BLAST of CmaCh18G012100 vs. Swiss-Prot
Match:
GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)
HSP 1 Score: 390.6 bits (1002), Expect = 2.2e-107
Identity = 186/337 (55.19%), Postives = 235/337 (69.73%), Query Frame = 1
Query: 33 ESKVPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFV 92
++ +P FIW + + S + LDVP IDL+ +S D S +A+RL+ EAC++HG F V
Sbjct: 37 QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96
Query: 93 VNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASNLPWKETFS 152
VNHG+ E++ H+ + FF MPL KQR RK GES GY +SF GRF++ LPWKET S
Sbjct: 97 VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156
Query: 153 LR-CVAAQNSSAAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVELLGLSLGISREY 212
R C S + DY D LG F GK YQE A++ L KI+ELLGLSLG+ R+Y
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216
Query: 213 FKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSI 272
F+ F+++NDSI+RLNYYP C KP++ LGTGPH DPTS+TILHQD V+GLQV +QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276
Query: 273 PPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSHDKVVRAPEELV 332
PNP+AFV+NIGDTF +L+N YK C+HRAVVNS + RKSLAFFLCP D+VV P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336
Query: 333 EKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
+ R+YPD+ W M LE TQK YR D NTL+AF W
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDW 372
BLAST of CmaCh18G012100 vs. Swiss-Prot
Match:
GAOX3_ARATH (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1)
HSP 1 Score: 386.7 bits (992), Expect = 3.2e-106
Identity = 185/337 (54.90%), Postives = 230/337 (68.25%), Query Frame = 1
Query: 36 VPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNH 95
+P+ F+W + + S + L VP IDL F+SGD EATRLV +A +HG F + NH
Sbjct: 37 IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96
Query: 96 GVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRC 155
GVD ++ R + M+ FF P KQ+A+RK GES GY +SF GRF+S LPWKET S +
Sbjct: 97 GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156
Query: 156 VAAQ--NSSAAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVELLGLSLGISREYFK 215
+ +S D+V +G + GK YQE A+N L KI+ELLG+SLG+ R YFK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216
Query: 216 NFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPP 275
F++D+DSI RLNYYP C +PE+ LGTGPH DPTS+TILHQD V GLQV +++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276
Query: 276 NPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSHDKVVRAPEELVE- 335
NP AFV+NIGDTF +LTNG YK C+HRAVVNS RK+ AFFLCP +KVV+ PEELV
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336
Query: 336 -KSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
KS RKYPD+ W M LE TQK YR D NTL+ F W
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIW 373
BLAST of CmaCh18G012100 vs. Swiss-Prot
Match:
GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1)
HSP 1 Score: 385.6 bits (989), Expect = 7.2e-106
Identity = 184/342 (53.80%), Postives = 241/342 (70.47%), Query Frame = 1
Query: 33 ESKVPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFV 92
+S++P FIW ++ + S +P L+VP IDL S D + EA R++ EAC +HG F V
Sbjct: 39 QSQIPNQFIWPDEEKPSIDIPELNVPFIDLS---SQDSTL--EAPRVIAEACTKHGFFLV 98
Query: 93 VNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASNLPWKETFS 152
VNHGV ++ H M FF MPL KQ+A+RK GES GY +SF GRF++ LPWKET S
Sbjct: 99 VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158
Query: 153 LRCVAAQNSS-AAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVELLGLSLGISREY 212
+ + S DY DTLG F GK YQ+ A++ L KI+ELLGLSLG++R+Y
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218
Query: 213 FKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSI 272
F+ F+++NDSI+RLN+YP C P++ LGTGPH DP+S+TILHQD V+GLQV ++QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278
Query: 273 PPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSHDKVVRAPEELV 332
PNP+AFV+NIGDTF +L+NGI+K C+HRAVVN +ARKS+AFFLCP DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338
Query: 333 EKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTWKLLRN 374
EK RKYPD+ W M LE TQK YR D NTL++F W + N
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVITNN 375
BLAST of CmaCh18G012100 vs. Swiss-Prot
