CmoCh03G014890 (gene) Cucurbita moschata (Rifu)

NameCmoCh03G014890
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionPhenylalanine-tRNA ligase, alpha subunit
LocationCmo_Chr03 : 10657403 .. 10662143 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAACTCCACCAAAGAGATAACCATCCACTTGGCATTCTGAAGAATGAGATATACAACTATTTTGATACAAATTACTCAAATAAGTTCACTAAGTTTGATGACCTCAGTCCAATCGTTTCTGTGAAGGAGGTAAGCCTTTTAAACTCATCAATTAGTTTTGAGGCTCAAATGAAGCTCATTCTATGACTATCTTTATGCCTGCGGAAATTTTAGTTTTCAGAAATGGATTAGTCCCGTAGCCAATTCGTTGAAAAATGGGTTTTACGCTGCTATAGAGCCTCTGAATTTGATATACCGCATTCTGATTAATATATCAGAGTGATTGAAGAATCTTTGATCTAGAAGGAACTAATGACAGAAAACATATGAAGGATAAAGAAATTGTGGCGTCCAGTGGTCCAAATAAGCTCATATGAAAGAGCATCTTCTGATCTCATGAAGTTCACTAACTTAACACCCTTGTATTCATTTGACTCACTAATAACCTTTTGATTTACATCTTTATTTTCTATGGAGGCCAATGTGGTCAATTTGGAATTATCAATTTTCTCTCTAGAGTAATAATAACATATTTGAAACCAATTTCAAACTTGATGGATTAATTAAAAGTTAAATGCAAATCTCGAAGACCAAGTGTATCACTTCTGAAATAACTAAACAGAAACTACATTCAAACTTTAAGGACCAAAAAGTAATTCAAATCTTGGGACCAAACACCTTAAGGACCAAATAGAACTAAATTTAATGTTCACGGACCAAAAGTTTTGGCTTTGAAATCTCAGGAACAATTAGAAGCTAAGTTCAATACTAAGGGACCGAAAGTGTATTTTTTCAAAAAAAAAAGAAACTTTCATTCTTTTCGTGATTTTTATTGAAGTACTGAACTTATTTTGGAAGATGGCGGGATCGGTTTGGTATTTGTCAACTTTCCTGTTGACTGGTCAAATTTTTTCCCTAAATTCCAATAGCTTATCGTGTTGTTTATTTATTAATTAATCATTTTATAATAACAATTATTTTTAAACATACAAAGCCTCTTGTATCTGAATCCTATAATTATGTCTGCAGAATTTTGATGATGTGTTGGTTCCAGAAGATCATGTAAGCCGAAGTTATAATGATACATACTACATTGACTCGCAAACTGTTCTGAGATGTCATACAAGTGCTCACCAAGCGGAGCTGTTGAGAAGAGGCTATACTCAGTTTCTTGTGACAGGCGATGTTTATCGTCGGGATTCCATTGATTCAACTCATTACCCTGTATTTCATCAGGTAATCTAAATAAGTTTATTAATCATGTCATTTGCTAAATTTACCATAGTTTTCACTTAATGAATTTCTTTGCCTACTTCTCAGATGGAAGGTGTCCGTGTTTTTTCTCTGAATGATTGGGAGGCATCTGGTACAGATGGAACATCTTTTGCAGCTGACGACTTGAAGAAATGTTTGGAAGGTTTGGCAAGGCATTTATTTGGTAAGAGTTGTGTATGAATTAACCCATGATTCTTCCCTTTCTATCTTAGTTATGGTTGGGGATTCTATTGAAAATTCATATGGGAATACAAACGATTTTTGGGTAGAGACAAAACAATAGCACTTGCAGAATAATCGGTTAAAAGACACAAACTGTAGAAGTAATAAGTATAATGTATTGGATAAGTATTAATTATTTTTATTTTGCATATGATTATTATAAAAACTTATTCCAAATGTAGTTACATTTTTAAGATAAAAACGAAAAATAGATTCCCAACTTACGTAATTAGGCTTTGATTTTTTAAAGTAATTTCTGTAATTTTGCAGTGGTTGTACACATTATGCAGCTATGTTATTATAAACTGATGTATTAAAATTTCCAGGTGCTGTGGAAATGCGCTGGGTTGATACATATTTCCCATTTACAAATCCATCATTTGAACTTGAGATTTTCTTTCAGGTACGTCAGATGATCGTTTAGTCAATTTGCTCTTCAGAACTCAATAATTTGATTATGGTACACTGCATTACTTCTTTTATGAATGACTTTCATTGCCGCCGATATATATATATATATATATATATATATATATATAAGTTTAAATATGTCAAGTTTAATTCCATTTACACAATGGCATTTCTGTTTTATCATATATGAACTTTAAATAGAACCTAGATGATAACCTTACAACTTTTTGACTTTGATAAGTTAGACGAGGGAACCAAGAAGTGAAAACGTGCTTGGGAGGGGACAGATGTTTTTCTGTATTTTTGGGTGTGTGTGGGTGGGGGGTTATTTTGGGATAAAGAAACTTTCATTATCCAAAAACAATACAAAGATGATAAGTTATCAGCCTGACATAGGCAAGAGGGTAACAAAAGGTTCCTCTAATTAGGATTGGCCACAACCATTTCTCAGCTATTATTTCCGCCCATGAATCCTATAAAAGAATGAATGGATGATATTTGAATTATGAGATTTCCTCGTCAAATTATTTACATTGCATTTGCTGTTAGTTCCTTCTTTGGATCTGTAGTTGTTTTCTCTCTTTCTGTTCAACAAAAAATATAATAAAAAGTAATCCTAAAATTTGAGGGCATTTGATGTCCTACCCACGCAGGAGAAATGGCTGGAAGTATTGGGATGCGGGGTGATGGAGCAAGAAATATTGAAAAGAAGTGATAAAGAAAATAATGTTGCATGGGCTTTTGGTCTCGGGTTGGAAAGGCTAGCTATGGTTCTTTTTGATATACCTGATATCCGATTATTTTGGTCAGCTGACGAGCGATTTACCACGCAGGTACCTAATTGCACCACTTAAACTATACTCTTTCATTTTTTCCACTCTGTTTTCTCTTTGAAAATAAATTATCCAATCATCACGTTGGGCTTTTTATTTGCAGTTCTCAAAGGGCAAGCTAGGTGTCAAGTTTAAATCGTTTTCTAAGGTACTTTTACCAAACATTTTAGGCATGAAGGGATAACAATCTTCCGTTTCCTGAGAGAGCATATAATTCTATTTCTTTTAGTAACATCGAACTAGTTTCAATATGCTTTCTAACAAATTACATCTTCTAAATTATTTTAAAAAACTTTAGTAATTCAATCTGCTGCAGTTTCCACCTTGCTACAAGGACATCAGTTTTTGGATCAATGATTCCTTCACCGAGAATAATTTATGTGAAGTTGTTAGAGGAGTTGCCGGCGACCTTGTTGAAGAGGTATTTTATTAACTGTTTTCAGCGCATATATAATTCTTAAGATAATTTTAATGAAATGCTTAAGGTAGGGGCATGAGAACCTTTTTCTTCTCGAAATACATGCTTGAGAAAATCTGCTATCTGAAGTTCAATTTTTTAAAAGATAAAGTTGGTTGTGTAAATCTGAAAACGTTCTTTCAATCATGGATACTCTTTCAATTAACCTGGTCTGTTAGTTTCCGGAAAGTTGCACCTCGTTTAATGAAGTCATTTTAATTTTTTACAAATGGATGCTGGCAGGTACAACTGATAGACAGTTTTACCAACAAGAAGAAAGGGATGACGAGTCACTGTTACAGGATTGCTTACCGATCTATGGAACGGTCACTCACAGATGAAGAAATCAATGATTTGCAGGTATTTATCAACTTTAAGTTATATCCCTATTATATATTAACTTGTTTGAAAAAGAAGTTTGGATACAGCCTTCATATATTTTGTGGTTGCAAATGCAGTGGAACGTGAGAGAACAGGTTCAGAGCAAGTTGAACGTTGTCTTGCGATGAACATTGGAAGTGCAACAATATCAGTCCAAGCCATGTAATGTTATTTTTTACCTTCTCTACAGATTGAATTTTACTCTGTACACCATTTGAATTTTGCCTACAAAATTCTTCTAACTGGGTGATAGGAAGTGTAGAGTATATAACGTAACCAGTTGAAACCCTGAAAATAATCATCAAGAATTCAAGTGGAGATGTGCTGCAACTAATTTTTTAACATATACTATGAGTTTCTTTCCCCTACCTCATGTTTTAACATGACTTTGCTGATTTTTGGACCTGTTGAACTTATGCTATCAAGAAACTCCATTTACCATTGGACCACTTTTGCCTAGATTAGAAAGGGAGACCTCACTTTGTCTGAAATATATGACAAAGGAATAAGAATGTGTGCTTTGAAGTAAGTTGTGCCTATTTTCTTGACATCCGAACAAATAGAAGCTGAGGGAAGGTACTTTTCCTGAATAGTTGAGCCAATTTTGATATTTCTTTAGAAACTGACGAAGCCTGCAGGTTAAAATGCTGTACTTAGAAAATGTGCAAACCTTTAACAACACATAAAATAACCAAACTTGAACTAGAGCAAGATAATCTAGTGGTCAAAATGATGTCACGATAGACATCAACCCAAAGAAATAAAAGAACGAATATCATTTTCCAACACCCCTTCCAACTACCGATTTACCTTGCAATTCTAGTAAATATGATAAAGACCTCTTATGATATGAGATTCCCAAATCAAAACTGCATGAAAAGCAATTCACACACACTTTAAATAACAAAATATTTAATTTTATAGCACAATCAATTAAGAAGGGTTAAGTATTTGCCTGATTAAGTCATATAATGTCTTTAAAAGTTCGATCTCCCGACTCTAGGAGCTCAAAAGTACAGAGATATTGGATTTCACAGTTCCATTCACATGCATTTTAAATTTTATTCTTATATAAACTCTGTAGGCAAATGCCTCTTTTTAATTTTAAGGAGTATTTAGT

