CmaCh18G000350 (gene) Cucurbita maxima (Rimu)

NameCmaCh18G000350
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionEukaryotic aspartyl protease family protein
LocationCma_Chr18 : 135761 .. 137239 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCTGCAAAGCCAAATCAGCTACCTTTCTGTCCTTTCGCCATCTTCCTCTTCCTCTTCCTCTTCCTCTCTCTCTTCCCCTTCACTCTCTCCTCCTCCGCTTCACTCGATGTTTCTACTTCTCTGAAACAAGCCCACCAGGTCCTTAAATTCGACCCTGAGATTTTGACCTCTTTCCACCAGCAGGAACTGCTTCTTCCGGCGAACTCTTCTTCTTCCTCCTCCTTCTCTCTGCAGCTGCACCCTCGTGATGCTCTTCGCAATGCCGGACATACGGACTACAGGAGCTTGGTCCTCTCCCGGCTCGACCGTGACTCGTCTAGAGTTAAATCGCTTAATGATAGGCTTGAATTTGGCTTAAGCGACTTGACTAGGTCGGATCTTCAGCCGTTGAACGCGGACATTCTGCCGGAGGATCTGAGCACTCCCATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTTGGCGTTGGTCAACCGGCCAAGCCATTCTATATGGTTCTGGATACCGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCCATTTTTGACCCTACCACTTCGTCTTCTTTTACTTCCCTTCCCTGCGAATCTCCGCACTGTCAAGCTCTTGAAATGTCTGGTTGTCGTTCTGCGAAGTGTCTTTACCAGGTCTCGTACGGCGATGGCTCCTTCACCGTCGGCCAGTTCGTCACTGAAACGCTGTCGTTTGGTAACTCCGGAGCGATTAACAACGTCGCTCTCGGCTGTGGCCACGACAACGAAGGACTGTTCATCGGATCCGCCGGATTGCTTGGTCTCGGCGGCGGATCACTTTCCCTAACTTCTCAGATGAAAGCTTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCAGCATCAACTCTCGAGTTCAATTCCGCTGCACCGAATGACTCGGTGAATGCTCAATTACTCAGAAGCGGAAGAGTCAATACATTCTACTACGTTGCACTCACCGGCTTGAGCGTCGGCGGCCATCCACTCTCTATTCCGCCTTCTCTGTTTCAATTGGACGATTCCGGCCACGGCGGCATCATCGTAGATTCAGGAACCGCCATAACTAGACTGCAGACTCAGGCCTACAACTCCCTTCGCGACGCCTTCGTGAGGCTCACACCGTACTTGAAGAAGGCGAACAGCTTCGCATTATTCGACACATGTTACGATCTGTCGTCGCAATCGAGAGTGACGATTCCGACAGTATCTTTTCATTTCTCGGGCGGAAAGTCGCTGCAGCTGGCGCCGAAGAACTACCTCATTCCGGTGGATTCAGCCGGAACTTTCTGTTTTGCGTTCGCCCCAACGACGTCGTCGCTGTCCATCATAGGGAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGACTTGGCTAATTCGGTGGTAGCATTTTCACCCAATAAATGTTAG

mRNA sequence

ATGGCGTCTGCAAAGCCAAATCAGCTACCTTTCTGTCCTTTCGCCATCTTCCTCTTCCTCTTCCTCTTCCTCTCTCTCTTCCCCTTCACTCTCTCCTCCTCCGCTTCACTCGATGTTTCTACTTCTCTGAAACAAGCCCACCAGGTCCTTAAATTCGACCCTGAGATTTTGACCTCTTTCCACCAGCAGGAACTGCTTCTTCCGGCGAACTCTTCTTCTTCCTCCTCCTTCTCTCTGCAGCTGCACCCTCGTGATGCTCTTCGCAATGCCGGACATACGGACTACAGGAGCTTGGTCCTCTCCCGGCTCGACCGTGACTCGTCTAGAGTTAAATCGCTTAATGATAGGCTTGAATTTGGCTTAAGCGACTTGACTAGGTCGGATCTTCAGCCGTTGAACGCGGACATTCTGCCGGAGGATCTGAGCACTCCCATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTTGGCGTTGGTCAACCGGCCAAGCCATTCTATATGGTTCTGGATACCGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCCATTTTTGACCCTACCACTTCGTCTTCTTTTACTTCCCTTCCCTGCGAATCTCCGCACTGTCAAGCTCTTGAAATGTCTGGTTGTCGTTCTGCGAAGTGTCTTTACCAGGTCTCGTACGGCGATGGCTCCTTCACCGTCGGCCAGTTCGTCACTGAAACGCTGTCGTTTGGTAACTCCGGAGCGATTAACAACGTCGCTCTCGGCTGTGGCCACGACAACGAAGGACTGTTCATCGGATCCGCCGGATTGCTTGGTCTCGGCGGCGGATCACTTTCCCTAACTTCTCAGATGAAAGCTTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCAGCATCAACTCTCGAGTTCAATTCCGCTGCACCGAATGACTCGGTGAATGCTCAATTACTCAGAAGCGGAAGAGTCAATACATTCTACTACGTTGCACTCACCGGCTTGAGCGTCGGCGGCCATCCACTCTCTATTCCGCCTTCTCTGTTTCAATTGGACGATTCCGGCCACGGCGGCATCATCGTAGATTCAGGAACCGCCATAACTAGACTGCAGACTCAGGCCTACAACTCCCTTCGCGACGCCTTCGTGAGGCTCACACCGTACTTGAAGAAGGCGAACAGCTTCGCATTATTCGACACATGTTACGATCTGTCGTCGCAATCGAGAGTGACGATTCCGACAGTATCTTTTCATTTCTCGGGCGGAAAGTCGCTGCAGCTGGCGCCGAAGAACTACCTCATTCCGGTGGATTCAGCCGGAACTTTCTGTTTTGCGTTCGCCCCAACGACGTCGTCGCTGTCCATCATAGGGAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGACTTGGCTAATTCGGTGGTAGCATTTTCACCCAATAAATGTTAG

Coding sequence (CDS)

ATGGCGTCTGCAAAGCCAAATCAGCTACCTTTCTGTCCTTTCGCCATCTTCCTCTTCCTCTTCCTCTTCCTCTCTCTCTTCCCCTTCACTCTCTCCTCCTCCGCTTCACTCGATGTTTCTACTTCTCTGAAACAAGCCCACCAGGTCCTTAAATTCGACCCTGAGATTTTGACCTCTTTCCACCAGCAGGAACTGCTTCTTCCGGCGAACTCTTCTTCTTCCTCCTCCTTCTCTCTGCAGCTGCACCCTCGTGATGCTCTTCGCAATGCCGGACATACGGACTACAGGAGCTTGGTCCTCTCCCGGCTCGACCGTGACTCGTCTAGAGTTAAATCGCTTAATGATAGGCTTGAATTTGGCTTAAGCGACTTGACTAGGTCGGATCTTCAGCCGTTGAACGCGGACATTCTGCCGGAGGATCTGAGCACTCCCATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTTGGCGTTGGTCAACCGGCCAAGCCATTCTATATGGTTCTGGATACCGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCCATTTTTGACCCTACCACTTCGTCTTCTTTTACTTCCCTTCCCTGCGAATCTCCGCACTGTCAAGCTCTTGAAATGTCTGGTTGTCGTTCTGCGAAGTGTCTTTACCAGGTCTCGTACGGCGATGGCTCCTTCACCGTCGGCCAGTTCGTCACTGAAACGCTGTCGTTTGGTAACTCCGGAGCGATTAACAACGTCGCTCTCGGCTGTGGCCACGACAACGAAGGACTGTTCATCGGATCCGCCGGATTGCTTGGTCTCGGCGGCGGATCACTTTCCCTAACTTCTCAGATGAAAGCTTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCAGCATCAACTCTCGAGTTCAATTCCGCTGCACCGAATGACTCGGTGAATGCTCAATTACTCAGAAGCGGAAGAGTCAATACATTCTACTACGTTGCACTCACCGGCTTGAGCGTCGGCGGCCATCCACTCTCTATTCCGCCTTCTCTGTTTCAATTGGACGATTCCGGCCACGGCGGCATCATCGTAGATTCAGGAACCGCCATAACTAGACTGCAGACTCAGGCCTACAACTCCCTTCGCGACGCCTTCGTGAGGCTCACACCGTACTTGAAGAAGGCGAACAGCTTCGCATTATTCGACACATGTTACGATCTGTCGTCGCAATCGAGAGTGACGATTCCGACAGTATCTTTTCATTTCTCGGGCGGAAAGTCGCTGCAGCTGGCGCCGAAGAACTACCTCATTCCGGTGGATTCAGCCGGAACTTTCTGTTTTGCGTTCGCCCCAACGACGTCGTCGCTGTCCATCATAGGGAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGACTTGGCTAATTCGGTGGTAGCATTTTCACCCAATAAATGTTAG

