CmaCh17G006480 (gene) Cucurbita maxima (Rimu)

NameCmaCh17G006480
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPectinesterase
LocationCma_Chr17 : 5472057 .. 5474964 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTTTGTCTGCGGGTCTATATTAGCCCTTATTCTCTTCTCCACTATCTCACACCACCTCCCACAAATCCCCTCTCTTATTCCGACAACATGGAACAACCCATCGCCGCCGGAATCTCAAGTTCCGGCAACCACCTCCCAAAGCCGAAATACAACAGAAAAAAGCTCATAATCTTAGCCGTGTCTTCAACCCTCCTTCTGGTGGGCATCGTCGCCGGAGTGGTGAGCTCGAGAAACGCCGGCGGTGAGGAGTCCAAAAGCCATGCCATTGTGAAGAGCACGTGCAGTAGCACGCTGTATCCGGATCTGTGCTTTTCGAGCATTTTGAAGAACGATGAGGCGATGAAGAAAGTTAGTAATAAAAAGGATGTGATTGAGGTGTCGATTAATATTACGGTTAAAGCGGTGGAGGAGAATTATTTTAAGGTTTTGAAATTGTATGAGTTGAAGAATATTAGTCATAGGGAGAAGATTGCTCTTCATGATTGCCTTGAAACTATTGATGAGACGCTTGATGAGCTTCATAAGGCTACTGTTGACCTCCATGAATACCCAAATAAGAAGTCTCTTACTCAACAGGCTGATGATCTCAAGACCTTGCTTAGTTCCGCCATTACTAATCAGGTATTGAATTACGACTCTCTATAATAGTATGATATTGTCCGCTCTAAGCTTATGTTCCGTGTGTTTTACTTCAGTCACTTGTTTACTACATCTTCGAGGTTCACAACTTTTTTGTTCGATATTTGAGAAATCTAGTTAAGGGCATTACTCTGATGACATGTTAGTTTGACATTTGAAGATTCTAGTTAAGGGCACAACTCTAATACTATATTAGAAATTTAGGCTCTCCATGATAGAATGTTTGTCCACTCTAACCATAGACTCTCTTGATTTTATTTTTTTGTTTCGTAAAAGGTTTCAGGTATATTTTACTTCATTAACCAATGTGTGACTCCCTTACTCTCAAGACGTGTGAACGCTCATCTTAAATAAATTACAATAAATTTAATCTTTTATTCGTATTTCCTCCGACAGGAGACATGCCTCGACGGGTTTTCCCACGACGAGGCGGACAAGAACCTGCGGGACAAGCTAAAAGAGGGTCAAATAGAGGTAGAGCGCATGTGCAGCAACGCGCTAGCCATGATTAAGAACATGACCGATACAGACATAGCCAACTACGAGACCAAAATGGGAACAGCAGCCAACAGAAAGCTCAAGGAAGAGAACAACGACGAGCAAATCCAGTGGCCGGAGTGGCTGTCAGCCGGAGACCGACGTCTGCTGCAATCATCCTCCGTGAAGCCGGACGTGGTCGTGGCGGCGGATGGCAGCGGGAACTTCAAGACAGTGGCGGCCGCCGTGGCCGCCGCCCCGGTAAAAAGTAGCAAGAGATATGTGATCAGAATTAAAGCTGGGGTTTATAGAGAGAATGTGGATGTGCAAAAGAAGAAGACGAATATAATGTTTATGGGAGATGGAAGAAGTAACACTATAATTACCGGTAGCCGGAATGTCGTCGACGGCAGCACCACTTTTAACTCTGCCACCGTCGGTAAGCTAAATTTTTACCCGTTTTTTCAGTAATTTTTTTAATTTTTTTTTACCTATTTTTTAAATTTGTCAAACTAGTCTTAATTACAAATTTAATCGATCTCAAATTGTAATTATTATTTTAATTAATTACCCATAAAAGTACAATTTTGACCCTCTCCACTCGATAAGCTTTTCAAAGCTTATCATTCCCCGTTGAAAGATACCTTCCATCCAATGCATGGTGGAGCGCACATGGGCAAAAGTGTCCGATATCCATAAAAATAAAAAATAAATAATTTTTTTTATTTTTTTATTTTTATCCAGCACTGAATCAGAATCTATATTTATTTTTTAATCCAAATATAATTTTGTTGATTTTATTAGATATTATCCTTTCAATTCAAATGTCAATTTTAAAAAATATATTTCATTTTATAAAAAGAAAAATAATATTCTCCTTTGATTTGAACTGATGCCGACACGTGGATCATGATTTTGACAGCTGCGGTGGGAGAAGGATTTCTAGCGCGTGACATCACATTCCAAAACACAGCCGGTCCCTCAAAGCACCAAGCGGTGGCTCTCCGCGTGGGAGCAGATCTCTCCGCCTTCTACCAATGCGACATGTTGGCTTATCAAGACACTCTCTACGTCCACTCCAACCGTCAGTTCTACATCAACTGTCTCATTTCCGGCACCGTCGATTTCATCTTCGGAAACGCCGCTGCCGTTTTCCAAGACTGTGACATTCACGCGCGTAGACCAAACTCGGGTCAGAAAAACATGGTCACGGCTCAGGGTCGGTCCGACCCAAACCAGAACACCGGAATCGTTATCCAGAAATCCCGAATCGGAGCCACCTCCGATCTCCGGTCGGTCCAGAAGAGCTTCCCGACGTTCCTCGGAAGACCGTGGAAGGAATATTCTAGAACCGTGATCATGCAGTCGTCGATAAGTGACGTCATTGACTCGAAGGGATGGCATGAGTGGAGCGGTAATTTTGCTCTCAACACGTTGTTTTACGGTGAGTATCAGAATACTGGACCGGGGGCTTCGACGGGCGGGAGGGTGACGTGGAAGGGGTTCAGGGTAATTCGGAGTGCCACTGAGGCTGAAGGTTTTACTCCTGGTAAATTTATTGGCGGTGGGAGTTGGTTGCGGGCGACTGGGTTTCCTTTTTCTCTAGGACTATGATAACAATAATAAGCTGTAATTTTATTTTTTACCACCTATTTACTGTGGTAATTATTTTTCTATTTTCTTTTTTTAATGTGTCATTATTATGTGAGCGTGTAAGTTTGTTAAAATAAATACACATTTTCTATTTTCCTTTTAGTCTCGTGCATTTATCTATTTATTGACTTTTTTTA

