CmoCh04G015260 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh04G015260
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	3-oxo-5-alpha-steroid 4-dehydrogenase family protein
Location	Cmo_Chr04 : 7807316 .. 7810176 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATGGTTGCGATCTATAAATCCTCATCTTCAACGCCTCCAAAGGCGTTTCAAAATTCTACAGAGAGTTTCTGGCGGTTTTTCTCATCTGGTTCTCGGCGGCGATGGGTATTTTGTCGGCGTTTCTTTTCCCACCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCAGGGATTTCAGAGATTAATGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTTCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTCGAGGTACTCTCAATTTCTCTACGCCTCTGATTGAGTTCTGAAATTTCAATCCGATTTTAAAATTAATTCTTCGATTCAGTTCGATTGTGGCTTTATTGAGAACCTGTTAATCTTATTCTTCGAAAATTTCTCGAGTCCTTTATACAACTGTATGAAGATTCGTGGCGAAAAGAAAATGGCGGAGAGATCTGGATCTCTTCTCCCTTCTTTGCATCTTATGGTTCCTCTGGAGATTTCGGAACTCGTCTTTATGTTTACGGGATTCGGAAGACTTTTAACCTATCTTGATTTCTTTTTTTTAATTTTTTGTCGTATGAACTTGAATATTAACCATCCACTTATAAATATCCGAGTTCATTAGTTTTCCTCTACTAATTTAATTGTCATTAAGAACTCGTGGAGTCTACTTTGTGTATAATAATAATTTTGGAATTATTATTATATGAAGTCATGTACAAGAAAGAACAAACTGCACGTTTCTGCTCAACAGTTTTCTAAGAAATTTGTTTCAATAATCACTAGATGATTTGCACTTATTGTACCTTTCATTCATGCGACAATTATTTTTGAAACAAATCGAGTATTCTTCTCAAATTATAGAAGCTATTCTTTTATTATTTCTTCAACCAATTGCATCTGAGGGTAACTAGAAATGATAACAAAAGTTTTGGAAGATCATATGAATCATTTAGAGAACAATGAAAAGTAATATAAACAAACATTTTTTCAGAAACAACAAAACACCTAATCTTATATTAAATATCATTGAATTCAAAAGCTCAAACGGATAGACTATATGAATTACTTACTATCATGAAATTCTTTGCAGGTTCTGTTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTTGTCCACAGTGATCATGATATACACACAGCACCTATCACAAGGACTTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCATTTTCTCTTGTCCAAAACCAGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCCTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGGTACGTCTCCAAATTTGAAAACTTCCCCAGCCATGTCAAAGCCCTGCTCCCTTTTGTGTTTTAGAACCCTTCCTTTCTTCATTTCATAAAAGCTACTTATGTTTATGCTTTACACAATTAAGTTAGGCTCATACTACTATTGTATTAGTTCCCTTAGGTTGTGATTCTGTTTATCACTACAATAGTAGAAGGTAAAACAGTTGGATATTTGTTCTTTACCTGTTCTTAATAACGACGATTTATACTTGTGTGTTTTTATTTATTATAATTAAAATAGTTTTTATGTTATGAAATTGAATTGAATTAAATTAAATTAAAAATGTTATTGGCAAAAATTAGTCTAGAATTCCTAGGTTATATATTTGTTGTTTTAGAAAACCGCTCGTTCATGCAAGTGTTTCTATGGACTTCGTCGACCAAATTGGGTTGTTCGCATTTTTAGGCCGTAGTATAGAAGAGGAGTATTTAAAAAAATTCGATAATCTGAAAAGTTCTATTAATCCAATCCAAACCTATACTTAAAAATGTTTAAACAAAGAATTGAATAAATAATTCGAATGGGCATAAAAAAAGAAAAATTTATAGTTAGGTTCATTATTTTAAAAAACAAATTACACAATTGAAATAATTTAGTTGAGTTTAAAATTTTTTTCAGGATCAATCTAATCCAACCCATTAATACATGGAGGAGGAGGGAAAAGAGAAAAACCTTCTAATTTTTTTTAAAAAAAACTAATTTCTTAGAAAATAAATGTGTAAATTTTGTTTACTCTTTTTTTTATAAAAAAAAAATTAAAATTAGGAGGATAAAAATTGTTATTTTCTAGATTTATTTAATTTTTTATTTATTAAATAACTTTAAACTTATAAATCTTTTTCAAAACATTTTTATTCATAAAATATCTGGACAATAGGCTCTGATTTTTTTTTTTATAAATAAGGTTGCAAACTATCAAAAATAAAAATATTTTTAGAATATACAGCTAAAATAAATCATAAATAATACTAGAAAAAGAAAAAAACAATAAAAAAAACAAGAAATGTATTATATATATATATTACTTAATTAAGTAATGGCCACACACTAAGAAAGAAAAGGACATGGAGATTTTTTTTTAATAAATTGCAGAGCGATGAGGCAACCGTCGTTCACCATCAAATTTCCATCCTTCTCTGCAACTCACGATTTCAAGA

