CmaCh18G009140 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh18G009140
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionLimit dextrinase type starch debranching enzyme
LocationCma_Chr18 : 7833734 .. 7835700 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTACTACGCTCAAAATCAATGGATCGTGATTCATATAACTCCGGTGATTGGTTCAACAGGTCCTGTTCTTTTTTTTTCTAAATCAATGGATCGTGATTCATATAACTCCGGTGATTGGTTCAACAGGTCCTGTTCTTTTTTTTTCTTTTCTTTTTGCATTCAATTATTTATAACATTCGTAAGTATTTAGATGTTGAGGGCACCGACTACAGTCTTTTAAAAAAAAAATTGTTCTAGTTTTCTGCAAATTTTATGCAGTACATAATTTGGAACATATGGTATATTACACATTGGGTATCAAATCCCATTTTCTCTAACTTAATATGGTATACTGCCAGTTATGGTTTGTCTTTGTTTACCTTGAATTGATTTTTACTTTTCTCAAAATCAGACTCGACTTCACTTACATGACAAATAATTGGGGCGTTGGCCTTCCTCCAAAAGAGAAGAATGAATACAATTGGCCTCTGTAAGTTTTACGAAATTTGCTAGATTTTGAGTAGCGTTCCTTCTGCAAGTCTTCCTTCCCCTTCTGGAATCTGGAATAGATGGAACTTATTCCTAGTCTTAAAAAATTTGTGGGAGGTTTACTGGAAAGCTTTGTTAATAATGTCATCCATTTTCAGAATCAAACCAAGACTGGCCGATCCCTCCTTCAAACCTAGTAAAAGTCACATTCTTGCTGCTGTTCAGAATCAAACCAAGACTGGCCGATCCCTCCTTCAAACCTAGTAAAAGTCACATTCTTGCTGCTGTGGAAAATTTTACAAATCTGTTACAAATCAGATACTCTTCACCACTTTTTCGTTTGAAGACAGCTAATGCTATCCAGGTAAGAATGCATGTTTAAGAATAATTGTCAGATGAAATATAATGTAAATTTTTAATTCAGCTGGTTAATCTTGGGGACTTGAGATCCATATTCAATAAAACAGTCTTTTTCTTGTACAAGAGCAACATAATACAGGGCTCAAAGCATCATTACTTTGTTTCAGGCTGGTCCACAATTTACACTGCTTTATTTCAATCTCTACTTTTCGACATGCAAAATTGAAATGTTATGGTGGTCCACAATTTACACTGCTTTATTTCAATCTCTACTTTTCGACATGCAAAATTGAAATGTTATGGTTGAACCAGTTAAACTGGACATAACGTAGCACCAGCTATTCAAACAGATATCAGTTAGCCTGCTTGTTTAAAGGCTTAGATGTATGACATCATTAGCTCGGTTTCCCTAGAATTGCTNCACACACGTCTGGTTTGGATTGGAAAGGAATTATGACATCATTAGCTCGGTTTCCCTAGAATTGCTACACACACGTCTGGTTTGGATTGGAAAGGAATGTACTTTTCTGGTGCTTGTGATGTGAAGTGCCTTTGCCTTTTCTTGTGTAGAAACGGGTCCGTTTCCACAATACAGGCACGTCGCTTATACCTGGAGTCATAGTGATGAGCATTGAAGATGGGCAGGAGTTTCCACAATACAGGCACGTCGCTTATACCTGGAGTCATAGTGATGAGCATTGAAGATGGGCAGGAAGGCATTCCTGGGTTAACTCAGCTGGATCCCATGTGAGTAGAATAGCTTTCTCTAAATCCATGATTTTACTCAATAATATTGTTATCTCATGTAATCTGCAGTTACTCCTACATTGTTGTTGTGGTTAATGCACGCCCATCTGAGATTTCATTTCCTTGTCCTGTATTGCGCGCAAGAACTCTCCAATTACATCCCATTCAGGTAAATAGATACTCTCCTGAGATCCAACACTGCTTCGCTCCAAAAGAGAACTTTTGCTAACTATAGCTCTTATCTTTATTATTTGTGCTATGTCAACGTTTTAAATATGGCAGTTGATGTCAACCGATCCAATGGTTAAAAATTCAGCTTACGAGCCATCCACAGGATGCTTCATGGTGCCCTCAAGAACCACATCAGTATTTGTGGAGCTACGGTAG

