G7D7_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0006s13690g PE=4 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 6.0e-198
Identity = 356/607 (58.65%), Postives = 440/607 (72.49%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           MVFQDFDQ+SERR+ ER +K R ++II  VS+    V+V A VF  VS    S+     G
Sbjct: 1   MVFQDFDQLSERRKLERQQKLRKKIIIGSVSSIAFFVIVGAGVFALVSNHNISSPGSNGG 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
           S S+ S+           K  S   ++I  +CN+T Y+  C++TL++G+   P S  PKD
Sbjct: 61  SPSTVSQ------PVESAKPTSHVARVIKTVCNATTYQDTCQNTLEKGMRKDPSSVQPKD 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
           L+K+AI A   E+   LKKAS+  F  P EK AF+DC  L+E+A EEL   +  +   D+
Sbjct: 121 LLKIAIKAADKEIEKVLKKASSFKFDKPREKAAFDDCLELIEDAKEELKHCIDRVGN-DI 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
           GKLT K  PDLNNWLSAVMSY  TC+DGFP GK K++ME T KA +ELTSNSLAM+S +A
Sbjct: 181 GKLT-KNAPDLNNWLSAVMSYQQTCIDGFPEGKLKSDMEKTFKAARELTSNSLAMVSSLA 240

Query: 241 TFFSTFEKSDGGGAATRRRL--------MAKDGLPSWMTSYERRMLKRDAEDDKPTPNVV 300
           +F   F  S   G   RR L        + KDGLP W TS+E R + + A  DKP P+V 
Sbjct: 241 SFLKNFSFS---GTLNRRLLAEEYNSPSLDKDGLPGW-TSHEDRRILKGANQDKPKPHVT 300

Query: 301 VAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSM 360
           VAKDGSG+FKTI+EALAAMPAKY+GRYVI+VK+GIY+ETV +TKKM N+TMYGDGSQK++
Sbjct: 301 VAKDGSGDFKTISEALAAMPAKYEGRYVIFVKQGIYDETVTVTKKMVNITMYGDGSQKTI 360

Query: 361 ISGNKNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCR 420
           ++GNKN ADGV+ F+TA+F  LG+G L K +GFRNTAGPEK QAVA RVQ DRAIF+NCR
Sbjct: 361 VTGNKNFADGVQTFRTATFAVLGEGFLCKAMGFRNTAGPEKHQAVAIRVQADRAIFLNCR 420

Query: 421 FEGFQDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGR 480
           FEG+QDTLYAQ HRQFYRSC+ITGTVD IFGDAAAIFQNC   ++KPL+ Q N+VTAQGR
Sbjct: 421 FEGYQDTLYAQTHRQFYRSCVITGTVDFIFGDAAAIFQNCLITVRKPLENQQNIVTAQGR 480

Query: 481 SDRHETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWM 540
            D HETT  VLQNC+I PDK L  VK + +SYLGRP  E+SRT++M+S I D IHPDGW+
Sbjct: 481 IDGHETTGIVLQNCRIEPDKGLVPVKTKIRSYLGRPWKEFSRTVIMDSTIGDFIHPDGWL 540

Query: 541 PWDGDFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSS 600
           PW GDF LKTLYYAE+NNKG GA+T+AR+KW G+ +I KEEA KFT+ TF  VDWI ++ 
Sbjct: 541 PWQGDFGLKTLYYAEYNNKGIGAQTNARIKWRGYHIIKKEEAMKFTVETFYQVDWISATG 595

BLAST of CmoCh06G000100 vs. TAIR10
Match: AT4G33230.1 (AT4G33230.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 581.3 bits (1497), Expect = 7.1e-166
Identity = 311/615 (50.57%), Postives = 410/615 (66.67%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKS-----TT 60
           M FQDFD+I ER  AER RKFR R+I+ VVS  ++   ++   F  V+   K+     TT
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTT 60

Query: 61  RDGAGSGSSRSKGSSKDHATSDVKQVSRAQ--KMITMICNSTDYKAKCESTLKEGVETHP 120
            + +    ++S+  S    +S  + V   Q  K+I  +CNST YK  C++TLK   +   
Sbjct: 61  NNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDT 120

Query: 121 DSSDPKDLIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVS 180
             +DP+ L+K AI AV+ +++   K+  ++     ++K A   CKLL++EA EEL  S+ 
Sbjct: 121 PQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELGTSMK 180

