CmoCh05G006840 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh05G006840
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Gibberellin 3 beta-hydroxylase family protein
Location	Cmo_Chr05 : 3486188 .. 3487341 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGACCAAAAGCTTCCTCCCGCAAGCCTTGTGGTTCCTCTAGACTTCGACTCGGTTCAGACTATTCCTGAATCTCACTTATGGCCTCACCCCGATGAATCATCGCAGAAGATCGAATCAGACAAAATAGTCTCGGTCCCGGTTGTCGATCTTAACGACGATAATGTGTTGGAGCTCATAGGAAAAGCCTGCAAGGAGTGGGGAATGTTTCAGTTGATTAATCATGGTATCCCGAAAACCCTAATAGCCGAAACCGAGGAGGTGGCTCAATGTCTTTTCGCTCTTCCAAAAAGCCAGAAGATGAAGGCATTACAGGTTCCCGGAACCTCCACTGGCTACTGCATAGCCAGATTTTCGAAGTATTATGAGAAACTTATGTGGCATGAAGGATTCTCCATCATAGGTTCTCCAGTTGATGATTTTAAGAAACTCTGGCCATCGGACTACCAACGATTCTGGTAAATCCAGGTTTATGTTTAACTGTTTATTACTCTAAATCCCAACCATTTTTATATGCCCAAATTGGATTCTTACTTTCTTTTTCTTTTTCTTTTTTTTAATCAAGTGATAAAATGGAAGAATACCAACTGAAAATGAAGGCTTTAGGTGATAAACTAATAAGGTTAATCTTCAAGTTTCTTGGCATCTTCGATGAAGAGATGGTGAAATTGTTGTCTTACATCGACCCGACCACCGAAAAACCCCACATGCTTTTCCGCTTGAACTCGTATCCGCCATGCCCTGATCCAAGCAAAGTCATTGGCCTCGCGACCCACACTGACACCTCCCTCCTCACAATGCTTCACCAGGCACGCAAATATGGGCTACAGATCTTCAACGAGAAAGAAGGCTGGATTCCGCTGGCTCGGAGGAGCGACACTCTCATTATTAACATCGGCGATTTACTCCAAATTATATCGAACGGGCGGTTTCATAGCATTCCTCACCGGGTGATGATACAAGAGACTGAGGAGACTACGATGTCGATGGCATTTTTCTATTATCCACCAAGTTATTTAAACGTATCACCTTATTGTAAGCCATTGAGTGAAACCCCACATACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCGCCATCAAGGCCAACAACTTGGGGGAGGGAATTGCTGCCCTAACAATATGA

mRNA sequence

ATGGCAGACCAAAAGCTTCCTCCCGCAAGCCTTGTGGTTCCTCTAGACTTCGACTCGGTTCAGACTATTCCTGAATCTCACTTATGGCCTCACCCCGATGAATCATCGCAGAAGATCGAATCAGACAAAATAGTCTCGGTCCCGGTTGTCGATCTTAACGACGATAATGTGTTGGAGCTCATAGGAAAAGCCTGCAAGGAGTGGGGAATGTTTCAGTTGATTAATCATGGTATCCCGAAAACCCTAATAGCCGAAACCGAGGAGGTGGCTCAATGTCTTTTCGCTCTTCCAAAAAGCCAGAAGATGAAGGCATTACAGGTTCCCGGAACCTCCACTGGCTACTGCATAGCCAGATTTTCGAAGTATTATGAGAAACTTATGTGGCATGAAGGATTCTCCATCATAGGTTCTCCAGTTGATGATTTTAAGAAACTCTGGCCATCGGACTACCAACGATTCTGGTTAATCTTCAAGTTTCTTGGCATCTTCGATGAAGAGATGGTGAAATTGTTGTCTTACATCGACCCGACCACCGAAAAACCCCACATGCTTTTCCGCTTGAACTCGTATCCGCCATGCCCTGATCCAAGCAAAGTCATTGGCCTCGCGACCCACACTGACACCTCCCTCCTCACAATGCTTCACCAGGCACGCAAATATGGGCTACAGATCTTCAACGAGAAAGAAGGCTGGATTCCGCTGGCTCGGAGGAGCGACACTCTCATTATTAACATCGGCGATTTACTCCAAATTATATCGAACGGGCGGTTTCATAGCATTCCTCACCGGGTGATGATACAAGAGACTGAGGAGACTACGATGTCGATGGCATTTTTCTATTATCCACCAAGTTATTTAAACGTATCACCTTATTGTAAGCCATTGAGTGAAACCCCACATACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCGCCATCAAGGCCAACAACTTGGGGGAGGGAATTGCTGCCCTAACAATATGA

Coding sequence (CDS)

ATGGCAGACCAAAAGCTTCCTCCCGCAAGCCTTGTGGTTCCTCTAGACTTCGACTCGGTTCAGACTATTCCTGAATCTCACTTATGGCCTCACCCCGATGAATCATCGCAGAAGATCGAATCAGACAAAATAGTCTCGGTCCCGGTTGTCGATCTTAACGACGATAATGTGTTGGAGCTCATAGGAAAAGCCTGCAAGGAGTGGGGAATGTTTCAGTTGATTAATCATGGTATCCCGAAAACCCTAATAGCCGAAACCGAGGAGGTGGCTCAATGTCTTTTCGCTCTTCCAAAAAGCCAGAAGATGAAGGCATTACAGGTTCCCGGAACCTCCACTGGCTACTGCATAGCCAGATTTTCGAAGTATTATGAGAAACTTATGTGGCATGAAGGATTCTCCATCATAGGTTCTCCAGTTGATGATTTTAAGAAACTCTGGCCATCGGACTACCAACGATTCTGGTTAATCTTCAAGTTTCTTGGCATCTTCGATGAAGAGATGGTGAAATTGTTGTCTTACATCGACCCGACCACCGAAAAACCCCACATGCTTTTCCGCTTGAACTCGTATCCGCCATGCCCTGATCCAAGCAAAGTCATTGGCCTCGCGACCCACACTGACACCTCCCTCCTCACAATGCTTCACCAGGCACGCAAATATGGGCTACAGATCTTCAACGAGAAAGAAGGCTGGATTCCGCTGGCTCGGAGGAGCGACACTCTCATTATTAACATCGGCGATTTACTCCAAATTATATCGAACGGGCGGTTTCATAGCATTCCTCACCGGGTGATGATACAAGAGACTGAGGAGACTACGATGTCGATGGCATTTTTCTATTATCCACCAAGTTATTTAAACGTATCACCTTATTGTAAGCCATTGAGTGAAACCCCACATACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCGCCATCAAGGCCAACAACTTGGGGGAGGGAATTGCTGCCCTAACAATATGA

