CmoCh05G006810 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh05G006810
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase
Location	Cmo_Chr05 : 3455838 .. 3456416 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAATACCAACTCAAAATGAAGGCTTTAGCTGATAAGCTAATAAGCTTAATCTTCAAGTTTCTTGGTATCTCCGATGAAGAGATGGTGAAGAAGTTGTCTTACATCGACCCGGCCACCGGAAAACCCCACTTGGCTTTGCGCTTGAACTCGTTTCCTCCATGCCCTGAACCAAGCAAAGTCATTGGCCTCGCGGCCCACACTGACACCTCCCTCTTCACCATGCTTCACCAGGCACGCAGAGAGGGGTTACAGATCTTGAACGAGAAAGATGGCTGGCTTCCGCTGGCTCCGAGGAGCGATGCTCTCATTATTAACGTCGGCGATTTCCTCCAAATTATATCGAACGGGCGGTTTCATAGCGTTCCTCACCGGGTGATGATACGAGAGACTGAGAAGACTACGATGTCGATGGCATATTTCTTTCATCCACCAGGTCATTTATACGTAGCACCTTATTGTAAGCCATTGAGTGAAACTCTACAGACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCATCATCAAGGCTAAAGCCTCGGGGAAGGGAATTGCTGCCCTAACAATATGA

mRNA sequence

ATGGAAGAATACCAACTCAAAATGAAGGCTTTAGCTGATAAGCTAATAAGCTTAATCTTCAAGTTTCTTGGTATCTCCGATGAAGAGATGGTGAAGAAGTTGTCTTACATCGACCCGGCCACCGGAAAACCCCACTTGGCTTTGCGCTTGAACTCGTTTCCTCCATGCCCTGAACCAAGCAAAGTCATTGGCCTCGCGGCCCACACTGACACCTCCCTCTTCACCATGCTTCACCAGGCACGCAGAGAGGGGTTACAGATCTTGAACGAGAAAGATGGCTGGCTTCCGCTGGCTCCGAGGAGCGATGCTCTCATTATTAACGTCGGCGATTTCCTCCAAATTATATCGAACGGGCGGTTTCATAGCGTTCCTCACCGGGTGATGATACGAGAGACTGAGAAGACTACGATGTCGATGGCATATTTCTTTCATCCACCAGGTCATTTATACGTAGCACCTTATTGTAAGCCATTGAGTGAAACTCTACAGACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCATCATCAAGGCTAAAGCCTCGGGGAAGGGAATTGCTGCCCTAACAATATGA

Coding sequence (CDS)

ATGGAAGAATACCAACTCAAAATGAAGGCTTTAGCTGATAAGCTAATAAGCTTAATCTTCAAGTTTCTTGGTATCTCCGATGAAGAGATGGTGAAGAAGTTGTCTTACATCGACCCGGCCACCGGAAAACCCCACTTGGCTTTGCGCTTGAACTCGTTTCCTCCATGCCCTGAACCAAGCAAAGTCATTGGCCTCGCGGCCCACACTGACACCTCCCTCTTCACCATGCTTCACCAGGCACGCAGAGAGGGGTTACAGATCTTGAACGAGAAAGATGGCTGGCTTCCGCTGGCTCCGAGGAGCGATGCTCTCATTATTAACGTCGGCGATTTCCTCCAAATTATATCGAACGGGCGGTTTCATAGCGTTCCTCACCGGGTGATGATACGAGAGACTGAGAAGACTACGATGTCGATGGCATATTTCTTTCATCCACCAGGTCATTTATACGTAGCACCTTATTGTAAGCCATTGAGTGAAACTCTACAGACTCCCATTTATAAAGGAGTGAACGTGAAAGAATATTTCATCATCAAGGCTAAAGCCTCGGGGAAGGGAATTGCTGCCCTAACAATATGA

Homology

[Close]