Protein sequence

MASAKPNQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC
BLAST of CmaCh18G000350 vs. Swiss-Prot
Match: ASPG1_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 2.5e-163
Identity = 298/497 (59.96%), Postives = 373/497 (75.05%), Query Frame = 1

Query: 13  PFAIFLFLFLFLSLFPFTLSSSAS----------LDVSTSLKQAHQVLKFDP--EILTSF 72
           P  + L   + LSLF  T  +S+           LDV +SL+Q   +L  DP    LT+ 
Sbjct: 4   PRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTT 63

Query: 73  HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG 132
             + L  P   +SSS  SL+LH RD    + H DY+SL LSRL+RDSSRV  +  ++ F 
Sbjct: 64  KPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFA 123

Query: 133 LSDLTRSDLQPL-NADIL--PEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGS 192
           +  + RSDL+P+ N D     EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTGS
Sbjct: 124 VEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGS 183

Query: 193 DINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGD 252
           D+NW+QC+PC DCYQQ+DP+F+PT+SS++ SL C +P C  LE S CRS KCLYQVSYGD
Sbjct: 184 DVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGD 243

Query: 253 GSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASS 312
           GSFTVG+  T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+S
Sbjct: 244 GSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATS 303

Query: 313 FSYCLVDRDSGSASTLEFNSA-APNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPP 372
           FSYCLVDRDSG +S+L+FNS         A LLR+ +++TFYYV L+G SVGG  + +P 
Sbjct: 304 FSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPD 363

Query: 373 SLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKK-ANSFALFDTCYDLS 432
           ++F +D SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  LKK ++S +LFDTCYD S
Sbjct: 364 AIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFS 423

Query: 433 SQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 492
           S S V +PTV+FHF+GGKSL L  KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGTR
Sbjct: 424 SLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTR 483

BLAST of CmaCh18G000350 vs. Swiss-Prot
Match: APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 9.5e-110
Identity = 240/488 (49.18%), Postives = 307/488 (62.91%), Query Frame = 1

Query: 16  IFLFLFLFLSLFPFT-LSSSASLDVSTSLKQAHQVLKFDPEILT-SFHQQELLLPANSSS 75
           +F   F FLSL  F+ L S  +L  ++        + F P+  + S  + E    ++S S
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSES 68

Query: 76  SSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND-RLEFGLSDLTRSDLQPL 135
           SSS +L L   DAL +    D   L  SRL RDS RVKS+     +    ++T +     
Sbjct: 69  SSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPR--- 128

Query: 136 NADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 195
                P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC  CY 
Sbjct: 129 -----PGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYS 188

Query: 196 QTDPIFDPTTSSSFTSLPCESPHCQALEMSGC--RSAKCLYQVSYGDGSFTVGQFVTETL 255
           Q+DPIFDP  S ++ ++PC SPHC+ L+ +GC  R   CLYQVSYGDGSFTVG F TETL
Sbjct: 189 QSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETL 248

Query: 256 SFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSL---TSQMKASSFSYCLVDRDS 315
           +F     +  VALGCGHDNEGLF+G+AGLLGLG G LS    T       FSYCLVDR +
Sbjct: 249 TF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSA 308

Query: 316 GS--ASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL-SIPPSLFQLDDS 375
            S  +S +  N+A    +    LL + +++TFYYV L G+SVGG  +  +  SLF+LD  
Sbjct: 309 SSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQI 368

Query: 376 GHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPT 435
           G+GG+I+DSGT++TRL   AY ++RDAF      LK+A  F+LFDTC+DLS+ + V +PT
Sbjct: 369 GNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPT 428

Query: 436 VSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSV 493
           V  HF G   + L   NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +DLA+S 
Sbjct: 429 VVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSR 484

BLAST of CmaCh18G000350 vs. Swiss-Prot
Match: ASPG2_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 2.9e-103
Identity = 206/480 (42.92%), Postives = 288/480 (60.00%), Query Frame = 1

Query: 17  FLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPANSSSSSS 76
           F FL L L L   +  S     +   L+    V    P+   +    E        SSS 
Sbjct: 7   FFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDE--------SSSK 66

Query: 77  FSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQPLNADI 136
           ++L+L  RD   +  + ++   + +R+ RD+ RV ++  R+   +   + S  +      
Sbjct: 67  YTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEV----- 126

Query: 137 LPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDP 196
              D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  CY+Q+DP
Sbjct: 127 --NDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDP 186

Query: 197 IFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSG 256
           +FDP  S S+T + C S  C  +E SGC S  C Y+V YGDGS+T G    ETL+F  + 
Sbjct: 187 VFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKT- 246

Query: 257 AINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASS---FSYCLVDRDSGSASTL 316
            + NVA+GCGH N G+FIG+AGLLG+GGGS+S   Q+   +   F YCLV R + S  +L
Sbjct: 247 VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSL 306

Query: 317 EFN-SAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVD 376
            F   A P  +    L+R+ R  +FYYV L GL VGG  + +P  +F L ++G GG+++D
Sbjct: 307 VFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMD 366

Query: 377 SGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSGG 436
           +GTA+TRL T AY + RD F   T  L +A+  ++FDTCYDLS    V +PTVSF+F+ G
Sbjct: 367 TGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEG 426

Query: 437 KSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
             L L  +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  V F PN C
Sbjct: 427 PVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of CmaCh18G000350 vs. Swiss-Prot
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.7e-74
Identity = 156/358 (43.58%), Postives = 206/358 (57.54%), Query Frame = 1

Query: 141 LSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDP 200
           + TP+ +G     GEY   V +G P   F  ++DTGSD+ W QC+PCT C+ Q  PIF+P
Sbjct: 85  IETPVYAGD----GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNP 144

Query: 201 TTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSGAINN 260
             SSSF++LPCES +CQ L    C + +C Y   YGDGS T G   TET +F  S ++ N
Sbjct: 145 QDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPN 204

Query: 261 VALGCGHDNEGLFIGS-AGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAA 320
           +A GCG DN+G   G+ AGL+G+G G LSL SQ+    FSYC+    S S STL   SAA
Sbjct: 205 IAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAA 264

Query: 321 ---PNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVDSGTA 380
              P  S +  L+ S    T+YY+ L G++VGG  L IP S FQL D G GG+I+DSGT 
Sbjct: 265 SGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTT 324

Query: 381 ITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQ-SRVTIPTVSFHFSGGKSL 440
           +T L   AYN++  AF           S +   TC+   S  S V +P +S  F GG  L
Sbjct: 325 LTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VL 384