mRNA sequence

CCCTTTGTCTGCGGGTCTATATTAGCCCTTATTCTCTTCTCCACTATCTCACACCACCTCCCACAAATCCCCTCTCTTATTCCGACAACATGGAACAACCCATCGCCGCCGGAATCTCAAGTTCCGGCAACCACCTCCCAAAGCCGAAATACAACAGAAAAAAGCTCATAATCTTAGCCGTGTCTTCAACCCTCCTTCTGGTGGGCATCGTCGCCGGAGTGGTGAGCTCGAGAAACGCCGGCGGTGAGGAGTCCAAAAGCCATGCCATTGTGAAGAGCACGTGCAGTAGCACGCTGTATCCGGATCTGTGCTTTTCGAGCATTTTGAAGAACGATGAGGCGATGAAGAAAGTTAGTAATAAAAAGGATGTGATTGAGGTGTCGATTAATATTACGGTTAAAGCGGTGGAGGAGAATTATTTTAAGGTTTTGAAATTGTATGAGTTGAAGAATATTAGTCATAGGGAGAAGATTGCTCTTCATGATTGCCTTGAAACTATTGATGAGACGCTTGATGAGCTTCATAAGGCTACTGTTGACCTCCATGAATACCCAAATAAGAAGTCTCTTACTCAACAGGCTGATGATCTCAAGACCTTGCTTAGTTCCGCCATTACTAATCAGGAGACATGCCTCGACGGGTTTTCCCACGACGAGGCGGACAAGAACCTGCGGGACAAGCTAAAAGAGGGTCAAATAGAGGTAGAGCGCATGTGCAGCAACGCGCTAGCCATGATTAAGAACATGACCGATACAGACATAGCCAACTACGAGACCAAAATGGGAACAGCAGCCAACAGAAAGCTCAAGGAAGAGAACAACGACGAGCAAATCCAGTGGCCGGAGTGGCTGTCAGCCGGAGACCGACGTCTGCTGCAATCATCCTCCGTGAAGCCGGACGTGGTCGTGGCGGCGGATGGCAGCGGGAACTTCAAGACAGTGGCGGCCGCCGTGGCCGCCGCCCCGGTAAAAAGTAGCAAGAGATATGTGATCAGAATTAAAGCTGGGGTTTATAGAGAGAATGTGGATGTGCAAAAGAAGAAGACGAATATAATGTTTATGGGAGATGGAAGAAGTAACACTATAATTACCGGTAGCCGGAATGTCGTCGACGGCAGCACCACTTTTAACTCTGCCACCGTCGCTGCGGTGGGAGAAGGATTTCTAGCGCGTGACATCACATTCCAAAACACAGCCGGTCCCTCAAAGCACCAAGCGGTGGCTCTCCGCGTGGGAGCAGATCTCTCCGCCTTCTACCAATGCGACATGTTGGCTTATCAAGACACTCTCTACGTCCACTCCAACCGTCAGTTCTACATCAACTGTCTCATTTCCGGCACCGTCGATTTCATCTTCGGAAACGCCGCTGCCGTTTTCCAAGACTGTGACATTCACGCGCGTAGACCAAACTCGGGTCAGAAAAACATGGTCACGGCTCAGGGTCGGTCCGACCCAAACCAGAACACCGGAATCGTTATCCAGAAATCCCGAATCGGAGCCACCTCCGATCTCCGGTCGGTCCAGAAGAGCTTCCCGACGTTCCTCGGAAGACCGTGGAAGGAATATTCTAGAACCGTGATCATGCAGTCGTCGATAAGTGACGTCATTGACTCGAAGGGATGGCATGAGTGGAGCGGTAATTTTGCTCTCAACACGTTGTTTTACGGTGAGTATCAGAATACTGGACCGGGGGCTTCGACGGGCGGGAGGGTGACGTGGAAGGGGTTCAGGGTAATTCGGAGTGCCACTGAGGCTGAAGGTTTTACTCCTGGTAAATTTATTGGCGGTGGGAGTTGGTTGCGGGCGACTGGGTTTCCTTTTTCTCTAGGACTATGATAACAATAATAAGCTGTAATTTTATTTTTTACCACCTATTTACTGTGGTAATTATTTTTCTATTTTCTTTTTTTAATGTGTCATTATTATGTGAGCGTGTAAGTTTGTTAAAATAAATACACATTTTCTATTTTCCTTTTAGTCTCGTGCATTTATCTATTTATTGACTTTTTTTA