Match:
GAOX4_ARATH (Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana GN=GA20OX4 PE=2 SV=1)
HSP 1 Score: 374.0 bits (959), Expect = 2.2e-102
Identity = 178/337 (52.82%), Postives = 224/337 (66.47%), Query Frame = 1
Query: 36 VPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNH 95
+P++F+W + + S+ +P+L VP IDL F+S D V EA RLV EA ++HG F V NH
Sbjct: 36 IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95
Query: 96 GVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRC 155
GVD ++ H M+ FF P K +A+RKVGE+ GY +SF GRF NLPWKET S
Sbjct: 96 GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155
Query: 156 VAAQNSS----AAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVELLGLSLGISREY 215
+ S +Y+ T+G + G YQE ++ L KI+ELLG+SLGI RE+
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215
Query: 216 FKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSI 275
F+ F++DN+SI RLNYYP C +P++VLGTGPH DPTS+TIL QD VSGLQV ++QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275
Query: 276 PPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSHDKVVRAPEELV 335
PP P+A V+NIGDT +LTNGIYK C+HRAVVN RK+LAFFLCP DKVV+ P EL
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335
Query: 336 EKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
R YPD+ W M LE T K YR D NTLE F W
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNW 369
BLAST of CmaCh18G012100 vs. Swiss-Prot
Match:
GAO1A_WHEAT (Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1)
HSP 1 Score: 364.4 bits (934), Expect = 1.7e-99
Identity = 181/348 (52.01%), Postives = 226/348 (64.94%), Query Frame = 1
Query: 25 RPPFGG---SDESKVPEDFIWSE-KFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLV 84
RP F S S +P FIW E + + L VP I++ +SGD + E TRLV
Sbjct: 3 RPVFDAAVLSGRSDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLV 62
Query: 85 DEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGR 144
EAC +HG F VVNHG+D E++ H C++ FFTMPL KQRA R+ GES GY +SF GR
Sbjct: 63 GEACERHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGR 122
Query: 145 FASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVEL 204
FAS LPWKET S R + + DY++ TLG G+ Y ++ L +I+E+
Sbjct: 123 FASKLPWKETLSFRSCPSDPALVV-DYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEV 182
Query: 205 LGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGL 264
LG SLG+ R +++ F++ NDSI+RLNYYP C +P LGTGPH DPTS+TILHQD V GL
Sbjct: 183 LGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGL 242
Query: 265 QVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSH 324
QV + +W SI P +AFV+NIGDTF +L+NG YK C+HRAVVNS RKSLAFFLCP
Sbjct: 243 QVHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEM 302
Query: 325 DKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
DKVV P LV+ S PR YPD+ W LL+ TQK YR D TLE F +W
Sbjct: 303 DKVVAPPGTLVDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSW 349
BLAST of CmaCh18G012100 vs. TrEMBL
Match:
O23762_CUCMA (Gibberellin 20-oxidase OS=Cucurbita maxima PE=2 SV=1)
HSP 1 Score: 766.1 bits (1977), Expect = 2.2e-218
Identity = 367/368 (99.73%), Postives = 367/368 (99.73%), Query Frame = 1
Query: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI
Sbjct: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
Query: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK
Sbjct: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
Query: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG
Sbjct: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
Query: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240
KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT
Sbjct: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240
Query: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR
Sbjct: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
Query: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360
AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRP CN
Sbjct: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPACN 360
Query: 361 TLEAFKTW 369
TLEAFKTW
Sbjct: 361 TLEAFKTW 368
BLAST of CmaCh18G012100 vs. TrEMBL
Match:
Q39541_CUCMA (Gibberellin 20-oxidase OS=Cucurbita maxima PE=2 SV=1)
HSP 1 Score: 764.2 bits (1972), Expect = 8.3e-218