Query: 441 QLAPKNYLIPVDSAGTFCFAFAPTTS-SLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
            L  +N LI   + G  C A   ++   +SI GN+QQQ T+V +DL N  V+F P +C
Sbjct: 385 NLGEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of CmaCh18G000350 vs. Swiss-Prot
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 6.4e-74
Identity = 156/347 (44.96%), Postives = 206/347 (59.37%), Query Frame = 1

Query: 152 GSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPC 211
           G GEY   + +G PA+PF  ++DTGSD+ W QCQPCT C+ Q+ PIF+P  SSSF++LPC
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 150

Query: 212 ESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSGAINNVALGCGHDNEG 271
            S  CQAL    C +  C Y   YGDGS T G   TETL+FG S +I N+  GCG +N+G
Sbjct: 151 SSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQG 210

Query: 272 LFIGS-AGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAAPN---DSVNAQ 331
              G+ AGL+G+G G LSL SQ+  + FSYC+    S + S L   S A +    S N  
Sbjct: 211 FGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTT 270

Query: 332 LLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLD-DSGHGGIIVDSGTAITRLQTQAYN 391
           L++S ++ TFYY+ L GLSVG   L I PS F L+ ++G GGII+DSGT +T     AY 
Sbjct: 271 LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQ 330

Query: 392 SLRDAFVRLTPYLKKANSFALFDTCYDL-SSQSRVTIPTVSFHFSGGKSLQLAPKNYLIP 451
           S+R  F+          S + FD C+   S  S + IPT   HF GG  L+L  +NY I 
Sbjct: 331 SVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS 390

Query: 452 VDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
             S G  C A   ++  +SI GN+QQQ   V +D  NSVV+F+  +C
Sbjct: 391 -PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of CmaCh18G000350 vs. TrEMBL
Match: A0A0A0LQD2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G042220 PE=3 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 2.3e-224
Identity = 402/486 (82.72%), Postives = 436/486 (89.71%), Query Frame = 1

Query: 16  IFLFLFLFLSLFPFTLS---------SSASLDVSTSLKQAHQVLKFDPEILTSFHQQELL 75
           +FLFLF FLSLFPFTLS         SSASLDVS SL+QA+QVLKFDP    SF QQ  L
Sbjct: 9   LFLFLF-FLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQQVHL 68

Query: 76  LPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTR 135
           +P+NSS   SFSLQLHPRD+L NAGH DY+SLVLSRL RDSSRVKS+ DRLEF LS+L R
Sbjct: 69  VPSNSSF--SFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSELKR 128

Query: 136 SDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQP 195
           SDL+PL  +ILPEDLSTPI+SG+SQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQP
Sbjct: 129 SDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQP 188

Query: 196 CTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFV 255
           CTDCYQQTDPIFDP +SSSF SLPCES  CQALE SGCR++KCLYQVSYGDGSFTVG+FV
Sbjct: 189 CTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGEFV 248

Query: 256 TETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRD 315
            ETL+FGNSG INNVA+GCGHDNEGLF+GSAGLLGLGGGSLSLTSQMKASSFSYCLVDRD
Sbjct: 249 IETLTFGNSGMINNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRD 308

Query: 316 SGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGH 375
           S S+S LEFNSAAP+DSVNA LL+SG+V+TFYYV LTG+SVGG  LSIPP+LFQ+DDSG+
Sbjct: 309 SSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDSGY 368

Query: 376 GGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVS 435
           GGIIVDSGTAITRLQTQAYN+LRDAFV  TPYLKK N FALFDTCYDLSSQSRVTIPTVS
Sbjct: 369 GGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPTVS 428

Query: 436 FHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVA 493
           F F+GGKSLQL PKNYLIPVDS GTFCFAFAPTTSSLSIIGNVQQQGTRV++DLANSVV 
Sbjct: 429 FEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSVVG 488

BLAST of CmaCh18G000350 vs. TrEMBL
Match: M5WPB5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004726mg PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 1.2e-183
Identity = 331/487 (67.97%), Postives = 394/487 (80.90%), Query Frame = 1

Query: 14  FAIFLFLFLFLSLFPFTLSSSAS------LDVSTSLKQAHQVLKFDPEILTSFHQQELLL 73
           +   L  F   SLFP T S S S      LDVS SL QAH VL F+P+ L    +QE   
Sbjct: 8   YLAILSAFTLTSLFPSTHSRSLSEETTTLLDVSASLTQAHDVLSFNPQTLKPLDRQETQA 67

Query: 74  PANSSS--SSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLT 133
            A++ +  +SSFSLQL PRDAL N+ H DY SLV SRL RDS+RV SL+ +L+  + ++ 
Sbjct: 68  QAHTLTPLNSSFSLQLLPRDALHNSQHKDYESLVQSRLGRDSARVNSLHTKLQLVVQNIK 127

Query: 134 RSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ 193
           +SDL+P++ +I PEDLSTP+VSG SQGSGEYF+R+GVG PAK  YMVLDTGSDINWLQC+
Sbjct: 128 KSDLEPMHTEIRPEDLSTPVVSGVSQGSGEYFTRIGVGTPAKSLYMVLDTGSDINWLQCE 187

Query: 194 PCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQF 253
           PC+DCYQQTDP+F+PT SS++  + C+S  C +L +S CR+ KCLYQVSYGDGS+TVG F
Sbjct: 188 PCSDCYQQTDPVFNPTGSSTYRPVTCDSAQCHSLHVSACRADKCLYQVSYGDGSYTVGDF 247

Query: 254 VTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDR 313
           VTET+SFGNSGAI+NV LGCGHDNEGLF+G+AGLLGLGGG+LSL SQ KA+SFSYCLV+R
Sbjct: 248 VTETVSFGNSGAIHNVGLGCGHDNEGLFVGAAGLLGLGGGALSLPSQFKATSFSYCLVNR 307

Query: 314 DSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSG 373
           DS ++STLEFNSA P+DSV A LL+  RV TFYYV L G SVGG P+S+PPS+F++D+SG
Sbjct: 308 DSSTSSTLEFNSAPPSDSVTAPLLKDSRVETFYYVGLKGFSVGGQPVSVPPSVFEVDESG 367

Query: 374 HGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTV 433
           +GGIIVDSGTAITRLQT+AYNSLRDAF RLT  L  A+ FALFDTCYDLSS+SRV +PTV
Sbjct: 368 NGGIIVDSGTAITRLQTEAYNSLRDAFKRLTRDLPSASGFALFDTCYDLSSRSRVQVPTV 427

Query: 434 SFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVV 493
           SF F+ GKSL L  KNYLIPVDSAGTFCFAFAPT+SS SIIGNVQQQGTRV++DLAN+ V
Sbjct: 428 SFLFADGKSLSLPAKNYLIPVDSAGTFCFAFAPTSSSPSIIGNVQQQGTRVSYDLANNRV 487

BLAST of CmaCh18G000350 vs. TrEMBL
Match: A0A0R0IA82_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_09G187200 PE=3 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.3e-182
Identity = 328/478 (68.62%), Postives = 388/478 (81.17%), Query Frame = 1

Query: 16  IFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPANSSSSS 75
           IF  L L  S    T S +  LDVS+SL QAHQ+L F+P++L     +     + SSSSS
Sbjct: 16  IFTTLSLTTSRTITTPSHTNVLDVSSSLHQAHQILSFNPQLLEEQSSETETPTSPSSSSS 75

Query: 76  SFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQPLNAD 135
           SFSLQLHPR+ L N  H +Y++LVLSRL RD++RV SLN +L+  LS L RSDL P   +
Sbjct: 76  SFSLQLHPRETLLNEQHPNYKTLVLSRLARDTARVNSLNTKLQLALSSLNRSDLYPTETE 135

