CmoCh03G011870 (gene) Cucurbita moschata (Rifu)

NameCmoCh03G011870
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionThreonine dehydratase (4.3.1.19) (Threonine deaminase)
LocationCmo_Chr03 : 9271183 .. 9273828 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAAAAAAAACTGAATTCCAACATCCTCTGTTTTGTTTCTCCAACAATTATGGAATCCATTCTTCTCACCGCCACCACTTCAAACCTCCCGCTGCCTCGAAACTCTTCCCTTTCCCCACTCTCTTTCTCCTCCTCCCTCCCGAATCAGGTGAGCACCAAGGTCGACATCAGGCGAACCAACAAAGGTACGACCAAGGCCCCGCGCATAGTGGCCTCCTCCTCGGGGTCCAAAGGAAGGGCGGGCAAAGGGGTGTCTGCCAATGTAGCAGTTGACGGTTCGGTTGCCGCGGTTGGGGCGGAGGCTGTGAAGGACGTGTCGTGGAAGGAACTACAGTACGAGGAAGGGTCAACCGGGCATAGGCAGCCCCTTCCCGCTGACCCGGATACTGAGAAGCAGATGGAATATATTACAAATATATTGGGTTCAAATGTGTACGATGTGGCTATTGAATCCCCATTGCAGCTTGCTCCCCTCTTGTCTACTCAATTGGGTGTCAATCTGTGGCTCAAGAGGGAGGATTCACAGCAGGTACGACAAAAATGGGTACAGAGAAGAAAAAGGGTAACGAGAAGAATTGAAGTTAGAAATGATTAATATTTTGTTTATGTAACGTAGGTGTTCTCATTCAAGATTCGAGGCGCTTACAATATGATTTCCCGACTTTCTGAAGAAAAATTGAAAAAGGGGATTATCTGTGCGTCAGCTGGGAATCATGCCCAGGGAGTTGCATTGGCTGGTCAGCGATTAAAAACCAAAGCCTACATTGTTATGCCAACAACTACTCCTCAAATCAAGGTCTGGAATACTAACTGTTATTTATTTATTTATGTATTTCAAAATTTGAGGTTTGAAATTAACTTTGAATCATGAAAACATATAGATAGATGCAGTTGAGAGATTGGATGGAATTGTTGATCTGTATGGCAATTCTTTTGATGAAGCGCAAAAAAGAGCTAAGGAGAGGAGCGAAGAGGAAGGCCTCACATTCATACCTCCTTTTGATGACAAAGATGTTATAGCAGGGCAAGGTACAGTCGGTATGGAAATTGGGCGTCAAATAAGGGGCAAAATCCATGCTATTTTTGTTCCTATTGGCGGTGGTGGTCTCGCTTCTGGGATTGTCTCTTTTTACAAGCTGGTTTATCCTGATGTAAGTGCCTCCATATGTGTTCCTTGTTCTTGTTGTTGTTGTTGATTAAATTTTGGGTTGGTTGATTAGTGTTGTTGTGTTGATGATACAGGTAAAGGTATTTGGGGTGGAACCAAATGATCAGAACTCCATGGCGCAGGCATTGTATCGTGGGGAGATAGTGAATGTGACAGATATTGGGCATTTTGCAGATGGAGTAGCTGTTCAACAAGTAGGCGAAGAGACATTTCGAATCTGCTATGAACTCCTGGATGATGTGATTCTTGTTAAAAAGGAATCAATATCTGCGGCTATAAAGGTTTGGTTGGATTAATTCTGTGCTACTTCCAATAATGATTCATAATATTGTTCTAAGAGAGATACCTGGTTGTATGCATGTGACAGGACATGTTTAACGACGGCAGGAACATCTTAGAACCTTCCGGTGCTCTTTCCATTGCTGGAGCCAAGGCCTACTGCGAGTATAACAACATTAAGGGAGTAAATATTGTGGCTGTTACCAGTGGTGCAAATATGAACTTTGACCAACTGGGACAAATCTCTGATCTTGCCAATATTGATCAGTCTATTCTCGCAACTATGTTGCCAGAAACACCTGGAAGCTTAAAACAACTTACCGACCTGATCGCCGATGTGAGTTCGTTCGTTCGTTCATTCGTTCGTTCCTTCCTGCTAAATCACTTGTTTAGAATTTGACATATGATTAGACGATATATTATCTTAAATACTTGTTTTCGTTCGTTTAATGGGGTGAGTTTGGTTTTACAGTTGGGTGTCACCATTGCAGAAATGACTTATAGATTTAGCTCTGGCTCAACAGATGCTCTTGTTGTTTATAAGTAAGTGCTTCTTGAAGCTTATTTAGCATTGGTTCTTGCTTTTTCCTTCTAATATTTAACTTGGTTTATGCAATGGTATGCAGAGTTAAAGCAGTAGATGCAGCACTTGAAACTTTGGTGAGACAATTGGACTTTTTCGGGTTCAAAACTTACACTTTATCCGATAATGAAGTTGTTAGAAACCATCTCCGTTACATGGTAAATATGTATACATATATCGTTCTTGAATTAAATTTGGAACATGTCTCAATTTCTTTGTAATGAACACTTTGATTTGTCATTTCCAGATGGGAGGCAGACAAAACGTTGAAAACGAAACTCTCTTCCGTTTTACTTTCCCAGAAAAGGTTGGTGCTCTAAAGCACTTCCTCAAAGACTTTCAGCCAAACTGGAACGTTTCTTTGTTCCACCATCGTCACCAGGTATATGTTCGAGTCTCGAGTTGCTTATTAGACCCTGTTTTGATGATCATTTGATGGTTGATTCTATTGGGTAATGTTTTTGTTGAACAGGGTATTCTTACTTCAGATGTGTTGATTGGAGTCCAACTTGAGAAATCTGAGGAGAAAAAGTTTGATGAGTATGCAAAGAGGGTTGGGTATCAATATGAGGTAATATCTCAAGATGATTCAGCCCACGTTCTAGCAAAACTCTAG

mRNA sequence

GCAAAAAAAACTGAATTCCAACATCCTCTGTTTTGTTTCTCCAACAATTATGGAATCCATTCTTCTCACCGCCACCACTTCAAACCTCCCGCTGCCTCGAAACTCTTCCCTTTCCCCACTCTCTTTCTCCTCCTCCCTCCCGAATCAGGTGAGCACCAAGGTCGACATCAGGCGAACCAACAAAGGTACGACCAAGGCCCCGCGCATAGTGGCCTCCTCCTCGGGGTCCAAAGGAAGGGCGGGCAAAGGGGTGTCTGCCAATGTAGCAGTTGACGGTTCGGTTGCCGCGGTTGGGGCGGAGGCTGTGAAGGACGTGTCGTGGAAGGAACTACAGTACGAGGAAGGGTCAACCGGGCATAGGCAGCCCCTTCCCGCTGACCCGGATACTGAGAAGCAGATGGAATATATTACAAATATATTGGGTTCAAATGTGTACGATGTGGCTATTGAATCCCCATTGCAGCTTGCTCCCCTCTTGTCTACTCAATTGGGTGTCAATCTGTGGCTCAAGAGGGAGGATTCACAGCAGGTGTTCTCATTCAAGATTCGAGGCGCTTACAATATGATTTCCCGACTTTCTGAAGAAAAATTGAAAAAGGGGATTATCTGTGCGTCAGCTGGGAATCATGCCCAGGGAGTTGCATTGGCTGGTCAGCGATTAAAAACCAAAGCCTACATTGTTATGCCAACAACTACTCCTCAAATCAAGATAGATGCAGTTGAGAGATTGGATGGAATTGTTGATCTGTATGGCAATTCTTTTGATGAAGCGCAAAAAAGAGCTAAGGAGAGGAGCGAAGAGGAAGGCCTCACATTCATACCTCCTTTTGATGACAAAGATGTTATAGCAGGGCAAGGTACAGTCGGTATGGAAATTGGGCGTCAAATAAGGGGCAAAATCCATGCTATTTTTGTTCCTATTGGCGGTGGTGGTCTCGCTTCTGGGATTGTCTCTTTTTACAAGCTGGTTTATCCTGATGTAAAGGTATTTGGGGTGGAACCAAATGATCAGAACTCCATGGCGCAGGCATTGTATCGTGGGGAGATAGTGAATGTGACAGATATTGGGCATTTTGCAGATGGAGTAGCTGTTCAACAAGTAGGCGAAGAGACATTTCGAATCTGCTATGAACTCCTGGATGATGTGATTCTTGTTAAAAAGGAATCAATATCTGCGGCTATAAAGGACATGTTTAACGACGGCAGGAACATCTTAGAACCTTCCGGTGCTCTTTCCATTGCTGGAGCCAAGGCCTACTGCGAGTATAACAACATTAAGGGAGTAAATATTGTGGCTGTTACCAGTGGTGCAAATATGAACTTTGACCAACTGGGACAAATCTCTGATCTTGCCAATATTGATCAGTCTATTCTCGCAACTATGTTGCCAGAAACACCTGGAAGCTTAAAACAACTTACCGACCTGATCGCCGATTTGGGTGTCACCATTGCAGAAATGACTTATAGATTTAGCTCTGGCTCAACAGATGCTCTTGTTGTTTATAAAGTTAAAGCAGTAGATGCAGCACTTGAAACTTTGGTGAGACAATTGGACTTTTTCGGGTTCAAAACTTACACTTTATCCGATAATGAAGTTGTTAGAAACCATCTCCGTTACATGGTAAATATGTATACATATATCATGGGAGGCAGACAAAACGTTGAAAACGAAACTCTCTTCCGTTTTACTTTCCCAGAAAAGGTTGGTGCTCTAAAGCACTTCCTCAAAGACTTTCAGCCAAACTGGAACGTTTCTTTGTTCCACCATCGTCACCAGGTATATGGTATTCTTACTTCAGATGTGTTGATTGGAGTCCAACTTGAGAAATCTGAGGAGAAAAAGTTTGATGAGTATGCAAAGAGGGTTGGGTATCAATATGAGGTAATATCTCAAGATGATTCAGCCCACGTTCTAGCAAAACTCTAG

Coding sequence (CDS)

