CmaCh19G003540 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh19G003540
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Polygalacturonase
Location	Cma_Chr19 : 3823912 .. 3824868 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTTTTCATTACTGCGGTATTATTGATGGGCAAGGAACTCCCTTGTTGAATTGTAAACACTCAGGGAAGAGTTGCCCCATTGGAGCCTGGGTATGCAGAATTTGAGTTGGCTCCATATAAAAAAAATTTGAAAATTTTTCGTTAACTGAAAGTTGAATGAAAATGACCCGATTAATTACAATATTATTTTAAATTTGTACTAATTCTCACTTTTGATTGTTTTGAATTGATAGAGTCTTCAAATAAATGATGGAAAGAACGTGAACATCAATGGATTATCTTCAATTAACAGCCAATTGTTTAGCATTGCAATGAATCGGTGCCAAAACGTGCATATGACAAGGGTGAATGTCTTGGCTGCAGGTGATAGTCCAAACACTGATGGCATCCATGTGCAACAATCATCAGATGTGACCATCCTCAACTTGAACATTGGAAACGGTGACGACTGCATCTCCATCGGTCCAGGGACATCCAACATGTGGATGGAAAGTATTACATGTGGACCTGGACATGGAATCAGGTAACTAGAGATAGATTAGGATTAACATGTTTAGAAATGGTTTTTAAAGGGTTTAAATCAATTTGTCACATTAATTTTTAAAAGATAATCTAAACCTTAAATAAATTTGAATGATTAAAATATATTCTAAAGTGGTTTGAAAGGGGAGAAAAATGATTTAAATTATTTTAAAATTACTTTGGATAAAAACATTTATATAATATATTATGTTGCGAGTGAGGAAATAAAGGTCAGGAAACATTTGGTATTTTGTTGAATGTGTTTGTAGCATTGAGAGCTTAGGAATAAAGGCTGAGGAGGATGGAGTGCATAACGTTACCGTGAAATCAACTACATTCACAGGGATTCAGAATGGAGTAAGAATTAAGTCTTGGGGAAGACCAAGCAATGGATTCGCTACATTCATATTCAACACATTATTATGGATAATGTGA

mRNA sequence

GCTTTTCATTACTGCGGTATTATTGATGGGCAAGGAACTCCCTTGTTGAATTGTAAACACTCAGGGAAGAGTTGCCCCATTGGAGCCTGGAGTCTTCAAATAAATGATGGAAAGAACGTGAACATCAATGGATTATCTTCAATTAACAGCCAATTGTTTAGCATTGCAATGAATCGGTGCCAAAACGTGCATATGACAAGGGTGAATGTCTTGGCTGCAGGTGATAGTCCAAACACTGATGGCATCCATGTGCAACAATCATCAGATGTGACCATCCTCAACTTGAACATTGGAAACGGTGACGACTGCATCTCCATCGGTCCAGGGACATCCAACATGTGGATGGAAAGTATTACATGTGGACCTGGACATGGAATCAGCTTAGGAATAAAGGCTGAGGAGGATGGAGTGCATAACGTTACCGTGAAATCAACTACATTCACAGGGATTCAGAATGGAGTAAGAATTAAGTCTTGGGGAAGACCAAGCAATGGATTCGCTACATTCATATTCAACACATTATTATGGATAATGTGA

Coding sequence (CDS)

GCTTTTCATTACTGCGGTATTATTGATGGGCAAGGAACTCCCTTGTTGAATTGTAAACACTCAGGGAAGAGTTGCCCCATTGGAGCCTGGAGTCTTCAAATAAATGATGGAAAGAACGTGAACATCAATGGATTATCTTCAATTAACAGCCAATTGTTTAGCATTGCAATGAATCGGTGCCAAAACGTGCATATGACAAGGGTGAATGTCTTGGCTGCAGGTGATAGTCCAAACACTGATGGCATCCATGTGCAACAATCATCAGATGTGACCATCCTCAACTTGAACATTGGAAACGGTGACGACTGCATCTCCATCGGTCCAGGGACATCCAACATGTGGATGGAAAGTATTACATGTGGACCTGGACATGGAATCAGCTTAGGAATAAAGGCTGAGGAGGATGGAGTGCATAACGTTACCGTGAAATCAACTACATTCACAGGGATTCAGAATGGAGTAAGAATTAAGTCTTGGGGAAGACCAAGCAATGGATTCGCTACATTCATATTCAACACATTATTATGGATAATGTGA