Query: 181 ELSRKDLGKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSL 240
            ++  ++     K  PDL++WLSAVMSY  TCVDGF  GK KTE+     + + LTSNSL
Sbjct: 181 RINDSEVNNFA-KIVPDLDSWLSAVMSYQETCVDGFEEGKLKTEIRKNFNSSQVLTSNSL 240

Query: 241 AMISQVATFFSTFEKSDGGGAATRRRLMAK------DGLPSWMTSYERRMLKRDAEDDKP 300
           AMI  +  + S+  K       TR  L A+      D + SW+++ ERRMLK  A D K 
Sbjct: 241 AMIKSLDGYLSSVPK-----VKTRLLLEARSSAKETDHITSWLSNKERRMLK--AVDVKA 300

Query: 301 T-PNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGD 360
             PN  VAKDGSGNF TIN AL AMPAKY GRY IY+K GIY+E+V+I KK  NVTM GD
Sbjct: 301 LKPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGD 360

Query: 361 GSQKSMISGNKNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRA 420
           GSQK++++GNK+ A  +R F TA+FVA G+G +A+ +GFRNTAGPE  QAVA RVQ DR+
Sbjct: 361 GSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRS 420

Query: 421 IFVNCRFEGFQDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANV 480
           +F+NCRFEG+QDTLYA  HRQ+YRSC+I GTVD IFGDAAAIFQNC+  ++K L  Q N 
Sbjct: 421 VFLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNT 480

Query: 481 VTAQGRSDRHETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDII 540
           VTAQGR D+ +TT  V+ NC + P++ L+ VKAQFKSYLGRP   +SRT+VMES IED+I
Sbjct: 481 VTAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVI 540

Query: 541 HPDGWMPW-DGDFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDV 600
            P GW+ W + DFA+ TL YAE+ N GP   T ARVKWPGF+V++KEEA KFT+G FL  
Sbjct: 541 DPVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQG 600

BLAST of CmoCh06G000100 vs. TAIR10
Match: AT2G26450.1 (AT2G26450.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 558.5 bits (1438), Expect = 4.9e-159
Identity = 304/623 (48.80%), Postives = 410/623 (65.81%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVS---------PPT 60
           M FQDFD+I ER  A R RKFR R+I+  VS  +++  +V   F  V+            
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  KSTTRDGAGSGSSRSKGSSKDHAT----SDVKQVSRAQ--------KMITMICNSTDYKA 120
           ++   + +GSG++  K S K   +    S    VS AQ        K+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

Query: 121 KCESTLKEGVETHPDSSDPKDLIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKL 180
            CE TLK   +      +P   +K AI AV+ +++  L+K  ++     ++K A E CKL
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180

Query: 181 LLEEAVEELDRSVSELSRKDLGKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEME 240
           L+E+A EE   S+++++  ++    EK  PDL +WLSAVMSY  TC+DGF  G  K+E++
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSF-EKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVK 240

Query: 241 ATLKAGKELTSNSLAMISQVATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSYERRMLK 300
            ++ + + LTSNSLA+I       S   K         R L+  D +PSW+++ +RRML+
Sbjct: 241 TSVNSSQVLTSNSLALIKTFTENLSPVMK------VVERHLL--DDIPSWVSNDDRRMLR 300

Query: 301 RDAEDDKPT-PNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKM 360
             A D K   PN  VAKDGSG+F TIN+AL AMP KY+GRY+IYVK+GIY+E V + KK 
Sbjct: 301 --AVDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKK 360

Query: 361 ANVTMYGDGSQKSMISGNKNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVA 420
           AN+TM GDGSQK++++GNK+ A  +R F TA+FVA G+G +A+ +GFRNTAGPE  QAVA
Sbjct: 361 ANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVA 420

Query: 421 ARVQGDRAIFVNCRFEGFQDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKK 480
            RVQ DR+IF+NCRFEG+QDTLYA  HRQ+YRSC+I GT+D IFGDAAAIFQNCN  ++K
Sbjct: 421 IRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRK 480

Query: 481 PLDKQANVVTAQGRSDRHETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVM 540
            L  Q N VTAQGR D+ +TT  V+ NCKI  ++ L+ VK ++KSYLGRP   YSRTI+M
Sbjct: 481 GLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIM 540

Query: 541 ESEIEDIIHPDGWMPW-DGDFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKF 600
           ES+IE++I P GW+ W + DFA+ TLYYAE+NNKG    T +RVKWPGFKVI+KEEA  +
Sbjct: 541 ESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNY 600

