CmaCh20G005370 (gene) Cucurbita maxima (Rimu)

NameCmaCh20G005370
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionLysosomal Pro-X carboxypeptidase, putative
LocationCma_Chr20 : 2587392 .. 2591016 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTTTCCCATGTTTTCATCCCCATGGCTTCCTTTTATACTTCTCATCCTCTCAACCTGTGTCACTGCAACACAGTATAGAATTCCAAGGCTAAGTCCATTTAGCAGAACCTTTCTTCCTAACACTAAAGCTTCACCTGTTTCCGATGATTTCAAGACATTTTATTACAACCAAACGTTGGATCACTTCAACTACAAGCCTGAGAGCTACACATCCTTCCCTCATAGATATATAATCAACTTTAATTATTGGGGCGGCGCAAATTCTAGTGCTCCAATTCTTGCCTACTTGGGCGCTGAAGGGCCACTGGAAGGAGATTTGAACGCTATAGGGTTCATGACTGATAATGCTCTTCAATTTGATGCTCTTCTTGTTTATATTGAGGTATGGAATTACATTTACTTTAGAACTTTTATGTAATTTTCTTATGGTCTAACACTTGTTGTGTTCTTGTTAGCATCGATATTATGGGAAATCAATACCTTTTGGATCAAGGCAAGAAGCACTTAAGAACGCTAGCACTCTAGGCTATTTCAACTCGGCTCAAGCGATAGCAGATTATGCAGCTGTTCTTATACATTTGAAAAAGAAGTTACATGCTAAAGATTGTCCTGTAATTGTCCTTGGTGGGTCATATGGAGGAAGTAAGTAACTTCTCATTTAAAATCTTTAAACTCTATTGCATTGGCATGTAATATAACGTAAATTATTATGTACTCAGTGTTGGCTGCATGGTTCCGGCTGAAATATCCTAATGTTGCACTTGGAGCTTTGGCGTCTTCGGCCCCGATTCTTTACTTCGACGATATCACGCCACATAATGGATACTATTCTATTGCCACCAAGGATTTTAAAGTAAGATCCTAGAATCATTGATGATCTATTTCAACTTTTAATGTTTTTGTAATGGTCCAGGCTCACGACTAACAGATATTGTCCTCTTTGGGTTTTCCCTTATGAACTTTTCCTCACATTCTGTTAGGGGAAGGTTTTCATGCCCTTATAAAGGGTATTTCGTTCTCCTCCTCAACCAAGGTGGGATATCACAGATTTTAAAAGCAAAAATGTTAATGAATTTGCAGGAAGTTAGTGAGAGTTGCTATGAAACTATTCGAGATTCGTGGTCTGAAATGGAAGCTATGGCTTCTAAGCCTAATGGCCTTTCCATCCTTAGCAAGGAGTTCAAAACATGCAGGTATTAGAATGGCTTTCCATATCTGAATTTGATGAAGTTGAAGAAAGTTGTAGTTTTCATATCCATTTGGTTTGTTTTGTGTTTTGTTGTTGTAGTCCTCTGAATAGTTACTCTCAGCTGGAAGACTACTTGTGGTCAATGTATGCGGGTGCAGCCCAATACAACCAACCACCAAGATATCCGGTCACTAGAATCTGTGGTGGTATTGATGGAGCTTCTTCTGAAAGTGGAACTCTTGGCAAAATAGCTGCAGGTGTTTTTGCTTATGAAGGAAAGCTATCCTGCTACAATCTTGAGCCCAAAAATGAAACTGAAACCGATGTAGGTTGGAGATGGCAGGTAAATCTTTTATTGAAGACATCAATATATGAGTAATATGATTACTAATGTGACCGTCCAACCCTACCACTAGTAGATATTGTCCTTTTTGGGCTTTCCCCTCGAGTTTTTAAAACGCGTCTGCTAGAAAGAGGTTTCCACACCCTTAGAAAGAACGTTTCGTTCTCCTCCCCAACCATGTGGGATCTCACAATTCACCCCCTTCAAGACCCAGCATCCTTGCTGGCGCTCGTTCCCCTTTCCATCGATGTCGTTTCCCTCTCCAATCGATGTGGGATCTCACAATCCACCTCCTTCGGGGCACAACGTTTTCACTAATACACCGCCTGTTGTCCACCCCCTTCAGGGCTAAGTGTCTTCATTGGCACACCACCCGGTTGTTGGATGAAAGTCCCACATCGGCTAATTTAGGGAATGATCATGGTTTTATAATCAAGGAATACTATCTCTATTGACATGAGGCCTTTTGGAGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTTAAAGTGGACAATATCATTCCATTGTGGAGATCCGTGATTCCAAACATCAGTAACCTGGCTATTATACTATTTGTAACAGACTAAACCCACCACTAGTAGATATTGTCCTCTTTGGGCTTTCGCTTTCAACGTGTCTACTAGAGAGAAGTTTCCACACCCTGAATGCTATCTAATTCACATATTTGTTATATTCCCAAAACAAAGGAAGAAGTTTAATAGGATTTTTCACTTTTTATTTGTACATCTATGAATGCTGGTGAAATTTGTTCAATCTTAGTAGTGAAGATTATGATTATTTCTACACTGAAGTAGCTTCTGTATACTCCAGAAATGCAGTGAGATGGTGATGCCAATAGGCACAGACAATGATACTATGTTTCCACCATACACTTTTGACCTTAGAAGCTTCATAAATCACTGCAATCAGTTATACGGCGTCTCTCCCAGGCCTCACTGGGTCACCACCTATTATGGTGGCAATGTATGCACTCAACCATTTCTCTCTTTCATTTTTTAAATTATCCACTTTCTCCCAAGTTTTTGGTAACTTTTGAGCCTTCTTTAACCAAATTTCAAAAACAAAATTAAGTTAAAAAAAAACTAAGTAGCCCAGGTAGAAGTAGTTTTATAAGCTTCATTTTAGAAAATAGAAAACTAAAAAACAAAATATTTCGATTTCTCCATTCTTTCTTATCTCCATTCAAATTACAGGACATAAAACTCCACCTTCAAAGATTTGGCAGCAACATCATTTTCTCCAATGGACTCAGAGATCCTTATAGCAGTGGCGGGTAAGAGGATTTTGAAATTGTTAGCTCCCACATTCGTTGGAGAGGGGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAATATACGCGTTCTAAAACTTTGAGGGAAACCCCAGAAGGAAAAGCCCATAGAAGATAATATCTGCTAGCGGTGGACTTAAGCTATTACAGAAATGGTTTAGATCAATCATCCCTCTCTCCCCTTTCTTTGTGAATACAGAGTATTGCAAAGCTTATCAGACAGTCTCCTTGCAGTTCATACAGCCAACGGTAAAAGTCTTCTCTTTTTCCTTCCCTTCTTTGATTCATAGTCATAAAATTCTAACTACAAATGTTTGTTTTTCAGGGTCCCATTGTTTGGACATTTTACGGGCAAATGAAACCGATCCACAATGGTTAGTGAAACAAAGAGAAACAGAAGTTAGCATCATTAAAGAATGGATCAGGAAGTACTATGCCGATCTTAAGGAGTTCAAACAATAGCTAAAAAGAACACAAAAGGGTGATGAAGGGGCACCCTACTTCGAACATAAATTTATGACCTTGTATTGAATCCATCAAAGAATGTGGTGAAAAGCCCTTTTCAGAGTTATGAAAGACTCTTAATAAATGGTGGTGCCCTTTCTTTTCTTCCCTACTTGGAACAGCATAAGAAAATGATTGTGTAGAAGATAGTGTTGAAAGTGTCCTTTACTTGCCACTTGAAGAAACATGACCACAAGCAAGTCAACTATTCCACTATTTTTAATGGGTGTTTCTTGTTGGAGGATGACTCATGAAACCAAA

mRNA sequence

ATGAATTTTCCCATGTTTTCATCCCCATGGCTTCCTTTTATACTTCTCATCCTCTCAACCTGTGTCACTGCAACACAGTATAGAATTCCAAGGCTAAGTCCATTTAGCAGAACCTTTCTTCCTAACACTAAAGCTTCACCTGTTTCCGATGATTTCAAGACATTTTATTACAACCAAACGTTGGATCACTTCAACTACAAGCCTGAGAGCTACACATCCTTCCCTCATAGATATATAATCAACTTTAATTATTGGGGCGGCGCAAATTCTAGTGCTCCAATTCTTGCCTACTTGGGCGCTGAAGGGCCACTGGAAGGAGATTTGAACGCTATAGGGTTCATGACTGATAATGCTCTTCAATTTGATGCTCTTCTTGTTTATATTGAGCATCGATATTATGGGAAATCAATACCTTTTGGATCAAGGCAAGAAGCACTTAAGAACGCTAGCACTCTAGGCTATTTCAACTCGGCTCAAGCGATAGCAGATTATGCAGCTGTTCTTATACATTTGAAAAAGAAGTTACATGCTAAAGATTGTCCTGTAATTGTCCTTGGTGGGTCATATGGAGGAATGTTGGCTGCATGGTTCCGGCTGAAATATCCTAATGTTGCACTTGGAGCTTTGGCGTCTTCGGCCCCGATTCTTTACTTCGACGATATCACGCCACATAATGGATACTATTCTATTGCCACCAAGGATTTTAAAGAAGTTAGTGAGAGTTGCTATGAAACTATTCGAGATTCGTGGTCTGAAATGGAAGCTATGGCTTCTAAGCCTAATGGCCTTTCCATCCTTAGCAAGGAGTTCAAAACATGCAGTCCTCTGAATAGTTACTCTCAGCTGGAAGACTACTTGTGGTCAATGTATGCGGGTGCAGCCCAATACAACCAACCACCAAGATATCCGGTCACTAGAATCTGTGGTGGTATTGATGGAGCTTCTTCTGAAAGTGGAACTCTTGGCAAAATAGCTGCAGGTGTTTTTGCTTATGAAGGAAAGCTATCCTGCTACAATCTTGAGCCCAAAAATGAAACTGAAACCGATGTAGGTTGGAGATGGCAGAAATGCAGTGAGATGGTGATGCCAATAGGCACAGACAATGATACTATGTTTCCACCATACACTTTTGACCTTAGAAGCTTCATAAATCACTGCAATCAGTTATACGGCGTCTCTCCCAGGCCTCACTGGGTCACCACCTATTATGGTGGCAATGACATAAAACTCCACCTTCAAAGATTTGGCAGCAACATCATTTTCTCCAATGGACTCAGAGATCCTTATAGCAGTGGCGGAGTATTGCAAAGCTTATCAGACAGTCTCCTTGCAGTTCATACAGCCAACGGGTCCCATTGTTTGGACATTTTACGGGCAAATGAAACCGATCCACAATGGTTAGTGAAACAAAGAGAAACAGAAGTTAGCATCATTAAAGAATGGATCAGGAAGTACTATGCCGATCTTAAGGAGTTCAAACAATAGCTAAAAAGAACACAAAAGGGTGATGAAGGGGCACCCTACTTCGAACATAAATTTATGACCTTGTATTGAATCCATCAAAGAATGTGGTGAAAAGCCCTTTTCAGAGTTATGAAAGACTCTTAATAAATGGTGGTGCCCTTTCTTTTCTTCCCTACTTGGAACAGCATAAGAAAATGATTGTGTAGAAGATAGTGTTGAAAGTGTCCTTTACTTGCCACTTGAAGAAACATGACCACAAGCAAGTCAACTATTCCACTATTTTTAATGGGTGTTTCTTGTTGGAGGATGACTCATGAAACCAAA