Query: 136 IL-PEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQT 195
           +L PEDLSTP+ SG++QGSGEYFSRVGVGQP+KPFYMVLDTGSD+NWLQC+PC+DCYQQ+
Sbjct: 136 LLRPEDLSTPVSSGTAQGSGEYFSRVGVGQPSKPFYMVLDTGSDVNWLQCKPCSDCYQQS 195

Query: 196 DPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGN 255
           DPIFDPT SSS+  L C++  CQ LEMS CR+ KCLYQVSYGDGSFTVG++VTET+SFG 
Sbjct: 196 DPIFDPTASSSYNPLTCDAQQCQDLEMSACRNGKCLYQVSYGDGSFTVGEYVTETVSFG- 255

Query: 256 SGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLE 315
           +G++N VA+GCGHDNEGLF+GSAGLLGLGGG LSLTSQ+KA+SFSYCLVDRDSG +STLE
Sbjct: 256 AGSVNRVAIGCGHDNEGLFVGSAGLLGLGGGPLSLTSQIKATSFSYCLVDRDSGKSSTLE 315

Query: 316 FNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVDSG 375
           FNS  P DSV A LL++ +VNTFYYV LTG+SVGG  +++PP  F +D SG GG+IVDSG
Sbjct: 316 FNSPRPGDSVVAPLLKNQKVNTFYYVELTGVSVGGEIVTVPPETFAVDQSGAGGVIVDSG 375

Query: 376 TAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSGGKS 435
           TAITRL+TQAYNS+RDAF R T  L+ A   ALFDTCYDLSS   V +PTVSFHFSG ++
Sbjct: 376 TAITRLRTQAYNSVRDAFKRKTSNLRPAEGVALFDTCYDLSSLQSVRVPTVSFHFSGDRA 435

Query: 436 LQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
             L  KNYLIPVD AGT+CFAFAPTTSS+SIIGNVQQQGTRV+FDLANS+V FSPNKC
Sbjct: 436 WALPAKNYLIPVDGAGTYCFAFAPTTSSMSIIGNVQQQGTRVSFDLANSLVGFSPNKC 492

BLAST of CmaCh18G000350 vs. TrEMBL
Match: A0A061FUN2_THECC (Eukaryotic aspartyl protease family protein OS=Theobroma cacao GN=TCM_012281 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 7.1e-181
Identity = 324/482 (67.22%), Postives = 392/482 (81.33%), Query Frame = 1

Query: 17  FLFLFLFLSLFPFTLS-----SSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPA-N 76
           FLF F    LF  TLS     ++ +LDVS+SL+QA  +L FDP+ LTSF Q +   P  N
Sbjct: 11  FLFFFSLSFLFTSTLSRHSPQATTTLDVSSSLEQAQHILSFDPQSLTSFVQAQSAAPFFN 70

Query: 77  SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQ 136
           SSSSS FS+ LH R +LR   HTDY+SLVLSRLDRDSSRV SL  +L+  L+ + +S+L+
Sbjct: 71  SSSSSVFSIPLHSRGSLRKTHHTDYKSLVLSRLDRDSSRVDSLTTKLQLALNGVKKSELR 130

Query: 137 PLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDC 196
           PL  +I PE LSTP++SG+SQGSGEYFSR+GVG P+K FYMVLDTGSDI W+QC+PC+DC
Sbjct: 131 PLPTEIRPEALSTPVISGTSQGSGEYFSRIGVGNPSKQFYMVLDTGSDITWIQCEPCSDC 190

Query: 197 YQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETL 256
           YQQ+DPIF+P+ SS+++ + C S  C +LE+S CRS KCLYQVSYGDGS+TVG+FVTET+
Sbjct: 191 YQQSDPIFNPSGSSTYSPVSCGSRQCSSLEVSACRSGKCLYQVSYGDGSYTVGEFVTETV 250

Query: 257 SFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSA 316
           SFGNSG IN VALGCGHDNEGLF+G+AGLLGLGGG LSLTSQ+KA+SFSYCLV+RDS S+
Sbjct: 251 SFGNSGNINGVALGCGHDNEGLFVGAAGLLGLGGGPLSLTSQIKATSFSYCLVNRDSASS 310

Query: 317 STLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGII 376
           STL+FNS   +DSV A LLR+ +++TFYYV LTG SVGG P+ +P  LF +D SG GG+I
Sbjct: 311 STLDFNSGLTSDSVTAPLLRNQKIDTFYYVGLTGFSVGGQPVQLPTGLFDMDASGKGGVI 370

Query: 377 VDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFS 436
           VD GTA+TRLQTQAYN+LRDAFV+LT  L  A+  ALFDTCYDLSS++ V +PTV+FHF 
Sbjct: 371 VDCGTAVTRLQTQAYNALRDAFVKLTHDLPTASGVALFDTCYDLSSRTSVRVPTVAFHF- 430

Query: 437 GGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPN 493
           GG+SL L  KNYLIPVDS+G FCFAFAPT+SSLSIIGNVQQQGTRV+FDLAN+ V FSP 
Sbjct: 431 GGQSLDLPAKNYLIPVDSSGVFCFAFAPTSSSLSIIGNVQQQGTRVSFDLANNKVGFSPR 490

BLAST of CmaCh18G000350 vs. TrEMBL
Match: V7C608_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_004G160200g PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 6.0e-180
Identity = 327/484 (67.56%), Postives = 385/484 (79.55%), Query Frame = 1

Query: 9   LPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLP 68
           L  C F  F  L L  S    T S +  L++S+SL QA Q+L F P+        +   P
Sbjct: 6   LSLCIF--FTVLSLTSSFTITTPSHTTILNISSSLHQARQILSFKPQQAFQQTLTQKQTP 65

Query: 69  ANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSD 128
             S SSS FSLQLH R+ L NA H DY++LVLSRL RDS+RV SL  +L+  L+ + RS+
Sbjct: 66  T-SRSSSFFSLQLHTRETLFNAQHPDYKTLVLSRLARDSARVNSLETKLQLALNSVKRSE 125

Query: 129 LQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCT 188
           L P   +I PEDLSTP+ SG+SQGSGEYFSRVGVGQPAKPF+MVLDTGSD+NWLQC+PC+
Sbjct: 126 LYPTQTEIRPEDLSTPVSSGTSQGSGEYFSRVGVGQPAKPFFMVLDTGSDVNWLQCKPCS 185

Query: 189 DCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTE 248
           DCYQQTDP+FDPT+SSS+  L C++  CQ LEMS CR+ +CLYQV+YGDGSFTVG++VTE
Sbjct: 186 DCYQQTDPVFDPTSSSSYYPLTCDAQQCQDLEMSACRNGRCLYQVAYGDGSFTVGEYVTE 245

Query: 249 TLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSG 308
           T+SFG SG++N VA+GCGHDNEGLFIG+AGLLGLGGG LSLTSQ+KA+SFSYCLVDRDSG
Sbjct: 246 TVSFGGSGSVNRVAMGCGHDNEGLFIGAAGLLGLGGGPLSLTSQIKAASFSYCLVDRDSG 305

Query: 309 SASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGG 368
            +STLEFNS  P DSV A LL++ +VNTFYYV LTG SVGG  +SIPP  F +D SG GG
Sbjct: 306 KSSTLEFNSPRPGDSVTAPLLKNQKVNTFYYVELTGFSVGGDIVSIPPETFAVDQSGVGG 365

Query: 369 IIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFH 428
           +IVDSGTAITRL+TQAYN++RDAF R+T  L+ A+  ALFDTCYDLSS   V +PTVSFH
Sbjct: 366 VIVDSGTAITRLRTQAYNAVRDAFKRMTQNLRPADGVALFDTCYDLSSLQSVRVPTVSFH 425