BLAST of CmoCh06G000100 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 434.9 bits (1117), Expect = 8.2e-122
Identity = 241/590 (40.85%), Postives = 349/590 (59.15%), Query Frame = 1

Query: 16  ERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAGSGSSRSKGSSKDHAT 75
           E A++ +  VII++ S  LI ++V   + V V+        D AG               
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKS------DNAGD-------------- 67

Query: 76  SDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKDLIKLAIAAVSTEVNS 135
              ++++ + K I  +C  TDYK  CE TL++  +   D+SDP +L+K A  A   +++ 
Sbjct: 68  ---EEITTSVKAIKDVCAPTDYKETCEDTLRKDAK---DTSDPLELVKTAFNATMKQISD 127

Query: 136 ALKKASAMNF--PTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDLGKLTEKTTPDLNN 195
             KK+  M      P  K A + CK L++ A+ EL +S  EL + +  K+ E     L  
Sbjct: 128 VAKKSQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVK-LRI 187

Query: 196 WLSAVMSYHATCVDGFPG--GKSKTEMEATLKAGKELTSNSLAMISQVATFFSTFEKSDG 255
           WLSA +S+  TC+DGF G  G +   ++  LK   +LT N LAM+++++ +    +  + 
Sbjct: 188 WLSATISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPE- 247

Query: 256 GGAATRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGNFKTINEALAAM 315
                 RRL++++  PSWM +  RR+L     + KP  ++VVA+DGSG +KTINEAL  +
Sbjct: 248 ---MNSRRLLSQE-FPSWMDARARRLLNAPMSEVKP--DIVVAQDGSGQYKTINEALNFV 307

Query: 316 PAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSADGVRAFQTASF 375
           P K +  +V+++KEGIY+E V + + M ++   GDG  K++ISG+K+  DG+  ++TA+ 
Sbjct: 308 PKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATV 367

Query: 376 VALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTLYAQAHRQFYRS 435
             +GD  +AK I F NTAG  K QAVA RV  D +IF NC+F+G+QDTLYA +HRQFYR 
Sbjct: 368 AIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRD 427

Query: 436 CLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTATVLQNCKILPD 495
           C I+GT+D +FGDAAA+FQNC  +++KPL  QA  +TA GR D  E+T  VLQ C I+ +
Sbjct: 428 CTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGE 487

Query: 496 KTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFALKTLYYAEFNNK 555
                VK Q K+YLGRP  EYSRTI+M + I D + P+GW PW G+F L TL+Y+E  N 
Sbjct: 488 PDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNT 547

Query: 556 GPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVD-WIQSSSVSVHVGLF 601
           GPGA    RV WPG K +  EE  KFT   ++  D WI    V   +GLF
Sbjct: 548 GPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLF 563

BLAST of CmoCh06G000100 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 422.2 bits (1084), Expect = 5.5e-118
Identity = 242/583 (41.51%), Postives = 339/583 (58.15%), Query Frame = 1

Query: 22  RNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAGSGSSRSKGSSKDHATSDVKQV 81
           + + IIA V T L++++VVA                    G + S+ +S       V Q+
Sbjct: 9   KKKCIIAGVITALLVLMVVAV-------------------GITTSRNTSHSEKIVPV-QI 68

Query: 82  SRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKDLIKLAIAAVSTEVNSALKKAS 141
             A   +  +C  TDYK  C ++L   ++  PDS+ P DLIKL        +  ++KKAS
Sbjct: 69  KTATTAVEAVCAPTDYKETCVNSL---MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKAS 128

Query: 142 ----AMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDLGKLTEKTTPDLNNWLSA 201
               A      + KGA E C+ L+ +A ++L + +       + ++ E    DL  WLS 
Sbjct: 129 VELTAKAANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQI-EDFVEDLRVWLSG 188

Query: 202 VMSYHATCVDGFPGGKSKT--EMEATLKAGKELTSNSLAMISQVATFFSTFEKSDGGGAA 261
            ++Y  TC+D F    SK   +M+   K  +ELTSN LAMI+ ++     F  +   G  
Sbjct: 189 SIAYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDL 248

Query: 262 ---TRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGNFKTINEALAAMP 321
               R+ L A+DG+PSW+    RR++   A       NVVVA DGSG +KTINEAL A+P
Sbjct: 249 GKYARKLLSAEDGIPSWVGPNTRRLM---ATKGGVKANVVVAHDGSGQYKTINEALNAVP 308