mRNA sequence

AAAATGGTTGCGATCTATAAATCCTCATCTTCAACGCCTCCAAAGGCGTTTCAAAATTCTACAGAGAGTTTCTGGCGGTTTTTCTCATCTGGTTCTCGGCGGCGATGGGTATTTTGTCGGCGTTTCTTTTCCCACCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCAGGGATTTCAGAGATTAATGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTTCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTCGAGGTTCTGTTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTTGTCCACAGTGATCATGATATACACACAGCACCTATCACAAGGACTTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCATTTTCTCTTGTCCAAAACCAGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCCTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGA

Coding sequence (CDS)

ATGGGTATTTTGTCGGCGTTTCTTTTCCCACCGCCTCCTTCCACGTTCGTAAACGTTATGACGGTGGTGAGCTCGACGGCGATGGGGATGGCAGGGATTTCAGAGATTAATGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATAGTTCTCCGAAAGCTCCATCGGAGGAACCTAAGCTTTCGTCCAAATATGGAATGCTCTTGGCTTATACGCCGGCATTTCTCGCCGGTGCGGCGTCGTTTTGGTTCTTTCCAAGCGACAATGAGAGGATTCTTGTGCTTAAAGCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTCGAGGTTCTGTTCATTCACAAGTACAGCAGTAAAATGGTATTCGATACAGCAATCAAAATCTCGTTCAGCTACTGCTTGTCCACAGTGATCATGATATACACACAGCACCTATCACAAGGACTTCCAGAGCCGCCCATTGATCTGAAAAACATTGGGATTGCTTTGTTTCTGATAGGCATTATCGGGAACTTCTACCACCATTTTCTCTTGTCCAAAACCAGAAAACCGGGAGAAACAGGGTACAAAATCCCCAAGGGAGGCCTCTTCAGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATCATTGAGTTTTTTGGATTTGCTTTCATCTCTCAGGAACTCTATCCCCTGTGCTTCAGCACCGGCACCGCCATTTATTTGGCTGGAAGGAGCTATGCCACAAGGAAATGA

Homology

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BLAST of CmoCh04G015260 vs. Swiss-Prot
Match: S5A1_RAT (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 2.9e-11
Identity = 35/80 (43.75%), Postives = 49/80 (61.25%), Query Frame = 1


Query: 161 GIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFIS 220
           G AL+L+G++ N +   +L   RKPGETGYKIP+GGLF  V   +Y  E++E+ GFA  S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213

Query: 221 QELYPLCFSTGTAIYLAGRS 241
             L  + F+  T   L  R+
Sbjct: 214 WSLQGVVFALFTLSTLLTRA 233

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BLAST of CmoCh04G015260 vs. Swiss-Prot
Match: S5A1_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.8e-11
Identity = 34/81 (41.98%), Postives = 50/81 (61.73%), Query Frame = 1


Query: 160 IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFI 219
           IG  L+L G++ N +   +L   RKPG+TGYKIP+GGLF  V   +Y  EI+E+ G+A  
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 220 SQELYPLCFSTGTAIYLAGRS 241
           S  +    F+  T  +L+GR+
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRA 237

