CmoCh04G028650 (gene) Cucurbita moschata (Rifu)

NameCmoCh04G028650
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionMatrix metalloproteinase
LocationCmo_Chr04 : 20386094 .. 20387993 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACATTATTTAGTGGTGTGTCCTACGATGGAGTAGTCCAAGAAACATGTCTGTCACGCTCAACTCAAATGAGTCCTGTAAATTTAGGACACATCTTCCCGTACTTCATTAGTAAGGCTAAGGCCAGTGAGTAGTAACTCAACTTTTGCAAACCCTAGTGGTCTGTGGGCGGGGAGGAGGACACACCTCTGCCAAATAAACCAAAAAAAAAAAAAAAAAAAAACATTTTGATACTTTCAATGGTAATTATTTTGAGAGAGAAAAAAAATTACGATTGGATTAGGTTGGGTTCATTATTTCATCAGTATTGTTTGGATGGAAACAATTACAATCCGAATAATTTGGTTAGTTCTAAAAAAAATTCAACTCGACCGTGAGATCTCACATTAATTGGAGAGAAGAATAAGTGCCAGCAACGACGCTAACCTCGAAGTAGAGTGGATTGTAAGATCTCACATTGATTGAAGAGGGGAACAAAACATTTTTTTTATGAGGGTGTGAAAACTTCTACTAGTAGAGCGTTTCAAAAAATTTGAGAAGAAGTCCCGAAGGAAAAAATACAAAGAAGATAATATTAGGTAGGTATGAACTTATGGTTTGAAAAAAACAATATTTGCTAGCGGTGAGGGTGGACTTAGAGTCCAAAAAGAATAATATCTCGCGCGCTTGGGTCCAAAAAGAACCATATTTGCTTGAACTTGGACTTGAGTTTGGGCTTAGCCGGTTACAGTTTTTGTTCTCACCATACATAACGATATCATACTAAAATGAGTATAAATTATATCCATTATCTGTCTTAATTATATATCATGAAATCATCCAACATGACAATGCCTGGCAATTATTTAATTTTGATGTATGCATATAGCAACTCAACCTTAGTTTTGTTTTTCTCCTTCATGCAATTTTCTTTTAAAAGGGCCCAAAACTCCAACCCATATTCATCACACACCCATCTTAGTTTGTCTCTATCTTGTCGTGAACATGGATTTGAAATCATGTCTCCAACTGATGTTCATACTTGTCGCCGCCATAGCACCTCACGCCATCATGGCGCGCCATCTTCACACCCACAAGTTTTCTTCATCTATATTTCCCCAACATCTTGTAGGAAGTGGGAAGGGCCACAACATGGAAGGAATCCACAACGTCAGAGCTTTCCTCCAACGTTATGGTTACTTGAGCACAAATGTGGATACCAAAGGCAACGCCTTTGATGACAACTTGGAATTCGCCCTTAAATCATACCAAAAATTCTTCAACCTCAACGTGAGTGGCATTTTGGATGTGGAGACTTTGGAGCTAATGTCGCAGCCTCGATGTGGAGTTCCCGACGTGTTTAGCAACGAGACGGGTGGGGGCGGAGACGTGGACTGGGATCACTCCATAGGAATTGATTACACATTTTTCCGTGAGTCGAAACCGAAATGGCGGGCGTCCAAATACAATCTGAAATACGCATTTGTTGGGAATTTTCCAGAACAGTACAAGGCTACAGTGCGTGAAGCCACAAACACTTGGAGTTCAGTCACTGGATTTACATTCACAGAAGGTAGTGTGGACAACACAGATGTTAAAATAAGCTTCGAAGTAGGAGATCATGGAGATGGGTATCCTTTCGTGGAGGGAAGTGGAGTATTGGCGCATGCTTTTGCGCCTGAAGATGGGAGACTCCACTTCAATACGCGGCATGCTTGGGCGGATGGAGCTTATCCTTATAAATATGATGTGGGGACTGTGGCGTTGCATGAGCTTGGACATGTTCTTGGGCTGGGCCACAGCGCCTTTAGATACGCTGTGATGTGGCCACATACACCTCCTAATTTTGTGACGGGCCTAGACAACGACGATGTTAATGGAATCATGGCTTTGTATAATGGGTTTGCGGATGCCTAG

mRNA sequence

ATGGAGACATTATTTAGTGGTGTGTCCTACGATGGAGTAGTCCAAGAAACATGTCTGTCACGCTCAACTCAAATGAGTCCTGTAAATTTAGGACACATCTTCCCGTACTTCATTAGTAAGGCTAAGGCCAGGCCCAAAACTCCAACCCATATTCATCACACACCCATCTTAGTTTGTCTCTATCTTGTCGTGAACATGGATTTGAAATCATGTCTCCAACTGATGTTCATACTTGTCGCCGCCATAGCACCTCACGCCATCATGGCGCGCCATCTTCACACCCACAAGTTTTCTTCATCTATATTTCCCCAACATCTTGTAGGAAGTGGGAAGGGCCACAACATGGAAGGAATCCACAACGTCAGAGCTTTCCTCCAACGTTATGGTTACTTGAGCACAAATGTGGATACCAAAGGCAACGCCTTTGATGACAACTTGGAATTCGCCCTTAAATCATACCAAAAATTCTTCAACCTCAACGTGAGTGGCATTTTGGATGTGGAGACTTTGGAGCTAATGTCGCAGCCTCGATGTGGAGTTCCCGACGTGTTTAGCAACGAGACGGGTGGGGGCGGAGACGTGGACTGGGATCACTCCATAGGAATTGATTACACATTTTTCCGTGAGTCGAAACCGAAATGGCGGGCGTCCAAATACAATCTGAAATACGCATTTGTTGGGAATTTTCCAGAACAGTACAAGGCTACAGTGCGTGAAGCCACAAACACTTGGAGTTCAGTCACTGGATTTACATTCACAGAAGGTAGTGTGGACAACACAGATGTTAAAATAAGCTTCGAAGTAGGAGATCATGGAGATGGGTATCCTTTCGTGGAGGGAAGTGGAGTATTGGCGCATGCTTTTGCGCCTGAAGATGGGAGACTCCACTTCAATACGCGGCATGCTTGGGCGGATGGAGCTTATCCTTATAAATATGATGTGGGGACTGTGGCGTTGCATGAGCTTGGACATGTTCTTGGGCTGGGCCACAGCGCCTTTAGATACGCTGTGATGTGGCCACATACACCTCCTAATTTTGTGACGGGCCTAGACAACGACGATGTTAATGGAATCATGGCTTTGTATAATGGGTTTGCGGATGCCTAG