Query: 322 AKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSADG-VRAFQTASF 381
                 +VIY+K+G+Y E V +TKKM +VT  GDG  K+ I+G+ N   G V+ + TA+ 
Sbjct: 309 KANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATV 368

Query: 382 VALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTLYAQAHRQFYRS 441
              GD   AK IGF NTAGPE  QAVA RV  D A+F NC+ +G+QDTLY  +HRQF+R 
Sbjct: 369 AINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRD 428

Query: 442 CLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTATVLQNCKILPD 501
           C ++GTVD IFGD   + QNCN +++KP+  Q+ ++TAQGRSD+ E+T  VLQNC I  +
Sbjct: 429 CTVSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGE 488

Query: 502 KTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFALKTLYYAEFNNK 561
                VK+  K+YLGRP  E+SRTI+M + I+D+I P GW+PW+GDFAL TLYYAE+ N 
Sbjct: 489 PAYIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENN 548

Query: 562 GPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVD-WIQSSSV 594
           GPG+    RVKWPG K +  ++A +FT   FL  + WI  + V
Sbjct: 549 GPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRV 564

BLAST of CmoCh06G000100 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 421.8 bits (1083), Expect = 7.2e-118
Identity = 234/585 (40.00%), Postives = 339/585 (57.95%), Query Frame = 1

Query: 20  KFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAGSGSSRSKGSSKDHATSDVK 79
           K + R I+  +S+ L++ +VVA                  G   ++  G SK  A     
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVT---------------VGVSLNKHDGDSKGKA----- 69

Query: 80  QVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKDLIKLAIAAVSTEVNSALKK 139
           +V+ + K +  +C  TDY+  CE TL   ++   +++DP +L+K A      ++  A KK
Sbjct: 70  EVNASVKAVKDVCAPTDYRKTCEDTL---IKNGKNTTDPMELVKTAFNVTMKQITDAAKK 129

Query: 140 ASAMNFPTPEEKG--AFEDCKLLLEEAVEELDRSVSELSRKDLGKLTEKTTPDLNNWLSA 199
           +  +     + +   A + CK L++ A++EL  S  EL + +   L E    +L  WLSA
Sbjct: 130 SQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALI-NLRIWLSA 189

Query: 200 VMSYHATCVDGFPG--GKSKTEMEATLKAGKELTSNSLAMISQVATFFSTFEKSDGGGAA 259
            +S+  TC++GF G  G +   M+  LK   ELT N LA+IS+++ F    +        
Sbjct: 190 AISHEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIP----GL 249

Query: 260 TRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGNFKTINEALAAMPAKY 319
             RRL+A +G PSW+    R++L+  A      P++VVA+DGSG +KTINEAL  +P K 
Sbjct: 250 NSRRLLA-EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKR 309

Query: 320 DGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSADGVRAFQTASFVALG 379
           +  +V+++K G+Y+E V + K M+++   GDG  K++ISGNKN  DG+  ++TA+   +G
Sbjct: 310 NTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVG 369

Query: 380 DGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTLYAQAHRQFYRSCLIT 439
           +  +AK IGF NTAG  K QAVA RVQ D +IF NCRF+G+QDTLY  +HRQF+R C I+
Sbjct: 370 NYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTIS 429

Query: 440 GTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTATVLQNCKILPDKTLE 499
           GT+D +FGDAAA+FQNC  +++KPL  QA  +TA GR D  E+T  V Q C I  +    
Sbjct: 430 GTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYL 489

Query: 500 GVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFALKTLYYAEFNNKGPGA 559
            VK   K+YLGRP  EYSRTI+M + I D + P GW PW GDF LKTL+Y+E  N GPG+
Sbjct: 490 AVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGS 549

Query: 560 KTDARVKWPGFKVIDKEEAAKFTIGTFLD-VDWIQSSSVSVHVGL 600
               RV W G K + +E+  KFT   ++   DWI    V    GL
Sbjct: 550 ALANRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmoCh06G000100 vs. NCBI nr
Match: gi|659121235|ref|XP_008460558.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo])

HSP 1 Score: 933.7 bits (2412), Expect = 1.6e-268
Identity = 474/600 (79.00%), Postives = 528/600 (88.00%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           MVFQDFDQISERRRAERARKFR RV IAVVS  +IL++V AAVFVQVS  +KSTT++G  
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNG-D 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
           SGSS   G+SK     + KQVSR +KMITMICNSTDYK KCESTLK+G+ T P+SSDPKD
Sbjct: 61  SGSST--GNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKD 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
           LIKLAI+A + EV SA+KKAS  NF TPEEKGAFEDCK+LLE+AVEEL+ S+SE+S+K++
Sbjct: 121 LIKLAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNM 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
           GKLT KTTPDLNNWLSAVMSYH TCVDGFP GK K+EME  +KAGKELTSNSLAMISQVA
Sbjct: 181 GKLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVA 240