mRNA sequence

ATGCAACTCCACCAAAGAGATAACCATCCACTTGGCATTCTGAAGAATGAGATATACAACTATTTTGATACAAATTACTCAAATAAGTTCACTAAGTTTGATGACCTCAGTCCAATCGTTTCTGTGAAGGAGAATTTTGATGATGTGTTGGTTCCAGAAGATCATGTAAGCCGAAGTTATAATGATACATACTACATTGACTCGCAAACTGTTCTGAGATGTCATACAAGTGCTCACCAAGCGGAGCTGTTGAGAAGAGGCTATACTCAGTTTCTTGTGACAGGCGATGTTTATCGTCGGGATTCCATTGATTCAACTCATTACCCTGTATTTCATCAGATGGAAGGTGTCCGTGTTTTTTCTCTGAATGATTGGGAGGCATCTGGTACAGATGGAACATCTTTTGCAGCTGACGACTTGAAGAAATGTTTGGAAGGTTTGGCAAGGCATTTATTTGGTGCTGTGGAAATGCGCTGGGTTGATACATATTTCCCATTTACAAATCCATCATTTGAACTTGAGATTTTCTTTCAGGAGAAATGGCTGGAAGTATTGGGATGCGGGGTGATGGAGCAAGAAATATTGAAAAGAAGTGATAAAGAAAATAATGTTGCATGGGCTTTTGGTCTCGGGTTGGAAAGGCTAGCTATGGTTCTTTTTGATATACCTGATATCCGATTATTTTGGTCAGCTGACGAGCGATTTACCACGCAGTTCTCAAAGGGCAAGCTAGGTGTCAAGTTTAAATCGTTTTCTAAGTTTCCACCTTGCTACAAGGACATCAGTTTTTGGATCAATGATTCCTTCACCGAGAATAATTTATGTGAAGTTGTTAGAGGAGTTGCCGGCGACCTTGTTGAAGAGGTACAACTGATAGACAGTTTTACCAACAAGAAGAAAGGGATGACGAGTCACTGTTACAGGATTGCTTACCGATCTATGGAACGGTCACTCACAGATGAAGAAATCAATGATTTGCAGTGGAACGTGAGAGAACAGGTTCAGAGCAAGTTGAACGTTGTCTTGCGATGAACATTGGAAGTGCAACAATATCAGTCCAAGCCATGTAATGTTATTTTTTACCTTCTCTACAGATTGAATTTTACTCTGTACACCATTTGAATTTTGCCTACAAAATTCTTCTAACTGGGTGATAGGAAGTGTAGAGTATATAACGTAACCAGTTGAAACCCTGAAAATAATCATCAAGAATTCAAGTGGAGATGTGCTGCAACTAATTTTTTAACATATACTATGAGTTTCTTTCCCCTACCTCATGTTTTAACATGACTTTGCTGATTTTTGGACCTGTTGAACTTATGCTATCAAGAAACTCCATTTACCATTGGACCACTTTTGCCTAGATTAGAAAGGGAGACCTCACTTTGTCTGAAATATATGACAAAGGAATAAGAATGTGTGCTTTGAAGTAAGTTGTGCCTATTTTCTTGACATCCGAACAAATAGAAGCTGAGGGAAGGTACTTTTCCTGAATAGTTGAGCCAATTTTGATATTTCTTTAGAAACTGACGAAGCCTGCAGGTTAAAATGCTGTACTTAGAAAATGTGCAAACCTTTAACAACACATAAAATAACCAAACTTGAACTAGAGCAAGATAATCTAGTGGTCAAAATGATGTCACGATAGACATCAACCCAAAGAAATAAAAGAACGAATATCATTTTCCAACACCCCTTCCAACTACCGATTTACCTTGCAATTCTAGTAAATATGATAAAGACCTCTTATGATATGAGATTCCCAAATCAAAACTGCATGAAAAGCAATTCACACACACTTTAAATAACAAAATATTTAATTTTATAGCACAATCAATTAAGAAGGGTTAAGTATTTGCCTGATTAAGTCATATAATGTCTTTAAAAGTTCGATCTCCCGACTCTAGGAGCTCAAAAGTACAGAGATATTGGATTTCACAGTTCCATTCACATGCATTTTAAATTTTATTCTTATATAAACTCTGTAGGCAAATGCCTCTTTTTAATTTTAAGGAGTATTTAGT

