CmaCh17G013400 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh17G013400
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cma_Chr17 : 8997237 .. 8998317 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGATAGAGGAAGTTCCCGAGATAGACATTAGCTCCCAGAGCTTGACCGAATCGACTGCATCTTCTCTATCCAAAGCGTGCACTGAATTGGAATTTTTCTTCATAAAAAACCATGGCATTTCGAAAGAGTTTTACTATAAACTCATTTCCGGCATTGACCAGCTTTTTTCTTCTCCCGAGGAGACCAAGCAAAAGCTAAAACAAAGAGTTTCCTCATACACCCCAAGATTTGTGGTCTCGCCTTTTTTGGAGAGCTTCAAGCTTTCAGGACCCAAGTTTTCAGTCTTTGCTCGTGTGCAAGGTTTCACTCAATCCTAATTCGGCCACCAAGCTACTGAATTCAGGTCATCTTTCTTAAATTTATCTCCAGTAGAACTACAACTTTTGAATGTTGTGTTCCGATATATTCATGAAAGAATTATACAATCAACCATATTCATCATCTTCTTCTTTATTTTGAGTTTGTATGTTCGAGTTTATTTGCAGCAATCTTCTAGACGAATATGGAAGCAAAATGACAGAAGTATCGAAAAAAGTAGTGAAGCTGTTACTGAAGATCTTGGGAGTTAGTATTGAAAATAAATTCTATGATCTCGAGTTTAGCAACTGCAATAGCTACCTAAGAATAAACGACTACACTCCTCTAGGCATGAATGATGAAGAAGAAGTCGAAGTGTTCCAGAAGCACACCGACCTCAGCTTTGTGACGATAGTGTTCCCAAATGACATCAGGGGGTTACAAATGAGATCTAAAGGAGGCAACTGGATCGACGTAAGGCCTAGCGAGGACGGTAATCTTATAGTGAACATTGGAGATTATATGGAGGCTTGGAGCAACAGGAGGCTGAGATCTGCCGTACATAGAGTTGTTTTAAGGAAAAATATTATGAAGAGATTCTCTATCGCTTTCTTATCGACACCGGAAGATGAATCTAGAGAAATATATGCACCGAGTGAAGTCGTTGGAGAAGGAAAATCAAGACTTTACCGCTCGTTTTCAATCCAACAATATAAGCATTTTAGAGAAAACAATTACGAGAACGTTGGAGCCACAATTAAGGATTTTGTTGGAATATGA

mRNA sequence

ATGTCGATAGAGGAAGTTCCCGAGATAGACATTAGCTCCCAGAGCTTGACCGAATCGACTGCATCTTCTCTATCCAAAGCGTGCACTGAATTGGAATTTTTCTTCATAAAAAACCATGGCATTTCGAAAGAGTTTTACTATAAACTCATTTCCGGCATTGACCAGCTTTTTTCTTCTCCCGAGGAGACCAAGCAAAAGCTAAAACAAAGAGTTTCCTCATACACCCCAAGATTTGTGGTCTCGCCTTTTTTGGAGAGCTTCAAGCTTTCAGGACCCAAGTTTTCAGTCTTTGCTCGTGTGCAAGTTTGTATGTTCGAGTTTATTTGCAGCAATCTTCTAGACGAATATGGAAGCAAAATGACAGAAGTATCGAAAAAAGTAGTGAAGCTGTTACTGAAGATCTTGGGAGTTAGTATTGAAAATAAATTCTATGATCTCGAGTTTAGCAACTGCAATAGCTACCTAAGAATAAACGACTACACTCCTCTAGGCATGAATGATGAAGAAGAAGTCGAAGTGTTCCAGAAGCACACCGACCTCAGCTTTGTGACGATAGTGTTCCCAAATGACATCAGGGGGTTACAAATGAGATCTAAAGGAGGCAACTGGATCGACGTAAGGCCTAGCGAGGACGGTAATCTTATAGTGAACATTGGAGATTATATGGAGGCTTGGAGCAACAGGAGGCTGAGATCTGCCGTACATAGAGTTGTTTTAAGGAAAAATATTATGAAGAGATTCTCTATCGCTTTCTTATCGACACCGGAAGATGAATCTAGAGAAATATATGCACCGAGTGAAGTCGTTGGAGAAGGAAAATCAAGACTTTACCGCTCGTTTTCAATCCAACAATATAAGCATTTTAGAGAAAACAATTACGAGAACGTTGGAGCCACAATTAAGGATTTTGTTGGAATATGA

Coding sequence (CDS)

ATGTCGATAGAGGAAGTTCCCGAGATAGACATTAGCTCCCAGAGCTTGACCGAATCGACTGCATCTTCTCTATCCAAAGCGTGCACTGAATTGGAATTTTTCTTCATAAAAAACCATGGCATTTCGAAAGAGTTTTACTATAAACTCATTTCCGGCATTGACCAGCTTTTTTCTTCTCCCGAGGAGACCAAGCAAAAGCTAAAACAAAGAGTTTCCTCATACACCCCAAGATTTGTGGTCTCGCCTTTTTTGGAGAGCTTCAAGCTTTCAGGACCCAAGTTTTCAGTCTTTGCTCGTGTGCAAGTTTGTATGTTCGAGTTTATTTGCAGCAATCTTCTAGACGAATATGGAAGCAAAATGACAGAAGTATCGAAAAAAGTAGTGAAGCTGTTACTGAAGATCTTGGGAGTTAGTATTGAAAATAAATTCTATGATCTCGAGTTTAGCAACTGCAATAGCTACCTAAGAATAAACGACTACACTCCTCTAGGCATGAATGATGAAGAAGAAGTCGAAGTGTTCCAGAAGCACACCGACCTCAGCTTTGTGACGATAGTGTTCCCAAATGACATCAGGGGGTTACAAATGAGATCTAAAGGAGGCAACTGGATCGACGTAAGGCCTAGCGAGGACGGTAATCTTATAGTGAACATTGGAGATTATATGGAGGCTTGGAGCAACAGGAGGCTGAGATCTGCCGTACATAGAGTTGTTTTAAGGAAAAATATTATGAAGAGATTCTCTATCGCTTTCTTATCGACACCGGAAGATGAATCTAGAGAAATATATGCACCGAGTGAAGTCGTTGGAGAAGGAAAATCAAGACTTTACCGCTCGTTTTCAATCCAACAATATAAGCATTTTAGAGAAAACAATTACGAGAACGTTGGAGCCACAATTAAGGATTTTGTTGGAATATGA