Coding sequence (CDS)

ATGAATTTTCCCATGTTTTCATCCCCATGGCTTCCTTTTATACTTCTCATCCTCTCAACCTGTGTCACTGCAACACAGTATAGAATTCCAAGGCTAAGTCCATTTAGCAGAACCTTTCTTCCTAACACTAAAGCTTCACCTGTTTCCGATGATTTCAAGACATTTTATTACAACCAAACGTTGGATCACTTCAACTACAAGCCTGAGAGCTACACATCCTTCCCTCATAGATATATAATCAACTTTAATTATTGGGGCGGCGCAAATTCTAGTGCTCCAATTCTTGCCTACTTGGGCGCTGAAGGGCCACTGGAAGGAGATTTGAACGCTATAGGGTTCATGACTGATAATGCTCTTCAATTTGATGCTCTTCTTGTTTATATTGAGCATCGATATTATGGGAAATCAATACCTTTTGGATCAAGGCAAGAAGCACTTAAGAACGCTAGCACTCTAGGCTATTTCAACTCGGCTCAAGCGATAGCAGATTATGCAGCTGTTCTTATACATTTGAAAAAGAAGTTACATGCTAAAGATTGTCCTGTAATTGTCCTTGGTGGGTCATATGGAGGAATGTTGGCTGCATGGTTCCGGCTGAAATATCCTAATGTTGCACTTGGAGCTTTGGCGTCTTCGGCCCCGATTCTTTACTTCGACGATATCACGCCACATAATGGATACTATTCTATTGCCACCAAGGATTTTAAAGAAGTTAGTGAGAGTTGCTATGAAACTATTCGAGATTCGTGGTCTGAAATGGAAGCTATGGCTTCTAAGCCTAATGGCCTTTCCATCCTTAGCAAGGAGTTCAAAACATGCAGTCCTCTGAATAGTTACTCTCAGCTGGAAGACTACTTGTGGTCAATGTATGCGGGTGCAGCCCAATACAACCAACCACCAAGATATCCGGTCACTAGAATCTGTGGTGGTATTGATGGAGCTTCTTCTGAAAGTGGAACTCTTGGCAAAATAGCTGCAGGTGTTTTTGCTTATGAAGGAAAGCTATCCTGCTACAATCTTGAGCCCAAAAATGAAACTGAAACCGATGTAGGTTGGAGATGGCAGAAATGCAGTGAGATGGTGATGCCAATAGGCACAGACAATGATACTATGTTTCCACCATACACTTTTGACCTTAGAAGCTTCATAAATCACTGCAATCAGTTATACGGCGTCTCTCCCAGGCCTCACTGGGTCACCACCTATTATGGTGGCAATGACATAAAACTCCACCTTCAAAGATTTGGCAGCAACATCATTTTCTCCAATGGACTCAGAGATCCTTATAGCAGTGGCGGAGTATTGCAAAGCTTATCAGACAGTCTCCTTGCAGTTCATACAGCCAACGGGTCCCATTGTTTGGACATTTTACGGGCAAATGAAACCGATCCACAATGGTTAGTGAAACAAAGAGAAACAGAAGTTAGCATCATTAAAGAATGGATCAGGAAGTACTATGCCGATCTTAAGGAGTTCAAACAATAG

Protein sequence

MNFPMFSSPWLPFILLILSTCVTATQYRIPRLSPFSRTFLPNTKASPVSDDFKTFYYNQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDNALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHAKDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKEVSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYNQPPRYPVTRICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQKCSEMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGSNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVSIIKEWIRKYYADLKEFKQ
BLAST of CmaCh20G005370 vs. Swiss-Prot
Match: PCP_MOUSE (Lysosomal Pro-X carboxypeptidase OS=Mus musculus GN=Prcp PE=1 SV=2)

HSP 1 Score: 319.7 bits (818), Expect = 5.6e-86
Identity = 184/503 (36.58%), Postives = 280/503 (55.67%), Query Frame = 1

Query: 11  LPFILLILSTCVTATQYRIPRLSPFSRTFLPNTKASPVSDD-----FKTFYYNQTLDHFN 70
           L F+LL  +T +       PRL        P+  ASP  D      +   Y+ Q +DHF 
Sbjct: 9   LSFLLLGAATTIP------PRLKTLGS---PHLSASPTPDPAVARKYSVLYFEQKVDHFG 68

Query: 71  YKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDNALQFDALL 130
           +      +F  RY++   +W    +   IL Y G EG +    N  GFM D A +  A+L
Sbjct: 69  FA--DMRTFKQRYLVADKHW--QRNGGSILFYTGNEGDIVWFCNNTGFMWDVAEELKAML 128

Query: 131 VYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLH-AKDCPVIV 190
           V+ EHRYYG+S+PFG  Q++ K++  L +  S QA+AD+A ++ HL+K +  A+  PVI 
Sbjct: 129 VFAEHRYYGESLPFG--QDSFKDSQHLNFLTSEQALADFAELIRHLEKTIPGAQGQPVIA 188

Query: 191 LGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKEVSESCYE 250
           +GGSYGGMLAAWFR+KYP++ +GALA+SAPI   D + P   +  I T DF++    C E
Sbjct: 189 IGGSYGGMLAAWFRMKYPHIVVGALAASAPIWQLDGMVPCGEFMKIVTNDFRKSGPYCSE 248

Query: 251 TIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNS--YSQLEDYLWSMYAGAAQYNQP--- 310
           +IR SW+ ++ ++   +GL  L+     CSPL S     L+ ++   +   A  N P   
Sbjct: 249 SIRKSWNVIDKLSGSGSGLQSLTNILHLCSPLTSEKIPTLKGWIAETWVNLAMVNYPYAC 308

Query: 311 ------PRYPVTRICGGIDGAS-SESGTLGKIAAGV---FAYEGKLSCYNLEPKNETET- 370
                 P +P+  +C  +   + S++  L  I   +   + Y G+ +C N+     +   
Sbjct: 309 NFLQPLPAWPIKEVCQYLKNPNVSDTVLLQNIFQALSVYYNYSGQAACLNISQTTTSSLG 368

Query: 371 DVGWRWQKCSEMVMPIGTDN-DTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGND 430
            +GW +Q C+EMVMP  T+  D MF P+ +DL  + N C   +GV PRPHW+TT YGG +
Sbjct: 369 SMGWSFQACTEMVMPFCTNGIDDMFEPFLWDLEKYSNDCFNQWGVKPRPHWMTTMYGGKN 428

Query: 431 IKLHLQRFGSNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWL 490
           I  H     SNIIFSNG  DP+S GGV + ++D+L+A++  +G+H LD+   N  DP  +
Sbjct: 429 ISSH-----SNIIFSNGELDPWSGGGVTRDITDTLVAINIHDGAHHLDLRAHNAFDPSSV 488

BLAST of CmaCh20G005370 vs. Swiss-Prot
Match: PCP_PONAB (Lysosomal Pro-X carboxypeptidase OS=Pongo abelii GN=PRCP PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 4.7e-85
Identity = 182/494 (36.84%), Postives = 277/494 (56.07%), Query Frame = 1

Query: 14  ILLILSTCVTATQYRI-PRLSPFSRTFLPNTKAS--PVSDDFKTFYYNQTLDHFNYKPES 73
           +LL+LS     T   + P L       LP    S   V+ ++   Y+ Q +DHF +   +
Sbjct: 7   LLLLLSFLAPWTTIALRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFN--T 66

Query: 74  YTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDNALQFDALLVYIEH 133
             +F  RY++   YW    +   IL Y G EG +    N  GFM D A +  A+LV+ EH
Sbjct: 67  VKTFNQRYLVADKYW--KKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEH 126

Query: 134 RYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLH-AKDCPVIVLGGSY 193
           RYYG+S+PFG      K++  L +  S QA+AD+A ++ HLK+ +  A++ PVI +GGSY
Sbjct: 127 RYYGESLPFGDN--TFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSY 186

Query: 194 GGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKEVSESCYETIRDS 253
           GGMLAAWFR+KYP++ +GALA+SAPI  F+D+ P   +  I T DF++    C E+IR S
Sbjct: 187 GGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIRRS 246

Query: 254 WSEMEAMASKPNGLSILSKEFKTCSPLNS--YSQLEDYLWSMYAGAAQYNQP-------- 313
           W  +  +++  +GL  L+     CSPL S     L+D++   +   A  + P        
Sbjct: 247 WDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQP 306

Query: 314 -PRYPVTRICGGIDGAS-SESGTLGKIAAGV---FAYEGKLSCYNL-EPKNETETDVGWR 373
            P +P+  +C  +   + S+S  L  I   +   + Y G++ C N+ E    +   +GW 
Sbjct: 307 LPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWS 366

Query: 374 WQKCSEMVMPIGTDN-DTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHL 433
           +Q C+E+VMP  T+  D MF P++++L+   + C Q +GV PRP W+TT YGG +I  H 
Sbjct: 367 YQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSH- 426

Query: 434 QRFGSNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRE 487
               +NI+FSNG  DP+S GGV + ++D+L+AV  + G+H LD+   N  DP  ++  R 
Sbjct: 427 ----TNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPTSVLLARS 486