Coding sequence (CDS)

ATGGAACAACCCATCGCCGCCGGAATCTCAAGTTCCGGCAACCACCTCCCAAAGCCGAAATACAACAGAAAAAAGCTCATAATCTTAGCCGTGTCTTCAACCCTCCTTCTGGTGGGCATCGTCGCCGGAGTGGTGAGCTCGAGAAACGCCGGCGGTGAGGAGTCCAAAAGCCATGCCATTGTGAAGAGCACGTGCAGTAGCACGCTGTATCCGGATCTGTGCTTTTCGAGCATTTTGAAGAACGATGAGGCGATGAAGAAAGTTAGTAATAAAAAGGATGTGATTGAGGTGTCGATTAATATTACGGTTAAAGCGGTGGAGGAGAATTATTTTAAGGTTTTGAAATTGTATGAGTTGAAGAATATTAGTCATAGGGAGAAGATTGCTCTTCATGATTGCCTTGAAACTATTGATGAGACGCTTGATGAGCTTCATAAGGCTACTGTTGACCTCCATGAATACCCAAATAAGAAGTCTCTTACTCAACAGGCTGATGATCTCAAGACCTTGCTTAGTTCCGCCATTACTAATCAGGAGACATGCCTCGACGGGTTTTCCCACGACGAGGCGGACAAGAACCTGCGGGACAAGCTAAAAGAGGGTCAAATAGAGGTAGAGCGCATGTGCAGCAACGCGCTAGCCATGATTAAGAACATGACCGATACAGACATAGCCAACTACGAGACCAAAATGGGAACAGCAGCCAACAGAAAGCTCAAGGAAGAGAACAACGACGAGCAAATCCAGTGGCCGGAGTGGCTGTCAGCCGGAGACCGACGTCTGCTGCAATCATCCTCCGTGAAGCCGGACGTGGTCGTGGCGGCGGATGGCAGCGGGAACTTCAAGACAGTGGCGGCCGCCGTGGCCGCCGCCCCGGTAAAAAGTAGCAAGAGATATGTGATCAGAATTAAAGCTGGGGTTTATAGAGAGAATGTGGATGTGCAAAAGAAGAAGACGAATATAATGTTTATGGGAGATGGAAGAAGTAACACTATAATTACCGGTAGCCGGAATGTCGTCGACGGCAGCACCACTTTTAACTCTGCCACCGTCGCTGCGGTGGGAGAAGGATTTCTAGCGCGTGACATCACATTCCAAAACACAGCCGGTCCCTCAAAGCACCAAGCGGTGGCTCTCCGCGTGGGAGCAGATCTCTCCGCCTTCTACCAATGCGACATGTTGGCTTATCAAGACACTCTCTACGTCCACTCCAACCGTCAGTTCTACATCAACTGTCTCATTTCCGGCACCGTCGATTTCATCTTCGGAAACGCCGCTGCCGTTTTCCAAGACTGTGACATTCACGCGCGTAGACCAAACTCGGGTCAGAAAAACATGGTCACGGCTCAGGGTCGGTCCGACCCAAACCAGAACACCGGAATCGTTATCCAGAAATCCCGAATCGGAGCCACCTCCGATCTCCGGTCGGTCCAGAAGAGCTTCCCGACGTTCCTCGGAAGACCGTGGAAGGAATATTCTAGAACCGTGATCATGCAGTCGTCGATAAGTGACGTCATTGACTCGAAGGGATGGCATGAGTGGAGCGGTAATTTTGCTCTCAACACGTTGTTTTACGGTGAGTATCAGAATACTGGACCGGGGGCTTCGACGGGCGGGAGGGTGACGTGGAAGGGGTTCAGGGTAATTCGGAGTGCCACTGAGGCTGAAGGTTTTACTCCTGGTAAATTTATTGGCGGTGGGAGTTGGTTGCGGGCGACTGGGTTTCCTTTTTCTCTAGGACTATGA