Homology

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BLAST of CmoCh05G006840 vs. Swiss-Prot
Match: G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 8.4e-70
Identity = 135/338 (39.94%), Postives = 198/338 (58.58%), Query Frame = 1


Query: 16  DFDSVQTIPESHLWPHPDE----SSQKIESDKIVSVPVVDLNDDNVLELIGKACKEWGMF 75
           DF+S+Q +PES+ W H D+     S  I  +   +VPV+DLND N  +LIG ACK WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 76  QLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWHEG 135
           Q++NHGIP +L+ + + + Q LF+LP  QK KA + P   +GY IAR S ++ KLMW+EG
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVSGYGIARISSFFPKLMWYEG 140

Query: 136 FSIIGSPVDDFKKLWPSDYQRFWLIFKFLGIFDEEMVKLLSYI----------------- 195
           F+I+GSP+D F++LWP DY RF  I   +  +DE M KL   +                 
Sbjct: 141 FTIVGSPLDHFRELWPQDYTRFCDI---VVQYDETMKKLAGTLMCLMLDSLGITKEDIKW 200

Query: 196 ---DPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIFNEKEGW 255
                  EK     +LNSYP CPDP   +GLA HTD++ LT+L Q    GLQ+  E  GW
Sbjct: 201 AGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGW 260

Query: 256 IPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSYLNVSPY 315
           I +      L++N+GDL  I+SNG + S+ HRV++  T +   S+A+ Y PPS + + P+
Sbjct: 261 ITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQ-RFSVAYLYGPPSNVEICPH 320

Query: 316 CKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
            K +  T   P+Y+ V   EY   KA +  + ++++ +
Sbjct: 321 AKLIGPT-KPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

[Close]

BLAST of CmoCh05G006840 vs. Swiss-Prot
Match: G3OX2_ARATH (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 224.9 bits (572), Expect = 1.3e-57
Identity = 117/335 (34.93%), Postives = 191/335 (57.01%), Query Frame = 1


Query: 14  PLDFDSVQTIPESHLW-PHPDESSQKIESDKIVSVPVVDLNDDNVLELIGKACKEWGMFQ 73
           P DF S   +P+S+ W P  D       SD+  ++P++DL+D +V  L+G AC  WG FQ
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWHEGF 133
           + NHG+P  L+ + E +   LF LP  +K+KA +     +GY +AR + ++ K MW EGF
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKMWSEGF 140

Query: 134 SIIGSPVDDFKKLWPSDYQRFWLIFKFLGIFDEEMVKL------------------LSYI 193
           ++IGSP+ DF+KLWPS + ++  I +    ++E M KL                  + + 
Sbjct: 141 TVIGSPLHDFRKLWPSHHLKYCEIIE---EYEEHMQKLAAKLMWFALGSLGVEEKDIQWA 200

Query: 194 DPTT--EKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIFNEKEGWI 253
            P +  +    + +LN YP CP+P + +GLA HTD++L+T+L+Q    GLQ+F +  GW+
Sbjct: 201 GPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWV 260

Query: 254 PLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSYLNVSPYC 313
                  +L++N+GDLL I++NG F S+ HR  +     +  SMA+ + PPS + +SP  
Sbjct: 261 TAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV-RSRFSMAYLWGPPSDIMISPLP 320

Query: 314 KPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAAL 328
           K L +   +P+Y  +  K+Y A KA +  + ++ +
Sbjct: 321 K-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSII 345

[Close]

BLAST of CmoCh05G006840 vs. Swiss-Prot
Match: G3OX1_ARATH (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 223.0 bits (567), Expect = 4.8e-57
Identity = 111/334 (33.23%), Postives = 185/334 (55.39%), Query Frame = 1


Query: 16  DFDSVQTIPESHLWPHPDE---SSQKIESDKIVSVPVVDLNDDNVLELIGKACKEWGMFQ 75
           DF S++ +P+S+ W   D+   S+         ++P++DL+  +    IG AC+ WG FQ
Sbjct: 23  DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHACRTWGAFQ 82

Query: 76  LINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWHEGF 135
           + NHG+P  L+ + E +   LF LP  +K+K+ +     +GY +AR + ++ K MW EGF
Sbjct: 83  ISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFNKQMWSEGF 142

Query: 136 SIIGSPVDDFKKLWPSDYQRF-------------------WLIFKFLGIFDEEMVKLLSY 195
           +I GSP++DF+KLWP  +  +                   WL    LG+ +E++    + 
Sbjct: 143 TITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEEDIE--WAS 202

Query: 196 IDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIFNEKEGWIP 255
           +           +LN YP CP+P + +GLA HTD++LLT+L+Q    GLQ+F +  GW+ 
Sbjct: 203 LSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVT 262

Query: 256 LARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSYLNVSPYCK 315
           +     +L++N+GDL  I+SNG F S+ HR  + +T    +S+AF + P S + +SP  K
Sbjct: 263 VPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK 322

Query: 316 PLSETPHTPIYKGVNVKEYFAIKANNLGEGIAAL 328
            +S    +P+Y+ V  KEY   KA +  + ++ +
Sbjct: 323 LVSPV-ESPLYQSVTWKEYLRTKATHFNKALSMI 352

[Close]

BLAST of CmoCh05G006840 vs. Swiss-Prot
Match: G3OX3_ARATH (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.4e-53
Identity = 119/339 (35.10%), Postives = 189/339 (55.75%), Query Frame = 1