BLAST of CmaCh20G005370 vs. Swiss-Prot
Match: PCP_HUMAN (Lysosomal Pro-X carboxypeptidase OS=Homo sapiens GN=PRCP PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.8e-84
Identity = 182/494 (36.84%), Postives = 277/494 (56.07%), Query Frame = 1

Query: 14  ILLILSTCVT-ATQYRIPRLSPFSRTFLPNTKAS--PVSDDFKTFYYNQTLDHFNYKPES 73
           +LL+LS     AT    P L       LP    S   V+ ++   Y+ Q +DHF +   +
Sbjct: 7   LLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFN--T 66

Query: 74  YTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDNALQFDALLVYIEH 133
             +F  RY++   YW    +   IL Y G EG +    N  GFM D A +  A+LV+ EH
Sbjct: 67  VKTFNQRYLVADKYW--KKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEH 126

Query: 134 RYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLH-AKDCPVIVLGGSY 193
           RYYG+S+PFG    + K++  L +  S QA+AD+A ++ HLK+ +  A++ PVI +GGSY
Sbjct: 127 RYYGESLPFGDN--SFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSY 186

Query: 194 GGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKEVSESCYETIRDS 253
           GGMLAAWFR+KYP++ +GALA+SAPI  F+D+ P   +  I T DF++    C E+I  S
Sbjct: 187 GGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRS 246

Query: 254 WSEMEAMASKPNGLSILSKEFKTCSPLNS--YSQLEDYLWSMYAGAAQYNQP-------- 313
           W  +  +++  +GL  L+     CSPL S     L+D++   +   A  + P        
Sbjct: 247 WDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQP 306

Query: 314 -PRYPVTRICGGIDGAS-SESGTLGKIAAGV---FAYEGKLSCYNL-EPKNETETDVGWR 373
            P +P+  +C  +   + S+S  L  I   +   + Y G++ C N+ E    +   +GW 
Sbjct: 307 LPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWS 366

Query: 374 WQKCSEMVMPIGTDN-DTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHL 433
           +Q C+E+VMP  T+  D MF P++++L+   + C Q +GV PRP W+TT YGG +I  H 
Sbjct: 367 YQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSH- 426

Query: 434 QRFGSNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRE 487
               +NI+FSNG  DP+S GGV + ++D+L+AV  + G+H LD+   N  DP  ++  R 
Sbjct: 427 ----TNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARS 486

BLAST of CmaCh20G005370 vs. Swiss-Prot
Match: PCP_BOVIN (Lysosomal Pro-X carboxypeptidase OS=Bos taurus GN=PRCP PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.1e-81
Identity = 179/486 (36.83%), Postives = 274/486 (56.38%), Query Frame = 1

Query: 28  RIPRLSPFSRTFLPNTKASPVSDDFKTFYYNQTLDHFNYKPESYTSFPHRYIINFNYWGG 87
           R P   P+S +F        ++  +   Y  Q +DHF +  +   +F  RY+I  NYW  
Sbjct: 29  RAPSSLPWSTSF---RSRPTITLKYSIRYIQQKVDHFGFNIDR--TFKQRYLIADNYWKE 88

Query: 88  ANSSAPILAYLGAEGPLEGDLNAIGFMTDNALQFDALLVYIEHRYYGKSIPFGSRQEALK 147
              S  IL Y G EG +    N  GFM D A +  A+LV+ EHRYYG+S+PFG+  ++  
Sbjct: 89  DGGS--ILFYTGNEGDIIWFCNNTGFMWDIAEEMKAMLVFAEHRYYGESLPFGA--DSFS 148

Query: 148 NASTLGYFNSAQAIADYAAVLIHLKKKLH-AKDCPVIVLGGSYGGMLAAWFRLKYPNVAL 207
           ++  L +  + QA+AD+A ++ +LK+ +  A++  VI LGGSYGGMLAAWFR+KYP++ +
Sbjct: 149 DSRHLNFLTTEQALADFAKLIRYLKRTIPGARNQHVIALGGSYGGMLAAWFRMKYPHLVV 208

Query: 208 GALASSAPILYFDDITPHNGYYSIATKDFKEVSESCYETIRDSWSEMEAMASKPNGLSIL 267
           GALASSAPI  F+D+ P + +  I T DF +   +C E+IR SW  +  +A K  GL  L
Sbjct: 209 GALASSAPIWQFNDLVPCDIFMKIVTTDFSQSGPNCSESIRRSWDAINRLAKKGTGLRWL 268

Query: 268 SKEFKTCSPL---NSYSQLEDYLWSMYAGAAQYNQP---------PRYPVTRICGGIDGA 327
           S+    C+PL       +L+D++   +   A  + P         P +PV  +C     +
Sbjct: 269 SEALHLCTPLTKSQDVQRLKDWISETWVNVAMVDYPYESNFLQPLPAWPVKVVCQYFKYS 328

Query: 328 SSESGTLGK---IAAGV-FAYEGKLSCYNLEPKNETETD----VGWRWQKCSEMVMPIGT 387
           +     + +    A  V + Y G+  C N+   +ET T     +GW +Q C+EMVMP  +
Sbjct: 329 NVPDTVMVQNIFQALNVYYNYSGQAKCLNV---SETATSSLGVLGWSYQACTEMVMPTCS 388

Query: 388 DN-DTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGSNIIFSNGL 447
           D  D MF P++++++ + + C + +GV PRP W+ T YGG +I  H     +NIIFSNG 
Sbjct: 389 DGVDDMFEPHSWNMKEYSDDCFKQWGVRPRPSWIPTMYGGKNISSH-----TNIIFSNGE 448

Query: 448 RDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVSIIKEWIRKY 492
            DP+S GGV + ++D+LLA+   NG+H LD+  +N  DP  +   R  EV  +K+WI  +
Sbjct: 449 LDPWSGGGVTKDITDTLLAIVIPNGAHHLDLRASNALDPVSVQLTRSLEVKYMKQWISDF 497

BLAST of CmaCh20G005370 vs. Swiss-Prot
Match: DPP2_RAT (Dipeptidyl peptidase 2 OS=Rattus norvegicus GN=Dpp7 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.1e-65
Identity = 155/470 (32.98%), Postives = 247/470 (52.55%), Query Frame = 1

Query: 40  LPNTKASPVSDDFKTFYYNQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLG 99
           L  T  S +  DF+  Y+ Q +DHFN++  S  +F  R++++  +W       PI  Y G
Sbjct: 29  LQATADSVLDPDFRENYFEQYMDHFNFESFSNKTFGQRFLVSDKFW--KMGEGPIFFYTG 88

Query: 100 AEGPLEGDLNAIGFMTDNALQFDALLVYIEHRYYGKSIPFG--SRQEALKNASTLGYFNS 159
            EG +    N  GF+ + A Q +ALLV+ EHRYYGKS+PFG  S Q       T+     
Sbjct: 89  NEGDIWSLANNSGFIVELAAQQEALLVFAEHRYYGKSLPFGVQSTQRGYTQLLTV----- 148

Query: 160 AQAIADYAAVLIHLKKKLHAKDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILY 219
            QA+AD+A +L  L+  L  +D P I  GGSYGGML+A+ R+KYP++  GALA+SAP++ 
Sbjct: 149 EQALADFAVLLQALRHNLGVQDAPTIAFGGSYGGMLSAYMRMKYPHLVAGALAASAPVIA 208

Query: 220 FDDITPHNGYYSIATKDFKEVSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLN 279
              +   + ++   T DF   S  C + +RD++ +++ +  +      +S+ F TC  L+
Sbjct: 209 VAGLGNPDQFFRDVTADFYGQSPKCAQAVRDAFQQIKDLFLQ-GAYDTISQNFGTCQSLS 268

Query: 280 S---YSQLEDYLWSMYAGAAQYNQP---------PRYPVTRICGGIDGASSESGTLGKIA 339
           S    +QL  +  + +   A  + P         P  PV   C  +         L  +A
Sbjct: 269 SPKDLTQLFGFARNAFTVLAMMDYPYPTNFLGPLPANPVKVGCERLLSEGQRIMGLRALA 328

Query: 340 AGVFAYEGKLSCYNLEPKNETETDV----------GWRWQKCSEMVMPIGTDNDT-MFP- 399
             V+   G   C+++    ++  D            W +Q C+E+ +   ++N T MFP 
Sbjct: 329 GLVYNSSGMEPCFDIYQMYQSCADPTGCGTGSNARAWDYQACTEINLTFDSNNVTDMFPE 388

Query: 400 -PYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGSNIIFSNGLRDPYSSG 459
            P++ +LR    +C   +GV PRP W+ T + G D+K       SNIIFSNG  DP++ G
Sbjct: 389 IPFSDELRQ--QYCLDTWGVWPRPDWLQTSFWGGDLKA-----ASNIIFSNGDLDPWAGG 448

Query: 460 GVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVSIIKEWI 483
           G+ ++LS S++AV    G+H LD+  +N  DP  +V+ R+ E ++I+EW+
Sbjct: 449 GIQRNLSTSIIAVTIQGGAHHLDLRASNSEDPPSVVEVRKLEATLIREWV 483

BLAST of CmaCh20G005370 vs. TrEMBL
Match: A0A0A0KAY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G149410 PE=4 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 4.0e-256
Identity = 427/497 (85.92%), Postives = 460/497 (92.56%), Query Frame = 1

Query: 1   MNFPMFSS-PWLPFILLILSTCVTATQYRIPRLSPFSRTFLPNTKASP--VSDDFKTFYY 60
           M+FPMFSS PWLPFIL ILS CVTATQYRIPRLSP  RTFL N +A P  +SDDFKTFYY
Sbjct: 1   MSFPMFSSSPWLPFILFILSNCVTATQYRIPRLSPIGRTFLHNAEAIPSSISDDFKTFYY 60

Query: 61  NQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN 120
           NQTLDHFNY+PESYT FPHRYIINF YWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN
Sbjct: 61  NQTLDHFNYRPESYTCFPHRYIINFKYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN 120

Query: 121 ALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHA 180
           A +FDALLVYIEHRYYGKS+PFGSR+EALKNASTLGYF+SAQAIADYAAVLIHLK+K HA
Sbjct: 121 AARFDALLVYIEHRYYGKSMPFGSREEALKNASTLGYFSSAQAIADYAAVLIHLKQKYHA 180