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BLAST of CmoCh04G015260 vs. Swiss-Prot
Match: S5A1_HUMAN (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.5e-11
Identity = 46/148 (31.08%), Postives = 73/148 (49.32%), Query Frame = 1


Query: 95  VLKAALTLHFFKRIFEVLFIHKYSSKM-VFDTAIKISFSYCLSTVIMIYTQHLSQGLPEP 154
           +L A   +H+  R     F+ +    M +    + I F  C   +   Y  H +    + 
Sbjct: 86  ILLAMFLVHYGHRCLIYPFLMRGGKPMPLLACTMAIMFCTCNGYLQSRYLSHCAVYADDW 145

Query: 155 PIDLKN-IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIE 214
             D +  IG  L+L G++ N +   +L   RKPG+TGYKIP+GGLF  V   +Y  EI+E
Sbjct: 146 VTDPRFLIGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIME 205

Query: 215 FFGFAFISQELYPLCFSTGTAIYLAGRS 241
           + G+A  S  +    F+  T  +L+GR+
Sbjct: 206 WCGYALASWSVQGAAFAFFTFCFLSGRA 233

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BLAST of CmoCh04G015260 vs. Swiss-Prot
Match: S5A1_MOUSE (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 8.5e-11
Identity = 62/223 (27.80%), Postives = 99/223 (44.39%), Query Frame = 1


Query: 28  MGMAGISEINGKHLQYSKFWNANSSPKAPS----EEPKLSSKYGMLLAYTPAFLAGAASF 87
           +   G+  +   + +YS  W+    P  P+    E P ++  + +     PA    AA  
Sbjct: 22  VAFVGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMA--WPLYECIRPA----AARL 81

Query: 88  WFFPSDNERILVLKAALTLHFFKR--IFEVLFIHKYSSKMVFDTAIKISF----SYCLST 147
              P+      VL A   +H+ +R  +F VL I      ++F   +   F     Y  S 
Sbjct: 82  GNLPNR-----VLLAMFLIHYVQRTLVFPVL-IRGGKPTLLFTFVLAFLFCTLNGYLQSR 141

Query: 148 VIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGL 207
            +  +  +    +  P       G AL+L+G++ N +   +L   RKPGETGYKIP+GGL
Sbjct: 142 YLSQFAVYAEDWVTHPCF---LTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGL 201

Query: 208 FSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 241
           F  V   +Y  E++E+ GFA  S  L  + F+  T   L  R+
Sbjct: 202 FEYVSSANYFGELVEWCGFALASWSLQGVVFALFTLCALFTRA 229

[Close]

BLAST of CmoCh04G015260 vs. Swiss-Prot
Match: S5A2_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.9e-10
Identity = 45/151 (29.80%), Postives = 80/151 (52.98%), Query Frame = 1


Query: 95  VLKAALTLHFFKRIFEVLFIHK---YSSKMVFDTAIKISFSYCLSTVIMIYTQHLSQGLP 154
           VL     +H+F R F    +++   Y + ++F      + +  L +  +IY      G  
Sbjct: 82  VLLGLFCVHYFHRTFVYSLLNRGRPYPAVLIFRGIAFCAGNGFLQSYYLIYCAEYPDGWY 141

Query: 155 EPPIDLKN-IGIALFLIGIIGNFYHHFLLSKTRKPGETGYKIPKGGLFSLVICPHYLFEI 214
               D++  +G+ LF++G+  N +  ++L + RKPGE  Y+IPKGGLF+ V   ++L EI
Sbjct: 142 T---DIRFCLGVFLFILGMGVNIHSDYILRQLRKPGEITYRIPKGGLFTYVSGANFLGEI 201

Query: 215 IEFFGFAFISQELYPLCFSTGTAIYLAGRSY 242
           IE+ G+A  +  L  L F+  +  +L  R++
Sbjct: 202 IEWIGYALATWSLPALAFAFFSVCFLGLRAF 229

[Close]

BLAST of CmoCh04G015260 vs. TrEMBL
Match: A0A0A0KTC7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612880 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 3.3e-102
Identity = 189/245 (77.14%), Postives = 206/245 (84.08%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN + K  +EE 
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFW FPS +ER                  VLFIHKYSSK
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDER------------------VLFIHKYSSK 140