mRNA sequence

ATGAATTACTACGCTCAAAATCAATGGATCGTGATTCATATAACTCCGGTGATTGGTTCAACAGGTCCTGTTCTTTTTTTTTCTAAATCAATGGATCGTGATTCATATAACTCCGGTGATTGGTTCAACAGACTCGACTTCACTTACATGACAAATAATTGGGGCGTTGGCCTTCCTCCAAAAGAGAAGAATGAATACAATTGGCCTCTAATCAAACCAAGACTGGCCGATCCCTCCTTCAAACCTAGTAAAAGTCACATTCTTGCTGCTGTGGAAAATTTTACAAATCTGTTACAAATCAGATACTCTTCACCACTTTTTCGTTTGAAGACAGCTAATGCTATCCAGAAACGGATGGGCAGGAGTTTCCACAATACAGGCACGTCGCTTATACCTGGAGTCATAGTGATGAGCATTGAAGATGGGCAGGAAGGCATTCCTGGGTTAACTCAGCTGGATCCCATTTACTCCTACATTGTTGTTGTGGTTAATGCACGCCCATCTGAGATTTCATTTCCTTGTCCTGTATTGCGCGCAAGAACTCTCCAATTACATCCCATTCAGTTGATGTCAACCGATCCAATGGTTAAAAATTCAGCTTACGAGCCATCCACAGGATGCTTCATGGTGCCCTCAAGAACCACATCAGTATTTGTGGAGCTACGGTAG

Coding sequence (CDS)

ATGAATTACTACGCTCAAAATCAATGGATCGTGATTCATATAACTCCGGTGATTGGTTCAACAGGTCCTGTTCTTTTTTTTTCTAAATCAATGGATCGTGATTCATATAACTCCGGTGATTGGTTCAACAGACTCGACTTCACTTACATGACAAATAATTGGGGCGTTGGCCTTCCTCCAAAAGAGAAGAATGAATACAATTGGCCTCTAATCAAACCAAGACTGGCCGATCCCTCCTTCAAACCTAGTAAAAGTCACATTCTTGCTGCTGTGGAAAATTTTACAAATCTGTTACAAATCAGATACTCTTCACCACTTTTTCGTTTGAAGACAGCTAATGCTATCCAGAAACGGATGGGCAGGAGTTTCCACAATACAGGCACGTCGCTTATACCTGGAGTCATAGTGATGAGCATTGAAGATGGGCAGGAAGGCATTCCTGGGTTAACTCAGCTGGATCCCATTTACTCCTACATTGTTGTTGTGGTTAATGCACGCCCATCTGAGATTTCATTTCCTTGTCCTGTATTGCGCGCAAGAACTCTCCAATTACATCCCATTCAGTTGATGTCAACCGATCCAATGGTTAAAAATTCAGCTTACGAGCCATCCACAGGATGCTTCATGGTGCCCTCAAGAACCACATCAGTATTTGTGGAGCTACGGTAG

Protein sequence

MNYYAQNQWIVIHITPVIGSTGPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFVELR
Homology
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BLAST of CmaCh18G009140 vs. Swiss-Prot
Match: PULA1_ARATH (Pullulanase 1, chloroplastic OS=Arabidopsis thaliana GN=PU1 PE=2 SV=2)

HSP 1 Score: 288.1 bits (736), Expect = 8.1e-77
Identity = 137/201 (68.16%), Postives = 162/201 (80.60%), Query Frame = 1 

Query: 22  GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
           G  +  SKS+DRDSYNSGDWFNRLDF+Y +NNWGVGLPPK KNE+NWPLIKPRL DPSFK
Sbjct: 765 GDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFK 824