Protein sequence

MSIEEVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETKQKLKQRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMFEFICSNLLDEYGSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYTPLGMNDEEEVEVFQKHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENNYENVGATIKDFVGI

Homology

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BLAST of CmaCh17G013400 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.9e-21
Identity = 92/312 (29.49%), Postives = 149/312 (47.76%), Query Frame = 1


Query: 5   EVPEIDISSQSLTEST-----ASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSS 64
           ++P ID+S  +  E          +  A  E  FF I NHGI K+ +  ++    +LF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 65  PEETKQKLKQRVSSYTPRFVVSPFLESFKLSGP------KFSVFARVQVCMFEFICSNLL 124
           P   K  +++R S  +          S++   P      ++SV     + + E   S + 
Sbjct: 99  PFSVK--VRERFSDLSKN--------SYRWGNPSATSPAQYSVSEAFHIILSEV--SRIS 158

Query: 125 DEYGSKMTEVSK------KVVKLLLKILG--VSIENKFYDLEFSNCNSYLRINDYTPLGM 184
           D+  +  T V        +V +++ +ILG  V++ +++++  F   NS+LR+N Y P   
Sbjct: 159 DDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVF 218

Query: 185 NDEEEVEVFQKHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAW 244
             E  V     HTD SF+TI+  + I GL++ + G  WI V+P  +  L VNIGD  +A 
Sbjct: 219 GSE--VFGLVPHTDTSFLTILSQDQIGGLELENNG-QWISVKPCLEA-LTVNIGDMFQAL 278

Query: 245 SNRRLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQY 298
           SN   +S  HRV+   NI +R SIAF   P       Y  +E+   G  + YR FS ++Y
Sbjct: 279 SNGVYQSVRHRVISPANI-ERMSIAFFVCP-------YLETEIDCFGYPKKYRRFSFREY 326

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BLAST of CmaCh17G013400 vs. Swiss-Prot
Match: G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.5e-20
Identity = 85/308 (27.60%), Postives = 145/308 (47.08%), Query Frame = 1


Query: 2   SIEEVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPE 61
           S   VP ID++  + ++     +  AC     + + NHGI       +      LFS P 
Sbjct: 52  STTTVPVIDLNDPNASKL----IGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPS 111

Query: 62  ETKQKLKQ-----------RVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMFEFICS 121
             K K  +           R+SS+ P+ +   + E F + G     F  +    +   C 
Sbjct: 112 HQKHKATRSPDGVSGYGIARISSFFPKLM---WYEGFTIVGSPLDHFRELWPQDYTRFC- 171

Query: 122 NLLDEYGSKMTEVSKKVVKLLLKILGVSIENKFY---DLEFSNCNSYLRINDYTPLGMND 181
           +++ +Y   M +++  ++ L+L  LG++ E+  +     +F    + L++N Y      D
Sbjct: 172 DIVVQYDETMKKLAGTLMCLMLDSLGITKEDIKWAGSKAQFEKACAALQLNSYP--SCPD 231

Query: 182 EEEVEVFQKHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSN 241
            +       HTD +F+TI+  NDI GLQ+  +G  WI V P + G L+VN+GD     SN
Sbjct: 232 PDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQ-GGLVVNVGDLFHILSN 291

Query: 242 RRLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQY-- 291
               S +HRV++ +   +RFS+A+L  P   + EI   ++++G  K  LYRS +  +Y  
Sbjct: 292 GLYPSVLHRVLVNRT-RQRFSVAYLYGP-PSNVEICPHAKLIGPTKPPLYRSVTWNEYLG 346

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BLAST of CmaCh17G013400 vs. Swiss-Prot
Match: F6H2_ARATH (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 5.6e-20
Identity = 100/313 (31.95%), Postives = 149/313 (47.60%), Query Frame = 1


Query: 2   SIEEVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPE 61
           S E +P IDIS+    +S + ++  A  E  FF + NHG+S E    + +   + F  P 
Sbjct: 58  SDESIPVIDISNLD-EKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPV 117

Query: 62  ETKQKLKQRVS-SYTPRFVVS--PFLESFKLSGPKFSVFARVQVCMFEFICSNLLDEYGS 121
           E K+K  +  S S   RF  S  P  E         S+F   +    +    +   E   
Sbjct: 118 EEKRKFSREKSLSTNVRFGTSFSPHAEKALEWKDYLSLFFVSEAEASQLWPDSCRSETLE 177

Query: 122 KMTEVSKKVVKLLLKILGVSIENKFYD-LEFSNCNSYLRIN-DYTPLGMNDEEEVEVFQK 181
            M E +K +VK LL+ LG ++  K  D  + S      RIN +Y P+  N E  V V  +
Sbjct: 178 YMNE-TKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPNPELTVGV-GR 237

Query: 182 HTDLSFVTIVFPNDIRGLQMRS-KGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVH 241
           H+D+S +TI+  ++I GL +RS   G W+ V P   G+L++NIGD M+  SN R +S  H
Sbjct: 238 HSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPIS-GSLVINIGDAMQIMSNGRYKSVEH 297

Query: 242 RVVLRKNIMKRFSIAFLSTPEDESREIYAP-SEVVGEGKSRLYRSFSIQQY-KHFRENNY 301
           R VL      R S+    +P+ ES  +  P  EV+  G+  +Y+      Y KHF    +
Sbjct: 298 R-VLANGSYNRISVPIFVSPKPES--VIGPLLEVIENGEKPVYKDILYTDYVKHFFRKAH 357

Query: 302 ENVGATIKDFVGI 307
           +  G    DF  I
Sbjct: 358 D--GKKTIDFANI 361

[Close]

BLAST of CmaCh17G013400 vs. Swiss-Prot
Match: GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.4e-20
Identity = 81/311 (26.05%), Postives = 141/311 (45.34%), Query Frame = 1