BLAST of CmoCh05G006810 vs. Swiss-Prot
Match: G3OX1_ARATH (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 136.3 bits (342), Expect = 3.4e-31
Identity = 70/190 (36.84%), Postives = 114/190 (60.00%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           +EEY+  MK LA KL+ L    LG+S+E++  + + +         AL+LN +P CPEP 
Sbjct: 167 VEEYEEHMKKLASKLMWLALNSLGVSEEDI--EWASLSSDLNWAQAALQLNHYPVCPEPD 226

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           + +GLAAHTD++L T+L+Q    GLQ+  +  GW+ + P   +L++NVGD   I+SNG F
Sbjct: 227 RAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLF 286

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            SV HR  + +T +  +S+A+ + P   + ++P  K L   +++P+Y+ V  KEY   KA
Sbjct: 287 KSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK-LVSPVESPLYQSVTWKEYLRTKA 346

Query: 181 KASGKGIAAL 191
               K ++ +
Sbjct: 347 THFNKALSMI 352

[Close]

BLAST of CmoCh05G006810 vs. Swiss-Prot
Match: G3OX4_ARATH (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.2e-30
Identity = 71/195 (36.41%), Postives = 115/195 (58.97%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGIS--DEEMVKKLSYIDPATGKPHLALRLNSFPPCPE 60
           ++EY  +M+ LA +L+  I   LG++  D E   KL       G+   A+RLN +P CPE
Sbjct: 160 IQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRG--AIRLNHYPVCPE 219

Query: 61  PSKVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNG 120
           P + +GLAAHTD+++ T+LHQ+   GLQ+  E+ GW+ + P    L++N+GD   I+SNG
Sbjct: 220 PERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNG 279

Query: 121 RFHSVPHRVMIRETEKTTMSMAYFF-HPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFI 180
           +  SV HR  +  T ++ +S+AY +  P G + +AP  K L+   +  +Y+ +  KEY  
Sbjct: 280 KIPSVVHRAKVNHT-RSRISIAYLWGGPAGDVQIAPISK-LTGPAEPSLYRSITWKEYLQ 339

Query: 181 IKAKASGKGIAALTI 193
           IK +   K + A+ +
Sbjct: 340 IKYEVFDKAMDAIRV 350

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BLAST of CmoCh05G006810 vs. Swiss-Prot
Match: G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 4.9e-30
Identity = 70/190 (36.84%), Postives = 109/190 (57.37%), Query Frame = 1


Query: 3   EYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKV 62
           +Y   MK LA  L+ L+   LGI+ E++  K +       K   AL+LNS+P CP+P   
Sbjct: 168 QYDETMKKLAGTLMCLMLDSLGITKEDI--KWAGSKAQFEKACAALQLNSYPSCPDPDHA 227

Query: 63  IGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRFHS 122
           +GLA HTD++  T+L Q    GLQ+  E  GW+ + P    L++NVGD   I+SNG + S
Sbjct: 228 MGLAPHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGLYPS 287

Query: 123 VPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKAKA 182
           V HRV++  T +   S+AY + PP ++ + P+ K +  T + P+Y+ V   EY   KAK 
Sbjct: 288 VLHRVLVNRT-RQRFSVAYLYGPPSNVEICPHAKLIGPT-KPPLYRSVTWNEYLGTKAKH 347

Query: 183 SGKGIAALTI 193
             K ++++ +
Sbjct: 348 FNKALSSVRL 353

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BLAST of CmoCh05G006810 vs. Swiss-Prot
Match: G3O23_WHEAT (Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum GN=GA3ox2-3 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.4e-30
Identity = 74/196 (37.76%), Postives = 112/196 (57.14%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMV-----KKLSYIDPATGKPHLALRLNSFPP 60
           MEE+  +M+ALADKL+ L    LG++ E++      +K++    AT      + LN +P 
Sbjct: 163 MEEFHREMRALADKLLELFLVALGLTGEQVAAVESEQKIAETMTAT------MHLNWYPK 222

Query: 61  CPEPSKVIGLAAHTDTSLFTMLHQARREGLQILNE-KDGWLPLAPRSDALIINVGDFLQI 120
           CP+P + +GL AHTD+  FT + Q+   GLQ+     D W+ +     A+++NVGD  QI
Sbjct: 223 CPDPKRALGLIAHTDSGFFTFVLQSLVPGLQLFRHGPDRWVTVPAVPGAMVVNVGDLFQI 282