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV  TAI+IS SY +ST IMIYTQHLSQGLPEPPIDLKNIG+  FLIGIIGNFYHH+LLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           +TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 247

Query: 241 YATRK 246
           YATRK
Sbjct: 261 YATRK 247

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BLAST of CmoCh04G015260 vs. TrEMBL
Match: A0A0A0KWK8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612900 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 2.6e-91
Identity = 173/246 (70.33%), Postives = 200/246 (81.30%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M  LS F+FPPP S FVNVMTVVSS  + + G+SE  GKHL+YSKFW++N++     +  
Sbjct: 1   MDTLSEFVFPPPASAFVNVMTVVSSAIVAVVGLSEARGKHLKYSKFWSSNNA-NTDRKSF 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
            LS+K GML+AY PAFLAG  S W FPS++ RIL+LK+AL LHFFKR  EVLF+HKYSSK
Sbjct: 61  NLSAKLGMLVAYIPAFLAGVVSLWLFPSEDRRILLLKSALVLHFFKRNLEVLFLHKYSSK 120

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGL-PEPPIDLKNIGIALFLIGIIGNFYHHFLL 180
           M  D+ I ISFSY  ST IMIYTQHLSQGL  EPPIDLK+IG+ LFLIGIIGNFYHH+LL
Sbjct: 121 MAVDSVITISFSYFSSTAIMIYTQHLSQGLLREPPIDLKSIGVGLFLIGIIGNFYHHYLL 180

Query: 181 SKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGR 240
           S+TRK GET YKIPKGGLFSL+ICPHYLFEIIEFFGFAFISQ +Y L F+  TA+YL+GR
Sbjct: 181 SQTRKQGETSYKIPKGGLFSLIICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLSGR 240

Query: 241 SYATRK 246
           SYATRK
Sbjct: 241 SYATRK 245

[Close]

BLAST of CmoCh04G015260 vs. TrEMBL
Match: A0A0A0KRA7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612890 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.3e-87
Identity = 167/247 (67.61%), Postives = 194/247 (78.54%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWN-ANSSPKAP-SE 60
           M  LSAF FP PPS  VNVMTVVSS  + + G SE  G HLQYSKFWN +NS+ K+  SE
Sbjct: 7   MNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSE 66

Query: 61  EPKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYS 120
           + ++SS+ GMLL YTPA LAGAASFWF P+D+ R+L+LK+ALT HFFKR FEVLF+HKYS
Sbjct: 67  QLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKYS 126

Query: 121 SKMVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFL 180
            KMV D+A+ IS SY  ST +MIY Q  S+ LPEP IDLKNIGIA+F+IGIIGN YHH L
Sbjct: 127 GKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHIL 186

Query: 181 LSKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAG 240
           LS+TRK GETGYKIPKGGLF ++ICPHY+FEI  +FGFA ISQ  Y   F+  TAIYL G
Sbjct: 187 LSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLGG 246

Query: 241 RSYATRK 246
           RSYATRK
Sbjct: 247 RSYATRK 253

[Close]

BLAST of CmoCh04G015260 vs. TrEMBL
Match: I1JDE1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G081200 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.1e-84
Identity = 159/245 (64.90%), Postives = 188/245 (76.73%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M  L +F+FPPP S  V+ M+V+S  ++  AG SEI GKHL YSKFWNAN S +   ++ 
Sbjct: 4   MSALFSFIFPPPSSLVVSGMSVISLVSLANAGFSEIRGKHLNYSKFWNANPSAE---KQV 63

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSK GMLL YTPAFLAG ASFW FP    R  +L++A+TLHFFKR+FEV+FIHKYS  
Sbjct: 64  KLSSKAGMLLLYTPAFLAGLASFWIFPHQGLRSTLLQSAVTLHFFKRVFEVVFIHKYSGA 123

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           M+ D+AI I+ SY LST  MIY QHL+QGLPEPPIDL   GI LF++GIIGNFYHH+LLS
Sbjct: 124 MLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPIDLLYPGIVLFVVGIIGNFYHHYLLS 183