Coding sequence (CDS)

ATGGAGACATTATTTAGTGGTGTGTCCTACGATGGAGTAGTCCAAGAAACATGTCTGTCACGCTCAACTCAAATGAGTCCTGTAAATTTAGGACACATCTTCCCGTACTTCATTAGTAAGGCTAAGGCCAGGCCCAAAACTCCAACCCATATTCATCACACACCCATCTTAGTTTGTCTCTATCTTGTCGTGAACATGGATTTGAAATCATGTCTCCAACTGATGTTCATACTTGTCGCCGCCATAGCACCTCACGCCATCATGGCGCGCCATCTTCACACCCACAAGTTTTCTTCATCTATATTTCCCCAACATCTTGTAGGAAGTGGGAAGGGCCACAACATGGAAGGAATCCACAACGTCAGAGCTTTCCTCCAACGTTATGGTTACTTGAGCACAAATGTGGATACCAAAGGCAACGCCTTTGATGACAACTTGGAATTCGCCCTTAAATCATACCAAAAATTCTTCAACCTCAACGTGAGTGGCATTTTGGATGTGGAGACTTTGGAGCTAATGTCGCAGCCTCGATGTGGAGTTCCCGACGTGTTTAGCAACGAGACGGGTGGGGGCGGAGACGTGGACTGGGATCACTCCATAGGAATTGATTACACATTTTTCCGTGAGTCGAAACCGAAATGGCGGGCGTCCAAATACAATCTGAAATACGCATTTGTTGGGAATTTTCCAGAACAGTACAAGGCTACAGTGCGTGAAGCCACAAACACTTGGAGTTCAGTCACTGGATTTACATTCACAGAAGGTAGTGTGGACAACACAGATGTTAAAATAAGCTTCGAAGTAGGAGATCATGGAGATGGGTATCCTTTCGTGGAGGGAAGTGGAGTATTGGCGCATGCTTTTGCGCCTGAAGATGGGAGACTCCACTTCAATACGCGGCATGCTTGGGCGGATGGAGCTTATCCTTATAAATATGATGTGGGGACTGTGGCGTTGCATGAGCTTGGACATGTTCTTGGGCTGGGCCACAGCGCCTTTAGATACGCTGTGATGTGGCCACATACACCTCCTAATTTTGTGACGGGCCTAGACAACGACGATGTTAATGGAATCATGGCTTTGTATAATGGGTTTGCGGATGCCTAG
BLAST of CmoCh04G028650 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 1.0e-44
Identity = 102/263 (38.78%), Postives = 146/263 (55.51%), Query Frame = 1

Query: 112 GHNMEGIHNVRAFLQRYGYLSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDVETLE 171
           G N +G+ NV+ +    GY+  N     + FDD L  A+K+YQK +NLNV+G  D+ TL+
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIP-NAPHFDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLK 107

Query: 172 LMSQPRCGVPDVF--SNETGGGGDVDWDHSIGIDYTFFRESKPKWRASKYNLKYAF--VG 231
            +  PRCGVPD+   +N+T   G +        DYTFF++  P+W+A    L YAF    
Sbjct: 108 QIMTPRCGVPDIIINTNKTTSFGMIS-------DYTFFKD-MPRWQAGTTQLTYAFSPEP 167

Query: 232 NFPEQYKATVREATNTWSSVTGFTFTE-GSVDNTDVKISFEVGDHGDGYPFVEGSGVLAH 291
              + +K+ +  A + W+ V    F E  S +  ++KI F   +HGD YPF    G+L H
Sbjct: 168 RLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGH 227

Query: 292 AFAPEDGRLHFNTRHAWADGAYPYK------YDVGTVALHELGHVLGLGHSAFRYAVMWP 351
           AFAP DGR HF+    W       K      +D+ +VA+HE+GH+LGLGHS+   A+M+P
Sbjct: 228 AFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYP 287

Query: 352 HTPPNF-VTGLDNDDVNGIMALY 363
             PP      L  DD++GI  LY
Sbjct: 288 SIPPRTRKVNLAQDDIDGIRKLY 301

BLAST of CmoCh04G028650 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 8.5e-39
Identity = 101/280 (36.07%), Postives = 147/280 (52.50%), Query Frame = 1

Query: 105 HLVGSGKGHNMEGIHNVRAFLQRYGYL-STNVDTKGNAFDDNLEFALKSYQKFFNLNVSG 164
           +  G   G N++G++ ++ + QR+GY+  T      + FDD L+ A++ YQ  FNLNV+G
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTG 105

Query: 165 ILDVETLELMSQPRCGVPDVFSNET--GGGGDVDWD--------HSIGIDYTFFRESKPK 224
            LD  T++ +  PRCG PDV +  +   GG    ++        H++   YT F   +P+
Sbjct: 106 ELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVK-RYTLF-PGEPR 165

Query: 225 WRASKYNLKYAFVGNFP--EQYKATVREATNTWSSVTGFTFT-EGSVDNTDVKISFEVGD 284
           W  ++ +L YAF    P  E+ K+    A   WS VT   FT   S   +D+ I F  GD
Sbjct: 166 WPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGD 225

Query: 285 HGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAWADG-------AYPYKYDVGTVALHELG 344
           HGDG PF    G LAHAF+P  G+ H +    W          +     D+ +VA+HE+G
Sbjct: 226 HGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIG 285

Query: 345 HVLGLGHSAFRYAVMWPH-TPPNFVTGLDNDDVNGIMALY 363
           H+LGLGHS+   ++M+P  T       L NDDV GI  LY
Sbjct: 286 HLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of CmoCh04G028650 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.1e-38
Identity = 100/274 (36.50%), Postives = 145/274 (52.92%), Query Frame = 1