Coding sequence (CDS)

ATGCAACTCCACCAAAGAGATAACCATCCACTTGGCATTCTGAAGAATGAGATATACAACTATTTTGATACAAATTACTCAAATAAGTTCACTAAGTTTGATGACCTCAGTCCAATCGTTTCTGTGAAGGAGAATTTTGATGATGTGTTGGTTCCAGAAGATCATGTAAGCCGAAGTTATAATGATACATACTACATTGACTCGCAAACTGTTCTGAGATGTCATACAAGTGCTCACCAAGCGGAGCTGTTGAGAAGAGGCTATACTCAGTTTCTTGTGACAGGCGATGTTTATCGTCGGGATTCCATTGATTCAACTCATTACCCTGTATTTCATCAGATGGAAGGTGTCCGTGTTTTTTCTCTGAATGATTGGGAGGCATCTGGTACAGATGGAACATCTTTTGCAGCTGACGACTTGAAGAAATGTTTGGAAGGTTTGGCAAGGCATTTATTTGGTGCTGTGGAAATGCGCTGGGTTGATACATATTTCCCATTTACAAATCCATCATTTGAACTTGAGATTTTCTTTCAGGAGAAATGGCTGGAAGTATTGGGATGCGGGGTGATGGAGCAAGAAATATTGAAAAGAAGTGATAAAGAAAATAATGTTGCATGGGCTTTTGGTCTCGGGTTGGAAAGGCTAGCTATGGTTCTTTTTGATATACCTGATATCCGATTATTTTGGTCAGCTGACGAGCGATTTACCACGCAGTTCTCAAAGGGCAAGCTAGGTGTCAAGTTTAAATCGTTTTCTAAGTTTCCACCTTGCTACAAGGACATCAGTTTTTGGATCAATGATTCCTTCACCGAGAATAATTTATGTGAAGTTGTTAGAGGAGTTGCCGGCGACCTTGTTGAAGAGGTACAACTGATAGACAGTTTTACCAACAAGAAGAAAGGGATGACGAGTCACTGTTACAGGATTGCTTACCGATCTATGGAACGGTCACTCACAGATGAAGAAATCAATGATTTGCAGTGGAACGTGAGAGAACAGGTTCAGAGCAAGTTGAACGTTGTCTTGCGATGA
BLAST of CmoCh03G014890 vs. Swiss-Prot
Match: SYFM_ARATH (Phenylalanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=At3g58140 PE=1 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 3.0e-171
Identity = 281/343 (81.92%), Postives = 312/343 (90.96%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           MQLH+RD HP+GILKN IY+YFD+NYSNKF KF+DLSPIV+ K+NFDDVLVP DHVSRS 
Sbjct: 87  MQLHRRDKHPIGILKNAIYDYFDSNYSNKFEKFEDLSPIVTTKQNFDDVLVPADHVSRSL 146

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYY+DSQTVLRCHTSAHQAELLR+G+++FLVTGDVYRRDSIDSTHYPVFHQMEG  VF
Sbjct: 147 NDTYYVDSQTVLRCHTSAHQAELLRKGHSRFLVTGDVYRRDSIDSTHYPVFHQMEGFCVF 206

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           S  DW  SG D T +AA+DLKKCLEGLARHLFG+VEMRWVDTYFPFTNPSFELEI+F+E 
Sbjct: 207 SPEDWNGSGKDSTLYAAEDLKKCLEGLARHLFGSVEMRWVDTYFPFTNPSFELEIYFKED 266

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGV EQ ILK+S  ENNVAWAFGLGLERLAMVLFDIPDIR FWS+DERFT+QF 
Sbjct: 267 WLEVLGCGVTEQVILKQSGLENNVAWAFGLGLERLAMVLFDIPDIRFFWSSDERFTSQFG 326

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
           KG+LGVKFK +SK+PPCYKDISFWI+D FTENN CEVVRG+AGDLVEEV+LID FTNKKK
Sbjct: 327 KGELGVKFKPYSKYPPCYKDISFWISDLFTENNFCEVVRGIAGDLVEEVKLIDQFTNKKK 386

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           G+TSHCYRI +RSMERSLTDEE+NDLQ  VR++VQ KLNV LR
Sbjct: 387 GLTSHCYRIVFRSMERSLTDEEVNDLQSKVRDEVQKKLNVELR 429

BLAST of CmoCh03G014890 vs. Swiss-Prot
Match: SYFM_DICDI (Phenylalanine--tRNA ligase, mitochondrial OS=Dictyostelium discoideum GN=mpheS PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.1e-100
Identity = 193/391 (49.36%), Postives = 246/391 (62.92%), Query Frame = 1

Query: 3   LHQRDNHPLGILKNEIYNYFDTNYSN---KFTKFDDLSPIVSVKENFDDVLVPEDHVSRS 62
           LH + +HPL I+K +I  +F    S+   KF  FD   P VSVKENFD++L P DHV RS
Sbjct: 64  LHCKRDHPLNIIKKKIQYHFQNKLSDEEHKFQFFDSFEPKVSVKENFDELLFPVDHVGRS 123

Query: 63  YNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVR- 122
            NDTYY     +LR HTSAHQ++LLR     FLVTGDVYRRD+ID+ HYPVFHQMEGV+ 
Sbjct: 124 PNDTYYFSKDQLLRTHTSAHQSQLLREQEKAFLVTGDVYRRDTIDAVHYPVFHQMEGVKV 183

Query: 123 -----------------VFSLNDWEASGTDGTSFAADDLKKCLEGLARHLFGA-VEMRWV 182
                            +    D              DLK+ LE + R + G  +++RW+
Sbjct: 184 FKDKVNLAIDGKPFDETIDYYEDNNYKQLQSVKDVEKDLKQSLESMIRSVIGQDLQVRWI 243

Query: 183 DTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLF 242
           D YFPFT+PSFE+EI+FQ +WLEVLGCGV+   I+      N+ AWAFG+GLERLAM+LF
Sbjct: 244 DAYFPFTSPSFEMEIYFQGQWLEVLGCGVVHPSIMNNCGLSNDRAWAFGIGLERLAMILF 303

Query: 243 DIPDIRLFWSADERFTTQF--------------------SKGKL------GVKFKSFSKF 302
           +IPDIRLFW+ D RF  QF                    S   L      GV+F+ FSK+
Sbjct: 304 NIPDIRLFWTEDNRFHNQFKGVDKSISTSSSSSSSSSSSSSSTLSDIDIKGVQFQQFSKY 363

Query: 303 PPCYKDISFWIND--SFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYR 344
           P C+KD+SFW+ D  +F EN   E VR   GDLVE V L+D+FTNKK   TSHCYRI YR
Sbjct: 364 PSCFKDVSFWLEDEENFHENKFYEFVRESCGDLVERVDLVDNFTNKKLNKTSHCYRIYYR 423