Protein sequence

AFHYCGIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVHMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGHGISLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFATFIFNTLLWIM

Homology

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BLAST of CmaCh19G003540 vs. Swiss-Prot
Match: PGLR_PRUPE (Polygalacturonase OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 5.9e-54
Identity = 104/166 (62.65%), Postives = 125/166 (75.30%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHS-GKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVH 65
           GI+DGQGT L  CK   G+SCP GA +L  +D  N+ ++GL+S+NSQ+F I +N  QNV 
Sbjct: 123 GILDGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQ 182

Query: 66  MTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGH 125
           M  V V  +G+SPNTDGIHVQ SS VTILN  I  GDDC+SIGPGTSN+W+E + CGPGH
Sbjct: 183 MQGVRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGH 242

Query: 126 GI---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           GI   SLG + EE GV NVTVK+ TF+G QNG+RIKSWGRPS GFA
Sbjct: 243 GISIGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFA 288

[Close]

BLAST of CmaCh19G003540 vs. Swiss-Prot
Match: PGLR6_ARATH (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.5e-44
Identity = 92/168 (54.76%), Postives = 116/168 (69.05%), Query Frame = 1


Query: 4   YCGIIDGQGTPLLNCKHSG-KSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQN 63
           Y GI+D QGT L  CK++G  +CP GA SL  +   N+ I+GL+SINSQ F I ++   N
Sbjct: 127 YGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNN 186

Query: 64  VHMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGP 123
           V++  V V A  +SPNTDGIHV+ S  V I N  IG GDDCISIGPG++N+++++I CGP
Sbjct: 187 VNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGP 246

Query: 124 GHGI---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           GHGI   SLG   EE GV NVTV +  F G  NGVRIK+WG+ SN FA
Sbjct: 247 GHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFA 294

[Close]

BLAST of CmaCh19G003540 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 9.2e-31
Identity = 70/157 (44.59%), Postives = 95/157 (60.51%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVHM 65
           GI+DGQ      CK S K C     +L  N   N  I  +++++S+ F + +N+C+N+  
Sbjct: 119 GILDGQAAAAWECKQS-KKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTF 178

Query: 66  TRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGHG 125
            R NV A  +SPNTDGIHV +SS V I + N   GDDCIS+G  T  +++  +TCGPGHG
Sbjct: 179 IRFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHG 238

Query: 126 I---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSW 160
           I   SLG   +E  V  V V++ TFT   NGVRIK+W
Sbjct: 239 ISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTW 274

[Close]

BLAST of CmaCh19G003540 vs. Swiss-Prot
Match: PGLR2_PLAAC (Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.6e-30
Identity = 72/169 (42.60%), Postives = 98/169 (57.99%), Query Frame = 1


Query: 6   GIIDGQGTPLL---NCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQN 65
           G +DGQG       NC  +  +C   A +L+ +  K+  +  ++S+NS++F I +  C++
Sbjct: 103 GTLDGQGQTAWAKNNCDKN-PNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECED 162

Query: 66  VHMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGP 125
           +    V V A G S NTDGIHV  S  VTI N  I  GDDCISIGPG+ N+ +  + CGP
Sbjct: 163 ITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGP 222

Query: 126 GHGISLGIKA---EEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFAT 169
           GHGIS+G       E  V  +TVK  TF+G  NGVR+K+W     G AT
Sbjct: 223 GHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPNSPPGAAT 270

[Close]

BLAST of CmaCh19G003540 vs. Swiss-Prot
Match: PGLR_MEDSA (Polygalacturonase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.1e-30
Identity = 68/168 (40.48%), Postives = 100/168 (59.52%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGK-----------NVNINGLSSINSQLFS 65
           G+ DGQG  +       K+ P  AWS + ++ K           N  + G++S +S+ F 
Sbjct: 118 GVFDGQGATVYK-----KAAPASAWSGKNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFH 177