Protein sequence

MEQPIAAGISSSGNHLPKPKYNRKKLIILAVSSTLLLVGIVAGVVSSRNAGGEESKSHAIVKSTCSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEENNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRIKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL
BLAST of CmaCh17G006480 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 2.8e-238
Identity = 428/573 (74.69%), Postives = 486/573 (84.82%), Query Frame = 1

Query: 14  NHLPKPKYNRKKLIILAVSSTLLLV----GIVAGVVSSRNAGGEESKSH-AIVKSTCSST 73
           +++PK    +KK + LA+ +TLL+V    GIVAGV S +N+G   ++ H AI+KS+CSST
Sbjct: 21  SNIPK----KKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSST 80

Query: 74  LYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNISHREKI 133
            YPDLCFS+I    EA KKV+++KDVIE+S+NIT  AVE NYF + KL +  N++ REK+
Sbjct: 81  RYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKV 140

Query: 134 ALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGFSHD 193
           ALHDCLETIDETLDELHKA  DL EYPNKKSL+Q ADDLKTL+S+A+TNQ TCLDGFSHD
Sbjct: 141 ALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHD 200

Query: 194 EADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEENNDEQI 253
           +A+K++RD L +GQ+ VE+MCSNALAMIKNMTDTD+      M T+ NRKL EE +    
Sbjct: 201 DANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMI----MRTSNNRKLIEETSTVD- 260

Query: 254 QWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRIKAGVY 313
            WP WLS GDRRLLQSSSV P+VVVAADGSGNFKTVAA+VAAAP   +KRY+IRIKAGVY
Sbjct: 261 GWPAWLSTGDRRLLQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVY 320

Query: 314 RENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNT 373
           RENV+V KK  NIMF+GDGR+ TIITGSRNVVDGSTTF SATVA VGEGFLARDITFQNT
Sbjct: 321 RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNT 380

Query: 374 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAAV 433
           AGPSKHQAVALRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCLI+GTVDFIFGNAAAV
Sbjct: 381 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 440

Query: 434 FQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPTFLGRP 493
            Q+CDIHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+ VQ SFPT+LGRP
Sbjct: 441 LQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 500

Query: 494 WKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGFRV 553
           WKEYSRTVIMQSSI+DVI   GWHEW GNFALNTLFYGE+QN G GA T GRV WKGFRV
Sbjct: 501 WKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRV 560

Query: 554 IRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           I SATEA+ FTPG FI G SWL +TGFPFSLGL
Sbjct: 561 ITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CmaCh17G006480 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 1.1e-237
Identity = 429/577 (74.35%), Postives = 486/577 (84.23%), Query Frame = 1

Query: 10  SSSGNHLPKPKYNRKKLIILAVSSTLLLV----GIVAGVVSSRNAGGEESKSH-AIVKST 69
           SS+ N    PK  +KK + LA+ +TLL+V    GIVAGV S +N+G   ++ H AI+KS+
Sbjct: 15  SSNQNISNIPK--KKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSS 74

Query: 70  CSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNISH 129
           CSST YPDLCFS+I    EA KKV+++KDVIE+S+NIT  AVE NYF + KL +  N++ 
Sbjct: 75  CSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTK 134

Query: 130 REKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDG 189
           REK+ALHDCLETIDETLDELHKA  DL EYPNKKSL+Q ADDLKTL+S+A+TNQ TCLDG
Sbjct: 135 REKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDG 194

Query: 190 FSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEENN 249
           FSHD+A+K++RD L +GQ+ VE+MCSNALAMIKNMTDTD+      M T+ NRKL EE +
Sbjct: 195 FSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMI----MRTSNNRKLTEETS 254

