CmoCh04G020330 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh04G020330
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Calcium-binding EF-hand family protein, putative
Location	Cmo_Chr04 : 11031682 .. 11032101 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAAGTATGAAGCAAGTGGGATGATGAAGAAGTATCCTAGAAATGGGGATGATGTTCAACTAAAATTATTCATGGTTCGAGATTCAACTCAAGTCAACAATCAAGGCAAGATATCGGAAATTCTAAAAGATAACAAGAATGTTGGGCTCAAGCTTAGTTCAACTACTTACCCCAAGACATCTAAGTTGCAATTTAGTCGAGAGCAGACGAAGGAGATCTTTAGATATCATGAGAGCGATAAGGATGGTTTTCTCAATTTAAGAGAGTTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATACCATTTGAGAAAGCTTCGTATGGCATGACATATGCTAATGTAAATAAAGATGGCCTTATTAGCGAGGCTGAACTTGACAAACTTGTTGATTATGCCCAGAAATTTATGACTTAG

mRNA sequence

ATGGCTAAGTATGAAGCAAGTGGGATGATGAAGAAGTATCCTAGAAATGGGGATGATGTTCAACTAAAATTATTCATGGTTCGAGATTCAACTCAAGTCAACAATCAAGGCAAGATATCGGAAATTCTAAAAGATAACAAGAATGTTGGGCTCAAGCTTAGTTCAACTACTTACCCCAAGACATCTAAGTTGCAATTTAGTCGAGAGCAGACGAAGGAGATCTTTAGATATCATGAGAGCGATAAGGATGGTTTTCTCAATTTAAGAGAGTTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATACCATTTGAGAAAGCTTCGTATGGCATGACATATGCTAATGTAAATAAAGATGGCCTTATTAGCGAGGCTGAACTTGACAAACTTGTTGATTATGCCCAGAAATTTATGACTTAG

Coding sequence (CDS)

ATGGCTAAGTATGAAGCAAGTGGGATGATGAAGAAGTATCCTAGAAATGGGGATGATGTTCAACTAAAATTATTCATGGTTCGAGATTCAACTCAAGTCAACAATCAAGGCAAGATATCGGAAATTCTAAAAGATAACAAGAATGTTGGGCTCAAGCTTAGTTCAACTACTTACCCCAAGACATCTAAGTTGCAATTTAGTCGAGAGCAGACGAAGGAGATCTTTAGATATCATGAGAGCGATAAGGATGGTTTTCTCAATTTAAGAGAGTTAACAAAAGCTTTTGCTTTTCTAGGCTCAATTATACCATTTGAGAAAGCTTCGTATGGCATGACATATGCTAATGTAAATAAAGATGGCCTTATTAGCGAGGCTGAACTTGACAAACTTGTTGATTATGCCCAGAAATTTATGACTTAG

Homology

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BLAST of CmoCh04G020330 vs. TrEMBL
Match: A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 9.1e-33
Identity = 85/160 (53.12%), Postives = 104/160 (65.00%), Query Frame = 1


Query: 2   AKYEASGMMKKYPRNGDDVQLKLFM--VRDSTQVN-NQGKISEILKDNKNVGLKLSSTTY 61
           AKYEAS +MKKY +NG+ V L  F    R STQ+N N+   ++ LK N +V  KLSS   
Sbjct: 38  AKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQ 97

Query: 62  --------------------PKTSKLQFSREQTKEIFRYHESDKDGFLNLRELTKAFAFL 121
                                K S +QFSREQ KEIF+Y++SD+DGFLN+RE+TKAFA L
Sbjct: 98  LKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALL 157

Query: 122 GSIIPFEKASYGMTYANVNKDGLISEAELDKLVDYAQKFM 139
           GSI PF KA +GM YA+ NKDGLI E ELDKL++YA KFM
Sbjct: 158 GSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFM 197

[Close]

BLAST of CmoCh04G020330 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 5.9e-32
Identity = 80/159 (50.31%), Postives = 104/159 (65.41%), Query Frame = 1


