CmaCh17G013420 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh17G013420
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cma_Chr17 : 9000410 .. 9001669 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTTGTGTTTGGTTCCAAAATAGTAGAGTTTGTTCATATTGCAGAATATGTCGATAAAAGAAGTTCCAGTGATAGACCTTGGTTGTCAGAGCTTGACTGAATCGACTTCATCTTCTCTACTTAGAGCATGCAACAAATTGGGGTTCTTCTTCATCAGAAACCATGGCGTTTCAAAGGAGTTTTACGAGAAACTCATCTCTGGCGCTGATGAGCTTTTTTCTTCTCCCGAGGAGACCAAGCAACAACTGAAAATGGGAGTTTCCTCGTACACCCCGAGATTTTTGGTGTCGCCTTTCTTTGAGAGCTTCAGGGTTTCGGGACCTAACTTCTCAGCCTCTGCTGCTGCGCAAGGTTTCACTCAATCCCTATTCGGCCACCATGCTACTGAATTCAGGTTGTCTTCTACTTAGAAATGTTTGCTTTCATCGCTATTAAAATCTGCCCAATGAGCTTTATTTTATATGATTGTTCTATCATTATCTACTTTATTTAGACACTAATTTGTGTTTGTATGTTCGAGTTTATTTGCAGCAATCTTCTAGACGAATATGGAAGAAAAATGAAAGAAGTATCAAAAAGAGTAGTGAAGCTATTACTAAACGTCTTGGGAGATAATATTGAAAATAAATTCTATGATCCCGAGTTTAGCAACTGCAATGGCTACCTAAGAATAAACGGCTTCACTCCTCTAGACATGAATGCCGAAGAAGAAATTGAAGTGTTCAAGAAACACACCGACCTCAGCTACGTAACGATAGTGTTCCAAAATGACATCAGGGGGCTACAAATGAGATCGAAAGGAGGCGACTGGATCGACGTAAGGCTGAGCGAGGATGGTATTCTTGTGGTGAACATTGGAGATTATATGGAGGCTTGGAGCAACGGGAGGCTGAGATCTGCCGTACATAGAGTTGTTTTAAGGAAAAATATTATGAAGAGATTCTCTATCGCTTTCTTATCAACACCGGAAGATGAATCTAGAGAAATATATGCACCGAGTGAAGTCGTTGGAGAAGGGAAATCAAGACTTTACCGCTCGTTTTCAGTCCAACAATATAAGCATTTTAGGGAAAACAATTACGAGAAAGTTGGAGCCACGATTAAGGATTTTGTTGGAATATGATGTCGTTGGATTATTTCTTCCGATTCGACTACAACAAAAAAATATTAAAAATATTAAATTATCGATATTTGGGTCGTAAATAATTATCTTACGATGTACTAGAATATACACTGGAATTGATATGTGATGACATCGG

mRNA sequence

CATTTGTGTTTGGTTCCAAAATAGTAGAGTTTGTTCATATTGCAGAATATGTCGATAAAAGAAGTTCCAGTGATAGACCTTGGTTGTCAGAGCTTGACTGAATCGACTTCATCTTCTCTACTTAGAGCATGCAACAAATTGGGGTTCTTCTTCATCAGAAACCATGGCGTTTCAAAGGAGTTTTACGAGAAACTCATCTCTGGCGCTGATGAGCTTTTTTCTTCTCCCGAGGAGACCAAGCAACAACTGAAAATGGGAGTTTCCTCGTACACCCCGAGATTTTTGGTGTCGCCTTTCTTTGAGAGCTTCAGGGTTTCGGGACCTAACTTCTCAGCCTCTGCTGCTGCGCAAGGTTTCACTCAATCCCTATTCGGCCACCATGCTACTGAATTCAGCAATCTTCTAGACGAATATGGAAGAAAAATGAAAGAAGTATCAAAAAGAGTAGTGAAGCTATTACTAAACGTCTTGGGAGATAATATTGAAAATAAATTCTATGATCCCGAGTTTAGCAACTGCAATGGCTACCTAAGAATAAACGGCTTCACTCCTCTAGACATGAATGCCGAAGAAGAAATTGAAGTGTTCAAGAAACACACCGACCTCAGCTACGTAACGATAGTGTTCCAAAATGACATCAGGGGGCTACAAATGAGATCGAAAGGAGGCGACTGGATCGACGTAAGGCTGAGCGAGGATGGTATTCTTGTGGTGAACATTGGAGATTATATGGAGGCTTGGAGCAACGGGAGGCTGAGATCTGCCGTACATAGAGTTGTTTTAAGGAAAAATATTATGAAGAGATTCTCTATCGCTTTCTTATCAACACCGGAAGATGAATCTAGAGAAATATATGCACCGAGTGAAGTCGTTGGAGAAGGGAAATCAAGACTTTACCGCTCGTTTTCAGTCCAACAATATAAGCATTTTAGGGAAAACAATTACGAGAAAGTTGGAGCCACGATTAAGGATTTTGTTGGAATATGATGTCGTTGGATTATTTCTTCCGATTCGACTACAACAAAAAAATATTAAAAATATTAAATTATCGATATTTGGGTCGTAAATAATTATCTTACGATGTACTAGAATATACACTGGAATTGATATGTGATGACATCGG

Coding sequence (CDS)