Query: 82  PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
           P  SHI+A + NF +LL+IRYSSPLFRL TA AIQ+R+   FHNTG S IPG IVMSIED
Sbjct: 825 PKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERV--RFHNTGPSSIPGAIVMSIED 884

Query: 142 GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
           G  GIP ++Q+DPIYS IVV+ NARPSE S+P P L+ R L+LHP+Q+MS D +VK S Y
Sbjct: 885 GHRGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVY 944

Query: 202 EPSTGCFMVPSRTTSVFVELR 223
           +  +G F VP+RTT+VFVE R
Sbjct: 945 DSFSGGFTVPARTTTVFVESR 963

[Close]
BLAST of CmaCh18G009140 vs. Swiss-Prot
Match: PULA_KLEPN (Pullulanase OS=Klebsiella pneumoniae GN=pulA PE=1 SV=2)

HSP 1 Score: 101.3 bits (251), Expect = 1.4e-20
Identity = 64/196 (32.65%), Postives = 101/196 (51.53%), Query Frame = 1 

Query: 22   GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
            G  L  SKS  RDSY+SGDWFNR+D++   NN+ VG+P    +  N+ +I  R+ +    
Sbjct: 885  GSELLRSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRISDDGSNYEVI-TRVKEMVAT 944

Query: 82   PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
            P ++ +      +  L ++R SSPLF L   +A+ KR+   F NTG+    G++VM+++D
Sbjct: 945  PGEAELKQMTAFYQELTELRKSSPLFTLGDGSAVMKRV--DFRNTGSDQQAGLLVMTVDD 1004

Query: 142  GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
            G +       LD     +VV +NA P   S         TLQL  IQ  + +  + N   
Sbjct: 1005 GMK---AGASLDSRLDGLVVAINAAPE--SRTLNEFAGETLQLSAIQQTAGENSLANGVQ 1064

Query: 202  EPSTGCFMVPSRTTSV 218
              + G   +P+ + +V
Sbjct: 1065 IAADGTVTLPAWSVAV 1072

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BLAST of CmaCh18G009140 vs. Swiss-Prot
Match: PULA_ENTAE (Pullulanase OS=Enterobacter aerogenes GN=pulA PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.2e-17
Identity = 47/123 (38.21%), Postives = 69/123 (56.10%), Query Frame = 1 

Query: 22   GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
            G  L  SKS  RDSY+SGDWFNR+D++   NN+ VG+P    +  N+ +I  R+ D    
Sbjct: 895  GSELLRSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRSSDDGSNYDII-ARVKDAVAT 954

Query: 82   PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
            P ++ +      +  L  +R SSPLF L     + KR+   F NTG     G++VM+I+D
Sbjct: 955  PGETELKQMTAFYQELTALRKSSPLFTLGDGATVMKRV--DFRNTGADQQTGLLVMTIDD 1014

Query: 142  GQE 145
            G +
Sbjct: 1015 GMQ 1014

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BLAST of CmaCh18G009140 vs. TrEMBL
Match: A0A0A0KTI2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G622510 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 1.9e-96
Identity = 176/201 (87.56%), Postives = 185/201 (92.04%), Query Frame = 1 

Query: 22  GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
           G  L  SKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFK
Sbjct: 759 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFK 818

Query: 82  PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
           PSKSHILAAVENFTNLLQIRYSSPLFRLKT+NAIQKR+   FHN+GTSLIPG+IVMSIED
Sbjct: 819 PSKSHILAAVENFTNLLQIRYSSPLFRLKTSNAIQKRV--RFHNSGTSLIPGLIVMSIED 878

Query: 142 GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
           G  GIPGL+QLD  YSYIVVVVNARP+EISFPCP LRA+TLQLHPIQLMSTDP+VKNS Y
Sbjct: 879 GHNGIPGLSQLDSTYSYIVVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTY 938

Query: 202 EPSTGCFMVPSRTTSVFVELR 223
           EPSTGCFMVP RTTSVFVE R
Sbjct: 939 EPSTGCFMVPPRTTSVFVEPR 957

[Close]
BLAST of CmaCh18G009140 vs. TrEMBL
Match: M5XR33_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000905mg PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 5.7e-85
Identity = 155/202 (76.73%), Postives = 175/202 (86.63%), Query Frame = 1 