Query: 241 TFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGN 300
           +FFSTFE  +  GAA+RRRLMA  G+P+WM S ERRMLK  A  +KP PNVVVAKDGSG 
Sbjct: 241 SFFSTFEMPE--GAASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGE 300

Query: 301 FKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSA 360
           FKTINEALAAMPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISG+KN  
Sbjct: 301 FKTINEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFV 360

Query: 361 DGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTL 420
           DGVR FQTA+FVALG+G L + IGFRN AGPEK QAVAARVQ DRAIFVNCRFEG+QDTL
Sbjct: 361 DGVRTFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTL 420

Query: 421 YAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTA 480
           YAQAHRQFYRSCLITGT+D IFGDAAAIFQNCN M++KPLD Q N+VTAQGR+D+HETT 
Sbjct: 421 YAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTG 480

Query: 481 TVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFAL 540
            VLQNCKILPDKTLE VK+QFKSYLGRP  E+SRTIVMES+IED+IHPDGWM W GDFAL
Sbjct: 481 IVLQNCKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFAL 540

Query: 541 KTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHVGLF 600
           KTLYYAEFNN+GPGAKTDARVKWPG+KVIDK+EAAKFTIGTFL++DWI+S+S  VHVGLF
Sbjct: 541 KTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 595

BLAST of CmoCh06G000100 vs. NCBI nr
Match: gi|449449216|ref|XP_004142361.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis sativus])

HSP 1 Score: 921.4 bits (2380), Expect = 8.2e-265
Identity = 464/600 (77.33%), Postives = 522/600 (87.00%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           MVFQDFDQISERRRAERARKFR RVIIAVVS  +IL++V AAVFVQVS    +T  D +G
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSSKSTTKNDDSG 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
           S S    G+SK     + KQVSR +KMITMICNSTDYK KCESTLK+GV T P+SSDPKD
Sbjct: 61  SSS----GNSKQPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPNSSDPKD 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
           LIKLAI+A + EV SA+KKAS  NF TPEEKGAFEDCK+LLE+A+EEL+ S+SE+++K++
Sbjct: 121 LIKLAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSEVNKKNM 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
           GKLT KTTP+LNNWLSAVMSYH TCVDGFP GK K+++E  +KAGKELTSNSLAMISQVA
Sbjct: 181 GKLTAKTTPNLNNWLSAVMSYHETCVDGFPEGKMKSDIEKVVKAGKELTSNSLAMISQVA 240

Query: 241 TFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGN 300
           +FFSTFE  +  GAA+RRRLM  +G+P+WM   +RRMLK  A  +KP PNVVVAKDGSG 
Sbjct: 241 SFFSTFEMPE--GAASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVVVAKDGSGE 300

Query: 301 FKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSA 360
           FKTINEALAAMPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISG+KN  
Sbjct: 301 FKTINEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFV 360

Query: 361 DGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTL 420
           DGVR FQTA+FVALG+G L + IGFRN AGPEK QAVAARVQ DRAIFVNCRFEG+QDTL
Sbjct: 361 DGVRTFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTL 420

Query: 421 YAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTA 480
           Y QAHRQFYRSCLITGT+D IFGDAAAIFQNCN M++KPLD Q N+VTAQGR+D+HETT 
Sbjct: 421 YTQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGRTDKHETTG 480

Query: 481 TVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFAL 540
            VLQNCKILPDKTLE VK+QFKSYLGRP  E+SRTIVMES IED+IHPDGWM W+GDFAL
Sbjct: 481 IVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWMAWEGDFAL 540

Query: 541 KTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHVGLF 600
           KTLYYAEFNNKGPGAKTDARVKWPG+KVIDK+EAAKFTIGTFL++DWI+S+S  VHVGLF
Sbjct: 541 KTLYYAEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 594

BLAST of CmoCh06G000100 vs. NCBI nr
Match: gi|1009142836|ref|XP_015888938.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Ziziphus jujuba])