Query: 250 DEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRIK 309
                WP WLS GDRRLLQSSSV P+ VVAADGSGNFKTVAAAVAAAP   +KRY+IRIK
Sbjct: 255 TVD-GWPAWLSPGDRRLLQSSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIK 314

Query: 310 AGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDIT 369
           AGVYRENV+V KK  NIMF+GDGR+ TIITGSRNVVDGSTTF SAT A VGEGFLARDIT
Sbjct: 315 AGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDIT 374

Query: 370 FQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGN 429
           FQNTAGPSKHQAVALRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCLI+GTVDFIFGN
Sbjct: 375 FQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGN 434

Query: 430 AAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPTF 489
           AAAV Q+CDIHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+ VQ SFPT+
Sbjct: 435 AAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTY 494

Query: 490 LGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWK 549
           LGRPWKEYSRTVIMQSSI+D+I   GWHEW GNFALNTLFYGE+QN+G GA T GRV WK
Sbjct: 495 LGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWK 554

Query: 550 GFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           GFRVI SATEA+ FTPG FI G SWL +TGFPFSLGL
Sbjct: 555 GFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CmaCh17G006480 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 9.1e-229
Identity = 415/571 (72.68%), Postives = 473/571 (82.84%), Query Frame = 1

Query: 23  RKKLIILAVSSTLLLV----GIVAGVVS-SRNAGGE------ESKSHAIVKSTCSSTLYP 82
           RKK I LA+ +++LLV    G+VAGV S S+N+          S +HAIVKS CS+TL+P
Sbjct: 17  RKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHP 76

Query: 83  DLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLYEL-KNISHREKIAL 142
           +LC+S+I+   +  KKV+++KDVIE+S+NITVKAV  NY+ V +L +  K ++ REK+AL
Sbjct: 77  ELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVAL 136

Query: 143 HDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGFSHDEA 202
           HDCLET+DETLDELH A  DL  YPNKKSL +  +DLKTL+SSAITNQETCLDGFSHDEA
Sbjct: 137 HDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDEA 196

Query: 203 DKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEENNDEQIQW 262
           DK +R  L +GQ  VE+MCSNALAMI NMTDTDIAN       A NRKL E+N     +W
Sbjct: 197 DKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLVEDNG----EW 256

Query: 263 PEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRIKAGVYRE 322
           PEWLSAGDRRLLQSS+V PDVVVAADGSG++KTV+ AV  AP KSSKRYVIRIKAGVYRE
Sbjct: 257 PEWLSAGDRRLLQSSTVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVYRE 316

Query: 323 NVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNTAG 382
           NVDV KKKTNIMFMGDG+SNTIIT SRNV DGSTTF+SATV  V    LARDITFQNTAG
Sbjct: 317 NVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNTAG 376

Query: 383 PSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAAVFQ 442
            SKHQAVAL VG+DLSAFY+CDMLAYQDTLYVHSNRQF++ CL++GTVDFIFGN AAVFQ
Sbjct: 377 ASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVFQ 436

Query: 443 DCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPTFLGRPWK 502
           DCDIHARRP SGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDLR VQKSFPT+LGRPWK
Sbjct: 437 DCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWK 496

Query: 503 EYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGFRVIR 562
           EYSRTVIMQSSI+DVI   GWHEW+GNFAL+TLFYGEY NTG GA T GRV WKG +VI 
Sbjct: 497 EYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKVIT 556

Query: 563 SATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           S+TEA+ +TPG+FI GGSWL +TGFPFSLGL
Sbjct: 557 SSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of CmaCh17G006480 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 776.9 bits (2005), Expect = 1.5e-223
Identity = 403/578 (69.72%), Postives = 464/578 (80.28%), Query Frame = 1

Query: 23  RKKLIILAVSSTLLLVGIVAGVVSSRNAGGEE----SKSHAIVKSTCSSTLYPDLCFSSI 82
            KKL++L+ +  LL V  VAG+ +  +   E+      SHA+++S+CSST YP+LC S++
Sbjct: 17  NKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAV 76

Query: 83  LKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLY-ELKNISHREKIALHDCLETI 142
           +       +++++KDVIE S+N+T+ AVE NYF V KL  + K ++ REK ALHDCLETI
Sbjct: 77  VTAGGV--ELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 136

Query: 143 DETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGFSHDEADKNLRDK 202
           DETLDELH+   DLH YP KK+L + A DLKTL+SSAITNQETCLDGFSHD+ADK +R  
Sbjct: 137 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 196

Query: 203 LKEGQIEVERMCSNALAMIKNMTDTDIANYETKMG-TAANRKLKEENNDEQIQ------- 262
           L +GQI VE MCSNALAMIKNMTDTDIAN+E K   T+ NRKLKEEN +  +        
Sbjct: 197 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAG 256