Query: 66  IAMNRCQNVHMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMW 125
           + +  C+N+      + A GDSPNTDGIH+ +S+DV ILN NIG GDDC+SIG G+  + 
Sbjct: 178 VMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQIT 237

Query: 126 MESITCGPGHGISLGIKAE---EDGVHNVTVKSTTFTGIQNGVRIKSW 160
           ++ + CGPGHG+S+G   +   E+ V  +TVK+ T T   NGVRIK+W
Sbjct: 238 VQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTW 280

[Close]

BLAST of CmaCh19G003540 vs. TrEMBL
Match: A0A0A0KN91_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G409610 PE=3 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 5.0e-60
Identity = 116/170 (68.24%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 4   YCGIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNV 63
           Y G+IDGQG  L NCKHSGKSCP GA +L+I+  +NVN+NGLSSINSQ+F+I +  C+NV
Sbjct: 120 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENV 179

Query: 64  HMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPG 123
            +  VNV +AGDSPNTDGIHVQQS +V I + +IG GDDCISIGPGT+N+WME+I CGPG
Sbjct: 180 QIQGVNVSSAGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPG 239

Query: 124 HGI---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFATFI 171
           HGI   SLG + EE GV NVTV + TFTG QNGVRIKSWGRPSN FA  I
Sbjct: 240 HGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKI 289

[Close]

BLAST of CmaCh19G003540 vs. TrEMBL
Match: G7JIR7_MEDTR (Polygalacturonase OS=Medicago truncatula GN=MTR_4g083660 PE=3 SV=2)

HSP 1 Score: 231.9 bits (590), Expect = 6.2e-58
Identity = 113/170 (66.47%), Postives = 135/170 (79.41%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKS-CPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVH 65
           G++DGQGT L +CK+S KS CPIGA +L+ ++ KN+ I+GL+S+NSQ+F I  N C+NV 
Sbjct: 104 GLLDGQGTALWDCKNSDKSNCPIGATTLEFSNSKNIMISGLTSMNSQMFHIVFNGCENVK 163

Query: 66  MTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGH 125
           M  VN++AAG+SPNTDGIHVQ SS VTI+N  I  GDDCISIGPGTSN+W+E+I CGPGH
Sbjct: 164 MQGVNIMAAGNSPNTDGIHVQMSSHVTIVNSKIRTGDDCISIGPGTSNLWIENIACGPGH 223

Query: 126 GI---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFATFIF 172
           GI   SLG K  E GV NVTVK+ TFTG QNGVRIKSWGRPSNGF   IF
Sbjct: 224 GISIGSLGWKLNEPGVQNVTVKTVTFTGTQNGVRIKSWGRPSNGFVRNIF 273

[Close]

BLAST of CmaCh19G003540 vs. TrEMBL
Match: B9HGC5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s00510g PE=3 SV=2)

HSP 1 Score: 230.3 bits (586), Expect = 1.8e-57
Identity = 108/165 (65.45%), Postives = 131/165 (79.39%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVHM 65
           G +DGQG  L +CK+SGK CP GA SL+ ++  N+ I GL+S+NSQ+F I +N CQNV +
Sbjct: 127 GTLDGQGAGLWSCKNSGKDCPSGATSLEFSNSNNIAITGLASLNSQMFHIVINACQNVKV 186

Query: 66  TRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGHG 125
             V V AAGDSPNTDGIHVQ S+ VTILN  IG GDDC+SIGPGTSN+W+E++ CGPGHG
Sbjct: 187 QGVRVSAAGDSPNTDGIHVQSSTGVTILNSRIGTGDDCVSIGPGTSNLWIENVACGPGHG 246

Query: 126 I---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           I   SLG  ++E GV NVTVK+TTFTG +NGVRIK+WGRPSNGFA
Sbjct: 247 ISIGSLGKDSQEAGVQNVTVKTTTFTGTENGVRIKTWGRPSNGFA 291

[Close]