ATGGAATCCATTCTTCTCACCGCCACCACTTCAAACCTCCCGCTGCCTCGAAACTCTTCCCTTTCCCCACTCTCTTTCTCCTCCTCCCTCCCGAATCAGGTGAGCACCAAGGTCGACATCAGGCGAACCAACAAAGGTACGACCAAGGCCCCGCGCATAGTGGCCTCCTCCTCGGGGTCCAAAGGAAGGGCGGGCAAAGGGGTGTCTGCCAATGTAGCAGTTGACGGTTCGGTTGCCGCGGTTGGGGCGGAGGCTGTGAAGGACGTGTCGTGGAAGGAACTACAGTACGAGGAAGGGTCAACCGGGCATAGGCAGCCCCTTCCCGCTGACCCGGATACTGAGAAGCAGATGGAATATATTACAAATATATTGGGTTCAAATGTGTACGATGTGGCTATTGAATCCCCATTGCAGCTTGCTCCCCTCTTGTCTACTCAATTGGGTGTCAATCTGTGGCTCAAGAGGGAGGATTCACAGCAGGTGTTCTCATTCAAGATTCGAGGCGCTTACAATATGATTTCCCGACTTTCTGAAGAAAAATTGAAAAAGGGGATTATCTGTGCGTCAGCTGGGAATCATGCCCAGGGAGTTGCATTGGCTGGTCAGCGATTAAAAACCAAAGCCTACATTGTTATGCCAACAACTACTCCTCAAATCAAGATAGATGCAGTTGAGAGATTGGATGGAATTGTTGATCTGTATGGCAATTCTTTTGATGAAGCGCAAAAAAGAGCTAAGGAGAGGAGCGAAGAGGAAGGCCTCACATTCATACCTCCTTTTGATGACAAAGATGTTATAGCAGGGCAAGGTACAGTCGGTATGGAAATTGGGCGTCAAATAAGGGGCAAAATCCATGCTATTTTTGTTCCTATTGGCGGTGGTGGTCTCGCTTCTGGGATTGTCTCTTTTTACAAGCTGGTTTATCCTGATGTAAAGGTATTTGGGGTGGAACCAAATGATCAGAACTCCATGGCGCAGGCATTGTATCGTGGGGAGATAGTGAATGTGACAGATATTGGGCATTTTGCAGATGGAGTAGCTGTTCAACAAGTAGGCGAAGAGACATTTCGAATCTGCTATGAACTCCTGGATGATGTGATTCTTGTTAAAAAGGAATCAATATCTGCGGCTATAAAGGACATGTTTAACGACGGCAGGAACATCTTAGAACCTTCCGGTGCTCTTTCCATTGCTGGAGCCAAGGCCTACTGCGAGTATAACAACATTAAGGGAGTAAATATTGTGGCTGTTACCAGTGGTGCAAATATGAACTTTGACCAACTGGGACAAATCTCTGATCTTGCCAATATTGATCAGTCTATTCTCGCAACTATGTTGCCAGAAACACCTGGAAGCTTAAAACAACTTACCGACCTGATCGCCGATTTGGGTGTCACCATTGCAGAAATGACTTATAGATTTAGCTCTGGCTCAACAGATGCTCTTGTTGTTTATAAAGTTAAAGCAGTAGATGCAGCACTTGAAACTTTGGTGAGACAATTGGACTTTTTCGGGTTCAAAACTTACACTTTATCCGATAATGAAGTTGTTAGAAACCATCTCCGTTACATGGTAAATATGTATACATATATCATGGGAGGCAGACAAAACGTTGAAAACGAAACTCTCTTCCGTTTTACTTTCCCAGAAAAGGTTGGTGCTCTAAAGCACTTCCTCAAAGACTTTCAGCCAAACTGGAACGTTTCTTTGTTCCACCATCGTCACCAGGTATATGGTATTCTTACTTCAGATGTGTTGATTGGAGTCCAACTTGAGAAATCTGAGGAGAAAAAGTTTGATGAGTATGCAAAGAGGGTTGGGTATCAATATGAGGTAATATCTCAAGATGATTCAGCCCACGTTCTAGCAAAACTCTAG
BLAST of CmoCh03G011870 vs. Swiss-Prot
Match: THD1_ARATH (Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana GN=OMR1 PE=1 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 5.2e-153
Identity = 285/536 (53.17%), Postives = 384/536 (71.64%), Query Frame = 1

Query: 89  VSWKELQYEEGSTG---HRQPLPADPDTEKQMEYITNILGSNVYDVAIESPLQLAPLLST 148
           VS   LQY  G  G    R     +    + MEY+TNIL + VYD+AIESPLQLA  LS 
Sbjct: 62  VSPNSLQYPAGYLGAVPERTNEAENGSIAEAMEYLTNILSTKVYDIAIESPLQLAKKLSK 121

Query: 149 QLGVNLWLKREDSQQVFSFKIRGAYNMISRLSEEKLKKGIICASAGNHAQGVALAGQRLK 208
           +LGV ++LKRED Q VFSFK+RGAYNM+ +L  ++L KG+IC+SAGNHAQGVAL+  +L 
Sbjct: 122 RLGVRMYLKREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQGVALSASKLG 181

Query: 209 TKAYIVMPTTTPQIKIDAVERLDGIVDLYGNSFDEAQKRAKERSEEEGLTFIPPFDDKDV 268
             A IVMP TTP+IK  AVE L   V L+G+S+D+AQ  AK R+EEEGLTFIPPFD  DV
Sbjct: 182 CTAVIVMPVTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTFIPPFDHPDV 241

Query: 269 IAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPDVKVFGVEPNDQNSMA 328
           IAGQGTVGMEI RQ +G +HAIFVP+GGGGL +GI ++ K V P+VK+ GVEP D N+MA
Sbjct: 242 IAGQGTVGMEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMA 301

Query: 329 QALYRGEIVNVTDIGHFADGVAVQQVGEETFRICYELLDDVILVKKESISAAIKDMFNDG 388
            +L+ GE V +  +G FADGVAV++VGEETFRI   L+D V+LV +++I A+IKDMF + 
Sbjct: 302 LSLHHGERVILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEK 361

Query: 389 RNILEPSGALSIAGAKAYCEYNNIKGVNIVAVTSGANMNFDQLGQISDLANI---DQSIL 448
           RNILEP+GAL++AGA+AYC+Y  +K VN+VA+TSGANMNFD+L  +++LAN+    +++L
Sbjct: 362 RNILEPAGALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVL 421

Query: 449 ATMLPETPGSLKQLTDLIADLGVTIAEMTYRFSSGSTDALVVYKVKAVDAA-LETLVRQL 508
           AT++PE PGS KQ  +L+  +   I+E  YR SS   +A+V+Y V    A  L+ L +++
Sbjct: 422 ATLMPEKPGSFKQFCELVGPM--NISEFKYRCSS-EKEAVVLYSVGVHTAGELKALQKRM 481

Query: 509 DFFGFKTYTLSDNEVVRNHLRYMVNMYTYIMGGRQNVENETLFRFTFPEKVGALKHFLKD 568
           +    KT  L+ +++V++HLR       Y+MGGR  V +E L RFTFPE+ GAL +FL  
Sbjct: 482 ESSQLKTVNLTTSDLVKDHLR-------YLMGGRSTVGDEVLCRFTFPERPGALMNFLDS 541

Query: 569 FQPNWNVSLFHHRHQVYGILTSDVLIGVQLEKSEEKKFDEYAKRVGYQYEVISQDD 618
           F P WN++LFH+R Q  G   ++VL+G+Q+ + E ++F   AK +GY Y ++S DD
Sbjct: 542 FSPRWNITLFHYRGQ--GETGANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDD 585

BLAST of CmoCh03G011870 vs. Swiss-Prot
Match: THD1_SOLLC (Threonine dehydratase biosynthetic, chloroplastic OS=Solanum lycopersicum GN=TD PE=1 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 3.6e-138
Identity = 257/519 (49.52%), Postives = 359/519 (69.17%), Query Frame = 1

Query: 108 PADPDTEKQMEYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIR 167
           P   D+++  +Y+ +IL S VYDVAIESPL+LA  LS +LGVN ++KRED Q+VFSFK+R
Sbjct: 86  PTGGDSDELFQYLVDILASPVYDVAIESPLELAEKLSDRLGVNFYIKREDKQRVFSFKLR 145

Query: 168 GAYNMISRLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERL 227
           GAYNM+S LS E+L KG+I ASAGNHAQGVALAGQRL   A IVMPTTTPQIKIDAV  L
Sbjct: 146 GAYNMMSNLSREELDKGVITASAGNHAQGVALAGQRLNCVAKIVMPTTTPQIKIDAVRAL 205

Query: 228 DGIVDLYGNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAI 287
            G V LYG +FDEAQ  A E SE++GL +IPPFDD  VI GQGT+G EI RQ++  IHA+
Sbjct: 206 GGDVVLYGKTFDEAQTHALELSEKDGLKYIPPFDDPGVIKGQGTIGTEINRQLK-DIHAV 265

Query: 288 FVPIGGGGLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVA 347
           F+P+GGGGL +G+ +F+K + P+ K+ GVEP    SM  +L+ G  V ++++  FADGVA
Sbjct: 266 FIPVGGGGLIAGVATFFKQIAPNTKIIGVEPYGAASMTLSLHEGHRVKLSNVDTFADGVA 325

Query: 348 VQQVGEETFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYN 407
           V  VGE TF  C EL+D ++LV  + ISAAIKD++++GRNILE SGA++IAGA AYCE+ 
Sbjct: 326 VALVGEYTFAKCQELIDGMVLVANDGISAAIKDVYDEGRNILETSGAVAIAGAAAYCEFY 385

Query: 408 NIKGVNIVAVTSGANMNFDQLGQISDLANI---DQSILATMLPETPGSLKQLTDLIADLG 467
            IK  NIVA+ SGANM+F +L ++++LA +    +++LAT + E  GS K    L+  L 
Sbjct: 386 KIKNENIVAIASGANMDFSKLHKVTELAGLGSGKEALLATFMVEQQGSFKTFVGLVGSLN 445

Query: 468 VTIAEMTYRFSSGSTDALVVYKVKA-VDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRY 527
            T  E+TYRF+S   +AL++Y+V    ++ LE ++  +      T  LS NE+V +HL++
Sbjct: 446 FT--ELTYRFTSERKNALILYRVNVDKESDLEKMIEDMKSSNMTTLNLSHNELVVDHLKH 505

Query: 528 MVNMYTYIMGGRQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTS 587
           +V       GG  N+ +E    F  PEK   LK FL  F P WN++L  +R+Q  G + +
Sbjct: 506 LV-------GGSANISDEIFGEFIVPEKAETLKTFLDAFSPRWNITLCRYRNQ--GDINA 565