Query: 121 ISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKE 180
           ++NGRFHSV HR ++   E   +S+ YF  PP H+ VAP  + L+ T     Y+ V   E
Sbjct: 283 LTNGRFHSVYHRAVVNR-ESDRISLGYFLGPPAHVKVAPLREALAGT--PAAYRAVTWPE 342

Query: 181 YFIIKAKASGKGIAAL 191
           Y  ++ KA   G +AL
Sbjct: 343 YMGVRKKAFTTGASAL 349

[Close]

BLAST of CmoCh05G006810 vs. Swiss-Prot
Match: G3O22_WHEAT (Gibberellin 3-beta-dioxygenase 2-2 OS=Triticum aestivum GN=GA3ox2-2 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.4e-29
Identity = 73/196 (37.24%), Postives = 112/196 (57.14%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMV-----KKLSYIDPATGKPHLALRLNSFPP 60
           MEE+  +M+ALADKL+ L    LG++ E++      +K++    AT      + LN +P 
Sbjct: 163 MEEFHREMRALADKLLELFLVALGLTGEQVAAVESEQKIAETMTAT------MHLNWYPK 222

Query: 61  CPEPSKVIGLAAHTDTSLFTMLHQARREGLQILNE-KDGWLPLAPRSDALIINVGDFLQI 120
           CP+P + +GL AHTD+  FT + Q+   GLQ+     D W+ +     A+++NVGD  QI
Sbjct: 223 CPDPKRALGLIAHTDSGFFTFVLQSLVPGLQLFRHGPDRWVTVPAVPGAMVVNVGDLFQI 282

Query: 121 ISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKE 180
           ++NGRFHSV HR ++   +   +S+ YF  PP H+ VAP  + L+ T     Y+ V   E
Sbjct: 283 LTNGRFHSVYHRAVVNR-DSDRISLGYFLGPPAHVKVAPLREALAGT--PAAYRAVTWPE 342

Query: 181 YFIIKAKASGKGIAAL 191
           Y  ++ KA   G +AL
Sbjct: 343 YMGVRKKAFTTGASAL 349

[Close]

BLAST of CmoCh05G006810 vs. TrEMBL
Match: O24417_CUCMA (Gibberellin 2beta,3beta-hydroxylase OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 1.4e-103
Identity = 186/192 (96.88%), Postives = 191/192 (99.48%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           +EEYQLKMKALADKLISLIFK+LGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 VEEYQLKMKALADKLISLIFKYLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KV+GLAAHTDTSLFTMLHQARREGLQILNEK+GWLPLAPR DALIIN+GDFLQIISNGRF
Sbjct: 215 KVMGLAAHTDTSLFTMLHQARREGLQILNEKEGWLPLAPRRDALIINIGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. TrEMBL
Match: Q9LM06_CITLA (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus GN=WGA3H PE=2 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 9.3e-68
Identity = 120/192 (62.50%), Postives = 156/192 (81.25%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQ +MK L +++I LIFKFL IS+EEM+K L+  D + GKP++ALRLNS+PPCP+PS
Sbjct: 158 MEEYQSQMKGLGERIIRLIFKFLDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPS 217

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           +V+GLAAHTDTSLFT++HQAR +GLQ+  +  GW+PL+P S  LI+NVGD L I+SNGRF
Sbjct: 218 RVMGLAAHTDTSLFTIVHQARHDGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRF 277

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            S+ HRVMI E ++   S+AYF+ PPG LY++PYCKPLSE+ Q P+Y+ VNVKEYF IKA
Sbjct: 278 PSILHRVMIHENKEHRFSLAYFYDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKA 337

Query: 181 KASGKGIAALTI 193
           K  GKG++A+ I
Sbjct: 338 KKEGKGLSAIKI 349

[Close]