Query: 106 LVGSGKGHNMEGIHNVRAFLQRYGYLSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGIL 165
           L G   G N+ G+  ++ + +R+GY++T  +   + FDD L+ A+ +YQK FNL V+G L
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDD-FDDVLQSAINTYQKNFNLKVTGKL 107

Query: 166 DVETLELMSQPRCGVPDVFS--NETGGGGDVDWDHSIGIDYTFFRESKPKWRASKYNLKY 225
           D  TL  + +PRCG PD+    +E  GG  +         Y+FF   KP+W   K +L Y
Sbjct: 108 DSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEK----YSFF-PGKPRWPKRKRDLTY 167

Query: 226 AFV--GNFPEQYKATVREATNTWSSVTGFTFTEG-SVDNTDVKISFEVGDHGDGYPFVEG 285
           AF    N  ++ K     A   W+ VT   FT   S+   D+ I F  G+HGDG PF   
Sbjct: 168 AFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGA 227

Query: 286 SGVLAHAFAPEDGRLHFNTRHAW--ADGAYPYK-------YDVGTVALHELGHVLGLGHS 345
            G LAHA +P  G LH +    W  ++G    +        D+ +VA+HE+GH+LGLGHS
Sbjct: 228 MGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHS 287

Query: 346 AFRYAVMWPH-TPPNFVTGLDNDDVNGIMALYNG 365
           +   A+M+P  +  +    L  DD+ GI  LY G
Sbjct: 288 SVEDAIMFPAISGGDRKVELAKDDIEGIQHLYGG 315

BLAST of CmoCh04G028650 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.5e-38
Identity = 111/315 (35.24%), Postives = 153/315 (48.57%), Query Frame = 1

Query: 74  LMFILVAAIAPHAIMARHLHTHKFSSSIFPQ-------------HLVGSGKGHNMEGIHN 133
           L F+L+    P+   AR+    + S++   Q              LV    G ++ G+  
Sbjct: 13  LCFVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSE 72

Query: 134 VRAFLQRYGYLSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGV 193
           ++ +L R+GY++   +   + FD  LE A+  YQ+   L ++G LD  T+ LMS PRCGV
Sbjct: 73  LKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGV 132

Query: 194 PDVFSNETGGGGDVDWDHSIGIDYTFFRESKPKWRASKYNLKYAFVGNF------PEQYK 253
            D            D+ H+    YT+F   KPKW  ++  L YA            E  K
Sbjct: 133 SDTHMTINN-----DFLHTTA-HYTYF-NGKPKW--NRDTLTYAISKTHKLDYLTSEDVK 192

Query: 254 ATVREATNTWSSVTGFTFTE-GSVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDG 313
              R A + WSSV   +F E       D+KI F  GDHGDG PF    G LAHAFAPE+G
Sbjct: 193 TVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENG 252

Query: 314 RLHFNTRHAW-----ADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNF-V 363
           RLH +    W       G+     D+ +VA HE+GH+LGLGHS+   AVM+P   P    
Sbjct: 253 RLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKK 312

BLAST of CmoCh04G028650 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 6.3e-34
Identity = 110/339 (32.45%), Postives = 166/339 (48.97%), Query Frame = 1

Query: 68  LKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHL--------------VGSGKGH 127
           ++ C+ ++F+L  A +P   ++   +T+  SS+I PQ L               G   G 
Sbjct: 2   VRICVFMVFLLFFAPSP---VSAGFYTN--SSAIPPQLLRNATGNPWNSFLNFTGCHAGK 61

Query: 128 NMEGIHNVRAFLQRYGYLSTNVDTKGNA---FDDNLEFALKSYQKFFNLNVSGILDVETL 187
             +G++ ++ + Q +GY+ T  +  GN    FDD L+ A++ YQ+ F LNV+G+LD  TL
Sbjct: 62  KYDGLYMLKQYFQHFGYI-TETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTL 121

Query: 188 ELMSQPRCGVPDVFSN-----------ETGGGGDVDWDHSIGIDYTFFRESKPKWRASKY 247
           + +  PRCG PDV +            E    G     H++   Y+FF   +P+W  ++ 
Sbjct: 122 KHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVK-HYSFF-PGEPRWPRNRR 181

Query: 248 NLKYAF--VGNFPEQYKATVREATNTWSSVTGFTFTE-GSVDNTDVKISFEVGDHGDGYP 307
           +L YAF       E+ K+    A   W  VT  TFT       +D+ I F  G+HGDG P
Sbjct: 182 DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEP 241

Query: 308 FVEGSGVLAHAFAPEDGRLHFNTRHAW------ADG--AYPYKYDVGTVALHELGHVLGL 363
           F      LAHAF+P  G  H +    W       DG  +     D+ +VA+HE+GH+LGL
Sbjct: 242 FDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGL 301

BLAST of CmoCh04G028650 vs. TrEMBL
Match: A0A0A0KS15_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632090 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.1e-69
Identity = 154/314 (49.04%), Postives = 189/314 (60.19%), Query Frame = 1

Query: 66  MDLKSCLQL---MFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVR 125
           MD KS   L   + +L+A+IA   I AR LH  K S  +FPQHL+GS KGHN+EGIH ++
Sbjct: 1   MDFKSSSDLQIFLLLLLASIAFPFIWARDLHDIKKSQLLFPQHLLGSSKGHNIEGIHTIK 60

Query: 126 AFLQRYGYLSTN---VDTKG---NAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQP 185
             LQRYGYLS N   +DT G   NAFDD+LE A+K YQ FF L  SG+LD+ETL  MSQ 
Sbjct: 61  MHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQA 120

Query: 186 RCGVPDVFSNETGGGGDVDWDHSIGIDYTFFRESKPKWRAS-KYNLKYAFVGNFPEQYKA 245
           RC VPD+F N          +  IG  YTFF   + KW  S  Y L YA V NFPE++K 
Sbjct: 121 RCSVPDIFENNENETSVTTSNLHIGSKYTFF-PGRVKWPDSLNYRLTYALVNNFPEEFKE 180

Query: 246 TVREATNTWSSVTGFTFTEGSV-DNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGR 305
           +VR A   W   + F FTE S  +  +++ISFE G HGD +PF + S  LAH FAP DGR
Sbjct: 181 SVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGR 240