Query: 181 KDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKE 240
           KD PVIVLGGSYGGMLAAWFRLKYP+VALGALASSAPILYF+DITPHNGYYSIATKDF+E
Sbjct: 181 KDSPVIVLGGSYGGMLAAWFRLKYPHVALGALASSAPILYFEDITPHNGYYSIATKDFRE 240

Query: 241 VSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYN 300
           VSE+CYETIRDSWS++E + SKPNGLSILSKEFKTCSPLNS SQLEDYLWSMYAGAAQYN
Sbjct: 241 VSETCYETIRDSWSKIEIIGSKPNGLSILSKEFKTCSPLNSSSQLEDYLWSMYAGAAQYN 300

Query: 301 QPPRYPVTRICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQKC 360
            PPRYPVTRICGGIDGAS  SG + K+AAGVFAY+G LSCYN+ P++ETETDVGWRWQ+C
Sbjct: 301 HPPRYPVTRICGGIDGASPGSGIISKVAAGVFAYKGNLSCYNIGPRSETETDVGWRWQRC 360

Query: 361 SEMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGS 420
           SEMVMP+ T NDTMFPP TFDL+SF+++C QLYGVS RPHWVTTYYGGNDIKL LQRFGS
Sbjct: 361 SEMVMPLSTTNDTMFPPITFDLKSFVDYCYQLYGVSSRPHWVTTYYGGNDIKLILQRFGS 420

Query: 421 NIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVSI 480
           NIIFSNGLRDPYSSGGVLQ+LSDSLLAVHT  GSHCLDILRANETDPQWLVKQRETEV I
Sbjct: 421 NIIFSNGLRDPYSSGGVLQNLSDSLLAVHTPKGSHCLDILRANETDPQWLVKQRETEVRI 480

Query: 481 IKEWIRKYYADLKEFKQ 495
           I+ WI KYYADL++ K+
Sbjct: 481 IEGWISKYYADLEKSKK 497

BLAST of CmaCh20G005370 vs. TrEMBL
Match: A0A0A0KBK9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G149390 PE=4 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 5.4e-221
Identity = 368/498 (73.90%), Postives = 426/498 (85.54%), Query Frame = 1

Query: 1   MNFPMFSSPWLPFILLILSTCV-TATQY-RIPRLSPFSRTFLPNTKA--SPVSDDFKTFY 60
           M FPMFSSPW+P +L + ST V T+ Q+ R PRLSP    FL +++   S   DDFKT+Y
Sbjct: 15  MRFPMFSSPWIPLLLFVFSTSVVTSLQHNRFPRLSPVGEKFLHHSRVLNSLPLDDFKTYY 74

Query: 61  YNQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTD 120
           YNQTLDHFNY+PESYT+FP RYIINF YWGG NSSAPI AYLGAE P++ DL+ IGFMTD
Sbjct: 75  YNQTLDHFNYRPESYTTFPQRYIINFKYWGGPNSSAPIFAYLGAEAPIDDDLDFIGFMTD 134

Query: 121 NALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLH 180
           NA+QF+ALL+YIEHRYYGKSIPF SR EAL NASTLGYFNSAQAIADYAA+LIH+KK+ H
Sbjct: 135 NAIQFNALLIYIEHRYYGKSIPFRSRDEALGNASTLGYFNSAQAIADYAAILIHVKKEFH 194

Query: 181 AKDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFK 240
           A   PVIV+GGSYGGMLA+WFRLKYP+VALGALASSAPILYFDDITP +GYYS+ TKDF+
Sbjct: 195 ANYSPVIVIGGSYGGMLASWFRLKYPHVALGALASSAPILYFDDITPQDGYYSVVTKDFR 254

Query: 241 EVSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQY 300
            +SE+CYETI+ SWSE+E +A +PNGLSIL +EFKTC PL  Y +LEDYLWSMYA AAQY
Sbjct: 255 GLSETCYETIKKSWSEIETVAYQPNGLSILDQEFKTCRPLRGYFELEDYLWSMYASAAQY 314

Query: 301 NQPPRYPVTRICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQK 360
           N PP+YPVTRIC  IDG  S +GTL KIAAGVFA+ G +SCY  EP+NETETDVGWRWQ 
Sbjct: 315 NHPPKYPVTRICDAIDGTYSVNGTLSKIAAGVFAFRGSVSCYINEPRNETETDVGWRWQS 374

Query: 361 CSEMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFG 420
           CSEMVMPIG+D+D MFPP  FDL+S IN+CN+LYGV PRPHW TTYYGG+DI+L LQRFG
Sbjct: 375 CSEMVMPIGSDDD-MFPPSPFDLQSVINYCNRLYGVPPRPHWATTYYGGHDIRLVLQRFG 434

Query: 421 SNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVS 480
           SNIIFSNGL+DPYS  GVL ++SDSLLAV+T NGSHCLDIL+A+ETDP+WLV+QR+TEV 
Sbjct: 435 SNIIFSNGLKDPYSIAGVLHNISDSLLAVYTTNGSHCLDILKAHETDPEWLVRQRKTEVG 494

Query: 481 IIKEWIRKYYADLKEFKQ 495
           IIK WI +YYADLK++KQ
Sbjct: 495 IIKGWISEYYADLKKYKQ 511

BLAST of CmaCh20G005370 vs. TrEMBL
Match: A0A0A0KDH5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G149400 PE=4 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 3.0e-219
Identity = 370/492 (75.20%), Postives = 419/492 (85.16%), Query Frame = 1

Query: 5   MFSSPWLPFILLILSTCVTATQYRIPRLSPFSRTFLPNTKAS--PVSDDFKTFYYNQTLD 64
           MFSSPWLPF+LL LS  VTA Q+RIPRLSP    FL ++KA   P SDDFKTFY+NQTLD
Sbjct: 1   MFSSPWLPFLLLFLSNSVTAFQFRIPRLSPIGEKFLHHSKALELPPSDDFKTFYFNQTLD 60

Query: 65  HFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDNALQFD 124
           HFNY+PESYT+FP RYIINF YWGGANSSAPILAYLG E P++  +N IGFMTDNA++F+
Sbjct: 61  HFNYRPESYTTFPQRYIINFKYWGGANSSAPILAYLGPEAPIDSAMNVIGFMTDNAVKFN 120

Query: 125 ALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHAKDCPV 184
           ALLVYIEHRYYGKSIPFGSR+EAL+NASTLGYFNSAQA+ADYAA+LIH+KK+  AK  PV
Sbjct: 121 ALLVYIEHRYYGKSIPFGSRKEALRNASTLGYFNSAQALADYAAILIHVKKEFSAKYSPV 180

Query: 185 IVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKEVSESC 244
           IV+GGSYGGMLA WFRLKYP+VALGALASSAPILYF+DITP NGYY I TKDF+EVS++C
Sbjct: 181 IVIGGSYGGMLATWFRLKYPHVALGALASSAPILYFNDITPENGYYVIVTKDFREVSQTC 240

Query: 245 YETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYNQPPRY 304
           YE+IR+SWSE+E +AS+ NGLS+L K FKTCSPL S +QLE+YLW MYA AAQYN P RY
Sbjct: 241 YESIRESWSEIETVASQSNGLSVLDKVFKTCSPLRSSTQLENYLWFMYASAAQYNHPSRY 300

Query: 305 PVTRICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQKCSEMVM 364
           PV RIC  ID   S +GTLGKIAAGVFAY G+LSCY  EP N TET VGW+WQ+CSEMVM
Sbjct: 301 PVNRICDAIDQTYS-NGTLGKIAAGVFAYRGELSCYINEPINTTETTVGWQWQRCSEMVM 360

Query: 365 PIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGSNIIFS 424
           PI T NDTMFP  TFD  SF  +CNQLYGV+PRPHWVTTYYGG+DI L L RF SNIIFS
Sbjct: 361 PISTGNDTMFPSETFDHESFSIYCNQLYGVTPRPHWVTTYYGGHDIHLILHRFASNIIFS 420

Query: 425 NGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVSIIKEWI 484
           NGL+DPYS GGVL ++SDSLLAV+TANGSHCLDIL AN  DP+WLV QR+TEV IIKEWI
Sbjct: 421 NGLKDPYSIGGVLHNISDSLLAVYTANGSHCLDILTANRMDPEWLVTQRKTEVGIIKEWI 480

Query: 485 RKYYADLKEFKQ 495
            +YYADL  +K+
Sbjct: 481 DEYYADLANYKK 491

BLAST of CmaCh20G005370 vs. TrEMBL
Match: B9HQR7_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s00740g PE=4 SV=2)

HSP 1 Score: 695.3 bits (1793), Expect = 5.5e-197
Identity = 326/487 (66.94%), Postives = 399/487 (81.93%), Query Frame = 1

Query: 11  LPFILLILSTCVTATQ-YRIPRLSPFS-RTFLPNTK---ASPVSDDFKTFYYNQTLDHFN 70
           LP +LL   T  TA + + IPRLSP   R +L +        V +DF+TF+YNQTLDHFN
Sbjct: 9   LPLLLLFSLTTATAKRLHTIPRLSPIGPRVWLDHPDQILGESVREDFETFFYNQTLDHFN 68

Query: 71  YKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDNALQFDALL 130
           Y+PESY +F  RY+IN  YWGGAN+SAPIL YLGAE P++GDL+A+GF+ D A++F++LL
Sbjct: 69  YRPESYDTFLQRYLINSKYWGGANASAPILVYLGAEAPIDGDLDAVGFLVDTAVEFNSLL 128

Query: 131 VYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHAKDCPVIVL 190
           VY+EHRYYGKSIPFGSR+EALKNASTLGYFNSAQAIADYAA++IH+KK L AKD PVIV+
Sbjct: 129 VYVEHRYYGKSIPFGSREEALKNASTLGYFNSAQAIADYAAIIIHIKKTLQAKDSPVIVI 188

Query: 191 GGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKEVSESCYET 250
           GGSYGGMLA+WFRLKYP++ALGALASSAP+LYFDDITP  GYY++ +KDF+  SE+CY+T
Sbjct: 189 GGSYGGMLASWFRLKYPHIALGALASSAPVLYFDDITPQYGYYALVSKDFRGASETCYQT 248