Query: 429 FSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFS 488
           F+G K+  L  KNYLIPVDSAGT+CFAFAPTTSSLSIIGNVQQQGTRV+FDLANSVV FS
Sbjct: 426 FAGDKTWALPAKNYLIPVDSAGTYCFAFAPTTSSLSIIGNVQQQGTRVSFDLANSVVGFS 485

Query: 489 PNKC 493
           PNKC
Sbjct: 486 PNKC 486

BLAST of CmaCh18G000350 vs. TAIR10
Match: AT3G18490.1 (AT3G18490.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-164
Identity = 298/497 (59.96%), Postives = 373/497 (75.05%), Query Frame = 1

Query: 13  PFAIFLFLFLFLSLFPFTLSSSAS----------LDVSTSLKQAHQVLKFDP--EILTSF 72
           P  + L   + LSLF  T  +S+           LDV +SL+Q   +L  DP    LT+ 
Sbjct: 4   PRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTT 63

Query: 73  HQQELLLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFG 132
             + L  P   +SSS  SL+LH RD    + H DY+SL LSRL+RDSSRV  +  ++ F 
Sbjct: 64  KPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFA 123

Query: 133 LSDLTRSDLQPL-NADIL--PEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGS 192
           +  + RSDL+P+ N D     EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTGS
Sbjct: 124 VEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGS 183

Query: 193 DINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGD 252
           D+NW+QC+PC DCYQQ+DP+F+PT+SS++ SL C +P C  LE S CRS KCLYQVSYGD
Sbjct: 184 DVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGD 243

Query: 253 GSFTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASS 312
           GSFTVG+  T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+S
Sbjct: 244 GSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATS 303

Query: 313 FSYCLVDRDSGSASTLEFNSA-APNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPP 372
           FSYCLVDRDSG +S+L+FNS         A LLR+ +++TFYYV L+G SVGG  + +P 
Sbjct: 304 FSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPD 363

Query: 373 SLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKK-ANSFALFDTCYDLS 432
           ++F +D SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  LKK ++S +LFDTCYD S
Sbjct: 364 AIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFS 423

Query: 433 SQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 492
           S S V +PTV+FHF+GGKSL L  KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGTR
Sbjct: 424 SLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTR 483

BLAST of CmaCh18G000350 vs. TAIR10
Match: AT1G25510.1 (AT1G25510.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 551.6 bits (1420), Expect = 4.9e-157
Identity = 278/489 (56.85%), Postives = 373/489 (76.28%), Query Frame = 1

Query: 14  FAIFLFLF-------LFLSLFPFTLSSSAS-LDVSTSLKQAHQVLKFDPEILTSFHQQEL 73
           ++ F F+F       +F  + P T +++ S L+V+ S+ +      F        +QQE 
Sbjct: 5   YSFFFFIFFLTSHSSVFSRILPETSTTTTSILNVADSIHRTKYTSSF------RLNQQE- 64

Query: 74  LLPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLT 133
                 S+SSSFSLQLH R ++R   H+DY+SL L+RL+RD++RVKSL  RL+  +++++
Sbjct: 65  --EQTHSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNIS 124

Query: 134 RSDLQPLNADILPE--DLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 193
           ++DL+P++     E  D+  P++SG++QGSGEYF+RVG+G+PA+  YMVLDTGSD+NWLQ
Sbjct: 125 KADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQ 184

Query: 194 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVG 253
           C PC DCY QT+PIF+P++SSS+  L C++P C ALE+S CR+A CLY+VSYGDGS+TVG
Sbjct: 185 CTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVG 244

Query: 254 QFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLV 313
            F TETL+ G++  + NVA+GCGH NEGLF+G+AGLLGLGGG L+L SQ+  +SFSYCLV
Sbjct: 245 DFATETLTIGST-LVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLV 304

Query: 314 DRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDD 373
           DRDS SAST++F ++   D+V A LLR+ +++TFYY+ LTG+SVGG  L IP S F++D+
Sbjct: 305 DRDSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDE 364

Query: 374 SGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIP 433
           SG GGII+DSGTA+TRLQT+ YNSLRD+FV+ T  L+KA   A+FDTCY+LS+++ V +P
Sbjct: 365 SGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVP 424

Query: 434 TVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 493
           TV+FHF GGK L L  KNY+IPVDS GTFC AFAPT SSL+IIGNVQQQGTRV FDLANS
Sbjct: 425 TVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANS 483

BLAST of CmaCh18G000350 vs. TAIR10
Match: AT1G01300.1 (AT1G01300.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 398.7 bits (1023), Expect = 5.3e-111
Identity = 240/488 (49.18%), Postives = 307/488 (62.91%), Query Frame = 1

Query: 16  IFLFLFLFLSLFPFT-LSSSASLDVSTSLKQAHQVLKFDPEILT-SFHQQELLLPANSSS 75
           +F   F FLSL  F+ L S  +L  ++        + F P+  + S  + E    ++S S
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSES 68

Query: 76  SSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND-RLEFGLSDLTRSDLQPL 135
           SSS +L L   DAL +    D   L  SRL RDS RVKS+     +    ++T +     
Sbjct: 69  SSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPR--- 128

Query: 136 NADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 195
                P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC  CY 
Sbjct: 129 -----PGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYS 188

Query: 196 QTDPIFDPTTSSSFTSLPCESPHCQALEMSGC--RSAKCLYQVSYGDGSFTVGQFVTETL 255
           Q+DPIFDP  S ++ ++PC SPHC+ L+ +GC  R   CLYQVSYGDGSFTVG F TETL
Sbjct: 189 QSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETL 248

Query: 256 SFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSL---TSQMKASSFSYCLVDRDS 315
           +F     +  VALGCGHDNEGLF+G+AGLLGLG G LS    T       FSYCLVDR +
Sbjct: 249 TF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSA 308

Query: 316 GS--ASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPL-SIPPSLFQLDDS 375
            S  +S +  N+A    +    LL + +++TFYYV L G+SVGG  +  +  SLF+LD  
Sbjct: 309 SSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQI 368

Query: 376 GHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPT 435
           G+GG+I+DSGT++TRL   AY ++RDAF      LK+A  F+LFDTC+DLS+ + V +PT
Sbjct: 369 GNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPT 428

Query: 436 VSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSV 493
           V  HF G   + L   NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +DLA+S 
Sbjct: 429 VVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSR 484

BLAST of CmaCh18G000350 vs. TAIR10
Match: AT3G61820.1 (AT3G61820.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 384.0 bits (985), Expect = 1.4e-106
Identity = 243/503 (48.31%), Postives = 304/503 (60.44%), Query Frame = 1

Query: 7   NQLPFCPFAIFLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELL 66
           N L F  FA+  F            +SSAS    T +     V         S+ + E L
Sbjct: 7   NTLAFSVFAVLFF------------TSSASSQYQTLV-----VNTLPSSATLSWPESESL 66

Query: 67  LPAN-SSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLND--RLEFGLSD 126
              + S S++S S+ L   DAL +        L   RL RDS RVKS+     +  G + 
Sbjct: 67  TDESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNA 126

Query: 127 LTRSDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 186
             R+   P  A       S  ++SG SQGSGEYF R+GVG PA   YMVLDTGSD+ WLQ
Sbjct: 127 TKRT---PRTAG----GFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQ 186

Query: 187 CQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQAL-EMSGC---RSAKCLYQVSYGDGS 246
           C PC  CY QTD IFDP  S +F ++PC S  C+ L + S C   RS  CLYQVSYGDGS
Sbjct: 187 CSPCKACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGS 246