Query: 306 LHFNTRHAWADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDD 365
            HFN    ++       Y + TVALHELGH  GL HS    A+M+P  P N    LD DD
Sbjct: 241 FHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDD 300

Query: 366 VNGIMALYNGFADA 369
           + G+  LY+GF  A
Sbjct: 301 IEGLWELYDGFGVA 313

BLAST of CmoCh04G028650 vs. TrEMBL
Match: A0A0A0KX75_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632100 PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.5e-69
Identity = 151/311 (48.55%), Postives = 197/311 (63.34%), Query Frame = 1

Query: 66  MDLKSC-LQLMFILVAAIAP-HAIMARHL-HTHKFSSSIFPQHLVGSGKGHNMEGIHNVR 125
           MD KS  LQL  +L+  +   H I +R L H H  S  +FP++L+GS KGHN+EG H ++
Sbjct: 1   MDFKSLHLQLFLLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60

Query: 126 AFLQRYGYLSTN---VDTKG---NAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQP 185
            +LQRYGYLS +   +DT G   NA+D+ LE ++K YQKFF LN SGILD ETL  MSQ 
Sbjct: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120

Query: 186 RCGVPDVFSNETGGGGDVDWDHSIGIDYTFFRESKPKWRASK-YNLKYAFVGNFPEQYKA 245
           RC VPD F ++         +  +G  + FF   +PKW  SK Y+L ++F+ NFP  +K 
Sbjct: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFF-PGRPKWPDSKNYSLTFSFINNFPGIFKG 180

Query: 246 TVREATNTWSSVTGFTFTE-GSVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGR 305
            V +A   W   + F FTE    + +D+KISFEVGDHGDG+PF    GVLAHAF P DGR
Sbjct: 181 EVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPF--RVGVLAHAFTPTDGR 240

Query: 306 LHFNTRHAWADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDD 365
           LHFN    ++      KY V +VALHELGH LGL H+    A+M+P  PPNF   +++DD
Sbjct: 241 LHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDD 300

BLAST of CmoCh04G028650 vs. TrEMBL
Match: A0A0A0KU02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 2.3e-67
Identity = 148/302 (49.01%), Postives = 190/302 (62.91%), Query Frame = 1

Query: 66  MDLKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVRAFL 125
           M L+  LQL F+L+A+IAPH I AR  HTH  S      HL GS  G+N+EGIHNV+ +L
Sbjct: 1   MALQFSLQL-FLLIASIAPHVITARVRHTHISS------HLQGSRIGNNIEGIHNVKLYL 60

Query: 126 QRYGYLSTNVDT--KGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGVPDV 185
           +RYGYL TNV++    N FD  LE A+K++QK+ +LNVSG++D ETL LMS PRCG+PD+
Sbjct: 61  KRYGYL-TNVESTNSNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDI 120

Query: 186 FSNETGGGGDVDWDHS-IGIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATN 245
             N      +V  + S     +TFF ++  KW  SKYNL+Y F+ +FP  +K  V  A  
Sbjct: 121 MHNINVMDNNVQMNSSSFHSHFTFFPDNL-KWPVSKYNLRYTFLDDFPNDFKEPVMNAME 180

Query: 246 TWSSVTGFTFTEG-SVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRH 305
            W+  + F F+E       D+  +F  G+HGDGYPF    GVLAHAF P DGR+HF+   
Sbjct: 181 QWALFSLFRFSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDE 240

Query: 306 AWADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVT-GLDNDDVNGIMA 363
            WADG+     +VG VALHELGHVLGL HS  R A+MWP+      T GL  DD+ GI  
Sbjct: 241 DWADGSVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQT 293

BLAST of CmoCh04G028650 vs. TrEMBL
Match: A0A0A0KS35_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.4e-64
Identity = 142/304 (46.71%), Postives = 186/304 (61.18%), Query Frame = 1

Query: 66  MDLKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNM-EGIHNVRAF 125
           M  K CLQL F+L+A + PH   AR LH HK S  +FP+HL+GS K  ++ EGIH V+A+
Sbjct: 1   MAAKLCLQL-FLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAY 60

Query: 126 LQRYGYLSTNVDTK--GNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGVPD 185
           LQRYGY S   +     +AFDD+LE A+KSYQKF NL VSG+LD ETL+ MS+PRCGV D
Sbjct: 61  LQRYGYFSNENENNLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVAD 120

Query: 186 VFSNETGGGGD-VDWDHSIGIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREAT 245
            F +     G+  +    IG  +  F   K KW   K++L Y FV N+P ++ A V  A 
Sbjct: 121 NFQSVAQQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAF 180

Query: 246 NTWSSVTGFTFT-EGSVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTR 305
           + W++ + FTF+    +   D+ +SFE GDHGDG PF    G+L HAF P DGR+HF+  
Sbjct: 181 DKWAANSKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDAD 240

Query: 306 HAWAD--GAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNF-VTGLDNDDVNG 362
             WA+        +D  +VALHE+GH LGLGHS F  AVMW +       T L  DD+ G
Sbjct: 241 EQWAEIGSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEG 300

BLAST of CmoCh04G028650 vs. TrEMBL
Match: A0A0A0KVC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632060 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.9e-63
Identity = 144/305 (47.21%), Postives = 189/305 (61.97%), Query Frame = 1

Query: 66  MDLKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNM-EGIHNVRAF 125
           M  K CLQL  +L+A I PH   ARHLH  K S  +FP+HL+GS K  ++ EGIH V+A+
Sbjct: 1   MASKLCLQLC-LLLAFITPHLTSARHLHNRKPSPFLFPKHLIGSSKNDSIVEGIHKVKAY 60

Query: 126 LQRYGYLSTNVDTK--GNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGVPD 185
           LQRYGYLS   + K   +AFDD+LE A+KSYQKF NL VSG+LD ETL+ MS+PRCGV D
Sbjct: 61  LQRYGYLSNENEKKLSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVRD 120

Query: 186 VFSNETGGGGDVDWDHSI---GIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVRE 245
            + +  G     + + +I   G  YTF+  +   W A + +L Y F+ +FP Q+  TVR 
Sbjct: 121 TYVS-VGQQEHENKNTNIEIGGSHYTFY-YNHVTWPAERRHLSYGFIHDFPPQHVDTVRR 180