HSP 1 Score: 720.7 bits (1859), Expect = 2.1e-204
Identity = 360/600 (60.00%), Postives = 444/600 (74.00%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           MVFQDFD+ISERRRAER RKF+ RV I +++  ++LV++ A V V V      TT +   
Sbjct: 1   MVFQDFDKISERRRAERERKFKKRVTIGLITGLVLLVIIAAGVIVLV------TTNNNNN 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
             +S +K SS    ++  + V + +K+IT +C+STDYK +C  +L    +  P+ S PKD
Sbjct: 61  DSNSNNKNSSTTSKSNKGRDVVKVEKVITSVCSSTDYKQRCHDSLTAAAKKQPELSQPKD 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
            IK+AI+A S E+  A  K S   F TPEEK AF DCK+L+E+A+EEL+ S   +     
Sbjct: 121 FIKVAISAASDELTKAFNKTSNFTFNTPEEKAAFGDCKVLMEDALEELEASGKHVGDNAT 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
                  TPDLN+WLSAV SY  TC+DGFP GK KT+++    A KE TSNSLA+IS+VA
Sbjct: 181 LTALSSKTPDLNSWLSAVQSYQQTCIDGFPVGKLKTDVKNAFNATKEFTSNSLALISEVA 240

Query: 241 TFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSGN 300
           +F ST +     G AT R L+  DG P+WM+  +RRMLK  A D K TPNV VAKDGSG 
Sbjct: 241 SFLSTLQTPGSPGPATTRHLLDTDGFPTWMSHEDRRMLK--ASDVKLTPNVTVAKDGSGK 300

Query: 301 FKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNSA 360
           FKTIN ALAAMPA Y GR+VIYVKEG+Y+ETV++TKKM NVT+YGDGSQKS+I+G KN  
Sbjct: 301 FKTINAALAAMPANYTGRFVIYVKEGVYDETVIVTKKMVNVTIYGDGSQKSIITGKKNFV 360

Query: 361 DGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDTL 420
           DGVR F+TASF ALG+G + K +GFRNTAGPEK QAVAAR+Q DRAIFVNCRFEG+QDTL
Sbjct: 361 DGVRTFETASFAALGEGFMGKAMGFRNTAGPEKHQAVAARIQSDRAIFVNCRFEGYQDTL 420

Query: 421 YAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETTA 480
           YAQ HRQFYRSC+I GTVD IFGDAAAIFQNCN +++KPLD Q N+VTAQGR+D  ETT 
Sbjct: 421 YAQTHRQFYRSCVIAGTVDFIFGDAAAIFQNCNLVVRKPLDNQQNIVTAQGRTDVRETTG 480

Query: 481 TVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFAL 540
            VLQNC+++ DK L  VK + +SYLGRP  E+SRTI MES+IEDIIHPDGWMPW+G+FAL
Sbjct: 481 FVLQNCRVMADKALVPVKEKIRSYLGRPWKEFSRTIFMESDIEDIIHPDGWMPWEGEFAL 540

Query: 541 KTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHVGLF 600
           KTLYYAE+NNKG G+KT ARVKWPG+KVIDK+EA KFT+  FL   W+ ++ V V    +
Sbjct: 541 KTLYYAEYNNKGAGSKTTARVKWPGYKVIDKQEANKFTVENFLPGSWLNATGVPVQKSFY 592

BLAST of CmoCh06G000100 vs. NCBI nr
Match: gi|702292027|ref|XP_010047427.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Eucalyptus grandis])

HSP 1 Score: 706.8 bits (1823), Expect = 3.2e-200
Identity = 375/609 (61.58%), Postives = 451/609 (74.06%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           M FQDFD +SERR+AER +K R RV IAVVS+ L +  VVA V + V        R G  
Sbjct: 1   MAFQDFDLLSERRQAERKQKMRKRVAIAVVSS-LAIAGVVAGVIIAV------VQRSG-- 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
            GSS +  +++   TS   Q+S +QKMI MIC+STDY+  C+ST  + V++  + + PKD
Sbjct: 61  -GSSSAANATQAEPTS---QISHSQKMIKMICSSTDYRDTCKSTFDKAVKSEKNLTQPKD 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
           L+K+AI A   EV  A  K     F +P+EK AFEDCK LLE+AVEEL  SVS L    +
Sbjct: 121 LLKVAIGATFDEVKEAFDKTKKFKFNSPQEKAAFEDCKQLLEDAVEELHSSVSNLG-DSI 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
           GK + KT PDLNNWLSA +SY  +CVDGFP GK KTEM+ TLK  KELTSNSLA+IS+V+
Sbjct: 181 GKFSSKT-PDLNNWLSAAISYQHSCVDGFPDGKLKTEMQKTLKIAKELTSNSLAIISEVS 240

