CmoCh04G020350 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh04G020350
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Calcium-binding EF-hand family protein, putative
Location	Cmo_Chr04 : 11045870 .. 11046289 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAAGTATGAAGCAAGTGAGATGATAAAAAAGTGTACTAGAAATGGTGATAATGTTCAGCTAAAAACTTTTAGTGCCATTGCTCGAGCTTCAACTCAAGTCAACAATCAAGACATGGTACCGAAAATCTTTAGAGATAATAAGAATGTCGGGCCCAAGCTTAGTTCAAATACTCAACTAAAGACATCTAATATGCAATTTAGTCGAGAGCAGATGAAGGAGATTTTTAAATATCATGATAGCGACAAGGATGGTTTTCTCAACGTAAGGGAGCTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGACATGACAGATAAAGATAGCCTTATTAGCGAGGCTGAACTTGACAAACTCATTGATTATGTCCAGAAATTTATGAAGAAGAAATGA

mRNA sequence

ATGGCTAAGTATGAAGCAAGTGAGATGATAAAAAAGTGTACTAGAAATGGTGATAATGTTCAGCTAAAAACTTTTAGTGCCATTGCTCGAGCTTCAACTCAAGTCAACAATCAAGACATGGTACCGAAAATCTTTAGAGATAATAAGAATGTCGGGCCCAAGCTTAGTTCAAATACTCAACTAAAGACATCTAATATGCAATTTAGTCGAGAGCAGATGAAGGAGATTTTTAAATATCATGATAGCGACAAGGATGGTTTTCTCAACGTAAGGGAGCTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGACATGACAGATAAAGATAGCCTTATTAGCGAGGCTGAACTTGACAAACTCATTGATTATGTCCAGAAATTTATGAAGAAGAAATGA

Coding sequence (CDS)

ATGGCTAAGTATGAAGCAAGTGAGATGATAAAAAAGTGTACTAGAAATGGTGATAATGTTCAGCTAAAAACTTTTAGTGCCATTGCTCGAGCTTCAACTCAAGTCAACAATCAAGACATGGTACCGAAAATCTTTAGAGATAATAAGAATGTCGGGCCCAAGCTTAGTTCAAATACTCAACTAAAGACATCTAATATGCAATTTAGTCGAGAGCAGATGAAGGAGATTTTTAAATATCATGATAGCGACAAGGATGGTTTTCTCAACGTAAGGGAGCTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGACATGACAGATAAAGATAGCCTTATTAGCGAGGCTGAACTTGACAAACTCATTGATTATGTCCAGAAATTTATGAAGAAGAAATGA

Homology

[Close]

BLAST of CmoCh04G020350 vs. TrEMBL
Match: A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 2.8e-34
Identity = 89/163 (54.60%), Postives = 110/163 (67.48%), Query Frame = 1


Query: 2   AKYEASEMIKKCTRNGDNVQLKTFSAIARASTQVN-NQDMVPKIFRDNKNVGPKLSSNTQ 61
           AKYEAS+++KK  +NG+ V L  F+  AR STQ+N N++M  K  + N +V  KLSSN Q
Sbjct: 38  AKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQ 97

Query: 62  LKT--------------------SNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAFAFL 121
           LK+                    SNMQFSREQ+KEIF+Y+DSD+DGFLN+RE+TKAFA L
Sbjct: 98  LKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALL 157

Query: 122 GSIIPFEKASYDM----TDKDSLISEAELDKLIDYVQKFMKKK 140
           GSI PF KA + M    T+KD LI E ELDKLI+Y  KFMKKK
Sbjct: 158 GSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

[Close]

BLAST of CmoCh04G020350 vs. TrEMBL
Match: A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.3e-30
Identity = 87/165 (52.73%), Postives = 108/165 (65.45%), Query Frame = 1


Query: 2   AKYEASEMIKKCTRNGDNV----QLKTFSAIARASTQVNN-----QDMVPKIFRDNKNVG 61
           AK EA+E+IKK  RNGD V    +L++F   AR STQ+NN        VPK    N+N+ 
Sbjct: 10  AKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPKT---NENLA 69

Query: 62  PKLSSNTQL--------------KTSNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAFA 121
            K+SSNT +              K SN+  SREQ+KEIFKYHDS+KDGFL++RELTKAF+
Sbjct: 70  QKVSSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFS 129

Query: 122 FLGSIIPFEKASYDM----TDKDSLISEAELDKLIDYVQKFMKKK 140
            +GSI+PF KA Y M     D+D L+SEAELDKLIDY  K +KKK
Sbjct: 130 SMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]

BLAST of CmoCh04G020350 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 6.1e-29
Identity = 80/163 (49.08%), Postives = 103/163 (63.19%), Query Frame = 1


Query: 1   MAKYEASEMIKKCTRNGDNVQLKTFSAIARASTQVNNQDMVPKIFRDNKNVGPKLSSNTQ 60
           MA YEAS +IK+  +NGD V++K F  + R+STQ+ N++ V KI + +  V  KL S   
Sbjct: 10  MAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGVAHKLRSEAH 69

