CmoCh05G013370 (gene) Cucurbita moschata (Rifu)

NameCmoCh05G013370
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionGlucan endo-1,3-beta-glucosidase 8
LocationCmo_Chr05 : 10372338 .. 10374969 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAACTCCAACCAGCAGTCCATTTGCAGAGTCCTCTGGTTTCTTACTCCTTCCTTCTCGATTCCATCTTTATCAAAAACATTCCGATTCAAAGATTCGAACCAATCAGAGATCGAATCAGCAAAAAGAGGGGGAAAAAAAGAAGAACAATGTCGGAATCTGTGTTACGATTCCACAGAAATTGGATATTCATAGCTTTGATTTTGTTGTTGTTGACGGTTGTTCCGAATGGATTTAGTGTTGGAGTGAACTGGGGAACTATGGCTACTCACCAGCTTCCTCCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTTCGTAAGTTGAAGCTTTTTGAAGCTGAGGATCGGATTTTGGAAGCCCTAATTGGGACTGATATTGAGGTTATGTTGGCTATTCCTAATTCAATGTTGCAGGTTATGAGTGAAGATCCTGAGGCTGCAGCTCTTTGGGTGGATTCTAATGTTACTGCTTACACTTATCATGGTGGTGTTAAAATCAAGTTGAGTATTGATTTACTTTGGTTCTTGTTCTTCGTTTTTTTGTGCTCGTAAGGTGTTCGATGAAATTCCCACTTCATGTTTCTCTGTTTTGAATGCGTTCTTTTCAAGCCTGCCTTTGAAACTTCACATGAAATCTTTAGAAAGCGATTGAATTAGCCAAAAATAAGTTTAGCTCATCTTTTGTTTTAGAAAGCGATTGAATTCTCCTCTCTAGGCTTTCCTTTTCGAGTTTCTCCTCGAGGTTTTTAAAACGGGTATACTAGGGAGAGGTTTGCTCTGTTACCCTCTCCAACCGATGTGGGATCTCACAATAATCCACCCCCTTTAGGGCCCAGCATCCTCGCTGGCACTTGTTCCCCTCTCAAATCGATGTGGGATCTAATAATCCACCCATTGTCAGGGCTCACCATCCTTGCTGGCACACCGCTCAGTGTCTGGCTTTGATACTATTTGTTACAGCCTAAGTCCACCGCTAGCAGACATTGTTCTCTTTGAATTCTCCCTTTCGGGCTTTCTCTCAAAGTTTTTAAAACACATATGCTAGGGAGAGGTTTCCACACTCCTATAAAGAATGTTTTGTTCTTCTCTCCAACCGATGTGGGATCTCACTACAATCCACCCCGTTTGGGGTCTAGCGTCCTTTTCACACCATTATAAAGAATGTTTCGTTCCTCTCTCCAACCGATGTGGGTTCTCACAATTATCCACTCCCTTTGGGGCCTAACGTCCTTTCCACACTCTTATAAAGAATGTTTCGTTCCCCCCTCCAACTAATATGGAAACTCACAATAATCCACCCCTTCGGGGCCCAGCGTCTTCACTGGCGCTCATTTCTCTCTCCAATCGATGTGGGATCTCATAGCAACCCGAAAGAATTGAAATTTGTTAATGTATTTTTAGAAGTTTAAGAACCAGATTTTAGGAATGTTAAGTTAGCTTAACAAGCATATTGGTTCTTTCCATCTCCTATAAACTTCCATTGCAAACTGATTTTGCAGGTATGTTGCTGTAGGCAATGAGCCCTTTCTTAAGTCCTACAACGGCATCTATCTTCAGTCAACCTTACCTGCCCTGAGGAATATCCAGCAAGCCTTAAACGATGCAGGACTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATACTTACAATTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGATTTTCGGCCAGACGTCCGAGACTTAACAATCGAGATCCTACACTTCCTATCCATAAACAATGCACCATTCACAGTCAACATCTACCCCTTCCTCTCCCTATATGGCAATGACTACTTCCCCATCGACTTCGCCTTCTTTGATGGAACATACAAGTCCATCAAAGACGGAGATCTAACATACACAAACGTGTTCGATGCAAATTTCGACACTCTCGTTTCGGCCCTAACAAAAGCAGGATACCCCGACATGAAAATCATAGTAGGAGAAGTCGGCTGGCCAACCGACGGCGACAAACACGCCAATATCCAAAACGCAAAACGATTCAACCAGGGATTATTGAGGCATGCTCTAAGTGGGGAGGGAACCCCAGCAAGGGTAGGCATAATTGACATCTATCTCTTCAGCTTAATCGATGAAAATGCCAAGAGCATAGAGCCAGGGAGCTTCGAAAGGCATTGGGGAATATTCAAGTTCGACGGTAAGCCAAAATATGAGCTAGATCTGTCTGGTATAGAAGCCCAAAAGGGGCTGATAGCAGTAGAGGGAGTGAGATATATGGTTAAAAGATGGTGTGTTTTGAACCCAAATGTGAAGGATTGGGAAGGCTTGGCTTATAGTGTTGACTATGCTTGTAGCCTATCAGATTGCACTGCTTTGGAGTATGGATCTTCTTGCAATCAGCTAAGTGCAGAAGGGAATGCCTCTTATGCGTTTAATATGTACTATCAAATGAACAGCCAGAAGAGCTGGAACTGTGATTTTTCTGGTATGGCTGTGGTGACTCAAGAGGATCCTTCTTATGGCAGCTGCCAGTTCCCTGTTATGATTGATTATACCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATTTCTGGATCTTCTGGTTGCAGGTTTCATGGGCTTTCTGGTGTTTTTGTTTCTGGAATGA

mRNA sequence

CCAACTCCAACCAGCAGTCCATTTGCAGAGTCCTCTGGTTTCTTACTCCTTCCTTCTCGATTCCATCTTTATCAAAAACATTCCGATTCAAAGATTCGAACCAATCAGAGATCGAATCAGCAAAAAGAGGGGGAAAAAAAGAAGAACAATGTCGGAATCTGTGTTACGATTCCACAGAAATTGGATATTCATAGCTTTGATTTTGTTGTTGTTGACGGTTGTTCCGAATGGATTTAGTGTTGGAGTGAACTGGGGAACTATGGCTACTCACCAGCTTCCTCCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTTCGTAAGTTGAAGCTTTTTGAAGCTGAGGATCGGATTTTGGAAGCCCTAATTGGGACTGATATTGAGGTTATGTTGGCTATTCCTAATTCAATGTTGCAGGTTATGAGTGAAGATCCTGAGGCTGCAGCTCTTTGGGTGGATTCTAATGTTACTGCTTACACTTATCATGGTGGTGTTAAAATCAAGTATGTTGCTGTAGGCAATGAGCCCTTTCTTAAGTCCTACAACGGCATCTATCTTCAGTCAACCTTACCTGCCCTGAGGAATATCCAGCAAGCCTTAAACGATGCAGGACTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATACTTACAATTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGATTTTCGGCCAGACGTCCGAGACTTAACAATCGAGATCCTACACTTCCTATCCATAAACAATGCACCATTCACAGTCAACATCTACCCCTTCCTCTCCCTATATGGCAATGACTACTTCCCCATCGACTTCGCCTTCTTTGATGGAACATACAAGTCCATCAAAGACGGAGATCTAACATACACAAACGTGTTCGATGCAAATTTCGACACTCTCGTTTCGGCCCTAACAAAAGCAGGATACCCCGACATGAAAATCATAGTAGGAGAAGTCGGCTGGCCAACCGACGGCGACAAACACGCCAATATCCAAAACGCAAAACGATTCAACCAGGGATTATTGAGGCATGCTCTAAGTGGGGAGGGAACCCCAGCAAGGGTAGGCATAATTGACATCTATCTCTTCAGCTTAATCGATGAAAATGCCAAGAGCATAGAGCCAGGGAGCTTCGAAAGGCATTGGGGAATATTCAAGTTCGACGGTAAGCCAAAATATGAGCTAGATCTGTCTGGTATAGAAGCCCAAAAGGGGCTGATAGCAGTAGAGGGAGTGAGATATATGGTTAAAAGATGGTGTGTTTTGAACCCAAATGTGAAGGATTGGGAAGGCTTGGCTTATAGTGTTGACTATGCTTGTAGCCTATCAGATTGCACTGCTTTGGAGTATGGATCTTCTTGCAATCAGCTAAGTGCAGAAGGGAATGCCTCTTATGCGTTTAATATGTACTATCAAATGAACAGCCAGAAGAGCTGGAACTGTGATTTTTCTGGTATGGCTGTGGTGACTCAAGAGGATCCTTCTTATGGCAGCTGCCAGTTCCCTGTTATGATTGATTATACCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATTTCTGGATCTTCTGGTTGCAGGTTTCATGGGCTTTCTGGTGTTTTTGTTTCTGGAATGA