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
             R  GE  YKIPKGG+F LVICPHYLFEIIEF+GF+FISQ LY   F+ GT +YL GRS
Sbjct: 184 NLRGKGEKEYKIPKGGMFELVICPHYLFEIIEFYGFSFISQTLYAFSFTVGTTLYLLGRS 243

Query: 241 YATRK 246
           Y+TRK
Sbjct: 244 YSTRK 245

[Close]

BLAST of CmoCh04G015260 vs. TrEMBL
Match: A0A0S3TAT5_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.11G143800 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 9.0e-84
Identity = 158/245 (64.49%), Postives = 185/245 (75.51%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M    +F+FPPPPS  V+ M+V+S  ++  AG+SEI GKHL YSKFWN N S +   ++ 
Sbjct: 3   MSTFLSFIFPPPPSLVVSTMSVISLVSLANAGLSEIRGKHLNYSKFWNVNPSSE---KQT 62

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSK GMLL YTPAFLAG ASFW FP +  R  +L+ A+TLHFFKR FEVLFIHKYS  
Sbjct: 63  KLSSKAGMLLLYTPAFLAGLASFWIFPHEGLRSTLLQFAVTLHFFKRDFEVLFIHKYSGG 122

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           M+ D+AI IS SY LS   M+Y QHL+QGLPEPPI+L   GI +F+IGIIGNFYHH+LLS
Sbjct: 123 MLLDSAIPISLSYFLSAATMVYAQHLTQGLPEPPINLLYPGIVMFVIGIIGNFYHHYLLS 182

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
             R  GE  YKIPKGGLF +VICPHYLFEII+F+G  FISQ LY LCFS GT  YL GRS
Sbjct: 183 NLRAKGEKEYKIPKGGLFGIVICPHYLFEIIQFYGITFISQTLYGLCFSLGTTFYLLGRS 242

Query: 241 YATRK 246
           Y+TRK
Sbjct: 243 YSTRK 244

[Close]

BLAST of CmoCh04G015260 vs. TAIR10
Match: AT5G16010.1 (AT5G16010.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 275.0 bits (702), Expect = 4.4e-74
Identity = 138/246 (56.10%), Postives = 181/246 (73.58%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEE- 60
           M ++++F++PPPPS  +N M+VV   A+   G SEI G HL+YSKF  ++SSP+   E  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 61  PKLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSS 120
             +SS+ GMLL YTPAFLA A+SF+  PSD+ R L+LK+AL LHFFKR+FEVLFIHKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 121 KMVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLL 180
            M  D+A+ IS SY  ST +M+Y+Q+L+ GL EP  D+K  G+ +F++GI+GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 181 SKTRK-PGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAG 240
           +K RK  G+  YKIPKGGLF ++ICPHYLFEI+ F+ F  ISQ +Y   F+ GT +YL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 241 RSYATR 245
           RSYATR
Sbjct: 241 RSYATR 246

[Close]

BLAST of CmoCh04G015260 vs. NCBI nr
Match: gi|778706250|ref|XP_004135052.2| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus])

HSP 1 Score: 418.3 bits (1074), Expect = 9.2e-114
Identity = 203/245 (82.86%), Postives = 223/245 (91.02%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN + K  +EE 
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 140

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV  TAI+IS SY +ST IMIYTQHLSQGLPEPPIDLKNIG+  FLIGIIGNFYHH+LLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           +TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260

Query: 241 YATRK 246
           YATRK
Sbjct: 261 YATRK 265

[Close]

BLAST of CmoCh04G015260 vs. NCBI nr
Match: gi|659091769|ref|XP_008446722.1| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo])

HSP 1 Score: 417.2 bits (1071), Expect = 2.0e-113
Identity = 207/245 (84.49%), Postives = 220/245 (89.80%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M ILSAFLFPPPPSTFVNVMTVVSST++ MAG+SEI GKHLQYSKFWNANS  K  ++E 
Sbjct: 11  MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLL YTPAFLAGAASFW FPS +ER  +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 71  KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 130

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV  TAI+ISFSY LST IMIYTQHLSQGLPEPPIDLKNIG+ LFL GIIGNFYHH+LLS
Sbjct: 131 MVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHYLLS 190