Query: 61  L--------------------KTSNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAFAFL 120
           +                    K+SNM  SRE++KEIF+YHD++ DGFLN  ELTKAFAFL
Sbjct: 70  IQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFL 129

Query: 121 GSIIPFEKASYDM----TDKDSLISEAELDKLIDYVQKFMKKK 140
           GS+ PF KA Y M     ++D LISEAELDKLIDY  K +KKK
Sbjct: 130 GSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]

BLAST of CmoCh04G020350 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.6e-27
Identity = 80/166 (48.19%), Postives = 102/166 (61.45%), Query Frame = 1


Query: 2   AKYEASEMIKKCTRNGDNVQLKTFSAIARASTQVNN-QDMVPKIF--RDNKNVGPKLSSN 61
           AKYEAS+++KK   N + V LK F+ I  AST++NN Q M  +I     N  V   L+SN
Sbjct: 11  AKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSNHPVAHYLTSN 70

Query: 62  T---------------------QLKTSNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAF 121
                                  +K SNMQFSREQ+KEIF+YHDSD DGFLN+RE+TKAF
Sbjct: 71  AYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAF 130

Query: 122 AFLGSIIPFEKASYDM----TDKDSLISEAELDKLIDYVQKFMKKK 140
           AFLG+I+P  KA + M     +KD LIS+ EL+KL+DY  KF  K+
Sbjct: 131 AFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmoCh04G020350 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.3e-14
Identity = 45/80 (56.25%), Postives = 55/80 (68.75%), Query Frame = 1


Query: 64  SNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAFAFLGSIIPFEKASYDMT----DKDSL 123
           S   FSRE+MKEIF+ HD D DG+L++ EL KA  FLG  IPF KA Y M     + D L
Sbjct: 7   SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 124 ISEAELDKLIDYVQKFMKKK 140
           ISE ELDKLIDY ++F K++
Sbjct: 67  ISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of CmoCh04G020350 vs. NCBI nr
Match: gi|700194512|gb|KGN49689.1| (hypothetical protein Csa_5G067670 [Cucumis sativus])

HSP 1 Score: 152.9 bits (385), Expect = 4.1e-34
Identity = 89/163 (54.60%), Postives = 110/163 (67.48%), Query Frame = 1


Query: 2   AKYEASEMIKKCTRNGDNVQLKTFSAIARASTQVN-NQDMVPKIFRDNKNVGPKLSSNTQ 61
           AKYEAS+++KK  +NG+ V L  F+  AR STQ+N N++M  K  + N +V  KLSSN Q
Sbjct: 38  AKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQ 97

Query: 62  LKT--------------------SNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAFAFL 121
           LK+                    SNMQFSREQ+KEIF+Y+DSD+DGFLN+RE+TKAFA L
Sbjct: 98  LKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALL 157

Query: 122 GSIIPFEKASYDM----TDKDSLISEAELDKLIDYVQKFMKKK 140
           GSI PF KA + M    T+KD LI E ELDKLI+Y  KFMKKK
Sbjct: 158 GSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

[Close]

BLAST of CmoCh04G020350 vs. NCBI nr
Match: gi|449470190|ref|XP_004152801.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus])

HSP 1 Score: 139.4 bits (350), Expect = 4.7e-30
Identity = 87/165 (52.73%), Postives = 108/165 (65.45%), Query Frame = 1


Query: 2   AKYEASEMIKKCTRNGDNV----QLKTFSAIARASTQVNN-----QDMVPKIFRDNKNVG 61
           AK EA+E+IKK  RNGD V    +L++F   AR STQ+NN        VPK    N+N+ 
Sbjct: 10  AKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPKT---NENLA 69

Query: 62  PKLSSNTQL--------------KTSNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAFA 121
            K+SSNT +              K SN+  SREQ+KEIFKYHDS+KDGFL++RELTKAF+
Sbjct: 70  QKVSSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFS 129

Query: 122 FLGSIIPFEKASYDM----TDKDSLISEAELDKLIDYVQKFMKKK 140
            +GSI+PF KA Y M     D+D L+SEAELDKLIDY  K +KKK
Sbjct: 130 SMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

[Close]

BLAST of CmoCh04G020350 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 135.2 bits (339), Expect = 8.8e-29
Identity = 80/163 (49.08%), Postives = 103/163 (63.19%), Query Frame = 1


Query: 1   MAKYEASEMIKKCTRNGDNVQLKTFSAIARASTQVNNQDMVPKIFRDNKNVGPKLSSNTQ 60
           MA YEAS +IK+  +NGD V++K F  + R+STQ+ N++ V KI + +  V  KL S   
Sbjct: 10  MAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGVAHKLRSEAH 69

Query: 61  L--------------------KTSNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAFAFL 120
           +                    K+SNM  SRE++KEIF+YHD++ DGFLN  ELTKAFAFL
Sbjct: 70  IQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFL 129

Query: 121 GSIIPFEKASYDM----TDKDSLISEAELDKLIDYVQKFMKKK 140
           GS+ PF KA Y M     ++D LISEAELDKLIDY  K +KKK
Sbjct: 130 GSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]

