CmaCh19G003170 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh19G003170
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPolygalacturonase family protein
LocationCma_Chr19 : 2801502 .. 2801935 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTCTATGGAACCTTAATGCTTCTTCTTTCCTTTCTGTTACTTTTGCTTTTGAACCATGCCTTGGCTGATGGATTAACCTTAGATATCGTTAGTCTTGGAGCCAAGCCCGATGGAAAAATCGATGCCTCGCTTGTACTTCAAATTTGTCTTTGGGGGAAAGTTGAAAGAAAATGCATGGTACTCATTCTTACTCTTGGATTGTTTTGAATTTGTAGAATCTGGAAATCACAAATGCACAAAATGTTATCATCAATGGATTAGCTTCAATTAATAGCCAAATATTTAACATTATGGTGCACGGTTGCCAAAATGTGAAAATTCAAGGAGTGAAGGTCTCGGATCCTGACGATAGTTCGAACACTGATGGCTTCCATGTGCAGGAGTCCATCAGATGTGACCATTCTCAGTTCCAACATTGGCACCGGTGA

mRNA sequence

ATGGCTCTCTATGGAACCTTAATGCTTCTTCTTTCCTTTCTGTTACTTTTGCTTTTGAACCATGCCTTGGCTGATGGATTAACCTTAGATATCGTTAGTCTTGGAGCCAAGCCCGATGGAAAAATCGATGCCTCGCTTAATCTGGAAATCACAAATGCACAAAATGTTATCATCAATGGATTAGCTTCAATTAATAGCCAAATATTTAACATTATGGTGCACGGTTGCCAAAATGTGAAAATTCAAGGAGTGAAGGTCTCGGATCCTGACGATAGTTCGAACACTGATGGCTTCCATGTGCAGGAGTCCATCAGATGTGACCATTCTCAGTTCCAACATTGGCACCGGTGA

Coding sequence (CDS)

ATGGCTCTCTATGGAACCTTAATGCTTCTTCTTTCCTTTCTGTTACTTTTGCTTTTGAACCATGCCTTGGCTGATGGATTAACCTTAGATATCGTTAGTCTTGGAGCCAAGCCCGATGGAAAAATCGATGCCTCGCTTAATCTGGAAATCACAAATGCACAAAATGTTATCATCAATGGATTAGCTTCAATTAATAGCCAAATATTTAACATTATGGTGCACGGTTGCCAAAATGTGAAAATTCAAGGAGTGAAGGTCTCGGATCCTGACGATAGTTCGAACACTGATGGCTTCCATGTGCAGGAGTCCATCAGATGTGACCATTCTCAGTTCCAACATTGGCACCGGTGA

Protein sequence

MALYGTLMLLLSFLLLLLLNHALADGLTLDIVSLGAKPDGKIDASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQESIRCDHSQFQHWHR
Homology
[Close]
BLAST of CmaCh19G003170 vs. Swiss-Prot
Match: PGLR_PRUPE (Polygalacturonase OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 4.9e-09
Identity = 28/56 (50.00%), Postives = 45/56 (80.36%), Query Frame = 1 

Query: 48  LEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQES 104
           L  +++ N++++GLAS+NSQ+F+I+++  QNV++QGV+VS   +S NTDG HVQ S
Sbjct: 150 LGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVRVSRSGNSPNTDGIHVQMS 205

[Close]
BLAST of CmaCh19G003170 vs. Swiss-Prot
Match: PGLR6_ARATH (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.1e-07
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 1 

Query: 47  NLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQES 104
           +L  + + N+ I+GL SINSQ F+I++    NV I GVKVS  ++S NTDG HV+ S
Sbjct: 155 SLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVESS 211

[Close]
BLAST of CmaCh19G003170 vs. Swiss-Prot
Match: PGLR1_ARATH (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana GN=PGA3 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 6.7e-06
Identity = 27/73 (36.99%), Postives = 47/73 (64.38%), Query Frame = 1 

Query: 37  KPDG---KIDASL---NLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPD 96
           K DG   K+  +L   +L+  N +NV ING++S+N++ F++ +   +NV IQ +K++ P 
Sbjct: 155 KADGCGKKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPA 214

Query: 97  DSSNTDGFHVQES 104
           +S NTDG H+  +
Sbjct: 215 ESPNTDGIHLSNA 227

[Close]
BLAST of CmaCh19G003170 vs. TrEMBL
Match: A0A0A0KN91_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G409610 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-14
Identity = 42/67 (62.69%), Postives = 55/67 (82.09%), Query Frame = 1 