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           +TRK  ET YKIPKGGLFS VICPHYLFEII +FGFAFISQELYPLCFS GTAIYLAGRS
Sbjct: 191 QTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 250

Query: 241 YATRK 246
           YATRK
Sbjct: 251 YATRK 255

[Close]

BLAST of CmoCh04G015260 vs. NCBI nr
Match: gi|700196982|gb|KGN52159.1| (hypothetical protein Csa_5G612880 [Cucumis sativus])

HSP 1 Score: 379.4 bits (973), Expect = 4.7e-102
Identity = 189/245 (77.14%), Postives = 206/245 (84.08%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN + K  +EE 
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFW FPS +ER                  VLFIHKYSSK
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDER------------------VLFIHKYSSK 140

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180
           MV  TAI+IS SY +ST IMIYTQHLSQGLPEPPIDLKNIG+  FLIGIIGNFYHH+LLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200

Query: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240
           +TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 247

Query: 241 YATRK 246
           YATRK
Sbjct: 261 YATRK 247

[Close]

BLAST of CmoCh04G015260 vs. NCBI nr
Match: gi|659091773|ref|XP_008446724.1| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo])

HSP 1 Score: 344.0 bits (881), Expect = 2.2e-91
Identity = 172/246 (69.92%), Postives = 195/246 (79.27%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M  LS F+FPPP S FVNVMTVVSS  + + G+SE  GKHLQYSKFWN N++     +  
Sbjct: 1   MDALSEFVFPPPSSAFVNVMTVVSSAIVAVVGLSEARGKHLQYSKFWNNNNNANTDRKSF 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
            LS+K GMLLAY PAFL G  SFW FP+++ RI+VLK+AL LHFFKR  EVLF+HKYSSK
Sbjct: 61  NLSAKLGMLLAYMPAFLVGVVSFWLFPNEDRRIVVLKSALVLHFFKRNLEVLFLHKYSSK 120

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQG-LPEPPIDLKNIGIALFLIGIIGNFYHHFLL 180
           M   + I ISFSY  ST IMIYTQHLSQG LPEPPIDLK IG+ LFLIGI+GNFYHH+LL
Sbjct: 121 MAIGSVITISFSYFSSTAIMIYTQHLSQGLLPEPPIDLKIIGVGLFLIGIVGNFYHHYLL 180

Query: 181 SKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGR 240
           S TRK GET YKIP+GGLF+ VICPHYLFEIIEFFGFAFISQ +Y L F+  TA+YL GR
Sbjct: 181 SGTRKGGETSYKIPEGGLFNFVICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLTGR 240

Query: 241 SYATRK 246
           SYATRK
Sbjct: 241 SYATRK 246

[Close]

BLAST of CmoCh04G015260 vs. NCBI nr
Match: gi|449434532|ref|XP_004135050.1| (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus])

HSP 1 Score: 343.2 bits (879), Expect = 3.8e-91
Identity = 173/246 (70.33%), Postives = 200/246 (81.30%), Query Frame = 1


Query: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60
           M  LS F+FPPP S FVNVMTVVSS  + + G+SE  GKHL+YSKFW++N++     +  
Sbjct: 1   MDTLSEFVFPPPASAFVNVMTVVSSAIVAVVGLSEARGKHLKYSKFWSSNNA-NTDRKSF 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120
            LS+K GML+AY PAFLAG  S W FPS++ RIL+LK+AL LHFFKR  EVLF+HKYSSK
Sbjct: 61  NLSAKLGMLVAYIPAFLAGVVSLWLFPSEDRRILLLKSALVLHFFKRNLEVLFLHKYSSK 120

Query: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGL-PEPPIDLKNIGIALFLIGIIGNFYHHFLL 180
           M  D+ I ISFSY  ST IMIYTQHLSQGL  EPPIDLK+IG+ LFLIGIIGNFYHH+LL
Sbjct: 121 MAVDSVITISFSYFSSTAIMIYTQHLSQGLLREPPIDLKSIGVGLFLIGIIGNFYHHYLL 180

Query: 181 SKTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGR 240
           S+TRK GET YKIPKGGLFSL+ICPHYLFEIIEFFGFAFISQ +Y L F+  TA+YL+GR
Sbjct: 181 SQTRKQGETSYKIPKGGLFSLIICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLSGR 240