Identity = 365/368 (99.18%), Postives = 367/368 (99.73%), Query Frame = 1
Query: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI
Sbjct: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
Query: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK
Sbjct: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
Query: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLG SFSHHG
Sbjct: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGPSFSHHG 180
Query: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240
KAYQECGIALNELGTKIVELLGLSLGISREYFKNF++DNDSILRLNYYPTCDKPEVVLGT
Sbjct: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFFEDNDSILRLNYYPTCDKPEVVLGT 240
Query: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR
Sbjct: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
Query: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360
AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN
Sbjct: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360
Query: 361 TLEAFKTW 369
TLEAFKTW
Sbjct: 361 TLEAFKTW 368
BLAST of CmaCh18G012100 vs. TrEMBL
Match:
Q96498_9ROSI (Gibberellin 20-oxidase OS=Marah macrocarpus PE=2 SV=1)
HSP 1 Score: 536.6 bits (1381), Expect = 2.8e-149
Identity = 252/374 (67.38%), Postives = 304/374 (81.28%), Query Frame = 1
Query: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
MA+ +ATES+P+ EE+K E++ FGG+ ES VPE+FIW ++F+A+ P L VP I
Sbjct: 1 MAITCMMATESSPA---EEVKREHQVAFGGAGESSVPENFIWPDEFKATADAPELHVPHI 60
Query: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
DL+K +SGD+ VEEATRLVDEACR+HG F VVNHGVD E+M +VH+CMNEFFT+PLDVK
Sbjct: 61 DLKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDVK 120
Query: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
Q+A RKVGE++GY NSF GRF++ LPWKETFSLR +A +NSS A DYV LG FSHHG
Sbjct: 121 QKAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAHENSSTARDYVSQVLGPEFSHHG 180
Query: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240
+ YQECG AL++L +IVELLGLSLGISRE F+ FY+DNDSI+R+NYYP C+KPE+ LGT
Sbjct: 181 EVYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTLGT 240
Query: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
GPH DPTS+TILHQD VSGLQV +DQW+SIPP ++FV+N+GDTF SLTNG+YK C HR
Sbjct: 241 GPHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCFHR 300
Query: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360
AVVN ARKS+AFFLCP+ +KVVRAPEELVEK PPRK+PDY WPMLLEMTQK YR D N
Sbjct: 301 AVVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYYRADSN 360
Query: 361 TLEAFKTWKLLRNI 375
T AF TW +N+
Sbjct: 361 TFNAFTTWIQQQNL 371
BLAST of CmaCh18G012100 vs. TrEMBL
Match:
I0J1I7_CUCMA (Gibberellin 20-oxidase OS=Cucurbita maxima GN=ga20ox4 PE=2 SV=1)
HSP 1 Score: 519.6 bits (1337), Expect = 3.6e-144
Identity = 240/367 (65.40%), Postives = 294/367 (80.11%), Query Frame = 1
Query: 8 ATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLEKFMS 67
A S L +EMK ++R FG SDES VPE+FIW ++F+A E +P LDVP IDL+KF+S
Sbjct: 9 AKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPELDVPHIDLQKFVS 68
Query: 68 GDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKV 127
+++ +EEATRLVDEACR+HG F +VNHGVD+E+M VH+CM+EFF++PLDVKQ+A+RKV
Sbjct: 69 ENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDVKQKAQRKV 128
Query: 128 GESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGKAYQECG 187
GE+YGY NSF GRF+S LPWKETFSLR A N HDYV LG FS GK Y+ECG
Sbjct: 129 GENYGYANSFIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQGKVYEECG 188
Query: 188 IALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPT 247
AL++L KIVELLGLSLG+ +E FK F++DNDSI+RLNYYP C+KPE+ LGTGPH DPT
Sbjct: 189 EALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLGTGPHCDPT 248
Query: 248 SVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMN 307
S+TILHQD VSGLQV +DQW+SIPP ++FVINIGDTF +LTNG+YK C HRAVVN
Sbjct: 249 SITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFHRAVVNCRE 308
Query: 308 ARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKT 367
ARKS+ FFLCP+ DKVVRAP+E+V+K+PPRK+PDY WPMLLE+TQK YR D NT +AF T
Sbjct: 309 ARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDSNTFKAFTT 368
Query: 368 WKLLRNI 375
W +N+
Sbjct: 369 WLQHKNL 375
BLAST of CmaCh18G012100 vs. TrEMBL
Match:
Q9XHR0_CITLA (Gibberellin 20-oxidase OS=Citrullus lanatus GN=WGA20OXI PE=2 SV=1)
HSP 1 Score: 496.9 bits (1278), Expect = 2.5e-137
Identity = 228/353 (64.59%), Postives = 286/353 (81.02%), Query Frame = 1
Query: 17 NEEMKGE-YRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEE 76
++EMK + +R FG S+ES VPE+F+W ++F+A + P L VP IDL KF++G++S +EE
Sbjct: 12 SKEMKSKLHRVEFGCSEESMVPENFVWPDEFKAKDGAPELHVPHIDLHKFLNGNESDIEE 71
Query: 77 ATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTN 136
ATRLVDEACR+HG F +VNHGVD+E++ VH+CM+EFFT+P DVKQ+++RK GE+YGY N
Sbjct: 72 ATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQKSQRKFGENYGYAN 131
Query: 137 SFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGKAYQECGIALNELGT 196
SF GRF++NLPWKET SL VA NS++ D+V +G SH GK YQECG AL++L
Sbjct: 132 SFIGRFSNNLPWKETLSLPYVANHNSTSVQDFVSKCVGPELSHQGKVYQECGKALSDLSL 191
Query: 197 KIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQD 256
KIVELLGLSLG+ +E FK FY+DNDSI+RLNYYP C+KPE+ LGTGPH DPTS+TILHQD
Sbjct: 192 KIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQD 251
Query: 257 PVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFF 316
VSGLQV +D+W+SIPP ++FVINIGDTF +LTNG+YK C HRAVVN RKS+AFF
Sbjct: 252 HVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEVRKSMAFF 311
Query: 317 LCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
LCP+ DKVVRAP+E+VEK+PPRK+PDY WPMLLE+TQK YR D NT +AF W
Sbjct: 312 LCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFQAFIPW 364
BLAST of CmaCh18G012100 vs. TAIR10
Match:
AT4G25420.1 (AT4G25420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 390.6 bits (1002), Expect = 1.3e-108
Identity = 186/337 (55.19%), Postives = 235/337 (69.73%), Query Frame = 1
Query: 33 ESKVPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFV 92
++ +P FIW + + S + LDVP IDL+ +S D S +A+RL+ EAC++HG F V
Sbjct: 37 QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLV 96
Query: 93 VNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASNLPWKETFS 152
VNHG+ E++ H+ + FF MPL KQR RK GES GY +SF GRF++ LPWKET S
Sbjct: 97 VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156
Query: 153 LR-CVAAQNSSAAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVELLGLSLGISREY 212
R C S + DY D LG F GK YQE A++ L KI+ELLGLSLG+ R+Y
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216
Query: 213 FKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSI 272
F+ F+++NDSI+RLNYYP C KP++ LGTGPH DPTS+TILHQD V+GLQV +QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276
Query: 273 PPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSHDKVVRAPEELV 332
PNP+AFV+NIGDTF +L+N YK C+HRAVVNS + RKSLAFFLCP D+VV P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336
Query: 333 EKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
+ R+YPD+ W M LE TQK YR D NTL+AF W
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDW 372
BLAST of CmaCh18G012100 vs. TAIR10
Match:
AT5G07200.1 (AT5G07200.1 gibberellin 20-oxidase 3)
HSP 1 Score: 386.7 bits (992), Expect = 1.8e-107
Identity = 185/337 (54.90%), Postives = 230/337 (68.25%), Query Frame = 1
Query: 36 VPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNH 95
+P+ F+W + + S + L VP IDL F+SGD EATRLV +A +HG F + NH
Sbjct: 37 IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96
Query: 96 GVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRC 155
GVD ++ R + M+ FF P KQ+A+RK GES GY +SF GRF+S LPWKET S +
Sbjct: 97 GVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKF 156
Query: 156 VAAQ--NSSAAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVELLGLSLGISREYFK 215
+ +S D+V +G + GK YQE A+N L KI+ELLG+SLG+ R YFK
Sbjct: 157 SPEEKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFK 216
Query: 216 NFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPP 275
F++D+DSI RLNYYP C +PE+ LGTGPH DPTS+TILHQD V GLQV +++W SIPP
Sbjct: 217 EFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPP 276
Query: 276 NPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSHDKVVRAPEELVE- 335
NP AFV+NIGDTF +LTNG YK C+HRAVVNS RK+ AFFLCP +KVV+ PEELV
Sbjct: 277 NPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNG 336
Query: 336 -KSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
KS RKYPD+ W M LE TQK YR D NTL+ F W