BLAST of CmaCh19G003540 vs. TrEMBL
Match: U5G4M7_POPTR (Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0008s01120g PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 8.9e-57
Identity = 108/165 (65.45%), Postives = 129/165 (78.18%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVHM 65
           G +DGQG  L +CK SGKSCP GA SL+ ++  N+ I GL+S+NSQ+F I +N CQNV +
Sbjct: 100 GTLDGQGAGLWSCKKSGKSCPSGATSLEFSNSNNIVITGLTSLNSQMFHIVINACQNVKV 159

Query: 66  TRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGHG 125
             V V AAG+SPNTDGIHV  S+ VTILN  IG GDDC+SIGPGTSN+W+E++ CGPGHG
Sbjct: 160 KGVRVSAAGNSPNTDGIHVSSSTSVTILNSRIGTGDDCVSIGPGTSNLWIENVACGPGHG 219

Query: 126 I---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           I   SLG   +E GV NVTVK+TTFTG +NGVRIKSWGRPSNGFA
Sbjct: 220 ISIGSLGQDVQEAGVQNVTVKTTTFTGTENGVRIKSWGRPSNGFA 264

[Close]

BLAST of CmaCh19G003540 vs. TrEMBL
Match: U5FKL6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s03850g PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.5e-56
Identity = 107/165 (64.85%), Postives = 132/165 (80.00%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVHM 65
           G +DGQG  L +CK+SGK CP GA SL+ ++  N+ I GL+S+NSQLF I +N CQNV +
Sbjct: 128 GTLDGQGAGLWSCKNSGKGCPRGATSLEFSNSNNIAITGLASLNSQLFHIVINGCQNVKV 187

Query: 66  TRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGHG 125
             V V AAG+SPNTDGIHVQ S+ VTILN  IG GDDC+SIGPGTS++W+E++ CGPGHG
Sbjct: 188 QGVKVSAAGNSPNTDGIHVQSSTGVTILNSRIGTGDDCVSIGPGTSSLWIENVACGPGHG 247

Query: 126 I---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           I   SLG +++E GV NVTVK+TTFTG +NG+RIKSWGRPSNGFA
Sbjct: 248 ISIGSLGKESQEAGVQNVTVKTTTFTGTENGLRIKSWGRPSNGFA 292

[Close]

BLAST of CmaCh19G003540 vs. TAIR10
Match: AT3G59850.1 (AT3G59850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 213.4 bits (542), Expect = 1.1e-55
Identity = 99/167 (59.28%), Postives = 125/167 (74.85%), Query Frame = 1


Query: 4   YCGIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNV 63
           Y G++D QG  L +CK SGK+CP GA S+     +NV I+GL+S+NSQ+F +A+N C NV
Sbjct: 117 YGGVLDAQGASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNV 176

Query: 64  HMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPG 123
            +  V V A G+SPNTDGIHVQ SS V+ILN  I  GDDC+SIGPGT+ +W+E++ CGPG
Sbjct: 177 KLDGVKVSADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPG 236

Query: 124 HGISLGIKAEED---GVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           HGIS+G   +E    GV N+TVK+ TFTG +NGVRIKSW RPSNGFA
Sbjct: 237 HGISIGSLGKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFA 283

[Close]

BLAST of CmaCh19G003540 vs. TAIR10
Match: AT2G43870.1 (AT2G43870.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 209.5 bits (532), Expect = 1.7e-54
Identity = 97/167 (58.08%), Postives = 123/167 (73.65%), Query Frame = 1


Query: 4   YCGIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNV 63
           Y G++D +G  L +CK SGK+CP GA ++      NV ++GL+S+NSQ+F + +N C NV
Sbjct: 115 YGGVLDARGASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNV 174

Query: 64  HMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPG 123
            +  V VLAAG+SPNTDGIHVQ SS V+I N  I  GDDC+SIGPGT+ +W+E++ CGPG
Sbjct: 175 KLQGVKVLAAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPG 234

Query: 124 HGI---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           HGI   SLG  + E GV NVTVK+ TFTG  NGVRIKSW RPS+GFA
Sbjct: 235 HGISIGSLGKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFA 281

[Close]