BLAST of CmoCh05G006810 vs. TrEMBL
Match: A0A0A0LSC5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G379320 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 5.1e-66
Identity = 117/192 (60.94%), Postives = 152/192 (79.17%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQ +MK L +++I LI KFLGIS+EEM+K L+  D + GKPH+ALRLNS+PPCP+P 
Sbjct: 1   MEEYQREMKGLGERIIRLILKFLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPG 60

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           +V+GLAAHTDTSL T+LHQ    GLQI  +  GW+PL+P S  LI+N+GD L I+SNGRF
Sbjct: 61  QVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRF 120

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            S+ HRVMI+E ++   S+AYF+ PPG +Y++PYCKPLS+T Q P+Y+ VNVKEYF IKA
Sbjct: 121 PSILHRVMIQENKEHRFSLAYFYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKA 180

Query: 181 KASGKGIAALTI 193
           K +GKG+ A+ I
Sbjct: 181 KKTGKGLPAIKI 192

[Close]

BLAST of CmoCh05G006810 vs. TrEMBL
Match: S0BE47_CUCSA (GA 3-oxidase OS=Cucumis sativus GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 5.1e-66
Identity = 117/192 (60.94%), Postives = 152/192 (79.17%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQ +MK L +++I LI KFLGIS+EEM+K L+  D + GKPH+ALRLNS+PPCP+P 
Sbjct: 159 MEEYQREMKGLGERIIRLILKFLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPG 218

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           +V+GLAAHTDTSL T+LHQ    GLQI  +  GW+PL+P S  LI+N+GD L I+SNGRF
Sbjct: 219 QVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRF 278

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            S+ HRVMI+E ++   S+AYF+ PPG +Y++PYCKPLS+T Q P+Y+ VNVKEYF IKA
Sbjct: 279 PSILHRVMIQENKEHRFSLAYFYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKA 338

Query: 181 KASGKGIAALTI 193
           K +GKG+ A+ I
Sbjct: 339 KKTGKGLPAIKI 350

[Close]

BLAST of CmoCh05G006810 vs. TrEMBL
Match: E1AXG4_9ROSI (Gibberellin 3-oxidase 1 (Fragment) OS=Marah macrocarpus PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 8.7e-58
Identity = 107/192 (55.73%), Postives = 147/192 (76.56%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQ KMK LAD+L+ LI KFL IS+EE++K L +    + K + ALRLN +PPCP+P 
Sbjct: 160 MEEYQRKMKGLADRLMRLILKFLDISEEEIMK-LMFTPEDSSKIYTALRLNLYPPCPDPD 219

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           +V+G+A+HTDTS FT++HQAR +GLQI  ++ GW+PL+P S  L++NVGD LQI+SNGRF
Sbjct: 220 RVVGMASHTDTSFFTIIHQARNDGLQIFKDEAGWVPLSPTSGTLMVNVGDLLQILSNGRF 279

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            S+ HRVMI+E  +  +S+AYF+ PP H+Y+APYCKPLSE+ Q P+Y+ V VKEY   K+
Sbjct: 280 PSILHRVMIQEKMEDRLSLAYFYTPPPHIYIAPYCKPLSESPQIPLYRCVTVKEYSTSKS 339

Query: 181 KASGKGIAALTI 193
             + KG++ + I
Sbjct: 340 NNNFKGLSTVKI 350

[Close]

BLAST of CmoCh05G006810 vs. TAIR10
Match: AT1G15550.1 (AT1G15550.1 gibberellin 3-oxidase 1)

HSP 1 Score: 136.3 bits (342), Expect = 1.9e-32
Identity = 70/190 (36.84%), Postives = 114/190 (60.00%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           +EEY+  MK LA KL+ L    LG+S+E++  + + +         AL+LN +P CPEP 
Sbjct: 167 VEEYEEHMKKLASKLMWLALNSLGVSEEDI--EWASLSSDLNWAQAALQLNHYPVCPEPD 226

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           + +GLAAHTD++L T+L+Q    GLQ+  +  GW+ + P   +L++NVGD   I+SNG F
Sbjct: 227 RAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLF 286

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            SV HR  + +T +  +S+A+ + P   + ++P  K L   +++P+Y+ V  KEY   KA
Sbjct: 287 KSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK-LVSPVESPLYQSVTWKEYLRTKA 346