Query: 241 TFFSTFEKSDGGGAATRRRL---------MAKDGLPSWMTSYERRMLKRDAEDDKPTPNV 300
           +F +TF+       ATR  L         + +DGLPSWM   ERR+LK      K TPNV
Sbjct: 241 SFLTTFQIP----GATRHLLEKEASGLPAVGEDGLPSWMNQEERRILK-GKRGVKLTPNV 300

Query: 301 VVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKS 360
           VVAKDGSG FKTI+EALAA+P KY GRYVIY+K GIY+E + +TKKM NVTMYGDGSQK+
Sbjct: 301 VVAKDGSGKFKTISEALAALPEKYTGRYVIYIKAGIYDEYITVTKKMVNVTMYGDGSQKT 360

Query: 361 MISGNKNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNC 420
           +++GNKN  DGVR FQTASF ALGDG +A+ +GFRNTAGPEK QAVA RVQ DRAIF+NC
Sbjct: 361 IVTGNKNFVDGVRTFQTASFAALGDGFMAQAMGFRNTAGPEKHQAVAIRVQSDRAIFLNC 420

Query: 421 RFEGFQDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQG 480
           RFEG+QDTLY Q HRQFYRSC+I GTVD IFGDA AI QNC  +++KPLD Q N+VTAQG
Sbjct: 421 RFEGYQDTLYVQTHRQFYRSCVIAGTVDFIFGDATAILQNCLIVVRKPLDNQQNIVTAQG 480

Query: 481 RSDRHETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGW 540
           R D HETT  VLQNC+I  DK L  VK++F++YLGRP  E+SRTI+MES IED I P G+
Sbjct: 481 RVDGHETTGIVLQNCRITADKKLIPVKSKFQTYLGRPWKEFSRTIIMESSIEDFIDPAGF 540

Query: 541 MPWDGDFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSS 600
           MPW+GDFALKTLYYAEFNNKGPGA  +ARVKWPG+K I+K+EAAKFT+GTFLD DWI+ +
Sbjct: 541 MPWEGDFALKTLYYAEFNNKGPGANINARVKWPGYKKINKQEAAKFTVGTFLDGDWIKGA 589

BLAST of CmoCh06G000100 vs. NCBI nr
Match: gi|802628667|ref|XP_012077133.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Jatropha curcas])

HSP 1 Score: 706.4 bits (1822), Expect = 4.1e-200
Identity = 366/604 (60.60%), Postives = 450/604 (74.50%), Query Frame = 1

Query: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQVSPPTKSTTRDGAG 60
           MVFQDFDQISERRR ER +KFR R+ IA ++  L+LV++   VF  +S   K+T R  + 
Sbjct: 1   MVFQDFDQISERRRLEREQKFRKRITIAAITIVLLLVIIGCGVFAIISE-NKNTKR--SK 60

Query: 61  SGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPKD 120
             S  SKGS+KD        V++  ++I  ICN TDYK  C S+L+  V+  P S++PK 
Sbjct: 61  ESSQESKGSAKD--------VAQVDRVIRRICNGTDYKDTCVSSLESEVKKDPSSANPKA 120

Query: 121 LIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKDL 180
           +++LAI A   E+   LKKAS+  F +P+EK AFEDCK+L+++A EEL  S++     D+
Sbjct: 121 ILRLAIEATDDEMGVVLKKASSFKFESPQEKAAFEDCKVLIKDAKEELKESLTHAG-VDI 180

Query: 181 GKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQVA 240
            KL E   P+LNN LSAVMSY  TC+DGFP GK K++ME T  A KELTSNSLAM+S + 
Sbjct: 181 KKLAENE-PELNNLLSAVMSYQQTCIDGFPDGKLKSDMEKTFNASKELTSNSLAMVSSLT 240

Query: 241 TFFSTFEKSDGGGA--ATRRRL--MAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKD 300
           +F S+F + +      AT+  L  M KD LPSW+++ +RR+LK D + DKPTPNV+VAKD
Sbjct: 241 SFLSSFGEINHSRRLLATKPNLSSMDKDELPSWISNEDRRILKAD-DSDKPTPNVIVAKD 300