Query: 588 DVLIGVQLEKSEEKKFDEYAKRVGYQYEVISQDDSAHVL 623
            +L+G Q+ ++E  +F   A ++GY YE+ + +++ +++
Sbjct: 566 SLLMGFQVPQAEMDEFKNQADKLGYPYELDNYNEAFNLV 592

BLAST of CmoCh03G011870 vs. Swiss-Prot
Match: ILVA_BURM1 (L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain ATCC 17616 / 249) GN=ilvA PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 1.4e-118
Identity = 230/500 (46.00%), Postives = 323/500 (64.60%), Query Frame = 1

Query: 118 EYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISRLS 177
           +Y+  IL + VYDVA E+ L+ A  LS +L   ++LKRED+Q VFSFK+RGAYN ++ + 
Sbjct: 5   DYLKKILTARVYDVAFETELEPARNLSARLRNPVYLKREDNQPVFSFKLRGAYNKMAHIP 64

Query: 178 EEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGI---VDLY 237
            + L +G+I ASAGNHAQGVA +  R+  KA IV+P TTPQ+K+DAV    G    V   
Sbjct: 65  ADALARGVITASAGNHAQGVAFSAARMGVKAVIVVPVTTPQVKVDAVRAHGGPGVEVIQA 124

Query: 238 GNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGG 297
           G S+ +A   A +  EE GLTF+ PFDD  VIAGQGT+ MEI RQ +G IHAIFVPIGGG
Sbjct: 125 GESYSDAYAHALKVQEERGLTFVHPFDDPYVIAGQGTIAMEILRQHQGPIHAIFVPIGGG 184

Query: 298 GLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEE 357
           GLA+G+ ++ K V P++KV GV+  D  +MAQ+L  G+ V + ++G FADG AV+ VGEE
Sbjct: 185 GLAAGVAAYVKAVRPEIKVIGVQAEDSCAMAQSLQAGKRVELAEVGLFADGTAVKLVGEE 244

Query: 358 TFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVNI 417
           TFR+C E LD V+ V  +++ AAIKD+F D R++LEPSGAL++AGAK Y E   I+   +
Sbjct: 245 TFRLCKEYLDGVVTVDTDALCAAIKDVFQDTRSVLEPSGALAVAGAKLYAEREGIENQTL 304

Query: 418 VAVTSGANMNFDQLGQISDLANID---QSILATMLPETPGSLKQLTDLIADLGVTIAEMT 477
           VAVTSGANMNFD++  +++ A +    +++ A  +PE  GS K+   L+ D  VT  E  
Sbjct: 305 VAVTSGANMNFDRMRFVAERAEVGEAREAVFAVTIPEERGSFKRFCSLVGDRNVT--EFN 364

Query: 478 YRFSSGSTDALVVYKVKAVDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYTYI 537
           YR +   +  + V            +    +  GFKT  L+ +E+ + H+RYMV      
Sbjct: 365 YRIADAQSAHIFVGVQIRRRGESADIAANFESHGFKTADLTHDELSKEHIRYMV------ 424

Query: 538 MGGRQNVE-NETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGVQ 597
            GGR  +  +E LFRF FPE+ GAL  FL    P+WN+SLFH+R+Q  G   S +L+G+Q
Sbjct: 425 -GGRSPLALDERLFRFEFPERPGALMKFLSSMAPDWNISLFHYRNQ--GADYSSILVGLQ 484

Query: 598 LEKSEEKKFDEYAKRVGYQY 611
           + +++  +F+ +   +GY Y
Sbjct: 485 VPQADHAEFERFLAALGYPY 493

BLAST of CmoCh03G011870 vs. Swiss-Prot
Match: ILVA_HAEIN (L-threonine dehydratase biosynthetic IlvA OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) GN=ilvA PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 3.4e-112
Identity = 220/508 (43.31%), Postives = 322/508 (63.39%), Query Frame = 1

Query: 116 QMEYITNI--LGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMI 175
           Q +YI  I  LGS VY+ A  +PLQ    LS +L  N+W+KRED Q V SFK+RGAY MI
Sbjct: 12  QSDYINAIVKLGSRVYEAAQVTPLQKMGKLSERLHNNIWIKREDRQPVNSFKLRGAYAMI 71

Query: 176 SRLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDL 235
           S LS E+   G+I ASAGNHAQGVAL+ ++L  KA IVMP  TP IK+DAV    G V L
Sbjct: 72  SSLSAEQKAAGVIAASAGNHAQGVALSAKQLGLKALIVMPQNTPSIKVDAVRGFGGEVLL 131

Query: 236 YGNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGG 295
           +G +FDEA+ +A E S+E+ +TFIPPFD   VIAGQGT+ ME+ +Q+   +  +FV +GG
Sbjct: 132 HGANFDEAKAKAIELSKEKNMTFIPPFDHPLVIAGQGTLAMEMLQQV-ADLDYVFVQVGG 191

Query: 296 GGLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGE 355
           GGLA+G+    K   P++K+ GVE  D   +  AL +GE  ++T IG FADGVAV+++G+
Sbjct: 192 GGLAAGVAILLKQFMPEIKIIGVESKDSACLKAALDKGEPTDLTHIGLFADGVAVKRIGD 251

Query: 356 ETFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVN 415
           ETFR+C + LDD++LV  + + AA+KD+F + R + EPSGAL +AG K Y + N+I+G N
Sbjct: 252 ETFRLCQQYLDDMVLVDSDEVCAAMKDLFENVRAVAEPSGALGLAGLKKYVKQNHIEGKN 311

Query: 416 IVAVTSGANMNFDQLGQISDLANID---QSILATMLPETPGSLKQLTDLIADLGVTIAEM 475
           + A+ SGAN+NF  L  +S+   I    +++LA  +PE PGS  +   ++ +  VT  E 
Sbjct: 312 MAAILSGANLNFHTLRYVSERCEIGENREALLAVTMPEQPGSFLKFAYVLGNRAVT--EF 371

Query: 476 TYRFSSGSTDALVVYKVKAVDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYTY 535
           +YR++      + V      +     ++  L   GF    +SD+++ + H+R       Y
Sbjct: 372 SYRYADDKRACVFVGVRTTNEQEKADIIADLTKNGFDVEDMSDDDIAKTHVR-------Y 431

Query: 536 IMGGRQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGVQ 595
           +MGGR   +NE L+ F FPE+ GAL  FL+  Q  WN+SLFH+R   +G    ++L G Q
Sbjct: 432 LMGGRAANDNERLYTFEFPEQKGALLKFLETLQNRWNISLFHYR--AHGADYGNILAGFQ 491

Query: 596 LEKSEEKKFDEYAKRVGYQYEVISQDDS 619
           +E+ E+ +F++   ++ Y +E +++  S
Sbjct: 492 IEQREQAEFEQGLAQLNYVFEDVTKSKS 507

BLAST of CmoCh03G011870 vs. Swiss-Prot
Match: ILVA_PASMU (L-threonine dehydratase biosynthetic IlvA OS=Pasteurella multocida (strain Pm70) GN=ilvA PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 9.2e-110
Identity = 219/503 (43.54%), Postives = 320/503 (63.62%), Query Frame = 1

Query: 118 EYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISRLS 177
           EY+  +L S VYDVA  +PLQ    LS +LG  +++KRED Q V SFK+RGAY MI+ LS
Sbjct: 14  EYLKAVLSSKVYDVAQVTPLQDMAKLSERLGNKVFIKREDRQPVHSFKLRGAYAMIAGLS 73

Query: 178 EEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDLYGNS 237
            E+   G+I ASAGNHAQGVAL+ + L  +A IVMP  TP IK+DAV    G V L+G +
Sbjct: 74  AEQKASGVIAASAGNHAQGVALSAKHLGLRALIVMPQNTPSIKVDAVRGFGGEVLLHGAN 133

Query: 238 FDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLA 297
           FDEA+ +A E +E + +TFIPPFD   VIAGQG++ ME+ +Q   +I  IFVP+GGGGLA
Sbjct: 134 FDEAKAKAIELAESKNMTFIPPFDHPAVIAGQGSIAMELLQQ-NSQIDRIFVPVGGGGLA 193

Query: 298 SGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEETFR 357
           +GI    K + P++KV GVE  D   + +AL  G+ +++  +G FADGVAV+++G+ETFR
Sbjct: 194 AGIAVLIKQLMPEIKVIGVESKDSACLYRALKAGKPIDLDRVGLFADGVAVKRIGDETFR 253

Query: 358 ICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVNIVAV 417
           +C + +DDV+LV  + I AA+KD+F + R I EPSGALS+AG K Y + +NI+G  +V V
Sbjct: 254 VCQQYIDDVVLVDGDEICAAVKDIFENVRAIAEPSGALSLAGLKKYVKEHNIQGETLVNV 313

Query: 418 TSGANMNFDQLGQISDLANI---DQSILATMLPETPGSLKQLTDLIADLGVTIAEMTYRF 477
            SGAN+NF  L  +S+   I    +++LA  +PE PGS  +   ++  + VT  E  YR+
Sbjct: 314 LSGANLNFHTLRYVSERCEIGEQHEALLAVTIPEQPGSFLKFCHILGHVPVT--EFKYRY 373

Query: 478 SSGSTDALVVYKVKAVDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYTYIMGG 537
           +      + V          +T++ QL   G+    LS++++ + H+RYM+       GG
Sbjct: 374 ADDKQACIFVGVRITGQEEKQTIINQLQQNGYDLIDLSNDDIAKTHVRYMI-------GG 433

Query: 538 RQNVE-NETLFRFTFPEKVGALKHFLKDF-QPNWNVSLFHHRHQVYGILTSDVLIGVQLE 597
           R N    E L+ F FPE+ GAL  FL+   Q +WN+S+FH+R   +G    +VL G QL 
Sbjct: 434 RSNSPLKERLYSFEFPEQKGALLKFLETLGQTHWNISVFHYR--AHGADYGNVLAGFQLN 493

Query: 598 KSEEKKFDEYAKRVGYQYEVISQ 616
             +   F+++ +++GY Y+ +++
Sbjct: 494 DEDLDAFNQHLEKLGYVYQDVTE 504

BLAST of CmoCh03G011870 vs. TrEMBL
Match: A0A0A0KKC3_CUCSA (Threonine dehydratase OS=Cucumis sativus GN=Csa_6G448740 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 6.8e-245
Identity = 458/631 (72.58%), Postives = 519/631 (82.25%), Query Frame = 1