ATGTCGATAAAAGAAGTTCCAGTGATAGACCTTGGTTGTCAGAGCTTGACTGAATCGACTTCATCTTCTCTACTTAGAGCATGCAACAAATTGGGGTTCTTCTTCATCAGAAACCATGGCGTTTCAAAGGAGTTTTACGAGAAACTCATCTCTGGCGCTGATGAGCTTTTTTCTTCTCCCGAGGAGACCAAGCAACAACTGAAAATGGGAGTTTCCTCGTACACCCCGAGATTTTTGGTGTCGCCTTTCTTTGAGAGCTTCAGGGTTTCGGGACCTAACTTCTCAGCCTCTGCTGCTGCGCAAGGTTTCACTCAATCCCTATTCGGCCACCATGCTACTGAATTCAGCAATCTTCTAGACGAATATGGAAGAAAAATGAAAGAAGTATCAAAAAGAGTAGTGAAGCTATTACTAAACGTCTTGGGAGATAATATTGAAAATAAATTCTATGATCCCGAGTTTAGCAACTGCAATGGCTACCTAAGAATAAACGGCTTCACTCCTCTAGACATGAATGCCGAAGAAGAAATTGAAGTGTTCAAGAAACACACCGACCTCAGCTACGTAACGATAGTGTTCCAAAATGACATCAGGGGGCTACAAATGAGATCGAAAGGAGGCGACTGGATCGACGTAAGGCTGAGCGAGGATGGTATTCTTGTGGTGAACATTGGAGATTATATGGAGGCTTGGAGCAACGGGAGGCTGAGATCTGCCGTACATAGAGTTGTTTTAAGGAAAAATATTATGAAGAGATTCTCTATCGCTTTCTTATCAACACCGGAAGATGAATCTAGAGAAATATATGCACCGAGTGAAGTCGTTGGAGAAGGGAAATCAAGACTTTACCGCTCGTTTTCAGTCCAACAATATAAGCATTTTAGGGAAAACAATTACGAGAAAGTTGGAGCCACGATTAAGGATTTTGTTGGAATATGA

Protein sequence

MSIKEVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQQLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLDEYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVFKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENNYEKVGATIKDFVGI

Homology

[Close]

BLAST of CmaCh17G013420 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.3e-24
Identity = 92/312 (29.49%), Postives = 149/312 (47.76%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTS-----SSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSS 64
           ++PVIDL   +  E          ++ A  + GFF I NHG+ K+ +E ++    +LF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 65  PEETKQQLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLL 124
           P   K + +    S            S+R   P  SA++ AQ      F    +E S + 
Sbjct: 99  PFSVKVRERFSDLSKN----------SYRWGNP--SATSPAQYSVSEAFHIILSEVSRIS 158

Query: 125 DEYGRKMKEVSK------RVVKLLLNVLGD--NIENKFYDPEFSNCNGYLRINGFTPLDM 184
           D+       V        RV +++  +LG   N+ +++++  F   N +LR+N + P   
Sbjct: 159 DDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVF 218

Query: 185 NAEEEIEVFKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAW 244
            +E  +     HTD S++TI+ Q+ I GL++ + G  WI V+   +  L VNIGD  +A 
Sbjct: 219 GSE--VFGLVPHTDTSFLTILSQDQIGGLELENNG-QWISVKPCLEA-LTVNIGDMFQAL 278

Query: 245 SNGRLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQY 304
           SNG  +S  HRV+   NI +R SIAF   P       Y  +E+   G  + YR FS ++Y
Sbjct: 279 SNGVYQSVRHRVISPANI-ERMSIAFFVCP-------YLETEIDCFGYPKKYRRFSFREY 326

[Close]

BLAST of CmaCh17G013420 vs. Swiss-Prot
Match: G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.3e-23
Identity = 89/312 (28.53%), Postives = 153/312 (49.04%), Query Frame = 1


Query: 2   SIKEVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPE 61
           S   VPVIDL       + S  +  AC   G + + NHG+     E +      LFS P 
Sbjct: 52  STTTVPVIDLN----DPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPS 111

Query: 62  ETKQQLKM---GVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNL 121
             K +      GVS Y    + S F +     G  F+   +     + L+    T F ++
Sbjct: 112 HQKHKATRSPDGVSGYGIARISSFFPKLMWYEG--FTIVGSPLDHFRELWPQDYTRFCDI 171

Query: 122 LDEYGRKMKEVSKRVVKLLLNVLG---DNIENKFYDPEFSNCNGYLRINGFTPLDMNAEE 181
           + +Y   MK+++  ++ L+L+ LG   ++I+      +F      L++N + P   + + 
Sbjct: 172 VVQYDETMKKLAGTLMCLMLDSLGITKEDIKWAGSKAQFEKACAALQLNSY-PSCPDPDH 231

Query: 182 EIEVFKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGR 241
            + +   HTD +++TI+ QNDI GLQ+  +G  WI V   + G LVVN+GD     SNG 
Sbjct: 232 AMGL-APHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQGG-LVVNVGDLFHILSNGL 291

Query: 242 LRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFR 301
             S +HRV++ +   +RFS+A+L  P   + EI   ++++G  K  LYRS +  +Y   +
Sbjct: 292 YPSVLHRVLVNRT-RQRFSVAYLYGP-PSNVEICPHAKLIGPTKPPLYRSVTWNEYLGTK 351

Query: 302 ENNYEKVGATIK 308
             ++ K  ++++
Sbjct: 352 AKHFNKALSSVR 352

[Close]

BLAST of CmaCh17G013420 vs. Swiss-Prot
Match: GAOX5_ARATH (Gibberellin 20 oxidase 5 OS=Arabidopsis thaliana GN=GA20OX5 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 7.3e-23
Identity = 90/321 (28.04%), Postives = 152/321 (47.35%), Query Frame = 1


Query: 5   EVPVIDL-----GCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSS 64
           ++P+IDL     G ++ T+  + ++ +AC   G F + NHG      EK +  +   F  
Sbjct: 62  DLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLVVNHGFKSGLAEKALEISSLFFGL 121

Query: 65  PEETK---QQLKMGVSSYTP----RFLVS-PFFESFRVS---GPNFSASAAAQGFTQSLF 124
            ++ K    ++   +S YT     RF  + P+ E+  ++   GP        + F  S  
Sbjct: 122 SKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLTLAFKKGPPH----VVEDFLTSRL 181

Query: 125 GHHATEFSNLLDEYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTP 184
           G+H  E   +  E+   M  +   +++LL   +G   +  +Y   F + +G  R N + P
Sbjct: 182 GNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLK-DRTYYRRFFEDGSGIFRCNYYPP 241

Query: 185 LDMNAEEEIEVFKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYM 244
                E+ + V   H D + +T++ Q+D+ GL++ +  G W  VR    G LVVN+GD  
Sbjct: 242 CKQ-PEKALGV-GPHNDPTAITVLLQDDVVGLEVFA-AGSWQTVR-PRPGALVVNVGDTF 301