Coding sequence (CDS)

ATGTCGGAATCTGTGTTACGATTCCACAGAAATTGGATATTCATAGCTTTGATTTTGTTGTTGTTGACGGTTGTTCCGAATGGATTTAGTGTTGGAGTGAACTGGGGAACTATGGCTACTCACCAGCTTCCTCCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTTCGTAAGTTGAAGCTTTTTGAAGCTGAGGATCGGATTTTGGAAGCCCTAATTGGGACTGATATTGAGGTTATGTTGGCTATTCCTAATTCAATGTTGCAGGTTATGAGTGAAGATCCTGAGGCTGCAGCTCTTTGGGTGGATTCTAATGTTACTGCTTACACTTATCATGGTGGTGTTAAAATCAAGTATGTTGCTGTAGGCAATGAGCCCTTTCTTAAGTCCTACAACGGCATCTATCTTCAGTCAACCTTACCTGCCCTGAGGAATATCCAGCAAGCCTTAAACGATGCAGGACTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATACTTACAATTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGATTTTCGGCCAGACGTCCGAGACTTAACAATCGAGATCCTACACTTCCTATCCATAAACAATGCACCATTCACAGTCAACATCTACCCCTTCCTCTCCCTATATGGCAATGACTACTTCCCCATCGACTTCGCCTTCTTTGATGGAACATACAAGTCCATCAAAGACGGAGATCTAACATACACAAACGTGTTCGATGCAAATTTCGACACTCTCGTTTCGGCCCTAACAAAAGCAGGATACCCCGACATGAAAATCATAGTAGGAGAAGTCGGCTGGCCAACCGACGGCGACAAACACGCCAATATCCAAAACGCAAAACGATTCAACCAGGGATTATTGAGGCATGCTCTAAGTGGGGAGGGAACCCCAGCAAGGGTAGGCATAATTGACATCTATCTCTTCAGCTTAATCGATGAAAATGCCAAGAGCATAGAGCCAGGGAGCTTCGAAAGGCATTGGGGAATATTCAAGTTCGACGGTAAGCCAAAATATGAGCTAGATCTGTCTGGTATAGAAGCCCAAAAGGGGCTGATAGCAGTAGAGGGAGTGAGATATATGGTTAAAAGATGGTGTGTTTTGAACCCAAATGTGAAGGATTGGGAAGGCTTGGCTTATAGTGTTGACTATGCTTGTAGCCTATCAGATTGCACTGCTTTGGAGTATGGATCTTCTTGCAATCAGCTAAGTGCAGAAGGGAATGCCTCTTATGCGTTTAATATGTACTATCAAATGAACAGCCAGAAGAGCTGGAACTGTGATTTTTCTGGTATGGCTGTGGTGACTCAAGAGGATCCTTCTTATGGCAGCTGCCAGTTCCCTGTTATGATTGATTATACCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATTTCTGGATCTTCTGGTTGCAGGTTTCATGGGCTTTCTGGTGTTTTTGTTTCTGGAATGA
BLAST of CmoCh05G013370 vs. Swiss-Prot
Match: E138_ARATH (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana GN=At1g64760 PE=1 SV=2)

HSP 1 Score: 535.8 bits (1379), Expect = 5.0e-151
Identity = 268/464 (57.76%), Postives = 335/464 (72.20%), Query Frame = 1

Query: 22  LTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVM 81
           L ++ NG  +GVNWGTMATH+LPP+ VV+ML++N   K+KLF+A++  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTL 141
           +AIPN  L+VM+    A   WV  NVT Y + GGV I +VAVGNEPFLKSYNG ++  T 
Sbjct: 78  VAIPNDQLKVMTSYDRAKD-WVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDLTIEILHFLS 201
           PAL NIQ ALN+AGLG+ VKAT+P NAD Y+SP SNPVPSAG FRPD+     +I+ FL 
Sbjct: 138 PALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLG 197

Query: 202 INNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNVFDANFDTLVSALTK 261
            NNAP T+NIYPFLSLYGND FP+++AFFDG  + I D  + YTNVFDANFDTLVS+L  
Sbjct: 198 KNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKA 257

Query: 262 AGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLI 321
            G+ DM IIVGEVGWPT+GDKHAN  +A RF  GLL    + +GTP R   I++YLF L+
Sbjct: 258 VGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLL 317

Query: 322 DENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVKRWCVLNPNV 381
           DE+AKSI PG FERHWGIFKFDG+PK+ +DLSG    K LI  + V Y+  +WC  NP  
Sbjct: 318 DEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEA 377

Query: 382 KDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNSQKSWNCDFSGM 441
           KD   LA ++DYAC+ SDCTAL YGSSCN L A GNASYAFNM++Q+ +Q    C F G+
Sbjct: 378 KDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGL 437

Query: 442 AVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLLVAGFM 486
           A +T ++ S G C FP+ I    SS+SS S SS  L +L+  F+
Sbjct: 438 ATITTQNISQGQCNFPIQI--VASSASSFSCSSYSLVVLIVWFL 475

BLAST of CmoCh05G013370 vs. Swiss-Prot
Match: E136_ARATH (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana GN=At5g58090 PE=1 SV=2)

HSP 1 Score: 489.2 bits (1258), Expect = 5.3e-137
Identity = 249/480 (51.88%), Postives = 324/480 (67.50%), Query Frame = 1

Query: 12  WIFIALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILE 71
           W  + L+L +  +     S+G NWGT A+H LPP+ VV+ML+ENG +K+KLF+AE   L 
Sbjct: 3   WGSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLR 62

Query: 72  ALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKS 131
           AL  + IEVM+ IPN ML  ++   +AA  WV  NV+ +     V I+YVAVGNEPFL +
Sbjct: 63  ALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLST 122

Query: 132 YNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRD 191
           YNG YL +T PALRNIQ A+  AGL ++VK T P NAD Y+S  S   PS GDFR ++RD
Sbjct: 123 YNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDS--STTFPSGGDFRANIRD 182

Query: 192 LTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNVFDAN 251
           L I I+ FLS N  PFTVNIYP++SLY N  FP+D+AFFDG  + + DG   Y N+FDAN
Sbjct: 183 LMITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDAN 242

Query: 252 FDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVG 311
           +DTLV AL K G+ +M II+GE+GWPTDGD +AN+  AK+FNQG + H   G+GTP R G
Sbjct: 243 YDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPG 302

Query: 312 IIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMV 371
            ID YLFSLIDE+AKS++PG FERHWGIF FDG PKY L+L G      LI  +GVRY+ 
Sbjct: 303 PIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLE 362

Query: 372 KRWCVLNPNVK-DWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNS 431
           ++WCV+ PNV+ D   +A +V YACSL DCT+L  G+SC  L  + N SYAFN YYQ+  
Sbjct: 363 RKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQD 422

Query: 432 QKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLLVAGFMGFLVF 491
           Q    C F  ++ VT+ DPS G+C+FP+MI+  +    ++     F  LL+   +   +F
Sbjct: 423 QLDTACKFPNISEVTKTDPSTGTCRFPIMIEPYY--GGAAREHGFFFPLLMVAAIAVSIF 477

BLAST of CmoCh05G013370 vs. Swiss-Prot
Match: E135_ARATH (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana GN=At4g31140 PE=1 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.7e-135
Identity = 239/459 (52.07%), Postives = 320/459 (69.72%), Query Frame = 1

Query: 13  IFIALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEA 72
           +F  + LL  +++     +GVNWG+ A H LPP  VV++L+ENG +K+KLFEA+  IL+A
Sbjct: 9   VFFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKA 68

Query: 73  LIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSY 132
           L  T I+VM+ IPN +L  ++    AA  WV  NV+A+    GV I+YVAVGNEPFLK++
Sbjct: 69  LSRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAF 128

Query: 133 NGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDL 192
           NG +   TLPAL+NIQ A+  AGL ++VK T+P NAD Y S  ++ +PS GDFRP++RDL
Sbjct: 129 NGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQS--ASNLPSDGDFRPEIRDL 188

Query: 193 TIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNVFDANF 252
            + I+ FLS N APFT+NIYPF+SLY +  FP++FAFFDGT   I D    Y NV DAN+
Sbjct: 189 MLNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANY 248

Query: 253 DTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGI 312
           DTLV +L K G+ ++ IIVGEVGWPTDGDK+AN+  A+R+NQG +    + +GTP R G 
Sbjct: 249 DTLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGA 308