Query: 251 IRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYNQPPRYPVT 310
           IR+SW E++ +ASKP+GLSILSK+FKTC+PL   S+L+++L SMYA AAQYN+PP YPV 
Sbjct: 249 IRESWEEIDEVASKPDGLSILSKKFKTCNPLTDASELKNHLDSMYANAAQYNKPPTYPVN 308

Query: 311 RICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQKCSEMVMPIG 370
           ++CGGIDG       LG++  G+ AY+G  SCY  EP N++ET VGWRWQ CSEMVMPIG
Sbjct: 309 KVCGGIDGCGFGDDLLGRVFGGLVAYKGNRSCYVNEPTNQSETSVGWRWQTCSEMVMPIG 368

Query: 371 TDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGSNIIFSNGL 430
             ND+MFPP  FDL+++I  C  LY V+PR HWVTTYYGG+ I+L LQRF SNIIFSNGL
Sbjct: 369 YGNDSMFPPDPFDLKAYIEDCKSLYDVTPRFHWVTTYYGGHSIRLILQRFASNIIFSNGL 428

Query: 431 RDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVSIIKEWIRKY 490
           RDPYSSGGVL+++SD+++AV T NGSHCLDIL A ETDP+WLV QR+TE+ IIKEWI KY
Sbjct: 429 RDPYSSGGVLENISDTVVAVKTVNGSHCLDILFAKETDPEWLVAQRKTEIKIIKEWINKY 488

Query: 491 YADLKEF 493
           YADL  F
Sbjct: 489 YADLSRF 495

BLAST of CmaCh20G005370 vs. TrEMBL
Match: A0A061GWT8_THECC (Serine carboxypeptidase S28 family protein OS=Theobroma cacao GN=TCM_046935 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 1.3e-195
Identity = 334/500 (66.80%), Postives = 397/500 (79.40%), Query Frame = 1

Query: 1   MNFPMFSSPWLPFILLILSTCVTATQYRIPRLSPFSRTFL--PNTKASPVSDDFKTFYYN 60
           MN P+ SS WL  I++I+S  VTA  ++IPRLSP   T L  P   ++PVS+D +TFYY 
Sbjct: 1   MNSPVISSQWLRLIIMIVSMAVTAAHFKIPRLSPTLGTILEQPEILSAPVSEDLRTFYYT 60

Query: 61  QTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDNA 120
           QTLDHFNY PESYT+F  RY++N  YWGGAN SAPILAYLGAE PL+G   AIGF+ DNA
Sbjct: 61  QTLDHFNYNPESYTTFQQRYVMNSKYWGGANVSAPILAYLGAESPLDGTPAAIGFLNDNA 120

Query: 121 LQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHAK 180
           ++F AL+VYIEHRYYGKSIPFGSR+EA +NASTLGYFNSAQAIADYAA+++H+KKKL A+
Sbjct: 121 IRFKALIVYIEHRYYGKSIPFGSREEAFQNASTLGYFNSAQAIADYAAIIMHIKKKLQAR 180

Query: 181 DCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDI--TPHNGYYSIATKDFK 240
             PVIV+GGSYGGMLA+WFRLKYP+VALGALASSAPILYFD+I   P  GYYS+ TKDF+
Sbjct: 181 YSPVIVIGGSYGGMLASWFRLKYPHVALGALASSAPILYFDEIPLQPEGGYYSVVTKDFR 240

Query: 241 EVSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQY 300
           E SE+CY+TI+ SWSE+  +ASKP+GLS LSK+FKTC PL S S+L+ +L  MYA  AQY
Sbjct: 241 EASETCYQTIQKSWSEINRVASKPHGLSTLSKKFKTCYPLTSSSELKSFLRLMYAYTAQY 300

Query: 301 NQPPRYPVTRICGGIDGAS--SESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRW 360
           N+PPRYPV+ +CGGIDGAS  S+   L KI +GV AY G  SCY     N +E ++GW W
Sbjct: 301 NRPPRYPVSVVCGGIDGASFGSQDDILTKIFSGVVAYYGNRSCYVNPETNASEIEIGWSW 360

Query: 361 QKCSEMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQR 420
           Q+CSEMV+PIG  N TM     F+L SFI  C   YGV  RPHWVT+YYGG+DIKL L R
Sbjct: 361 QRCSEMVIPIGIGNGTMLEASPFNLTSFIKQCESFYGVPSRPHWVTSYYGGHDIKLILHR 420

Query: 421 FGSNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETE 480
           FGSNIIFSNGLRDPYSSGGVL+++S+S+LAV T NGSHCLDIL   ETDP+WL++QR+ E
Sbjct: 421 FGSNIIFSNGLRDPYSSGGVLENISNSILAVSTVNGSHCLDILAERETDPEWLIRQRKIE 480

Query: 481 VSIIKEWIRKYYADLKEFKQ 495
           V IIK WI KYYADLK FKQ
Sbjct: 481 VKIIKGWIAKYYADLKAFKQ 500

BLAST of CmaCh20G005370 vs. TAIR10
Match: AT5G22860.1 (AT5G22860.1 Serine carboxypeptidase S28 family protein)

HSP 1 Score: 553.5 bits (1425), Expect = 1.3e-157
Identity = 270/495 (54.55%), Postives = 355/495 (71.72%), Query Frame = 1

Query: 11  LPFILLILSTCVTATQYRIP-------RLSPFSRTFL--PNTKASPVSD-DFKTFYYNQT 70
           LP+ +LIL    T++ Y IP       RL   S+T    P+     V + + K +Y+NQT
Sbjct: 3   LPYTILILFIFSTSSSYLIPLAHSKIARLGISSKTLKNEPDGSTQKVDESNLKMYYFNQT 62

Query: 71  LDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDNALQ 130
           LDHF + PESY +F  RY I+  +WGGA ++APILA+LG E  L+ DL AIGF+ DN  +
Sbjct: 63  LDHFTFTPESYMTFQQRYAIDSTHWGGAKANAPILAFLGEESSLDSDLAAIGFLRDNGPR 122

Query: 131 FDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHAKDC 190
            +ALLVYIEHRYYG+++PFGS +EALKNASTLGY N+AQA+ADYAA+L+H+K+K      
Sbjct: 123 LNALLVYIEHRYYGETMPFGSAEEALKNASTLGYLNAAQALADYAAILLHVKEKYSTNHS 182

Query: 191 PVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKEVSE 250
           P+IV+GGSYGGMLAAWFRLKYP++ALGALASSAP+LYF+D  P  GYY I TK FKE SE
Sbjct: 183 PIIVIGGSYGGMLAAWFRLKYPHIALGALASSAPLLYFEDTRPKFGYYYIVTKVFKEASE 242

Query: 251 SCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYNQPP 310
            CY TIR+SW E++ +A KPNGLSILSK+FKTC+PLN    ++D+L ++YA A QYN+ P
Sbjct: 243 RCYNTIRNSWIEIDRVAGKPNGLSILSKQFKTCAPLNGSFDIKDFLDTIYAEAVQYNRGP 302

Query: 311 RYPVTRICGGIDG--ASSESGTLGKIAAGVFAYEGKLSCYNLEP-KNETETDVGWRWQKC 370
            + V ++C  I+    +     L +I AGV A  G  +CY+ +     T  ++ WRWQ C
Sbjct: 303 NFWVAKVCNAINANPPNRRYNLLDRIFAGVVALVGNRTCYDTKMFAQPTNNNIAWRWQSC 362

Query: 371 SEMVMPIGTD-NDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFG 430
           SE+VMP+G D  DTMFP   F++ S+I+ C   +GV+PRPHW+TTY+G  ++KL LQ+FG
Sbjct: 363 SEIVMPVGYDKQDTMFPTAPFNMTSYIDGCKSYHGVTPRPHWITTYFGIQEVKLILQKFG 422

Query: 431 SNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVS 490
           SNIIFSNGL DPYS GGVL+ +SD+L+A+ T NGSHCLDI   ++ DP+WLV QRE E+ 
Sbjct: 423 SNIIFSNGLSDPYSVGGVLEDISDTLVAITTKNGSHCLDITLKSKEDPEWLVIQREKEIK 482

Query: 491 IIKEWIRKYYADLKE 492
           +I  WI  Y  DL++
Sbjct: 483 VIDSWISTYQNDLRD 497

BLAST of CmaCh20G005370 vs. TAIR10
Match: AT2G24280.1 (AT2G24280.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 429.5 bits (1103), Expect = 2.8e-120
Identity = 210/452 (46.46%), Postives = 293/452 (64.82%), Query Frame = 1

Query: 52  FKTFYYNQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAI 111
           F+T Y+ Q LDHF++ P+SY  F  +Y+IN  +W       PI  Y G EG ++   +  
Sbjct: 46  FETRYFPQNLDHFSFTPDSYKVFHQKYLINNRFW---RKGGPIFVYTGNEGDIDWFASNT 105

Query: 112 GFMTDNALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHL 171
           GFM D A +F ALLV+IEHR+YG+S PFG +    K+A TLGY NS QA+ADYA ++  L
Sbjct: 106 GFMLDIAPKFRALLVFIEHRFYGESTPFGKKSH--KSAETLGYLNSQQALADYAILIRSL 165

Query: 172 KKKLHAKDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIA 231
           K+ L ++  PV+V GGSYGGMLAAWFRLKYP++ +GALASSAPIL+FD+I P   +Y   
Sbjct: 166 KQNLSSEASPVVVFGGSYGGMLAAWFRLKYPHITIGALASSAPILHFDNIVPLTSFYDAI 225

Query: 232 TKDFKEVSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYA 291
           ++DFK+ S +C++ I+ SW E+EA+++  NGL  LSK+F+TC  L+S     D+L   + 
Sbjct: 226 SQDFKDASINCFKVIKRSWEELEAVSTMKNGLQELSKKFRTCKGLHSQYSARDWLSGAFV 285

Query: 292 GAAQYNQP---------PRYPVTRICGGIDGASSESGTLGKIAAGV---FAYEGKLSCYN 351
             A  N P         P YPV ++C  IDG    S  L +  A     + Y G   C+ 
Sbjct: 286 YTAMVNYPTAANFMAPLPGYPVEQMCKIIDGFPRGSSNLDRAFAAASLYYNYSGSEKCFE 345