Query: 1   MAKYEASGMMKKYPRNGDDVQLKLFM-VRDSTQVNNQGKISEILKDNKNVGLKLSSTTY- 60
           MA YEAS ++K+Y +NGD V++K F  VR STQ+ N+  + +ILK +  V  KL S  + 
Sbjct: 10  MAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVAHKLRSEAHI 69

Query: 61  -------------------PKTSKLQFSREQTKEIFRYHESDKDGFLNLRELTKAFAFLG 120
                               K+S +  SRE+ KEIF+YH+++ DGFLN  ELTKAFAFLG
Sbjct: 70  QPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLG 129

Query: 121 SIIPFEKASYGMTYANVNKDGLISEAELDKLVDYAQKFM 139
           S+ PF KA YGM YA+ N+DGLISEAELDKL+DYA K +
Sbjct: 130 SMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKII 168

[Close]

BLAST of CmoCh04G020330 vs. TrEMBL
Match: A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.2e-30
Identity = 80/159 (50.31%), Postives = 107/159 (67.30%), Query Frame = 1


Query: 2   AKYEASGMMKKYPRNGDDV----QLKLFM--VRDSTQVNNQGKISE--ILKDNKNVGLKL 61
           AK EA+ ++KKY RNGD V    +L+ F+   R STQ+NN   +S   + K N+N+  K+
Sbjct: 10  AKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPKTNENLAQKV 69

Query: 62  SSTTY--------------PKTSKLQFSREQTKEIFRYHESDKDGFLNLRELTKAFAFLG 121
           SS T                K S +  SREQ KEIF+YH+S+KDGFL++RELTKAF+ +G
Sbjct: 70  SSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFSSMG 129

Query: 122 SIIPFEKASYGMTYANVNKDGLISEAELDKLVDYAQKFM 139
           SI+PF KA Y M YA+V++DGL+SEAELDKL+DYA K +
Sbjct: 130 SIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKII 168

[Close]

BLAST of CmoCh04G020330 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.6e-29
Identity = 83/162 (51.23%), Postives = 102/162 (62.96%), Query Frame = 1


Query: 2   AKYEASGMMKKYPRNGDDVQLKLF--MVRDSTQVNN-QGKISEIL--KDNKNVGLKLSST 61
           AKYEAS +MKK+  N + V LK F  +   ST++NN Q   +EI+    N  V   L+S 
Sbjct: 11  AKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSNHPVAHYLTSN 70

Query: 62  TYP---------------------KTSKLQFSREQTKEIFRYHESDKDGFLNLRELTKAF 121
            Y                      K S +QFSREQ KEIF+YH+SD DGFLNLRE+TKAF
Sbjct: 71  AYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAF 130

Query: 122 AFLGSIIPFEKASYGMTYANVNKDGLISEAELDKLVDYAQKF 138
           AFLG+I+P  KA +GM YA+ NKDGLIS+ EL+KLVDYA KF
Sbjct: 131 AFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKF 172

[Close]

BLAST of CmoCh04G020330 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 5.8e-19
Identity = 49/79 (62.03%), Postives = 58/79 (73.42%), Query Frame = 1


Query: 59  PKTSKLQFSREQTKEIFRYHESDKDGFLNLRELTKAFAFLGSIIPFEKASYGMTYANVNK 118
           P  SK  FSRE+ KEIFR H+ D DG+L++ EL KA  FLG  IPF KA YGM Y + N 
Sbjct: 4   PGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENG 63

Query: 119 DGLISEAELDKLVDYAQKF 138
           DGLISE ELDKL+DYA++F
Sbjct: 64  DGLISEDELDKLIDYAERF 82

[Close]

BLAST of CmoCh04G020330 vs. NCBI nr
Match: gi|700194512|gb|KGN49689.1| (hypothetical protein Csa_5G067670 [Cucumis sativus])

HSP 1 Score: 147.9 bits (372), Expect = 1.3e-32
Identity = 85/160 (53.12%), Postives = 104/160 (65.00%), Query Frame = 1


Query: 2   AKYEASGMMKKYPRNGDDVQLKLFM--VRDSTQVN-NQGKISEILKDNKNVGLKLSSTTY 61
           AKYEAS +MKKY +NG+ V L  F    R STQ+N N+   ++ LK N +V  KLSS   
Sbjct: 38  AKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQ 97