Query: 313 IDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVK 372
           +D YLF LIDE+AKSI+PG+FERHWGIF  DG+PKY+L L    +  GLI  + V Y+ K
Sbjct: 309 MDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLG---SGNGLIPAKDVHYLAK 368

Query: 373 RWCVLNPNVKDWE-GLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNSQ 432
           +WC+L PN    +  L  SV YAC  +DCT+L YGSSC  L+   N SYAFN YYQ+++Q
Sbjct: 369 KWCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQ 428

Query: 433 KSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSS 471
               C F G+++V+  DPS GSC+F +MI    +S +S+
Sbjct: 429 LDSACKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASA 462

BLAST of CmoCh05G013370 vs. Swiss-Prot
Match: E139_ARATH (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana GN=At5g58480 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.2e-96
Identity = 193/474 (40.72%), Postives = 289/474 (60.97%), Query Frame = 1

Query: 18  ILLLLTVVPNGFS--------VGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRI 77
           + LLL  V  G S        VG+NWGT A+H LPP KVV++L+ NG  K+KLF+A+ ++
Sbjct: 5   LFLLLLAVTAGLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKV 64

Query: 78  LEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGG--VKIKYVAVGNEP 137
           L AL G++I V + I NSML+ ++   + A  WV  NVT Y ++GG  V+I+YVAVG EP
Sbjct: 65  LRALSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAVGEEP 124

Query: 138 FLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRP 197
           FL+SY   Y    + A  NIQ AL  A L ++VK  +P + D++ S      PS+G FR 
Sbjct: 125 FLQSYGNQYKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLSESGR--PSSGHFRA 184

Query: 198 DVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNV 257
           D+    IE+L FL+ +++PF V I PFLS + N    +DF+ F  T K+ KDG  TY N 
Sbjct: 185 DLNKTMIELLSFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNS 244

Query: 258 FDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTP 317
           FD ++DTLVSAL   G+ ++ I+V ++GWPTDG ++A    A+ F +GL+ H      + 
Sbjct: 245 FDLSYDTLVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTASL 304

Query: 318 ARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGV 377
            R   ++ Y+ SL+DE+ +++  G+FERHWG+F FDG+ KY    +     K  +  + V
Sbjct: 305 PRPP-VETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFN----HKNQVNAQNV 364

Query: 378 RYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQ 437
           +Y+  +WCV+N N KD    +     AC+++DCT++  G SC+ +   GN SYAFN  YQ
Sbjct: 365 QYLPPKWCVVNNN-KDLSNASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQ 424

Query: 438 MNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLLV 482
            N   + +C+F G+ ++T  DPS  +C+F + +D + SSS + +   S+  LL+
Sbjct: 425 QNDHSAESCNFGGLGLITTVDPSEDNCRFSIQLDTSHSSSQTPNFFQSWPLLLL 469

BLAST of CmoCh05G013370 vs. Swiss-Prot
Match: E137_ARATH (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 240.4 bits (612), Expect = 4.3e-62
Identity = 162/463 (34.99%), Postives = 249/463 (53.78%), Query Frame = 1

Query: 13  IFIALILLLLTVVPNGFS---VGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRI 72
           I I  +L+ L+  P+  +   +GVN+G +A +  PP + VK+LQ    +K++L+ A+  I
Sbjct: 5   ISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAI 64

Query: 73  LEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFL 132
           ++AL GT + +++   N  +  ++ DP AA  W++SNV  +  +   KI  + VGNE  L
Sbjct: 65  IKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPF--YPASKIMLITVGNE-IL 124

Query: 133 KSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDV 192
            S +   +   LPA++N+Q+AL    LG K+K +   +     S D    PS+G F    
Sbjct: 125 MSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDP---PSSGSFAAGY 184

Query: 193 RDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAF--FDGTYKSI--KDGDLTYT 252
           +     IL FLS   +PF +N YPF + Y +D  P   AF  F+     +  K G + YT
Sbjct: 185 QTGLKGILQFLSDTGSPFAINPYPFFA-YQSDPRPETLAFCLFEPNAGRVDSKTG-IKYT 244

Query: 253 NVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKH---ANIQNAKRFNQGLLRHALS 312
           N+FDA  D + SAL   G+  ++I+V E GW + GD +   A++ NAK +N  L+ H  S
Sbjct: 245 NMFDAQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRS 304

Query: 313 GEGTPARVG-IIDIYLFSLIDENAKSIEPG-SFERHWGIFKFDGKPKYELDL----SGIE 372
             GTP   G  +D Y+F+L DEN K   PG S ER +G+FK D    Y++ L    S  +
Sbjct: 305 MVGTPLMPGKPVDTYIFALYDENLK---PGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQ 364

Query: 373 AQKGLIAVEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLS-DCTALEYGSSC-NQLSA 432
              G +   G       WCV      + E L  S+D+AC    DC A++ G +C    + 
Sbjct: 365 TPSGKVTSSG-------WCVPKKGATN-EELQASLDWACGHGIDCGAIQPGGACFEPNNV 424

Query: 433 EGNASYAFNMYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFP 458
             +A+YA NMY+Q + ++  +CDFS  A VT ++PSY +C +P
Sbjct: 425 VSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of CmoCh05G013370 vs. TrEMBL
Match: A0A0A0LHZ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G005360 PE=3 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 3.5e-244
Identity = 418/474 (88.19%), Postives = 442/474 (93.25%), Query Frame = 1

Query: 24  VVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVMLA 83
           +VPNG SVGVNWGTMATHQLPPEKVVKMLQENGF KLKLFEA+DRILEALIG+DIEVMLA
Sbjct: 2   IVPNGLSVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLA 61

Query: 84  IPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTLPA 143
           IPNSML +MS+DP AAA WVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNG YL  TLPA
Sbjct: 62  IPNSMLFLMSQDPHAAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLPLTLPA 121

Query: 144 LRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDLTIEILHFLSIN 203
           LRNIQQ+LNDAGLGSKVKATIPFNAD YNSPDSNPVPSAG+FRPDVRDLTIEILH+LSIN
Sbjct: 122 LRNIQQSLNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSIN 181

Query: 204 NAPFTVNIYPFLSLYGNDYFPIDFAFFDGT-YKSIKDGDLTYTNVFDANFDTLVSALTKA 263
           NAPFTVNIYPFLSLYGNDYFPIDFAFFDGT Y+ IKD DLTYTNVFDANFDTLVSAL KA
Sbjct: 182 NAPFTVNIYPFLSLYGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKA 241

Query: 264 GYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLID 323
           GYPDMKIIVGEVGWPTDGDKHAN+QNAKRFNQGLLRHALSG+GTPARVGIID+YLFSLID
Sbjct: 242 GYPDMKIIVGEVGWPTDGDKHANVQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLID 301

Query: 324 ENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVKRWCVLNPNVK 383
           ENAKSIEPGSFERHWGIF+FDGKPKYELDL+G E +KGLI VEGVRYM KRWCVLNPNV 
Sbjct: 302 ENAKSIEPGSFERHWGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVN 361

Query: 384 DWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNSQKSWNCDFSGMA 443
           DWEGLA SVDYACSLSDCTALEYGSSCNQLSA+GNASYAFNMYYQ+NSQKSWNCDF G+A
Sbjct: 362 DWEGLADSVDYACSLSDCTALEYGSSCNQLSAQGNASYAFNMYYQVNSQKSWNCDFDGLA 421

Query: 444 VVTQEDPSYGSCQFPVMIDYTFSSSSSSS--SSSSFLDLLVAGFMGFLVFLFLE 495
           VVTQ+DPSYG+CQFP+MIDYT SSSSSSS      F DLLVA FMG +VF  ++
Sbjct: 422 VVTQQDPSYGNCQFPIMIDYTSSSSSSSSFLVYERFSDLLVAVFMGIVVFFIVQ 475

BLAST of CmoCh05G013370 vs. TrEMBL
Match: M5W8Z4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017632mg PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 1.7e-198
Identity = 339/477 (71.07%), Postives = 400/477 (83.86%), Query Frame = 1

Query: 15  IALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALI 74
           I LIL+L+ +   G S GVNWGTMATHQLPPEKVVKML++NGF KLKLFEAE++IL AL+
Sbjct: 10  IVLILVLMNMAQKGTSFGVNWGTMATHQLPPEKVVKMLKDNGFNKLKLFEAEEKILAALM 69

Query: 75  GTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNG 134
           GT IEVMLA+PN MLQ MS+DP AAA WVD+NVT+Y  +GGV IKYV+VGNEPFLK+YNG
Sbjct: 70  GTQIEVMLAVPNVMLQEMSQDPVAAATWVDANVTSYCSNGGVNIKYVSVGNEPFLKTYNG 129