Query: 21  TGPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSF 80
           +G  +  SKS+DRDSYNSGDWFNRLDFTY +NNWGVGLPPKEKNE +WPL KPRLADPSF
Sbjct: 764 SGDEILRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEKSWPLFKPRLADPSF 823

Query: 81  KPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIE 140
           KP KSHI+AAVENF+NLL+IRYSSPLFRL+TANAIQ+R+   FHNTG SL+PGVIVMSIE
Sbjct: 824 KPQKSHIIAAVENFSNLLRIRYSSPLFRLRTANAIQERV--RFHNTGPSLVPGVIVMSIE 883

Query: 141 DGQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSA 200
           DG EG+PGL+QLDPIYSYIVV+VNA P+E+SF  P L+ARTLQLHP Q+MSTD +VK S 
Sbjct: 884 DGHEGVPGLSQLDPIYSYIVVIVNACPTEVSFASPSLQARTLQLHPEQVMSTDEIVKRST 943

Query: 201 YEPSTGCFMVPSRTTSVFVELR 223
           Y  S GCF VP RTTSVFVE R
Sbjct: 944 YNASAGCFTVPPRTTSVFVEPR 963

[Close]
BLAST of CmaCh18G009140 vs. TrEMBL
Match: D7TM28_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g05160 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 2.8e-84
Identity = 149/202 (73.76%), Postives = 176/202 (87.13%), Query Frame = 1 

Query: 21  TGPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSF 80
           +G  +  SKS+DRDSYNSGDWFNRLDF+Y +NNWGVGLPPKEKNE NWPLIKPRLADPSF
Sbjct: 755 SGDEMLRSKSIDRDSYNSGDWFNRLDFSYNSNNWGVGLPPKEKNEKNWPLIKPRLADPSF 814

Query: 81  KPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIE 140
           KP K+HI+AAVENF NLL+IRYSSPLFRL+TAN+IQ R+   FHNTG S +PG+IVMSIE
Sbjct: 815 KPQKNHIIAAVENFLNLLKIRYSSPLFRLRTANSIQARV--RFHNTGPSWVPGIIVMSIE 874

Query: 141 DGQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSA 200
           DG EG+PGL+QLDP+YSYIVV++N  P+EI F  P L+ARTLQLHP+Q+MS+D ++KNS 
Sbjct: 875 DGHEGVPGLSQLDPVYSYIVVIINPSPTEIKFASPALQARTLQLHPVQVMSSDGVIKNST 934

Query: 201 YEPSTGCFMVPSRTTSVFVELR 223
           YE S+GCF+VP RTTSVFVE R
Sbjct: 935 YEASSGCFIVPPRTTSVFVEPR 954

[Close]
BLAST of CmaCh18G009140 vs. TrEMBL
Match: A0A0D2SB26_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G305300 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.5e-82
Identity = 159/224 (70.98%), Postives = 183/224 (81.70%), Query Frame = 1 

Query: 1   MNYYAQNQWIVIHITPVIG--STGPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGL 60
           MN++A +   +I +T  I     G  +  SKS+DRDSYNSGDWFNRLDFTY +NNWGVGL
Sbjct: 736 MNHFATS---IIALTQGIPFFHAGDEMLRSKSLDRDSYNSGDWFNRLDFTYNSNNWGVGL 795

Query: 61  PPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKR 120
           PPK+KNE +WPLI+PRLADPSFKP +SHILAAVENFT++L IRYSSPLFRL+TANAIQ+R
Sbjct: 796 PPKQKNENSWPLIQPRLADPSFKPQRSHILAAVENFTDVLCIRYSSPLFRLRTANAIQER 855

Query: 121 MGRSFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLR 180
           +   FHNTG S +PG IVMSIEDG EGIPGL+QLDPI+SYIVVV NA P+E SF  P LR
Sbjct: 856 V--RFHNTGPSWVPGFIVMSIEDGNEGIPGLSQLDPIFSYIVVVFNACPTEESFSSPTLR 915