Query: 352 LEPKNETETDVGWRWQKCSEMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWV 411
           +E + +     GW++Q C+EMVMP+   N +M PPY  D  +F   C   YGV PRPHW+
Sbjct: 346 MEQQTDDHGLDGWQYQACTEMVMPMSCSNQSMLPPYENDSEAFQEQCMTRYGVKPRPHWI 405

Query: 412 TTYYGGNDIKLHLQRFGSNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRA 471
           TT +GG  I+  L+RFGSNIIFSNG++DP+S GGVL+++S S++A+ T  G+H  D+  A
Sbjct: 406 TTEFGGMRIETVLKRFGSNIIFSNGMQDPWSRGGVLKNISSSIVALVTKKGAHHADLRAA 465

Query: 472 NETDPQWLVKQRETEVSIIKEWIRKYYADLKE 492
            + DP+WL +QR  EV+II++WI +YY DL+E
Sbjct: 466 TKDDPEWLKEQRRQEVAIIEKWISEYYRDLRE 492

BLAST of CmaCh20G005370 vs. TAIR10
Match: AT5G65760.1 (AT5G65760.1 Serine carboxypeptidase S28 family protein)

HSP 1 Score: 418.3 bits (1074), Expect = 6.5e-117
Identity = 212/455 (46.59%), Postives = 295/455 (64.84%), Query Frame = 1

Query: 52  FKTFYYNQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAI 111
           ++T +++Q LDHF++       F  RY+IN ++W GA++  PI  Y G EG +E      
Sbjct: 58  YETKFFSQQLDHFSFA--DLPKFSQRYLINSDHWLGASALGPIFLYCGNEGDIEWFATNS 117

Query: 112 GFMTDNALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHL 171
           GF+ D A +F ALLV+ EHRYYG+S+P+GSR+EA KNA+TL Y  + QA+AD+A  +  L
Sbjct: 118 GFIWDIAPKFGALLVFPEHRYYGESMPYGSREEAYKNATTLSYLTTEQALADFAVFVTDL 177

Query: 172 KKKLHAKDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIA 231
           K+ L A+ CPV++ GGSYGGMLAAW RLKYP++A+GALASSAPIL F+D+ P   +Y IA
Sbjct: 178 KRNLSAEACPVVLFGGSYGGMLAAWMRLKYPHIAIGALASSAPILQFEDVVPPETFYDIA 237

Query: 232 TKDFKEVSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYA 291
           + DFK  S SC+ TI+DSW  + A   K NGL  L+K F  C  LNS   L D+L S Y+
Sbjct: 238 SNDFKRESSSCFNTIKDSWDAIIAEGQKENGLLQLTKTFHFCRVLNSTDDLSDWLDSAYS 297

Query: 292 GAAQYNQP---------PRYPVTRICGGIDGASSESGTLGKIAAGV---FAYEGKLSCYN 351
             A  + P         P +P+  +C  IDGA S +  L +I AG+   + Y G + C+ 
Sbjct: 298 YLAMVDYPYPADFMMPLPGHPIREVCRKIDGAGSNASILDRIYAGISVYYNYTGNVDCFK 357

Query: 352 LEPKNETETDVGWRWQKCSEMVMPIGTDND-TMFPPYTFDLRSFINHCNQLYGVSPRPHW 411
           L+  ++     GW WQ C+EMVMP+ ++ + +MFP Y F+  S+   C   + V+PRP W
Sbjct: 358 LD--DDPHGLDGWNWQACTEMVMPMSSNQENSMFPGYGFNYSSYKEECWNTFRVNPRPKW 417

Query: 412 VTTYYGGNDIKLHLQRFGSNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILR 471
           VTT +GG+DI   L+ FGSNIIFSNGL DP+S G VL++LSD+++A+ T  G+H LD+  
Sbjct: 418 VTTEFGGHDIATTLKSFGSNIIFSNGLLDPWSGGSVLKNLSDTIVALVTKEGAHHLDLRP 477

Query: 472 ANETDPQWLVKQRETEVSIIKEWIRKYYADLKEFK 494
           +   DP+WLV QRE E+ +I+ WI  Y  + KE K
Sbjct: 478 STPEDPKWLVDQREAEIRLIQGWIETYRVE-KEAK 507

BLAST of CmaCh20G005370 vs. TAIR10
Match: AT3G28680.1 (AT3G28680.1 Serine carboxypeptidase S28 family protein)

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-39
Identity = 82/173 (47.40%), Postives = 115/173 (66.47%), Query Frame = 1

Query: 187 GSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKEVSESCYETI 246
           G+   +LAAWF+LKYP +ALGALASSAP+LYF+D  P +GY+ I TK FKE+S+ C+  I
Sbjct: 18  GAVHKVLAAWFKLKYPYIALGALASSAPLLYFEDTLPKHGYFYIVTKVFKEMSKECHNKI 77

Query: 247 RDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYNQPPRYPVTR 306
             SW E++ +A+KPN LSILSK FK C+PLN   +L+ Y+  +YA  AQY+   ++ V R
Sbjct: 78  HKSWDEIDRIAAKPNSLSILSKNFKLCNPLNDIIELKSYVSYIYARTAQYSD-NQFSVAR 137

Query: 307 ICGGIDGA--SSESGTLGKIAAGVFAYEGKLSCYNLEPKN--ETETDVGWRWQ 356
           +C  I+ +  +++S  L +I AGV A  G +SCY +   +   T  D  W WQ
Sbjct: 138 LCEAINTSPPNTKSDLLDQIFAGVVASRGNISCYGMSSPSYQMTNDDRAWGWQ 189

BLAST of CmaCh20G005370 vs. TAIR10
Match: AT4G36195.1 (AT4G36195.1 Serine carboxypeptidase S28 family protein)

HSP 1 Score: 114.0 bits (284), Expect = 2.6e-25
Identity = 109/395 (27.59%), Postives = 170/395 (43.04%), Query Frame = 1

Query: 56  YYNQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMT 115
           ++NQTLDH  Y P  +  F  RY    ++    +   PI   +  EGP  G  N   ++T
Sbjct: 49  WFNQTLDH--YSPSDHREFKQRYYEYLDHLRVPDG--PIFMMICGEGPCNGIPN--DYIT 108

Query: 116 DNALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKL 175
             A +FDA +V +EHRYYGKS PF S          L Y +S QA+ D AA   + +  L
Sbjct: 109 VLAKKFDAGIVSLEHRYYGKSSPFKSLA-----TENLKYLSSKQALFDLAAFRQYYQDSL 168

Query: 176 HAK-------DCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYY 235
           + K       + P    G SY G L+AWFRLK+P++  G+LASSA +            Y
Sbjct: 169 NVKFNRSGDVENPWFFFGASYSGALSAWFRLKFPHLTCGSLASSAVV---------RAVY 228

Query: 236 SIATKDFKEVSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWS 295
                D +++ ES     + +  E   +     GL + ++  K            D+L+ 
Sbjct: 229 EFPEFD-QQIGESAGPECKAALQETNKLLEL--GLKVNNRAVKALFNATELDVDADFLYL 288

Query: 296 MYAG---AAQYNQPPRYPVTRI---CGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEP 355
           +      A QY  P +  V  +       D   + +  + +   GVF    K   Y+ + 
Sbjct: 289 IADAEVMAIQYGNPDKLCVPLVEAQKNRDDLVEAYAKYVREFCVGVFGLSSK--TYSRKH 348

Query: 356 KNET-----ETDVGWRWQKCSEMV-MPIGTDNDTMFPPYTFDLRSFINHCNQLY--GVSP 415
             +T       D  W +Q C+E+    +   ND++   +  +    ++ C  L+  GV P
Sbjct: 349 LLDTAVTPESADRLWWFQVCTEVAYFQVAPANDSI-RSHQINTEYHLDLCKSLFGKGVYP 408

Query: 416 RPHWVTTYYGGNDIKLHLQRFGSNIIFSNGLRDPY 430
                  YYG + I        + IIF+NG +DP+
Sbjct: 409 EVDATNLYYGSDRIA------ATKIIFTNGSQDPW 411

BLAST of CmaCh20G005370 vs. NCBI nr
Match: gi|659117555|ref|XP_008458663.1| (PREDICTED: lysosomal Pro-X carboxypeptidase isoform X1 [Cucumis melo])

HSP 1 Score: 892.5 bits (2305), Expect = 3.3e-256
Identity = 426/497 (85.71%), Postives = 463/497 (93.16%), Query Frame = 1

Query: 1   MNFPMFSS-PWLPFILLILSTCVTATQYRIPRLSPFSRTFLPNTKA--SPVSDDFKTFYY 60
           M+FPMFSS PW+PFIL ILS CVTATQYRIPRLSP  RTFL N +A  S +SDDFKTFYY
Sbjct: 1   MSFPMFSSSPWVPFILFILSNCVTATQYRIPRLSPIGRTFLHNAEAISSSISDDFKTFYY 60

Query: 61  NQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN 120
           NQ+LDHFNY+PESYT FPHRYIINF YWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN
Sbjct: 61  NQSLDHFNYRPESYTCFPHRYIINFKYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN 120

Query: 121 ALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHA 180
           A++FDALLVYIEHRYYGKS+PFGSR+EALKNASTLGYF+SAQAIADYAAVL+HLK+K HA
Sbjct: 121 AVRFDALLVYIEHRYYGKSMPFGSREEALKNASTLGYFSSAQAIADYAAVLLHLKQKYHA 180

Query: 181 KDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKE 240
           KD PVIVLGGSYGGMLAAWFRLKYP+VALGALASSAPILYF+DITPHNGYYSIATKDF+E
Sbjct: 181 KDSPVIVLGGSYGGMLAAWFRLKYPHVALGALASSAPILYFEDITPHNGYYSIATKDFRE 240

Query: 241 VSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYN 300
           VSE+CYETIRDSWS++E +ASKPNGLSILSKEFKTCSPLNS SQLEDYLWSMYAGAAQYN
Sbjct: 241 VSETCYETIRDSWSKIETIASKPNGLSILSKEFKTCSPLNSSSQLEDYLWSMYAGAAQYN 300

Query: 301 QPPRYPVTRICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQKC 360
            PPRYPVTRICGGIDGAS  SG + K+AAGVFAY+G L CYN+ P+N+TETDVGWRWQ+C
Sbjct: 301 HPPRYPVTRICGGIDGASPGSGIISKVAAGVFAYKGNLPCYNIGPRNDTETDVGWRWQRC 360