Query: 135 IYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDLTI 194
            YL++TLPAL+NIQ+ALN+AGLGS+VKAT+PFNAD Y SPDS+PVPS GDFRP++RD  I
Sbjct: 130 TYLKTTLPALKNIQEALNNAGLGSQVKATVPFNADIYFSPDSDPVPSTGDFRPEIRDSLI 189

Query: 195 EILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNVFDANFDT 254
           EI+ +L  N+APFTVNIYPFLSLYGN YFP  FAFFDGT K +KDGDL YTNVFDANFDT
Sbjct: 190 EIIQYLHTNDAPFTVNIYPFLSLYGNTYFPFGFAFFDGTTKPMKDGDLLYTNVFDANFDT 249

Query: 255 LVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIID 314
           LV +LTKAG+P+MKI+VGEVGWPTDGD +ANI+NA+RFNQG+++H+LSG GTPAR G ID
Sbjct: 250 LVWSLTKAGFPEMKIMVGEVGWPTDGDINANIRNARRFNQGMIQHSLSGNGTPARKGKID 309

Query: 315 IYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVKRW 374
           +YLFSLIDE+AKSI PG+FERHWG+F++DGKPKY LDL+G    KGL AVE V YM+KRW
Sbjct: 310 VYLFSLIDEDAKSIAPGNFERHWGLFEYDGKPKYNLDLTGSLENKGLAAVEDVDYMLKRW 369

Query: 375 CVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNSQKSW 434
           CVL+ + KD E L  S+DYAC+LSDCTAL YG SCN LS  GNASYAFNMYYQ+NSQKSW
Sbjct: 370 CVLDKDAKDLEVLVKSIDYACTLSDCTALGYGCSCNNLSLRGNASYAFNMYYQVNSQKSW 429

Query: 435 NCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLLVAGFMGFLVFL 492
            CDFSG+AVVT E+PS G CQFPVMI Y   S   SS  +  +  +V G++ +LV L
Sbjct: 430 TCDFSGLAVVTDENPSVGDCQFPVMISYGGPSVLPSSGLAHMVMKIVGGYLLYLVLL 486

BLAST of CmoCh05G013370 vs. TrEMBL
Match: A0A068U4D9_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00041929001 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.3e-195
Identity = 330/464 (71.12%), Postives = 393/464 (84.70%), Query Frame = 1

Query: 30  SVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVMLAIPNSML 89
           S+GVNWGTMA+HQLPPE VV+M+ +NGF K+KLFEA+++IL ALIGTD+EVML IPN+ML
Sbjct: 7   SLGVNWGTMASHQLPPENVVQMMLDNGFDKVKLFEADEKILGALIGTDLEVMLGIPNNML 66

Query: 90  QVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTLPALRNIQQ 149
           + +S+DPEAAA WVD+NVTAY + GGV I+YVAVGNEPFLKSYN  +L  TL AL+NIQ+
Sbjct: 67  REISQDPEAAASWVDANVTAYCFKGGVNIRYVAVGNEPFLKSYNNSFLPYTLSALKNIQE 126

Query: 150 ALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDLTIEILHFLSINNAPFTV 209
           A+N AGLG+ VKATIPFNAD YNSP+SNPVPSAGDFRP++RDLT++I+ +L  N+APF V
Sbjct: 127 AINHAGLGTVVKATIPFNADVYNSPESNPVPSAGDFRPEIRDLTVQIIQYLHSNDAPFVV 186

Query: 210 NIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNVFDANFDTLVSALTKAGYPDMKI 269
           NIYPFLSLYGN YFP+D+AFFDG+ K IKDGD  YTNVFDANFDTLVS+L++AGYP+MKI
Sbjct: 187 NIYPFLSLYGNIYFPLDYAFFDGSNKPIKDGDYLYTNVFDANFDTLVSSLSRAGYPEMKI 246

Query: 270 IVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIE 329
           I+GEVGWPTDGDK+ANIQNAKRFNQGL++H   G GTPAR G ID+YLFSLIDENAKSIE
Sbjct: 247 IIGEVGWPTDGDKNANIQNAKRFNQGLIQHVSGGVGTPARNGSIDVYLFSLIDENAKSIE 306

Query: 330 PGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVKRWCVLNPNVKDWEGLAY 389
           PGSFERHWG+F+FDGKPKYELDLSG   +KGL+AVEGVRYM++RWC+L+P  K+ E L  
Sbjct: 307 PGSFERHWGLFEFDGKPKYELDLSGSREEKGLVAVEGVRYMLRRWCILDPRAKELEELPK 366

Query: 390 SVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNSQKSWNCDFSGMAVVTQEDP 449
           ++DYACSLSDCT+L YGSSCN LSA+GNASYAFNMYYQ  +Q  W+CDFSG+AVVT EDP
Sbjct: 367 NIDYACSLSDCTSLGYGSSCNHLSAKGNASYAFNMYYQFKNQNGWDCDFSGLAVVTDEDP 426

Query: 450 SYGSCQFPVMIDYTFSSSSSSSSSSSFLDLLVAGFMGFLVFLFL 494
           S   CQFPVMI       S      + L++L+A   G +VFL L
Sbjct: 427 SDDECQFPVMI---ADGHSMVLLHKTLLNILLAVLEGCVVFLLL 467

BLAST of CmoCh05G013370 vs. TrEMBL
Match: A0A072USM3_MEDTR (Glucan endo-1,3-beta-glucosidase-like protein OS=Medicago truncatula GN=MTR_4g112025 PE=3 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 1.5e-194
Identity = 334/492 (67.89%), Postives = 396/492 (80.49%), Query Frame = 1

Query: 1   MSESVLRFHRNWIFIALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60
           M++     H     + +I+  L++   G+ VGVNWGTMATHQLPP+ VVKMLQENGF KL
Sbjct: 1   MAQENATHHYLMTLLFIIIFFLSMSSCGYGVGVNWGTMATHQLPPKNVVKMLQENGFDKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120
           KLF+A++ ++ AL+GTDIEVMLAIPN+ML+ +S+DP+ A  WV  NVT+Y Y GG+ IKY
Sbjct: 61  KLFDADEWVMAALLGTDIEVMLAIPNNMLEEISKDPKVADSWVYENVTSYLYPGGLNIKY 120

Query: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVP 180
           +AVGNEPFLK YNG YL  TLPAL+NIQ +LN+AGLGS +KAT+PFNAD Y SPDSN VP
Sbjct: 121 IAVGNEPFLKEYNGTYLMYTLPALKNIQTSLNNAGLGSSIKATVPFNADAYYSPDSNLVP 180

Query: 181 SAGDFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDG 240
           SAGDFRP+VRDLTIEI+++L  NNAPFTVNIYPFLSLYGND+FP DFAFFDG  K + DG
Sbjct: 181 SAGDFRPEVRDLTIEIINYLYTNNAPFTVNIYPFLSLYGNDHFPFDFAFFDGNNKPLVDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           +L YTNVFDAN DTL+ AL KAGYPDM +I+GEVGWPTDGDK+ANIQNAKRFN GLL+H 
Sbjct: 241 NLLYTNVFDANLDTLLYALEKAGYPDMHVIIGEVGWPTDGDKNANIQNAKRFNNGLLKHC 300

Query: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKG 360
           LSG GTP R GIIDIYLFSLIDENAKSI PG+FERHWGIF+FDGKPKYELDL G +  +G
Sbjct: 301 LSGNGTPKRKGIIDIYLFSLIDENAKSIAPGNFERHWGIFEFDGKPKYELDLKGFQGDEG 360

Query: 361 LIAVEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASY 420
           L+AVEGV+YM K+WC+L+P++ D   LA S+DYACSLSDCTAL YGSSCN LS EGNASY
Sbjct: 361 LVAVEGVKYMEKQWCILDPDISDLHYLAGSIDYACSLSDCTALGYGSSCNGLSLEGNASY 420

Query: 421 AFNMYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLL 480
           AFNMYYQ+++QK W+CDFSG+A+++ +DPS   CQFPVMI    S +S       F  L 
Sbjct: 421 AFNMYYQVHNQKDWDCDFSGLAILSDQDPSEKGCQFPVMI----SHASCLGLHKGF--LA 480

Query: 481 VAGFMGFLVFLF 493
             G   FLVF F
Sbjct: 481 FGGVYIFLVFFF 486

BLAST of CmoCh05G013370 vs. TrEMBL
Match: W9QRM7_9ROSA (Glucan endo-1,3-beta-glucosidase 8 OS=Morus notabilis GN=L484_020860 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 1.9e-194
Identity = 334/474 (70.46%), Postives = 396/474 (83.54%), Query Frame = 1