Query: 1   MESILLTATTSNLPLPRNSSLSPLSFSSS-LPNQVSTKVDIRR-----TNKGTTKAPRIV 60
           MES LLT   +N  L RNSSLS LS SSS +  Q+++KV+I R      N  TT  P  +
Sbjct: 1   MESFLLT---TNPLLGRNSSLSSLSSSSSSISTQLNSKVEIIRRRIEKVNTRTTHVPASI 60

Query: 61  ASSSGSKGRAGKGVSANVAVDGSVAAVGAEAVKDVSWKELQYEEGSTGHRQPLPADPDTE 120
            +SS SK + GKG+SANVAVD SV  V  EAVKDVSW E+QYE GS GHR P PA+ D +
Sbjct: 61  VASSASKRKTGKGLSANVAVDCSV--VSEEAVKDVSWTEVQYESGSIGHRAPPPAEVDKD 120

Query: 121 KQMEYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMIS 180
           KQMEYIT ILGS VYDVA+E+PL+LAPLLSTQLGVNL+LKRED QQVFSFKIRGAYNMIS
Sbjct: 121 KQMEYITKILGSKVYDVAVETPLELAPLLSTQLGVNLYLKREDGQQVFSFKIRGAYNMIS 180

Query: 181 RLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDLY 240
           +LS++ LK G+ICASAGNHAQGVAL+ QRLK KA IVMPTTTP IK+DAV+RL G V   
Sbjct: 181 QLSKDDLKNGVICASAGNHAQGVALSAQRLKIKAQIVMPTTTPTIKVDAVKRLGGTVIQK 240

Query: 241 GNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGG 300
           G++FDE+QK AKE S   G+TFIPP+D++DVIAGQGTVGMEIGRQ+RGKIHAIFVPIGGG
Sbjct: 241 GDTFDESQKIAKEISCRCGITFIPPYDNEDVIAGQGTVGMEIGRQMRGKIHAIFVPIGGG 300

Query: 301 GLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEE 360
           GLA+GIVSFYKLVYP+VKVFGVEPND+NSMAQALYR EIVNVTDIGHFADGVAVQQVG E
Sbjct: 301 GLAAGIVSFYKLVYPEVKVFGVEPNDENSMAQALYRDEIVNVTDIGHFADGVAVQQVGNE 360

Query: 361 TFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVNI 420
            FRIC ELLDDVILV KE ISAAIKDMF+D R+ILEPSGAL+IA A+AYC+YNN+ GVN+
Sbjct: 361 NFRICRELLDDVILVTKEEISAAIKDMFSDERSILEPSGALAIAAARAYCKYNNVTGVNL 420

Query: 421 VAVTSGANMNFDQLGQISDLANIDQSILATMLPETPGSLKQLTDLIADLGVTIAEMTYRF 480
           VAV SGANMNFDQL +ISD+AN+DQSI+ TMLPETPGSLK+LT+L   LG  I EM YRF
Sbjct: 421 VAVCSGANMNFDQLREISDIANVDQSIICTMLPETPGSLKELTEL---LGENITEMQYRF 480

Query: 481 SSGSTDALVVYKVKAVDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYTYIMGG 540
           SSGS DA+VVYKV AV   LE +V  L+  GF TYTL+DN  V+NHLRYM        GG
Sbjct: 481 SSGSEDAVVVYKVSAVGKELEAMVELLNSAGFTTYTLNDNPTVKNHLRYMT-------GG 540

Query: 541 RQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGVQLEKS 600
           R ++ENETLFRFTFPE+ GALKHFLKDF+P WNVSLFHHR Q  GILTSDVLIGVQLEKS
Sbjct: 541 RADIENETLFRFTFPERKGALKHFLKDFKPTWNVSLFHHRSQ--GILTSDVLIGVQLEKS 600

Query: 601 EEKKFDEYAKRVGYQYEVISQDDSAHVLAKL 626
           E K F EY  +VGY+YEV+ QDD+AHVLAKL
Sbjct: 601 EHKHFHEYINKVGYKYEVVPQDDAAHVLAKL 614

BLAST of CmoCh03G011870 vs. TrEMBL
Match: A0A0A0KHC3_CUCSA (Threonine dehydratase OS=Cucumis sativus GN=Csa_6G448720 PE=3 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 7.2e-178
Identity = 348/636 (54.72%), Postives = 450/636 (70.75%), Query Frame = 1

Query: 1   MESILLTATTS-------NLPLPRNSSLSPLSFSSSLPNQVSTKVDIRRTNKGTTKAPRI 60
           MES+L T TTS          LP   S+ P S S  L N+  T   +RR+N  +  A   
Sbjct: 1   MESLLSTTTTSVPNSLALKSLLPSQPSIIPHS-SIQLSNRTKT---VRRSNFPSFLASLS 60

Query: 61  VASSSGSKGRAGKGVSANVAVDGSVAAVGAEAVKDVSWKELQYEEGSTGHRQPLPADPDT 120
                 +   +      N  VDGS+A V    V +VSWK+LQY  G  G     P   D 
Sbjct: 61  KLPKGSANSSSNNNTKTNAVVDGSIAIV--TGVPEVSWKDLQYPAGMLGAIPKRPEVIDE 120

Query: 121 EKQMEYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMI 180
            +QMEY+TNIL S VYDVAIESPL+LA  LSTQLG+ LWLKR+DSQ VFSFKIRGAYNM+
Sbjct: 121 RRQMEYLTNILSSKVYDVAIESPLELARKLSTQLGIQLWLKRDDSQFVFSFKIRGAYNMM 180

Query: 181 SRLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDL 240
           + L +E L++G+ICASAGNHAQGVALA  RL+T+A IVMP +TP IKI+AV  L G V L
Sbjct: 181 ANLPKEALERGVICASAGNHAQGVALAAGRLRTEAIIVMPRSTPPIKIEAVRSLGGNVLL 240

Query: 241 YGNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGG 300
           +G++FD+AQ+ A++ S+E  LT IPPFD++DVI GQGTVGMEIGRQ+RG +HAIFVP+GG
Sbjct: 241 HGDTFDDAQEHARQLSKERNLTIIPPFDNEDVIIGQGTVGMEIGRQMRGPLHAIFVPVGG 300

Query: 301 GGLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGE 360
           GGL +G+ SFYKLV+P+VK+ GVEPND NSMA AL+  ++V +  +G FADGVAV+QVG+
Sbjct: 301 GGLLAGVASFYKLVFPEVKIIGVEPNDANSMASALHNDQVVKLETVGTFADGVAVKQVGD 360

Query: 361 ETFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVN 420
           E FRI  EL+D ++LV K++I+A IKDMF D R+ILEP+GALSIAGAKAYCEYNNIKGVN
Sbjct: 361 ENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAGALSIAGAKAYCEYNNIKGVN 420

Query: 421 IVAVTSGANMNFDQLGQISDLA---NIDQSILATMLPETPGSLKQLTDLIADLGVTIAEM 480
           IVAVTSGANMNFDQLG I+D A   N  ++  AT+LPE PGSL   +DL+    VT  E 
Sbjct: 421 IVAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKPGSLITFSDLMGSRSVT--EF 480

Query: 481 TYRFSSGSTDALVVYKVKA-VDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYT 540
            YRF+S   +A+V+Y V   V + L  + ++++   F+TY L+ NE+V++HLRYM     
Sbjct: 481 KYRFNS-EKNAIVLYSVGVKVASELGEVKKKIESSPFETYDLTKNELVKDHLRYM----- 540

Query: 541 YIMGGRQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGV 600
             MGGR +V NE  +RFT PE+ GAL  FL  F P WN+SL H+R Q  GI+++DVL+G+
Sbjct: 541 --MGGRSSVPNEVFYRFTLPERSGALLQFLDAFSPRWNISLIHYRRQ--GIISADVLVGL 600

Query: 601 QLEKSEEKKFDEYAKRVGYQYEVISQDDSAHVLAKL 626
           Q+E SEE +F E A+++G+ Y V+S D ++ +L ++
Sbjct: 601 QIEGSEEAEFHESARKLGFGYVVVSNDPASKLLTQV 618

BLAST of CmoCh03G011870 vs. TrEMBL
Match: A0A0A0KII4_CUCSA (Threonine dehydratase OS=Cucumis sativus GN=Csa_6G449240 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 1.3e-158
Identity = 317/627 (50.56%), Postives = 432/627 (68.90%), Query Frame = 1

Query: 1   MESILLTATTSNLPLPRNSSL-SPLSFSSSLPNQVSTKVDIRRTNKGTTKAPRIVASSSG 60
           M+++LL A TS+ P+PR S L SP SF     N  S  +D  R     T+ P I+A+ S 
Sbjct: 1   MDALLLNAPTSH-PIPRKSFLPSPPSFF----NGSSISIDDNRNRVRLTRPPIILATLSK 60

Query: 61  SKGRAGKGVSANVAVDGSVAAVGAEAV---KDVSWKELQYEEGSTG---HRQPLPADPDT 120
                     +N +V     A+    +   K VS   LQ+  G  G    R       D+
Sbjct: 61  HALDIPPNSVSNTSVPTPADALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDS 120

Query: 121 EKQMEYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMI 180
              MEY+T ILGS VYDVAIESPLQLAP LS + GVN+WLKRED Q VFSFK+RGAYNM+
Sbjct: 121 VDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMM 180

Query: 181 SRLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDL 240
           ++L +E+L+KG+IC+SAGNHAQGVALA +RL+  A I MP TTP+IK  +V++L   V L
Sbjct: 181 AKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVL 240

Query: 241 YGNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGG 300
            G+S+DEAQ  AK+RS EEG TFIPPFD  DVIAGQGTVGMEI RQI+G +HAIFVP+GG
Sbjct: 241 VGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGG 300

Query: 301 GGLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGE 360
           GGL +GI ++ K V P+VK+ GVEP+D N+MA +L  G+ V +   G FADGVAV++VGE
Sbjct: 301 GGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGE 360

Query: 361 ETFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVN 420
           ETFR+C +L+D V+LV +++I A+IKDMF + R+ILEP+GALS+AGA+AYC+Y  +KG N
Sbjct: 361 ETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGEN 420