Query: 246 ATNTWSSVTGFT-FTEGSVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFN 305
           A  TW   T F+ F    V   D+ +SFE G+HGD  PF    G+LAH+    DGR+HF+
Sbjct: 181 AFQTWEDNTKFSFFLSPRVQTADILVSFERGEHGDHEPFDGEGGILAHSLGAVDGRVHFD 240

Query: 306 TRHAWADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNF-VTGLDNDDVNG 363
               W +G    +YD+ T+ALHE+GH LGLGHS+   A+MW +    F  T L  DD+ G
Sbjct: 241 ADDRW-EGDPMERYDLETLALHEIGHALGLGHSSSSLAIMWAYMYKGFPKTRLTIDDIEG 300

BLAST of CmoCh04G028650 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 162.5 bits (410), Expect = 4.8e-40
Identity = 101/280 (36.07%), Postives = 147/280 (52.50%), Query Frame = 1

Query: 105 HLVGSGKGHNMEGIHNVRAFLQRYGYL-STNVDTKGNAFDDNLEFALKSYQKFFNLNVSG 164
           +  G   G N++G++ ++ + QR+GY+  T      + FDD L+ A++ YQ  FNLNV+G
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTG 105

Query: 165 ILDVETLELMSQPRCGVPDVFSNET--GGGGDVDWD--------HSIGIDYTFFRESKPK 224
            LD  T++ +  PRCG PDV +  +   GG    ++        H++   YT F   +P+
Sbjct: 106 ELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVK-RYTLF-PGEPR 165

Query: 225 WRASKYNLKYAFVGNFP--EQYKATVREATNTWSSVTGFTFT-EGSVDNTDVKISFEVGD 284
           W  ++ +L YAF    P  E+ K+    A   WS VT   FT   S   +D+ I F  GD
Sbjct: 166 WPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGD 225

Query: 285 HGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAWADG-------AYPYKYDVGTVALHELG 344
           HGDG PF    G LAHAF+P  G+ H +    W          +     D+ +VA+HE+G
Sbjct: 226 HGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIG 285

Query: 345 HVLGLGHSAFRYAVMWPH-TPPNFVTGLDNDDVNGIMALY 363
           H+LGLGHS+   ++M+P  T       L NDDV GI  LY
Sbjct: 286 HLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of CmoCh04G028650 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 162.2 bits (409), Expect = 6.3e-40
Identity = 100/274 (36.50%), Postives = 145/274 (52.92%), Query Frame = 1

Query: 106 LVGSGKGHNMEGIHNVRAFLQRYGYLSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGIL 165
           L G   G N+ G+  ++ + +R+GY++T  +   + FDD L+ A+ +YQK FNL V+G L
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDD-FDDVLQSAINTYQKNFNLKVTGKL 107

Query: 166 DVETLELMSQPRCGVPDVFS--NETGGGGDVDWDHSIGIDYTFFRESKPKWRASKYNLKY 225
           D  TL  + +PRCG PD+    +E  GG  +         Y+FF   KP+W   K +L Y
Sbjct: 108 DSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEK----YSFF-PGKPRWPKRKRDLTY 167

Query: 226 AFV--GNFPEQYKATVREATNTWSSVTGFTFTEG-SVDNTDVKISFEVGDHGDGYPFVEG 285
           AF    N  ++ K     A   W+ VT   FT   S+   D+ I F  G+HGDG PF   
Sbjct: 168 AFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGA 227

Query: 286 SGVLAHAFAPEDGRLHFNTRHAW--ADGAYPYK-------YDVGTVALHELGHVLGLGHS 345
            G LAHA +P  G LH +    W  ++G    +        D+ +VA+HE+GH+LGLGHS
Sbjct: 228 MGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHS 287

Query: 346 AFRYAVMWPH-TPPNFVTGLDNDDVNGIMALYNG 365
           +   A+M+P  +  +    L  DD+ GI  LY G
Sbjct: 288 SVEDAIMFPAISGGDRKVELAKDDIEGIQHLYGG 315

BLAST of CmoCh04G028650 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 161.8 bits (408), Expect = 8.2e-40
Identity = 111/315 (35.24%), Postives = 153/315 (48.57%), Query Frame = 1

Query: 74  LMFILVAAIAPHAIMARHLHTHKFSSSIFPQ-------------HLVGSGKGHNMEGIHN 133
           L F+L+    P+   AR+    + S++   Q              LV    G ++ G+  
Sbjct: 13  LCFVLILFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSE 72

Query: 134 VRAFLQRYGYLSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGV 193
           ++ +L R+GY++   +   + FD  LE A+  YQ+   L ++G LD  T+ LMS PRCGV
Sbjct: 73  LKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGV 132

Query: 194 PDVFSNETGGGGDVDWDHSIGIDYTFFRESKPKWRASKYNLKYAFVGNF------PEQYK 253
            D            D+ H+    YT+F   KPKW  ++  L YA            E  K
Sbjct: 133 SDTHMTINN-----DFLHTTA-HYTYF-NGKPKW--NRDTLTYAISKTHKLDYLTSEDVK 192

Query: 254 ATVREATNTWSSVTGFTFTE-GSVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDG 313
              R A + WSSV   +F E       D+KI F  GDHGDG PF    G LAHAFAPE+G
Sbjct: 193 TVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENG 252

Query: 314 RLHFNTRHAW-----ADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNF-V 363
           RLH +    W       G+     D+ +VA HE+GH+LGLGHS+   AVM+P   P    
Sbjct: 253 RLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKK 312

BLAST of CmoCh04G028650 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 146.4 bits (368), Expect = 3.6e-35
Identity = 110/339 (32.45%), Postives = 166/339 (48.97%), Query Frame = 1

Query: 68  LKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHL--------------VGSGKGH 127
           ++ C+ ++F+L  A +P   ++   +T+  SS+I PQ L               G   G 
Sbjct: 2   VRICVFMVFLLFFAPSP---VSAGFYTN--SSAIPPQLLRNATGNPWNSFLNFTGCHAGK 61