BLAST of CmaCh19G003540 vs. TAIR10
Match: AT1G65570.1 (AT1G65570.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 189.9 bits (481), Expect = 1.4e-48
Identity = 90/164 (54.88%), Postives = 117/164 (71.34%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVHM 65
           G  D +G+ L +CK +G +CP GA +L+  D  NV I G+ S+NSQLF IA+NRC+N+ +
Sbjct: 128 GSFDAKGSTLWSCKANGYNCPEGATTLRFMDSNNVKIKGVLSLNSQLFHIAINRCRNIKI 187

Query: 66  TRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGHG 125
             V ++A  +SPNTDGIH+Q S+D+ + N +I  GDDCISIGPGT N+ ++ ITCGPGHG
Sbjct: 188 EDVRIIAPDESPNTDGIHIQLSTDIEVRNASIKTGDDCISIGPGTKNLMVDGITCGPGHG 247

Query: 126 ISLGIKA---EEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGF 167
           IS+G  A   EE GV NVTVK+  F    NG+RIKSW R SNGF
Sbjct: 248 ISIGSLAKSIEEQGVENVTVKNAVFVRTDNGLRIKSWPRHSNGF 291

[Close]

BLAST of CmaCh19G003540 vs. TAIR10
Match: AT1G05660.1 (AT1G05660.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 180.6 bits (457), Expect = 8.3e-46
Identity = 84/169 (49.70%), Postives = 113/169 (66.86%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVHM 65
           G  D +     +C+ SG++CP G  S+  N  K+V I+G+ S+NSQ+  + +N C NV +
Sbjct: 122 GTFDARANGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVV 181

Query: 66  TRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGHG 125
             V ++A G+SPNTDG HVQ S+ VT     +  GDDC++IGPGT N+ +  + CGPGHG
Sbjct: 182 RNVKLVAPGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHG 241

Query: 126 ISLGIKA---EEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFATFIF 172
           +S+G  A   +EDGV NVTV S+ FTG QNGVRIKSW RPSNGF   +F
Sbjct: 242 VSIGSLAKELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVF 290

[Close]

BLAST of CmaCh19G003540 vs. TAIR10
Match: AT2G43860.1 (AT2G43860.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 180.6 bits (457), Expect = 8.3e-46
Identity = 92/168 (54.76%), Postives = 116/168 (69.05%), Query Frame = 1


Query: 4   YCGIIDGQGTPLLNCKHSG-KSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQN 63
           Y GI+D QGT L  CK++G  +CP GA SL  +   N+ I+GL+SINSQ F I ++   N
Sbjct: 127 YGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNN 186

Query: 64  VHMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGP 123
           V++  V V A  +SPNTDGIHV+ S  V I N  IG GDDCISIGPG++N+++++I CGP
Sbjct: 187 VNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGP 246

Query: 124 GHGI---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           GHGI   SLG   EE GV NVTV +  F G  NGVRIK+WG+ SN FA
Sbjct: 247 GHGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFA 294

[Close]

BLAST of CmaCh19G003540 vs. NCBI nr
Match: gi|659121214|ref|XP_008460550.1| (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 243.4 bits (620), Expect = 2.9e-61
Identity = 119/170 (70.00%), Postives = 137/170 (80.59%), Query Frame = 1


Query: 4   YCGIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNV 63
           Y G+IDGQG  L NCKHSG+SCP GA SL+I D +NVN+NGLSS+NSQ+F+I +  CQNV
Sbjct: 120 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 179

Query: 64  HMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPG 123
            +  VNV +A DSPNTDGIHVQQS +V I N NIG GDDCISIGPGT+N+WME+I CGPG
Sbjct: 180 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 239

Query: 124 HGI---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFATFI 171
           HGI   SLG +AEE GV NVTV + TF G QNGVRIKSWGRPS+GFAT I
Sbjct: 240 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKI 289

[Close]

BLAST of CmaCh19G003540 vs. NCBI nr
Match: gi|449445220|ref|XP_004140371.1| (PREDICTED: polygalacturonase [Cucumis sativus])

HSP 1 Score: 238.8 bits (608), Expect = 7.2e-60
Identity = 116/170 (68.24%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 4   YCGIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNV 63
           Y G+IDGQG  L NCKHSGKSCP GA +L+I+  +NVN+NGLSSINSQ+F+I +  C+NV
Sbjct: 120 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENV 179