Query: 421 IVAVTSGANMNFDQLGQISDLANI---DQSILATMLPETPGSLKQLTDLIADLGVTIAEM 480
           +V +TSGANMNFD+L  +++LAN+    +++LAT+LPETPGS K+  +L+  + +T  E 
Sbjct: 421 VVVITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNIT--EF 480

Query: 481 TYRFSSGSTDALVVYKVKA-VDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYT 540
            YR+SS   +A+V+Y V   + + LE +  +++     TY L+ N++V++HLR       
Sbjct: 481 KYRYSS-EKEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLR------- 540

Query: 541 YIMGGRQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGV 600
           Y+MGG+ NVENE L RF FPE+ GAL+ FL    P WN++LFH  +Q  G   +++L+G+
Sbjct: 541 YLMGGKSNVENEVLCRFIFPERPGALEKFLDALSPRWNITLFH--YQSKGETGANILVGL 600

Query: 601 QLEKSEEKKFDEYAKRVGYQYEVISQD 617
           Q+EK +  +F E A+RVGY Y V++ D
Sbjct: 601 QIEKPDMGEFHERARRVGYDYVVVTDD 610

BLAST of CmoCh03G011870 vs. TrEMBL
Match: V7BJQ0_PHAVU (Threonine dehydratase OS=Phaseolus vulgaris GN=PHAVU_007G225400g PE=3 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.7e-155
Identity = 287/542 (52.95%), Postives = 388/542 (71.59%), Query Frame = 1

Query: 89  VSWKELQYEEGSTG----HRQPLPADPDTEKQMEYITNILGSNVYDVAIESPLQLAPLLS 148
           VS   LQY  G  G      +P          M Y+TNIL S VYDVAIE+PLQLAP LS
Sbjct: 70  VSPDALQYPPGYVGAVPNRSRPDSGGDGVMNAMNYLTNILTSKVYDVAIETPLQLAPKLS 129

Query: 149 TQLGVNLWLKREDSQQVFSFKIRGAYNMISRLSEEKLKKGIICASAGNHAQGVALAGQRL 208
           T+LGV +WLKRED Q VFSFKIRGAYNM+++L  E L+KG+IC+SAGNHAQGVA + +RL
Sbjct: 130 TRLGVKVWLKREDLQPVFSFKIRGAYNMMAKLPRELLEKGVICSSAGNHAQGVAFSAKRL 189

Query: 209 KTKAYIVMPTTTPQIKIDAVERLDGIVDLYGNSFDEAQKRAKERSEEEGLTFIPPFDDKD 268
              A IVMP TTP+IK  +VE L   V L G+S+DEAQ  AK+++ EEG TFIPPFD  D
Sbjct: 190 NCSAVIVMPVTTPEIKWKSVEALGAKVVLVGDSYDEAQAYAKKQAVEEGRTFIPPFDHPD 249

Query: 269 VIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPDVKVFGVEPNDQNSM 328
           VI GQGTVGMEI RQ++G +HAIFVP+GGGGL +GI ++ K V P+V++ GVEP+D N+M
Sbjct: 250 VIMGQGTVGMEIVRQMQGPLHAIFVPVGGGGLIAGIAAYVKRVNPEVRIIGVEPSDANAM 309

Query: 329 AQALYRGEIVNVTDIGHFADGVAVQQVGEETFRICYELLDDVILVKKESISAAIKDMFND 388
           A + +  + V +  +G FADGVAV++VGEETFRIC EL+D V+LV ++SI A+IKDMF +
Sbjct: 310 ALSFHHDQRVILDQVGGFADGVAVKEVGEETFRICKELVDGVVLVSRDSICASIKDMFEE 369

Query: 389 GRNILEPSGALSIAGAKAYCEYNNIKGVNIVAVTSGANMNFDQLGQISDLANI---DQSI 448
            RNILEP+GAL++AGA+AYC+YN I+G NIV +TSGANMNFD+L  +++LAN+    +++
Sbjct: 370 KRNILEPAGALALAGAEAYCKYNGIRGENIVVITSGANMNFDKLRVVTELANVGRKQEAV 429

Query: 449 LATMLPETPGSLKQLTDLIADLGVTIAEMTYRFSSGSTDALVVYKVKA-VDAALETLVRQ 508
           LAT++PE PGS KQ   L+  + +T  E  YR++S    A+V+Y V     + L  +  +
Sbjct: 430 LATIMPEEPGSFKQFCQLVGQVNIT--EFKYRYNS-DRKAVVLYSVGVHTVSELRAMQER 489

Query: 509 LDFFGFKTYTLSDNEVVRNHLRYMVNMYTYIMGGRQNVENETLFRFTFPEKVGALKHFLK 568
           ++    KTY L+++++V++HLR       Y+MGGR N++NE L RFTFPE+ GAL  FL 
Sbjct: 490 MESSQLKTYNLTESDLVKDHLR-------YLMGGRSNIQNEVLCRFTFPERPGALMKFLD 549

Query: 569 DFQPNWNVSLFHHRHQVYGILTSDVLIGVQLEKSEEKKFDEYAKRVGYQYEVISQDDSAH 623
            F P WN+SLFH+R +  G   +++L+G+Q+ KSE  +F + A R+GY Y+V++ DD   
Sbjct: 550 PFSPRWNISLFHYRGE--GETGANILVGIQVPKSEMDEFHDRANRLGYDYQVVNNDDDFQ 599

BLAST of CmoCh03G011870 vs. TrEMBL
Match: U5G6J7_POPTR (Threonine dehydratase OS=Populus trichocarpa GN=POPTR_0006s11980g PE=3 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.9e-154
Identity = 288/544 (52.94%), Postives = 393/544 (72.24%), Query Frame = 1

Query: 87  KDVSWKELQYEEGSTGH-RQPLPADPDTEK---QMEYITNILGSNVYDVAIESPLQLAPL 146
           K VS   LQY  G  G   +    D D E     MEY+TNIL S VYDVAIESPLQLAP 
Sbjct: 81  KKVSANSLQYPSGYLGAVPERTVNDGDNESIINAMEYLTNILSSKVYDVAIESPLQLAPK 140

Query: 147 LSTQLGVNLWLKREDSQQVFSFKIRGAYNMISRLSEEKLKKGIICASAGNHAQGVALAGQ 206
           LS +LGV +WLKRED Q VFSFK+RGAYNM+++L +E+L++G+IC+SAGNHAQGVALA +
Sbjct: 141 LSERLGVKVWLKREDLQPVFSFKLRGAYNMMAKLPKEQLQRGVICSSAGNHAQGVALAAK 200

Query: 207 RLKTKAYIVMPTTTPQIKIDAVERLDGIVDLYGNSFDEAQKRAKERSEEEGLTFIPPFDD 266
           RL   A I MP TTP+IK  +VERL   V L G+S+DEAQ  AK+R++EE  TFIPPFD 
Sbjct: 201 RLGCDAVIAMPVTTPEIKWQSVERLGATVVLVGDSYDEAQTYAKKRAKEEDRTFIPPFDH 260

Query: 267 KDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPDVKVFGVEPNDQN 326
            DVI GQGTVGMEI RQ++G +HAIFVP+GGGGL +GI ++ K V P+VK+ GVEP+D N
Sbjct: 261 PDVIMGQGTVGMEIVRQMQGPLHAIFVPVGGGGLIAGIAAYVKRVNPEVKIIGVEPSDAN 320

Query: 327 SMAQALYRGEIVNVTDIGHFADGVAVQQVGEETFRICYELLDDVILVKKESISAAIKDMF 386
           +MA +L+ G+ V +  +G FADGVAV++VGEETFR+C EL+D V+LV +++I A+IKDMF
Sbjct: 321 AMALSLHHGQRVMLDQVGGFADGVAVKEVGEETFRLCKELVDGVVLVSRDAICASIKDMF 380

Query: 387 NDGRNILEPSGALSIAGAKAYCEYNNIKGVNIVAVTSGANMNFDQLGQISDLANI---DQ 446
            + R+ILEP+GAL++AGA+AYC+Y  +KG N+VA+TSGANMNFD+L  +++LAN+    +
Sbjct: 381 EEKRSILEPAGALALAGAEAYCKYYGVKGANVVAITSGANMNFDKLRVVTELANVGRQQE 440

Query: 447 SILATMLPETPGSLKQLTDLIADLGVTIAEMTYRFSSGSTDALVVYKVKAVDA-ALETLV 506
           ++LAT++PE PGS K   +L+  +   I+E  YR S+   DA+V+Y V    A  LE + 
Sbjct: 441 ALLATVMPEVPGSFKHFCELVGPM--NISEFKYR-SNSEKDAVVLYSVGLHTAFELEAMK 500

Query: 507 RQLDFFGFKTYTLSDNEVVRNHLRYMVNMYTYIMGGRQNVENETLFRFTFPEKVGALKHF 566
           ++++    +TY L+ +++V++HLRY++       GG+ NV +E L RF FPE+ GAL  F
Sbjct: 501 KRMESSQLRTYNLTASDLVKDHLRYLI-------GGKLNVPDEVLCRFVFPERPGALMKF 560

Query: 567 LKDFQPNWNVSLFHHRHQVYGILTSDVLIGVQLEKSEEKKFDEYAKRVGYQYEVISQDDS 623
           L  F P WN+SLFH+R Q  G   ++VL+G+Q+ +SE  +F   A  +GY Y V++ D+ 
Sbjct: 561 LDSFSPRWNISLFHYRGQ--GETGANVLVGIQVPQSEMDEFCSRANSLGYDYVVVTDDND 612

BLAST of CmoCh03G011870 vs. TAIR10
Match: AT3G10050.1 (AT3G10050.1 L-O-methylthreonine resistant 1)

HSP 1 Score: 542.7 bits (1397), Expect = 2.9e-154
Identity = 285/536 (53.17%), Postives = 384/536 (71.64%), Query Frame = 1

Query: 89  VSWKELQYEEGSTG---HRQPLPADPDTEKQMEYITNILGSNVYDVAIESPLQLAPLLST 148
           VS   LQY  G  G    R     +    + MEY+TNIL + VYD+AIESPLQLA  LS 
Sbjct: 62  VSPNSLQYPAGYLGAVPERTNEAENGSIAEAMEYLTNILSTKVYDIAIESPLQLAKKLSK 121