Query: 128 NMEGIHNVRAFLQRYGYLSTNVDTKGNA---FDDNLEFALKSYQKFFNLNVSGILDVETL 187
             +G++ ++ + Q +GY+ T  +  GN    FDD L+ A++ YQ+ F LNV+G+LD  TL
Sbjct: 62  KYDGLYMLKQYFQHFGYI-TETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTL 121

Query: 188 ELMSQPRCGVPDVFSN-----------ETGGGGDVDWDHSIGIDYTFFRESKPKWRASKY 247
           + +  PRCG PDV +            E    G     H++   Y+FF   +P+W  ++ 
Sbjct: 122 KHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVK-HYSFF-PGEPRWPRNRR 181

Query: 248 NLKYAF--VGNFPEQYKATVREATNTWSSVTGFTFTE-GSVDNTDVKISFEVGDHGDGYP 307
           +L YAF       E+ K+    A   W  VT  TFT       +D+ I F  G+HGDG P
Sbjct: 182 DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEP 241

Query: 308 FVEGSGVLAHAFAPEDGRLHFNTRHAW------ADG--AYPYKYDVGTVALHELGHVLGL 363
           F      LAHAF+P  G  H +    W       DG  +     D+ +VA+HE+GH+LGL
Sbjct: 242 FDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGL 301

BLAST of CmoCh04G028650 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 145.2 bits (365), Expect = 7.9e-35
Identity = 96/258 (37.21%), Postives = 129/258 (50.00%), Query Frame = 1

Query: 118 IHNVRAFLQRYGYLSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPR 177
           I  ++  LQ+YGYL  N ++     D + E AL  YQK   L ++G  D +TL  +  PR
Sbjct: 50  IPEIKRHLQQYGYLPQNKESD----DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPR 109

Query: 178 CGVPDVFSNETGGGGDVDWDHSIGIDYTFFRESKPKW-RASKYNLKYAFVGN------FP 237
           CG PD    +T            G  Y +F   +P+W R     L YAF          P
Sbjct: 110 CGFPDDVEPKTA-------PFHTGKKYVYF-PGRPRWTRDVPLKLTYAFSQENLTPYLAP 169

Query: 238 EQYKATVREATNTWSSVTGFTFTEGS-VDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFA 297
              +   R A   W+SV   +F E       D+KI F  GDHGDG PF    GVLAH F+
Sbjct: 170 TDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFS 229

Query: 298 PEDGRLHFNTRHAWA----DGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPP- 357
           PE+GRLH +    WA    +       D+ +VA+HE+GHVLGLGHS+ + A M+P   P 
Sbjct: 230 PENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPR 289

Query: 358 NFVTGLDNDDVNGIMALY 363
           +    L+ DDV G+ +LY
Sbjct: 290 SKKVNLNMDDVVGVQSLY 295

BLAST of CmoCh04G028650 vs. NCBI nr
Match: gi|449449423|ref|XP_004142464.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 271.9 bits (694), Expect = 1.6e-69
Identity = 154/314 (49.04%), Postives = 189/314 (60.19%), Query Frame = 1

Query: 66  MDLKSCLQL---MFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVR 125
           MD KS   L   + +L+A+IA   I AR LH  K S  +FPQHL+GS KGHN+EGIH ++
Sbjct: 1   MDFKSSSDLQIFLLLLLASIAFPFIWARDLHDIKKSQLLFPQHLLGSSKGHNIEGIHTIK 60

Query: 126 AFLQRYGYLSTN---VDTKG---NAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQP 185
             LQRYGYLS N   +DT G   NAFDD+LE A+K YQ FF L  SG+LD+ETL  MSQ 
Sbjct: 61  MHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQA 120

Query: 186 RCGVPDVFSNETGGGGDVDWDHSIGIDYTFFRESKPKWRAS-KYNLKYAFVGNFPEQYKA 245
           RC VPD+F N          +  IG  YTFF   + KW  S  Y L YA V NFPE++K 
Sbjct: 121 RCSVPDIFENNENETSVTTSNLHIGSKYTFF-PGRVKWPDSLNYRLTYALVNNFPEEFKE 180

Query: 246 TVREATNTWSSVTGFTFTEGSV-DNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGR 305
           +VR A   W   + F FTE S  +  +++ISFE G HGD +PF + S  LAH FAP DGR
Sbjct: 181 SVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGR 240

Query: 306 LHFNTRHAWADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDD 365
            HFN    ++       Y + TVALHELGH  GL HS    A+M+P  P N    LD DD
Sbjct: 241 FHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDD 300

Query: 366 VNGIMALYNGFADA 369
           + G+  LY+GF  A
Sbjct: 301 IEGLWELYDGFGVA 313

BLAST of CmoCh04G028650 vs. NCBI nr
Match: gi|700197234|gb|KGN52411.1| (hypothetical protein Csa_5G632100 [Cucumis sativus])

HSP 1 Score: 270.8 bits (691), Expect = 3.6e-69
Identity = 151/311 (48.55%), Postives = 197/311 (63.34%), Query Frame = 1

Query: 66  MDLKSC-LQLMFILVAAIAP-HAIMARHL-HTHKFSSSIFPQHLVGSGKGHNMEGIHNVR 125
           MD KS  LQL  +L+  +   H I +R L H H  S  +FP++L+GS KGHN+EG H ++
Sbjct: 1   MDFKSLHLQLFLLLLTFLLLFHPISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIK 60

Query: 126 AFLQRYGYLSTN---VDTKG---NAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQP 185
            +LQRYGYLS +   +DT G   NA+D+ LE ++K YQKFF LN SGILD ETL  MSQ 
Sbjct: 61  TYLQRYGYLSKSYNIIDTNGVYNNAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQS 120

Query: 186 RCGVPDVFSNETGGGGDVDWDHSIGIDYTFFRESKPKWRASK-YNLKYAFVGNFPEQYKA 245
           RC VPD F ++         +  +G  + FF   +PKW  SK Y+L ++F+ NFP  +K 
Sbjct: 121 RCSVPDFFESDDNETSMTTSNLHMGSRFKFF-PGRPKWPDSKNYSLTFSFINNFPGIFKG 180