Query: 12  VVPLDFDSVQTIPESHLW--PHPDESSQKIESDKIVSVPVVDLNDDNVLELIGKACKEWG 71
           ++PLDF + +T+P+SH+W  P P+ +S  I        PV+ L++     L+ +AC+EWG
Sbjct: 18  IIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEWG 77

Query: 72  MFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWH 131
           +F + +HG+  +L+   +   + LF+LP  +K+ A++ P  STGY + R S +Y+KLMW 
Sbjct: 78  VFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWS 137

Query: 132 EGFSIIGSPVDDFKK-LWPSDYQRFWLIF-KFLGIFDEEMVKLLSYI------------- 191
           EGFS++GS +      LWP D+  F  +  ++    D+   +L+S +             
Sbjct: 138 EGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLGW 197

Query: 192 ---DPT---TEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIFNEK 251
              D T   T+      +LNSYP CPDP   +GLA HTD+SLLT+L+Q    GL+I + +
Sbjct: 198 LVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQ 257

Query: 252 E---GWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSY 311
           E    WI +     +L++ +GDL  IISNG+F S  HR ++ +T    +S A+F  PP  
Sbjct: 258 EEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH-RVSAAYFAGPPKN 317

Query: 312 LNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGI 325
           L + P     S+  H PIY+ +  +EY A KA +  + +
Sbjct: 318 LQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKAL 344

[Close]

BLAST of CmoCh05G006840 vs. Swiss-Prot
Match: G3O23_WHEAT (Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum GN=GA3ox2-3 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.0e-51
Identity = 117/343 (34.11%), Postives = 175/343 (51.02%), Query Frame = 1


Query: 16  DFDSVQTIPESHLWP----HP--DESSQKIESDKIVSVPVVDLNDDNVLELIGKACKEWG 75
           DF + + +PE+H WP    HP  D S    E D   +VPVVD+ D    E +G A ++WG
Sbjct: 15  DFRAARRVPETHAWPGLHDHPVVDGSGAGGEPD---AVPVVDMRDPFAAEAVGLAAQDWG 74

Query: 76  MFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWH 135
            F L+ HG+P  L+   E     +FALP S+KM+A++ PG S GY     S ++ K MW 
Sbjct: 75  AFLLVGHGVPLDLLVRVEAAIAGMFALPASEKMRAVRRPGDSCGYGSPPISSFFSKCMWS 134

Query: 136 EGFSIIGSPVD---DFKKLWPS---DYQRFWLIF------------KFLGIF------DE 195
           EG++   SP +   D +KLWP    DY+ F  +             K L +F        
Sbjct: 135 EGYTF--SPANLRSDLRKLWPKAGHDYRHFCAVMEEFHREMRALADKLLELFLVALGLTG 194

Query: 196 EMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIF 255
           E V  +       E       LN YP CPDP + +GL  HTD+   T + Q+   GLQ+F
Sbjct: 195 EQVAAVESEQKIAETMTATMHLNWYPKCPDPKRALGLIAHTDSGFFTFVLQSLVPGLQLF 254

Query: 256 NE-KEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPS 315
               + W+ +      +++N+GDL QI++NGRFHS+ HR ++   E   +S+ +F  PP+
Sbjct: 255 RHGPDRWVTVPAVPGAMVVNVGDLFQILTNGRFHSVYHRAVVNR-ESDRISLGYFLGPPA 314

Query: 316 YLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAAL 328
           ++ V+P  + L+ TP    Y+ V   EY  ++      G +AL
Sbjct: 315 HVKVAPLREALAGTP--AAYRAVTWPEYMGVRKKAFTTGASAL 349

[Close]

BLAST of CmoCh05G006840 vs. TrEMBL
Match: O24417_CUCMA (Gibberellin 2beta,3beta-hydroxylase OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 7.0e-148
Identity = 251/344 (72.97%), Postives = 286/344 (83.14%), Query Frame = 1


Query: 5   KLPPASLVVPLDFDSVQTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDNVLELIGKA 64
           KLP   L+ PLD ++V+ +PESH+WPH DESSQK +SDK VS+PVVD NDDNVLELIGKA
Sbjct: 3   KLPAVGLLTPLDLNTVEAVPESHVWPHLDESSQKFKSDKTVSIPVVDFNDDNVLELIGKA 62

Query: 65  CKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYE 124
           C+EWGMFQLINHGIPKTL AETEEVA+CLFALPKSQKMK L VPGT+ GYC+AR +K+++
Sbjct: 63  CEEWGMFQLINHGIPKTLTAETEEVARCLFALPKSQKMKTLNVPGTANGYCMARLTKHHD 122

Query: 125 KLMWHEGFSIIGSPVDDFKKLWPSDYQRFW-------------------LIFKFLGIFDE 184
           K+MWHEGF++IGSPVDDFKKLWPSDYQ F                    LIFK+LGI DE
Sbjct: 123 KMMWHEGFTVIGSPVDDFKKLWPSDYQPFCDKVEEYQLKMKALADKLISLIFKYLGISDE 182

Query: 185 EMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIF 244
           EMVK LSYIDP T KPH+  RLNS+PPCP+PSKV+GLA HTDTSL TMLHQAR+ GLQI 
Sbjct: 183 EMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVMGLAAHTDTSLFTMLHQARREGLQIL 242

Query: 245 NEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSY 304
           NEKEGW+PLA R D LIINIGD LQIISNGRFHS+PHRVMI+ETE+TTMSMA+F++PP +
Sbjct: 243 NEKEGWLPLAPRRDALIINIGDFLQIISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPGH 302

Query: 305 LNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
           L V+PYCKPLSET  TPIYKGVNVKEYF IKA   G+GIAALTI
Sbjct: 303 LYVAPYCKPLSETLQTPIYKGVNVKEYFIIKAKASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006840 vs. TrEMBL
Match: S0BE47_CUCSA (GA 3-oxidase OS=Cucumis sativus GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.3e-117
Identity = 209/350 (59.71%), Postives = 257/350 (73.43%), Query Frame = 1