Query: 245 EAWSNGRLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSV 304
            A SNG  RS  HR V+ K  ++R S+ F S P ++   +  P  V GE  SR Y  F+ 
Sbjct: 302 MALSNGNYRSCYHRAVVNKEKVRR-SLVFFSCPREDKIIVPPPELVEGEEASRKYPDFTW 361

Query: 305 QQYKHFRENNYEKVGATIKDF 310
            Q + F ++ Y     T+ +F
Sbjct: 362 AQLQKFTQSGYRVDNTTLHNF 372

[Close]

BLAST of CmaCh17G013420 vs. Swiss-Prot
Match: YQK1_SCHPO (UPF0676 protein C1494.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPCC1494.01 PE=3 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 1.2e-22
Identity = 82/275 (29.82%), Postives = 131/275 (47.64%), Query Frame = 1


Query: 1   MSIKEVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSP 60
           M   EVP IDL  ++ T      LL AC   GF  ++NHG+  +  ++    AD+ F  P
Sbjct: 1   MGSLEVPCIDLS-ENDTSIVVKELLDACKNWGFVSLKNHGIPLDEIDRTFKLADKFFDIP 60

Query: 61  EETKQQ--LKMGV--SSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFS 120
            E KQ+   K G   S YT  F      +    S  +   S    GF      +     +
Sbjct: 61  VEEKQKYLFKGGRLHSGYTGHF--GEKLDMEHQSRGDLKESYDLAGFPDPKLENLCPFIA 120

Query: 121 NLLDEYGRKMKEVSKRVVKLL-LNVLGDNIENKFYDPEFSNCNGYLRINGFT-PLDMNAE 180
             +DE+ +  +   K  ++LL    +G  I   F+    S+    LR+  ++ P  +   
Sbjct: 121 EHMDEFLQFQRHCYKLTLRLLDFFAIGFGIPPDFFSKSHSSEEDVLRLLKYSIPEGVERR 180

Query: 181 EEIEVFKKHTDLSYVTIVFQNDIRGLQMRS----KGGDWIDVRLSEDGILVVNIGDYMEA 240
           E+ E    H+D   +T++FQ D  GL++R     K  DWI V +  D +++VNI D ++ 
Sbjct: 181 EDDEDAGAHSDYGSITLLFQRDAAGLEIRPPNFVKDMDWIKVNVQPD-VVLVNIADMLQF 240

Query: 241 WSNGRLRSAVHRVVLRKNIMKRFSIAFLSTPEDES 266
           W++G+LRS VHRV +   +  R +IA+  TP+ E+
Sbjct: 241 WTSGKLRSTVHRVRIDPGVKTRQTIAYFVTPDPET 271

[Close]

BLAST of CmaCh17G013420 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 2.8e-22
Identity = 95/322 (29.50%), Postives = 152/322 (47.20%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSL------LRACNKLGFFFIRNHGVSKEFYEKLISGADELFS 64
           +VP+IDL  Q+L    SS+L        AC K GFF + NHG+S+E            F 
Sbjct: 60  DVPLIDL--QNLLSDPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYTSRFFD 119

Query: 65  SPEETKQQL--KMG-----VSSYTPRFLVS-PFFE--SFRVSGPNFSASAAAQGFTQSLF 124
            P   KQ++  K G      SS+T RF    P+ E  SFR    + S S + Q +     
Sbjct: 120 MPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCD-DMSRSKSVQDYFCDAL 179

Query: 125 GHHATEFSNLLDEYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTP 184
           GH    F  +  EY   M  +S ++++LL   LG  ++  ++   F   +  +R+N + P
Sbjct: 180 GHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLG--VKRDYFREFFEENDSIMRLNYYPP 239

Query: 185 LDMNAEEEIEVFK-KHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDY 244
                + ++ +    H D + +TI+ Q+ + GLQ+  +   W  +R +     VVNIGD 
Sbjct: 240 C---IKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVEN-QWRSIRPNPKAF-VVNIGDT 299

Query: 245 MEAWSNGRLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFS 304
             A SN R +S +HR V+     +R S+AF   P+ + R +  P E++    SR Y  F+
Sbjct: 300 FMALSNDRYKSCLHRAVVNSE-SERKSLAFFLCPK-KDRVVTPPRELLDSITSRRYPDFT 359

Query: 305 VQQYKHFRENNYEKVGATIKDF 310
              +  F + +Y     T++ F
Sbjct: 360 WSMFLEFTQKHYRADMNTLQAF 369

[Close]

BLAST of CmaCh17G013420 vs. TrEMBL
Match: A0A0A0KW97_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 5.5e-94
Identity = 178/314 (56.69%), Postives = 242/314 (77.07%), Query Frame = 1


Query: 4   KEVPVIDLG-CQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSS-PE 63
           +E+ V+D+  C+ L+ES+  SL +ACN+ G F+I+NHGVSKE Y+KL +  DEL ++ PE
Sbjct: 10  EELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPE 69

Query: 64  ETKQ-QLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 123
           ETK+ +LK+G S YTPRF +SP+ ESF+  GPNFS  A+  GFT+ +FG   T+F  LLD
Sbjct: 70  ETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLD 129

Query: 124 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVF 183
           EYG  M E+S+R++KLLL  +GDN+E+KFY+ EFSNCNGYLRIN + P   N+ EEIE F
Sbjct: 130 EYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAP--RNSNEEIEAF 189

Query: 184 KKHTDLSYVTIVFQNDIRGLQMRSKGGD-WIDVRLSEDGILVVNIGDYMEAWSNGRLRSA 243
            KHTD+S VTI+FQ++I G+QM+ K GD W+DVR  ED +L VNIGD+++AWSN RLRSA
Sbjct: 190 GKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALL-VNIGDFLQAWSNERLRSA 249

Query: 244 VHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENNY 303
            HRVVL++++ KRFS+AF    +D+ RE+YAPSEVVGEG +R+Y+ FS ++Y+ +RENNY
Sbjct: 250 EHRVVLKQDV-KRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 309