Query: 6   VPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETKQ 65
           VP ID+SSQ  T      +++ACT+  FF + NHG+S+         ++  F  P   KQ
Sbjct: 63  VPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQ 122

Query: 66  KLKQR-------VSSYTPRFVVS-PFLESFKLSGPKFSVFARVQVCMFEFICSNLLDEYG 125
           K +++        SS+T RF    P+ E+        +  +R     F        +++G
Sbjct: 123 KAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLGQEFEQFG 182

Query: 126 SKMTEVSKKVVKLLLKI-----LGVSIENKFYDLEFSNCNSYLRINDYTPLGMNDEEEVE 185
               +  + +  L LKI     L + +   ++   F   +S +R+N Y P    D     
Sbjct: 183 KVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPD--LTL 242

Query: 186 VFQKHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRS 245
               H D S +TI+  + + GLQ+      W  +RP+     +VNIGD   A SN   +S
Sbjct: 243 GTGPHCDPSSLTILHQDHVNGLQV-FVDNQWQSIRPNPKA-FVVNIGDTFMALSNGIFKS 302

Query: 246 AVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN 304
            +HR V+ +   ++ S+AF   P+ + + +  PS+++ + K+R Y  F+   +  F + +
Sbjct: 303 CLHRAVVNRESARK-SMAFFLCPK-KDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKH 362

[Close]

BLAST of CmaCh17G013400 vs. Swiss-Prot
Match: F6H1_ARATH (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.3e-19
Identity = 95/296 (32.09%), Postives = 145/296 (48.99%), Query Frame = 1


Query: 4   EEVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEET 63
           E +P ID+S+    +  A ++  A  +  FF + NHG+  E    + +   + F+ P E 
Sbjct: 60  EAIPVIDMSNPD-EDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNLPVEE 119

Query: 64  KQKL-KQRVSSYTPRFVVS--PFLESFKLSGPKFSVFARVQVCMFEF---ICSNLLDEYG 123
           K+K  K+   S T RF  S  P  E         S+F   +    +F   IC N   EY 
Sbjct: 120 KRKFTKENSLSTTVRFGTSFSPLAEQALEWKDYLSLFFVSEAEAEQFWPDICRNETLEYI 179

Query: 124 SKMTEVSKKVVKLLLKILGVSIENKFYD-LEFSNCNSYLRIN-DYTPLGMNDEEEVEVFQ 183
           +K    SKK+V+ LL+ LG ++  K  D  + S     +R+N +Y P+  N +  V V  
Sbjct: 180 NK----SKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYPICPNPDLTVGV-G 239

Query: 184 KHTDLSFVTIVFPNDIRGLQMRS-KGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAV 243
           +H+D+S +TI+  + I GL +RS   GNW+ V P   G+ ++NIGD M+  SN   +S  
Sbjct: 240 RHSDVSSLTILLQDQIGGLHVRSLASGNWVHV-PPVAGSFVINIGDAMQIMSNGLYKSVE 299

Query: 244 HRVVLRKNIMKRFSIAFLSTPEDESREIYAP-SEVVGEGKSRLYRSFSIQQY-KHF 289
           HR VL      R S+     P+ ES  +  P  EV+  G+  +YR      Y K+F
Sbjct: 300 HR-VLANGYNNRISVPIFVNPKPES--VIGPLPEVIANGEEPIYRDVLYSDYVKYF 345

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BLAST of CmaCh17G013400 vs. TrEMBL
Match: A0A0A0KW97_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 5.8e-80
Identity = 168/318 (52.83%), Postives = 227/318 (71.38%), Query Frame = 1


Query: 4   EEVPEIDISS-QSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSS-PE 63
           EE+  +D++S + L+ES+  SL KAC E   F+IKNHG+SKE Y KL +  D+L ++ PE
Sbjct: 10  EELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPE 69

Query: 64  ETKQ-KLKQRVSSYTPRFVVSPFLESFKLSGPKFSVFAR----------VQVCMFEFICS 123
           ETK+ KLK   S YTPRF +SP++ESFK  GP FS +A            +V  F     
Sbjct: 70  ETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFR---- 129

Query: 124 NLLDEYGSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYTPLGMNDEEE 183
            LLDEYGS M E+S++++KLLLK +G ++E+KFY+ EFSNCN YLRIN Y P   N  EE
Sbjct: 130 KLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAP--RNSNEE 189

Query: 184 VEVFQKHTDLSFVTIVFPNDIRGLQMRSK-GGNWIDVRPSEDGNLIVNIGDYMEAWSNRR 243
           +E F KHTD+S VTI+F ++I G+QM+ K G  W+DVRP ED  L+VNIGD+++AWSN R
Sbjct: 190 IEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDA-LLVNIGDFLQAWSNER 249

Query: 244 LRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFR 303
           LRSA HRVVL++++ KRFS+AF    +D+ RE+YAPSEVVGEG +R+Y+ FS ++Y+ +R
Sbjct: 250 LRSAEHRVVLKQDV-KRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYR 309

Query: 304 ENNYEN-VGATIKDFVGI 307
           ENNY   VG  +++F GI
Sbjct: 310 ENNYRKIVGVPLREFAGI 319

[Close]

BLAST of CmaCh17G013400 vs. TrEMBL
Match: A0A068UKJ5_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00027923001 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 5.4e-70
Identity = 155/315 (49.21%), Postives = 206/315 (65.40%), Query Frame = 1


Query: 5   EVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETK 64
           ++P +DIS + L   + SSL+ AC+E  FF I NHGISKE Y K++S    LFS P ETK
Sbjct: 10  KLPSLDIS-KPLDSCSLSSLTAACSEWGFFQITNHGISKELYDKILSLSKDLFSLPSETK 69

Query: 65  QKLK--QRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMF------EFICSNLLDEY 124
            KL     + +YTP F+ SPF ES ++SGP F+  A+    +       EF  S +L EY
Sbjct: 70  LKLGPFSSLKTYTPHFIASPFFESLRVSGPDFAASAQDSAEVLSAPNISEF--SEILHEY 129

Query: 125 GSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYT-PLGMNDEEEVEVFQ 184
           G KMTE+SK+V+KL+L  LG   E K+Y+ EFSNC  YLRIN+Y  P  + +EEE E   
Sbjct: 130 GRKMTEISKEVLKLVLMSLGAEFEMKYYNSEFSNCQGYLRINNYAPPESLEEEEEAEGLG 189