Query: 1   MADQKLPPASLVVPLDFDSVQTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDNVLEL 60
           MADQ++PP S +VPLDF SVQT+PESHLWP+  E S  I+ DK VS+P++DL  D   EL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  IGKACKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPG--TSTGYCIAR 120
           +G+AC+EWGMFQLINHGI KTLI E EE  + LF LP +QKMK L+ PG  T+TGY +A 
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 FSKYYEKLMWHEGFSIIGSPVDDFKKLWPSDYQRFW-------------------LIFKF 180
            SKY+ KLMWHEGF+I+GSP+++ KKLWP+DY+RF                    LI KF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LGIFDEEMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARK 240
           LGI +EEM+KLL+  D +  KPHM  RLNSYPPCPDP +V+GLA HTDTSL T+LHQ   
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 YGLQIFNEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFF 300
            GLQIF +  GW+PL+  S TLI+NIGDLL I+SNGRF SI HRVMIQE +E   S+A+F
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YYPPSYLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
           Y PP  + +SPYCKPLS+TP  P+Y+ VNVKEYFAIKA   G+G+ A+ I
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of CmoCh05G006840 vs. TrEMBL
Match: Q9LM06_CITLA (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus GN=WGA3H PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 3.2e-116
Identity = 203/349 (58.17%), Postives = 259/349 (74.21%), Query Frame = 1


Query: 1   MADQKLPPASLVVPLDFDSVQTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDNVLEL 60
           MADQ+LPP S +VPLDF+S+QT+PESHLWP+  E S  I+ D+ +S+P++D  DD   EL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  IGKACKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPG-TSTGYCIARF 120
           +G+AC++WGMFQLINHG+PKTLI E EE  + LF+LP  QKMK L+    T+TGY +A  
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKYYEKLMWHEGFSIIGSPVDDFKKLWPSDYQRFW-------------------LIFKFL 180
           SKY++KLMWHEGF++ GSPVDD KKLWP+DY+RF                    LIFKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 GIFDEEMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKY 240
            I +EEM+KLL+  D +  KP+M  RLNSYPPCPDPS+V+GLA HTDTSL T++HQAR  
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQIFNEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFY 300
           GLQ+F +  GW+PL+  S TLI+N+GD+L I+SNGRF SI HRVMI E +E   S+A+FY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 YPPSYLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
            PP  L +SPYCKPLSE+P  P+Y+ VNVKEYF IKA   G+G++A+ I
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of CmoCh05G006840 vs. TrEMBL
Match: E1AXG4_9ROSI (Gibberellin 3-oxidase 1 (Fragment) OS=Marah macrocarpus PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 1.7e-101
Identity = 189/353 (53.54%), Postives = 252/353 (71.39%), Query Frame = 1


Query: 1   MADQKL---PPASLVVPLDFDSV-QTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDN 60
           MADQ++   PP+S +VPLDF S  +T+PESH+W    E S  ++ D+ +S+PV+DL DD 
Sbjct: 1   MADQEITTAPPSSPLVPLDFSSSHETVPESHIWVDSIELSPAMDLDEKLSLPVIDLLDDT 60

Query: 61  VL-ELIGKACKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYC 120
              ELIGKAC++WGMFQLINHG+PK++IAETE+ A+ LFALP +QKMK    PG +TGY 
Sbjct: 61  TASELIGKACQQWGMFQLINHGVPKSIIAETEDEARRLFALPTTQKMKTFG-PG-NTGYG 120

Query: 121 IARFSKYYEKLMWHEGFSIIGSPVDDFKKLWPSDYQRFW-------------------LI 180
           +   SKY+ K MWHEGF+I GSP+DD KKLWPSDY+RF                    LI
Sbjct: 121 MVPLSKYHSKSMWHEGFTIFGSPLDDAKKLWPSDYKRFCDVMEEYQRKMKGLADRLMRLI 180

Query: 181 FKFLGIFDEEMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQ 240
            KFL I +EE++KL+ +    + K +   RLN YPPCPDP +V+G+A+HTDTS  T++HQ
Sbjct: 181 LKFLDISEEEIMKLM-FTPEDSSKIYTALRLNLYPPCPDPDRVVGMASHTDTSFFTIIHQ 240

Query: 241 ARKYGLQIFNEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSM 300
           AR  GLQIF ++ GW+PL+  S TL++N+GDLLQI+SNGRF SI HRVMIQE  E  +S+
Sbjct: 241 ARNDGLQIFKDEAGWVPLSPTSGTLMVNVGDLLQILSNGRFPSILHRVMIQEKMEDRLSL 300

Query: 301 AFFYYPPSYLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
           A+FY PP ++ ++PYCKPLSE+P  P+Y+ V VKEY   K+NN  +G++ + I
Sbjct: 301 AYFYTPPPHIYIAPYCKPLSESPQIPLYRCVTVKEYSTSKSNNNFKGLSTVKI 350

[Close]

BLAST of CmoCh05G006840 vs. TrEMBL
Match: B9IKS1_POPTR (Gibberellin 3 beta-hydroxylase family protein OS=Populus trichocarpa GN=POPTR_0018s01170g PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.3e-80
Identity = 153/338 (45.27%), Postives = 229/338 (67.75%), Query Frame = 1


Query: 12  VVPLDFDSVQTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDNVLELIGKACKEWGMF 71
           ++PLDFDSV+T+P+SH+WP    +S   ESD  +S+P VDL D + ++L+G AC+ WG+F
Sbjct: 18  IIPLDFDSVRTVPDSHVWP----TSHAFESDDQLSIPTVDLMDPDAVKLVGHACETWGVF 77

Query: 72  QLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWHEG 131
           Q+INHGIP  +I E E  A+ LF+LP   K+KAL+ PG +TGY +AR S ++ K MWHEG
Sbjct: 78  QVINHGIPLDIIDEVESEARRLFSLPTGHKLKALRSPGGATGYGLARISPFFSKKMWHEG 137