Query: 19  LLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDI 78
           L+LLT+V +  SVGVNWGT+A+H+LPP KVVKM++ENGF K+KLFEA++ ILEAL+GT+I
Sbjct: 24  LVLLTMVCSCSSVGVNWGTIASHKLPPGKVVKMMKENGFTKVKLFEADEWILEALMGTNI 83

Query: 79  EVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQ 138
           EVM+ IPN MLQ +SE+P+ AA WV++NVT Y Y GGV IKYVAVGNEPFL++YNG Y  
Sbjct: 84  EVMVGIPNIMLQEISENPKVAADWVEANVTDYLYTGGVHIKYVAVGNEPFLEAYNGSYTH 143

Query: 139 STLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDLTIEILH 198
           +T PAL+NIQQALND G+GSKVKAT+PFNAD Y SPDSNPVPSAGDFRP VRD+TIEI+ 
Sbjct: 144 TTFPALKNIQQALNDQGVGSKVKATVPFNADVYFSPDSNPVPSAGDFRPQVRDITIEIIQ 203

Query: 199 FLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGT-YKSIKDGDLTYTNVFDANFDTLVS 258
           +L  N+APFTVNIYPFLSLY NDYFP+DFAFFDGT YK ++DGD  Y NVFDANFDTLV 
Sbjct: 204 YLHANDAPFTVNIYPFLSLYSNDYFPLDFAFFDGTNYKPVRDGDRVYNNVFDANFDTLVE 263

Query: 259 ALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYL 318
           A+ KAG+PDMKIIVGEVGWPTDGD+HAN++NAKRFNQG+L+HALSG GTPA+ G I++YL
Sbjct: 264 AINKAGFPDMKIIVGEVGWPTDGDRHANVKNAKRFNQGMLQHALSGNGTPAQKGKIEVYL 323

Query: 319 FSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVKRWCVL 378
           FSLIDE+AKSIEPG+FERHWGI+++DGKPKYELDLSG +  KGL+AV+GV YM+KRWCVL
Sbjct: 324 FSLIDEDAKSIEPGNFERHWGIYEYDGKPKYELDLSGSKRNKGLVAVDGVEYMLKRWCVL 383

Query: 379 NPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNSQKSWNCD 438
           NP+ +  + L  S+DYACSLSDCTAL +GSSCN LSA+GNASYAFNMYYQ+N Q S +CD
Sbjct: 384 NPDAEYLDDLPDSIDYACSLSDCTALGFGSSCNNLSAQGNASYAFNMYYQVNGQNSRSCD 443

Query: 439 FSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLLVAGFMGFLVFL 492
           FSG+AVV+ +DPS G CQFPVMI        S      F  L   G   FLV L
Sbjct: 444 FSGLAVVSDKDPSVGDCQFPVMI--------SRGGQMGFSLLRFFGVFVFLVIL 489

BLAST of CmoCh05G013370 vs. TAIR10
Match: AT3G24330.1 (AT3G24330.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 627.1 bits (1616), Expect = 9.3e-180
Identity = 307/490 (62.65%), Postives = 385/490 (78.57%), Query Frame = 1

Query: 8   FHRNWIFIALILLLLTVV---PNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFE 67
           F+ + I +   L++  V     N  +VGVNWG MA+HQLPPEKVVKML +N F KLKLFE
Sbjct: 13  FYNHQIILCYFLIISQVSIASSNTSNVGVNWGIMASHQLPPEKVVKMLMDNSFTKLKLFE 72

Query: 68  AEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVG 127
           A+  IL+ALIG+DIEVM+ IPN  L+ M++D   AA WV+ NVTAY+Y+GGV IKY+AVG
Sbjct: 73  ADQNILDALIGSDIEVMIGIPNRFLKEMAQDTSVAASWVEENVTAYSYNGGVNIKYIAVG 132

Query: 128 NEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGD 187
           NEPFL++YNG Y++ TLPAL NIQ+AL +A L   VK T+PFNAD Y SP++NPVPSAGD
Sbjct: 133 NEPFLQTYNGTYVEFTLPALINIQRALEEADL-KNVKVTVPFNADIYFSPEANPVPSAGD 192

Query: 188 FRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTY 247
           FRP++RD TIEI++FL  +++PFTVNIYPFLSLYGN YFP+DFAFFDGT KS++DG+L Y
Sbjct: 193 FRPELRDATIEIINFLYSHDSPFTVNIYPFLSLYGNAYFPLDFAFFDGTNKSLRDGNLVY 252

Query: 248 TNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGE 307
           TNVFDAN DTL+ A+ +  +  MKIIVGEVGWPTDGDK+AN+++AKRFNQG+++HA+SG 
Sbjct: 253 TNVFDANLDTLICAMERYSFLGMKIIVGEVGWPTDGDKNANVKSAKRFNQGMVKHAMSGN 312

Query: 308 GTPARVGII-DIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIA 367
           GTPAR G+I D+YLFSL+DE+AKSI PG+FERHWGIF+FDG+PKYELDLSG    K L+ 
Sbjct: 313 GTPARKGVIMDVYLFSLVDEDAKSIAPGTFERHWGIFEFDGRPKYELDLSGKGNDKPLVP 372

Query: 368 VEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFN 427
           VE V+Y+ K WC+L+PN  + + L  ++DYACSLSDCTAL YGSSCN L+A GN SYAFN
Sbjct: 373 VEDVKYLPKTWCILDPNAYNLDDLPDNIDYACSLSDCTALGYGSSCNHLTATGNVSYAFN 432

Query: 428 MYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLLVAG 487
           MYYQM+ QK+W+CDF G+ ++T EDPS   C+FPVMID T  S+     SS  L  + A 
Sbjct: 433 MYYQMHDQKTWDCDFLGLGLITDEDPSDELCEFPVMID-TGDSTRLQPGSSRVLTRVAAA 492

Query: 488 FMGFLVFLFL 494
            +  LV   L
Sbjct: 493 VLVMLVLPIL 500

BLAST of CmoCh05G013370 vs. TAIR10
Match: AT2G19440.1 (AT2G19440.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 542.3 bits (1396), Expect = 3.0e-154
Identity = 269/453 (59.38%), Postives = 330/453 (72.85%), Query Frame = 1

Query: 31  VGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVMLAIPNSMLQ 90
           +GVNWGTMATH+LPP+KVV+ML++N   K+KLF+A++  + AL G+ +EVM+AIPN  L+
Sbjct: 21  LGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPNDQLK 80

Query: 91  VMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTLPALRNIQQA 150
           VM     A   WV  NVT Y ++GGV I +VAVGNEPFLKSYNG ++  T PAL+NIQ A
Sbjct: 81  VMGSYDRAKD-WVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQNA 140

Query: 151 LNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDLTIEILHFLSINNAPFTVN 210
           LN+AGLGS VKAT+P NAD Y+SP SNPVPSAG FRPD+     +I+ FL  N+AP T+N
Sbjct: 141 LNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSAPITIN 200

Query: 211 IYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNVFDANFDTLVSALTKAGYPDMKII 270
           IYPFLSLYGND FP+++AFFDG  K + D  + YTNVFDANFDTLVSAL   G+ DM II
Sbjct: 201 IYPFLSLYGNDDFPLNYAFFDGA-KPVDDNGIAYTNVFDANFDTLVSALKAVGHGDMPII 260

Query: 271 VGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEP 330
           VGEVGWPT+GDKHAN  +A RF  GLL       GTP R   I++YLF L+DE+AKSI P
Sbjct: 261 VGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTYIEVYLFGLLDEDAKSIAP 320

Query: 331 GSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVKRWCVLNPNVKDWEGLAYS 390
           G FERHWGIFKFDG+PK+ +DLSG    K LI  E V Y  K+WC+ N   KD   LA +
Sbjct: 321 GEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKDLTKLAAN 380

Query: 391 VDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNSQKSWNCDFSGMAVVTQEDPS 450
           +DYAC+ SDCTAL YGSSCN L A GNASYAFNMY+Q+ +Q    C F G+A +T ++ S
Sbjct: 381 IDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLATITTKNIS 440

Query: 451 YGSCQFPVMIDYTFSSSSSSSSSSSFLDLLVAG 484
            G C FP+ I      +S++SS SS L LL+AG
Sbjct: 441 QGQCNFPIQI-----VASTASSFSSSLVLLIAG 466

BLAST of CmoCh05G013370 vs. TAIR10
Match: AT1G64760.1 (AT1G64760.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 535.8 bits (1379), Expect = 2.8e-152
Identity = 268/464 (57.76%), Postives = 335/464 (72.20%), Query Frame = 1