BLAST of CmoCh03G014890 vs. Swiss-Prot
Match: SYFM_MOUSE (Phenylalanine--tRNA ligase, mitochondrial OS=Mus musculus GN=Fars2 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.7e-95
Identity = 180/370 (48.65%), Postives = 235/370 (63.51%), Query Frame = 1

Query: 3   LHQRDNHPLGILKNEIYNYFDTNYSNK-----FTKFDDLSPIVSVKENFDDVLVPEDHVS 62
           LH +  HPL ++K  +  +F   Y  +     F+ +D L P+V+  +NFD +L+P DH S
Sbjct: 78  LHNQKFHPLWLIKERVKEHFYQQYMVRSRTPLFSVYDQLPPVVTTWQNFDSLLIPADHPS 137

Query: 63  RSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGV 122
           R   D YY++   +LR HTSAHQ +LL  G   FLV GDVYRRD ID  HYPVFHQ+EGV
Sbjct: 138 RKKGDNYYLNRAHMLRAHTSAHQWDLLHAGLNAFLVVGDVYRRDQIDCQHYPVFHQLEGV 197

Query: 123 RVFSLNDWEASGTDGTS-----------------------FAADDLKKCLEGLARHLFG- 182
           R+FS ++  A   DG S                           DLK+ L  L  HLFG 
Sbjct: 198 RLFSKHELFAGVKDGESLQLFEEGSRSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGD 257

Query: 183 AVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLE 242
            +E+RWVD YFPFT+PSFE+EI F+ +WLEVLGCGVMEQ+++  +  ++ + WAFGLGLE
Sbjct: 258 GLEVRWVDCYFPFTHPSFEMEINFRGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLE 317

Query: 243 RLAMVLFDIPDIRLFWSADERFTTQF--SKGKLGVKFKSFSKFPPCYKDISFWI-NDSFT 302
           RLAMVL+DIPDIRLFWS DERF  QF  S     VKF+  SK+P  + DISFW+ ++++T
Sbjct: 318 RLAMVLYDIPDIRLFWSEDERFLKQFLLSDINQSVKFQPLSKYPAVFNDISFWLPSENYT 377

Query: 303 ENNLCEVVRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNV 341
           EN+  ++VR V GDLVE+V LID F + K   TSHCYRI YR MER+L+  E+ ++   V
Sbjct: 378 ENDFYDIVRTVGGDLVEKVDLIDKFEHPKTHRTSHCYRITYRHMERTLSQREVGNVHQAV 437

BLAST of CmoCh03G014890 vs. Swiss-Prot
Match: SYFM_HUMAN (Phenylalanine--tRNA ligase, mitochondrial OS=Homo sapiens GN=FARS2 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.7e-94
Identity = 174/370 (47.03%), Postives = 233/370 (62.97%), Query Frame = 1

Query: 3   LHQRDNHPLGILKNEIYNYFDTNYSNKF-----TKFDDLSPIVSVKENFDDVLVPEDHVS 62
           LH + +HPL ++K  +  +F   Y  +F     + +D+LSP+V+  +NFD +L+P DH S
Sbjct: 78  LHNQQHHPLWLIKERVKEHFYKQYVGRFGTPLFSVYDNLSPVVTTWQNFDSLLIPADHPS 137

Query: 63  RSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGV 122
           R   D YY++   +LR HTSAHQ +LL  G   FLV GDVYRRD IDS HYP+FHQ+E V
Sbjct: 138 RKKGDNYYLNRTHMLRAHTSAHQWDLLHAGLDAFLVVGDVYRRDQIDSQHYPIFHQLEAV 197

Query: 123 RVFSLNDWEASGTDGTS-----------------------FAADDLKKCLEGLARHLFG- 182
           R+FS ++  A   DG S                           DLK+ L  L  HLFG 
Sbjct: 198 RLFSKHELFAGIKDGESLQLFEQSSRSAHKQETHTMEAVKLVEFDLKQTLTRLMAHLFGD 257

Query: 183 AVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLE 242
            +E+RWVD YFPFT+PSFE+EI F  +WLEVLGCGVMEQ+++  +  ++ + WAFGLGLE
Sbjct: 258 ELEIRWVDCYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLE 317

Query: 243 RLAMVLFDIPDIRLFWSADERFTTQFSKGKLG--VKFKSFSKFPPCYKDISFWI-NDSFT 302
           RLAM+L+DIPDIRLFW  DERF  QF    +   VKF+  SK+P    DISFW+ ++++ 
Sbjct: 318 RLAMILYDIPDIRLFWCEDERFLKQFCVSNINQKVKFQPLSKYPAVINDISFWLPSENYA 377

Query: 303 ENNLCEVVRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNV 341
           EN+  ++VR + GDLVE+V LID F + K   TSHCYRI YR MER+L+  E+  +   +
Sbjct: 378 ENDFYDLVRTIGGDLVEKVDLIDKFVHPKTHKTSHCYRITYRHMERTLSQREVRHIHQAL 437

BLAST of CmoCh03G014890 vs. Swiss-Prot
Match: SYFM_RAT (Phenylalanine--tRNA ligase, mitochondrial OS=Rattus norvegicus GN=Fars2 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.3e-93
Identity = 178/391 (45.52%), Postives = 235/391 (60.10%), Query Frame = 1

Query: 3   LHQRDNHPLGILKNEIYNYFDTNYSNK-----FTKFDDLSPIVSVKENFDDVLVPEDHVS 62
           LH +  HPL ++K  +  +F   Y  +     F+ +D LSP+V+  +NFD +L+P DH S
Sbjct: 78  LHNQKFHPLWLIKERVKEHFYQQYMGRSRTPLFSVYDQLSPVVTTWQNFDSLLIPADHPS 137

Query: 63  RSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGV 122
           R   D YY++   +LR HTSAHQ +LL  G   FLV GDVYRRD IDS HYPVFHQ+EGV
Sbjct: 138 RKKGDNYYLNRGHMLRAHTSAHQWDLLHAGLNAFLVVGDVYRRDQIDSQHYPVFHQLEGV 197

Query: 123 RVF-----------------------SLNDWEASGTDGTSFAADDLKKCLEGLARHLFG- 182
           R+F                       S +  E    +       DLK+ L  L  HLFG 
Sbjct: 198 RLFSKHELFAGVKDGESLQLFEESSRSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGD 257

Query: 183 AVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGLE 242
            +E+RWVD YFPFT+PSFE+EI F+ +WLEVLGCGVMEQ+++  +  ++ + WAFGLGLE
Sbjct: 258 GLEVRWVDCYFPFTHPSFEMEINFRGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLE 317

Query: 243 RLAMVLFDIPDIRLFWSADERFTTQFS-----------------------KGKLGVKFKS 302
           RLAMVL+DIPDIRLFWS DERF  QF                        + K+G +   
Sbjct: 318 RLAMVLYDIPDIRLFWSEDERFLKQFRLSDINQSVKFQRWFFQEERATGIQRKMGRQLCP 377

Query: 303 FSKFPPCYKDISFWI-NDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRI 341
           FSK+P  + DISFW+ ++++TEN+  ++VR V GDLVE+V LID F + K   TSHCYRI
Sbjct: 378 FSKYPAVFNDISFWLPSENYTENDFYDIVRTVGGDLVEKVDLIDKFEHPKTHRTSHCYRI 437