Query: 64  HMTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPG 123
            +  VNV +AGDSPNTDGIHVQQS +V I + +IG GDDCISIGPGT+N+WME+I CGPG
Sbjct: 180 QIQGVNVSSAGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPG 239

Query: 124 HGI---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFATFI 171
           HGI   SLG + EE GV NVTV + TFTG QNGVRIKSWGRPSN FA  I
Sbjct: 240 HGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKI 289

[Close]

BLAST of CmaCh19G003540 vs. NCBI nr
Match: gi|922363278|ref|XP_003607860.2| (polygalacturonase [Medicago truncatula])

HSP 1 Score: 231.9 bits (590), Expect = 8.9e-58
Identity = 113/170 (66.47%), Postives = 135/170 (79.41%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKS-CPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVH 65
           G++DGQGT L +CK+S KS CPIGA +L+ ++ KN+ I+GL+S+NSQ+F I  N C+NV 
Sbjct: 104 GLLDGQGTALWDCKNSDKSNCPIGATTLEFSNSKNIMISGLTSMNSQMFHIVFNGCENVK 163

Query: 66  MTRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGH 125
           M  VN++AAG+SPNTDGIHVQ SS VTI+N  I  GDDCISIGPGTSN+W+E+I CGPGH
Sbjct: 164 MQGVNIMAAGNSPNTDGIHVQMSSHVTIVNSKIRTGDDCISIGPGTSNLWIENIACGPGH 223

Query: 126 GI---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFATFIF 172
           GI   SLG K  E GV NVTVK+ TFTG QNGVRIKSWGRPSNGF   IF
Sbjct: 224 GISIGSLGWKLNEPGVQNVTVKTVTFTGTQNGVRIKSWGRPSNGFVRNIF 273

[Close]

BLAST of CmaCh19G003540 vs. NCBI nr
Match: gi|566178903|ref|XP_002310385.2| (hypothetical protein POPTR_0007s00510g [Populus trichocarpa])

HSP 1 Score: 230.3 bits (586), Expect = 2.6e-57
Identity = 108/165 (65.45%), Postives = 131/165 (79.39%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVHM 65
           G +DGQG  L +CK+SGK CP GA SL+ ++  N+ I GL+S+NSQ+F I +N CQNV +
Sbjct: 127 GTLDGQGAGLWSCKNSGKDCPSGATSLEFSNSNNIAITGLASLNSQMFHIVINACQNVKV 186

Query: 66  TRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGHG 125
             V V AAGDSPNTDGIHVQ S+ VTILN  IG GDDC+SIGPGTSN+W+E++ CGPGHG
Sbjct: 187 QGVRVSAAGDSPNTDGIHVQSSTGVTILNSRIGTGDDCVSIGPGTSNLWIENVACGPGHG 246

Query: 126 I---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           I   SLG  ++E GV NVTVK+TTFTG +NGVRIK+WGRPSNGFA
Sbjct: 247 ISIGSLGKDSQEAGVQNVTVKTTTFTGTENGVRIKTWGRPSNGFA 291

[Close]

BLAST of CmaCh19G003540 vs. NCBI nr
Match: gi|743922387|ref|XP_011005268.1| (PREDICTED: polygalacturonase-like isoform X1 [Populus euphratica])

HSP 1 Score: 228.4 bits (581), Expect = 9.8e-57
Identity = 107/165 (64.85%), Postives = 132/165 (80.00%), Query Frame = 1


Query: 6   GIIDGQGTPLLNCKHSGKSCPIGAWSLQINDGKNVNINGLSSINSQLFSIAMNRCQNVHM 65
           G +DGQG  L +CK+SGK+CP GA SL+ ++  N+ I GL+S+NSQ+F I +N CQ+V +
Sbjct: 122 GTLDGQGAGLWSCKNSGKNCPRGATSLEFSNSNNIAITGLASLNSQMFHIVINACQDVKV 181

Query: 66  TRVNVLAAGDSPNTDGIHVQQSSDVTILNLNIGNGDDCISIGPGTSNMWMESITCGPGHG 125
             V V AAGDSPNTDGIHVQ S+ VTILN  IG GDDC+SIGPGTSN+W+E++ CGPGHG
Sbjct: 182 QGVRVSAAGDSPNTDGIHVQSSTGVTILNSRIGTGDDCVSIGPGTSNLWIENVACGPGHG 241