Sbjct: 337 VKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIW 373
BLAST of CmaCh18G012100 vs. TAIR10
Match:
AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)
HSP 1 Score: 385.6 bits (989), Expect = 4.1e-107
Identity = 184/342 (53.80%), Postives = 241/342 (70.47%), Query Frame = 1
Query: 33 ESKVPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFV 92
+S++P FIW ++ + S +P L+VP IDL S D + EA R++ EAC +HG F V
Sbjct: 39 QSQIPNQFIWPDEEKPSIDIPELNVPFIDLS---SQDSTL--EAPRVIAEACTKHGFFLV 98
Query: 93 VNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASNLPWKETFS 152
VNHGV ++ H M FF MPL KQ+A+RK GES GY +SF GRF++ LPWKET S
Sbjct: 99 VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158
Query: 153 LRCVAAQNSS-AAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVELLGLSLGISREY 212
+ + S DY DTLG F GK YQ+ A++ L KI+ELLGLSLG++R+Y
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218
Query: 213 FKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSI 272
F+ F+++NDSI+RLN+YP C P++ LGTGPH DP+S+TILHQD V+GLQV ++QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278
Query: 273 PPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSHDKVVRAPEELV 332
PNP+AFV+NIGDTF +L+NGI+K C+HRAVVN +ARKS+AFFLCP DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338
Query: 333 EKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTWKLLRN 374
EK RKYPD+ W M LE TQK YR D NTL++F W + N
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVITNN 375
BLAST of CmaCh18G012100 vs. TAIR10
Match:
AT1G60980.1 (AT1G60980.1 gibberellin 20-oxidase 4)
HSP 1 Score: 374.0 bits (959), Expect = 1.2e-103
Identity = 178/337 (52.82%), Postives = 224/337 (66.47%), Query Frame = 1
Query: 36 VPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNH 95
+P++F+W + + S+ +P+L VP IDL F+S D V EA RLV EA ++HG F V NH
Sbjct: 36 IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95
Query: 96 GVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRC 155
GVD ++ H M+ FF P K +A+RKVGE+ GY +SF GRF NLPWKET S
Sbjct: 96 GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155
Query: 156 VAAQNSS----AAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVELLGLSLGISREY 215
+ S +Y+ T+G + G YQE ++ L KI+ELLG+SLGI RE+
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215
Query: 216 FKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSI 275
F+ F++DN+SI RLNYYP C +P++VLGTGPH DPTS+TIL QD VSGLQV ++QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275
Query: 276 PPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSHDKVVRAPEELV 335
PP P+A V+NIGDT +LTNGIYK C+HRAVVN RK+LAFFLCP DKVV+ P EL
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335
Query: 336 EKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
R YPD+ W M LE T K YR D NTLE F W
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNW 369
BLAST of CmaCh18G012100 vs. TAIR10
Match:
AT1G44090.1 (AT1G44090.1 gibberellin 20-oxidase 5)
HSP 1 Score: 281.2 bits (718), Expect = 1.1e-75
Identity = 142/338 (42.01%), Postives = 197/338 (58.28%), Query Frame = 1
Query: 33 ESKVPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEEATRLVDEACRQHGIFFV 92
+ VP +F W EK + + LD+P IDL F++G+++ + A + V +AC HG F V
Sbjct: 40 QPNVPAEFFWPEK-DVAPSEGDLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLV 99
Query: 93 VNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTNSFFGRFASNLPWKETFS 152
VNHG + + + + FF + D K RA R G GYT RF+SNLPW ET +
Sbjct: 100 VNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLT 159
Query: 153 LRCVAAQNSSAAHDYVLDTLGSSFSHHGKAYQECGIALNELGTKIVELLGLSLGIS-REY 212
L D++ LG+ G+ +QE A+N L ++ELLG+S+G+ R Y
Sbjct: 160 LAFKKGP-PHVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDRTY 219
Query: 213 FKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQDPVSGLQVCSNDQWYSI 272
++ F++D I R NYYP C +PE LG GPH DPT++T+L QD V GL+V + W ++
Sbjct: 220 YRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQTV 279
Query: 273 PPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFFLCPSHDKVVRAPEELV 332
P P A V+N+GDTF +L+NG Y+ C HRAVVN R+SL FF CP DK++ P ELV
Sbjct: 280 RPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPELV 339
Query: 333 E-KSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