Query: 22  LTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVM 81
           L ++ NG  +GVNWGTMATH+LPP+ VV+ML++N   K+KLF+A++  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTL 141
           +AIPN  L+VM+    A   WV  NVT Y + GGV I +VAVGNEPFLKSYNG ++  T 
Sbjct: 78  VAIPNDQLKVMTSYDRAKD-WVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDLTIEILHFLS 201
           PAL NIQ ALN+AGLG+ VKAT+P NAD Y+SP SNPVPSAG FRPD+     +I+ FL 
Sbjct: 138 PALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLG 197

Query: 202 INNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNVFDANFDTLVSALTK 261
            NNAP T+NIYPFLSLYGND FP+++AFFDG  + I D  + YTNVFDANFDTLVS+L  
Sbjct: 198 KNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKA 257

Query: 262 AGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLI 321
            G+ DM IIVGEVGWPT+GDKHAN  +A RF  GLL    + +GTP R   I++YLF L+
Sbjct: 258 VGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLL 317

Query: 322 DENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVKRWCVLNPNV 381
           DE+AKSI PG FERHWGIFKFDG+PK+ +DLSG    K LI  + V Y+  +WC  NP  
Sbjct: 318 DEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEA 377

Query: 382 KDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNSQKSWNCDFSGM 441
           KD   LA ++DYAC+ SDCTAL YGSSCN L A GNASYAFNM++Q+ +Q    C F G+
Sbjct: 378 KDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGL 437

Query: 442 AVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLLVAGFM 486
           A +T ++ S G C FP+ I    SS+SS S SS  L +L+  F+
Sbjct: 438 ATITTQNISQGQCNFPIQI--VASSASSFSCSSYSLVVLIVWFL 475

BLAST of CmoCh05G013370 vs. TAIR10
Match: AT3G04010.1 (AT3G04010.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 492.3 bits (1266), Expect = 3.6e-139
Identity = 250/485 (51.55%), Postives = 335/485 (69.07%), Query Frame = 1

Query: 11  NWIFIALILLLLTVVPNGFS-VGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRI 70
           N   + +I+  +  +  G S +GVNWGTMA+HQLPP+ VV+ML++N  +K+KLF+A+   
Sbjct: 12  NSAVVFIIISAVCFLSGGVSGLGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNT 71

Query: 71  LEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFL 130
           + AL G+ +EVM+AIPN +L  M  + + A  WV  NV+ + ++ GVKIKYVAVGNEPFL
Sbjct: 72  MGALAGSGVEVMVAIPNDLLLAMG-NYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFL 131

Query: 131 KSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDV 190
            +YNG ++  T PAL NIQ ALN+AG+G   KAT+P NAD YNSP  N VPSAG FR D+
Sbjct: 132 TAYNGSFINLTYPALFNIQTALNEAGVGDFTKATVPLNADVYNSPPDNQVPSAGRFRSDI 191

Query: 191 RDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNVFD 250
                +I++FL+ N APFTVNIYPFLSLY +  FP ++AFFDG   ++ D  + YTNVFD
Sbjct: 192 IQEMTQIVNFLAQNKAPFTVNIYPFLSLYLSSDFPFEYAFFDGQ-NTVNDNGVIYTNVFD 251

Query: 251 ANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPAR 310
           A+FDTL+++L    + +M++IVGEVGWPTDGDK+AN+ NA+RF  GLL    +  GTP R
Sbjct: 252 ASFDTLLASLNALNHGNMEVIVGEVGWPTDGDKNANVPNAERFYSGLLPRLANNVGTPMR 311

Query: 311 VGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRY 370
            G I++YLF  IDE+AKS+ PG+FERHWGIFKFDG+PK+ +D  G   +K L   + V+Y
Sbjct: 312 KGYIEVYLFGFIDEDAKSVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQY 371

Query: 371 MVKRWCVLNPNVK-DWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQM 430
            + +WC+ NPN + +   L  +++YACS SDCTAL YGSSC  L A GNASYAFNMY+Q+
Sbjct: 372 FLNQWCMFNPNGRGNMSRLGDNINYACSHSDCTALGYGSSCGNLDANGNASYAFNMYFQV 431

Query: 431 NSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLLVAGFMGFL 490
            +Q++  CDF G+A +T ++ S G C FP+ I    S     S  S     LV   M  L
Sbjct: 432 QNQEAQACDFEGLATITTQNISQGQCNFPIQIGEPSSGHYDYSYGSMVRLCLV---MSGL 491

Query: 491 VFLFL 494
           VFL +
Sbjct: 492 VFLLI 491

BLAST of CmoCh05G013370 vs. TAIR10
Match: AT5G18220.1 (AT5G18220.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 490.3 bits (1261), Expect = 1.4e-138
Identity = 251/468 (53.63%), Postives = 326/468 (69.66%), Query Frame = 1

Query: 18  ILLLLTVVP----NGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEAL 77
           I L +T+V     +  ++GVNWGTM+THQLPP+ VV+ML++N  +K+KLF+A+   + AL
Sbjct: 13  IFLCITIVSLLSGDVSALGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADTNTMVAL 72

Query: 78  IGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYN 137
            G+ IEVM+AIPN  L+ M     A   WV  N+T +  +  VKIKYVAVGNEPFL +YN
Sbjct: 73  AGSGIEVMVAIPNDQLKAMGSYNRAKD-WVRRNITRF--NDDVKIKYVAVGNEPFLTAYN 132

Query: 138 GIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDLT 197
           G ++  T PAL NIQ+ALN+AG+G  +KAT+P NAD YNSP  NPVPSAG FR D+ +  
Sbjct: 133 GSFINLTYPALFNIQKALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIFEEM 192

Query: 198 IEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDGDLTYTNVFDANFD 257
             I++FL+ N APFTVNIYPFLSLY +  FP D+AFF+G   ++ D  + YTNVFDANFD
Sbjct: 193 KLIVNFLAHNKAPFTVNIYPFLSLYLSSDFPFDYAFFNGQ-NTVSDNGVIYTNVFDANFD 252

Query: 258 TLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGII 317
           TL+++L   G+ DM +IVGEVGWPTDGDK+ANI NA+RF  GLL    +  GTP R G I
Sbjct: 253 TLLASLKALGHGDMTVIVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMRPGYI 312

Query: 318 DIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVKR 377
           ++YLF  IDE+AKSI PG+FERHWGIFK+DG+PK+  DLSG   +K L   + V+Y+  +
Sbjct: 313 EVYLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLRNQ 372

Query: 378 WCVLNPNVKDWEG----LAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMN 437
           WC+ NPN   +      L  +V+YAC+ SDCTAL YGSSC  L   GNASYAFNMY+Q+ 
Sbjct: 373 WCMFNPNALTFSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNMYFQVQ 432

Query: 438 SQKSWNCDFSGMAVVTQEDPSYGSCQFPVMI-DYTFSSSSSSSSSSSF 477
           +QK+  CDF G+A++T  + S   C FP+ I D T   S  +    SF
Sbjct: 433 NQKAEACDFEGLAIITTRNISREQCNFPIQIGDPTSGHSDHNHRFISF 476

BLAST of CmoCh05G013370 vs. NCBI nr
Match: gi|659071084|ref|XP_008458070.1| (PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo])

HSP 1 Score: 864.8 bits (2233), Expect = 7.5e-248
Identity = 431/491 (87.78%), Postives = 454/491 (92.46%), Query Frame = 1

Query: 10  RNWIF---IALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAE 69
           RN IF   + L+LLLL +VPNG SVGVNWGTMATHQLPPEKVVKMLQENGF KLKLFEA+
Sbjct: 3   RNCIFRILLVLLLLLLMIVPNGSSVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAD 62

Query: 70  DRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNE 129
           DRILEALIGTDIEVMLAIPNSML +MS+DP+AAA WVDSNVTAYTYHGGVKIKYVAVGNE
Sbjct: 63  DRILEALIGTDIEVMLAIPNSMLFLMSQDPQAAASWVDSNVTAYTYHGGVKIKYVAVGNE 122

Query: 130 PFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFR 189
           PFLKSYNG YLQ TLPALRNIQQALNDAGLGSKVKATIPFNAD YNSPDSNPVPSAG+FR
Sbjct: 123 PFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGEFR 182

Query: 190 PDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGT-YKSIKDGDLTYT 249
           PDVRDL IEILH+LSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGT YK IKD DLTYT
Sbjct: 183 PDVRDLAIEILHYLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTAYKPIKDRDLTYT 242

Query: 250 NVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEG 309
           NVFDANFDTLVSAL KAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEG
Sbjct: 243 NVFDANFDTLVSALAKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEG 302