Query: 246 TVREATNTWSSVTGFTFTE-GSVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGR 305
            V +A   W   + F FTE    + +D+KISFEVGDHGDG+PF    GVLAHAF P DGR
Sbjct: 181 EVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPF--RVGVLAHAFTPTDGR 240

Query: 306 LHFNTRHAWADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDD 365
           LHFN    ++      KY V +VALHELGH LGL H+    A+M+P  PPNF   +++DD
Sbjct: 241 LHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDD 300

BLAST of CmoCh04G028650 vs. NCBI nr
Match: gi|449449425|ref|XP_004142465.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 264.2 bits (674), Expect = 3.3e-67
Identity = 148/302 (49.01%), Postives = 190/302 (62.91%), Query Frame = 1

Query: 66  MDLKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVRAFL 125
           M L+  LQL F+L+A+IAPH I AR  HTH  S      HL GS  G+N+EGIHNV+ +L
Sbjct: 1   MALQFSLQL-FLLIASIAPHVITARVRHTHISS------HLQGSRIGNNIEGIHNVKLYL 60

Query: 126 QRYGYLSTNVDT--KGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGVPDV 185
           +RYGYL TNV++    N FD  LE A+K++QK+ +LNVSG++D ETL LMS PRCG+PD+
Sbjct: 61  KRYGYL-TNVESTNSNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDI 120

Query: 186 FSNETGGGGDVDWDHS-IGIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATN 245
             N      +V  + S     +TFF ++  KW  SKYNL+Y F+ +FP  +K  V  A  
Sbjct: 121 MHNINVMDNNVQMNSSSFHSHFTFFPDNL-KWPVSKYNLRYTFLDDFPNDFKEPVMNAME 180

Query: 246 TWSSVTGFTFTEG-SVDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRH 305
            W+  + F F+E       D+  +F  G+HGDGYPF    GVLAHAF P DGR+HF+   
Sbjct: 181 QWALFSLFRFSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDE 240

Query: 306 AWADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVT-GLDNDDVNGIMA 363
            WADG+     +VG VALHELGHVLGL HS  R A+MWP+      T GL  DD+ GI  
Sbjct: 241 DWADGSVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQT 293

BLAST of CmoCh04G028650 vs. NCBI nr
Match: gi|659118025|ref|XP_008458911.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 263.8 bits (673), Expect = 4.3e-67
Identity = 152/314 (48.41%), Postives = 188/314 (59.87%), Query Frame = 1

Query: 66  MDLKSCLQLMFILVAAIAPHAIMARHLHTHKFSSSIFPQHLVGSGKGHNMEGIHNVRAFL 125
           M  K CLQL F+L A I PH   ARHL  HK S  +FP+HL+GS K  N EGIH V+A+L
Sbjct: 1   MGSKLCLQL-FLLFAFITPHLTSARHLRNHKPSRLLFPEHLIGSRKNDNFEGIHKVKAYL 60

Query: 126 QRYGYLSTNVDTKGN-----AFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGV 185
           QRYGYLS   D K N     AFDD+LE A+KSYQK F LNVSG+LD ETL+ M++PRCGV
Sbjct: 61  QRYGYLS---DEKENNLSIDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGV 120

Query: 186 PDVF---------SNETGGGGDVDWDHSIGIDYTFFRESKPKWRASKYNLKYAFVGNFPE 245
            D F         +N T        +  IG  +  F  +K KW   K +L Y F+ N+P 
Sbjct: 121 TDDFILVAQQDHENNNT--------NIQIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPP 180

Query: 246 QYKATVREATNTWSSVTGFTFTEGS-VDNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAP 305
           ++ A V  A +TW + T FTF+  + V   D+ +SFE GDHGDG PF    G L HAF  
Sbjct: 181 EHAAAVIRAFDTWEANTKFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGA 240

Query: 306 EDGRLHFNTRHAWADGAYPYKY-DVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFV-T 363
            DGR+HF+    W  G++  +Y DV +VALHE+GH LGLGHS    AVMW +       T
Sbjct: 241 IDGRVHFDGDEQWETGSFWQEYFDVESVALHEIGHALGLGHSREPSAVMWANMDSGVTKT 300

BLAST of CmoCh04G028650 vs. NCBI nr
Match: gi|659118021|ref|XP_008458908.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 263.5 bits (672), Expect = 5.7e-67
Identity = 149/312 (47.76%), Postives = 192/312 (61.54%), Query Frame = 1

Query: 66  MDLKSC-LQLMFILVAAIAPHAIMARHL-HTHKFSSSIFPQHLVGSGKGHNMEGIHNVRA 125
           MD KS  LQL  +L +    H I +R L H H  S  +FP++L+GS KGHN+EG H ++ 
Sbjct: 1   MDFKSFYLQLFLLLTSLSLFHPISSRDLNHIHGPSQFLFPKYLLGSRKGHNIEGTHAIKT 60

Query: 126 FLQRYGYLSTN---VDTKG---NAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPR 185
           +LQ YGYLS N   +DT G   NA+D+ LE ++K YQKFF LN SGILD ETL  MSQPR
Sbjct: 61  YLQHYGYLSKNYNIIDTNGVYNNAYDEQLECSVKKYQKFFKLNESGILDRETLSQMSQPR 120

Query: 186 CGVPDVFSNETGGGGDVDWDHSIGIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATV 245
           C VPD+F +E         +  I   Y FF   +PKW  S+ NL Y+ + NFPE+++A +
Sbjct: 121 CSVPDIFESEDNETSITTSNLHIRSRYAFF-PGRPKWSDSR-NLTYSLIKNFPEKFEAAI 180

Query: 246 REATNTWSSVTGFTFTEGSV-DNTDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLH 305
            +A   W   + F FT  S    +D+ ISFEVGDHGDG+PF    GVLAHAF+PEDGRLH
Sbjct: 181 VDAFMLWFLRSRFCFTYVSEGQKSDITISFEVGDHGDGHPFK--GGVLAHAFSPEDGRLH 240

Query: 306 FNTRHAWADGAYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDDVN 365
           FN   +++      KY + TVA HELGH LGL H+    A+M+P  P NF   L  DDV+
Sbjct: 241 FNGDKSFSSDLEEGKYHIRTVAAHELGHSLGLAHTNIEAAIMYPTLPSNFSKILSQDDVD 300