Query: 181 ARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFVELR 223
            RTLQLHPIQ+ STD  VK S+YE STGCF VP+RTTSVFVE R
Sbjct: 916 GRTLQLHPIQVTSTDETVKKSSYEASTGCFTVPARTTSVFVEPR 954

[Close]
BLAST of CmaCh18G009140 vs. TrEMBL
Match: A0A061FPM1_THECC (Limit dextrinase OS=Theobroma cacao GN=TCM_043341 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 3.4e-82
Identity = 150/201 (74.63%), Postives = 173/201 (86.07%), Query Frame = 1 

Query: 22  GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
           G  +  SKS+DRDSYNSGDWFNRLDFTY +NNWGVGLPPKEKNE NWPLI+PRLADPSFK
Sbjct: 759 GDEMLRSKSLDRDSYNSGDWFNRLDFTYHSNNWGVGLPPKEKNEKNWPLIQPRLADPSFK 818

Query: 82  PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
           P +SHILAAVENF N+L+IRYSSPLFRL+TANAIQ+R+   FHNTG S +PG+IVMSIED
Sbjct: 819 PQRSHILAAVENFMNVLRIRYSSPLFRLRTANAIQQRV--RFHNTGPSWVPGLIVMSIED 878

Query: 142 GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
           G EG+PG++QLDP +SYIVV+ NA P+E SF    LR RTLQLHPIQ+MSTD +VKNS+Y
Sbjct: 879 GNEGVPGISQLDPNFSYIVVIFNACPTEASFISSTLRGRTLQLHPIQVMSTDEVVKNSSY 938

Query: 202 EPSTGCFMVPSRTTSVFVELR 223
           E  +GCF VP+RTTSVFVE R
Sbjct: 939 EALSGCFTVPARTTSVFVEAR 957

[Close]
BLAST of CmaCh18G009140 vs. TAIR10
Match: AT5G04360.1 (AT5G04360.1 limit dextrinase)

HSP 1 Score: 288.1 bits (736), Expect = 4.6e-78
Identity = 137/201 (68.16%), Postives = 162/201 (80.60%), Query Frame = 1 

Query: 22  GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
           G  +  SKS+DRDSYNSGDWFNRLDF+Y +NNWGVGLPPK KNE+NWPLIKPRL DPSFK
Sbjct: 765 GDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFK 824

Query: 82  PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
           P  SHI+A + NF +LL+IRYSSPLFRL TA AIQ+R+   FHNTG S IPG IVMSIED
Sbjct: 825 PKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERV--RFHNTGPSSIPGAIVMSIED 884

Query: 142 GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
           G  GIP ++Q+DPIYS IVV+ NARPSE S+P P L+ R L+LHP+Q+MS D +VK S Y
Sbjct: 885 GHRGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVY 944

Query: 202 EPSTGCFMVPSRTTSVFVELR 223
           +  +G F VP+RTT+VFVE R
Sbjct: 945 DSFSGGFTVPARTTTVFVESR 963

[Close]
BLAST of CmaCh18G009140 vs. NCBI nr
Match: gi|449449505|ref|XP_004142505.1| (PREDICTED: pullulanase 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 360.1 bits (923), Expect = 2.7e-96
Identity = 176/201 (87.56%), Postives = 185/201 (92.04%), Query Frame = 1 

Query: 22  GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
           G  L  SKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFK
Sbjct: 759 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFK 818

Query: 82  PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
           PSKSHILAAVENFTNLLQIRYSSPLFRLKT+NAIQKR+   FHN+GTSLIPG+IVMSIED
Sbjct: 819 PSKSHILAAVENFTNLLQIRYSSPLFRLKTSNAIQKRV--RFHNSGTSLIPGLIVMSIED 878

Query: 142 GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
           G  GIPGL+QLD  YSYIVVVVNARP+EISFPCP LRA+TLQLHPIQLMSTDP+VKNS Y
Sbjct: 879 GHNGIPGLSQLDSTYSYIVVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTY 938