Query: 185 KHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVH 244
            HTD+S +TIV+ +++ GLQ++S+ G W+D+ P E G L+VNIGD ++AWSN + RS+ H
Sbjct: 190 MHTDMSCITIVYQDEVGGLQVKSREGKWMDIVPCE-GTLVVNIGDVLQAWSNDKFRSSEH 249

Query: 245 RVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN--- 304
           RVVLRK ++ RFS+AF    EDE + I AP EVVG   SR+Y+ F    Y  FRENN   
Sbjct: 250 RVVLRK-LVNRFSLAFFWCFEDE-KIILAPDEVVGRENSRIYKPFVCSDYLKFRENNEKG 309

Query: 305 -YENVGATIKDFVGI 307
            +E VG T+KDF GI
Sbjct: 310 KFEKVGFTVKDFAGI 318

[Close]

BLAST of CmaCh17G013400 vs. TrEMBL
Match: A0A061FZ75_THECC (2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao GN=TCM_014890 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 6.0e-69
Identity = 154/313 (49.20%), Postives = 206/313 (65.81%), Query Frame = 1


Query: 5   EVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETK 64
           E+P  D+S Q L+ S+ SSLS AC E  FF I NHG+SK+ Y KL S  + LFS P+E+K
Sbjct: 9   ELPVFDLS-QPLSPSSLSSLSLACREWGFFRITNHGVSKDLYRKLYSLSNHLFSLPDESK 68

Query: 65  QKL--KQRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMF------EFICSNLLDEY 124
            KL     + +YTP F+ SPF ES ++SGP F   A+  V +       EF  S +L EY
Sbjct: 69  LKLGPTSSLKTYTPHFIASPFFESLRVSGPDFFASAQSSVDVLFEQPNSEF--SEVLKEY 128

Query: 125 GSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYTPLGMNDEEEVEVFQK 184
           GS MT++SK++V+ +LK LG  +E K ++ EF NC+ YLRIN+YTP     EEEV+    
Sbjct: 129 GSLMTKLSKEIVEAILKSLGEELEKKSFESEFGNCHGYLRINNYTPPKSMKEEEVDGLGM 188

Query: 185 HTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVHR 244
           HTD+S VTIV+ ++I GLQ+RSK G W+D+ P ED  L+VNIGD M AWSN +L S+ HR
Sbjct: 189 HTDMSCVTIVYQDEIGGLQVRSKEGKWMDINPCED-TLVVNIGDLMHAWSNGKLSSSEHR 248

Query: 245 VVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN---- 304
           VVL K I+ RFS+AF    EDE + I+AP+EVVGE   R+Y+ F    Y  FR+++    
Sbjct: 249 VVL-KRIVNRFSLAFFWCFEDE-KVIFAPNEVVGEDNLRIYKPFVCADYLKFRQSSEKGR 308

Query: 305 YENVGATIKDFVG 306
           ++ VG T+KDF G
Sbjct: 309 FDKVGFTVKDFAG 315

[Close]

BLAST of CmaCh17G013400 vs. TrEMBL
Match: D7TVY9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03440 PE=3 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.2e-66
Identity = 146/314 (46.50%), Postives = 200/314 (63.69%), Query Frame = 1


Query: 5   EVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETK 64
           ++P +D+S Q +  S  +SLS+AC E  FF + NHGI ++ + K+      +FS P ++K
Sbjct: 9   QLPVLDLS-QPVHPSFLTSLSQACEEWGFFHVMNHGIPQDLFSKVCLLSKHIFSLPADSK 68

Query: 65  QKL--KQRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMF------EFICSNLLDEY 124
            KL     + +YTPRF+ SPF ES +++GP F   A+  V         EF  S  L EY
Sbjct: 69  LKLGPSSSIKTYTPRFIASPFFESLRIAGPDFLASAKSSVAELFDQQNSEF--SETLQEY 128

Query: 125 GSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYTPLGMNDEEEVEVFQK 184
           GSKM E+SK++V+ +L  LG   E KF + EF+NC  YLR+ +Y+P    ++EEVE    
Sbjct: 129 GSKMVELSKRIVEAVLMSLGDGYERKFNESEFNNCQGYLRLVNYSPPECVEDEEVEGLGM 188

Query: 185 HTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVHR 244
           HTD+S +TIV+ ++I GLQMRSKGG WID+ P E G L+VNIGD M+ WSN RLRS+ HR
Sbjct: 189 HTDMSCITIVYQDEIGGLQMRSKGGEWIDITPCE-GTLVVNIGDLMQGWSNGRLRSSEHR 248

Query: 245 VVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN---- 304
           VVL K  + R S+AF    EDE + + AP E+VGEG SR+Y+ F   +Y  FRE+N    
Sbjct: 249 VVL-KRFVNRLSLAFFWCFEDE-KVVLAPDEIVGEGNSRIYKPFVCLEYLKFRESNEEGK 308

Query: 305 YENVGATIKDFVGI 307
           YE VG T+KDF  +
Sbjct: 309 YEKVGYTVKDFAAL 316

[Close]

BLAST of CmaCh17G013400 vs. TrEMBL
Match: A0A0A0KB92_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G004480 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.6e-66
Identity = 146/317 (46.06%), Postives = 203/317 (64.04%), Query Frame = 1


Query: 5   EVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETK 64
           E+P +DIS Q L+ S+ SSL+ AC +  FF IKNHGISKE Y KL S  +++F+ P ETK
Sbjct: 9   ELPILDIS-QPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSETK 68

Query: 65  QKLK--QRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMFEFIC--------SNLLD 124
            K+     V++YTP F+ SPF E+ ++SGP F   A+      EF+         S +L 
Sbjct: 69  LKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSA---EFLFNQKSSHQFSEILQ 128

Query: 125 EYGSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYT-PLGMNDEEEVEV 184
           EYG KMTE+S+K++K +L   G   E K+++  F NC+ YLRIN+YT P  + D+EEVE 
Sbjct: 129 EYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEEVEG 188