BLAST of CmoCh04G020350 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 3.7e-27
Identity = 80/166 (48.19%), Postives = 102/166 (61.45%), Query Frame = 1


Query: 2   AKYEASEMIKKCTRNGDNVQLKTFSAIARASTQVNN-QDMVPKIF--RDNKNVGPKLSSN 61
           AKYEAS+++KK   N + V LK F+ I  AST++NN Q M  +I     N  V   L+SN
Sbjct: 11  AKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSNHPVAHYLTSN 70

Query: 62  T---------------------QLKTSNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAF 121
                                  +K SNMQFSREQ+KEIF+YHDSD DGFLN+RE+TKAF
Sbjct: 71  AYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAF 130

Query: 122 AFLGSIIPFEKASYDM----TDKDSLISEAELDKLIDYVQKFMKKK 140
           AFLG+I+P  KA + M     +KD LIS+ EL+KL+DY  KF  K+
Sbjct: 131 AFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmoCh04G020350 vs. NCBI nr
Match: gi|659089539|ref|XP_008445564.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo])

HSP 1 Score: 129.4 bits (324), Expect = 4.8e-27
Identity = 82/161 (50.93%), Postives = 104/161 (64.60%), Query Frame = 1


Query: 2   AKYEASEMIKKCTRNGDNV----QLKTFSAIARAST-QVNNQDMVPKIFRDNKNVGPKLS 61
           AK EASE+IKK  RNGD V    +L++F   AR    +  +   +PK    N+N+  K+S
Sbjct: 10  AKIEASELIKKYDRNGDGVLTKEELQSFLHDARLKNIRFVSSSTIPKT---NENLDQKVS 69

Query: 62  SNTQLKT--------------SNMQFSREQMKEIFKYHDSDKDGFLNVRELTKAFAFLGS 121
           SNT++K               SN+  SREQ+KEIFKYHDS+KDGFL++RELTKAF  LGS
Sbjct: 70  SNTRIKPKKQLLDKAQDSLRQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFGSLGS 129

Query: 122 IIPFEKASYDM----TDKDSLISEAELDKLIDYVQKFMKKK 140
           I+PF KA + M     D+D LISEAEL KLIDY +K +KKK
Sbjct: 130 IVPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

The following BLAST results are available for this feature:

BLAST of CmoCh04G020350 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CmoCh04G020350 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLF3_CUCSA	2.8e-34	54.60	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1	[more]
A0A0A0LRC8_CUCSA	3.3e-30	52.73	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1	[more]
A0A0A0KMQ1_CUCSA	6.1e-29	49.08	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	2.6e-27	48.19	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	3.3e-14	56.25	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]

BLAST of CmoCh04G020350 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmoCh04G020350 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700194512\|gb\|KGN49689.1\|	4.1e-34	54.60	hypothetical protein Csa_5G067670 [Cucumis sativus]	[more]
gi\|449470190\|ref\|XP_004152801.1\|	4.7e-30	52.73	PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus]	[more]
gi\|700194510\|gb\|KGN49687.1\|	8.8e-29	49.08	hypothetical protein Csa_5G067410 [Cucumis sativus]	[more]
gi\|700194513\|gb\|KGN49690.1\|	3.7e-27	48.19	hypothetical protein Csa_5G068170 [Cucumis sativus]	[more]
gi\|659089539\|ref\|XP_008445564.1\|	4.8e-27	50.93	PREDICTED: probable calcium-binding protein CML11 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G020350.1	CmoCh04G020350.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 73..100 score: 2.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 68..103 score: 12
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 84..130 score: 5.2E-7coord: 7..83 score: 6.
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 64..125 score: 9.1
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 81..93 scor

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh04G020350	Cucumber (Gy14) v2	cgybcmoB645
CmoCh04G020350	Melon (DHL92) v3.6.1	cmomedB723
CmoCh04G020350	Melon (DHL92) v3.6.1	cmomedB746
CmoCh04G020350	Watermelon (97103) v2	cmowmbB699
CmoCh04G020350	Watermelon (97103) v2	cmowmbB712
CmoCh04G020350	Wax gourd	cmowgoB0906
CmoCh04G020350	Wax gourd	cmowgoB0914
CmoCh04G020350	Cucumber (Gy14) v1	cgycmoB0765
CmoCh04G020350	Cucurbita maxima (Rimu)	cmacmoB728
CmoCh04G020350	Wild cucumber (PI 183967)	cmocpiB741
CmoCh04G020350	Wild cucumber (PI 183967)	cmocpiB754
CmoCh04G020350	Cucumber (Chinese Long) v2	cmocuB745
CmoCh04G020350	Melon (DHL92) v3.5.1	cmomeB656
CmoCh04G020350	Watermelon (Charleston Gray)	cmowcgB623
CmoCh04G020350	Watermelon (97103) v1	cmowmB733
CmoCh04G020350	Cucurbita pepo (Zucchini)	cmocpeB673
CmoCh04G020350	Cucurbita pepo (Zucchini)	cmocpeB682
CmoCh04G020350	Cucurbita pepo (Zucchini)	cmocpeB686
CmoCh04G020350	Bottle gourd (USVL1VR-Ls)	cmolsiB623