E + RKYPD+ W L + TQ YR D TL F +W
Sbjct: 340 EGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSW 375
BLAST of CmaCh18G012100 vs. NCBI nr
Match:
gi|2224890|gb|AAB64345.1| (gibberellin 20-oxidase [Cucurbita maxima])
HSP 1 Score: 766.1 bits (1977), Expect = 3.1e-218
Identity = 367/368 (99.73%), Postives = 367/368 (99.73%), Query Frame = 1
Query: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI
Sbjct: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
Query: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK
Sbjct: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
Query: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG
Sbjct: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
Query: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240
KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT
Sbjct: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240
Query: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR
Sbjct: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
Query: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360
AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRP CN
Sbjct: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPACN 360
Query: 361 TLEAFKTW 369
TLEAFKTW
Sbjct: 361 TLEAFKTW 368
BLAST of CmaCh18G012100 vs. NCBI nr
Match:
gi|486625|emb|CAA51744.1| (gibberellin 20-oxidase [Cucurbita maxima])
HSP 1 Score: 764.2 bits (1972), Expect = 1.2e-217
Identity = 365/368 (99.18%), Postives = 367/368 (99.73%), Query Frame = 1
Query: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI
Sbjct: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
Query: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK
Sbjct: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
Query: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLG SFSHHG
Sbjct: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGPSFSHHG 180
Query: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240
KAYQECGIALNELGTKIVELLGLSLGISREYFKNF++DNDSILRLNYYPTCDKPEVVLGT
Sbjct: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFFEDNDSILRLNYYPTCDKPEVVLGT 240
Query: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR
Sbjct: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
Query: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360
AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN
Sbjct: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360
Query: 361 TLEAFKTW 369
TLEAFKTW
Sbjct: 361 TLEAFKTW 368
BLAST of CmaCh18G012100 vs. NCBI nr
Match:
gi|1666094|emb|CAA70329.1| (gibberellin 20-oxidase [Marah macrocarpus])
HSP 1 Score: 536.6 bits (1381), Expect = 4.0e-149
Identity = 252/374 (67.38%), Postives = 304/374 (81.28%), Query Frame = 1
Query: 1 MALNGKVATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTI 60
MA+ +ATES+P+ EE+K E++ FGG+ ES VPE+FIW ++F+A+ P L VP I
Sbjct: 1 MAITCMMATESSPA---EEVKREHQVAFGGAGESSVPENFIWPDEFKATADAPELHVPHI 60
Query: 61 DLEKFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVK 120
DL+K +SGD+ VEEATRLVDEACR+HG F VVNHGVD E+M +VH+CMNEFFT+PLDVK
Sbjct: 61 DLKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDVK 120
Query: 121 QRAKRKVGESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHG 180
Q+A RKVGE++GY NSF GRF++ LPWKETFSLR +A +NSS A DYV LG FSHHG
Sbjct: 121 QKAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAHENSSTARDYVSQVLGPEFSHHG 180
Query: 181 KAYQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGT 240
+ YQECG AL++L +IVELLGLSLGISRE F+ FY+DNDSI+R+NYYP C+KPE+ LGT
Sbjct: 181 EVYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTLGT 240
Query: 241 GPHTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHR 300
GPH DPTS+TILHQD VSGLQV +DQW+SIPP ++FV+N+GDTF SLTNG+YK C HR
Sbjct: 241 GPHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCFHR 300
Query: 301 AVVNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCN 360
AVVN ARKS+AFFLCP+ +KVVRAPEELVEK PPRK+PDY WPMLLEMTQK YR D N
Sbjct: 301 AVVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYYRADSN 360
Query: 361 TLEAFKTWKLLRNI 375
T AF TW +N+
Sbjct: 361 TFNAFTTWIQQQNL 371
BLAST of CmaCh18G012100 vs. NCBI nr
Match:
gi|384368293|emb|CBL59326.1| (Gibberellin 20-oxidase [Cucurbita maxima])