Query: 185 FQKHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGN-LIVNIGDYMEAWSNRRLRS 244
              HTD+S VTIV+ +   GLQ++S  G W+D+ P E+   L+VNIGD ++AWSN R+RS
Sbjct: 189 LGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSNDRVRS 248

Query: 245 AVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN 304
           + HRVVLR+  + RFSIAF    EDE + + AP ++V EG  R+Y+ F    Y  FREN+
Sbjct: 249 SEHRVVLRRPAINRFSIAFFWGFEDE-KVVSAPEDLVEEGGERMYKPFVCSDYLKFREND 308

Query: 305 ----YENVGATIKDFVG 306
               +E VG T+ +F G
Sbjct: 309 ERGKFEKVGFTVTNFAG 320

[Close]

BLAST of CmaCh17G013400 vs. TAIR10
Match: AT4G23340.1 (AT4G23340.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 251.5 bits (641), Expect = 6.5e-67
Identity = 144/314 (45.86%), Postives = 200/314 (63.69%), Query Frame = 1


Query: 5   EVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETK 64
           ++P +D++ Q +  S  SSLS+AC E  FF++ NHGISKE + K+ S    +F +P E+K
Sbjct: 9   QLPVLDLT-QPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKAPLESK 68

Query: 65  QKLKQRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMF-----EFICSNLLDEYGSK 124
            KL     SYTPR++ SP+ ES  +SGP FS  A+    +      +      + EYG+K
Sbjct: 69  LKLGP--ISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELRETMQEYGAK 128

Query: 125 MTEVSKKVVKLLLKI-LGVSIENKFYDLEFSNCNSYLRINDYTPLG--MNDEEEVEVFQK 184
           M E+SK+++K+LL + LG     + Y  +FSNC+ YLR+ +YTP       EE VE    
Sbjct: 129 MAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELVEGLGM 188

Query: 185 HTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVHR 244
           HTD+S +TIV+ + + GLQMRSK G WID+ P  D  L+VNIGD M+AWSN RLRS+ HR
Sbjct: 189 HTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCND-FLVVNIGDLMQAWSNGRLRSSEHR 248

Query: 245 VVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN---- 304
           VVLRK ++ R S+AF    EDE + I AP E+VGEGK R Y+SF   +Y  FR++N    
Sbjct: 249 VVLRK-LVNRVSLAFFLCFEDE-KVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSNEEGK 308

Query: 305 YENVGATIKDFVGI 307
           +E +G T+KDF G+
Sbjct: 309 FEKIGFTVKDFAGL 316

[Close]

BLAST of CmaCh17G013400 vs. TAIR10
Match: AT5G51310.1 (AT5G51310.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 225.3 bits (573), Expect = 5.0e-59
Identity = 135/326 (41.41%), Postives = 199/326 (61.04%), Query Frame = 1


Query: 5   EVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETK 64
           E+P  DIS + L+ES+ +SL  AC E  FF++ NHG+S++ Y KL      +F   +E  
Sbjct: 4   ELPVFDIS-KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDE-- 63

Query: 65  QKLKQRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMF-------EFICSNLLDEYG 124
           +K+K   S+YTPRF+ SPF ES ++SGP F   A+  V  F       EF  S L+ EYG
Sbjct: 64  EKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEF--SGLMKEYG 123

Query: 125 SKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYT-PLGMNDEEE------ 184
            KMT++ +K++K +L   G  + +K+Y+ EF NC+ Y RIN+YT P    D+        
Sbjct: 124 EKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQD 183

Query: 185 -VEVFQKHTDLSFVTIVFPNDIRGLQMRSKGG-NWIDVRPSEDGNLIVNIGDYMEAWSNR 244
            +E    HTD+S +TIV  +DI GLQ+R++ G   +D+ P ++  L+VN+GD + AW+N 
Sbjct: 184 LIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEA-LVVNVGDLLHAWTNG 243

Query: 245 RLRSAVHRVVLRKN--IMKRFSIAFLSTPEDESREIYAPSEVVG--EGKSRLYRSFSIQQ 304
           RLRS+ HRV+L++   +  RFS+AF     D+ + ++AP EVVG  EG  R++RSF    
Sbjct: 244 RLRSSQHRVILKRRGFVGNRFSLAFFWC-FDDGKVVFAPDEVVGGCEG-MRVFRSFKCGD 303

Query: 305 YKHFRENN----YENVGATIKDFVGI 307
           Y  FRE+N    +E VG T++DF  I
Sbjct: 304 YLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of CmaCh17G013400 vs. TAIR10
Match: AT1G50960.1 (AT1G50960.1 gibberellin 2-oxidase 7)

HSP 1 Score: 103.6 bits (257), Expect = 2.2e-22
Identity = 92/312 (29.49%), Postives = 149/312 (47.76%), Query Frame = 1


Query: 5   EVPEIDISSQSLTEST-----ASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSS 64
           ++P ID+S  +  E          +  A  E  FF I NHGI K+ +  ++    +LF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 65  PEETKQKLKQRVSSYTPRFVVSPFLESFKLSGP------KFSVFARVQVCMFEFICSNLL 124
           P   K  +++R S  +          S++   P      ++SV     + + E   S + 
Sbjct: 99  PFSVK--VRERFSDLSKN--------SYRWGNPSATSPAQYSVSEAFHIILSEV--SRIS 158

Query: 125 DEYGSKMTEVSK------KVVKLLLKILG--VSIENKFYDLEFSNCNSYLRINDYTPLGM 184
           D+  +  T V        +V +++ +ILG  V++ +++++  F   NS+LR+N Y P   
Sbjct: 159 DDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVF 218

Query: 185 NDEEEVEVFQKHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAW 244
             E  V     HTD SF+TI+  + I GL++ + G  WI V+P  +  L VNIGD  +A 
Sbjct: 219 GSE--VFGLVPHTDTSFLTILSQDQIGGLELENNG-QWISVKPCLEA-LTVNIGDMFQAL 278

Query: 245 SNRRLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQY 298
           SN   +S  HRV+   NI +R SIAF   P       Y  +E+   G  + YR FS ++Y
Sbjct: 279 SNGVYQSVRHRVISPANI-ERMSIAFFVCP-------YLETEIDCFGYPKKYRRFSFREY 326

[Close]