Query: 361 SEMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGS 420
           SEMVMP+ T NDTMFPP TFDLRSFI++C QLYGVSPRPHWVTTYYGGNDIKL LQRFGS
Sbjct: 361 SEMVMPMSTSNDTMFPPITFDLRSFIDYCYQLYGVSPRPHWVTTYYGGNDIKLILQRFGS 420

Query: 421 NIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVSI 480
           NIIFSNGLRDPYSSGGVLQ+LSDSLLAVHT NGSHCLDILRANETDPQWLV+QRE EVSI
Sbjct: 421 NIIFSNGLRDPYSSGGVLQNLSDSLLAVHTLNGSHCLDILRANETDPQWLVEQREKEVSI 480

Query: 481 IKEWIRKYYADLKEFKQ 495
           I+ WI +YYADL++ K+
Sbjct: 481 IEGWISQYYADLEKSKK 497

BLAST of CmaCh20G005370 vs. NCBI nr
Match: gi|449456064|ref|XP_004145770.1| (PREDICTED: lysosomal Pro-X carboxypeptidase [Cucumis sativus])

HSP 1 Score: 891.7 bits (2303), Expect = 5.7e-256
Identity = 427/497 (85.92%), Postives = 460/497 (92.56%), Query Frame = 1

Query: 1   MNFPMFSS-PWLPFILLILSTCVTATQYRIPRLSPFSRTFLPNTKASP--VSDDFKTFYY 60
           M+FPMFSS PWLPFIL ILS CVTATQYRIPRLSP  RTFL N +A P  +SDDFKTFYY
Sbjct: 1   MSFPMFSSSPWLPFILFILSNCVTATQYRIPRLSPIGRTFLHNAEAIPSSISDDFKTFYY 60

Query: 61  NQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN 120
           NQTLDHFNY+PESYT FPHRYIINF YWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN
Sbjct: 61  NQTLDHFNYRPESYTCFPHRYIINFKYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN 120

Query: 121 ALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHA 180
           A +FDALLVYIEHRYYGKS+PFGSR+EALKNASTLGYF+SAQAIADYAAVLIHLK+K HA
Sbjct: 121 AARFDALLVYIEHRYYGKSMPFGSREEALKNASTLGYFSSAQAIADYAAVLIHLKQKYHA 180

Query: 181 KDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKE 240
           KD PVIVLGGSYGGMLAAWFRLKYP+VALGALASSAPILYF+DITPHNGYYSIATKDF+E
Sbjct: 181 KDSPVIVLGGSYGGMLAAWFRLKYPHVALGALASSAPILYFEDITPHNGYYSIATKDFRE 240

Query: 241 VSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYN 300
           VSE+CYETIRDSWS++E + SKPNGLSILSKEFKTCSPLNS SQLEDYLWSMYAGAAQYN
Sbjct: 241 VSETCYETIRDSWSKIEIIGSKPNGLSILSKEFKTCSPLNSSSQLEDYLWSMYAGAAQYN 300

Query: 301 QPPRYPVTRICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQKC 360
            PPRYPVTRICGGIDGAS  SG + K+AAGVFAY+G LSCYN+ P++ETETDVGWRWQ+C
Sbjct: 301 HPPRYPVTRICGGIDGASPGSGIISKVAAGVFAYKGNLSCYNIGPRSETETDVGWRWQRC 360

Query: 361 SEMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGS 420
           SEMVMP+ T NDTMFPP TFDL+SF+++C QLYGVS RPHWVTTYYGGNDIKL LQRFGS
Sbjct: 361 SEMVMPLSTTNDTMFPPITFDLKSFVDYCYQLYGVSSRPHWVTTYYGGNDIKLILQRFGS 420

Query: 421 NIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVSI 480
           NIIFSNGLRDPYSSGGVLQ+LSDSLLAVHT  GSHCLDILRANETDPQWLVKQRETEV I
Sbjct: 421 NIIFSNGLRDPYSSGGVLQNLSDSLLAVHTPKGSHCLDILRANETDPQWLVKQRETEVRI 480

Query: 481 IKEWIRKYYADLKEFKQ 495
           I+ WI KYYADL++ K+
Sbjct: 481 IEGWISKYYADLEKSKK 497

BLAST of CmaCh20G005370 vs. NCBI nr
Match: gi|659117557|ref|XP_008458664.1| (PREDICTED: lysosomal Pro-X carboxypeptidase isoform X2 [Cucumis melo])

HSP 1 Score: 793.9 bits (2049), Expect = 1.6e-226
Identity = 377/436 (86.47%), Postives = 407/436 (93.35%), Query Frame = 1

Query: 1   MNFPMFSS-PWLPFILLILSTCVTATQYRIPRLSPFSRTFLPNTKA--SPVSDDFKTFYY 60
           M+FPMFSS PW+PFIL ILS CVTATQYRIPRLSP  RTFL N +A  S +SDDFKTFYY
Sbjct: 1   MSFPMFSSSPWVPFILFILSNCVTATQYRIPRLSPIGRTFLHNAEAISSSISDDFKTFYY 60

Query: 61  NQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN 120
           NQ+LDHFNY+PESYT FPHRYIINF YWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN
Sbjct: 61  NQSLDHFNYRPESYTCFPHRYIINFKYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDN 120

Query: 121 ALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHA 180
           A++FDALLVYIEHRYYGKS+PFGSR+EALKNASTLGYF+SAQAIADYAAVL+HLK+K HA
Sbjct: 121 AVRFDALLVYIEHRYYGKSMPFGSREEALKNASTLGYFSSAQAIADYAAVLLHLKQKYHA 180

Query: 181 KDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKE 240
           KD PVIVLGGSYGGMLAAWFRLKYP+VALGALASSAPILYF+DITPHNGYYSIATKDF+E
Sbjct: 181 KDSPVIVLGGSYGGMLAAWFRLKYPHVALGALASSAPILYFEDITPHNGYYSIATKDFRE 240

Query: 241 VSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYN 300
           VSE+CYETIRDSWS++E +ASKPNGLSILSKEFKTCSPLNS SQLEDYLWSMYAGAAQYN
Sbjct: 241 VSETCYETIRDSWSKIETIASKPNGLSILSKEFKTCSPLNSSSQLEDYLWSMYAGAAQYN 300

Query: 301 QPPRYPVTRICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQKC 360
            PPRYPVTRICGGIDGAS  SG + K+AAGVFAY+G L CYN+ P+N+TETDVGWRWQ+C
Sbjct: 301 HPPRYPVTRICGGIDGASPGSGIISKVAAGVFAYKGNLPCYNIGPRNDTETDVGWRWQRC 360

Query: 361 SEMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGS 420
           SEMVMP+ T NDTMFPP TFDLRSFI++C QLYGVSPRPHWVTTYYGGNDIKL LQRFGS
Sbjct: 361 SEMVMPMSTSNDTMFPPITFDLRSFIDYCYQLYGVSPRPHWVTTYYGGNDIKLILQRFGS 420

Query: 421 NIIFSNGLRDPYSSGG 434
           NIIFSNGLRDPYSSGG
Sbjct: 421 NIIFSNGLRDPYSSGG 436

BLAST of CmaCh20G005370 vs. NCBI nr
Match: gi|659117559|ref|XP_008458665.1| (PREDICTED: lysosomal Pro-X carboxypeptidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 787.3 bits (2032), Expect = 1.5e-224
Identity = 374/498 (75.10%), Postives = 430/498 (86.35%), Query Frame = 1

Query: 1   MNFPMFSSPWLPFILLILSTCV-TATQY-RIPRLSPFSRTFLPNTKA--SPVSDDFKTFY 60
           M FPMFSSPW+PF+L +LST V T+ Q+ R PRLSP    FL +++A  S  SDDFKT+Y
Sbjct: 15  MRFPMFSSPWIPFLLFVLSTSVVTSLQHNRFPRLSPIGEKFLHHSRALYSLPSDDFKTYY 74

Query: 61  YNQTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTD 120
           YNQTLDHFNY+PESYT+F  RYIINF YWGG NSSAPI AYLGAE P++GDLN IGF+TD
Sbjct: 75  YNQTLDHFNYRPESYTTFLQRYIINFKYWGGPNSSAPIFAYLGAEAPIDGDLNFIGFLTD 134

Query: 121 NALQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLH 180
           NA+QF+ALL+YIEHRYYGKSIPF SR EAL NASTLGYFNSAQAIADYAA+LIH+KK+ H
Sbjct: 135 NAIQFNALLIYIEHRYYGKSIPFRSRDEALGNASTLGYFNSAQAIADYAAILIHVKKEFH 194

Query: 181 AKDCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFK 240
           A   PVIV+GGSYGGMLA+WFRLKYP+VALGALASSAPILYFDDITP +GYYS+ TKDF+
Sbjct: 195 ANYSPVIVIGGSYGGMLASWFRLKYPHVALGALASSAPILYFDDITPQDGYYSVVTKDFR 254

Query: 241 EVSESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQY 300
            +SE+CYETI+ SWSE++ +AS+PNGLSIL +EFKTC PL  Y +LEDYLWSMYA AAQY
Sbjct: 255 GLSETCYETIKKSWSEIKTVASQPNGLSILDQEFKTCRPLRGYFELEDYLWSMYASAAQY 314

Query: 301 NQPPRYPVTRICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQK 360
           N PP+YPVTRIC  IDG  S +GTL KIAAGVFA+ G +SCY  EP+NETETDVGWRWQ 
Sbjct: 315 NHPPKYPVTRICDAIDGTYSVNGTLSKIAAGVFAFRGSISCYINEPRNETETDVGWRWQS 374

Query: 361 CSEMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFG 420
           CSEMVMPI +D+D MFPPY FDL+S IN+CN+LYGV PRPHW TTYYGG+DI+L LQRFG
Sbjct: 375 CSEMVMPISSDDD-MFPPYPFDLQSVINYCNRLYGVPPRPHWATTYYGGHDIRLVLQRFG 434

Query: 421 SNIIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVS 480
           SNIIFSNGL+DPYS  GVL S+SDSLLAVHT NGSHCLDIL+A+ETDP+WLV QR+TEV 
Sbjct: 435 SNIIFSNGLKDPYSIAGVLHSISDSLLAVHTTNGSHCLDILKAHETDPEWLVTQRKTEVG 494

