CmoCh04G009910 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh04G009910
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionCalcium-binding EF-hand family protein, putative
LocationCmo_Chr04 : 4990791 .. 4991288 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCGCAAAACCAAAACGTACCACCCGAGGCCCAGGATATCAAGGGAGCAATTGAGGCTAATCTTCAGAAACCACGACGACAACGGCGACGGCCGCCTCAGCTCCGCCGAGCTCACCCAAGCCTTCCACTATATCGGCGCCCTTTTGCCATCATTCAGAGCTTGCCACGCTCTTCTTTACACCGACTCTGACAGAGATGGATTCATCGATGAGAAGGATTTCGAACAGCTCATCGATTATGCAGAGAAACGCCAATACACCGTGATTTAAATTTTTAGGATTTCTGTCTTTGCTTTATCTTTTAAGCTGAAATTAAGTTTGCAATTATTTCTTTTTTTGTTTGAACTCATTGTATATGACCAGAAGGAACTTTTTTTTTTTTTTTTTTGAATAAAGGACATTATTCTTACCAGTAAATTGCCACACTTTTGTACAAAGAAGCAAGAAGAGATGAGCTCTAGAAAGGCCGAACCTAAGGGAGAAAACTTTCAATAG

mRNA sequence

ATGCCTCGCAAAACCAAAACGTACCACCCGAGGCCCAGGATATCAAGGGAGCAATTGAGGCTAATCTTCAGAAACCACGACGACAACGGCGACGGCCGCCTCAGCTCCGCCGAGCTCACCCAAGCCTTCCACTATATCGGCGCCCTTTTGCCATCATTCAGAGCTTGCCACGCTCTTCTTTACACCGACTCTGACAGAGATGGATTCATCGATGAGAAGGATTTCGAACAGCTCATCGATTATGCAGAGAAACGCCAATACACCGACATTATTCTTACCAGTAAATTGCCACACTTTTGTACAAAGAAGCAAGAAGAGATGAGCTCTAGAAAGGCCGAACCTAAGGGAGAAAACTTTCAATAG

Coding sequence (CDS)

ATGCCTCGCAAAACCAAAACGTACCACCCGAGGCCCAGGATATCAAGGGAGCAATTGAGGCTAATCTTCAGAAACCACGACGACAACGGCGACGGCCGCCTCAGCTCCGCCGAGCTCACCCAAGCCTTCCACTATATCGGCGCCCTTTTGCCATCATTCAGAGCTTGCCACGCTCTTCTTTACACCGACTCTGACAGAGATGGATTCATCGATGAGAAGGATTTCGAACAGCTCATCGATTATGCAGAGAAACGCCAATACACCGACATTATTCTTACCAGTAAATTGCCACACTTTTGTACAAAGAAGCAAGAAGAGATGAGCTCTAGAAAGGCCGAACCTAAGGGAGAAAACTTTCAATAG
Homology
[Close]
BLAST of CmoCh04G009910 vs. Swiss-Prot
Match: POLC4_BETPN (Polcalcin Bet v 4 OS=Betula pendula GN=BETV4 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 9.0e-06
Identity = 26/67 (38.81%), Postives = 41/67 (61.19%), Query Frame = 1 

Query: 9  HPRPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSDRDG 68
          HP+ +  RE+   IF+  D NGDG++S+AEL +A   +G++ P     H +   D+D DG
Sbjct: 5  HPQDKAERER---IFKRFDANGDGKISAAELGEALKTLGSITPD-EVKHMMAEIDTDGDG 64

Query: 69 FIDEKDF 76
          FI  ++F
Sbjct: 65 FISFQEF 67

[Close]
BLAST of CmoCh04G009910 vs. TrEMBL
Match: A0A0A0KZR6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639730 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 2.6e-28
Identity = 63/90 (70.00%), Postives = 76/90 (84.44%), Query Frame = 1 

Query: 1  MPRKTKTYHPRPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALL 60
          M RK+K Y+PR ++SREQL LIFRNHD + DGRLS+ ELT+AF YIGA LP++RA HALL
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 YTDSDRDGFIDEKDFEQLIDYAEKRQYTDI 91
          + DSDRDGFI EKDFE L++YAE+RQYT I
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]
BLAST of CmoCh04G009910 vs. TrEMBL
Match: A5AWP8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023174 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 8.2e-14
Identity = 40/83 (48.19%), Postives = 57/83 (68.67%), Query Frame = 1 

Query: 6  KTYHPRPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSD 65
          KT   +  +  +QL+ IF+ HD NGDG+L+ AEL QAF ++GAL+P +RA  AL + D+D
Sbjct: 7  KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 66 RDGFIDEKDFEQLIDYAEKRQYT 89
           DG I EK+ + L++YA K  YT
Sbjct: 67 GDGCISEKEMKDLVEYAAKFGYT 89