Query: 263 ------WPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRI 322
                 WP WLSAGDRRLLQ S VK D  VAADGSG FKTVAAAVAAAP  S+KRYVI I
Sbjct: 257 ELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVIHI 316

Query: 323 KAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDI 382
           KAGVYRENV+V KKK NIMFMGDGR+ TIITGSRNVVDGSTTF+SATVAAVGE FLARDI
Sbjct: 317 KAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDI 376

Query: 383 TFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFG 442
           TFQNTAGPSKHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF++ CLI+GTVDFIFG
Sbjct: 377 TFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFG 436

Query: 443 NAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPT 502
           NAA V QDCDIHARRPNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+SV+ SFPT
Sbjct: 437 NAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPT 496

Query: 503 FLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTW 562
           +LGRPWKEYS+TVIMQS+ISDVI  +GW EW+G FALNTL Y EY NTG GA T  RV W
Sbjct: 497 YLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKW 556

Query: 563 KGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           +GF+VI +A EA+ +T G+FIGGG WL +TGFPFSLGL
Sbjct: 557 RGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CmaCh17G006480 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 756.1 bits (1951), Expect = 2.8e-217
Identity = 393/574 (68.47%), Postives = 460/574 (80.14%), Query Frame = 1

Query: 22  NRKKLIILAVSSTLLLVGIVAGVVSSRNAGGEESK-------SHAIVKSTCSSTLYPDLC 81
           N KKLI+ + +  LLL+  + G+ ++     +  K       SHAI+KS CSSTLYP+LC
Sbjct: 16  NNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELC 75

Query: 82  FSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLY-ELKNISHREKIALHDC 141
           FS++       K+++++K+VIE S+N+T KAV+ NYF V KL  + K ++ RE  ALHDC
Sbjct: 76  FSAVAAT--GGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 135

Query: 142 LETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGFSHDEADKN 201
           LETIDETLDELH A  DLH+YP +KSL + ADDLKTL+SSAITNQ TCLDGFS+D+AD+ 
Sbjct: 136 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 195

Query: 202 LRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAA-----NRKLKEENND-EQ 261
           +R  L +GQ+ VE MCSNALAMIKNMT+TDIAN+E +  ++      NRKLKE   D + 
Sbjct: 196 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDS 255

Query: 262 IQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRIKAGV 321
             WP+WLS GDRRLLQ S++K D  VA DGSG+F TVAAAVAAAP KS+KR+VI IKAGV
Sbjct: 256 DGWPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKAGV 315

Query: 322 YRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQN 381
           YRENV+V KKKTNIMF+GDGR  TIITGSRNVVDGSTTF+SATVAAVGE FLARDITFQN
Sbjct: 316 YRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQN 375

Query: 382 TAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAA 441
           TAGPSKHQAVALRVG+D SAFYQCDM AYQDTLYVHSNRQF++ C I+GTVDFIFGNAAA
Sbjct: 376 TAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAAA 435

Query: 442 VFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPTFLGR 501
           V QDCDI+ARRPNSGQKNMVTAQGRSDPNQNTGIVIQ  RIG TSDL +V+ +FPT+LGR
Sbjct: 436 VLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGR 495

Query: 502 PWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGFR 561
           PWKEYSRTVIMQS ISDVI  +GWHEWSG+FAL+TL Y EY N G GA T  RV WKG++
Sbjct: 496 PWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYK 555

Query: 562 VIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           VI S TEA+ FT G+FIGGG WL +TGFPFSL L
Sbjct: 556 VITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CmaCh17G006480 vs. TrEMBL
Match: A0A0A0KAM6_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_7G447990 PE=4 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 6.7e-279
Identity = 495/589 (84.04%), Postives = 537/589 (91.17%), Query Frame = 1

Query: 1   MEQPIAAG--ISSSGNHLPKPKYNRKKLIILAVSSTLLLVGIVAGVVS--SRNAGGEESK 60
           MEQPIAAG  +S+S +     K ++KKL+I+ + STLL+VG V G+V+  ++++     +
Sbjct: 7   MEQPIAAGAGVSNSSDLQKTQKSSKKKLLIVTLCSTLLIVGAVIGIVAGTTKSSSKYSGE 66

Query: 61  SHAIVKSTCSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKL 120
           SHAIVKSTCSSTLYPD+C+S+I +++ AM KV N+KDVIE+SINITVKAVEENYF+V KL
Sbjct: 67  SHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKL 126

Query: 121 YELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAIT 180
             LKNISHRE+IALHDCLETIDETLDELHKA VDL+EYPNKKSL Q ADDLKTLLSSAIT
Sbjct: 127 CGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAIT 186