Query: 241 SYATRK 246
           SYATRK
Sbjct: 241 SYATRK 245

The following BLAST results are available for this feature:

BLAST of CmoCh04G015260 vs. Swiss-Prot


Match Name	E-value	Identity	Description
S5A1_RAT	2.9e-11	43.75	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2	[more]
S5A1_MACFA	3.8e-11	41.98	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV...	[more]
S5A1_HUMAN	8.5e-11	31.08	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1	[more]
S5A1_MOUSE	8.5e-11	27.80	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2	[more]
S5A2_MACFA	1.9e-10	29.80	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis GN=SRD5A2 PE=1 SV...	[more]

BLAST of CmoCh04G015260 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KTC7_CUCSA	3.3e-102	77.14	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612880 PE=4 SV=1	[more]
A0A0A0KWK8_CUCSA	2.6e-91	70.33	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612900 PE=4 SV=1	[more]
A0A0A0KRA7_CUCSA	2.3e-87	67.61	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612890 PE=4 SV=1	[more]
I1JDE1_SOYBN	3.1e-84	64.90	Uncharacterized protein OS=Glycine max GN=GLYMA_02G081200 PE=4 SV=1	[more]
A0A0S3TAT5_PHAAN	9.0e-84	64.49	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.11G143800 PE=...	[more]

BLAST of CmoCh04G015260 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16010.1	4.4e-74	56.10	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

BLAST of CmoCh04G015260 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778706250\|ref\|XP_004135052.2\|	9.2e-114	82.86	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus]	[more]
gi\|659091769\|ref\|XP_008446722.1\|	2.0e-113	84.49	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo]	[more]
gi\|700196982\|gb\|KGN52159.1\|	4.7e-102	77.14	hypothetical protein Csa_5G612880 [Cucumis sativus]	[more]
gi\|659091773\|ref\|XP_008446724.1\|	2.2e-91	69.92	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo]	[more]
gi\|449434532\|ref\|XP_004135050.1\|	3.8e-91	70.33	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001104	3-oxo-5_a-steroid_4-DH_C

Vocabulary: **Molecular Function**
Term	Definition
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm
GO:0016021	integral component of membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G015260.1	CmoCh04G015260.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 157..240 score: 7.2
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROFILE	PS50244	S5A_REDUCTASE	coord: 161..228 score: 25
None	No IPR available	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 16..245 score: 2.0E
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 16..245 score: 2.0E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh04G015260	Watermelon (97103) v2	cmowmbB729
CmoCh04G015260	Wax gourd	cmowgoB0897
CmoCh04G015260	Cucurbita moschata (Rifu)	cmocmoB336
CmoCh04G015260	Cucumber (Gy14) v1	cgycmoB0793
CmoCh04G015260	Cucurbita maxima (Rimu)	cmacmoB420
CmoCh04G015260	Cucurbita maxima (Rimu)	cmacmoB728
CmoCh04G015260	Wild cucumber (PI 183967)	cmocpiB745
CmoCh04G015260	Cucumber (Chinese Long) v2	cmocuB738
CmoCh04G015260	Melon (DHL92) v3.5.1	cmomeB631
CmoCh04G015260	Watermelon (Charleston Gray)	cmowcgB654
CmoCh04G015260	Watermelon (97103) v1	cmowmB662
CmoCh04G015260	Watermelon (97103) v1	cmowmB693
CmoCh04G015260	Cucurbita pepo (Zucchini)	cmocpeB651
CmoCh04G015260	Cucurbita pepo (Zucchini)	cmocpeB673
CmoCh04G015260	Bottle gourd (USVL1VR-Ls)	cmolsiB660
CmoCh04G015260	Cucumber (Gy14) v2	cgybcmoB650
CmoCh04G015260	Melon (DHL92) v3.6.1	cmomedB720
CmoCh04G015260	Silver-seed gourd	carcmoB1138
CmoCh04G015260	Silver-seed gourd	carcmoB1412
CmoCh04G015260	Cucumber (Chinese Long) v3	cmocucB0874