Query: 304 EK-VGATIKDFVGI 313
            K VG  +++F GI
Sbjct: 310 RKIVGVPLREFAGI 319

[Close]

BLAST of CmaCh17G013420 vs. TrEMBL
Match: A0A068UKJ5_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00027923001 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.0e-76
Identity = 161/317 (50.79%), Postives = 218/317 (68.77%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           ++P +D+  + L   + SSL  AC++ GFF I NHG+SKE Y+K++S + +LFS P ETK
Sbjct: 10  KLPSLDIS-KPLDSCSLSSLTAACSEWGFFQITNHGISKELYDKILSLSKDLFSLPSETK 69

Query: 65  QQLKMG----VSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 124
             LK+G    + +YTP F+ SPFFES RVSGP+F+ASA  Q   + L   + +EFS +L 
Sbjct: 70  --LKLGPFSSLKTYTPHFIASPFFESLRVSGPDFAASA--QDSAEVLSAPNISEFSEILH 129

Query: 125 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLD-MNAEEEIEV 184
           EYGRKM E+SK V+KL+L  LG   E K+Y+ EFSNC GYLRIN + P + +  EEE E 
Sbjct: 130 EYGRKMTEISKEVLKLVLMSLGAEFEMKYYNSEFSNCQGYLRINNYAPPESLEEEEEAEG 189

Query: 185 FKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSA 244
              HTD+S +TIV+Q+++ GLQ++S+ G W+D+ +  +G LVVNIGD ++AWSN + RS+
Sbjct: 190 LGMHTDMSCITIVYQDEVGGLQVKSREGKWMDI-VPCEGTLVVNIGDVLQAWSNDKFRSS 249

Query: 245 VHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENN- 304
            HRVVLRK ++ RFS+AF    EDE + I AP EVVG   SR+Y+ F    Y  FRENN 
Sbjct: 250 EHRVVLRK-LVNRFSLAFFWCFEDE-KIILAPDEVVGRENSRIYKPFVCSDYLKFRENNE 309

Query: 305 ---YEKVGATIKDFVGI 313
              +EKVG T+KDF GI
Sbjct: 310 KGKFEKVGFTVKDFAGI 318

[Close]

BLAST of CmaCh17G013420 vs. TrEMBL
Match: B9R9V9_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1500930 PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.1e-75
Identity = 163/313 (52.08%), Postives = 218/313 (69.65%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           ++P +D+  Q L++S+ SSL  AC + GFF I NHGVSK+ Y KL   AD LF+ P E+K
Sbjct: 9   QLPALDIS-QPLSQSSLSSLSSACKEWGFFRIINHGVSKDLYRKLYFLADHLFNLPCESK 68

Query: 65  QQLKMGVSS----YTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 124
             +K+G SS    YTP F+ SPFFES RVSGP+F ASA  Q  +Q LF H   EFS  + 
Sbjct: 69  --IKIGPSSSIKTYTPHFIASPFFESLRVSGPDFFASA--QTSSQVLFHHPNPEFSEAMS 128

Query: 125 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVF 184
           EYG KM E+SKR+VK +L  +GD+   KFY+ +F+ C+GY+RIN ++P +  + EE+E  
Sbjct: 129 EYGNKMSELSKRIVKAVLISMGDDFAEKFYESDFNKCHGYMRINKYSPAE--SAEEVEGL 188

Query: 185 KKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAV 244
             HTD+S +TIV Q+DI GLQ+RSK G W+D+   ED  LVVNIGD M AWSNG+LRS+ 
Sbjct: 189 GMHTDMSCMTIVCQDDIGGLQVRSKLGKWMDINPCED-TLVVNIGDLMHAWSNGKLRSSE 248

Query: 245 HRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENN-- 304
           HRVVLR+ +  RFS+AF    ED+ + I+AP+EVVGEG  R+Y+ F  + Y +FRE++  
Sbjct: 249 HRVVLRRQV-HRFSLAFFWCFEDD-KVIFAPNEVVGEGNLRMYKPFVCRDYLNFRESSEK 308

Query: 305 --YEKVGATIKDF 310
             ++KVG TIKDF
Sbjct: 309 GKFDKVGFTIKDF 311

[Close]

BLAST of CmaCh17G013420 vs. TrEMBL
Match: A0A061FZ75_THECC (2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao GN=TCM_014890 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-75
Identity = 163/315 (51.75%), Postives = 213/315 (67.62%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           E+PV DL  Q L+ S+ SSL  AC + GFF I NHGVSK+ Y KL S ++ LFS P+E+K
Sbjct: 9   ELPVFDLS-QPLSPSSLSSLSLACREWGFFRITNHGVSKDLYRKLYSLSNHLFSLPDESK 68

Query: 65  QQLKMGVSS----YTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 124
             LK+G +S    YTP F+ SPFFES RVSGP+F ASA  Q     LF    +EFS +L 
Sbjct: 69  --LKLGPTSSLKTYTPHFIASPFFESLRVSGPDFFASA--QSSVDVLFEQPNSEFSEVLK 128

Query: 125 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVF 184
           EYG  M ++SK +V+ +L  LG+ +E K ++ EF NC+GYLRIN +TP     EEE++  
Sbjct: 129 EYGSLMTKLSKEIVEAILKSLGEELEKKSFESEFGNCHGYLRINNYTPPKSMKEEEVDGL 188

Query: 185 KKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAV 244
             HTD+S VTIV+Q++I GLQ+RSK G W+D+   ED  LVVNIGD M AWSNG+L S+ 
Sbjct: 189 GMHTDMSCVTIVYQDEIGGLQVRSKEGKWMDINPCED-TLVVNIGDLMHAWSNGKLSSSE 248

Query: 245 HRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENN-- 304
           HRVVL K I+ RFS+AF    EDE + I+AP+EVVGE   R+Y+ F    Y  FR+++  
Sbjct: 249 HRVVL-KRIVNRFSLAFFWCFEDE-KVIFAPNEVVGEDNLRIYKPFVCADYLKFRQSSEK 308

Query: 305 --YEKVGATIKDFVG 312
             ++KVG T+KDF G
Sbjct: 309 GRFDKVGFTVKDFAG 315

[Close]