Query: 202 EPSTGCFMVPSRTTSVFVELR 223
           EPSTGCFMVP RTTSVFVE R
Sbjct: 939 EPSTGCFMVPPRTTSVFVEPR 957

[Close]
BLAST of CmaCh18G009140 vs. NCBI nr
Match: gi|659091942|ref|XP_008446816.1| (PREDICTED: pullulanase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 353.2 bits (905), Expect = 3.3e-94
Identity = 173/201 (86.07%), Postives = 184/201 (91.54%), Query Frame = 1 

Query: 22  GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
           G  L  SKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFK
Sbjct: 765 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFK 824

Query: 82  PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
           PSKSHILAAVENFTNLLQIRYSS LFRLKT++AIQKR+   FHN+GTSLIPG+IVMSIED
Sbjct: 825 PSKSHILAAVENFTNLLQIRYSSRLFRLKTSHAIQKRV--RFHNSGTSLIPGLIVMSIED 884

Query: 142 GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
           G EGIPGL+QLD  YSYIVVV NARP+EISFPCP LRA+TLQLHPIQLMSTDP+VKNS Y
Sbjct: 885 GHEGIPGLSQLDSTYSYIVVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTY 944

Query: 202 EPSTGCFMVPSRTTSVFVELR 223
           EPSTGCF+VP RTTSVFVE R
Sbjct: 945 EPSTGCFVVPPRTTSVFVEPR 963

[Close]
BLAST of CmaCh18G009140 vs. NCBI nr
Match: gi|694386369|ref|XP_009368969.1| (PREDICTED: pullulanase 1, chloroplastic-like isoform X1 [Pyrus x bretschneideri])

HSP 1 Score: 324.3 bits (830), Expect = 1.6e-85
Identity = 154/201 (76.62%), Postives = 178/201 (88.56%), Query Frame = 1 

Query: 22  GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
           G  L  SKS+DRDSYNSGDWFNRLDFTY +NNWGVGLPPKEKNE++WPL+KPRLADPSFK
Sbjct: 778 GDELLRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEHSWPLMKPRLADPSFK 837

Query: 82  PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
           P KSHILAAVENF+NLL+IRYSSPLFRL+TANAIQ+R+   FHNTG SL+PGVIVMSIED
Sbjct: 838 PQKSHILAAVENFSNLLRIRYSSPLFRLRTANAIQERV--RFHNTGPSLVPGVIVMSIED 897

Query: 142 GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
           G EG+PGL+QLDPI+SYIVV++NA P+E+SF  P LR+RTLQLHP+Q+ STD +VK S Y
Sbjct: 898 GHEGVPGLSQLDPIFSYIVVIINACPTEVSFASPPLRSRTLQLHPVQVTSTDEIVKRSTY 957

Query: 202 EPSTGCFMVPSRTTSVFVELR 223
           + S+GCF VP RTTSVFVE R
Sbjct: 958 DGSSGCFTVPPRTTSVFVEPR 976

[Close]
BLAST of CmaCh18G009140 vs. NCBI nr
Match: gi|694386371|ref|XP_009368970.1| (PREDICTED: pullulanase 1, chloroplastic-like isoform X2 [Pyrus x bretschneideri])

HSP 1 Score: 324.3 bits (830), Expect = 1.6e-85
Identity = 154/201 (76.62%), Postives = 178/201 (88.56%), Query Frame = 1 

Query: 22  GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
           G  L  SKS+DRDSYNSGDWFNRLDFTY +NNWGVGLPPKEKNE++WPL+KPRLADPSFK
Sbjct: 777 GDELLRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEHSWPLMKPRLADPSFK 836

Query: 82  PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
           P KSHILAAVENF+NLL+IRYSSPLFRL+TANAIQ+R+   FHNTG SL+PGVIVMSIED
Sbjct: 837 PQKSHILAAVENFSNLLRIRYSSPLFRLRTANAIQERV--RFHNTGPSLVPGVIVMSIED 896