Query: 43  DASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQE 102
           D + NLEI++AQNV +NGL+SINSQ+FNI+V+GC+NV+IQGV VS   DS NTDG HVQ+
Sbjct: 143 DGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSSAGDSPNTDGIHVQQ 202

Query: 103 SIRCDHS 110
           S+  + S
Sbjct: 203 SLNVNIS 209

[Close]
BLAST of CmaCh19G003170 vs. TrEMBL
Match: A0A059AVP0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H00255 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 7.4e-12
Identity = 37/79 (46.84%), Postives = 56/79 (70.89%), Query Frame = 1 

Query: 25  DGLTLDIVSLGAKPDGKIDASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGV 84
           DG  +D+ +           + NL I+N++N++++GL+S+NSQ+F+I+V GCQNVK+QGV
Sbjct: 136 DGQGMDLWACKLSGKSCPSGATNLMISNSENILVSGLSSLNSQMFHIVVSGCQNVKMQGV 195

Query: 85  KVSDPDDSSNTDGFHVQES 104
           KVS+P  S NTDG H+  S
Sbjct: 196 KVSNPGTSPNTDGIHITAS 214

[Close]
BLAST of CmaCh19G003170 vs. TrEMBL
Match: A0A058ZUB7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L01371 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 9.7e-12
Identity = 42/83 (50.60%), Postives = 59/83 (71.08%), Query Frame = 1 

Query: 26  GLTLDIVSLG---AKPDGKI--DASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVK 85
           G TLD   +G    K  GK     + NL I+N++N++++GL+S+NSQ+F+I+V GCQNVK
Sbjct: 132 GGTLDGQGMGLWACKLSGKSCPSGATNLMISNSENILVSGLSSLNSQMFHIVVSGCQNVK 191

Query: 86  IQGVKVSDPDDSSNTDGFHVQES 104
           +QGVKVS+P  S NTDG H+  S
Sbjct: 192 MQGVKVSNPGTSPNTDGIHITAS 214

[Close]
BLAST of CmaCh19G003170 vs. TrEMBL
Match: B9N8Q6_POPTR (Polygalacturonase family protein OS=Populus trichocarpa GN=POPTR_0017s03740g PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.7e-11
Identity = 46/97 (47.42%), Postives = 62/97 (63.92%), Query Frame = 1 

Query: 12  SFLLLLLLNHALADGLTLDIVSLG---AKPDGK--IDASLNLEITNAQNVIINGLASINS 71
           ++L+   +N     G TLD    G    K  GK     + +LE +N+ N+ I GLAS+NS
Sbjct: 112 NWLMFEHVNGVTVSGGTLDGQGAGLWSCKNSGKGCPRGATSLEFSNSNNIAITGLASLNS 171

Query: 72  QIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQES 104
           Q+F+I++HGCQNVK+QGVKVS   +S NTDG HVQ S
Sbjct: 172 QLFHIVIHGCQNVKVQGVKVSAAGNSPNTDGIHVQSS 208

[Close]
BLAST of CmaCh19G003170 vs. TrEMBL
Match: U5FH48_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s03770g PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.7e-11
Identity = 35/57 (61.40%), Postives = 47/57 (82.46%), Query Frame = 1 

Query: 47  NLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQES 104
           +LE +N+ N+ I GLAS+NSQ+F+I+++GCQNVK+QGVKVS   +S NTDG HVQ S
Sbjct: 171 SLEFSNSNNIAITGLASLNSQLFHIVINGCQNVKVQGVKVSAAGNSPNTDGIHVQSS 227

[Close]
BLAST of CmaCh19G003170 vs. TAIR10
Match: AT3G59850.1 (AT3G59850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 66.2 bits (160), Expect = 1.5e-11
Identity = 29/52 (55.77%), Postives = 42/52 (80.77%), Query Frame = 1 

Query: 52  NAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQES 104
           +++NV+I+GL S+NSQ+F++ ++GC NVK+ GVKVS   +S NTDG HVQ S
Sbjct: 149 SSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVKVSADGNSPNTDGIHVQSS 200

[Close]
BLAST of CmaCh19G003170 vs. TAIR10
Match: AT2G43870.1 (AT2G43870.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 65.9 bits (159), Expect = 1.9e-11
Identity = 28/52 (53.85%), Postives = 42/52 (80.77%), Query Frame = 1 

Query: 52  NAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQES 104
           ++ NV+++GL S+NSQ+F+++++GC NVK+QGVKV    +S NTDG HVQ S
Sbjct: 147 SSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVLAAGNSPNTDGIHVQSS 198

[Close]
BLAST of CmaCh19G003170 vs. TAIR10
Match: AT1G65570.1 (AT1G65570.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 6.2e-10
Identity = 26/61 (42.62%), Postives = 43/61 (70.49%), Query Frame = 1 

Query: 43  DASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQE 102
           + +  L   ++ NV I G+ S+NSQ+F+I ++ C+N+KI+ V++  PD+S NTDG H+Q 
Sbjct: 149 EGATTLRFMDSNNVKIKGVLSLNSQLFHIAINRCRNIKIEDVRIIAPDESPNTDGIHIQL 208

Query: 103 S 104
           S
Sbjct: 209 S 209

[Close]
BLAST of CmaCh19G003170 vs. TAIR10
Match: AT1G05660.1 (AT1G05660.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 58.5 bits (140), Expect = 3.1e-09
Identity = 26/57 (45.61%), Postives = 43/57 (75.44%), Query Frame = 1 

Query: 47  NLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQES 104
           ++   +A++VII+G+ S+NSQ+ ++ ++GC NV ++ VK+  P +S NTDGFHVQ S
Sbjct: 147 SISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKLVAPGNSPNTDGFHVQHS 203

[Close]
BLAST of CmaCh19G003170 vs. TAIR10
Match: AT2G43860.1 (AT2G43860.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 56.6 bits (135), Expect = 1.2e-08
Identity = 28/57 (49.12%), Postives = 39/57 (68.42%), Query Frame = 1 

Query: 47  NLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQES 104
           +L  + + N+ I+GL SINSQ F+I++    NV I GVKVS  ++S NTDG HV+ S
Sbjct: 155 SLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVESS 211

[Close]
BLAST of CmaCh19G003170 vs. NCBI nr
Match: gi|449445220|ref|XP_004140371.1| (PREDICTED: polygalacturonase [Cucumis sativus])

HSP 1 Score: 87.4 bits (215), Expect = 1.8e-14
Identity = 42/67 (62.69%), Postives = 55/67 (82.09%), Query Frame = 1 

Query: 43  DASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQE 102
           D + NLEI++AQNV +NGL+SINSQ+FNI+V+GC+NV+IQGV VS   DS NTDG HVQ+
Sbjct: 143 DGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSSAGDSPNTDGIHVQQ 202

Query: 103 SIRCDHS 110
           S+  + S
Sbjct: 203 SLNVNIS 209

[Close]
BLAST of CmaCh19G003170 vs. NCBI nr
Match: gi|659121214|ref|XP_008460550.1| (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-13
Identity = 40/67 (59.70%), Postives = 54/67 (80.60%), Query Frame = 1 

Query: 43  DASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGVKVSDPDDSSNTDGFHVQE 102
           D + +LEI ++QNV +NGL+S+NSQ+FNI+V+GCQNV+IQGV VS   DS NTDG HVQ+
Sbjct: 143 DGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNVQIQGVNVSSASDSPNTDGIHVQQ 202

Query: 103 SIRCDHS 110
           S+  + S
Sbjct: 203 SLNVNIS 209

[Close]
BLAST of CmaCh19G003170 vs. NCBI nr
Match: gi|629091490|gb|KCW57485.1| (hypothetical protein EUGRSUZ_H00255 [Eucalyptus grandis])

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-11
Identity = 37/79 (46.84%), Postives = 56/79 (70.89%), Query Frame = 1 

Query: 25  DGLTLDIVSLGAKPDGKIDASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGV 84
           DG  +D+ +           + NL I+N++N++++GL+S+NSQ+F+I+V GCQNVK+QGV
Sbjct: 136 DGQGMDLWACKLSGKSCPSGATNLMISNSENILVSGLSSLNSQMFHIVVSGCQNVKMQGV 195

Query: 85  KVSDPDDSSNTDGFHVQES 104
           KVS+P  S NTDG H+  S
Sbjct: 196 KVSNPGTSPNTDGIHITAS 214

[Close]
BLAST of CmaCh19G003170 vs. NCBI nr
Match: gi|702432501|ref|XP_010069209.1| (PREDICTED: polygalacturonase-like [Eucalyptus grandis])

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-11
Identity = 37/79 (46.84%), Postives = 56/79 (70.89%), Query Frame = 1 

Query: 25  DGLTLDIVSLGAKPDGKIDASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVKIQGV 84
           DG  +D+ +           + NL I+N++N++++GL+S+NSQ+F+I+V GCQNVK+QGV
Sbjct: 135 DGQGMDLWACKLSGKSCPSGATNLMISNSENILVSGLSSLNSQMFHIVVSGCQNVKMQGV 194