Query: 181 KASGKGIAAL 191
               K ++ +
Sbjct: 347 THFNKALSMI 352

[Close]

BLAST of CmoCh05G006810 vs. TAIR10
Match: AT1G80330.1 (AT1G80330.1 gibberellin 3-oxidase 4)

HSP 1 Score: 133.7 bits (335), Expect = 1.2e-31
Identity = 71/195 (36.41%), Postives = 115/195 (58.97%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGIS--DEEMVKKLSYIDPATGKPHLALRLNSFPPCPE 60
           ++EY  +M+ LA +L+  I   LG++  D E   KL       G+   A+RLN +P CPE
Sbjct: 160 IQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRG--AIRLNHYPVCPE 219

Query: 61  PSKVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNG 120
           P + +GLAAHTD+++ T+LHQ+   GLQ+  E+ GW+ + P    L++N+GD   I+SNG
Sbjct: 220 PERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNG 279

Query: 121 RFHSVPHRVMIRETEKTTMSMAYFF-HPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFI 180
           +  SV HR  +  T ++ +S+AY +  P G + +AP  K L+   +  +Y+ +  KEY  
Sbjct: 280 KIPSVVHRAKVNHT-RSRISIAYLWGGPAGDVQIAPISK-LTGPAEPSLYRSITWKEYLQ 339

Query: 181 IKAKASGKGIAALTI 193
           IK +   K + A+ +
Sbjct: 340 IKYEVFDKAMDAIRV 350

[Close]

BLAST of CmoCh05G006810 vs. TAIR10
Match: AT1G80340.1 (AT1G80340.1 gibberellin 3-oxidase 2)

HSP 1 Score: 127.5 bits (319), Expect = 8.9e-30
Identity = 67/182 (36.81%), Postives = 105/182 (57.69%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATG--KPHLALRLNSFPPCPE 60
           +EEY+  M+ LA KL+      LG+ +    K + +  P +        ++LN +P CPE
Sbjct: 160 IEEYEEHMQKLAAKLMWFALGSLGVEE----KDIQWAGPNSDFQGTQAVIQLNHYPKCPE 219

Query: 61  PSKVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNG 120
           P + +GLAAHTD++L T+L+Q    GLQ+  +  GW+   P   +L++NVGD L I++NG
Sbjct: 220 PDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNG 279

Query: 121 RFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFII 180
            F SV HR  +    ++  SMAY + PP  + ++P  K L + LQ+P+Y  +  K+Y   
Sbjct: 280 IFPSVLHRARVNHV-RSRFSMAYLWGPPSDIMISPLPK-LVDPLQSPLYPSLTWKQYLAT 335

[Close]

BLAST of CmoCh05G006810 vs. TAIR10
Match: AT4G21690.1 (AT4G21690.1 gibberellin 3-oxidase 3)

HSP 1 Score: 117.5 bits (293), Expect = 9.3e-27
Identity = 71/195 (36.41%), Postives = 106/195 (54.36%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDP-----ATGKPHLALRLNSFPP 60
           MEEYQ  M  L+ +LIS++   LG++ E+    L ++ P      T      L+LNS+P 
Sbjct: 158 MEEYQKAMDDLSHRLISMLMGSLGLTHED----LGWLVPDKTGSGTDSIQSFLQLNSYPV 217

Query: 61  CPEPSKVIGLAAHTDTSLFTMLHQARREGLQI---LNEKDGWLPLAPRSDALIINVGDFL 120
           CP+P   +GLA HTD+SL T+L+Q    GL+I     E   W+ + P   +L++ +GD  
Sbjct: 218 CPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLS 277

Query: 121 QIISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNV 180
            IISNG+F S  HR ++ +T    +S AYF  PP +L + P     S+    PIY+ +  
Sbjct: 278 HIISNGQFRSTMHRAVVNKTHH-RVSAAYFAGPPKNLQIGPL---TSDKNHPPIYRRLIW 337

Query: 181 KEYFIIKAKASGKGI 188
           +EY   KA    K +
Sbjct: 338 EEYLAAKATHFNKAL 344

[Close]