Query: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAAN 240
           NQETCLDGFSHD+ADKNLRDKLKEGQ+EVE MCSNALAMIKNMTDTDIANYE KMG   N
Sbjct: 187 NQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKN 246

Query: 241 RKLKEE----NNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAP 300
           RKL  E    NND+ IQWPEWLSAGDRRLLQSSSV P+VVVAADGSGNF+TVAAAVAAAP
Sbjct: 247 RKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAP 306

Query: 301 VKSSKRYVIRIKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVA 360
           V+SSKRYVIRIKAGVYRENV+V KKKTNIMF+GDGR NTIITGSRNVVDGSTTFNSAT+A
Sbjct: 307 VRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMA 366

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 367 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 426

Query: 421 LISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           L+SGTVDFIFGNAAA+FQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 427 LVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 486

Query: 481 DLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTG 540
           DLR VQKSFPTFLGRPWKEYSRTVIMQ +ISDVID KGWHEWSG+FALNTLFYGEYQNTG
Sbjct: 487 DLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTG 546

Query: 541 PGASTGGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
            GASTGGRVTWKGFRVIRSATEAE FT GKFIGGGSWL +TGFPFSLGL
Sbjct: 547 AGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595

BLAST of CmaCh17G006480 vs. TrEMBL
Match: A0A061GE06_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_029251 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 3.6e-240
Identity = 433/578 (74.91%), Postives = 489/578 (84.60%), Query Frame = 1

Query: 9   ISSSGNHLPKPKYNRKKLIILAVSSTLLLV----GIVAGVVSSRNAGGEESKSHAIVKST 68
           IS SG H+   K ++K  +ILAV  +L++V    GIVAGV S  N+   ++  HAIVKS 
Sbjct: 11  ISDSGKHISSTKKHKK--VILAVFGSLVIVAAIIGIVAGVNSRNNSDETDTSHHAIVKSA 70

Query: 69  CSSTLYPDLCFSSILKNDEAM-KKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNIS 128
           CS T YPDLCFS +     A  KKV +KKDVIE+S+NIT  AVE NYFK+ KL   K ++
Sbjct: 71  CSITRYPDLCFSEVAAAPAATSKKVKSKKDVIELSLNITTTAVEHNYFKIKKLLAKKGLT 130

Query: 129 HREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLD 188
            REK ALHDCLETIDETLDELH+A  DLHEYPNKKSLTQ ADDLKTL+S+A+TNQETCLD
Sbjct: 131 EREKTALHDCLETIDETLDELHEAVEDLHEYPNKKSLTQHADDLKTLMSAAMTNQETCLD 190

Query: 189 GFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEEN 248
           GFSHD ADK +R  L +G+  VERMCSNALAMIKNMTDTDIAN   K  T++NRKLKE+ 
Sbjct: 191 GFSHDAADKKIRKVLIDGEKYVERMCSNALAMIKNMTDTDIANEMLK--TSSNRKLKEQE 250

Query: 249 NDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRI 308
           N   I WPEWLSAGDRRLLQSSSV PDVVVAADGSGN+KTV+ AVA AP +SSKRYVI+I
Sbjct: 251 NG--IAWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNYKTVSEAVAKAPQRSSKRYVIKI 310

Query: 309 KAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDI 368
           KAGVYRENV+V KKKTNIMF+GDGR+ TIITGSRNVVDGSTTF+SATVA VGE FLARDI
Sbjct: 311 KAGVYRENVEVPKKKTNIMFLGDGRTETIITGSRNVVDGSTTFHSATVAVVGERFLARDI 370

Query: 369 TFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFG 428
           TFQNTAGPSKHQAVALRVGADLSAFY+CDMLAYQDTLY HSNRQFY+NC+I+GTVDFIFG
Sbjct: 371 TFQNTAGPSKHQAVALRVGADLSAFYECDMLAYQDTLYAHSNRQFYVNCIIAGTVDFIFG 430

Query: 429 NAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPT 488
           NAAAVFQ+CDIHARRPNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+SV+ +FPT
Sbjct: 431 NAAAVFQNCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVRSNFPT 490

Query: 489 FLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTW 548
           +LGRPWKEYSRTV+MQS ISDVI   GWHEWS +FAL TLFYGEYQNTG GA T GRV W
Sbjct: 491 YLGRPWKEYSRTVVMQSVISDVIHPAGWHEWSESFALRTLFYGEYQNTGAGAGTSGRVKW 550

Query: 549 KGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           +G++VI SA+EA+ ++PG+FI GGSWL +TGFPFSLGL
Sbjct: 551 EGYKVITSASEAQAYSPGRFIAGGSWLSSTGFPFSLGL 582