BLAST of CmoCh03G014890 vs. TrEMBL
Match: E5GCL5_CUCME (Phenylalanyl-tRNA synthetase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 5.0e-189
Identity = 321/343 (93.59%), Postives = 332/343 (96.79%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           MQLH+RDNHPLGILKNEIYNYFDTNYSNKF KFDDLSPIVSVKENFDDVLVPEDHVSRSY
Sbjct: 89  MQLHRRDNHPLGILKNEIYNYFDTNYSNKFIKFDDLSPIVSVKENFDDVLVPEDHVSRSY 148

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQME VRVF
Sbjct: 149 NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEAVRVF 208

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           SLND E SGTDGTS+AADDLKKCLEGLARHLFGAVEMRWVD YFPFT+PSFELEIFFQEK
Sbjct: 209 SLNDLEVSGTDGTSYAADDLKKCLEGLARHLFGAVEMRWVDAYFPFTDPSFELEIFFQEK 268

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGVMEQ+ILK+  KEN+VAWAFGLGLERLAMVLF IPD+RLFWSADERFT+QFS
Sbjct: 269 WLEVLGCGVMEQKILKKCGKENHVAWAFGLGLERLAMVLFGIPDVRLFWSADERFTSQFS 328

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
           KGKLGVKFK FSKFPPCYKDISFWIN+SFTENNLCEVVRG+AGDLVEEVQLIDSFTN+KK
Sbjct: 329 KGKLGVKFKPFSKFPPCYKDISFWINESFTENNLCEVVRGIAGDLVEEVQLIDSFTNEKK 388

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           GMTSHCYRIAYRSMERSLTDEEINDLQ NVREQVQSKL VVLR
Sbjct: 389 GMTSHCYRIAYRSMERSLTDEEINDLQRNVREQVQSKLKVVLR 431

BLAST of CmoCh03G014890 vs. TrEMBL
Match: A0A0A0KJ29_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G526470 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 3.2e-188
Identity = 319/343 (93.00%), Postives = 332/343 (96.79%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           MQLH+RDNHPLGILKNEIYNYFDTNYSNKF KFDDLSPIVSVKENFDDVLVPEDHVSRSY
Sbjct: 89  MQLHRRDNHPLGILKNEIYNYFDTNYSNKFIKFDDLSPIVSVKENFDDVLVPEDHVSRSY 148

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQME VRVF
Sbjct: 149 NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEAVRVF 208

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           SL+D E SGTDGTS+AADDLKKCLEGLARHLFGAVEMRWVD YFPFT+PSFELEIFFQEK
Sbjct: 209 SLDDLEVSGTDGTSYAADDLKKCLEGLARHLFGAVEMRWVDAYFPFTDPSFELEIFFQEK 268

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGVMEQ+ILK+  KEN+V+WAFGLGLERLAMVLF IPD+RLFWSADERFT+QFS
Sbjct: 269 WLEVLGCGVMEQKILKKCGKENHVSWAFGLGLERLAMVLFGIPDVRLFWSADERFTSQFS 328

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
           KGKLGVKFK FSKFPPCYKDISFWIN+SFTENNLCEVVRG+AGDLVEEVQLIDSFTN+KK
Sbjct: 329 KGKLGVKFKPFSKFPPCYKDISFWINESFTENNLCEVVRGIAGDLVEEVQLIDSFTNEKK 388

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           G TSHCYRIAYRSMERSLTDEEINDLQ NVREQVQSKLNVVLR
Sbjct: 389 GTTSHCYRIAYRSMERSLTDEEINDLQTNVREQVQSKLNVVLR 431

BLAST of CmoCh03G014890 vs. TrEMBL
Match: B9MWE6_POPTR (Phenylalanyl-tRNA synthetase class IIc family protein OS=Populus trichocarpa GN=POPTR_0013s10990g PE=4 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 1.6e-179
Identity = 303/343 (88.34%), Postives = 325/343 (94.75%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           +QLH+RD HPLGILKN IY+YFDTNYSNKF KFD+L PIVSVK+NFDDVLVP DHVSRSY
Sbjct: 85  LQLHRRDQHPLGILKNTIYDYFDTNYSNKFDKFDELCPIVSVKQNFDDVLVPADHVSRSY 144

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYY+DSQTVLRCHTSAHQAELLR+G+T FLVTGDVYRRDSIDSTHYPVFHQMEG RVF
Sbjct: 145 NDTYYVDSQTVLRCHTSAHQAELLRKGHTHFLVTGDVYRRDSIDSTHYPVFHQMEGFRVF 204

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           S ++WEASG DGTSFAA+DLKKCLEGLA HLFGAVEMRWVDTYFPFTNPSFELEI+FQEK
Sbjct: 205 SPDEWEASGVDGTSFAAEDLKKCLEGLATHLFGAVEMRWVDTYFPFTNPSFELEIYFQEK 264

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGV EQEILKR+ K NNVAWAFGLGLERLAMVLFDIPDIRLFWS DERFT+QFS
Sbjct: 265 WLEVLGCGVTEQEILKRNGKLNNVAWAFGLGLERLAMVLFDIPDIRLFWSNDERFTSQFS 324

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
           KG+LG+KFK FSK+PPCYKD+SFWIN+SFTENNLCEVVRG+AGDLVEEV LID+FTN KK
Sbjct: 325 KGQLGIKFKPFSKYPPCYKDMSFWINESFTENNLCEVVRGIAGDLVEEVSLIDNFTN-KK 384

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           GMTSHCYRIAYRSMERSLTDEEINDLQ +VREQVQSKLNVVLR
Sbjct: 385 GMTSHCYRIAYRSMERSLTDEEINDLQMSVREQVQSKLNVVLR 426

BLAST of CmoCh03G014890 vs. TrEMBL
Match: M5WGS0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006022mg PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 3.6e-179
Identity = 303/343 (88.34%), Postives = 322/343 (93.88%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           MQLH+RD HP+GILKN IY YFDTNYSN+F KFD+L PIVSVKENFDDVLVP DHVSRSY
Sbjct: 91  MQLHRRDQHPIGILKNAIYEYFDTNYSNQFDKFDNLCPIVSVKENFDDVLVPADHVSRSY 150

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYY+D+QTVLRCHTSAHQAELLRRG+T FLVTGDVYRRDSIDSTHYPVFHQMEGVRVF
Sbjct: 151 NDTYYVDAQTVLRCHTSAHQAELLRRGHTHFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 210

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           S +DWEASGTDGT++ A DLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK
Sbjct: 211 SPSDWEASGTDGTTYVAGDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 270

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGV EQEIL+RS K +NVAWAFGLGLERLAMVLFDIPDIRLFWS DERFT+QFS
Sbjct: 271 WLEVLGCGVTEQEILRRSGKTDNVAWAFGLGLERLAMVLFDIPDIRLFWSNDERFTSQFS 330

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
            G+LGVKFK FSK+PPCYKD+SFWIN++FTENNLCEVVR VAGDL EEVQLID+FTN KK
Sbjct: 331 SGQLGVKFKPFSKYPPCYKDVSFWINEAFTENNLCEVVREVAGDLAEEVQLIDNFTN-KK 390