BLAST of CmoCh05G006810 vs. TAIR10
Match: AT1G78550.1 (AT1G78550.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 112.1 bits (279), Expect = 3.9e-25
Identity = 66/189 (34.92%), Postives = 110/189 (58.20%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           +E Y  ++K++A  L + +   L I  EEM      +         ++++N +PPCP+P 
Sbjct: 171 LETYSSEVKSIAKILFAKMASVLEIKHEEMEDLFDDV-------WQSIKINYYPPCPQPD 230

Query: 61  KVIGLAAHTDTSLFTMLHQARR-EGLQILNEKDG-WLPLAPRSDALIINVGDFLQIISNG 120
           +V+GL  H+D +  T+L Q  + EGLQI  +KDG W+ + P  DAL++NVG+ L+II+NG
Sbjct: 231 QVMGLTQHSDAAGLTILLQVNQVEGLQI--KKDGKWVVVKPLRDALVVNVGEILEIITNG 290

Query: 121 RFHSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYF-- 180
           R+ S+ HR ++  +EK  +S+A  FH PG   +    K L +  +  ++K ++ +EYF  
Sbjct: 291 RYRSIEHRAVV-NSEKERLSVA-MFHSPGKETIIRPAKSLVDRQKQCLFKSMSTQEYFDA 348

Query: 181 IIKAKASGK 186
               K +GK
Sbjct: 351 FFTQKLNGK 348

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: gi|2289032|gb|AAB64347.1| (gibberellin 2beta,3beta-hydroxylase [Cucurbita maxima])

HSP 1 Score: 383.6 bits (984), Expect = 2.0e-103
Identity = 186/192 (96.88%), Postives = 191/192 (99.48%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           +EEYQLKMKALADKLISLIFK+LGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS
Sbjct: 155 VEEYQLKMKALADKLISLIFKYLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 214

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           KV+GLAAHTDTSLFTMLHQARREGLQILNEK+GWLPLAPR DALIIN+GDFLQIISNGRF
Sbjct: 215 KVMGLAAHTDTSLFTMLHQARREGLQILNEKEGWLPLAPRRDALIINIGDFLQIISNGRF 274

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
           HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA
Sbjct: 275 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 334

Query: 181 KASGKGIAALTI 193
           KASGKGIAALTI
Sbjct: 335 KASGKGIAALTI 346

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: gi|8894936|gb|AAF80661.1|AF074710_1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 264.6 bits (675), Expect = 1.3e-67
Identity = 120/192 (62.50%), Postives = 156/192 (81.25%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQ +MK L +++I LIFKFL IS+EEM+K L+  D + GKP++ALRLNS+PPCP+PS
Sbjct: 158 MEEYQSQMKGLGERIIRLIFKFLDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPS 217

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           +V+GLAAHTDTSLFT++HQAR +GLQ+  +  GW+PL+P S  LI+NVGD L I+SNGRF
Sbjct: 218 RVMGLAAHTDTSLFTIVHQARHDGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRF 277

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            S+ HRVMI E ++   S+AYF+ PPG LY++PYCKPLSE+ Q P+Y+ VNVKEYF IKA
Sbjct: 278 PSILHRVMIHENKEHRFSLAYFYDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKA 337

Query: 181 KASGKGIAALTI 193
           K  GKG++A+ I
Sbjct: 338 KKEGKGLSAIKI 349

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: gi|568384792|ref|NP_001275523.1| (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus])

HSP 1 Score: 258.8 bits (660), Expect = 7.3e-66
Identity = 117/192 (60.94%), Postives = 152/192 (79.17%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQ +MK L +++I LI KFLGIS+EEM+K L+  D + GKPH+ALRLNS+PPCP+P 
Sbjct: 159 MEEYQREMKGLGERIIRLILKFLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPG 218

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           +V+GLAAHTDTSL T+LHQ    GLQI  +  GW+PL+P S  LI+N+GD L I+SNGRF
Sbjct: 219 QVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRF 278