Query: 132 FSIIGSPVDDFKKLWPSDYQRF-------------------WLIFKFLGIFDEEMVKLLS 191
           F+++GSPVD  ++LWP+DYQRF                    LI K L + +EE+ K++S
Sbjct: 138 FTVMGSPVDHARELWPNDYQRFCDVMEDYQKKMKELAITLMHLILKSLDLSEEEISKVVS 197

Query: 192 YIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIFNEKEGWI 251
               +T       +LNSYP CPDPS+V+GLA HTDTSLLT+L+Q+   GL+IF +  GW+
Sbjct: 198 PGGAST-----ALQLNSYPFCPDPSRVMGLAPHTDTSLLTILYQSTINGLEIFKDGVGWV 257

Query: 252 PLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSYLNVSPYC 311
            ++  + +L++N+GDLL I+SN +F S+ HRV+++E ++  +S+A+FY PP+  +VS   
Sbjct: 258 LVSPTNGSLVVNVGDLLHILSNAQFPSVLHRVVLKEKQQ-RLSLAYFYSPPTDFDVS--- 317

Query: 312 KPLSETP-HTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
            PL+  P   P+Y+ V+V+EY  IKA N+ + ++ + I
Sbjct: 318 -PLALNPAQIPLYRSVSVREYIHIKAKNVEKALSLIRI 341

[Close]

BLAST of CmoCh05G006840 vs. TAIR10
Match: AT1G80340.1 (AT1G80340.1 gibberellin 3-oxidase 2)

HSP 1 Score: 224.9 bits (572), Expect = 7.1e-59
Identity = 117/335 (34.93%), Postives = 191/335 (57.01%), Query Frame = 1


Query: 14  PLDFDSVQTIPESHLW-PHPDESSQKIESDKIVSVPVVDLNDDNVLELIGKACKEWGMFQ 73
           P DF S   +P+S+ W P  D       SD+  ++P++DL+D +V  L+G AC  WG FQ
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWHEGF 133
           + NHG+P  L+ + E +   LF LP  +K+KA +     +GY +AR + ++ K MW EGF
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKMWSEGF 140

Query: 134 SIIGSPVDDFKKLWPSDYQRFWLIFKFLGIFDEEMVKL------------------LSYI 193
           ++IGSP+ DF+KLWPS + ++  I +    ++E M KL                  + + 
Sbjct: 141 TVIGSPLHDFRKLWPSHHLKYCEIIE---EYEEHMQKLAAKLMWFALGSLGVEEKDIQWA 200

Query: 194 DPTT--EKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIFNEKEGWI 253
            P +  +    + +LN YP CP+P + +GLA HTD++L+T+L+Q    GLQ+F +  GW+
Sbjct: 201 GPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWV 260

Query: 254 PLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSYLNVSPYC 313
                  +L++N+GDLL I++NG F S+ HR  +     +  SMA+ + PPS + +SP  
Sbjct: 261 TAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV-RSRFSMAYLWGPPSDIMISPLP 320

Query: 314 KPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAAL 328
           K L +   +P+Y  +  K+Y A KA +  + ++ +
Sbjct: 321 K-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSII 345

[Close]

BLAST of CmoCh05G006840 vs. TAIR10
Match: AT1G15550.1 (AT1G15550.1 gibberellin 3-oxidase 1)

HSP 1 Score: 223.0 bits (567), Expect = 2.7e-58
Identity = 111/334 (33.23%), Postives = 185/334 (55.39%), Query Frame = 1


Query: 16  DFDSVQTIPESHLWPHPDE---SSQKIESDKIVSVPVVDLNDDNVLELIGKACKEWGMFQ 75
           DF S++ +P+S+ W   D+   S+         ++P++DL+  +    IG AC+ WG FQ
Sbjct: 23  DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHACRTWGAFQ 82

Query: 76  LINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWHEGF 135
           + NHG+P  L+ + E +   LF LP  +K+K+ +     +GY +AR + ++ K MW EGF
Sbjct: 83  ISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFNKQMWSEGF 142

Query: 136 SIIGSPVDDFKKLWPSDYQRF-------------------WLIFKFLGIFDEEMVKLLSY 195
           +I GSP++DF+KLWP  +  +                   WL    LG+ +E++    + 
Sbjct: 143 TITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEEDIE--WAS 202

Query: 196 IDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIFNEKEGWIP 255
           +           +LN YP CP+P + +GLA HTD++LLT+L+Q    GLQ+F +  GW+ 
Sbjct: 203 LSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVT 262

Query: 256 LARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSYLNVSPYCK 315
           +     +L++N+GDL  I+SNG F S+ HR  + +T    +S+AF + P S + +SP  K
Sbjct: 263 VPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK 322

Query: 316 PLSETPHTPIYKGVNVKEYFAIKANNLGEGIAAL 328
            +S    +P+Y+ V  KEY   KA +  + ++ +
Sbjct: 323 LVSPV-ESPLYQSVTWKEYLRTKATHFNKALSMI 352

[Close]

BLAST of CmoCh05G006840 vs. TAIR10
Match: AT4G21690.1 (AT4G21690.1 gibberellin 3-oxidase 3)

HSP 1 Score: 209.5 bits (532), Expect = 3.1e-54
Identity = 119/339 (35.10%), Postives = 189/339 (55.75%), Query Frame = 1


Query: 12  VVPLDFDSVQTIPESHLW--PHPDESSQKIESDKIVSVPVVDLNDDNVLELIGKACKEWG 71
           ++PLDF + +T+P+SH+W  P P+ +S  I        PV+ L++     L+ +AC+EWG
Sbjct: 18  IIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEWG 77

Query: 72  MFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWH 131
           +F + +HG+  +L+   +   + LF+LP  +K+ A++ P  STGY + R S +Y+KLMW 
Sbjct: 78  VFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWS 137

Query: 132 EGFSIIGSPVDDFKK-LWPSDYQRFWLIF-KFLGIFDEEMVKLLSYI------------- 191
           EGFS++GS +      LWP D+  F  +  ++    D+   +L+S +             
Sbjct: 138 EGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLGW 197