Query: 301 GSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGN 360
           GSG FKTI+EAL AMP KY GRY+IYVK G+Y+E V + KKM N+TMYGDGSQKS+I+GN
Sbjct: 301 GSGKFKTISEALKAMPKKYKGRYIIYVKAGVYDEVVTVEKKMENITMYGDGSQKSIITGN 360

Query: 361 KNSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGF 420
           KN  DGV+ F+TASFVALG+G +AK IGFRNTAGPEK QAVAARVQ DR IF+NCRFEG+
Sbjct: 361 KNFKDGVQTFRTASFVALGNGFMAKAIGFRNTAGPEKHQAVAARVQADRTIFLNCRFEGY 420

Query: 421 QDTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRH 480
           QDTLY Q HRQ+YRSC+I GT+D IFGDAAAI QNC  +++KPL+ Q N+VTAQGR D H
Sbjct: 421 QDTLYVQTHRQYYRSCVIAGTIDFIFGDAAAILQNCQIVIRKPLENQQNIVTAQGRIDPH 480

Query: 481 ETTATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDG 540
           ETT  VLQNC+I PDK+L   KA+FKSYLGRP  EYSRTIVMES IED+IHPDGW+ W+G
Sbjct: 481 ETTGIVLQNCRIQPDKSLVPDKAKFKSYLGRPWKEYSRTIVMESTIEDLIHPDGWLAWEG 540

Query: 541 DFALKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVH 600
           +  LKTLYYAEFNNKGPGA T ARVKWPG+ VIDKEEA K+TI  F+  DWI      VH
Sbjct: 541 EKGLKTLYYAEFNNKGPGASTKARVKWPGYHVIDKEEANKYTIEPFIQGDWINDMGAPVH 590

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME45_ARATH1.3e-16450.57Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=P... [more]
PME13_ARATH8.7e-15848.80Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana GN=P... [more]
PME28_ARATH1.5e-12040.85Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=P... [more]
PME58_ARATH9.7e-11741.51Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=P... [more]
PME21_ARATH1.3e-11640.00Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0KX33_CUCSA5.7e-26577.33Pectinesterase OS=Cucumis sativus GN=Csa_5G630740 PE=4 SV=1[more]
A0A067KCM7_JATCU2.9e-20060.60Pectinesterase OS=Jatropha curcas GN=JCGZ_07552 PE=4 SV=1[more]
A0A061GJT0_THECC1.2e-19859.67Pectinesterase OS=Theobroma cacao GN=TCM_037445 PE=4 SV=1[more]
B9RR24_RICCO3.5e-19859.11Pectinesterase OS=Ricinus communis GN=RCOM_0708480 PE=4 SV=1[more]
U5G7D7_POPTR6.0e-19858.65Pectinesterase OS=Populus trichocarpa GN=POPTR_0006s13690g PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G33230.17.1e-16650.57 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G26450.14.9e-15948.80 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G27870.18.2e-12240.85 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G49180.15.5e-11841.51 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT3G05610.17.2e-11840.00 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|659121235|ref|XP_008460558.1|1.6e-26879.00PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo][more]
gi|449449216|ref|XP_004142361.1|8.2e-26577.33PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis sativus][more]
gi|1009142836|ref|XP_015888938.1|2.1e-20460.00PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Ziziphus jujuba][more]
gi|702292027|ref|XP_010047427.1|3.2e-20061.58PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Eucalyptus grand... [more]
gi|802628667|ref|XP_012077133.1|4.1e-20060.60PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR006501Pectinesterase_inhib_dom
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005618 cell wall
cellular_component GO:0005575 cellular_component
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G000100.1CmoCh06G000100.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 290..584
score: 3.0E
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 85..239
score: 7.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 87..235
score: 6.6
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 83..235
score: 4.6
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 81..239
score: 3.3
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 85..238
score: 1.44
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 288..588
score: 4.49E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 287..594
score: 3.1E
NoneNo IPR availableunknownCoilCoilcoord: 158..178
scor
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 85..600
score: 0.0coord: 22..56
score:
NoneNo IPR availablePANTHERPTHR31707:SF3PECTINESTERASE/PECTINESTERASE INHIBITOR 13-RELATEDcoord: 22..56
score: 0.0coord: 85..600
score:

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmoCh06G000100CmaCh06G000110Cucurbita maxima (Rimu)cmacmoB829
CmoCh06G000100Cp4.1LG08g13420Cucurbita pepo (Zucchini)cmocpeB794
CmoCh06G000100Carg01303Silver-seed gourdcarcmoB0227
The following gene(s) are paralogous to this gene:

None