Query: 85  KVSDPDDSSNTDGFHVQES 104
           KVS+P  S NTDG H+  S
Sbjct: 195 KVSNPGTSPNTDGIHITAS 213

[Close]
BLAST of CmaCh19G003170 vs. NCBI nr
Match: gi|629077463|gb|KCW45034.1| (hypothetical protein EUGRSUZ_L01371 [Eucalyptus grandis])

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-11
Identity = 42/83 (50.60%), Postives = 59/83 (71.08%), Query Frame = 1 

Query: 26  GLTLDIVSLG---AKPDGKI--DASLNLEITNAQNVIINGLASINSQIFNIMVHGCQNVK 85
           G TLD   +G    K  GK     + NL I+N++N++++GL+S+NSQ+F+I+V GCQNVK
Sbjct: 132 GGTLDGQGMGLWACKLSGKSCPSGATNLMISNSENILVSGLSSLNSQMFHIVVSGCQNVK 191

Query: 86  IQGVKVSDPDDSSNTDGFHVQES 104
           +QGVKVS+P  S NTDG H+  S
Sbjct: 192 MQGVKVSNPGTSPNTDGIHITAS 214

The following BLAST results are available for this feature:
BLAST of CmaCh19G003170 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PGLR_PRUPE4.9e-0950.00Polygalacturonase OS=Prunus persica PE=2 SV=1[more]
PGLR6_ARATH2.1e-0749.12Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 S... [more]
PGLR1_ARATH6.7e-0636.99Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana GN=PGA3 PE=2 SV=1[more]
BLAST of CmaCh19G003170 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KN91_CUCSA1.2e-1462.69Uncharacterized protein OS=Cucumis sativus GN=Csa_5G409610 PE=3 SV=1[more]
A0A059AVP0_EUCGR7.4e-1246.84Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H00255 PE=3 SV=1[more]
A0A058ZUB7_EUCGR9.7e-1250.60Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_L01371 PE=3 SV=1[more]
B9N8Q6_POPTR1.7e-1147.42Polygalacturonase family protein OS=Populus trichocarpa GN=POPTR_0017s03740g PE=... [more]
U5FH48_POPTR1.7e-1161.40Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s03770g PE=3 SV=1[more]
BLAST of CmaCh19G003170 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G59850.11.5e-1155.77 Pectin lyase-like superfamily protein[more]
AT2G43870.11.9e-1153.85 Pectin lyase-like superfamily protein[more]
AT1G65570.16.2e-1042.62 Pectin lyase-like superfamily protein[more]
AT1G05660.13.1e-0945.61 Pectin lyase-like superfamily protein[more]
AT2G43860.11.2e-0849.12 Pectin lyase-like superfamily protein[more]
BLAST of CmaCh19G003170 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449445220|ref|XP_004140371.1|1.8e-1462.69PREDICTED: polygalacturonase [Cucumis sativus][more]
gi|659121214|ref|XP_008460550.1|1.5e-1359.70PREDICTED: polygalacturonase-like [Cucumis melo][more]
gi|629091490|gb|KCW57485.1|1.1e-1146.84hypothetical protein EUGRSUZ_H00255 [Eucalyptus grandis][more]
gi|702432501|ref|XP_010069209.1|1.1e-1146.84PREDICTED: polygalacturonase-like [Eucalyptus grandis][more]
gi|629077463|gb|KCW45034.1|1.4e-1150.60hypothetical protein EUGRSUZ_L01371 [Eucalyptus grandis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0004650 polygalacturonase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh19G003170.1CmaCh19G003170.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 46..103
score: 7.2
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 14..105
score: 1.09
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 47..103
score: 1.6
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 48..103
score: 3.7
NoneNo IPR availablePANTHERPTHR31375:SF2POLYGALACTURONASE/PECTINASEcoord: 48..103
score: 3.7

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh19G003170CmoCh19G003870Cucurbita moschata (Rifu)cmacmoB511
CmaCh19G003170Lsi03G009010Bottle gourd (USVL1VR-Ls)cmalsiB482
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh19G003170Cucurbita moschata (Rifu)cmacmoB508
CmaCh19G003170Cucurbita moschata (Rifu)cmacmoB509
CmaCh19G003170Cucurbita pepo (Zucchini)cmacpeB522
CmaCh19G003170Watermelon (97103) v2cmawmbB551
CmaCh19G003170Wax gourdcmawgoB0655