Query: 192 ---DPT---TEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIFNEK 251
              D T   T+      +LNSYP CPDP   +GLA HTD+SLLT+L+Q    GL+I + +
Sbjct: 198 LVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQ 257

Query: 252 E---GWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSY 311
           E    WI +     +L++ +GDL  IISNG+F S  HR ++ +T    +S A+F  PP  
Sbjct: 258 EEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH-RVSAAYFAGPPKN 317

Query: 312 LNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGI 325
           L + P     S+  H PIY+ +  +EY A KA +  + +
Sbjct: 318 LQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKAL 344

[Close]

BLAST of CmoCh05G006840 vs. TAIR10
Match: AT1G80330.1 (AT1G80330.1 gibberellin 3-oxidase 4)

HSP 1 Score: 186.8 bits (473), Expect = 2.1e-47
Identity = 109/341 (31.96%), Postives = 175/341 (51.32%), Query Frame = 1


Query: 19  SVQTIPESHLWPHPDESSQKIESDKIV------SVPVVDLNDDNVLELIGKACKEWGMFQ 78
           S+QT+PES  W      S    S  +       S+PV+DL++ +V  LIG A K WG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 79  LINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYEKLMWHEGF 138
           + NHGI + L+ + E +++ LF +P  +K++A       +GY   R S ++EK MW EGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 139 SI-----------------------IGSPVDDFKKLWPSDYQRFWLIFKFLGIFDEEMVK 198
           +I                       I   VD+ +KL     +  + I   LG+  E++  
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLAS---RLLYCILGSLGVTVEDIEW 194

Query: 199 LLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIFNEKE 258
                   ++      RLN YP CP+P + +GLA HTD+++LT+LHQ+   GLQ+F E+ 
Sbjct: 195 AHKLEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREES 254

Query: 259 GWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFY-YPPSYLNV 318
           GW+ +      L++NIGDL  I+SNG+  S+ HR  +  T  + +S+A+ +  P   + +
Sbjct: 255 GWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHT-RSRISIAYLWGGPAGDVQI 314

Query: 319 SPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
           +P  K L+      +Y+ +  KEY  IK     + + A+ +
Sbjct: 315 APISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of CmoCh05G006840 vs. TAIR10
Match: AT3G21420.1 (AT3G21420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 153.3 bits (386), Expect = 2.6e-37
Identity = 99/305 (32.46%), Postives = 156/305 (51.15%), Query Frame = 1


Query: 47  VPVVDL-------NDDNVLELI--GKACKEWGMFQLINHGIPKTLIAETEEVAQCLFALP 106
           +PV+DL       NDD   E++   +AC++WG FQ+INHGI   ++ + EEVA   F +P
Sbjct: 55  IPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMP 114

Query: 107 KSQKMKALQVPGTSTGYCIARFSKYYEKLMWHEGFSI-IGSPVDDFKKLWPSDYQRFWLI 166
             +K K    PGT  GY  A      +KL W   F++ +  P     KLWPS   RF   
Sbjct: 115 LEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSES 174

Query: 167 FKFLGIFDEEMVK-LLSYIDPT----TEKPHMLF-------RLNSYPPCPDPSKVIGLAT 226
            +       E+ K LL YI  +     E+   +F       R+N YPPC  P  V+GL+ 
Sbjct: 175 LEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSP 234

Query: 227 HTDTSLLTMLHQARK--YGLQIFNEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPH 286
           H+D S LT+L Q++    GLQI  +   W+P+    + L+INIGD ++++SNG++ S+ H
Sbjct: 235 HSDGSALTVLQQSKNSCVGLQILKDNT-WVPVKPLPNALVINIGDTIEVLSNGKYKSVEH 294

Query: 287 RVMIQETEETTMSMAFFYYPPSYLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGE 328
           R  +   E+  +++  FY P   + + P  + + +  +   Y+  N  +Y     +N  +
Sbjct: 295 RA-VTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDYSYHYVSNKLQ 354

[Close]

BLAST of CmoCh05G006840 vs. NCBI nr
Match: gi|2289032|gb|AAB64347.1| (gibberellin 2beta,3beta-hydroxylase [Cucurbita maxima])

HSP 1 Score: 531.6 bits (1368), Expect = 1.0e-147
Identity = 251/344 (72.97%), Postives = 286/344 (83.14%), Query Frame = 1


Query: 5   KLPPASLVVPLDFDSVQTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDNVLELIGKA 64
           KLP   L+ PLD ++V+ +PESH+WPH DESSQK +SDK VS+PVVD NDDNVLELIGKA
Sbjct: 3   KLPAVGLLTPLDLNTVEAVPESHVWPHLDESSQKFKSDKTVSIPVVDFNDDNVLELIGKA 62

Query: 65  CKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFSKYYE 124
           C+EWGMFQLINHGIPKTL AETEEVA+CLFALPKSQKMK L VPGT+ GYC+AR +K+++
Sbjct: 63  CEEWGMFQLINHGIPKTLTAETEEVARCLFALPKSQKMKTLNVPGTANGYCMARLTKHHD 122

Query: 125 KLMWHEGFSIIGSPVDDFKKLWPSDYQRFW-------------------LIFKFLGIFDE 184
           K+MWHEGF++IGSPVDDFKKLWPSDYQ F                    LIFK+LGI DE
Sbjct: 123 KMMWHEGFTVIGSPVDDFKKLWPSDYQPFCDKVEEYQLKMKALADKLISLIFKYLGISDE 182

Query: 185 EMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYGLQIF 244
           EMVK LSYIDP T KPH+  RLNS+PPCP+PSKV+GLA HTDTSL TMLHQAR+ GLQI 
Sbjct: 183 EMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVMGLAAHTDTSLFTMLHQARREGLQIL 242

Query: 245 NEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYYPPSY 304
           NEKEGW+PLA R D LIINIGD LQIISNGRFHS+PHRVMI+ETE+TTMSMA+F++PP +
Sbjct: 243 NEKEGWLPLAPRRDALIINIGDFLQIISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPGH 302