Query: 62  --------------------PKTSKLQFSREQTKEIFRYHESDKDGFLNLRELTKAFAFL 121
                                K S +QFSREQ KEIF+Y++SD+DGFLN+RE+TKAFA L
Sbjct: 98  LKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALL 157

Query: 122 GSIIPFEKASYGMTYANVNKDGLISEAELDKLVDYAQKFM 139
           GSI PF KA +GM YA+ NKDGLI E ELDKL++YA KFM
Sbjct: 158 GSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFM 197

[Close]

BLAST of CmoCh04G020330 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 145.2 bits (365), Expect = 8.5e-32
Identity = 80/159 (50.31%), Postives = 104/159 (65.41%), Query Frame = 1


Query: 1   MAKYEASGMMKKYPRNGDDVQLKLFM-VRDSTQVNNQGKISEILKDNKNVGLKLSSTTY- 60
           MA YEAS ++K+Y +NGD V++K F  VR STQ+ N+  + +ILK +  V  KL S  + 
Sbjct: 10  MAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVAHKLRSEAHI 69

Query: 61  -------------------PKTSKLQFSREQTKEIFRYHESDKDGFLNLRELTKAFAFLG 120
                               K+S +  SRE+ KEIF+YH+++ DGFLN  ELTKAFAFLG
Sbjct: 70  QPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLG 129

Query: 121 SIIPFEKASYGMTYANVNKDGLISEAELDKLVDYAQKFM 139
           S+ PF KA YGM YA+ N+DGLISEAELDKL+DYA K +
Sbjct: 130 SMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKII 168

[Close]

BLAST of CmoCh04G020330 vs. NCBI nr
Match: gi|449470190|ref|XP_004152801.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus])

HSP 1 Score: 139.0 bits (349), Expect = 6.1e-30
Identity = 80/159 (50.31%), Postives = 107/159 (67.30%), Query Frame = 1


Query: 2   AKYEASGMMKKYPRNGDDV----QLKLFM--VRDSTQVNNQGKISE--ILKDNKNVGLKL 61
           AK EA+ ++KKY RNGD V    +L+ F+   R STQ+NN   +S   + K N+N+  K+
Sbjct: 10  AKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPKTNENLAQKV 69

Query: 62  SSTTY--------------PKTSKLQFSREQTKEIFRYHESDKDGFLNLRELTKAFAFLG 121
           SS T                K S +  SREQ KEIF+YH+S+KDGFL++RELTKAF+ +G
Sbjct: 70  SSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFSSMG 129

Query: 122 SIIPFEKASYGMTYANVNKDGLISEAELDKLVDYAQKFM 139
           SI+PF KA Y M YA+V++DGL+SEAELDKL+DYA K +
Sbjct: 130 SIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKII 168

[Close]

BLAST of CmoCh04G020330 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 137.1 bits (344), Expect = 2.3e-29
Identity = 83/162 (51.23%), Postives = 102/162 (62.96%), Query Frame = 1


Query: 2   AKYEASGMMKKYPRNGDDVQLKLF--MVRDSTQVNN-QGKISEIL--KDNKNVGLKLSST 61
           AKYEAS +MKK+  N + V LK F  +   ST++NN Q   +EI+    N  V   L+S 
Sbjct: 11  AKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSNHPVAHYLTSN 70

Query: 62  TYP---------------------KTSKLQFSREQTKEIFRYHESDKDGFLNLRELTKAF 121
            Y                      K S +QFSREQ KEIF+YH+SD DGFLNLRE+TKAF
Sbjct: 71  AYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAF 130

Query: 122 AFLGSIIPFEKASYGMTYANVNKDGLISEAELDKLVDYAQKF 138
           AFLG+I+P  KA +GM YA+ NKDGLIS+ EL+KLVDYA KF
Sbjct: 131 AFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKF 172

[Close]

BLAST of CmoCh04G020330 vs. NCBI nr
Match: gi|659089539|ref|XP_008445564.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo])