BLAST of CmaCh17G013420 vs. TrEMBL
Match: D7TVY9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03440 PE=3 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.4e-75
Identity = 156/316 (49.37%), Postives = 212/316 (67.09%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           ++PV+DL  Q +  S  +SL +AC + GFF + NHG+ ++ + K+   +  +FS P ++K
Sbjct: 9   QLPVLDLS-QPVHPSFLTSLSQACEEWGFFHVMNHGIPQDLFSKVCLLSKHIFSLPADSK 68

Query: 65  QQLKMGVSS----YTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 124
             LK+G SS    YTPRF+ SPFFES R++GP+F ASA +      LF    +EFS  L 
Sbjct: 69  --LKLGPSSSIKTYTPRFIASPFFESLRIAGPDFLASAKSS--VAELFDQQNSEFSETLQ 128

Query: 125 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVF 184
           EYG KM E+SKR+V+ +L  LGD  E KF + EF+NC GYLR+  ++P +   +EE+E  
Sbjct: 129 EYGSKMVELSKRIVEAVLMSLGDGYERKFNESEFNNCQGYLRLVNYSPPECVEDEEVEGL 188

Query: 185 KKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAV 244
             HTD+S +TIV+Q++I GLQMRSKGG+WID+   E G LVVNIGD M+ WSNGRLRS+ 
Sbjct: 189 GMHTDMSCITIVYQDEIGGLQMRSKGGEWIDITPCE-GTLVVNIGDLMQGWSNGRLRSSE 248

Query: 245 HRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENN-- 304
           HRVVL K  + R S+AF    EDE + + AP E+VGEG SR+Y+ F   +Y  FRE+N  
Sbjct: 249 HRVVL-KRFVNRLSLAFFWCFEDE-KVVLAPDEIVGEGNSRIYKPFVCLEYLKFRESNEE 308

Query: 305 --YEKVGATIKDFVGI 313
             YEKVG T+KDF  +
Sbjct: 309 GKYEKVGYTVKDFAAL 316

[Close]

BLAST of CmaCh17G013420 vs. TAIR10
Match: AT4G23340.1 (AT4G23340.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 288.5 bits (737), Expect = 4.9e-78
Identity = 159/316 (50.32%), Postives = 214/316 (67.72%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           ++PV+DL  Q +  S  SSL  AC + GFF++ NHG+SKE + K+ S + ++F +P E+K
Sbjct: 9   QLPVLDL-TQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKAPLESK 68

Query: 65  QQLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLF-GHHATEFSNLLDEYG 124
             LK+G  SYTPR++ SP+FES  VSGP+FS SA A      LF  HH  E    + EYG
Sbjct: 69  --LKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASA--DVLFQDHHKPELRETMQEYG 128

Query: 125 RKMKEVSKRVVKLLLNV-LGDNIENKFYDPEFSNCNGYLRINGFTPL-DMNAEEE-IEVF 184
            KM E+SKR++K+LL + LGD    + Y  +FSNC+GYLR+  +TP  D+  +EE +E  
Sbjct: 129 AKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELVEGL 188

Query: 185 KKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAV 244
             HTD+S +TIV+Q+ + GLQMRSK G WID+    D  LVVNIGD M+AWSNGRLRS+ 
Sbjct: 189 GMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCND-FLVVNIGDLMQAWSNGRLRSSE 248

Query: 245 HRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENN-- 304
           HRVVLRK ++ R S+AF    EDE + I AP E+VGEGK R Y+SF   +Y  FR++N  
Sbjct: 249 HRVVLRK-LVNRVSLAFFLCFEDE-KVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSNEE 308

Query: 305 --YEKVGATIKDFVGI 313
             +EK+G T+KDF G+
Sbjct: 309 GKFEKIGFTVKDFAGL 316

[Close]

BLAST of CmaCh17G013420 vs. TAIR10
Match: AT5G51310.1 (AT5G51310.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 256.5 bits (654), Expect = 2.1e-68
Identity = 148/326 (45.40%), Postives = 214/326 (65.64%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           E+PV D+  + L+ES+ +SL  AC + GFF++ NHGVS++ Y+KL   +  +F   +E K
Sbjct: 4   ELPVFDIS-KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEEK 63

Query: 65  QQLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHAT--EFSNLLDEY 124
             +KMG S+YTPRF+ SPFFES RVSGP+F ASA +   +   F   AT  EFS L+ EY
Sbjct: 64  --MKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLMKEY 123

Query: 125 GRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFT-PLDM-----NAEEE 184
           G KM ++ ++++K +L+  GD++ +K+Y+ EF NC+GY RIN +T P D      N +E+
Sbjct: 124 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 183

Query: 185 --IEVFKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNG 244
             IE    HTD+S +TIV Q+DI GLQ+R++ G  +     +D  LVVN+GD + AW+NG
Sbjct: 184 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNG 243

Query: 245 RLRSAVHRVVLRKN--IMKRFSIAFLSTPEDESREIYAPSEVVG--EGKSRLYRSFSVQQ 304
           RLRS+ HRV+L++   +  RFS+AF     D+ + ++AP EVVG  EG  R++RSF    
Sbjct: 244 RLRSSQHRVILKRRGFVGNRFSLAFFWC-FDDGKVVFAPDEVVGGCEG-MRVFRSFKCGD 303

Query: 305 YKHFRENN----YEKVGATIKDFVGI 313
           Y  FRE+N    +EKVG T++DF  I
Sbjct: 304 YLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of CmaCh17G013420 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-25
Identity = 92/312 (29.49%), Postives = 149/312 (47.76%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTS-----SSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSS 64
           ++PVIDL   +  E          ++ A  + GFF I NHG+ K+ +E ++    +LF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 65  PEETKQQLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLL 124
           P   K + +    S            S+R   P  SA++ AQ      F    +E S + 
Sbjct: 99  PFSVKVRERFSDLSKN----------SYRWGNP--SATSPAQYSVSEAFHIILSEVSRIS 158