Query: 305 LNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
           L V+PYCKPLSET  TPIYKGVNVKEYF IKA   G+GIAALTI
Sbjct: 303 LYVAPYCKPLSETLQTPIYKGVNVKEYFIIKAKASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006840 vs. NCBI nr
Match: gi|568384792|ref|NP_001275523.1| (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus])

HSP 1 Score: 431.0 bits (1107), Expect = 1.8e-117
Identity = 209/350 (59.71%), Postives = 257/350 (73.43%), Query Frame = 1


Query: 1   MADQKLPPASLVVPLDFDSVQTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDNVLEL 60
           MADQ++PP S +VPLDF SVQT+PESHLWP+  E S  I+ DK VS+P++DL  D   EL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  IGKACKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPG--TSTGYCIAR 120
           +G+AC+EWGMFQLINHGI KTLI E EE  + LF LP +QKMK L+ PG  T+TGY +A 
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 FSKYYEKLMWHEGFSIIGSPVDDFKKLWPSDYQRFW-------------------LIFKF 180
            SKY+ KLMWHEGF+I+GSP+++ KKLWP+DY+RF                    LI KF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LGIFDEEMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARK 240
           LGI +EEM+KLL+  D +  KPHM  RLNSYPPCPDP +V+GLA HTDTSL T+LHQ   
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 YGLQIFNEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFF 300
            GLQIF +  GW+PL+  S TLI+NIGDLL I+SNGRF SI HRVMIQE +E   S+A+F
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YYPPSYLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
           Y PP  + +SPYCKPLS+TP  P+Y+ VNVKEYFAIKA   G+G+ A+ I
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of CmoCh05G006840 vs. NCBI nr
Match: gi|8894936|gb|AAF80661.1|AF074710_1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 426.4 bits (1095), Expect = 4.5e-116
Identity = 203/349 (58.17%), Postives = 259/349 (74.21%), Query Frame = 1


Query: 1   MADQKLPPASLVVPLDFDSVQTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDNVLEL 60
           MADQ+LPP S +VPLDF+S+QT+PESHLWP+  E S  I+ D+ +S+P++D  DD   EL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  IGKACKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPG-TSTGYCIARF 120
           +G+AC++WGMFQLINHG+PKTLI E EE  + LF+LP  QKMK L+    T+TGY +A  
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKYYEKLMWHEGFSIIGSPVDDFKKLWPSDYQRFW-------------------LIFKFL 180
           SKY++KLMWHEGF++ GSPVDD KKLWP+DY+RF                    LIFKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 GIFDEEMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKY 240
            I +EEM+KLL+  D +  KP+M  RLNSYPPCPDPS+V+GLA HTDTSL T++HQAR  
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQIFNEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFY 300
           GLQ+F +  GW+PL+  S TLI+N+GD+L I+SNGRF SI HRVMI E +E   S+A+FY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 YPPSYLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
            PP  L +SPYCKPLSE+P  P+Y+ VNVKEYF IKA   G+G++A+ I
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of CmoCh05G006840 vs. NCBI nr
Match: gi|659088742|ref|XP_008445138.1| (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 422.9 bits (1086), Expect = 5.0e-115
Identity = 204/351 (58.12%), Postives = 260/351 (74.07%), Query Frame = 1


Query: 1   MADQKLPPASLVVPLDFDSVQT-IPESHLWPHPDESSQKIESDKIVSVPVVDLNDDNVLE 60
           MADQ++PP S +VPL+F SVQT +PESHLWP+  E S  ++ DK VS+P++DL +D+  E
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LIGKACKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPG--TSTGYCIA 120
           L+G+AC+EWGMFQLINHG+ +TLI E EE  + LF LP +QKMK L+ PG  T+TGY +A
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 RFSKYYEKLMWHEGFSIIGSPVDDFKKLWPSDYQRFW-------------------LIFK 180
             SKY+ KLMWHEGF+IIGSP+++ KKLWP+DY+RF                    LI K
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLGIFDEEMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQAR 240
           FLGI +EEM+KLL+  D +  KP+M  RLNSYPPCPDP +V+GLA HTDTSL T+LHQ R
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 KYGLQIFNEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAF 300
             GLQIF +  GW+PL+  S TL++N+GDLL I+SNGRF SI HRVMIQE +E   S+A+
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYYPPSYLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
           FY PP  + +SPYCKPLS+TP  P+Y+ VNVKEYFAIKA  +G G+ A+ I
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

[Close]

BLAST of CmoCh05G006840 vs. NCBI nr
Match: gi|304636271|gb|ADM47337.1| (gibberellin 3-oxidase 1 [Marah macrocarpus])

HSP 1 Score: 377.5 bits (968), Expect = 2.4e-101
Identity = 189/353 (53.54%), Postives = 252/353 (71.39%), Query Frame = 1


Query: 1   MADQKL---PPASLVVPLDFDSV-QTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDN 60
           MADQ++   PP+S +VPLDF S  +T+PESH+W    E S  ++ D+ +S+PV+DL DD 
Sbjct: 1   MADQEITTAPPSSPLVPLDFSSSHETVPESHIWVDSIELSPAMDLDEKLSLPVIDLLDDT 60

Query: 61  VL-ELIGKACKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYC 120
              ELIGKAC++WGMFQLINHG+PK++IAETE+ A+ LFALP +QKMK    PG +TGY 
Sbjct: 61  TASELIGKACQQWGMFQLINHGVPKSIIAETEDEARRLFALPTTQKMKTFG-PG-NTGYG 120

Query: 121 IARFSKYYEKLMWHEGFSIIGSPVDDFKKLWPSDYQRFW-------------------LI 180
           +   SKY+ K MWHEGF+I GSP+DD KKLWPSDY+RF                    LI
Sbjct: 121 MVPLSKYHSKSMWHEGFTIFGSPLDDAKKLWPSDYKRFCDVMEEYQRKMKGLADRLMRLI 180