Query: 366 GIMALYNGFADA 369
           G+   Y    D+
Sbjct: 301 GLWNFYGQSEDS 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MEP1_SOYBN1.0e-4438.78Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
2MMP_ARATH8.5e-3936.07Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
5MMP_ARATH1.1e-3836.50Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
1MMP_ARATH1.5e-3835.24Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
3MMP_ARATH6.3e-3432.45Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KS15_CUCSA1.1e-6949.04Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632090 PE=3 SV=1[more]
A0A0A0KX75_CUCSA2.5e-6948.55Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632100 PE=3 SV=1[more]
A0A0A0KU02_CUCSA2.3e-6749.01Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1[more]
A0A0A0KS35_CUCSA2.4e-6446.71Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1[more]
A0A0A0KVC8_CUCSA5.9e-6347.21Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632060 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G70170.14.8e-4036.07 matrix metalloproteinase[more]
AT1G59970.16.3e-4036.50 Matrixin family protein[more]
AT4G16640.18.2e-4035.24 Matrixin family protein[more]
AT1G24140.13.6e-3532.45 Matrixin family protein[more]
AT2G45040.17.9e-3537.21 Matrixin family protein[more]
Match NameE-valueIdentityDescription
gi|449449423|ref|XP_004142464.1|1.6e-6949.04PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|700197234|gb|KGN52411.1|3.6e-6948.55hypothetical protein Csa_5G632100 [Cucumis sativus][more]
gi|449449425|ref|XP_004142465.1|3.3e-6749.01PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|659118025|ref|XP_008458911.1|4.3e-6748.41PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|659118021|ref|XP_008458908.1|5.7e-6747.76PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001818Pept_M10_metallopeptidase
IPR002477Peptidoglycan-bd-like
IPR006026Peptidase_Metallo
IPR021158Pept_M10A_Zn_BS
IPR021190Pept_M10A
IPR024079MetalloPept_cat_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G028650.1CmoCh04G028650.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 214..362
score: 1.7
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 118..175
score: 7.3
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 115..173
score: 2.3
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 111..183
score: 5.89
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 210..364
score: 1.4
IPR021158Peptidase M10A, cysteine switch, zinc binding sitePROSITEPS00546CYSTEINE_SWITCHcoord: 176..183
scor
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 350..363
score: 7.3E-33coord: 173..186
score: 7.3E-33coord: 317..342
score: 7.3E-33coord: 260..288
score: 7.3E-33coord: 237..252
score: 7.3
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 176..363
score: 5.3
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 103..362
score: 8.8E-86coord: 66..87
score: 8.8
NoneNo IPR availablePANTHERPTHR10201:SF152SUBFAMILY NOT NAMEDcoord: 103..362
score: 8.8E-86coord: 66..87
score: 8.8
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 211..363
score: 4.16

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh04G028650Cucumber (Chinese Long) v2cmocuB680
CmoCh04G028650Cucumber (Chinese Long) v2cmocuB695
CmoCh04G028650Cucumber (Chinese Long) v2cmocuB714
CmoCh04G028650Melon (DHL92) v3.5.1cmomeB624
CmoCh04G028650Melon (DHL92) v3.5.1cmomeB660
CmoCh04G028650Melon (DHL92) v3.5.1cmomeB677
CmoCh04G028650Watermelon (Charleston Gray)cmowcgB608
CmoCh04G028650Watermelon (97103) v1cmowmB646
CmoCh04G028650Cucurbita pepo (Zucchini)cmocpeB657
CmoCh04G028650Cucurbita pepo (Zucchini)cmocpeB673
CmoCh04G028650Cucurbita pepo (Zucchini)cmocpeB696
CmoCh04G028650Cucurbita pepo (Zucchini)cmocpeB704
CmoCh04G028650Bottle gourd (USVL1VR-Ls)cmolsiB640
CmoCh04G028650Bottle gourd (USVL1VR-Ls)cmolsiB641
CmoCh04G028650Bottle gourd (USVL1VR-Ls)cmolsiB647
CmoCh04G028650Cucumber (Gy14) v2cgybcmoB122
CmoCh04G028650Cucumber (Gy14) v2cgybcmoB395
CmoCh04G028650Cucumber (Gy14) v2cgybcmoB625
CmoCh04G028650Melon (DHL92) v3.6.1cmomedB713
CmoCh04G028650Melon (DHL92) v3.6.1cmomedB747
CmoCh04G028650Melon (DHL92) v3.6.1cmomedB766
CmoCh04G028650Silver-seed gourdcarcmoB0370
CmoCh04G028650Silver-seed gourdcarcmoB0501
CmoCh04G028650Silver-seed gourdcarcmoB0940
CmoCh04G028650Silver-seed gourdcarcmoB1354
CmoCh04G028650Cucumber (Chinese Long) v3cmocucB0802
CmoCh04G028650Cucumber (Chinese Long) v3cmocucB0824
CmoCh04G028650Cucumber (Chinese Long) v3cmocucB0846
CmoCh04G028650Watermelon (97103) v2cmowmbB685
CmoCh04G028650Watermelon (97103) v2cmowmbB772
CmoCh04G028650Wax gourdcmowgoB0836
CmoCh04G028650Cucurbita moschata (Rifu)cmocmoB123
CmoCh04G028650Cucurbita moschata (Rifu)cmocmoB185
CmoCh04G028650Cucurbita moschata (Rifu)cmocmoB261
CmoCh04G028650Cucumber (Gy14) v1cgycmoB0207
CmoCh04G028650Cucumber (Gy14) v1cgycmoB0442
CmoCh04G028650Cucumber (Gy14) v1cgycmoB0939
CmoCh04G028650Cucurbita maxima (Rimu)cmacmoB158
CmoCh04G028650Cucurbita maxima (Rimu)cmacmoB308
CmoCh04G028650Cucurbita maxima (Rimu)cmacmoB729
CmoCh04G028650Wild cucumber (PI 183967)cmocpiB690
CmoCh04G028650Wild cucumber (PI 183967)cmocpiB705
CmoCh04G028650Wild cucumber (PI 183967)cmocpiB722