[Close]
BLAST of CmoCh04G009910 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-12
Identity = 32/71 (45.07%), Postives = 54/71 (76.06%), Query Frame = 1 

Query: 14  ISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSDRDGFIDEK 73
           +SRE+++ IF+ HD+N DG L+  ELT+AF ++G++ P ++AC+ ++Y D++ DG I E 
Sbjct: 96  LSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEA 155

Query: 74  DFEQLIDYAEK 85
           + ++LIDYA K
Sbjct: 156 ELDKLIDYASK 166

[Close]
BLAST of CmoCh04G009910 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 5.9e-12
Identity = 34/77 (44.16%), Postives = 57/77 (74.03%), Query Frame = 1 

Query: 11 RPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSDRDGFI 70
          R ++SRE ++ IFR HD +GDGRLS  E+ +AF   G++LP ++A + + + D + DGFI
Sbjct: 7  RLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFI 66

Query: 71 DEKDFEQLIDYAEKRQY 88
          +E++ ++L+DYA+K Q+
Sbjct: 67 EEEELDKLVDYAQKFQH 83

[Close]
BLAST of CmoCh04G009910 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.3e-11
Identity = 34/75 (45.33%), Postives = 51/75 (68.00%), Query Frame = 1 

Query: 13 RISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSDRDGFIDE 72
          ++ +E+++ +FR HD NGDGRLS  EL++AF   GA  P +RA   L   D D DGFI E
Sbjct: 14 KLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISE 73

Query: 73 KDFEQLIDYAEKRQY 88
          ++ ++++DYA K +Y
Sbjct: 74 QELDKVVDYAIKCKY 88

[Close]
BLAST of CmoCh04G009910 vs. TAIR10
Match: AT2G43290.1 (AT2G43290.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 48.1 bits (113), Expect = 4.3e-06
Identity = 24/89 (26.97%), Postives = 42/89 (47.19%), Query Frame = 1 

Query: 1   MPRKTKTYHPRPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALL 60
           +P  + +  P  RI   +L+ +F+  D NGDGR++  EL  +   +G  +P       + 
Sbjct: 48  LPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIH 107

Query: 61  YTDSDRDGFIDEKDFEQLIDYAEKRQYTD 90
             D++ DG +D  +FE L        + D
Sbjct: 108 KIDANGDGCVDIDEFESLYSSIVDEHHND 136

[Close]
BLAST of CmoCh04G009910 vs. TAIR10
Match: AT1G76650.1 (AT1G76650.1 calmodulin-like 38)

HSP 1 Score: 47.0 bits (110), Expect = 9.6e-06
Identity = 24/69 (34.78%), Postives = 39/69 (56.52%), Query Frame = 1 

Query: 18  QLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSDRDGFIDEKDFEQ 77
           +L  +F   D N DGR+S  EL ++F  +G  L    A  A+  +D+D DG +D ++F Q
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 78  LIDYAEKRQ 87
           LI   ++ +
Sbjct: 103 LIKVDDEEE 111

[Close]
BLAST of CmoCh04G009910 vs. NCBI nr
Match: gi|700200027|gb|KGN55185.1| (hypothetical protein Csa_4G639730 [Cucumis sativus])

HSP 1 Score: 132.9 bits (333), Expect = 3.8e-28
Identity = 63/90 (70.00%), Postives = 76/90 (84.44%), Query Frame = 1 

Query: 1  MPRKTKTYHPRPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALL 60
          M RK+K Y+PR ++SREQL LIFRNHD + DGRLS+ ELT+AF YIGA LP++RA HALL
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 YTDSDRDGFIDEKDFEQLIDYAEKRQYTDI 91
          + DSDRDGFI EKDFE L++YAE+RQYT I
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]
BLAST of CmoCh04G009910 vs. NCBI nr
Match: gi|147866710|emb|CAN78405.1| (hypothetical protein VITISV_023174 [Vitis vinifera])

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-13
Identity = 40/83 (48.19%), Postives = 57/83 (68.67%), Query Frame = 1 

Query: 6  KTYHPRPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSD 65
          KT   +  +  +QL+ IF+ HD NGDG+L+ AEL QAF ++GAL+P +RA  AL + D+D
Sbjct: 7  KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 66 RDGFIDEKDFEQLIDYAEKRQYT 89
           DG I EK+ + L++YA K  YT
Sbjct: 67 GDGCISEKEMKDLVEYAAKFGYT 89

[Close]
BLAST of CmoCh04G009910 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 80.1 bits (196), Expect = 2.9e-12
Identity = 32/71 (45.07%), Postives = 54/71 (76.06%), Query Frame = 1 

Query: 14  ISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSDRDGFIDEK 73
           +SRE+++ IF+ HD+N DG L+  ELT+AF ++G++ P ++AC+ ++Y D++ DG I E 
Sbjct: 96  LSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEA 155