HSP 1 Score: 519.6 bits (1337), Expect = 5.1e-144
Identity = 240/367 (65.40%), Postives = 294/367 (80.11%), Query Frame = 1
Query: 8 ATESAPSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLEKFMS 67
A S L +EMK ++R FG SDES VPE+FIW ++F+A E +P LDVP IDL+KF+S
Sbjct: 9 AKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPELDVPHIDLQKFVS 68
Query: 68 GDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKV 127
+++ +EEATRLVDEACR+HG F +VNHGVD+E+M VH+CM+EFF++PLDVKQ+A+RKV
Sbjct: 69 ENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDVKQKAQRKV 128
Query: 128 GESYGYTNSFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGKAYQECG 187
GE+YGY NSF GRF+S LPWKETFSLR A N HDYV LG FS GK Y+ECG
Sbjct: 129 GENYGYANSFIGRFSSKLPWKETFSLRYAAHHNLPVTHDYVCHHLGPEFSQQGKVYEECG 188
Query: 188 IALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPT 247
AL++L KIVELLGLSLG+ +E FK F++DNDSI+RLNYYP C+KPE+ LGTGPH DPT
Sbjct: 189 EALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLGTGPHCDPT 248
Query: 248 SVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMN 307
S+TILHQD VSGLQV +DQW+SIPP ++FVINIGDTF +LTNG+YK C HRAVVN
Sbjct: 249 SITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFHRAVVNCRE 308
Query: 308 ARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKT 367
ARKS+ FFLCP+ DKVVRAP+E+V+K+PPRK+PDY WPMLLE+TQK YR D NT +AF T
Sbjct: 309 ARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDSNTFKAFTT 368
Query: 368 WKLLRNI 375
W +N+
Sbjct: 369 WLQHKNL 375
BLAST of CmaCh18G012100 vs. NCBI nr
Match:
gi|5359492|gb|AAD42693.1|AF074709_1 (gibberellin 20-oxidase [Citrullus lanatus subsp. vulgaris])
HSP 1 Score: 496.9 bits (1278), Expect = 3.5e-137
Identity = 228/353 (64.59%), Postives = 286/353 (81.02%), Query Frame = 1
Query: 17 NEEMKGE-YRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLEKFMSGDKSYVEE 76
++EMK + +R FG S+ES VPE+F+W ++F+A + P L VP IDL KF++G++S +EE
Sbjct: 12 SKEMKSKLHRVEFGCSEESMVPENFVWPDEFKAKDGAPELHVPHIDLHKFLNGNESDIEE 71
Query: 77 ATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRAKRKVGESYGYTN 136
ATRLVDEACR+HG F +VNHGVD+E++ VH+CM+EFFT+P DVKQ+++RK GE+YGY N
Sbjct: 72 ATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQKSQRKFGENYGYAN 131
Query: 137 SFFGRFASNLPWKETFSLRCVAAQNSSAAHDYVLDTLGSSFSHHGKAYQECGIALNELGT 196
SF GRF++NLPWKET SL VA NS++ D+V +G SH GK YQECG AL++L
Sbjct: 132 SFIGRFSNNLPWKETLSLPYVANHNSTSVQDFVSKCVGPELSHQGKVYQECGKALSDLSL 191
Query: 197 KIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGTGPHTDPTSVTILHQD 256
KIVELLGLSLG+ +E FK FY+DNDSI+RLNYYP C+KPE+ LGTGPH DPTS+TILHQD
Sbjct: 192 KIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGPHCDPTSITILHQD 251
Query: 257 PVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAVVNSMNARKSLAFF 316
VSGLQV +D+W+SIPP ++FVINIGDTF +LTNG+YK C HRAVVN RKS+AFF
Sbjct: 252 HVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAVVNCKEVRKSMAFF 311
Query: 317 LCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTLEAFKTW 369
LCP+ DKVVRAP+E+VEK+PPRK+PDY WPMLLE+TQK YR D NT +AF W
Sbjct: 312 LCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTFQAFIPW 364
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| GAOX1_ARATH | 2.2e-107 | 55.19 | Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2 | [more] |
| GAOX3_ARATH | 3.2e-106 | 54.90 | Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1 | [more] |
| GAOX2_ARATH | 7.2e-106 | 53.80 | Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1 | [more] |
| GAOX4_ARATH | 2.2e-102 | 52.82 | Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana GN=GA20OX4 PE=2 SV=1 | [more] |
| GAO1A_WHEAT | 1.7e-99 | 52.01 | Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| O23762_CUCMA | 2.2e-218 | 99.73 | Gibberellin 20-oxidase OS=Cucurbita maxima PE=2 SV=1 | [more] |
| Q39541_CUCMA | 8.3e-218 | 99.18 | Gibberellin 20-oxidase OS=Cucurbita maxima PE=2 SV=1 | [more] |
| Q96498_9ROSI | 2.8e-149 | 67.38 | Gibberellin 20-oxidase OS=Marah macrocarpus PE=2 SV=1 | [more] |
| I0J1I7_CUCMA | 3.6e-144 | 65.40 | Gibberellin 20-oxidase OS=Cucurbita maxima GN=ga20ox4 PE=2 SV=1 | [more] |
| Q9XHR0_CITLA | 2.5e-137 | 64.59 | Gibberellin 20-oxidase OS=Citrullus lanatus GN=WGA20OXI PE=2 SV=1 | [more] |