Query: 126 I---SLGIKAEEDGVHNVTVKSTTFTGIQNGVRIKSWGRPSNGFA 168
           I   SLG  ++E GV NVTVK+TTFTG +NGVRIK+WGRPSNGFA
Sbjct: 242 ISIGSLGKDSQEAGVQNVTVKTTTFTGTENGVRIKTWGRPSNGFA 286

The following BLAST results are available for this feature:

BLAST of CmaCh19G003540 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGLR_PRUPE	5.9e-54	62.65	Polygalacturonase OS=Prunus persica PE=2 SV=1	[more]
PGLR6_ARATH	1.5e-44	54.76	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 S...	[more]
PGLR_TOBAC	9.2e-31	44.59	Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1	[more]
PGLR2_PLAAC	1.6e-30	42.60	Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1	[more]
PGLR_MEDSA	2.1e-30	40.48	Polygalacturonase OS=Medicago sativa PE=2 SV=1	[more]

BLAST of CmaCh19G003540 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KN91_CUCSA	5.0e-60	68.24	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G409610 PE=3 SV=1	[more]
G7JIR7_MEDTR	6.2e-58	66.47	Polygalacturonase OS=Medicago truncatula GN=MTR_4g083660 PE=3 SV=2	[more]
B9HGC5_POPTR	1.8e-57	65.45	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s00510g PE=3 SV=2	[more]
U5G4M7_POPTR	8.9e-57	65.45	Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0008s01120g P...	[more]
U5FKL6_POPTR	1.5e-56	64.85	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s03850g PE=3 SV=1	[more]

BLAST of CmaCh19G003540 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G59850.1	1.1e-55	59.28	Pectin lyase-like superfamily protein	[more]
AT2G43870.1	1.7e-54	58.08	Pectin lyase-like superfamily protein	[more]
AT1G65570.1	1.4e-48	54.88	Pectin lyase-like superfamily protein	[more]
AT1G05660.1	8.3e-46	49.70	Pectin lyase-like superfamily protein	[more]
AT2G43860.1	8.3e-46	54.76	Pectin lyase-like superfamily protein	[more]

BLAST of CmaCh19G003540 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659121214\|ref\|XP_008460550.1\|	2.9e-61	70.00	PREDICTED: polygalacturonase-like [Cucumis melo]	[more]
gi\|449445220\|ref\|XP_004140371.1\|	7.2e-60	68.24	PREDICTED: polygalacturonase [Cucumis sativus]	[more]
gi\|922363278\|ref\|XP_003607860.2\|	8.9e-58	66.47	polygalacturonase [Medicago truncatula]	[more]
gi\|566178903\|ref\|XP_002310385.2\|	2.6e-57	65.45	hypothetical protein POPTR_0007s00510g [Populus trichocarpa]	[more]
gi\|743922387\|ref\|XP_011005268.1\|	9.8e-57	64.85	PREDICTED: polygalacturonase-like isoform X1 [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000743	Glyco_hydro_28
IPR006626	PbH1
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071555	cell wall organization
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh19G003540.1	CmaCh19G003540.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 6..160 score: 2.7
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 137..158 score: 27.0coord: 87..108 score: 410.0coord: 60..86 score: 3
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 6..167 score: 6.58
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 6..167 score: 2.7
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 6..170 score: 2.1
None	No IPR available	PANTHER	PTHR31375:SF2	POLYGALACTURONASE/PECTINASE	coord: 6..170 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh19G003540	CmoCh19G003880	Cucurbita moschata (Rifu)	cmacmoB512

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh19G003540	Watermelon (97103) v2	cmawmbB560
CmaCh19G003540	Cucurbita moschata (Rifu)	cmacmoB508
CmaCh19G003540	Cucurbita moschata (Rifu)	cmacmoB510
CmaCh19G003540	Cucurbita moschata (Rifu)	cmacmoB513
CmaCh19G003540	Cucurbita pepo (Zucchini)	cmacpeB522