Query: 247 FTVGQFVTETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMK---AS 306
           FT G F TETL+F +   +++V LGCGHDNEGLF+G+AGLLGLG G LS  SQ K     
Sbjct: 247 FTEGDFSTETLTF-HGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNG 306

Query: 307 SFSYCLVDR-DSGSA----STLEF-NSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGH 366
            FSYCLVDR  SGS+    ST+ F N+A P  SV   LL + +++TFYY+ L G+SVGG 
Sbjct: 307 KFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGS 366

Query: 367 PL-SIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFD 426
            +  +  S F+LD +G+GG+I+DSGT++TRL   AY +LRDAF      LK+A S++LFD
Sbjct: 367 RVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFD 426

Query: 427 TCYDLSSQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNV 486
           TC+DLS  + V +PTV FHF GG+ + L   NYLIPV++ G FCFAFA T  SLSIIGN+
Sbjct: 427 TCFDLSGMTTVKVPTVVFHFGGGE-VSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNI 483

Query: 487 QQQGTRVNFDLANSVVAFSPNKC 493
           QQQG RV +DL  S V F    C
Sbjct: 487 QQQGFRVAYDLVGSRVGFLSRAC 483

BLAST of CmaCh18G000350 vs. TAIR10
Match: AT3G20015.1 (AT3G20015.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 377.1 bits (967), Expect = 1.7e-104
Identity = 206/480 (42.92%), Postives = 288/480 (60.00%), Query Frame = 1

Query: 17  FLFLFLFLSLFPFTLSSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPANSSSSSS 76
           F FL L L L   +  S     +   L+    V    P+   +    E        SSS 
Sbjct: 7   FFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDE--------SSSK 66

Query: 77  FSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQPLNADI 136
           ++L+L  RD   +  + ++   + +R+ RD+ RV ++  R+   +   + S  +      
Sbjct: 67  YTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEV----- 126

Query: 137 LPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDP 196
              D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  CY+Q+DP
Sbjct: 127 --NDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDP 186

Query: 197 IFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSG 256
           +FDP  S S+T + C S  C  +E SGC S  C Y+V YGDGS+T G    ETL+F  + 
Sbjct: 187 VFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKT- 246

Query: 257 AINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASS---FSYCLVDRDSGSASTL 316
            + NVA+GCGH N G+FIG+AGLLG+GGGS+S   Q+   +   F YCLV R + S  +L
Sbjct: 247 VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSL 306

Query: 317 EFN-SAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVD 376
            F   A P  +    L+R+ R  +FYYV L GL VGG  + +P  +F L ++G GG+++D
Sbjct: 307 VFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMD 366

Query: 377 SGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSGG 436
           +GTA+TRL T AY + RD F   T  L +A+  ++FDTCYDLS    V +PTVSF+F+ G
Sbjct: 367 TGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEG 426

Query: 437 KSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
             L L  +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  V F PN C
Sbjct: 427 PVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of CmaCh18G000350 vs. NCBI nr
Match: gi|449439383|ref|XP_004137465.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus])

HSP 1 Score: 786.2 bits (2029), Expect = 3.4e-224
Identity = 402/486 (82.72%), Postives = 436/486 (89.71%), Query Frame = 1

Query: 16  IFLFLFLFLSLFPFTLS---------SSASLDVSTSLKQAHQVLKFDPEILTSFHQQELL 75
           +FLFLF FLSLFPFTLS         SSASLDVS SL+QA+QVLKFDP    SF QQ  L
Sbjct: 9   LFLFLF-FLSLFPFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQQVHL 68

Query: 76  LPANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTR 135
           +P+NSS   SFSLQLHPRD+L NAGH DY+SLVLSRL RDSSRVKS+ DRLEF LS+L R
Sbjct: 69  VPSNSSF--SFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSELKR 128

Query: 136 SDLQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQP 195
           SDL+PL  +ILPEDLSTPI+SG+SQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQP
Sbjct: 129 SDLEPLKTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQP 188

Query: 196 CTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFV 255
           CTDCYQQTDPIFDP +SSSF SLPCES  CQALE SGCR++KCLYQVSYGDGSFTVG+FV
Sbjct: 189 CTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGEFV 248

Query: 256 TETLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRD 315
            ETL+FGNSG INNVA+GCGHDNEGLF+GSAGLLGLGGGSLSLTSQMKASSFSYCLVDRD
Sbjct: 249 IETLTFGNSGMINNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRD 308

Query: 316 SGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGH 375
           S S+S LEFNSAAP+DSVNA LL+SG+V+TFYYV LTG+SVGG  LSIPP+LFQ+DDSG+
Sbjct: 309 SSSSSDLEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDSGY 368

Query: 376 GGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVS 435
           GGIIVDSGTAITRLQTQAYN+LRDAFV  TPYLKK N FALFDTCYDLSSQSRVTIPTVS
Sbjct: 369 GGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPTVS 428

Query: 436 FHFSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVA 493
           F F+GGKSLQL PKNYLIPVDS GTFCFAFAPTTSSLSIIGNVQQQGTRV++DLANSVV 
Sbjct: 429 FEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSVVG 488

BLAST of CmaCh18G000350 vs. NCBI nr
Match: gi|659066703|ref|XP_008457107.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo])

HSP 1 Score: 781.9 bits (2018), Expect = 6.3e-223
Identity = 401/484 (82.85%), Postives = 431/484 (89.05%), Query Frame = 1

Query: 18  LFLFLFLSLFPFTLS---------SSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLP 77
           LFLF F SLFPF LS         SSASLDVS SL+QA+QVLKFDP    SF QQ  L+P
Sbjct: 9   LFLFFF-SLFPFALSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQQVRLVP 68

Query: 78  ANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSD 137
           +NSS   SFSLQLHPRD+L NAGH DY+SLVLSRL RDSSRVKS+ DRLEF LS+L RSD
Sbjct: 69  SNSSF--SFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSELKRSD 128

Query: 138 LQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCT 197
           L+PL  +ILPEDLSTPIVSG+SQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCT
Sbjct: 129 LEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCT 188

Query: 198 DCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTE 257
           DCYQQTDPIFDP +SSSF SLPCES  CQALE SGCR++KCLYQVSYGDGSFTVG+FVTE
Sbjct: 189 DCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGEFVTE 248

Query: 258 TLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSG 317
           TL+FGNSG I NVA+GCGHDNEGLF+GSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 
Sbjct: 249 TLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSS 308

Query: 318 SASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGG 377
           S+S LEFNSAAP DSVNA LL+SGRV+TFYYV LTG+SVGG  LSIPP+LFQ+DDSG+GG
Sbjct: 309 SSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDSGYGG 368

Query: 378 IIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFH 437
           IIVDSGTAITRLQTQAYN+LRDAFV  TPYLKK N FALFDTCYDLSSQSRVTIPTVSF 
Sbjct: 369 IIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPTVSFE 428

Query: 438 FSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFS 493
           F+GGKSLQL PKNYLIPVDS GTFCFAFAPTTSSLSIIGNVQQQGTRV++DLANSVV FS
Sbjct: 429 FAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSVVGFS 488

BLAST of CmaCh18G000350 vs. NCBI nr
Match: gi|657966098|ref|XP_008374731.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1 [Malus domestica])

HSP 1 Score: 670.2 bits (1728), Expect = 2.7e-189
Identity = 335/484 (69.21%), Postives = 395/484 (81.61%), Query Frame = 1

Query: 17  FLFLFLFLSLFPFTLSS--------SASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLP 76
           F +L  F + F  T  S        +  LDVS SL+QAH VL FDPE L     Q     
Sbjct: 6   FFYLAFFSAFFTLTSLSRTLPESETATFLDVSASLRQAHAVLSFDPEFLKPLESQSQ--E 65