Query: 142 GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
           G EG+PGL+QLDPI+SYIVV++NA P+E+SF  P LR+RTLQLHP+Q+ STD +VK S Y
Sbjct: 897 GHEGVPGLSQLDPIFSYIVVIINACPTEVSFASPPLRSRTLQLHPVQVTSTDEIVKRSTY 956

Query: 202 EPSTGCFMVPSRTTSVFVELR 223
           + S+GCF VP RTTSVFVE R
Sbjct: 957 DGSSGCFTVPPRTTSVFVEPR 975

[Close]
BLAST of CmaCh18G009140 vs. NCBI nr
Match: gi|694386324|ref|XP_009368949.1| (PREDICTED: pullulanase 1, chloroplastic-like [Pyrus x bretschneideri])

HSP 1 Score: 323.2 bits (827), Expect = 3.6e-85
Identity = 153/201 (76.12%), Postives = 178/201 (88.56%), Query Frame = 1 

Query: 22  GPVLFFSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 81
           G  L  SKS+DRDSYNSGDWFNRLDFTY +NNWGVGLPPKEKNE++WPL+KPRLADPSFK
Sbjct: 777 GDELLRSKSLDRDSYNSGDWFNRLDFTYSSNNWGVGLPPKEKNEHSWPLMKPRLADPSFK 836

Query: 82  PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRMGRSFHNTGTSLIPGVIVMSIED 141
           P KSHI+AAVENF+NLL+IRYSSPLFRL+TANAIQ+R+   FHNTG SL+PGVIVMSIED
Sbjct: 837 PQKSHIVAAVENFSNLLRIRYSSPLFRLRTANAIQERV--RFHNTGPSLVPGVIVMSIED 896

Query: 142 GQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAY 201
           G EG+PGL+QLDPI+SYIVV++NA P+E+SF  P LR+RTLQLHP+Q+ STD +VK S Y
Sbjct: 897 GHEGVPGLSQLDPIFSYIVVIINACPTEVSFASPPLRSRTLQLHPVQVTSTDEIVKRSTY 956

Query: 202 EPSTGCFMVPSRTTSVFVELR 223
           + S+GCF VP RTTSVFVE R
Sbjct: 957 DGSSGCFTVPPRTTSVFVEPR 975

The following BLAST results are available for this feature:
BLAST of CmaCh18G009140 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PULA1_ARATH8.1e-7768.16Pullulanase 1, chloroplastic OS=Arabidopsis thaliana GN=PU1 PE=2 SV=2[more]
PULA_KLEPN1.4e-2032.65Pullulanase OS=Klebsiella pneumoniae GN=pulA PE=1 SV=2[more]
PULA_ENTAE4.2e-1738.21Pullulanase OS=Enterobacter aerogenes GN=pulA PE=1 SV=1[more]
BLAST of CmaCh18G009140 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KTI2_CUCSA1.9e-9687.56Uncharacterized protein OS=Cucumis sativus GN=Csa_5G622510 PE=4 SV=1[more]
M5XR33_PRUPE5.7e-8576.73Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000905mg PE=4 SV=1[more]
D7TM28_VITVI2.8e-8473.76Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g05160 PE=4 SV=... [more]
A0A0D2SB26_GOSRA1.5e-8270.98Uncharacterized protein OS=Gossypium raimondii GN=B456_009G305300 PE=4 SV=1[more]
A0A061FPM1_THECC3.4e-8274.63Limit dextrinase OS=Theobroma cacao GN=TCM_043341 PE=4 SV=1[more]
BLAST of CmaCh18G009140 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G04360.14.6e-7868.16 limit dextrinase[more]
BLAST of CmaCh18G009140 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449449505|ref|XP_004142505.1|2.7e-9687.56PREDICTED: pullulanase 1, chloroplastic [Cucumis sativus][more]
gi|659091942|ref|XP_008446816.1|3.3e-9486.07PREDICTED: pullulanase 1, chloroplastic [Cucumis melo][more]
gi|694386369|ref|XP_009368969.1|1.6e-8576.62PREDICTED: pullulanase 1, chloroplastic-like isoform X1 [Pyrus x bretschneideri][more]
gi|694386371|ref|XP_009368970.1|1.6e-8576.62PREDICTED: pullulanase 1, chloroplastic-like isoform X2 [Pyrus x bretschneideri][more]
gi|694386324|ref|XP_009368949.1|3.6e-8576.12PREDICTED: pullulanase 1, chloroplastic-like [Pyrus x bretschneideri][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR015902Glycoside hydrolase, family 13
IPR017853Glycoside_hydrolase_SF
IPR024561Pullul_strch_C
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015996 chlorophyll catabolic process
biological_process GO:0019761 glucosinolate biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process GO:0000023 maltose metabolic process
biological_process GO:0016556 mRNA modification
biological_process GO:0006098 pentose-phosphate shunt
biological_process GO:0009664 plant-type cell wall organization
biological_process GO:0019252 starch biosynthetic process
biological_process GO:0005983 starch catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010303 limit dextrinase activity
molecular_function GO:0051060 pullulanase activity
molecular_function GO:0003824 catalytic activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G009140.1CmaCh18G009140.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015902Glycoside hydrolase, family 13PANTHERPTHR10357ALPHA-AMYLASEcoord: 28..62
score: 3.4E-52coord: 89..220
score: 3.4
IPR017853Glycoside hydrolase superfamilyunknownSSF51445(Trans)glycosidasescoord: 26..108
score: 2.9
IPR024561Alpha-1,6-glucosidases, pullulanase-type, C-terminalPFAMPF11852DUF3372coord: 50..220
score: 1.4
NoneNo IPR availablePANTHERPTHR10357:SF99PULLULANASE 1, CHLOROPLASTICcoord: 89..220
score: 3.4E-52coord: 28..62
score: 3.4
NoneNo IPR availableunknownSSF51011Glycosyl hydrolase domaincoord: 111..222
score: 2.09