BLAST of CmaCh17G013400 vs. TAIR10
Match: AT1G55290.1 (AT1G55290.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 99.8 bits (247), Expect = 3.2e-21
Identity = 100/313 (31.95%), Postives = 149/313 (47.60%), Query Frame = 1


Query: 2   SIEEVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPE 61
           S E +P IDIS+    +S + ++  A  E  FF + NHG+S E    + +   + F  P 
Sbjct: 58  SDESIPVIDISNLD-EKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPV 117

Query: 62  ETKQKLKQRVS-SYTPRFVVS--PFLESFKLSGPKFSVFARVQVCMFEFICSNLLDEYGS 121
           E K+K  +  S S   RF  S  P  E         S+F   +    +    +   E   
Sbjct: 118 EEKRKFSREKSLSTNVRFGTSFSPHAEKALEWKDYLSLFFVSEAEASQLWPDSCRSETLE 177

Query: 122 KMTEVSKKVVKLLLKILGVSIENKFYD-LEFSNCNSYLRIN-DYTPLGMNDEEEVEVFQK 181
            M E +K +VK LL+ LG ++  K  D  + S      RIN +Y P+  N E  V V  +
Sbjct: 178 YMNE-TKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPNPELTVGV-GR 237

Query: 182 HTDLSFVTIVFPNDIRGLQMRS-KGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVH 241
           H+D+S +TI+  ++I GL +RS   G W+ V P   G+L++NIGD M+  SN R +S  H
Sbjct: 238 HSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPIS-GSLVINIGDAMQIMSNGRYKSVEH 297

Query: 242 RVVLRKNIMKRFSIAFLSTPEDESREIYAP-SEVVGEGKSRLYRSFSIQQY-KHFRENNY 301
           R VL      R S+    +P+ ES  +  P  EV+  G+  +Y+      Y KHF    +
Sbjct: 298 R-VLANGSYNRISVPIFVSPKPES--VIGPLLEVIENGEKPVYKDILYTDYVKHFFRKAH 357

Query: 302 ENVGATIKDFVGI 307
           +  G    DF  I
Sbjct: 358 D--GKKTIDFANI 361

[Close]

BLAST of CmaCh17G013400 vs. TAIR10
Match: AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)

HSP 1 Score: 99.4 bits (246), Expect = 4.2e-21
Identity = 81/311 (26.05%), Postives = 141/311 (45.34%), Query Frame = 1


Query: 6   VPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETKQ 65
           VP ID+SSQ  T      +++ACT+  FF + NHG+S+         ++  F  P   KQ
Sbjct: 63  VPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQ 122

Query: 66  KLKQR-------VSSYTPRFVVS-PFLESFKLSGPKFSVFARVQVCMFEFICSNLLDEYG 125
           K +++        SS+T RF    P+ E+        +  +R     F        +++G
Sbjct: 123 KAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLGQEFEQFG 182

Query: 126 SKMTEVSKKVVKLLLKI-----LGVSIENKFYDLEFSNCNSYLRINDYTPLGMNDEEEVE 185
               +  + +  L LKI     L + +   ++   F   +S +R+N Y P    D     
Sbjct: 183 KVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPD--LTL 242

Query: 186 VFQKHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRS 245
               H D S +TI+  + + GLQ+      W  +RP+     +VNIGD   A SN   +S
Sbjct: 243 GTGPHCDPSSLTILHQDHVNGLQV-FVDNQWQSIRPNPKA-FVVNIGDTFMALSNGIFKS 302

Query: 246 AVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN 304
            +HR V+ +   ++ S+AF   P+ + + +  PS+++ + K+R Y  F+   +  F + +
Sbjct: 303 CLHRAVVNRESARK-SMAFFLCPK-KDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKH 362

[Close]

BLAST of CmaCh17G013400 vs. NCBI nr
Match: gi|449458351|ref|XP_004146911.1| (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus])

HSP 1 Score: 305.8 bits (782), Expect = 8.3e-80
Identity = 168/318 (52.83%), Postives = 227/318 (71.38%), Query Frame = 1


Query: 4   EEVPEIDISS-QSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSS-PE 63
           EE+  +D++S + L+ES+  SL KAC E   F+IKNHG+SKE Y KL +  D+L ++ PE
Sbjct: 10  EELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPE 69

Query: 64  ETKQ-KLKQRVSSYTPRFVVSPFLESFKLSGPKFSVFAR----------VQVCMFEFICS 123
           ETK+ KLK   S YTPRF +SP++ESFK  GP FS +A            +V  F     
Sbjct: 70  ETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFR---- 129

Query: 124 NLLDEYGSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYTPLGMNDEEE 183
            LLDEYGS M E+S++++KLLLK +G ++E+KFY+ EFSNCN YLRIN Y P   N  EE
Sbjct: 130 KLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAP--RNSNEE 189

Query: 184 VEVFQKHTDLSFVTIVFPNDIRGLQMRSK-GGNWIDVRPSEDGNLIVNIGDYMEAWSNRR 243
           +E F KHTD+S VTI+F ++I G+QM+ K G  W+DVRP ED  L+VNIGD+++AWSN R
Sbjct: 190 IEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDA-LLVNIGDFLQAWSNER 249

Query: 244 LRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFR 303
           LRSA HRVVL++++ KRFS+AF    +D+ RE+YAPSEVVGEG +R+Y+ FS ++Y+ +R
Sbjct: 250 LRSAEHRVVLKQDV-KRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYR 309

Query: 304 ENNYEN-VGATIKDFVGI 307
           ENNY   VG  +++F GI
Sbjct: 310 ENNYRKIVGVPLREFAGI 319

[Close]

BLAST of CmaCh17G013400 vs. NCBI nr
Match: gi|659107869|ref|XP_008453897.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo])

HSP 1 Score: 305.4 bits (781), Expect = 1.1e-79
Identity = 171/319 (53.61%), Postives = 226/319 (70.85%), Query Frame = 1


Query: 4   EEVPEIDISS-QSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSS-PE 63
           EE+P +D++  + L+ES+  SL KAC E   F+IKNHG+SKE Y+KL +  D+L ++ PE
Sbjct: 10  EELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAVPE 69