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           GMTSHCYRIAYRSMERSLTDEEIN LQWNVRE VQSKLNVVLR
Sbjct: 391 GMTSHCYRIAYRSMERSLTDEEINKLQWNVRELVQSKLNVVLR 432

BLAST of CmoCh03G014890 vs. TrEMBL
Match: A0A151TD69_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_019602 PE=4 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 5.2e-178
Identity = 301/343 (87.76%), Postives = 319/343 (93.00%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           MQLH+RD HPLGILKN IY YFDTNYSN F KFDDL PIVSVK+NFDDVLVP+DHVSRSY
Sbjct: 88  MQLHRRDQHPLGILKNAIYEYFDTNYSNNFNKFDDLCPIVSVKQNFDDVLVPKDHVSRSY 147

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYYID QTVLRCHTSAHQAELLR GYT FLVTGDVYRRDSIDSTHYPVFHQMEG RVF
Sbjct: 148 NDTYYIDPQTVLRCHTSAHQAELLRSGYTHFLVTGDVYRRDSIDSTHYPVFHQMEGFRVF 207

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           +  +WEASG D T ++A DLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEI+F+EK
Sbjct: 208 APEEWEASGMDATLYSATDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIYFKEK 267

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGV EQEILK + K NNVAWAFGLGLERLAMVLFDIPDIRLFWS DERFT+QFS
Sbjct: 268 WLEVLGCGVTEQEILKSNGKTNNVAWAFGLGLERLAMVLFDIPDIRLFWSNDERFTSQFS 327

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
           KG+LGVKFK FSK+PPCYKD+SFWIN+SFTENNLCEVVRG+AGDLVEEVQLID+FTN KK
Sbjct: 328 KGQLGVKFKPFSKYPPCYKDMSFWINESFTENNLCEVVRGIAGDLVEEVQLIDNFTN-KK 387

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           G+TSHCYRIAYRSMERSLTDEEINDLQW VREQVQSKLNVVLR
Sbjct: 388 GITSHCYRIAYRSMERSLTDEEINDLQWKVREQVQSKLNVVLR 429

BLAST of CmoCh03G014890 vs. TAIR10
Match: AT3G58140.1 (AT3G58140.1 phenylalanyl-tRNA synthetase class IIc family protein)

HSP 1 Score: 602.4 bits (1552), Expect = 1.7e-172
Identity = 281/343 (81.92%), Postives = 312/343 (90.96%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           MQLH+RD HP+GILKN IY+YFD+NYSNKF KF+DLSPIV+ K+NFDDVLVP DHVSRS 
Sbjct: 87  MQLHRRDKHPIGILKNAIYDYFDSNYSNKFEKFEDLSPIVTTKQNFDDVLVPADHVSRSL 146

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYY+DSQTVLRCHTSAHQAELLR+G+++FLVTGDVYRRDSIDSTHYPVFHQMEG  VF
Sbjct: 147 NDTYYVDSQTVLRCHTSAHQAELLRKGHSRFLVTGDVYRRDSIDSTHYPVFHQMEGFCVF 206

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           S  DW  SG D T +AA+DLKKCLEGLARHLFG+VEMRWVDTYFPFTNPSFELEI+F+E 
Sbjct: 207 SPEDWNGSGKDSTLYAAEDLKKCLEGLARHLFGSVEMRWVDTYFPFTNPSFELEIYFKED 266

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGV EQ ILK+S  ENNVAWAFGLGLERLAMVLFDIPDIR FWS+DERFT+QF 
Sbjct: 267 WLEVLGCGVTEQVILKQSGLENNVAWAFGLGLERLAMVLFDIPDIRFFWSSDERFTSQFG 326

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
           KG+LGVKFK +SK+PPCYKDISFWI+D FTENN CEVVRG+AGDLVEEV+LID FTNKKK
Sbjct: 327 KGELGVKFKPYSKYPPCYKDISFWISDLFTENNFCEVVRGIAGDLVEEVKLIDQFTNKKK 386

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           G+TSHCYRI +RSMERSLTDEE+NDLQ  VR++VQ KLNV LR
Sbjct: 387 GLTSHCYRIVFRSMERSLTDEEVNDLQSKVRDEVQKKLNVELR 429

BLAST of CmoCh03G014890 vs. TAIR10
Match: AT4G39280.1 (AT4G39280.1 phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative)

HSP 1 Score: 65.5 bits (158), Expect = 7.5e-11
Identity = 55/200 (27.50%), Postives = 92/200 (46.00%), Query Frame = 1

Query: 51  VPEDHV-------------SRSYNDTYYID--SQTVLRCHTSAHQAELL----RRGYT-- 110
           +PED+V             SR YN  +  +  ++ +LR HT+A  + +L    ++ +   
Sbjct: 277 LPEDYVERVKRVHESGGYGSRGYNYDWKREEANKNLLRTHTTAVSSRMLYALAQKPFVPK 336

Query: 111 QFLVTGDVYRRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLAR 170
           ++     V+R +++D TH   FHQ+EG+                     DL   LE    
Sbjct: 337 KYFSIDRVFRNEAVDRTHLAEFHQIEGL------------ICDRGLTLGDLIGVLEDFFS 396

Query: 171 HLFGAVEMRWVDTYFPFTNPSFELEIFFQ--EKWLEVLGCGVMEQEILKRSDKENNV-AW 227
            L G  ++R+   Y P+T PS E+  + +   KW+E+   G+   E+L       +V   
Sbjct: 397 RL-GMSKLRFKPAYNPYTEPSMEIFSYHEGLGKWVEIGNSGMFRPEMLLPMGLPEDVRVI 456

BLAST of CmoCh03G014890 vs. NCBI nr
Match: gi|659077965|ref|XP_008439478.1| (PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo])

HSP 1 Score: 668.3 bits (1723), Expect = 7.1e-189
Identity = 321/343 (93.59%), Postives = 332/343 (96.79%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           MQLH+RDNHPLGILKNEIYNYFDTNYSNKF KFDDLSPIVSVKENFDDVLVPEDHVSRSY
Sbjct: 89  MQLHRRDNHPLGILKNEIYNYFDTNYSNKFIKFDDLSPIVSVKENFDDVLVPEDHVSRSY 148

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQME VRVF
Sbjct: 149 NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEAVRVF 208

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           SLND E SGTDGTS+AADDLKKCLEGLARHLFGAVEMRWVD YFPFT+PSFELEIFFQEK
Sbjct: 209 SLNDLEVSGTDGTSYAADDLKKCLEGLARHLFGAVEMRWVDAYFPFTDPSFELEIFFQEK 268

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGVMEQ+ILK+  KEN+VAWAFGLGLERLAMVLF IPD+RLFWSADERFT+QFS
Sbjct: 269 WLEVLGCGVMEQKILKKCGKENHVAWAFGLGLERLAMVLFGIPDVRLFWSADERFTSQFS 328

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
           KGKLGVKFK FSKFPPCYKDISFWIN+SFTENNLCEVVRG+AGDLVEEVQLIDSFTN+KK
Sbjct: 329 KGKLGVKFKPFSKFPPCYKDISFWINESFTENNLCEVVRGIAGDLVEEVQLIDSFTNEKK 388