Query: 181 FKFLGIFDEEMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQ 240
            KFL I +EE++KL+ +    + K +   RLN YPPCPDP +V+G+A+HTDTS  T++HQ
Sbjct: 181 LKFLDISEEEIMKLM-FTPEDSSKIYTALRLNLYPPCPDPDRVVGMASHTDTSFFTIIHQ 240

Query: 241 ARKYGLQIFNEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSM 300
           AR  GLQIF ++ GW+PL+  S TL++N+GDLLQI+SNGRF SI HRVMIQE  E  +S+
Sbjct: 241 ARNDGLQIFKDEAGWVPLSPTSGTLMVNVGDLLQILSNGRFPSILHRVMIQEKMEDRLSL 300

Query: 301 AFFYYPPSYLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 330
           A+FY PP ++ ++PYCKPLSE+P  P+Y+ V VKEY   K+NN  +G++ + I
Sbjct: 301 AYFYTPPPHIYIAPYCKPLSESPQIPLYRCVTVKEYSTSKSNNNFKGLSTVKI 350

The following BLAST results are available for this feature:

BLAST of CmoCh05G006840 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G3OX_PEA	8.4e-70	39.94	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1	[more]
G3OX2_ARATH	1.3e-57	34.93	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana GN=GA3OX2 PE=1 SV=2	[more]
G3OX1_ARATH	4.8e-57	33.23	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2	[more]
G3OX3_ARATH	5.4e-53	35.10	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana GN=GA3OX3 PE=2 SV=1	[more]
G3O23_WHEAT	3.0e-51	34.11	Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum GN=GA3ox2-3 PE=2 SV=1	[more]

BLAST of CmoCh05G006840 vs. TrEMBL


Match Name	E-value	Identity	Description
O24417_CUCMA	7.0e-148	72.97	Gibberellin 2beta,3beta-hydroxylase OS=Cucurbita maxima PE=2 SV=1	[more]
S0BE47_CUCSA	1.3e-117	59.71	GA 3-oxidase OS=Cucumis sativus GN=GA3ox4 PE=2 SV=1	[more]
Q9LM06_CITLA	3.2e-116	58.17	Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus GN=WGA3H PE=2 SV=1	[more]
E1AXG4_9ROSI	1.7e-101	53.54	Gibberellin 3-oxidase 1 (Fragment) OS=Marah macrocarpus PE=2 SV=1	[more]
B9IKS1_POPTR	1.3e-80	45.27	Gibberellin 3 beta-hydroxylase family protein OS=Populus trichocarpa GN=POPTR_00...	[more]

BLAST of CmoCh05G006840 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G80340.1	7.1e-59	34.93	gibberellin 3-oxidase 2	[more]
AT1G15550.1	2.7e-58	33.23	gibberellin 3-oxidase 1	[more]
AT4G21690.1	3.1e-54	35.10	gibberellin 3-oxidase 3	[more]
AT1G80330.1	2.1e-47	31.96	gibberellin 3-oxidase 4	[more]
AT3G21420.1	2.6e-37	32.46	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of CmoCh05G006840 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|2289032\|gb\|AAB64347.1\|	1.0e-147	72.97	gibberellin 2beta,3beta-hydroxylase [Cucurbita maxima]	[more]
gi\|568384792\|ref\|NP_001275523.1\|	1.8e-117	59.71	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus]	[more]
gi\|8894936\|gb\|AAF80661.1\|AF074710_1	4.5e-116	58.17	putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris]	[more]
gi\|659088742\|ref\|XP_008445138.1\|	5.0e-115	58.12	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]
gi\|304636271\|gb\|ADM47337.1\|	2.4e-101	53.54	gibberellin 3-oxidase 1 [Marah macrocarpus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR026992	DIOX_N
IPR027443	IPNS-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh05G006840.1	CmoCh05G006840.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 182..280 score: 1.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 180..282 score: 10
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 47..148 score: 4.6
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 19..319 score: 1.1
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..326 score: 1.3E
None	No IPR available	PANTHER	PTHR10209:SF197	GIBBERELLIN 3-BETA-DIOXYGENASE 3	coord: 1..326 score: 1.3E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 12..321 score: 1.72

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh05G006840	Cucumber (Gy14) v2	cgybcmoB239
CmoCh05G006840	Melon (DHL92) v3.6.1	cmomedB816
CmoCh05G006840	Silver-seed gourd	carcmoB0087
CmoCh05G006840	Silver-seed gourd	carcmoB1318
CmoCh05G006840	Silver-seed gourd	carcmoB1320
CmoCh05G006840	Cucumber (Chinese Long) v3	cmocucB0919
CmoCh05G006840	Watermelon (97103) v2	cmowmbB800
CmoCh05G006840	Wax gourd	cmowgoB0947
CmoCh05G006840	Cucurbita moschata (Rifu)	cmocmoB151
CmoCh05G006840	Cucurbita moschata (Rifu)	cmocmoB159
CmoCh05G006840	Cucurbita moschata (Rifu)	cmocmoB373
CmoCh05G006840	Cucumber (Gy14) v1	cgycmoB0461
CmoCh05G006840	Cucurbita maxima (Rimu)	cmacmoB194
CmoCh05G006840	Cucurbita maxima (Rimu)	cmacmoB486
CmoCh05G006840	Cucurbita maxima (Rimu)	cmacmoB786
CmoCh05G006840	Wild cucumber (PI 183967)	cmocpiB783
CmoCh05G006840	Cucumber (Chinese Long) v2	cmocuB778
CmoCh05G006840	Melon (DHL92) v3.5.1	cmomeB719
CmoCh05G006840	Watermelon (Charleston Gray)	cmowcgB713
CmoCh05G006840	Watermelon (97103) v1	cmowmB746
CmoCh05G006840	Cucurbita pepo (Zucchini)	cmocpeB720
CmoCh05G006840	Cucurbita pepo (Zucchini)	cmocpeB747
CmoCh05G006840	Cucurbita pepo (Zucchini)	cmocpeB754
CmoCh05G006840	Bottle gourd (USVL1VR-Ls)	cmolsiB728