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            S+ HRVMI+E ++   S+AYF+ PPG +Y++PYCKPLS+T Q P+Y+ VNVKEYF IKA
Sbjct: 279 PSILHRVMIQENKEHRFSLAYFYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKA 338

Query: 181 KASGKGIAALTI 193
           K +GKG+ A+ I
Sbjct: 339 KKTGKGLPAIKI 350

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: gi|700207782|gb|KGN62901.1| (hypothetical protein Csa_2G379320 [Cucumis sativus])

HSP 1 Score: 258.8 bits (660), Expect = 7.3e-66
Identity = 117/192 (60.94%), Postives = 152/192 (79.17%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQ +MK L +++I LI KFLGIS+EEM+K L+  D + GKPH+ALRLNS+PPCP+P 
Sbjct: 1   MEEYQREMKGLGERIIRLILKFLGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPG 60

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           +V+GLAAHTDTSL T+LHQ    GLQI  +  GW+PL+P S  LI+N+GD L I+SNGRF
Sbjct: 61  QVMGLAAHTDTSLCTILHQVGNNGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRF 120

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            S+ HRVMI+E ++   S+AYF+ PPG +Y++PYCKPLS+T Q P+Y+ VNVKEYF IKA
Sbjct: 121 PSILHRVMIQENKEHRFSLAYFYDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKA 180

Query: 181 KASGKGIAALTI 193
           K +GKG+ A+ I
Sbjct: 181 KKTGKGLPAIKI 192

[Close]

BLAST of CmoCh05G006810 vs. NCBI nr
Match: gi|659088742|ref|XP_008445138.1| (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 255.8 bits (652), Expect = 6.2e-65
Identity = 116/192 (60.42%), Postives = 151/192 (78.65%), Query Frame = 1


Query: 1   MEEYQLKMKALADKLISLIFKFLGISDEEMVKKLSYIDPATGKPHLALRLNSFPPCPEPS 60
           MEEYQ +MK L +++I LI KFLGIS+EEM+K L+  D + GKP++ALRLNS+PPCP+P 
Sbjct: 160 MEEYQRQMKGLGERIIRLILKFLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPG 219

Query: 61  KVIGLAAHTDTSLFTMLHQARREGLQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRF 120
           +V+GLAAHTDTSL T+LHQ R  GLQI  +  GW+PL+P S  L++NVGD L I+SNGRF
Sbjct: 220 QVMGLAAHTDTSLCTILHQVRSNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRF 279

Query: 121 HSVPHRVMIRETEKTTMSMAYFFHPPGHLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKA 180
            S+ HRVMI+E ++   S+AYF+ PPG +Y++PYCKPLS+T Q P+Y+ VNVKEYF IKA
Sbjct: 280 TSILHRVMIQENKEHRFSLAYFYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKA 339

Query: 181 KASGKGIAALTI 193
           K  G G+ A+ I
Sbjct: 340 KKMGSGLPAIKI 351

The following BLAST results are available for this feature:

BLAST of CmoCh05G006810 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G3OX1_ARATH	3.4e-31	36.84	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2	[more]
G3OX4_ARATH	2.2e-30	36.41	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1	[more]
G3OX_PEA	4.9e-30	36.84	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1	[more]
G3O23_WHEAT	6.4e-30	37.76	Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum GN=GA3ox2-3 PE=2 SV=1	[more]
G3O22_WHEAT	1.4e-29	37.24	Gibberellin 3-beta-dioxygenase 2-2 OS=Triticum aestivum GN=GA3ox2-2 PE=2 SV=1	[more]

BLAST of CmoCh05G006810 vs. TrEMBL


Match Name	E-value	Identity	Description
O24417_CUCMA	1.4e-103	96.88	Gibberellin 2beta,3beta-hydroxylase OS=Cucurbita maxima PE=2 SV=1	[more]
Q9LM06_CITLA	9.3e-68	62.50	Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus GN=WGA3H PE=2 SV=1	[more]
A0A0A0LSC5_CUCSA	5.1e-66	60.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G379320 PE=4 SV=1	[more]
S0BE47_CUCSA	5.1e-66	60.94	GA 3-oxidase OS=Cucumis sativus GN=GA3ox4 PE=2 SV=1	[more]
E1AXG4_9ROSI	8.7e-58	55.73	Gibberellin 3-oxidase 1 (Fragment) OS=Marah macrocarpus PE=2 SV=1	[more]