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           GMTSHCYRIAYRSMERSLTDEEINDLQ NVREQVQSKL VVLR
Sbjct: 389 GMTSHCYRIAYRSMERSLTDEEINDLQRNVREQVQSKLKVVLR 431

BLAST of CmoCh03G014890 vs. NCBI nr
Match: gi|449433501|ref|XP_004134536.1| (PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus])

HSP 1 Score: 665.6 bits (1716), Expect = 4.6e-188
Identity = 319/343 (93.00%), Postives = 332/343 (96.79%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           MQLH+RDNHPLGILKNEIYNYFDTNYSNKF KFDDLSPIVSVKENFDDVLVPEDHVSRSY
Sbjct: 89  MQLHRRDNHPLGILKNEIYNYFDTNYSNKFIKFDDLSPIVSVKENFDDVLVPEDHVSRSY 148

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQME VRVF
Sbjct: 149 NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEAVRVF 208

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           SL+D E SGTDGTS+AADDLKKCLEGLARHLFGAVEMRWVD YFPFT+PSFELEIFFQEK
Sbjct: 209 SLDDLEVSGTDGTSYAADDLKKCLEGLARHLFGAVEMRWVDAYFPFTDPSFELEIFFQEK 268

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGVMEQ+ILK+  KEN+V+WAFGLGLERLAMVLF IPD+RLFWSADERFT+QFS
Sbjct: 269 WLEVLGCGVMEQKILKKCGKENHVSWAFGLGLERLAMVLFGIPDVRLFWSADERFTSQFS 328

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
           KGKLGVKFK FSKFPPCYKDISFWIN+SFTENNLCEVVRG+AGDLVEEVQLIDSFTN+KK
Sbjct: 329 KGKLGVKFKPFSKFPPCYKDISFWINESFTENNLCEVVRGIAGDLVEEVQLIDSFTNEKK 388

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           G TSHCYRIAYRSMERSLTDEEINDLQ NVREQVQSKLNVVLR
Sbjct: 389 GTTSHCYRIAYRSMERSLTDEEINDLQTNVREQVQSKLNVVLR 431

BLAST of CmoCh03G014890 vs. NCBI nr
Match: gi|657982497|ref|XP_008383288.1| (PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Malus domestica])

HSP 1 Score: 637.5 bits (1643), Expect = 1.3e-179
Identity = 304/343 (88.63%), Postives = 322/343 (93.88%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           +QLH+RD HPLGILKN IY YFDTNYSN+F KFD+L PIVSVKENFDDVLVP DHVSRSY
Sbjct: 90  LQLHRRDQHPLGILKNAIYEYFDTNYSNQFDKFDNLCPIVSVKENFDDVLVPADHVSRSY 149

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYYIDSQTVLRCHTSAHQAE+LRRG+T FLVTGDVYRRDSIDSTHYPVFHQMEGVRVF
Sbjct: 150 NDTYYIDSQTVLRCHTSAHQAEILRRGHTHFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 209

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           S  DWEASGTDGTS+AA DLKKCLEGLARHLFG+VEMRWVDTYFPFTNPSFELEIFFQEK
Sbjct: 210 SPQDWEASGTDGTSYAAGDLKKCLEGLARHLFGSVEMRWVDTYFPFTNPSFELEIFFQEK 269

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGV EQEIL+RS K +NVAWAFGLGLERLAMVLFDIPDIRLFWS DERFT+QFS
Sbjct: 270 WLEVLGCGVTEQEILRRSGKTDNVAWAFGLGLERLAMVLFDIPDIRLFWSTDERFTSQFS 329

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
            G+LG+KFK FSK+PPCYKD+SFWIN+SFTENNLCEVVR VAGDL EEVQLID+FTN KK
Sbjct: 330 SGQLGIKFKPFSKYPPCYKDVSFWINESFTENNLCEVVREVAGDLAEEVQLIDNFTN-KK 389

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           GMTSHCYRIAYRSMERSLTD+EIN LQWNVRE VQSKLNVVLR
Sbjct: 390 GMTSHCYRIAYRSMERSLTDDEINKLQWNVRELVQSKLNVVLR 431

BLAST of CmoCh03G014890 vs. NCBI nr
Match: gi|566200587|ref|XP_006376212.1| (phenylalanyl-tRNA synthetase class IIc family protein [Populus trichocarpa])

HSP 1 Score: 636.7 bits (1641), Expect = 2.3e-179
Identity = 303/343 (88.34%), Postives = 325/343 (94.75%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           +QLH+RD HPLGILKN IY+YFDTNYSNKF KFD+L PIVSVK+NFDDVLVP DHVSRSY
Sbjct: 85  LQLHRRDQHPLGILKNTIYDYFDTNYSNKFDKFDELCPIVSVKQNFDDVLVPADHVSRSY 144

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYY+DSQTVLRCHTSAHQAELLR+G+T FLVTGDVYRRDSIDSTHYPVFHQMEG RVF
Sbjct: 145 NDTYYVDSQTVLRCHTSAHQAELLRKGHTHFLVTGDVYRRDSIDSTHYPVFHQMEGFRVF 204

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           S ++WEASG DGTSFAA+DLKKCLEGLA HLFGAVEMRWVDTYFPFTNPSFELEI+FQEK
Sbjct: 205 SPDEWEASGVDGTSFAAEDLKKCLEGLATHLFGAVEMRWVDTYFPFTNPSFELEIYFQEK 264

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGV EQEILKR+ K NNVAWAFGLGLERLAMVLFDIPDIRLFWS DERFT+QFS
Sbjct: 265 WLEVLGCGVTEQEILKRNGKLNNVAWAFGLGLERLAMVLFDIPDIRLFWSNDERFTSQFS 324

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
           KG+LG+KFK FSK+PPCYKD+SFWIN+SFTENNLCEVVRG+AGDLVEEV LID+FTN KK
Sbjct: 325 KGQLGIKFKPFSKYPPCYKDMSFWINESFTENNLCEVVRGIAGDLVEEVSLIDNFTN-KK 384

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           GMTSHCYRIAYRSMERSLTDEEINDLQ +VREQVQSKLNVVLR
Sbjct: 385 GMTSHCYRIAYRSMERSLTDEEINDLQMSVREQVQSKLNVVLR 426

BLAST of CmoCh03G014890 vs. NCBI nr
Match: gi|1009129841|ref|XP_015881985.1| (PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Ziziphus jujuba])

HSP 1 Score: 636.7 bits (1641), Expect = 2.3e-179
Identity = 301/343 (87.76%), Postives = 323/343 (94.17%), Query Frame = 1

Query: 1   MQLHQRDNHPLGILKNEIYNYFDTNYSNKFTKFDDLSPIVSVKENFDDVLVPEDHVSRSY 60
           MQLH+RD HPLGILKN IY YFDT+YSNKF KFDDL PIVSVKENFDDVLVP DHVSRSY
Sbjct: 91  MQLHRRDKHPLGILKNAIYEYFDTSYSNKFDKFDDLCPIVSVKENFDDVLVPADHVSRSY 150