Query: 64  ETKQ-KLKQRVSSYTPRFVVSPFLESFKLSGPKFS-----------VFARVQVCMFEFIC 123
           E K+ KLK  VS YTPRF +SP+ ESFK  GP FS           VF   +V  F    
Sbjct: 70  EAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGH-RVTQFR--- 129

Query: 124 SNLLDEYGSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYTPLGMNDEE 183
            NLLDEYGS M E+S++++KLLLK++G S E+KFY+ EFSNCN Y+RIN Y P   N  E
Sbjct: 130 -NLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAP--RNSNE 189

Query: 184 EVEVFQKHTDLSFVTIVFPNDIRGLQMRSK-GGNWIDVRPSEDGNLIVNIGDYMEAWSNR 243
           E+E F KHTD+S VTI+F ++I G+QM+SK G  W+DVRP ED  L+VNIGD+++AWSN 
Sbjct: 190 EIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDA-LLVNIGDFLQAWSNE 249

Query: 244 RLRSAVHRVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHF 303
           RLRSA HRVVL++N  KRF++ F     D+ RE+YAPSEVVGEG  R+Y+ FS ++Y+ F
Sbjct: 250 RLRSAEHRVVLKQN-AKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 309

Query: 304 RENNY-ENVGATIKDFVGI 307
           RENNY + VG  +++F GI
Sbjct: 310 RENNYGKVVGVPMREFAGI 319

[Close]

BLAST of CmaCh17G013400 vs. NCBI nr
Match: gi|661887686|emb|CDP08817.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 272.7 bits (696), Expect = 7.8e-70
Identity = 155/315 (49.21%), Postives = 206/315 (65.40%), Query Frame = 1


Query: 5   EVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETK 64
           ++P +DIS + L   + SSL+ AC+E  FF I NHGISKE Y K++S    LFS P ETK
Sbjct: 10  KLPSLDIS-KPLDSCSLSSLTAACSEWGFFQITNHGISKELYDKILSLSKDLFSLPSETK 69

Query: 65  QKLK--QRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMF------EFICSNLLDEY 124
            KL     + +YTP F+ SPF ES ++SGP F+  A+    +       EF  S +L EY
Sbjct: 70  LKLGPFSSLKTYTPHFIASPFFESLRVSGPDFAASAQDSAEVLSAPNISEF--SEILHEY 129

Query: 125 GSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYT-PLGMNDEEEVEVFQ 184
           G KMTE+SK+V+KL+L  LG   E K+Y+ EFSNC  YLRIN+Y  P  + +EEE E   
Sbjct: 130 GRKMTEISKEVLKLVLMSLGAEFEMKYYNSEFSNCQGYLRINNYAPPESLEEEEEAEGLG 189

Query: 185 KHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVH 244
            HTD+S +TIV+ +++ GLQ++S+ G W+D+ P E G L+VNIGD ++AWSN + RS+ H
Sbjct: 190 MHTDMSCITIVYQDEVGGLQVKSREGKWMDIVPCE-GTLVVNIGDVLQAWSNDKFRSSEH 249

Query: 245 RVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN--- 304
           RVVLRK ++ RFS+AF    EDE + I AP EVVG   SR+Y+ F    Y  FRENN   
Sbjct: 250 RVVLRK-LVNRFSLAFFWCFEDE-KIILAPDEVVGRENSRIYKPFVCSDYLKFRENNEKG 309

Query: 305 -YENVGATIKDFVGI 307
            +E VG T+KDF GI
Sbjct: 310 KFEKVGFTVKDFAGI 318

[Close]

BLAST of CmaCh17G013400 vs. NCBI nr
Match: gi|590671448|ref|XP_007038334.1| (2-oxoglutarate-dependent dioxygenase [Theobroma cacao])

HSP 1 Score: 269.2 bits (687), Expect = 8.6e-69
Identity = 154/313 (49.20%), Postives = 206/313 (65.81%), Query Frame = 1


Query: 5   EVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETK 64
           E+P  D+S Q L+ S+ SSLS AC E  FF I NHG+SK+ Y KL S  + LFS P+E+K
Sbjct: 9   ELPVFDLS-QPLSPSSLSSLSLACREWGFFRITNHGVSKDLYRKLYSLSNHLFSLPDESK 68

Query: 65  QKL--KQRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMF------EFICSNLLDEY 124
            KL     + +YTP F+ SPF ES ++SGP F   A+  V +       EF  S +L EY
Sbjct: 69  LKLGPTSSLKTYTPHFIASPFFESLRVSGPDFFASAQSSVDVLFEQPNSEF--SEVLKEY 128

Query: 125 GSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYTPLGMNDEEEVEVFQK 184
           GS MT++SK++V+ +LK LG  +E K ++ EF NC+ YLRIN+YTP     EEEV+    
Sbjct: 129 GSLMTKLSKEIVEAILKSLGEELEKKSFESEFGNCHGYLRINNYTPPKSMKEEEVDGLGM 188

Query: 185 HTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVHR 244
           HTD+S VTIV+ ++I GLQ+RSK G W+D+ P ED  L+VNIGD M AWSN +L S+ HR
Sbjct: 189 HTDMSCVTIVYQDEIGGLQVRSKEGKWMDINPCED-TLVVNIGDLMHAWSNGKLSSSEHR 248

Query: 245 VVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN---- 304
           VVL K I+ RFS+AF    EDE + I+AP+EVVGE   R+Y+ F    Y  FR+++    
Sbjct: 249 VVL-KRIVNRFSLAFFWCFEDE-KVIFAPNEVVGEDNLRIYKPFVCADYLKFRQSSEKGR 308

Query: 305 YENVGATIKDFVG 306
           ++ VG T+KDF G
Sbjct: 309 FDKVGFTVKDFAG 315

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BLAST of CmaCh17G013400 vs. NCBI nr
Match: gi|1009122335|ref|XP_015877948.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Ziziphus jujuba])

HSP 1 Score: 266.2 bits (679), Expect = 7.3e-68
Identity = 152/314 (48.41%), Postives = 201/314 (64.01%), Query Frame = 1