Query: 125 DEYGRKMKEVSK------RVVKLLLNVLGD--NIENKFYDPEFSNCNGYLRINGFTPLDM 184
           D+       V        RV +++  +LG   N+ +++++  F   N +LR+N + P   
Sbjct: 159 DDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVF 218

Query: 185 NAEEEIEVFKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAW 244
            +E  +     HTD S++TI+ Q+ I GL++ + G  WI V+   +  L VNIGD  +A 
Sbjct: 219 GSE--VFGLVPHTDTSFLTILSQDQIGGLELENNG-QWISVKPCLEA-LTVNIGDMFQAL 278

Query: 245 SNGRLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQY 304
           SNG  +S  HRV+   NI +R SIAF   P       Y  +E+   G  + YR FS ++Y
Sbjct: 279 SNGVYQSVRHRVISPANI-ERMSIAFFVCP-------YLETEIDCFGYPKKYRRFSFREY 326

[Close]

BLAST of CmaCh17G013420 vs. TAIR10
Match: AT1G80330.1 (AT1G80330.1 gibberellin 3-oxidase 4)

HSP 1 Score: 114.0 bits (284), Expect = 1.7e-25
Identity = 90/313 (28.75%), Postives = 153/313 (48.88%), Query Frame = 1


Query: 4   KEVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEET 63
           + +PVIDL    +T         A    G F I NHG+S++  + + S +  LF  P E 
Sbjct: 47  ESIPVIDLSNPDVTTLIGD----ASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSER 106

Query: 64  KQQLKM---GVSSY-TPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLL 123
           K +      GVS Y  PR  +SPFFE    S     A  + +    +L+ H  T++  ++
Sbjct: 107 KLEAASSDKGVSGYGEPR--ISPFFEKKMWSEGFTIADDSYRNHFNTLWPHDHTKYCGII 166

Query: 124 DEYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCN-----GYLRINGFTPLDMNAE 183
            EY  +M++++ R++  +L  LG  +E+  +  +          G +R+N + P+    E
Sbjct: 167 QEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY-PVCPEPE 226

Query: 184 EEIEVFKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNG 243
             + +   HTD + +TI+ Q++  GLQ+  +   W+ V  +  G+LVVNIGD     SNG
Sbjct: 227 RAMGL-AAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAP-GVLVVNIGDLFHILSNG 286

Query: 244 RLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHF 303
           ++ S VHR  +  +   R SIA+L        +I   S++ G  +  LYRS + ++Y   
Sbjct: 287 KIPSVVHRAKV-NHTRSRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRSITWKEYLQI 346

Query: 304 RENNYEKVGATIK 308
           +   ++K    I+
Sbjct: 347 KYEVFDKAMDAIR 349

[Close]

BLAST of CmaCh17G013420 vs. TAIR10
Match: AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)

HSP 1 Score: 111.3 bits (277), Expect = 1.1e-24
Identity = 92/314 (29.30%), Postives = 144/314 (45.86%), Query Frame = 1


Query: 6   VPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65
           VP IDL  Q  T      +  AC K GFF + NHGVS+          +  F  P   KQ
Sbjct: 63  VPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQ 122

Query: 66  --QLKMG-----VSSYTPRFLVS-PFFE--SFRVSGPNFSASAAAQGFTQSLFGHHATEF 125
             Q K G      SS+T RF    P+ E  SF+ S  N S S   Q +     G    +F
Sbjct: 123 KAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDN-SGSRTVQDYFSDTLGQEFEQF 182

Query: 126 SNLLDEYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEE 185
             +  +Y   M  +S ++++LL   LG N +  ++   F   +  +R+N + P      +
Sbjct: 183 GKVYQDYCEAMSSLSLKIMELLGLSLGVNRD--YFRGFFEENDSIMRLNHYPPCQ--TPD 242

Query: 186 EIEVFKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGR 245
                  H D S +TI+ Q+ + GLQ+      W  +R +     VVNIGD   A SNG 
Sbjct: 243 LTLGTGPHCDPSSLTILHQDHVNGLQV-FVDNQWQSIRPNPKAF-VVNIGDTFMALSNGI 302

Query: 246 LRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFR 305
            +S +HR V+ +   ++ S+AF   P+ + + +  PS+++ + K+R Y  F+   +  F 
Sbjct: 303 FKSCLHRAVVNRESARK-SMAFFLCPK-KDKVVKPPSDILEKMKTRKYPDFTWSMFLEFT 362

Query: 306 ENNYEKVGATIKDF 310
           + +Y     T+  F
Sbjct: 363 QKHYRADVNTLDSF 367

[Close]

BLAST of CmaCh17G013420 vs. NCBI nr
Match: gi|449458351|ref|XP_004146911.1| (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus])

HSP 1 Score: 352.4 bits (903), Expect = 7.9e-94
Identity = 178/314 (56.69%), Postives = 242/314 (77.07%), Query Frame = 1


Query: 4   KEVPVIDLG-CQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSS-PE 63
           +E+ V+D+  C+ L+ES+  SL +ACN+ G F+I+NHGVSKE Y+KL +  DEL ++ PE
Sbjct: 10  EELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPE 69

Query: 64  ETKQ-QLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 123
           ETK+ +LK+G S YTPRF +SP+ ESF+  GPNFS  A+  GFT+ +FG   T+F  LLD
Sbjct: 70  ETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLD 129

Query: 124 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVF 183
           EYG  M E+S+R++KLLL  +GDN+E+KFY+ EFSNCNGYLRIN + P   N+ EEIE F
Sbjct: 130 EYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAP--RNSNEEIEAF 189

Query: 184 KKHTDLSYVTIVFQNDIRGLQMRSKGGD-WIDVRLSEDGILVVNIGDYMEAWSNGRLRSA 243
            KHTD+S VTI+FQ++I G+QM+ K GD W+DVR  ED +L VNIGD+++AWSN RLRSA
Sbjct: 190 GKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALL-VNIGDFLQAWSNERLRSA 249

Query: 244 VHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENNY 303
            HRVVL++++ KRFS+AF    +D+ RE+YAPSEVVGEG +R+Y+ FS ++Y+ +RENNY
Sbjct: 250 EHRVVLKQDV-KRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 309