Query: 74  DFEQLIDYAEK 85
           + ++LIDYA K
Sbjct: 156 ELDKLIDYASK 166

[Close]
BLAST of CmoCh04G009910 vs. NCBI nr
Match: gi|659089539|ref|XP_008445564.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo])

HSP 1 Score: 78.6 bits (192), Expect = 8.4e-12
Identity = 37/71 (52.11%), Postives = 50/71 (70.42%), Query Frame = 1 

Query: 14  ISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSDRDGFIDEK 73
           +SREQ++ IF+ HD N DG LS  ELT+AF  +G+++P  +A HA+ Y D D DG I E 
Sbjct: 92  LSREQIKEIFKYHDSNKDGFLSIRELTKAFGSLGSIVPFAKARHAMAYADVDEDGLISEA 151

Query: 74  DFEQLIDYAEK 85
           +  +LIDYAEK
Sbjct: 152 ELHKLIDYAEK 162

[Close]
BLAST of CmoCh04G009910 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 78.6 bits (192), Expect = 8.4e-12
Identity = 34/77 (44.16%), Postives = 57/77 (74.03%), Query Frame = 1 

Query: 11  RPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALLYTDSDRDGFI 70
           R ++SRE ++ IFR HD +GDGRLS  E+ +AF   G++LP ++A + + + D + DGFI
Sbjct: 88  RLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFI 147

Query: 71  DEKDFEQLIDYAEKRQY 88
           +E++ ++L+DYA+K Q+
Sbjct: 148 EEEELDKLVDYAQKFQH 164

The following BLAST results are available for this feature:
BLAST of CmoCh04G009910 vs. Swiss-Prot
Match NameE-valueIdentityDescription
POLC4_BETPN9.0e-0638.81Polcalcin Bet v 4 OS=Betula pendula GN=BETV4 PE=1 SV=1[more]
BLAST of CmoCh04G009910 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KZR6_CUCSA2.6e-2870.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639730 PE=4 SV=1[more]
A5AWP8_VITVI8.2e-1448.19Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023174 PE=4 SV=1[more]
A0A0A0KMQ1_CUCSA2.0e-1245.07Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1[more]
A0A0A0LRC1_CUCSA5.9e-1244.16Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1[more]
A0A0A0LKW4_CUCSA1.3e-1145.33Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1[more]
BLAST of CmoCh04G009910 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G43290.14.3e-0626.97 Calcium-binding EF-hand family protein[more]
AT1G76650.19.6e-0634.78 calmodulin-like 38[more]
BLAST of CmoCh04G009910 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700200027|gb|KGN55185.1|3.8e-2870.00hypothetical protein Csa_4G639730 [Cucumis sativus][more]
gi|147866710|emb|CAN78405.1|1.2e-1348.19hypothetical protein VITISV_023174 [Vitis vinifera][more]
gi|700194510|gb|KGN49687.1|2.9e-1245.07hypothetical protein Csa_5G067410 [Cucumis sativus][more]
gi|659089539|ref|XP_008445564.1|8.4e-1252.11PREDICTED: probable calcium-binding protein CML11 [Cucumis melo][more]
gi|449470198|ref|XP_004152805.1|8.4e-1244.16PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G009910.1CmoCh04G009910.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 19..44
score: 4.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 14..49
score: 12.728coord: 63..85
score: 7
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 10..87
score: 1.6
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 10..90
score: 2.8
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 27..39
score: -coord: 63..75
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 15..82
score: 4.0
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 15..82
score: 4.0

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh04G009910Cucurbita moschata (Rifu)cmocmoB285
CmoCh04G009910Cucumber (Gy14) v1cgycmoB0118
CmoCh04G009910Cucurbita maxima (Rimu)cmacmoB337
CmoCh04G009910Cucurbita maxima (Rimu)cmacmoB728
CmoCh04G009910Wild cucumber (PI 183967)cmocpiB703
CmoCh04G009910Cucumber (Chinese Long) v2cmocuB693
CmoCh04G009910Melon (DHL92) v3.5.1cmomeB680
CmoCh04G009910Watermelon (Charleston Gray)cmowcgB615
CmoCh04G009910Cucurbita pepo (Zucchini)cmocpeB667
CmoCh04G009910Cucurbita pepo (Zucchini)cmocpeB673
CmoCh04G009910Bottle gourd (USVL1VR-Ls)cmolsiB678
CmoCh04G009910Bottle gourd (USVL1VR-Ls)cmolsiB684
CmoCh04G009910Cucumber (Gy14) v2cgybcmoB393
CmoCh04G009910Melon (DHL92) v3.6.1cmomedB775
CmoCh04G009910Silver-seed gourdcarcmoB0923
CmoCh04G009910Cucumber (Chinese Long) v3cmocucB0822
CmoCh04G009910Watermelon (97103) v2cmowmbB689