Query: 5   EVPEIDISSQSLTESTASSLSKACTELEFFFIKNHGISKEFYYKLISGIDQLFSSPEETK 64
           E+P  DI  + L  S+ SSLS AC E  FF I NHG+SK+ Y KL S   QLF  P E+K
Sbjct: 10  ELPVFDIF-KPLNPSSLSSLSLACKEWGFFHITNHGVSKDLYNKLYSLSTQLFKLPLESK 69

Query: 65  QKL--KQRVSSYTPRFVVSPFLESFKLSGPKFSVFARVQVCMF------EFICSNLLDEY 124
            K      + +YTPRF+ SPF ESF++SGP F   A+    +       EF  S +L EY
Sbjct: 70  LKAGPSSSIKTYTPRFIASPFFESFRVSGPDFFSSAQSSALLLPNQQDSEF--SEILKEY 129

Query: 125 GSKMTEVSKKVVKLLLKILGVSIENKFYDLEFSNCNSYLRINDYT-PLGMNDEEEVEVFQ 184
           GSKMTE+SK++++++L  +G + ENKFYD EF NC+ YLRI +YT P  + +EEEVE   
Sbjct: 130 GSKMTELSKRIIEVVLMSMGENFENKFYDSEFRNCHGYLRIINYTPPANVEEEEEVEGLG 189

Query: 185 KHTDLSFVTIVFPNDIRGLQMRSKGGNWIDVRPSEDGNLIVNIGDYMEAWSNRRLRSAVH 244
            HTD+S +TIV+ +   GLQ+RSK G W+D+ P E G L+VNIGD M+AWSN +LRS+ H
Sbjct: 190 MHTDMSCITIVYQDQTGGLQVRSKEGKWMDINPCE-GTLVVNIGDLMQAWSNGKLRSSQH 249

Query: 245 RVVLRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFREN---- 304
           RVVL +  + RFS+AF    ED+ + IY P E+V E   R+Y+ F    Y  FRE+    
Sbjct: 250 RVVLTR-FVNRFSLAFFWCFEDQ-KLIYTPKEIVSEEHLRVYKPFICADYLKFRESREEG 309

Query: 305 NYENVGATIKDFVG 306
            +E VG T+KDF G
Sbjct: 310 KFEKVGLTVKDFAG 317

The following BLAST results are available for this feature:

BLAST of CmaCh17G013400 vs. Swiss-Prot


Match Name	E-value	Identity	Description
G2OX7_ARATH	3.9e-21	29.49	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1	[more]
G3OX_PEA	2.5e-20	27.60	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum GN=LE PE=1 SV=1	[more]
F6H2_ARATH	5.6e-20	31.95	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1	[more]
GAOX2_ARATH	7.4e-20	26.05	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1	[more]
F6H1_ARATH	1.3e-19	32.09	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana GN=F6'H1 PE=1 SV=1	[more]

BLAST of CmaCh17G013400 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KW97_CUCSA	5.8e-80	52.83	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G022900 PE=3 SV=1	[more]
A0A068UKJ5_COFCA	5.4e-70	49.21	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00027923001 PE=3 SV=1	[more]
A0A061FZ75_THECC	6.0e-69	49.20	2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao GN=TCM_014890 PE=3 SV=1	[more]
D7TVY9_VITVI	1.2e-66	46.50	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03440 PE=3 SV=...	[more]
A0A0A0KB92_CUCSA	1.6e-66	46.06	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G004480 PE=3 SV=1	[more]

BLAST of CmaCh17G013400 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G23340.1	6.5e-67	45.86	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G51310.1	5.0e-59	41.41	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G50960.1	2.2e-22	29.49	gibberellin 2-oxidase 7	[more]
AT1G55290.1	3.2e-21	31.95	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G51810.1	4.2e-21	26.05	gibberellin 20 oxidase 2	[more]

BLAST of CmaCh17G013400 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449458351\|ref\|XP_004146911.1\|	8.3e-80	52.83	PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus]	[more]
gi\|659107869\|ref\|XP_008453897.1\|	1.1e-79	53.61	PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo]	[more]
gi\|661887686\|emb\|CDP08817.1\|	7.8e-70	49.21	unnamed protein product [Coffea canephora]	[more]
gi\|590671448\|ref\|XP_007038334.1\|	8.6e-69	49.20	2-oxoglutarate-dependent dioxygenase [Theobroma cacao]	[more]
gi\|1009122335\|ref\|XP_015877948.1\|	7.3e-68	48.41	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR026992	DIOX_N
IPR027443	IPNS-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh17G013400.1	CmaCh17G013400.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 160..254 score: 2.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 151..255 score: 10
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 6..70 score: 1.8
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 4..293 score: 3.9
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 5..300 score: 1.8
None	No IPR available	PANTHER	PTHR10209:SF151	SUBFAMILY NOT NAMED	coord: 5..300 score: 1.8
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 2..292 score: 1.24

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh17G013400	Cucumber (Gy14) v2	cgybcmaB920
CmaCh17G013400	Melon (DHL92) v3.6.1	cmamedB374
CmaCh17G013400	Silver-seed gourd	carcmaB0020
CmaCh17G013400	Silver-seed gourd	carcmaB0104
CmaCh17G013400	Cucumber (Chinese Long) v3	cmacucB0457
CmaCh17G013400	Watermelon (97103) v2	cmawmbB390
CmaCh17G013400	Wax gourd	cmawgoB0493
CmaCh17G013400	Cucurbita maxima (Rimu)	cmacmaB374
CmaCh17G013400	Cucumber (Gy14) v1	cgycmaB1077
CmaCh17G013400	Cucurbita moschata (Rifu)	cmacmoB353
CmaCh17G013400	Cucurbita moschata (Rifu)	cmacmoB366
CmaCh17G013400	Wild cucumber (PI 183967)	cmacpiB388
CmaCh17G013400	Cucumber (Chinese Long) v2	cmacuB384
CmaCh17G013400	Melon (DHL92) v3.5.1	cmameB322
CmaCh17G013400	Watermelon (Charleston Gray)	cmawcgB309
CmaCh17G013400	Watermelon (97103) v1	cmawmB331
CmaCh17G013400	Cucurbita pepo (Zucchini)	cmacpeB371
CmaCh17G013400	Cucurbita pepo (Zucchini)	cmacpeB392
CmaCh17G013400	Bottle gourd (USVL1VR-Ls)	cmalsiB346