Query: 310 TPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVE 369
           TPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIF+FDGKPKYELDL+G E +KGLI VE
Sbjct: 303 TPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLAGTEEEKGLIPVE 362

Query: 370 GVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMY 429
           GVRYM KRWCVLNPNV DWEGLA SVDYACSLSDCTALEYGSSCNQL+A+GNASYAFNMY
Sbjct: 363 GVRYMGKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMY 422

Query: 430 YQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSS--SSSSFLDLLVAG 489
           YQ+N+QKSWNCDF G+AVVTQ+DPSYG+C FP+MIDYT SSSSSSS      F DLLVA 
Sbjct: 423 YQVNNQKSWNCDFDGLAVVTQQDPSYGNCLFPIMIDYTSSSSSSSSFLVDERFFDLLVAI 482

Query: 490 FMGFLVFLFLE 495
           FMG +VFL ++
Sbjct: 483 FMGIVVFLMVQ 493

BLAST of CmoCh05G013370 vs. NCBI nr
Match: gi|778665975|ref|XP_004139424.2| (PREDICTED: glucan endo-1,3-beta-glucosidase 8 isoform X1 [Cucumis sativus])

HSP 1 Score: 857.8 bits (2215), Expect = 9.1e-246
Identity = 425/491 (86.56%), Postives = 452/491 (92.06%), Query Frame = 1

Query: 10  RNWIF---IALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAE 69
           RN IF   + L+ LL+ +VPNG SVGVNWGTMATHQLPPEKVVKMLQENGF KLKLFEA+
Sbjct: 3   RNCIFRILLVLLWLLMMIVPNGLSVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAD 62

Query: 70  DRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNE 129
           DRILEALIG+DIEVMLAIPNSML +MS+DP AAA WVDSNVTAYTYHGGVKIKYVAVGNE
Sbjct: 63  DRILEALIGSDIEVMLAIPNSMLFLMSQDPHAAASWVDSNVTAYTYHGGVKIKYVAVGNE 122

Query: 130 PFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFR 189
           PFLKSYNG YL  TLPALRNIQQ+LNDAGLGSKVKATIPFNAD YNSPDSNPVPSAG+FR
Sbjct: 123 PFLKSYNGTYLPLTLPALRNIQQSLNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGEFR 182

Query: 190 PDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGT-YKSIKDGDLTYT 249
           PDVRDLTIEILH+LSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGT Y+ IKD DLTYT
Sbjct: 183 PDVRDLTIEILHYLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTAYRPIKDRDLTYT 242

Query: 250 NVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEG 309
           NVFDANFDTLVSAL KAGYPDMKIIVGEVGWPTDGDKHAN+QNAKRFNQGLLRHALSG+G
Sbjct: 243 NVFDANFDTLVSALAKAGYPDMKIIVGEVGWPTDGDKHANVQNAKRFNQGLLRHALSGQG 302

Query: 310 TPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVE 369
           TPARVGIID+YLFSLIDENAKSIEPGSFERHWGIF+FDGKPKYELDL+G E +KGLI VE
Sbjct: 303 TPARVGIIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLAGTEEEKGLIPVE 362

Query: 370 GVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMY 429
           GVRYM KRWCVLNPNV DWEGLA SVDYACSLSDCTALEYGSSCNQLSA+GNASYAFNMY
Sbjct: 363 GVRYMGKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLSAQGNASYAFNMY 422

Query: 430 YQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSS--SSSSFLDLLVAG 489
           YQ+NSQKSWNCDF G+AVVTQ+DPSYG+CQFP+MIDYT SSSSSSS      F DLLVA 
Sbjct: 423 YQVNSQKSWNCDFDGLAVVTQQDPSYGNCQFPIMIDYTSSSSSSSSFLVYERFSDLLVAV 482

Query: 490 FMGFLVFLFLE 495
           FMG +VF  ++
Sbjct: 483 FMGIVVFFIVQ 493

BLAST of CmoCh05G013370 vs. NCBI nr
Match: gi|700205520|gb|KGN60639.1| (hypothetical protein Csa_2G005360 [Cucumis sativus])

HSP 1 Score: 852.0 bits (2200), Expect = 5.0e-244
Identity = 418/474 (88.19%), Postives = 442/474 (93.25%), Query Frame = 1

Query: 24  VVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKLKLFEAEDRILEALIGTDIEVMLA 83
           +VPNG SVGVNWGTMATHQLPPEKVVKMLQENGF KLKLFEA+DRILEALIG+DIEVMLA
Sbjct: 2   IVPNGLSVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEADDRILEALIGSDIEVMLA 61

Query: 84  IPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGIYLQSTLPA 143
           IPNSML +MS+DP AAA WVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNG YL  TLPA
Sbjct: 62  IPNSMLFLMSQDPHAAASWVDSNVTAYTYHGGVKIKYVAVGNEPFLKSYNGTYLPLTLPA 121

Query: 144 LRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVPSAGDFRPDVRDLTIEILHFLSIN 203
           LRNIQQ+LNDAGLGSKVKATIPFNAD YNSPDSNPVPSAG+FRPDVRDLTIEILH+LSIN
Sbjct: 122 LRNIQQSLNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGEFRPDVRDLTIEILHYLSIN 181

Query: 204 NAPFTVNIYPFLSLYGNDYFPIDFAFFDGT-YKSIKDGDLTYTNVFDANFDTLVSALTKA 263
           NAPFTVNIYPFLSLYGNDYFPIDFAFFDGT Y+ IKD DLTYTNVFDANFDTLVSAL KA
Sbjct: 182 NAPFTVNIYPFLSLYGNDYFPIDFAFFDGTAYRPIKDRDLTYTNVFDANFDTLVSALAKA 241

Query: 264 GYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLID 323
           GYPDMKIIVGEVGWPTDGDKHAN+QNAKRFNQGLLRHALSG+GTPARVGIID+YLFSLID
Sbjct: 242 GYPDMKIIVGEVGWPTDGDKHANVQNAKRFNQGLLRHALSGQGTPARVGIIDVYLFSLID 301

Query: 324 ENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKGLIAVEGVRYMVKRWCVLNPNVK 383
           ENAKSIEPGSFERHWGIF+FDGKPKYELDL+G E +KGLI VEGVRYM KRWCVLNPNV 
Sbjct: 302 ENAKSIEPGSFERHWGIFEFDGKPKYELDLAGTEEEKGLIPVEGVRYMGKRWCVLNPNVN 361

Query: 384 DWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASYAFNMYYQMNSQKSWNCDFSGMA 443
           DWEGLA SVDYACSLSDCTALEYGSSCNQLSA+GNASYAFNMYYQ+NSQKSWNCDF G+A
Sbjct: 362 DWEGLADSVDYACSLSDCTALEYGSSCNQLSAQGNASYAFNMYYQVNSQKSWNCDFDGLA 421

Query: 444 VVTQEDPSYGSCQFPVMIDYTFSSSSSSS--SSSSFLDLLVAGFMGFLVFLFLE 495
           VVTQ+DPSYG+CQFP+MIDYT SSSSSSS      F DLLVA FMG +VF  ++
Sbjct: 422 VVTQQDPSYGNCQFPIMIDYTSSSSSSSSFLVYERFSDLLVAVFMGIVVFFIVQ 475

BLAST of CmoCh05G013370 vs. NCBI nr
Match: gi|1009160426|ref|XP_015898343.1| (PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Ziziphus jujuba])

HSP 1 Score: 712.6 bits (1838), Expect = 4.7e-202
Identity = 344/496 (69.35%), Postives = 413/496 (83.27%), Query Frame = 1

Query: 1   MSESVLRFHRNWIFIALILLLLTVVPNGFS---VGVNWGTMATHQLPPEKVVKMLQENGF 60
           M++  +  H   + + +IL+ +T +  G S   VGVNWGTMATHQLPPEKVV+ML +NGF
Sbjct: 92  MAQERMVDHNYVMAVVVILVFMTKMSGGGSSGTVGVNWGTMATHQLPPEKVVQMLMDNGF 151

Query: 61  RKLKLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVK 120
           RKLKLFEA++RIL ALIGT IEVMLA+PNSMLQ MSEDP AAA WVD+NVT+Y+Y GGV 
Sbjct: 152 RKLKLFEADERILGALIGTPIEVMLAVPNSMLQEMSEDPVAAASWVDANVTSYSYTGGVN 211

Query: 121 IKYVAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSN 180
           +KYVAVGNEPFL+SYNG YLQSTLPAL+NIQQALNDA LGS+VKAT+PFNAD YNSPD N
Sbjct: 212 VKYVAVGNEPFLESYNGSYLQSTLPALKNIQQALNDAHLGSRVKATVPFNADIYNSPDWN 271