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh18G009140Melon (DHL92) v3.6.1cmamedB408
CmaCh18G009140Melon (DHL92) v3.6.1cmamedB431
CmaCh18G009140Silver-seed gourdcarcmaB0672
CmaCh18G009140Silver-seed gourdcarcmaB0964
CmaCh18G009140Silver-seed gourdcarcmaB1167
CmaCh18G009140Cucumber (Chinese Long) v3cmacucB0498
CmaCh18G009140Watermelon (97103) v2cmawmbB423
CmaCh18G009140Watermelon (97103) v2cmawmbB435
CmaCh18G009140Wax gourdcmawgoB0512
CmaCh18G009140Wax gourdcmawgoB0544
CmaCh18G009140Cucurbita maxima (Rimu)cmacmaB402
CmaCh18G009140Cucumber (Gy14) v1cgycmaB0849
CmaCh18G009140Cucurbita moschata (Rifu)cmacmoB406
CmaCh18G009140Cucurbita moschata (Rifu)cmacmoB420
CmaCh18G009140Wild cucumber (PI 183967)cmacpiB423
CmaCh18G009140Cucumber (Chinese Long) v2cmacuB417
CmaCh18G009140Melon (DHL92) v3.5.1cmameB351
CmaCh18G009140Melon (DHL92) v3.5.1cmameB374
CmaCh18G009140Watermelon (Charleston Gray)cmawcgB364
CmaCh18G009140Watermelon (Charleston Gray)cmawcgB373
CmaCh18G009140Watermelon (97103) v1cmawmB365
CmaCh18G009140Watermelon (97103) v1cmawmB372
CmaCh18G009140Cucurbita pepo (Zucchini)cmacpeB415
CmaCh18G009140Cucurbita pepo (Zucchini)cmacpeB429
CmaCh18G009140Cucurbita pepo (Zucchini)cmacpeB435
CmaCh18G009140Bottle gourd (USVL1VR-Ls)cmalsiB379
CmaCh18G009140Bottle gourd (USVL1VR-Ls)cmalsiB395
CmaCh18G009140Cucumber (Gy14) v2cgybcmaB626
CmaCh18G009140Cucumber (Gy14) v2cgybcmaB770