Query: 77  ANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSD 136
           +   +SS+FSLQLHPRDAL N+ H DY+SL L+RLDRDS+RV SL+ +L+  L  + +SD
Sbjct: 66  SRPLNSSAFSLQLHPRDALFNSQHKDYKSLTLTRLDRDSARVNSLHTKLQLALQGIKKSD 125

Query: 137 LQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCT 196
           L+P++A+ILP+DLSTP+VSG SQGSGEYFSR+GVG PAK FYMVLDTGSD+NWLQC PC+
Sbjct: 126 LEPMHAEILPQDLSTPVVSGVSQGSGEYFSRIGVGTPAKSFYMVLDTGSDVNWLQCNPCS 185

Query: 197 DCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTE 256
           DCYQQ+DP+F P  SS+++ L C++P CQAL +S CR+ KCLYQVSYGDGS+TVG +VTE
Sbjct: 186 DCYQQSDPVFSPAGSSTYSQLTCDAPQCQALHVSACRANKCLYQVSYGDGSYTVGNYVTE 245

Query: 257 TLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSG 316
           TLSFGNSG+IN +ALGCGHDNEGLF+G+AGLLGLGGG LSLTSQ KASSFSYCLV+RDS 
Sbjct: 246 TLSFGNSGSINKIALGCGHDNEGLFVGAAGLLGLGGGPLSLTSQFKASSFSYCLVNRDSS 305

Query: 317 SASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGG 376
           ++STLEFNSA P DSV A L+++G+V+TFYYV LTG SVGG P+S+PPSLF +D SG+GG
Sbjct: 306 ASSTLEFNSAPPADSVTAPLVQNGKVDTFYYVGLTGFSVGGQPVSVPPSLFAVDQSGNGG 365

Query: 377 IIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFH 436
           IIVDSGTAITRLQTQAYNSLRDAF R T  L  A+SFALFDTCYDLSS++RV +PTV+F 
Sbjct: 366 IIVDSGTAITRLQTQAYNSLRDAFKRFTSDLPTASSFALFDTCYDLSSRTRVQVPTVAFQ 425

Query: 437 FSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFS 493
           FSGGKSL L  KNYLIPVDSAGTFCFAFAPT+SSLSIIGNVQQQG RV++DLAN  V FS
Sbjct: 426 FSGGKSLSLPAKNYLIPVDSAGTFCFAFAPTSSSLSIIGNVQQQGIRVSYDLANKRVGFS 485

BLAST of CmaCh18G000350 vs. NCBI nr
Match: gi|702494967|ref|XP_010036867.1| (PREDICTED: LOW QUALITY PROTEIN: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Eucalyptus grandis])

HSP 1 Score: 666.8 bits (1719), Expect = 3.0e-188
Identity = 333/464 (71.77%), Postives = 391/464 (84.27%), Query Frame = 1

Query: 32  SSSASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLPANS--SSSSSFSLQLHPRDALRN 91
           S +A+LDVS SL+    VL FDP+  TSF +QE         +SS SFS++LHPR++L  
Sbjct: 33  SPTATLDVSGSLQNTRGVLSFDPQSQTSFSEQEQEQEQRQKQTSSGSFSVRLHPRESLVR 92

Query: 92  AGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSDLQPLNADILPEDLSTPIVSGS 151
             + +Y++LVL+RL RDS+RV +L  +L   L+++TR+DL PL  +I PEDLSTP+VSG+
Sbjct: 93  TPYREYKALVLARLGRDSARVDALASKLNLALNNVTRTDLTPLRTEIQPEDLSTPVVSGT 152

Query: 152 SQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSL 211
           SQGSGEYFSR+GVG PAK FYMVLDTGSD+NWLQCQPCTDCYQQTDP+F P++SSS+T+L
Sbjct: 153 SQGSGEYFSRIGVGTPAKQFYMVLDTGSDVNWLQCQPCTDCYQQTDPVFTPSSSSSYTAL 212

Query: 212 PCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTETLSFGNSGAINNVALGCGHDN 271
            CESP C +LE+S CR+ KCLYQVSYGDGSFTVG FVTET+SFG+SGA+NNVALGCGHDN
Sbjct: 213 TCESPQCGSLEVSACRTGKCLYQVSYGDGSFTVGDFVTETVSFGDSGAVNNVALGCGHDN 272

Query: 272 EGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSASTLEFNSAAPN-DSVNAQL 331
           EGLF+GSAGLLGLGGG LSLT Q+KASSFSYCLVDRDS  +STL+FNSAAP  +SV A L
Sbjct: 273 EGLFMGSAGLLGLGGGPLSLTKQVKASSFSYCLVDRDSSKSSTLDFNSAAPGAESVFAPL 332

Query: 332 LRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSL 391
           +++G+V+TFYYV LTG+SVGG  +SIPPSLFQ+D+SG GGIIVDSGTAITRLQTQAYNSL
Sbjct: 333 VKNGKVDTFYYVGLTGMSVGGKTVSIPPSLFQMDESGDGGIIVDSGTAITRLQTQAYNSL 392

Query: 392 RDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFHFSGGKSLQLAPKNYLIPVDS 451
           RDAFV+LTP LK  +  ALFDTCYD SS S V +PTVSF F+GG+SL L  KNYLIPVDS
Sbjct: 393 RDAFVKLTPDLKSTSGVALFDTCYDFSSLSSVQVPTVSFQFTGGRSLALPAKNYLIPVDS 452

Query: 452 AGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFSPNKC 493
           AGTFCFAFAPTTSSLSIIGNVQQQGTRV FDLANS V F+PNKC
Sbjct: 453 AGTFCFAFAPTTSSLSIIGNVQQQGTRVTFDLANSRVGFTPNKC 496

BLAST of CmaCh18G000350 vs. NCBI nr
Match: gi|694319400|ref|XP_009346910.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Pyrus x bretschneideri])

HSP 1 Score: 665.6 bits (1716), Expect = 6.6e-188
Identity = 336/484 (69.42%), Postives = 394/484 (81.40%), Query Frame = 1

Query: 17  FLFLFLFLSLFPFTLSS--------SASLDVSTSLKQAHQVLKFDPEILTSFHQQELLLP 76
           F +L  F + F  T  S        +  LDVS SL+QAH VL FDPE L     Q     
Sbjct: 6   FFYLAFFSAFFTLTSLSRTLPESETATFLDVSASLRQAHAVLSFDPESLKPLESQSQ--E 65

Query: 77  ANSSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSRVKSLNDRLEFGLSDLTRSD 136
           +   +SS+ SLQLHPRDAL N+ H DY+SL  +RL RDS+RV SL+ +L+  L  + +SD
Sbjct: 66  SRPLNSSALSLQLHPRDALFNSQHKDYKSLTRTRLYRDSARVHSLHSKLQLALQGIKKSD 125

Query: 137 LQPLNADILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCT 196
           L+P++A ILP+DLSTP+VSG SQGSGEYFSR+GVG PAK FYMV DTGSD+NWLQC PC+
Sbjct: 126 LEPMHAKILPQDLSTPVVSGVSQGSGEYFSRIGVGTPAKSFYMVPDTGSDVNWLQCDPCS 185

Query: 197 DCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRSAKCLYQVSYGDGSFTVGQFVTE 256
           DCYQQ+DP+F+P  SSS++ L C++P CQALE+S CR+ KCLYQVSYGDGS+TVG +VTE
Sbjct: 186 DCYQQSDPVFNPAGSSSYSQLTCDAPQCQALEVSACRANKCLYQVSYGDGSYTVGDYVTE 245