Query: 481 IIKEWIRKYYADLKEFKQ 495
           IIK WI +YYADLK++KQ
Sbjct: 495 IIKGWISEYYADLKKYKQ 511

BLAST of CmaCh20G005370 vs. NCBI nr
Match: gi|659117553|ref|XP_008458662.1| (PREDICTED: lysosomal Pro-X carboxypeptidase-like [Cucumis melo])

HSP 1 Score: 778.9 bits (2010), Expect = 5.4e-222
Identity = 374/496 (75.40%), Postives = 422/496 (85.08%), Query Frame = 1

Query: 1   MNFPMFSSPWLPFILLILSTCVTATQYRIPRLSPFSRTFLPNTKAS--PVSDDFKTFYYN 60
           M FPM SSPWLPF+LL LS  VTA Q+RIPRLSP    FL ++KA   P SDDFKTFY+N
Sbjct: 1   MRFPMCSSPWLPFLLLFLSNSVTAFQFRIPRLSPIGEKFLYHSKALELPPSDDFKTFYFN 60

Query: 61  QTLDHFNYKPESYTSFPHRYIINFNYWGGANSSAPILAYLGAEGPLEGDLNAIGFMTDNA 120
           QTLDHFNY+PESYT+FP RYIINF YWGGANSSAPILAYLG E P++  +NAIGFMTDNA
Sbjct: 61  QTLDHFNYRPESYTTFPQRYIINFKYWGGANSSAPILAYLGPEAPIDSAMNAIGFMTDNA 120

Query: 121 LQFDALLVYIEHRYYGKSIPFGSRQEALKNASTLGYFNSAQAIADYAAVLIHLKKKLHAK 180
           ++F+ALLVYIEHRYYGKSIPFGSR+EAL+NASTLGYFNSAQAIADYAA+LIH+K + +AK
Sbjct: 121 VKFNALLVYIEHRYYGKSIPFGSRKEALRNASTLGYFNSAQAIADYAAILIHVKNEFNAK 180

Query: 181 DCPVIVLGGSYGGMLAAWFRLKYPNVALGALASSAPILYFDDITPHNGYYSIATKDFKEV 240
             PVIV+GGSYGGMLA WFRLKYP+VALGALASSAPILYF+DITP NGYY   TKDF+EV
Sbjct: 181 YSPVIVIGGSYGGMLATWFRLKYPHVALGALASSAPILYFNDITPQNGYYVTVTKDFREV 240

Query: 241 SESCYETIRDSWSEMEAMASKPNGLSILSKEFKTCSPLNSYSQLEDYLWSMYAGAAQYNQ 300
           S++CYETIR+SWSE+E +AS+PNGLS+L KEFKTCSPL S +QLE+YLW MYA AAQYN 
Sbjct: 241 SQTCYETIRESWSEIETVASQPNGLSVLDKEFKTCSPLRSSTQLENYLWFMYASAAQYNH 300

Query: 301 PPRYPVTRICGGIDGASSESGTLGKIAAGVFAYEGKLSCYNLEPKNETETDVGWRWQKCS 360
           P  YPVTRIC  ID   S +GTLGKIAAGVFAY G LSCY  EP N TET VGW+WQ+CS
Sbjct: 301 PSSYPVTRICDAIDRTYS-NGTLGKIAAGVFAYRGNLSCYINEPINTTETTVGWQWQRCS 360

Query: 361 EMVMPIGTDNDTMFPPYTFDLRSFINHCNQLYGVSPRPHWVTTYYGGNDIKLHLQRFGSN 420
           EMVMPI T NDTMFPP TFD  SF  +CNQLYGV+PRPHWVTTYYGG+D+ L L RF SN
Sbjct: 361 EMVMPISTSNDTMFPPRTFDHESFSIYCNQLYGVTPRPHWVTTYYGGDDVHLILHRFASN 420

Query: 421 IIFSNGLRDPYSSGGVLQSLSDSLLAVHTANGSHCLDILRANETDPQWLVKQRETEVSII 480
           IIFSNGL+DPYS GGVL ++SDSL AV+TANGSHCLDIL +N  DP+WLV QR+TEV II
Sbjct: 421 IIFSNGLKDPYSIGGVLHNISDSLPAVYTANGSHCLDILSSNRMDPEWLVTQRKTEVRII 480

Query: 481 KEWIRKYYADLKEFKQ 495
           KEWI KYYADL  +K+
Sbjct: 481 KEWIDKYYADLANYKK 495

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PCP_MOUSE5.6e-8636.58Lysosomal Pro-X carboxypeptidase OS=Mus musculus GN=Prcp PE=1 SV=2[more]
PCP_PONAB4.7e-8536.84Lysosomal Pro-X carboxypeptidase OS=Pongo abelii GN=PRCP PE=2 SV=1[more]
PCP_HUMAN1.8e-8436.84Lysosomal Pro-X carboxypeptidase OS=Homo sapiens GN=PRCP PE=1 SV=1[more]
PCP_BOVIN1.1e-8136.83Lysosomal Pro-X carboxypeptidase OS=Bos taurus GN=PRCP PE=2 SV=1[more]
DPP2_RAT7.1e-6532.98Dipeptidyl peptidase 2 OS=Rattus norvegicus GN=Dpp7 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KAY8_CUCSA4.0e-25685.92Uncharacterized protein OS=Cucumis sativus GN=Csa_6G149410 PE=4 SV=1[more]
A0A0A0KBK9_CUCSA5.4e-22173.90Uncharacterized protein OS=Cucumis sativus GN=Csa_6G149390 PE=4 SV=1[more]
A0A0A0KDH5_CUCSA3.0e-21975.20Uncharacterized protein OS=Cucumis sativus GN=Csa_6G149400 PE=4 SV=1[more]
B9HQR7_POPTR5.5e-19766.94Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s00740g PE=4 SV=2[more]
A0A061GWT8_THECC1.3e-19566.80Serine carboxypeptidase S28 family protein OS=Theobroma cacao GN=TCM_046935 PE=4... [more]
Match NameE-valueIdentityDescription
AT5G22860.11.3e-15754.55 Serine carboxypeptidase S28 family protein[more]
AT2G24280.12.8e-12046.46 alpha/beta-Hydrolases superfamily protein[more]
AT5G65760.16.5e-11746.59 Serine carboxypeptidase S28 family protein[more]
AT3G28680.11.1e-3947.40 Serine carboxypeptidase S28 family protein[more]
AT4G36195.12.6e-2527.59 Serine carboxypeptidase S28 family protein[more]
Match NameE-valueIdentityDescription
gi|659117555|ref|XP_008458663.1|3.3e-25685.71PREDICTED: lysosomal Pro-X carboxypeptidase isoform X1 [Cucumis melo][more]
gi|449456064|ref|XP_004145770.1|5.7e-25685.92PREDICTED: lysosomal Pro-X carboxypeptidase [Cucumis sativus][more]
gi|659117557|ref|XP_008458664.1|1.6e-22686.47PREDICTED: lysosomal Pro-X carboxypeptidase isoform X2 [Cucumis melo][more]
gi|659117559|ref|XP_008458665.1|1.5e-22475.10PREDICTED: lysosomal Pro-X carboxypeptidase-like isoform X1 [Cucumis melo][more]
gi|659117553|ref|XP_008458662.1|5.4e-22275.40PREDICTED: lysosomal Pro-X carboxypeptidase-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008758Peptidase_S28
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0008236serine-type peptidase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004180 carboxypeptidase activity
molecular_function GO:0008236 serine-type peptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G005370.1CmaCh20G005370.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008758Peptidase S28PFAMPF05577Peptidase_S28coord: 59..472
score: 2.4
NoneNo IPR availableunknownCoilCoilcoord: 482..494
scor
NoneNo IPR availablePANTHERPTHR11010PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATEDcoord: 1..490
score: 6.6E
NoneNo IPR availablePANTHERPTHR11010:SF47PROLYLCARBOXYPEPTIDASE-LIKE PROTEIN-RELATEDcoord: 1..490
score: 6.6E

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh20G005370Cucumber (Chinese Long) v2cmacuB547
CmaCh20G005370Cucumber (Chinese Long) v2cmacuB561
CmaCh20G005370Melon (DHL92) v3.5.1cmameB520
CmaCh20G005370Watermelon (Charleston Gray)cmawcgB476
CmaCh20G005370Watermelon (Charleston Gray)cmawcgB506
CmaCh20G005370Watermelon (97103) v1cmawmB513
CmaCh20G005370Watermelon (97103) v1cmawmB523
CmaCh20G005370Cucurbita pepo (Zucchini)cmacpeB564
CmaCh20G005370Cucurbita pepo (Zucchini)cmacpeB594
CmaCh20G005370Bottle gourd (USVL1VR-Ls)cmalsiB506
CmaCh20G005370Bottle gourd (USVL1VR-Ls)cmalsiB518
CmaCh20G005370Bottle gourd (USVL1VR-Ls)cmalsiB524
CmaCh20G005370Cucumber (Gy14) v2cgybcmaB388
CmaCh20G005370Cucumber (Gy14) v2cgybcmaB790
CmaCh20G005370Cucumber (Gy14) v2cgybcmaB795
CmaCh20G005370Silver-seed gourdcarcmaB0473
CmaCh20G005370Silver-seed gourdcarcmaB1266
CmaCh20G005370Cucumber (Chinese Long) v3cmacucB0656
CmaCh20G005370Cucumber (Chinese Long) v3cmacucB0672
CmaCh20G005370Watermelon (97103) v2cmawmbB565
CmaCh20G005370Watermelon (97103) v2cmawmbB594
CmaCh20G005370Wax gourdcmawgoB0697
CmaCh20G005370Cucurbita maxima (Rimu)cmacmaB261
CmaCh20G005370Cucurbita maxima (Rimu)cmacmaB479
CmaCh20G005370Cucurbita maxima (Rimu)cmacmaB490
CmaCh20G005370Cucurbita maxima (Rimu)cmacmaB493
CmaCh20G005370Cucumber (Gy14) v1cgycmaB1065
CmaCh20G005370Cucurbita moschata (Rifu)cmacmoB544
CmaCh20G005370Cucurbita moschata (Rifu)cmacmoB550
CmaCh20G005370Cucurbita moschata (Rifu)cmacmoB563
CmaCh20G005370Cucurbita moschata (Rifu)cmacmoB569
CmaCh20G005370Wild cucumber (PI 183967)cmacpiB551