Query: 304 EK-VGATIKDFVGI 313
            K VG  +++F GI
Sbjct: 310 RKIVGVPLREFAGI 319

[Close]

BLAST of CmaCh17G013420 vs. NCBI nr
Match: gi|659107869|ref|XP_008453897.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo])

HSP 1 Score: 351.3 bits (900), Expect = 1.8e-93
Identity = 180/314 (57.32%), Postives = 241/314 (76.75%), Query Frame = 1


Query: 4   KEVPVIDLGC-QSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSS-PE 63
           +E+PV+D+ C + L+ES+  SL +ACN+ G F+I+NHGVSKE Y KL +  DEL ++ PE
Sbjct: 10  EELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAVPE 69

Query: 64  ETKQ-QLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 123
           E K+ +LK+GVS YTPRF +SP+ ESF+  GP FS SA+  GFT+ +FGH  T+F NLLD
Sbjct: 70  EAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQFRNLLD 129

Query: 124 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVF 183
           EYG  M E+S+R++KLLL ++GD+ E+KFY+ EFSNCNGY+RIN + P   N+ EEIE F
Sbjct: 130 EYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAP--RNSNEEIEAF 189

Query: 184 KKHTDLSYVTIVFQNDIRGLQMRSKGGD-WIDVRLSEDGILVVNIGDYMEAWSNGRLRSA 243
            KHTD+S VTI+FQ++I G+QM+SK GD W+DVR  ED +L VNIGD+++AWSN RLRSA
Sbjct: 190 GKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALL-VNIGDFLQAWSNERLRSA 249

Query: 244 VHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENNY 303
            HRVVL++N  KRF++ F     D+ RE+YAPSEVVGEG  R+Y+ FS ++Y+ FRENNY
Sbjct: 250 EHRVVLKQN-AKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENNY 309

Query: 304 EK-VGATIKDFVGI 313
            K VG  +++F GI
Sbjct: 310 GKVVGVPMREFAGI 319

[Close]

BLAST of CmaCh17G013420 vs. NCBI nr
Match: gi|659116735|ref|XP_008458231.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo])

HSP 1 Score: 293.9 bits (751), Expect = 3.3e-76
Identity = 158/318 (49.69%), Postives = 218/318 (68.55%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           E+P++D+  Q L+ S+ SSL  AC K GFF I+NHG+SKE Y+KL S ++E+FS P ETK
Sbjct: 9   ELPILDIS-QPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIFSLPLETK 68

Query: 65  QQLKMG----VSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHAT-EFSNLL 124
             LK+G    +++YTP F+ SPFFE+ RVSGPNF ASA  Q   + LF   ++ +FS +L
Sbjct: 69  --LKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASA--QNSAEFLFNQKSSHQFSEIL 128

Query: 125 DEYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLD-MNAEEEIE 184
            EYG KM E+S++++K +L   G+  E K+++P F NC+GYLRIN +TP + +  +EE E
Sbjct: 129 QEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEEAE 188

Query: 185 VFKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDG-ILVVNIGDYMEAWSNGRLR 244
               HTD+S VTIV+Q+   GLQ++S  G W+D+   E+   LVVNIGD ++AWSN RLR
Sbjct: 189 GLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSNDRLR 248

Query: 245 SAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFREN 304
           S+ HRVVLR+  + RFSIAF    EDE + + AP E+VGEG  R+Y+ F    Y  FREN
Sbjct: 249 SSEHRVVLRRPAINRFSIAFFWGFEDE-KVVSAPKELVGEGGKRMYKPFVCSDYLKFREN 308

Query: 305 N----YEKVGATIKDFVG 312
           +    +EKVG T++DF G
Sbjct: 309 DERGKFEKVGFTVRDFAG 320

[Close]

BLAST of CmaCh17G013420 vs. NCBI nr
Match: gi|661887686|emb|CDP08817.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 293.5 bits (750), Expect = 4.3e-76
Identity = 161/317 (50.79%), Postives = 218/317 (68.77%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           ++P +D+  + L   + SSL  AC++ GFF I NHG+SKE Y+K++S + +LFS P ETK
Sbjct: 10  KLPSLDIS-KPLDSCSLSSLTAACSEWGFFQITNHGISKELYDKILSLSKDLFSLPSETK 69

Query: 65  QQLKMG----VSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 124
             LK+G    + +YTP F+ SPFFES RVSGP+F+ASA  Q   + L   + +EFS +L 
Sbjct: 70  --LKLGPFSSLKTYTPHFIASPFFESLRVSGPDFAASA--QDSAEVLSAPNISEFSEILH 129

Query: 125 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLD-MNAEEEIEV 184
           EYGRKM E+SK V+KL+L  LG   E K+Y+ EFSNC GYLRIN + P + +  EEE E 
Sbjct: 130 EYGRKMTEISKEVLKLVLMSLGAEFEMKYYNSEFSNCQGYLRINNYAPPESLEEEEEAEG 189

Query: 185 FKKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSA 244
              HTD+S +TIV+Q+++ GLQ++S+ G W+D+ +  +G LVVNIGD ++AWSN + RS+
Sbjct: 190 LGMHTDMSCITIVYQDEVGGLQVKSREGKWMDI-VPCEGTLVVNIGDVLQAWSNDKFRSS 249

Query: 245 VHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENN- 304
            HRVVLRK ++ RFS+AF    EDE + I AP EVVG   SR+Y+ F    Y  FRENN 
Sbjct: 250 EHRVVLRK-LVNRFSLAFFWCFEDE-KIILAPDEVVGRENSRIYKPFVCSDYLKFRENNE 309

Query: 305 ---YEKVGATIKDFVGI 313
              +EKVG T+KDF GI
Sbjct: 310 KGKFEKVGFTVKDFAGI 318

[Close]

BLAST of CmaCh17G013420 vs. NCBI nr
Match: gi|255539839|ref|XP_002510984.1| (PREDICTED: gibberellin 2-beta-dioxygenase 7 [Ricinus communis])

HSP 1 Score: 291.6 bits (745), Expect = 1.6e-75
Identity = 163/313 (52.08%), Postives = 218/313 (69.65%), Query Frame = 1