Query: 149 QLGVNLWLKREDSQQVFSFKIRGAYNMISRLSEEKLKKGIICASAGNHAQGVALAGQRLK 208
           +LGV ++LKRED Q VFSFK+RGAYNM+ +L  ++L KG+IC+SAGNHAQGVAL+  +L 
Sbjct: 122 RLGVRMYLKREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQGVALSASKLG 181

Query: 209 TKAYIVMPTTTPQIKIDAVERLDGIVDLYGNSFDEAQKRAKERSEEEGLTFIPPFDDKDV 268
             A IVMP TTP+IK  AVE L   V L+G+S+D+AQ  AK R+EEEGLTFIPPFD  DV
Sbjct: 182 CTAVIVMPVTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTFIPPFDHPDV 241

Query: 269 IAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPDVKVFGVEPNDQNSMA 328
           IAGQGTVGMEI RQ +G +HAIFVP+GGGGL +GI ++ K V P+VK+ GVEP D N+MA
Sbjct: 242 IAGQGTVGMEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMA 301

Query: 329 QALYRGEIVNVTDIGHFADGVAVQQVGEETFRICYELLDDVILVKKESISAAIKDMFNDG 388
            +L+ GE V +  +G FADGVAV++VGEETFRI   L+D V+LV +++I A+IKDMF + 
Sbjct: 302 LSLHHGERVILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEK 361

Query: 389 RNILEPSGALSIAGAKAYCEYNNIKGVNIVAVTSGANMNFDQLGQISDLANI---DQSIL 448
           RNILEP+GAL++AGA+AYC+Y  +K VN+VA+TSGANMNFD+L  +++LAN+    +++L
Sbjct: 362 RNILEPAGALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVL 421

Query: 449 ATMLPETPGSLKQLTDLIADLGVTIAEMTYRFSSGSTDALVVYKVKAVDAA-LETLVRQL 508
           AT++PE PGS KQ  +L+  +   I+E  YR SS   +A+V+Y V    A  L+ L +++
Sbjct: 422 ATLMPEKPGSFKQFCELVGPM--NISEFKYRCSS-EKEAVVLYSVGVHTAGELKALQKRM 481

Query: 509 DFFGFKTYTLSDNEVVRNHLRYMVNMYTYIMGGRQNVENETLFRFTFPEKVGALKHFLKD 568
           +    KT  L+ +++V++HLR       Y+MGGR  V +E L RFTFPE+ GAL +FL  
Sbjct: 482 ESSQLKTVNLTTSDLVKDHLR-------YLMGGRSTVGDEVLCRFTFPERPGALMNFLDS 541

Query: 569 FQPNWNVSLFHHRHQVYGILTSDVLIGVQLEKSEEKKFDEYAKRVGYQYEVISQDD 618
           F P WN++LFH+R Q  G   ++VL+G+Q+ + E ++F   AK +GY Y ++S DD
Sbjct: 542 FSPRWNITLFHYRGQ--GETGANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDD 585

BLAST of CmoCh03G011870 vs. TAIR10
Match: AT4G11640.1 (AT4G11640.1 serine racemase)

HSP 1 Score: 154.8 bits (390), Expect = 1.7e-37
Identity = 97/297 (32.66%), Postives = 161/297 (54.21%), Query Frame = 1

Query: 135 SPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISRLSEEKLKKGIICASAGNHA 194
           +P+  +  L++  G +L+ K E  Q+  +FK RGA N +  L  E+  KG++  S+GNHA
Sbjct: 29  TPVLTSESLNSISGRSLFFKCECLQKGGAFKFRGACNAVLSLDAEQAAKGVVTHSSGNHA 88

Query: 195 QGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDLYGNSFDEAQKRAKERSEEEGL 254
             ++LA +     AYIV+P   P+ K+D V R  G V     +    ++ A +  +E G 
Sbjct: 89  AALSLAAKIQGIPAYIVVPKGAPKCKVDNVIRYGGKVIWSEATMSSREEIASKVLQETGS 148

Query: 255 TFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPDVKVF 314
             I P++D  +I+GQGT+ +E+  QI+ +I AI VPI GGGL SG+    K + P +++ 
Sbjct: 149 VLIHPYNDGRIISGQGTIALELLEQIQ-EIDAIVVPISGGGLISGVALAAKSIKPSIRII 208

Query: 315 GVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEETFRICYELLDDVILVKKESI 374
             EP   +  AQ+   G+I+ +      ADG+    +G+ T+ +  +L+DDV+ +++  I
Sbjct: 209 AAEPKGADDAAQSKVAGKIITLPVTNTIADGLRA-SLGDLTWPVVRDLVDDVVTLEECEI 268

Query: 375 SAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIK---GVNIVAVTSGANMNFDQL 429
             A+K  +   +  +EPSGA+ +A   +    NN       NI  V SG N++   L
Sbjct: 269 IEAMKMCYEILKVSVEPSGAIGLAAVLSNSFRNNPSCRDCKNIGIVLSGGNVDLGSL 323

BLAST of CmoCh03G011870 vs. TAIR10
Match: AT5G28020.1 (AT5G28020.1 cysteine synthase D2)

HSP 1 Score: 52.8 bits (125), Expect = 9.1e-07
Identity = 71/276 (25.72%), Postives = 110/276 (39.86%), Query Frame = 1

Query: 154 KREDSQQVFSFKIRGAYNMISRLSEEKL----KKGIICASAGNHAQGVALAGQRLKTKAY 213
           K E  +   S K R AY+MI    ++ L    K  +I  +AGN   G+A  G     K  
Sbjct: 37  KLEMMEPCSSVKDRIAYSMIKDAEDKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVI 96

Query: 214 IVMPTTTPQIKIDAVERLDGIVDLYGNSFD---EAQKRAKERSEEEGLTFIPPFDDK-DV 273
           +VMP+T    +   +  L   + L           +K     S+  G      F++  + 
Sbjct: 97  LVMPSTMSLERRIILRALGAELHLSDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANP 156

Query: 274 IAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIVSFYKLVYPDVKVFGVEPNDQNSMA 333
                T G EI R   GK+  +   +G GG A+G+  F K    D+KV  VEP +    +
Sbjct: 157 EIHYRTTGPEIWRDSAGKVDILVAGVGTGGTATGVGKFLKEQNKDIKVCVVEPVE----S 216

Query: 334 QALYRGEIVNVTDIGHFADGVAVQQVGEETFRICYELLDDVILVK-KESISAAIKDMFND 393
             L  G+        H   G+     G   F +   ++D++I V  +E+I  A      +
Sbjct: 217 PVLSGGQ-----PGPHLIQGIG---SGIVPFNLDLTIVDEIIQVAGEEAIETAKLLALKE 276

Query: 394 GRNILEPSGALSIAGAKAYCEYNNIKGVNIVAVTSG 421
           G  +   SGA + A  K      N   + +V   SG
Sbjct: 277 GLLVGISSGAAAAAALKVAKRPENAGKLIVVVFPSG 300

BLAST of CmoCh03G011870 vs. NCBI nr
Match: gi|449451080|ref|XP_004143290.1| (PREDICTED: threonine dehydratase biosynthetic, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 854.7 bits (2207), Expect = 9.8e-245
Identity = 458/631 (72.58%), Postives = 519/631 (82.25%), Query Frame = 1

Query: 1   MESILLTATTSNLPLPRNSSLSPLSFSSS-LPNQVSTKVDIRR-----TNKGTTKAPRIV 60
           MES LLT   +N  L RNSSLS LS SSS +  Q+++KV+I R      N  TT  P  +
Sbjct: 1   MESFLLT---TNPLLGRNSSLSSLSSSSSSISTQLNSKVEIIRRRIEKVNTRTTHVPASI 60

Query: 61  ASSSGSKGRAGKGVSANVAVDGSVAAVGAEAVKDVSWKELQYEEGSTGHRQPLPADPDTE 120
            +SS SK + GKG+SANVAVD SV  V  EAVKDVSW E+QYE GS GHR P PA+ D +
Sbjct: 61  VASSASKRKTGKGLSANVAVDCSV--VSEEAVKDVSWTEVQYESGSIGHRAPPPAEVDKD 120

Query: 121 KQMEYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMIS 180
           KQMEYIT ILGS VYDVA+E+PL+LAPLLSTQLGVNL+LKRED QQVFSFKIRGAYNMIS
Sbjct: 121 KQMEYITKILGSKVYDVAVETPLELAPLLSTQLGVNLYLKREDGQQVFSFKIRGAYNMIS 180

Query: 181 RLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDLY 240
           +LS++ LK G+ICASAGNHAQGVAL+ QRLK KA IVMPTTTP IK+DAV+RL G V   
Sbjct: 181 QLSKDDLKNGVICASAGNHAQGVALSAQRLKIKAQIVMPTTTPTIKVDAVKRLGGTVIQK 240

Query: 241 GNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGG 300
           G++FDE+QK AKE S   G+TFIPP+D++DVIAGQGTVGMEIGRQ+RGKIHAIFVPIGGG
Sbjct: 241 GDTFDESQKIAKEISCRCGITFIPPYDNEDVIAGQGTVGMEIGRQMRGKIHAIFVPIGGG 300

Query: 301 GLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEE 360
           GLA+GIVSFYKLVYP+VKVFGVEPND+NSMAQALYR EIVNVTDIGHFADGVAVQQVG E
Sbjct: 301 GLAAGIVSFYKLVYPEVKVFGVEPNDENSMAQALYRDEIVNVTDIGHFADGVAVQQVGNE 360

Query: 361 TFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVNI 420
            FRIC ELLDDVILV KE ISAAIKDMF+D R+ILEPSGAL+IA A+AYC+YNN+ GVN+
Sbjct: 361 NFRICRELLDDVILVTKEEISAAIKDMFSDERSILEPSGALAIAAARAYCKYNNVTGVNL 420

Query: 421 VAVTSGANMNFDQLGQISDLANIDQSILATMLPETPGSLKQLTDLIADLGVTIAEMTYRF 480
           VAV SGANMNFDQL +ISD+AN+DQSI+ TMLPETPGSLK+LT+L   LG  I EM YRF
Sbjct: 421 VAVCSGANMNFDQLREISDIANVDQSIICTMLPETPGSLKELTEL---LGENITEMQYRF 480

Query: 481 SSGSTDALVVYKVKAVDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYTYIMGG 540
           SSGS DA+VVYKV AV   LE +V  L+  GF TYTL+DN  V+NHLRYM        GG
Sbjct: 481 SSGSEDAVVVYKVSAVGKELEAMVELLNSAGFTTYTLNDNPTVKNHLRYMT-------GG 540