BLAST of CmoCh05G006810 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G15550.1	1.9e-32	36.84	gibberellin 3-oxidase 1	[more]
AT1G80330.1	1.2e-31	36.41	gibberellin 3-oxidase 4	[more]
AT1G80340.1	8.9e-30	36.81	gibberellin 3-oxidase 2	[more]
AT4G21690.1	9.3e-27	36.41	gibberellin 3-oxidase 3	[more]
AT1G78550.1	3.9e-25	34.92	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of CmoCh05G006810 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|2289032\|gb\|AAB64347.1\|	2.0e-103	96.88	gibberellin 2beta,3beta-hydroxylase [Cucurbita maxima]	[more]
gi\|8894936\|gb\|AAF80661.1\|AF074710_1	1.3e-67	62.50	putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris]	[more]
gi\|568384792\|ref\|NP_001275523.1\|	7.3e-66	60.94	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus]	[more]
gi\|700207782\|gb\|KGN62901.1\|	7.3e-66	60.94	hypothetical protein Csa_2G379320 [Cucumis sativus]	[more]
gi\|659088742\|ref\|XP_008445138.1\|	6.2e-65	60.42	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001261	ArgE/DapE_CS
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR027443	IPNS-like

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008237	metallopeptidase activity
GO:0016491	oxidoreductase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh05G006810.1	CmoCh05G006810.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001261	ArgE/DapE/ACY1/CPG2/YscS, conserved site	PROSITE	PS00758	ARGE_DAPE_CPG2_1	coord: 63..72 scor
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 46..145 score: 3.0
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 43..145 score: 10
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 1..184 score: 1.6
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..189 score: 9.0
None	No IPR available	PANTHER	PTHR10209:SF197	GIBBERELLIN 3-BETA-DIOXYGENASE 3	coord: 1..189 score: 9.0
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 1..183 score: 6.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh05G006810	Csa2G379320	Cucumber (Chinese Long) v2	cmocuB778

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh05G006810	Cucumber (Chinese Long) v3	cmocucB0919
CmoCh05G006810	Watermelon (97103) v2	cmowmbB800
CmoCh05G006810	Wax gourd	cmowgoB0947
CmoCh05G006810	Cucurbita moschata (Rifu)	cmocmoB151
CmoCh05G006810	Cucurbita moschata (Rifu)	cmocmoB159
CmoCh05G006810	Cucurbita moschata (Rifu)	cmocmoB373
CmoCh05G006810	Cucumber (Gy14) v1	cgycmoB0461
CmoCh05G006810	Cucurbita maxima (Rimu)	cmacmoB194
CmoCh05G006810	Cucurbita maxima (Rimu)	cmacmoB486
CmoCh05G006810	Cucurbita maxima (Rimu)	cmacmoB786
CmoCh05G006810	Wild cucumber (PI 183967)	cmocpiB783
CmoCh05G006810	Melon (DHL92) v3.5.1	cmomeB719
CmoCh05G006810	Watermelon (Charleston Gray)	cmowcgB713
CmoCh05G006810	Watermelon (97103) v1	cmowmB746
CmoCh05G006810	Cucurbita pepo (Zucchini)	cmocpeB720
CmoCh05G006810	Cucurbita pepo (Zucchini)	cmocpeB747
CmoCh05G006810	Cucurbita pepo (Zucchini)	cmocpeB754
CmoCh05G006810	Bottle gourd (USVL1VR-Ls)	cmolsiB728
CmoCh05G006810	Cucumber (Gy14) v2	cgybcmoB239
CmoCh05G006810	Melon (DHL92) v3.6.1	cmomedB816
CmoCh05G006810	Silver-seed gourd	carcmoB0087
CmoCh05G006810	Silver-seed gourd	carcmoB1318
CmoCh05G006810	Silver-seed gourd	carcmoB1320