Query: 5   EVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETK 64
           ++P +D+  Q L++S+ SSL  AC + GFF I NHGVSK+ Y KL   AD LF+ P E+K
Sbjct: 9   QLPALDIS-QPLSQSSLSSLSSACKEWGFFRIINHGVSKDLYRKLYFLADHLFNLPCESK 68

Query: 65  QQLKMGVSS----YTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLD 124
             +K+G SS    YTP F+ SPFFES RVSGP+F ASA  Q  +Q LF H   EFS  + 
Sbjct: 69  --IKIGPSSSIKTYTPHFIASPFFESLRVSGPDFFASA--QTSSQVLFHHPNPEFSEAMS 128

Query: 125 EYGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVF 184
           EYG KM E+SKR+VK +L  +GD+   KFY+ +F+ C+GY+RIN ++P +  + EE+E  
Sbjct: 129 EYGNKMSELSKRIVKAVLISMGDDFAEKFYESDFNKCHGYMRINKYSPAE--SAEEVEGL 188

Query: 185 KKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAV 244
             HTD+S +TIV Q+DI GLQ+RSK G W+D+   ED  LVVNIGD M AWSNG+LRS+ 
Sbjct: 189 GMHTDMSCMTIVCQDDIGGLQVRSKLGKWMDINPCED-TLVVNIGDLMHAWSNGKLRSSE 248

Query: 245 HRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENN-- 304
           HRVVLR+ +  RFS+AF    ED+ + I+AP+EVVGEG  R+Y+ F  + Y +FRE++  
Sbjct: 249 HRVVLRRQV-HRFSLAFFWCFEDD-KVIFAPNEVVGEGNLRMYKPFVCRDYLNFRESSEK 308

Query: 305 --YEKVGATIKDF 310
             ++KVG TIKDF
Sbjct: 309 GKFDKVGFTIKDF 311

The following BLAST results are available for this feature:

BLAST of CmaCh17G013420 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G2OX7_ARATH	2.3e-24	29.49	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1	[more]
G3OX_PEA	3.3e-23	28.53	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1	[more]
GAOX5_ARATH	7.3e-23	28.04	Gibberellin 20 oxidase 5 OS=Arabidopsis thaliana GN=GA20OX5 PE=2 SV=1	[more]
YQK1_SCHPO	1.2e-22	29.82	UPF0676 protein C1494.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ...	[more]
GAOX1_ARATH	2.8e-22	29.50	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2	[more]

BLAST of CmaCh17G013420 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KW97_CUCSA	5.5e-94	56.69	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G022900 PE=3 SV=1	[more]
A0A068UKJ5_COFCA	3.0e-76	50.79	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00027923001 PE=3 SV=1	[more]
B9R9V9_RICCO	1.1e-75	52.08	Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1500930 PE=3 SV=1	[more]
A0A061FZ75_THECC	1.5e-75	51.75	2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao GN=TCM_014890 PE=3 SV=1	[more]
D7TVY9_VITVI	4.4e-75	49.37	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03440 PE=3 SV=...	[more]

BLAST of CmaCh17G013420 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G23340.1	4.9e-78	50.32	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G51310.1	2.1e-68	45.40	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G50960.1	1.3e-25	29.49	gibberellin 2-oxidase 7	[more]
AT1G80330.1	1.7e-25	28.75	gibberellin 3-oxidase 4	[more]
AT5G51810.1	1.1e-24	29.30	gibberellin 20 oxidase 2	[more]

BLAST of CmaCh17G013420 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449458351\|ref\|XP_004146911.1\|	7.9e-94	56.69	PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus]	[more]
gi\|659107869\|ref\|XP_008453897.1\|	1.8e-93	57.32	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo]	[more]
gi\|659116735\|ref\|XP_008458231.1\|	3.3e-76	49.69	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo]	[more]
gi\|661887686\|emb\|CDP08817.1\|	4.3e-76	50.79	unnamed protein product [Coffea canephora]	[more]
gi\|255539839\|ref\|XP_002510984.1\|	1.6e-75	52.08	PREDICTED: gibberellin 2-beta-dioxygenase 7 [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR026992	DIOX_N
IPR027443	IPNS-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh17G013420.1	CmaCh17G013420.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 180..260 score: 6.6
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 165..261 score: 9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 6..69 score: 7.8
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 4..300 score: 3.7
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 5..306 score: 2.6E
None	No IPR available	PANTHER	PTHR10209:SF151	SUBFAMILY NOT NAMED	coord: 5..306 score: 2.6E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 2..298 score: 2.33

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh17G013420	Watermelon (97103) v2	cmawmbB390
CmaCh17G013420	Wax gourd	cmawgoB0493
CmaCh17G013420	Cucurbita maxima (Rimu)	cmacmaB374
CmaCh17G013420	Cucumber (Gy14) v1	cgycmaB1077
CmaCh17G013420	Cucurbita moschata (Rifu)	cmacmoB353
CmaCh17G013420	Cucurbita moschata (Rifu)	cmacmoB366
CmaCh17G013420	Wild cucumber (PI 183967)	cmacpiB388
CmaCh17G013420	Cucumber (Chinese Long) v2	cmacuB384
CmaCh17G013420	Melon (DHL92) v3.5.1	cmameB322
CmaCh17G013420	Watermelon (Charleston Gray)	cmawcgB309
CmaCh17G013420	Watermelon (97103) v1	cmawmB331
CmaCh17G013420	Cucurbita pepo (Zucchini)	cmacpeB371
CmaCh17G013420	Cucurbita pepo (Zucchini)	cmacpeB392
CmaCh17G013420	Bottle gourd (USVL1VR-Ls)	cmalsiB346
CmaCh17G013420	Cucumber (Gy14) v2	cgybcmaB920
CmaCh17G013420	Melon (DHL92) v3.6.1	cmamedB374
CmaCh17G013420	Silver-seed gourd	carcmaB0020
CmaCh17G013420	Silver-seed gourd	carcmaB0104
CmaCh17G013420	Cucumber (Chinese Long) v3	cmacucB0457