Query: 257 TLSFGNSGAINNVALGCGHDNEGLFIGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSG 316
           TLSFGNSG+IN +ALGCGHDNEGLF+G+AGLLGLGGG LSLTSQ KASSFSYCLV+RDS 
Sbjct: 246 TLSFGNSGSINKIALGCGHDNEGLFVGAAGLLGLGGGPLSLTSQFKASSFSYCLVNRDSS 305

Query: 317 SASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGGHPLSIPPSLFQLDDSGHGG 376
           ++STLEFNSA P DSV A LL+SG+V+TFYYV LTG SVGG P+S+PPSLF +D+SG+GG
Sbjct: 306 ASSTLEFNSAPPADSVTAPLLQSGKVSTFYYVGLTGFSVGGQPVSVPPSLFAVDESGNGG 365

Query: 377 IIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFDTCYDLSSQSRVTIPTVSFH 436
           IIVDSGTAITRLQTQAYNSLRDAF R T  L  A+SFALFDTCYDLSS++RV +PTV+F 
Sbjct: 366 IIVDSGTAITRLQTQAYNSLRDAFKRYTRDLPTASSFALFDTCYDLSSRTRVQVPTVAFQ 425

Query: 437 FSGGKSLQLAPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVAFS 493
           FSGGKSL L  KNYLIPVDSAGTFCFAFAPT+SSLSIIGNVQQQGTRV++DLAN  V FS
Sbjct: 426 FSGGKSLSLPAKNYLIPVDSAGTFCFAFAPTSSSLSIIGNVQQQGTRVSYDLANKRVGFS 485

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ASPG1_ARATH2.5e-16359.96Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 ... [more]
APF2_ARATH9.5e-11049.18Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1[more]
ASPG2_ARATH2.9e-10342.92Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 ... [more]
NEP2_NEPGR1.7e-7443.58Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1[more]
NEP1_NEPGR6.4e-7444.96Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LQD2_CUCSA2.3e-22482.72Uncharacterized protein OS=Cucumis sativus GN=Csa_1G042220 PE=3 SV=1[more]
M5WPB5_PRUPE1.2e-18367.97Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004726mg PE=3 SV=1[more]
A0A0R0IA82_SOYBN1.3e-18268.62Uncharacterized protein OS=Glycine max GN=GLYMA_09G187200 PE=3 SV=1[more]
A0A061FUN2_THECC7.1e-18167.22Eukaryotic aspartyl protease family protein OS=Theobroma cacao GN=TCM_012281 PE=... [more]
V7C608_PHAVU6.0e-18067.56Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_004G160200g PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G18490.11.4e-16459.96 Eukaryotic aspartyl protease family protein[more]
AT1G25510.14.9e-15756.85 Eukaryotic aspartyl protease family protein[more]
AT1G01300.15.3e-11149.18 Eukaryotic aspartyl protease family protein[more]
AT3G61820.11.4e-10648.31 Eukaryotic aspartyl protease family protein[more]
AT3G20015.11.7e-10442.92 Eukaryotic aspartyl protease family protein[more]
Match NameE-valueIdentityDescription
gi|449439383|ref|XP_004137465.1|3.4e-22482.72PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus][more]
gi|659066703|ref|XP_008457107.1|6.3e-22382.85PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucumis melo][more]
gi|657966098|ref|XP_008374731.1|2.7e-18969.21PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1 [Malus domestica][more]
gi|702494967|ref|XP_010036867.1|3.0e-18871.77PREDICTED: LOW QUALITY PROTEIN: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [... [more]
gi|694319400|ref|XP_009346910.1|6.6e-18869.42PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Pyrus x bretschneider... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001461Aspartic_peptidase_A1
IPR001969Aspartic_peptidase_AS
IPR021109Peptidase_aspartic_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009737 response to abscisic acid
biological_process GO:0009414 response to water deprivation
biological_process GO:0009627 systemic acquired resistance
biological_process GO:0050896 response to stimulus
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005575 cellular_component
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G000350.1CmaCh18G000350.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 67..492
score: 1.9E-247coord: 13..35
score: 1.9E
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 171..182
score: -coord: 369..380
scor
IPR021109Aspartic peptidase domainGENE3DG3DSA:2.40.70.10coord: 327..492
score: 5.6E-49coord: 141..309
score: 3.4
IPR021109Aspartic peptidase domainunknownSSF50630Acid proteasescoord: 150..492
score: 1.29
NoneNo IPR availablePANTHERPTHR13683:SF274ASPARTYL PROTEASE FAMILY PROTEINcoord: 67..492
score: 1.9E-247coord: 13..35
score: 1.9E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh18G000350Cucsa.113690Cucumber (Gy14) v1cgycmaB0268
CmaCh18G000350Cucsa.198960Cucumber (Gy14) v1cgycmaB0587
CmaCh18G000350Cla018984Watermelon (97103) v1cmawmB404
CmaCh18G000350Cla018513Watermelon (97103) v1cmawmB396
CmaCh18G000350Csa1G042220Cucumber (Chinese Long) v2cmacuB390
CmaCh18G000350Csa1G021980Cucumber (Chinese Long) v2cmacuB393
CmaCh18G000350MELO3C002079Melon (DHL92) v3.5.1cmameB365
CmaCh18G000350MELO3C017297Melon (DHL92) v3.5.1cmameB372
CmaCh18G000350ClCG06G014930Watermelon (Charleston Gray)cmawcgB371
CmaCh18G000350ClCG04G011230Watermelon (Charleston Gray)cmawcgB363
CmaCh18G000350CSPI01G06670Wild cucumber (PI 183967)cmacpiB394
CmaCh18G000350CSPI01G03680Wild cucumber (PI 183967)cmacpiB397
CmaCh18G000350CmoCh13G010750Cucurbita moschata (Rifu)cmacmoB399
CmaCh18G000350Lsi06G013410Bottle gourd (USVL1VR-Ls)cmalsiB391
CmaCh18G000350Lsi02G001680Bottle gourd (USVL1VR-Ls)cmalsiB370
CmaCh18G000350Cp4.1LG20g01100Cucurbita pepo (Zucchini)cmacpeB439
CmaCh18G000350Cp4.1LG04g14080Cucurbita pepo (Zucchini)cmacpeB452
CmaCh18G000350MELO3C002079.2Melon (DHL92) v3.6.1cmamedB424
CmaCh18G000350MELO3C017297.2Melon (DHL92) v3.6.1cmamedB429
CmaCh18G000350CsaV3_1G003720Cucumber (Chinese Long) v3cmacucB0466
CmaCh18G000350CsaV3_1G006520Cucumber (Chinese Long) v3cmacucB0464
CmaCh18G000350Cla97C04G078100Watermelon (97103) v2cmawmbB422
CmaCh18G000350Cla97C06G124420Watermelon (97103) v2cmawmbB432
CmaCh18G000350Bhi08G001011Wax gourdcmawgoB0520
CmaCh18G000350CsGy1G003610Cucumber (Gy14) v2cgybcmaB088
CmaCh18G000350CsGy1G006370Cucumber (Gy14) v2cgybcmaB085
CmaCh18G000350Carg09148Silver-seed gourdcarcmaB0834
CmaCh18G000350Carg22757Silver-seed gourdcarcmaB0722
CmaCh18G000350Carg04805Silver-seed gourdcarcmaB0541
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh18G000350CmaCh11G002640Cucurbita maxima (Rimu)cmacmaB139
CmaCh18G000350CmaCh13G010350Cucurbita maxima (Rimu)cmacmaB217
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh18G000350Cucurbita pepo (Zucchini)cmacpeB416
CmaCh18G000350Cucurbita maxima (Rimu)cmacmaB100
CmaCh18G000350Cucurbita moschata (Rifu)cmacmoB389
CmaCh18G000350Cucurbita moschata (Rifu)cmacmoB408