Query: 181 PVPSAGDFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSI 240
            VPSAGDFRP+VRD+TIEI+ +L  N APFTVNIYPFLSLYGNDYFP+++AFFDG  K +
Sbjct: 272 LVPSAGDFRPEVRDITIEIIQYLHTNEAPFTVNIYPFLSLYGNDYFPVEYAFFDGKSKPL 331

Query: 241 KDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLL 300
           KDGD  YTN FDAN+DTLV+AL +AG+PD++IIVGEVGWPTDGDKHAN+Q AKRFNQG++
Sbjct: 332 KDGDRVYTNAFDANYDTLVAALNRAGFPDIRIIVGEVGWPTDGDKHANVQYAKRFNQGMV 391

Query: 301 RHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEA 360
           R ALSG GTPAR G I++YLFSLIDENAK I PG+FERHWGIF++DGKPKYELDLSG   
Sbjct: 392 RRALSGNGTPARKGQIEVYLFSLIDENAKLISPGNFERHWGIFEYDGKPKYELDLSGSRE 451

Query: 361 QKGLIAVEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGN 420
            KGL+  + V+Y+++RWCVLNP+VKD + L  S+DYACS SDCTAL +GSSCN LS EGN
Sbjct: 452 DKGLVPADDVKYLLRRWCVLNPDVKDLDDLPDSIDYACSQSDCTALGFGSSCNNLSVEGN 511

Query: 421 ASYAFNMYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFL 480
           ASYAFNMYYQ+N QKSWNCDFSG+A+VT +DPS G CQFP+MI + F       +++  L
Sbjct: 512 ASYAFNMYYQLNDQKSWNCDFSGLAMVTDDDPSVGDCQFPIMISHGF---PILLANNGVL 571

Query: 481 DLLVAGFMGFLVFLFL 494
           DL++   +G ++F+ L
Sbjct: 572 DLVMNIVLGCILFMIL 584

BLAST of CmoCh05G013370 vs. NCBI nr
Match: gi|1009160094|ref|XP_015898166.1| (PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Ziziphus jujuba])

HSP 1 Score: 712.6 bits (1838), Expect = 4.7e-202
Identity = 344/496 (69.35%), Postives = 413/496 (83.27%), Query Frame = 1

Query: 1   MSESVLRFHRNWIFIALILLLLTVVPNGFS---VGVNWGTMATHQLPPEKVVKMLQENGF 60
           M++  +  H   + + +IL+ +T +  G S   VGVNWGTMATHQLPPEKVV+ML +NGF
Sbjct: 1   MAQERMVDHNYVMAVVVILVFMTKMSGGGSSGTVGVNWGTMATHQLPPEKVVQMLMDNGF 60

Query: 61  RKLKLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVK 120
           RKLKLFEA++RIL ALIGT IEVMLA+PNSMLQ MSEDP AAA WVD+NVT+Y+Y GGV 
Sbjct: 61  RKLKLFEADERILGALIGTPIEVMLAVPNSMLQEMSEDPVAAASWVDANVTSYSYTGGVN 120

Query: 121 IKYVAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSN 180
           +KYVAVGNEPFL+SYNG YLQSTLPAL+NIQQALNDA LGS+VKAT+PFNAD YNSPD N
Sbjct: 121 VKYVAVGNEPFLESYNGSYLQSTLPALKNIQQALNDAHLGSRVKATVPFNADIYNSPDWN 180

Query: 181 PVPSAGDFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSI 240
            VPSAGDFRP+VRD+TIEI+ +L  N APFTVNIYPFLSLYGNDYFP+++AFFDG  K +
Sbjct: 181 LVPSAGDFRPEVRDITIEIIQYLHTNEAPFTVNIYPFLSLYGNDYFPVEYAFFDGKSKPL 240

Query: 241 KDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLL 300
           KDGD  YTN FDAN+DTLV+AL +AG+PD++IIVGEVGWPTDGDKHAN+Q AKRFNQG++
Sbjct: 241 KDGDRVYTNAFDANYDTLVAALNRAGFPDIRIIVGEVGWPTDGDKHANVQYAKRFNQGMV 300

Query: 301 RHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEA 360
           R ALSG GTPAR G I++YLFSLIDENAK I PG+FERHWGIF++DGKPKYELDLSG   
Sbjct: 301 RRALSGNGTPARKGQIEVYLFSLIDENAKLISPGNFERHWGIFEYDGKPKYELDLSGSRE 360

Query: 361 QKGLIAVEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGN 420
            KGL+  + V+Y+++RWCVLNP+VKD + L  S+DYACS SDCTAL +GSSCN LS EGN
Sbjct: 361 DKGLVPADDVKYLLRRWCVLNPDVKDLDDLPDSIDYACSQSDCTALGFGSSCNNLSVEGN 420

Query: 421 ASYAFNMYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFL 480
           ASYAFNMYYQ+N QKSWNCDFSG+A+VT +DPS G CQFP+MI + F       +++  L
Sbjct: 421 ASYAFNMYYQLNDQKSWNCDFSGLAMVTDDDPSVGDCQFPIMISHGF---PILLANNGVL 480

Query: 481 DLLVAGFMGFLVFLFL 494
           DL++   +G ++F+ L
Sbjct: 481 DLVMNIVLGCILFMIL 493

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E138_ARATH5.0e-15157.76Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana GN=At1g64760 PE=1 SV=... [more]
E136_ARATH5.3e-13751.88Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana GN=At5g58090 PE=1 SV=... [more]
E135_ARATH1.7e-13552.07Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana GN=At4g31140 PE=1 SV=... [more]
E139_ARATH1.2e-9640.72Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana GN=At5g58480 PE=1 SV=... [more]
E137_ARATH4.3e-6234.99Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana GN=At4g34480 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LHZ1_CUCSA3.5e-24488.19Uncharacterized protein OS=Cucumis sativus GN=Csa_2G005360 PE=3 SV=1[more]
M5W8Z4_PRUPE1.7e-19871.07Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017632mg PE=3 SV=1[more]
A0A068U4D9_COFCA2.3e-19571.12Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00041929001 PE=3 SV=1[more]
A0A072USM3_MEDTR1.5e-19467.89Glucan endo-1,3-beta-glucosidase-like protein OS=Medicago truncatula GN=MTR_4g11... [more]
W9QRM7_9ROSA1.9e-19470.46Glucan endo-1,3-beta-glucosidase 8 OS=Morus notabilis GN=L484_020860 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G24330.19.3e-18062.65 O-Glycosyl hydrolases family 17 protein[more]
AT2G19440.13.0e-15459.38 O-Glycosyl hydrolases family 17 protein[more]
AT1G64760.12.8e-15257.76 O-Glycosyl hydrolases family 17 protein[more]
AT3G04010.13.6e-13951.55 O-Glycosyl hydrolases family 17 protein[more]
AT5G18220.11.4e-13853.63 O-Glycosyl hydrolases family 17 protein[more]
Match NameE-valueIdentityDescription
gi|659071084|ref|XP_008458070.1|7.5e-24887.78PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo][more]
gi|778665975|ref|XP_004139424.2|9.1e-24686.56PREDICTED: glucan endo-1,3-beta-glucosidase 8 isoform X1 [Cucumis sativus][more]
gi|700205520|gb|KGN60639.1|5.0e-24488.19hypothetical protein Csa_2G005360 [Cucumis sativus][more]
gi|1009160426|ref|XP_015898343.1|4.7e-20269.35PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Ziziphus jujuba][more]
gi|1009160094|ref|XP_015898166.1|4.7e-20269.35PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000490Glyco_hydro_17
IPR012946X8
IPR013781Glycoside hydrolase, catalytic domain
IPR017853Glycoside_hydrolase_SF
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0030488 tRNA methylation
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005634 nucleus
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0052906 tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G013370.1CmoCh05G013370.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 31..351
score: 4.7
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 267..280
scor
IPR012946X8 domainPFAMPF07983X8coord: 374..442
score: 4.2
IPR012946X8 domainSMARTSM00768X8_clscoord: 373..456
score: 1.4
IPR013781Glycoside hydrolase, catalytic domainGENE3DG3DSA:3.20.20.80coord: 31..352
score: 5.0E
IPR017853Glycoside hydrolase superfamilyunknownSSF51445(Trans)glycosidasescoord: 31..351
score: 1.72
NoneNo IPR availablePANTHERPTHR32227FAMILY NOT NAMEDcoord: 25..444
score: 4.0E
NoneNo IPR availablePANTHERPTHR32227:SF82SUBFAMILY NOT NAMEDcoord: 25..444
score: 4.0E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh05G013370CmoCh12G012630Cucurbita moschata (Rifu)cmocmoB148