Query: 541 RQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGVQLEKS 600
           R ++ENETLFRFTFPE+ GALKHFLKDF+P WNVSLFHHR Q  GILTSDVLIGVQLEKS
Sbjct: 541 RADIENETLFRFTFPERKGALKHFLKDFKPTWNVSLFHHRSQ--GILTSDVLIGVQLEKS 600

Query: 601 EEKKFDEYAKRVGYQYEVISQDDSAHVLAKL 626
           E K F EY  +VGY+YEV+ QDD+AHVLAKL
Sbjct: 601 EHKHFHEYINKVGYKYEVVPQDDAAHVLAKL 614

BLAST of CmoCh03G011870 vs. NCBI nr
Match: gi|659125114|ref|XP_008462515.1| (PREDICTED: threonine dehydratase biosynthetic, chloroplastic-like [Cucumis melo])

HSP 1 Score: 852.0 bits (2200), Expect = 6.3e-244
Identity = 455/631 (72.11%), Postives = 522/631 (82.73%), Query Frame = 1

Query: 1   MESILLTATTSNLPLPRNSSLSPL-SFSSSLPNQVSTKVDIRR-----TNKGTTKAPRIV 60
           MES LLT   +N  L RNSSLS L S SSS+  Q+++KV+I R      N  TT  P  +
Sbjct: 1   MESFLLT---TNPLLGRNSSLSSLPSSSSSISTQLNSKVEIIRRRIEKVNTTTTHVPTSI 60

Query: 61  ASSSGSKGRAGKGVSANVAVDGSVAAVGAEAVKDVSWKELQYEEGSTGHRQPLPADPDTE 120
            +SS SK + GKG+SANVAVDGSVA VG   VKDVSW E+QYE GS GHR P PA+ D +
Sbjct: 61  VASSASKRKTGKGLSANVAVDGSVATVGT--VKDVSWTEVQYESGSIGHRAPPPAEVDKD 120

Query: 121 KQMEYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMIS 180
           KQMEYIT ILGS VYDVA+E+PL+LAPLLSTQ+G+NL+LKRED+QQVFSFKIRGAYNMIS
Sbjct: 121 KQMEYITKILGSKVYDVAVETPLELAPLLSTQIGINLYLKREDAQQVFSFKIRGAYNMIS 180

Query: 181 RLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDLY 240
           +L +++L  G+ICASAGNHAQGVAL+ QRLK KA IVMPTTTP IK++AV+RL G V   
Sbjct: 181 QLPKDQLANGVICASAGNHAQGVALSAQRLKIKAQIVMPTTTPAIKVEAVKRLGGKVIQE 240

Query: 241 GNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGG 300
           G +FDE+QK AKE S   G+TFIPPFD++DVIAGQGTVGMEIGRQ+RGKIHAI VPIGGG
Sbjct: 241 GETFDESQKIAKEISSRLGITFIPPFDNEDVIAGQGTVGMEIGRQMRGKIHAIIVPIGGG 300

Query: 301 GLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEE 360
           GLA+GIVSFYKLVYP+VKVFGVEPND+NSMAQALYR EIVNVTDIGHFADGVAVQQVG+E
Sbjct: 301 GLAAGIVSFYKLVYPEVKVFGVEPNDENSMAQALYRDEIVNVTDIGHFADGVAVQQVGDE 360

Query: 361 TFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVNI 420
           TFRIC ELLDDVILV KE ISAAIKDMF+D R+ILEPSGALSIA AKAYC+YNNI GVNI
Sbjct: 361 TFRICRELLDDVILVTKEEISAAIKDMFSDERSILEPSGALSIAAAKAYCKYNNITGVNI 420

Query: 421 VAVTSGANMNFDQLGQISDLANIDQSILATMLPETPGSLKQLTDLIADLGVTIAEMTYRF 480
           VAV SGANMNFDQL +ISD+AN+DQSI+ T+LPETPGSL+QLTD++ +    I EMTYRF
Sbjct: 421 VAVCSGANMNFDQLREISDIANVDQSIICTILPETPGSLQQLTDMLDE---DITEMTYRF 480

Query: 481 SSGSTDALVVYKVKAVDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYTYIMGG 540
           SSGS DA+VVYKV AV   LE +V  L+  GF TYTL+DN VV+NHLRYM        GG
Sbjct: 481 SSGSQDAVVVYKVSAVGTELEDMVEMLNTAGFTTYTLNDNPVVKNHLRYMT-------GG 540

Query: 541 RQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGVQLEKS 600
           R ++ENETLFRFTFPE+ GALKHF KDF+ +WNVSLFHHR Q  GILTSDVLIGVQLEKS
Sbjct: 541 RADLENETLFRFTFPERKGALKHFFKDFKQSWNVSLFHHRAQ--GILTSDVLIGVQLEKS 600

Query: 601 EEKKFDEYAKRVGYQYEVISQDDSAHVLAKL 626
           EEK F E+ ++VGY+YEV+ QDD+A VLAKL
Sbjct: 601 EEKDFHEHLRKVGYKYEVVPQDDAAQVLAKL 614

BLAST of CmoCh03G011870 vs. NCBI nr
Match: gi|449451239|ref|XP_004143369.1| (PREDICTED: threonine dehydratase biosynthetic, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 632.1 bits (1629), Expect = 1.0e-177
Identity = 348/636 (54.72%), Postives = 450/636 (70.75%), Query Frame = 1

Query: 1   MESILLTATTS-------NLPLPRNSSLSPLSFSSSLPNQVSTKVDIRRTNKGTTKAPRI 60
           MES+L T TTS          LP   S+ P S S  L N+  T   +RR+N  +  A   
Sbjct: 1   MESLLSTTTTSVPNSLALKSLLPSQPSIIPHS-SIQLSNRTKT---VRRSNFPSFLASLS 60

Query: 61  VASSSGSKGRAGKGVSANVAVDGSVAAVGAEAVKDVSWKELQYEEGSTGHRQPLPADPDT 120
                 +   +      N  VDGS+A V    V +VSWK+LQY  G  G     P   D 
Sbjct: 61  KLPKGSANSSSNNNTKTNAVVDGSIAIV--TGVPEVSWKDLQYPAGMLGAIPKRPEVIDE 120

Query: 121 EKQMEYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMI 180
            +QMEY+TNIL S VYDVAIESPL+LA  LSTQLG+ LWLKR+DSQ VFSFKIRGAYNM+
Sbjct: 121 RRQMEYLTNILSSKVYDVAIESPLELARKLSTQLGIQLWLKRDDSQFVFSFKIRGAYNMM 180

Query: 181 SRLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDL 240
           + L +E L++G+ICASAGNHAQGVALA  RL+T+A IVMP +TP IKI+AV  L G V L
Sbjct: 181 ANLPKEALERGVICASAGNHAQGVALAAGRLRTEAIIVMPRSTPPIKIEAVRSLGGNVLL 240

Query: 241 YGNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGG 300
           +G++FD+AQ+ A++ S+E  LT IPPFD++DVI GQGTVGMEIGRQ+RG +HAIFVP+GG
Sbjct: 241 HGDTFDDAQEHARQLSKERNLTIIPPFDNEDVIIGQGTVGMEIGRQMRGPLHAIFVPVGG 300

Query: 301 GGLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGE 360
           GGL +G+ SFYKLV+P+VK+ GVEPND NSMA AL+  ++V +  +G FADGVAV+QVG+
Sbjct: 301 GGLLAGVASFYKLVFPEVKIIGVEPNDANSMASALHNDQVVKLETVGTFADGVAVKQVGD 360

Query: 361 ETFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVN 420
           E FRI  EL+D ++LV K++I+A IKDMF D R+ILEP+GALSIAGAKAYCEYNNIKGVN
Sbjct: 361 ENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAGALSIAGAKAYCEYNNIKGVN 420

Query: 421 IVAVTSGANMNFDQLGQISDLA---NIDQSILATMLPETPGSLKQLTDLIADLGVTIAEM 480
           IVAVTSGANMNFDQLG I+D A   N  ++  AT+LPE PGSL   +DL+    VT  E 
Sbjct: 421 IVAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKPGSLITFSDLMGSRSVT--EF 480

Query: 481 TYRFSSGSTDALVVYKVKA-VDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYT 540
            YRF+S   +A+V+Y V   V + L  + ++++   F+TY L+ NE+V++HLRYM     
Sbjct: 481 KYRFNS-EKNAIVLYSVGVKVASELGEVKKKIESSPFETYDLTKNELVKDHLRYM----- 540

Query: 541 YIMGGRQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGV 600
             MGGR +V NE  +RFT PE+ GAL  FL  F P WN+SL H+R Q  GI+++DVL+G+
Sbjct: 541 --MGGRSSVPNEVFYRFTLPERSGALLQFLDAFSPRWNISLIHYRRQ--GIISADVLVGL 600

Query: 601 QLEKSEEKKFDEYAKRVGYQYEVISQDDSAHVLAKL 626
           Q+E SEE +F E A+++G+ Y V+S D ++ +L ++
Sbjct: 601 QIEGSEEAEFHESARKLGFGYVVVSNDPASKLLTQV 618

BLAST of CmoCh03G011870 vs. NCBI nr
Match: gi|449451082|ref|XP_004143291.1| (PREDICTED: threonine dehydratase biosynthetic, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 568.2 bits (1463), Expect = 1.8e-158
Identity = 317/627 (50.56%), Postives = 432/627 (68.90%), Query Frame = 1

Query: 1   MESILLTATTSNLPLPRNSSL-SPLSFSSSLPNQVSTKVDIRRTNKGTTKAPRIVASSSG 60
           M+++LL A TS+ P+PR S L SP SF     N  S  +D  R     T+ P I+A+ S 
Sbjct: 1   MDALLLNAPTSH-PIPRKSFLPSPPSFF----NGSSISIDDNRNRVRLTRPPIILATLSK 60

Query: 61  SKGRAGKGVSANVAVDGSVAAVGAEAV---KDVSWKELQYEEGSTG---HRQPLPADPDT 120
                     +N +V     A+    +   K VS   LQ+  G  G    R       D+
Sbjct: 61  HALDIPPNSVSNTSVPTPADALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDS 120

Query: 121 EKQMEYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMI 180
              MEY+T ILGS VYDVAIESPLQLAP LS + GVN+WLKRED Q VFSFK+RGAYNM+
Sbjct: 121 VDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMM 180