HSP 1 Score: 128.3 bits (321), Expect = 1.1e-26
Identity = 77/155 (49.68%), Postives = 99/155 (63.87%), Query Frame = 1


Query: 2   AKYEASGMMKKYPRNGDDVQLK--LFMVRDSTQVNNQGKISE--ILKDNKNVGLKLSSTT 61
           AK EAS ++KKY RNGD V  K  L       ++ N   +S   I K N+N+  K+SS T
Sbjct: 10  AKIEASELIKKYDRNGDGVLTKEELQSFLHDARLKNIRFVSSSTIPKTNENLDQKVSSNT 69

Query: 62  YPKTSK--------------LQFSREQTKEIFRYHESDKDGFLNLRELTKAFAFLGSIIP 121
             K  K              +  SREQ KEIF+YH+S+KDGFL++RELTKAF  LGSI+P
Sbjct: 70  RIKPKKQLLDKAQDSLRQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFGSLGSIVP 129

Query: 122 FEKASYGMTYANVNKDGLISEAELDKLVDYAQKFM 139
           F KA + M YA+V++DGLISEAEL KL+DYA+K +
Sbjct: 130 FAKARHAMAYADVDEDGLISEAELHKLIDYAEKII 164

The following BLAST results are available for this feature:

BLAST of CmoCh04G020330 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CmoCh04G020330 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLF3_CUCSA	9.1e-33	53.13	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1	[more]
A0A0A0KMQ1_CUCSA	5.9e-32	50.31	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1	[more]
A0A0A0LRC8_CUCSA	4.2e-30	50.31	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	1.6e-29	51.23	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	5.8e-19	62.03	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]

BLAST of CmoCh04G020330 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmoCh04G020330 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700194512\|gb\|KGN49689.1\|	1.3e-32	53.13	hypothetical protein Csa_5G067670 [Cucumis sativus]	[more]
gi\|700194510\|gb\|KGN49687.1\|	8.5e-32	50.31	hypothetical protein Csa_5G067410 [Cucumis sativus]	[more]
gi\|449470190\|ref\|XP_004152801.1\|	6.1e-30	50.31	PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus]	[more]
gi\|700194513\|gb\|KGN49690.1\|	2.3e-29	51.23	hypothetical protein Csa_5G068170 [Cucumis sativus]	[more]
gi\|659089539\|ref\|XP_008445564.1\|	1.1e-26	49.68	PREDICTED: probable calcium-binding protein CML11 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G020330.1	CmoCh04G020330.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 116..137 score: 5.671coord: 66..101 score: 10
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 60..131 score: 5.
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 62..129 score: 4.28

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh04G020330	Watermelon (97103) v2	cmowmbB699
CmoCh04G020330	Watermelon (97103) v2	cmowmbB712
CmoCh04G020330	Wax gourd	cmowgoB0906
CmoCh04G020330	Wax gourd	cmowgoB0914
CmoCh04G020330	Cucumber (Gy14) v1	cgycmoB0765
CmoCh04G020330	Cucurbita maxima (Rimu)	cmacmoB728
CmoCh04G020330	Wild cucumber (PI 183967)	cmocpiB741
CmoCh04G020330	Wild cucumber (PI 183967)	cmocpiB754
CmoCh04G020330	Cucumber (Chinese Long) v2	cmocuB745
CmoCh04G020330	Melon (DHL92) v3.5.1	cmomeB656
CmoCh04G020330	Watermelon (Charleston Gray)	cmowcgB623
CmoCh04G020330	Watermelon (97103) v1	cmowmB733
CmoCh04G020330	Cucurbita pepo (Zucchini)	cmocpeB673
CmoCh04G020330	Cucurbita pepo (Zucchini)	cmocpeB682
CmoCh04G020330	Cucurbita pepo (Zucchini)	cmocpeB686
CmoCh04G020330	Bottle gourd (USVL1VR-Ls)	cmolsiB623
CmoCh04G020330	Cucumber (Gy14) v2	cgybcmoB645
CmoCh04G020330	Melon (DHL92) v3.6.1	cmomedB723
CmoCh04G020330	Melon (DHL92) v3.6.1	cmomedB746