Query: 61  NDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 120
           NDTYY+DS+TVLRCHTSAHQAELLRRG+T FLVTGDVYRRDSIDSTHYPVFHQMEGVRVF
Sbjct: 151 NDTYYVDSETVLRCHTSAHQAELLRRGHTHFLVTGDVYRRDSIDSTHYPVFHQMEGVRVF 210

Query: 121 SLNDWEASGTDGTSFAADDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 180
           S  DW  SG D TS+AA+DLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK
Sbjct: 211 SPEDWSESGIDATSYAAEDLKKCLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIFFQEK 270

Query: 181 WLEVLGCGVMEQEILKRSDKENNVAWAFGLGLERLAMVLFDIPDIRLFWSADERFTTQFS 240
           WLEVLGCGV EQEILKRS K +NVAWAFGLGLERLAM+LFDIPDIRLFWS+DERFT+QFS
Sbjct: 271 WLEVLGCGVTEQEILKRSGKADNVAWAFGLGLERLAMILFDIPDIRLFWSSDERFTSQFS 330

Query: 241 KGKLGVKFKSFSKFPPCYKDISFWINDSFTENNLCEVVRGVAGDLVEEVQLIDSFTNKKK 300
            G+LG+KFK FSK+PPCYKD+SFWIN+SFTENNLCEVVRG+AGDLVEEV+LID+FTN KK
Sbjct: 331 TGQLGIKFKPFSKYPPCYKDVSFWINESFTENNLCEVVRGIAGDLVEEVRLIDNFTN-KK 390

Query: 301 GMTSHCYRIAYRSMERSLTDEEINDLQWNVREQVQSKLNVVLR 344
           GMTSHCYRIAYRSMERSLTDEEIN LQW+VREQVQ+KLNVV+R
Sbjct: 391 GMTSHCYRIAYRSMERSLTDEEINKLQWDVREQVQNKLNVVMR 432

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SYFM_ARATH3.0e-17181.92Phenylalanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana ... [more]
SYFM_DICDI2.1e-10049.36Phenylalanine--tRNA ligase, mitochondrial OS=Dictyostelium discoideum GN=mpheS P... [more]
SYFM_MOUSE2.7e-9548.65Phenylalanine--tRNA ligase, mitochondrial OS=Mus musculus GN=Fars2 PE=1 SV=1[more]
SYFM_HUMAN8.7e-9447.03Phenylalanine--tRNA ligase, mitochondrial OS=Homo sapiens GN=FARS2 PE=1 SV=1[more]
SYFM_RAT7.3e-9345.52Phenylalanine--tRNA ligase, mitochondrial OS=Rattus norvegicus GN=Fars2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
E5GCL5_CUCME5.0e-18993.59Phenylalanyl-tRNA synthetase OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0KJ29_CUCSA3.2e-18893.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G526470 PE=4 SV=1[more]
B9MWE6_POPTR1.6e-17988.34Phenylalanyl-tRNA synthetase class IIc family protein OS=Populus trichocarpa GN=... [more]
M5WGS0_PRUPE3.6e-17988.34Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006022mg PE=4 SV=1[more]
A0A151TD69_CAJCA5.2e-17887.76Uncharacterized protein OS=Cajanus cajan GN=KK1_019602 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G58140.11.7e-17281.92 phenylalanyl-tRNA synthetase class IIc family protein[more]
AT4G39280.17.5e-1127.50 phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase,... [more]
Match NameE-valueIdentityDescription
gi|659077965|ref|XP_008439478.1|7.1e-18993.59PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo... [more]
gi|449433501|ref|XP_004134536.1|4.6e-18893.00PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sati... [more]
gi|657982497|ref|XP_008383288.1|1.3e-17988.63PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Malus domest... [more]
gi|566200587|ref|XP_006376212.1|2.3e-17988.34phenylalanyl-tRNA synthetase class IIc family protein [Populus trichocarpa][more]
gi|1009129841|ref|XP_015881985.1|2.3e-17987.76PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Ziziphus juj... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002319Phenylalanyl-tRNA_Synthase
IPR004530Phe-tRNA-synth_IIc_mito
IPR005121Fdx_antiC-bd
IPR006195aa-tRNA-synth_II
Vocabulary: Molecular Function
TermDefinition
GO:0000049tRNA binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0005524ATP binding
GO:0000166nucleotide binding
GO:0004826phenylalanine-tRNA ligase activity
GO:0000287magnesium ion binding
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Biological Process
TermDefinition
GO:0043039tRNA aminoacylation
GO:0006432phenylalanyl-tRNA aminoacylation
GO:0008033tRNA processing
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process GO:0009094 L-phenylalanine biosynthetic process
biological_process GO:0006432 phenylalanyl-tRNA aminoacylation
biological_process GO:0048481 plant ovule development
biological_process GO:0008033 tRNA processing
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0006571 tyrosine biosynthetic process
biological_process GO:0043039 tRNA aminoacylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005739 mitochondrion
cellular_component GO:0009328 phenylalanine-tRNA ligase complex
cellular_component GO:0016020 membrane
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0005524 ATP binding
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004826 phenylalanine-tRNA ligase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G014890.1CmoCh03G014890.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002319Phenylalanyl-tRNA synthetasePFAMPF01409tRNA-synt_2dcoord: 5..239
score: 5.3
IPR004530Phenylalanyl-tRNA synthetase, class IIc, mitochondrialTIGRFAMsTIGR00469TIGR00469coord: 154..340
score: 2.6
IPR005121Phenylalanine-tRNA ligase, beta subunit, ferrodoxin-fold anticodon-bindingGENE3DG3DSA:3.30.70.380coord: 256..343
score: 9.0
IPR005121Phenylalanine-tRNA ligase, beta subunit, ferrodoxin-fold anticodon-bindingPFAMPF03147FDX-ACBcoord: 252..343
score: 2.0
IPR005121Phenylalanine-tRNA ligase, beta subunit, ferrodoxin-fold anticodon-bindingSMARTSM00896FDX_ACB_2coord: 252..343
score: 1.9
IPR005121Phenylalanine-tRNA ligase, beta subunit, ferrodoxin-fold anticodon-bindingPROFILEPS51447FDX_ACBcoord: 252..343
score: 25
IPR005121Phenylalanine-tRNA ligase, beta subunit, ferrodoxin-fold anticodon-bindingunknownSSF54991Anticodon-binding domain of PheRScoord: 247..343
score: 3.01
IPR006195Aminoacyl-tRNA synthetase, class IIPROFILEPS50862AA_TRNA_LIGASE_IIcoord: 95..256
score: 12
NoneNo IPR availableGENE3DG3DSA:3.30.930.10coord: 2..240
score: 5.5
NoneNo IPR availablePANTHERPTHR11538PHENYLALANYL-TRNA SYNTHETASEcoord: 1..334
score: 2.2E
NoneNo IPR availablePANTHERPTHR11538:SF41PHENYLALANINE--TRNA LIGASE, MITOCHONDRIALcoord: 1..334
score: 2.2E
NoneNo IPR availableunknownSSF55681Class II aaRS and biotin synthetasescoord: 3..239
score: 1.16