Query: 181 SRLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDL 240
           ++L +E+L+KG+IC+SAGNHAQGVALA +RL+  A I MP TTP+IK  +V++L   V L
Sbjct: 181 AKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVL 240

Query: 241 YGNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGG 300
            G+S+DEAQ  AK+RS EEG TFIPPFD  DVIAGQGTVGMEI RQI+G +HAIFVP+GG
Sbjct: 241 VGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGG 300

Query: 301 GGLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGE 360
           GGL +GI ++ K V P+VK+ GVEP+D N+MA +L  G+ V +   G FADGVAV++VGE
Sbjct: 301 GGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGE 360

Query: 361 ETFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVN 420
           ETFR+C +L+D V+LV +++I A+IKDMF + R+ILEP+GALS+AGA+AYC+Y  +KG N
Sbjct: 361 ETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGEN 420

Query: 421 IVAVTSGANMNFDQLGQISDLANI---DQSILATMLPETPGSLKQLTDLIADLGVTIAEM 480
           +V +TSGANMNFD+L  +++LAN+    +++LAT+LPETPGS K+  +L+  + +T  E 
Sbjct: 421 VVVITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNIT--EF 480

Query: 481 TYRFSSGSTDALVVYKVKA-VDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYT 540
            YR+SS   +A+V+Y V   + + LE +  +++     TY L+ N++V++HLR       
Sbjct: 481 KYRYSS-EKEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLR------- 540

Query: 541 YIMGGRQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGV 600
           Y+MGG+ NVENE L RF FPE+ GAL+ FL    P WN++LFH  +Q  G   +++L+G+
Sbjct: 541 YLMGGKSNVENEVLCRFIFPERPGALEKFLDALSPRWNITLFH--YQSKGETGANILVGL 600

Query: 601 QLEKSEEKKFDEYAKRVGYQYEVISQD 617
           Q+EK +  +F E A+RVGY Y V++ D
Sbjct: 601 QIEKPDMGEFHERARRVGYDYVVVTDD 610

BLAST of CmoCh03G011870 vs. NCBI nr
Match: gi|659125116|ref|XP_008462516.1| (PREDICTED: threonine dehydratase biosynthetic, chloroplastic [Cucumis melo])

HSP 1 Score: 567.0 bits (1460), Expect = 4.1e-158
Identity = 316/627 (50.40%), Postives = 431/627 (68.74%), Query Frame = 1

Query: 1   MESILLTATTSNLPLPRNSSL-SPLSFSSSLPNQVSTKVDIRRTNKGTTKAPRIVASSSG 60
           ME++LL A TS+ P+PR S L SP SF     N  S  +D  R     T+ P I A+ S 
Sbjct: 1   MEALLLNAPTSH-PIPRKSFLPSPPSFF----NGTSISIDDNRNRVRLTRPPFIQATLSK 60

Query: 61  SKGRAGKGVSANVAVD---GSVAAVGAEAVKDVSWKELQYEEGSTG---HRQPLPADPDT 120
                     +N ++     ++A       + VS   LQ+  G  G    R       D+
Sbjct: 61  HAVDIPPNSVSNTSIPTPGDALAPTPLPPRRRVSADSLQFPPGYLGAVPDRSGSDNGEDS 120

Query: 121 EKQMEYITNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMI 180
              MEY+T ILGS VYDVAIESPLQLAP LS +LG N+WLKRED Q VFSFK+RGAYNM+
Sbjct: 121 VDAMEYLTRILGSKVYDVAIESPLQLAPTLSERLGNNIWLKREDLQPVFSFKLRGAYNMM 180

Query: 181 SRLSEEKLKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDL 240
           ++L +E+L+KG+IC+SAGNHAQGVALA +RL+  A I MP TTP+IK  +V++L   V L
Sbjct: 181 AKLPQEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVL 240

Query: 241 YGNSFDEAQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGG 300
            G+S+DEAQ  AK+RS EEG TFIPPFD  DVIAGQGTVGMEI RQI+G +HAIFVP+GG
Sbjct: 241 VGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGG 300

Query: 301 GGLASGIVSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGE 360
           GGL +GI ++ K V P+VK+ GVEP+D N+MA +L  G+ V +   G FADGVAV++VGE
Sbjct: 301 GGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGE 360

Query: 361 ETFRICYELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVN 420
           ETFR+C +L+D V+LV +++I A+IKDMF + R+ILEP+GALS+AGA+AYC+Y  +KG N
Sbjct: 361 ETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGEN 420

Query: 421 IVAVTSGANMNFDQLGQISDLANI---DQSILATMLPETPGSLKQLTDLIADLGVTIAEM 480
           +V +TSGANMNFD+L  +++LAN+    +++LAT+LPETPGS K+  +L+  + +T  E 
Sbjct: 421 VVVITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNIT--EF 480

Query: 481 TYRFSSGSTDALVVYKVKA-VDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMVNMYT 540
            YR+SS   +A+V+Y V   + + LE +  +++     TY L+ N++V++HLR       
Sbjct: 481 KYRYSS-EKEAVVLYSVGIHMPSELEEMKNRMESSQLPTYNLTKNDLVKDHLR------- 540

Query: 541 YIMGGRQNVENETLFRFTFPEKVGALKHFLKDFQPNWNVSLFHHRHQVYGILTSDVLIGV 600
           Y+MGG+ NVENE L RF FPE+ GAL+ FL    P WN++LFH  +Q  G   +++L+G+
Sbjct: 541 YLMGGKSNVENEVLCRFIFPERPGALEKFLDALSPRWNITLFH--YQSKGETGANILVGL 600

Query: 601 QLEKSEEKKFDEYAKRVGYQYEVISQD 617
           Q+EK +  +F E A+RVGY Y V++ D
Sbjct: 601 QIEKPDMGEFHERARRVGYDYVVVTDD 610

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
THD1_ARATH5.2e-15353.17Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana GN=OMR... [more]
THD1_SOLLC3.6e-13849.52Threonine dehydratase biosynthetic, chloroplastic OS=Solanum lycopersicum GN=TD ... [more]
ILVA_BURM11.4e-11846.00L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain AT... [more]
ILVA_HAEIN3.4e-11243.31L-threonine dehydratase biosynthetic IlvA OS=Haemophilus influenzae (strain ATCC... [more]
ILVA_PASMU9.2e-11043.54L-threonine dehydratase biosynthetic IlvA OS=Pasteurella multocida (strain Pm70)... [more]
Match NameE-valueIdentityDescription
A0A0A0KKC3_CUCSA6.8e-24572.58Threonine dehydratase OS=Cucumis sativus GN=Csa_6G448740 PE=3 SV=1[more]
A0A0A0KHC3_CUCSA7.2e-17854.72Threonine dehydratase OS=Cucumis sativus GN=Csa_6G448720 PE=3 SV=1[more]
A0A0A0KII4_CUCSA1.3e-15850.56Threonine dehydratase OS=Cucumis sativus GN=Csa_6G449240 PE=3 SV=1[more]
V7BJQ0_PHAVU1.7e-15552.95Threonine dehydratase OS=Phaseolus vulgaris GN=PHAVU_007G225400g PE=3 SV=1[more]
U5G6J7_POPTR1.9e-15452.94Threonine dehydratase OS=Populus trichocarpa GN=POPTR_0006s11980g PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10050.12.9e-15453.17 L-O-methylthreonine resistant 1[more]
AT4G11640.11.7e-3732.66 serine racemase[more]
AT5G28020.19.1e-0725.72 cysteine synthase D2[more]
Match NameE-valueIdentityDescription
gi|449451080|ref|XP_004143290.1|9.8e-24572.58PREDICTED: threonine dehydratase biosynthetic, chloroplastic-like [Cucumis sativ... [more]
gi|659125114|ref|XP_008462515.1|6.3e-24472.11PREDICTED: threonine dehydratase biosynthetic, chloroplastic-like [Cucumis melo][more]
gi|449451239|ref|XP_004143369.1|1.0e-17754.72PREDICTED: threonine dehydratase biosynthetic, chloroplastic-like [Cucumis sativ... [more]
gi|449451082|ref|XP_004143291.1|1.8e-15850.56PREDICTED: threonine dehydratase biosynthetic, chloroplastic-like [Cucumis sativ... [more]
gi|659125116|ref|XP_008462516.1|4.1e-15850.40PREDICTED: threonine dehydratase biosynthetic, chloroplastic [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000634Ser/Thr_deHydtase_PyrdxlP-BS
IPR001721TD_ACT-like
IPR001926PLP-dep
IPR005787Thr_deHydtase_biosynth
Vocabulary: Biological Process
TermDefinition
GO:0006520cellular amino acid metabolic process
GO:0009097isoleucine biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0030170pyridoxal phosphate binding
GO:0004794L-threonine ammonia-lyase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006544 glycine metabolic process
biological_process GO:0009097 isoleucine biosynthetic process
biological_process GO:0006563 L-serine metabolic process
biological_process GO:0006566 threonine metabolic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004794 L-threonine ammonia-lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G011870.1CmoCh03G011870.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 156..169
scor
IPR001721ACT-like domainPFAMPF00585Thr_dehydrat_Ccoord: 438..521
score: 3.2E-8coord: 536..619
score: 2.5
IPR001721ACT-like domainPROFILEPS51672ACT_LIKEcoord: 542..615
score: 9.479coord: 440..513
score: 12
IPR001926Tryptophan synthase beta subunit-like PLP-dependent enzymePFAMPF00291PALPcoord: 132..420
score: 8.7
IPR001926Tryptophan synthase beta subunit-like PLP-dependent enzymeunknownSSF53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 120..466
score: 1.96
IPR005787Threonine dehydratase, biosyntheticTIGRFAMsTIGR01124TIGR01124coord: 118..617
score: 6.3E
NoneNo IPR availableGENE3DG3DSA:3.40.1020.10coord: 467..618
score: 9.7
NoneNo IPR availableGENE3DG3DSA:3.40.50.1100coord: 183..431
score: 5.7
NoneNo IPR availablePANTHERPTHR10314SER/THR DEHYDRATASE, TRP SYNTHASEcoord: 90..469
score: 4.1E
NoneNo IPR availablePANTHERPTHR10314:SF30THREONINE DEHYDRATASE, MITOCHONDRIALcoord: 90..469
score: 4.1E
NoneNo IPR availableunknownSSF55021ACT-likecoord: 535..620
score: 2.4E-13coord: 440..525
score: 1.05

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None