BLAST of CmaCh17G006480 vs. TrEMBL
Match: J7HYD0_THECC (Pectinesterase OS=Theobroma cacao GN=PME PE=2 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 8.9e-239
Identity = 431/577 (74.70%), Postives = 486/577 (84.23%), Query Frame = 1

Query: 9   ISSSGNHLPKPKYNRKKLIILAVSSTLLLV----GIVAGVVSSRNAGGEESKSHAIVKST 68
           IS SG H+   K ++K  +ILAV  +L++V    GIVAGV S  N+   ++  HAIVKS 
Sbjct: 11  ISDSGKHISSTKKHKK--VILAVFGSLVIVAAIIGIVAGVNSRNNSDETDTSHHAIVKSA 70

Query: 69  CSSTLYPDLCFSSILKNDEAM-KKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNIS 128
           CS T YPDLCFS +     A  KKV +KKDVIE+S+NIT  AVE NYFK+ KL   K ++
Sbjct: 71  CSITRYPDLCFSEVAAAPAATSKKVKSKKDVIELSLNITTTAVEHNYFKIKKLLAKKGLT 130

Query: 129 HREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLD 188
            REK ALHDCLETIDETLDELH+A  DLHEYPNKKSLTQ ADDLKTL+S+A+TNQETCLD
Sbjct: 131 EREKTALHDCLETIDETLDELHEAVEDLHEYPNKKSLTQHADDLKTLMSAAMTNQETCLD 190

Query: 189 GFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEEN 248
           GFSHD ADK +R  L +G+  VERMCSNALAMIKNMTDTDIAN   K  T++NRKLKE+ 
Sbjct: 191 GFSHDAADKKIRKVLIDGEKYVERMCSNALAMIKNMTDTDIANEMLK--TSSNRKLKEQE 250

Query: 249 NDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRI 308
           N   I WPEWLSAGDRRLLQSSSV PDVVVAADGSGN+KTV+ AVA AP +SSKRYVI+I
Sbjct: 251 NG--IAWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNYKTVSEAVAKAPQRSSKRYVIKI 310

Query: 309 KAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDI 368
           KAGVYRENV+V KKKTNIMF+GDGR+ TIITGSRNVVDGSTTF+SATVA VGE FLAR I
Sbjct: 311 KAGVYRENVEVPKKKTNIMFLGDGRTETIITGSRNVVDGSTTFHSATVAVVGERFLARSI 370

Query: 369 TFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFG 428
           TFQNTAGPSKHQAVALRVGADLSAFY+CDMLAYQDTLY HSNRQFY+NC+I+GTVDFIFG
Sbjct: 371 TFQNTAGPSKHQAVALRVGADLSAFYECDMLAYQDTLYAHSNRQFYVNCIIAGTVDFIFG 430

Query: 429 NAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPT 488
           NAAAVFQ+CDIHARRPNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+SV  +FPT
Sbjct: 431 NAAAVFQNCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVTSNFPT 490

Query: 489 FLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTW 548
           +LGRPWKEYSRTV+MQS ISDVI   GWHEWS +FAL TLFYGEYQNTG GA T GRV W
Sbjct: 491 YLGRPWKEYSRTVVMQSVISDVIHPAGWHEWSESFALRTLFYGEYQNTGAGAGTSGRVKW 550

Query: 549 KGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLG 581
           +G++VI SA+EA+ ++PG+FI GGSWL +TGFPFSLG
Sbjct: 551 EGYKVITSASEAQAYSPGRFIAGGSWLSSTGFPFSLG 581

BLAST of CmaCh17G006480 vs. TrEMBL
Match: F6HXK8_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_09s0002g00320 PE=4 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 2.9e-237
Identity = 430/579 (74.27%), Postives = 492/579 (84.97%), Query Frame = 1

Query: 8   GISSSGNHLPKPKYNRKKLIILAVSSTLLL---VGIVAGVVSSRNAGGEE--SKSHAIVK 67
           G+S SG H    K N KKL +L  SS LL+   VGIVAGV S +++  +   +  HA++K
Sbjct: 40  GMSDSGKHTSISKRN-KKLFLLVFSSVLLIAAVVGIVAGVHSRKSSTNDVGLTAGHAVLK 99

Query: 68  STCSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNI 127
           S CSST YPDLC+S+I     A KKV+++KDVI VS+NITV AVE NYF + KL + KN+
Sbjct: 100 SACSSTRYPDLCYSAIATVPGASKKVTSQKDVIAVSLNITVTAVEHNYFTIEKLLDFKNL 159

Query: 128 SHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCL 187
           + REK ALHDCLETIDETLDELH A  DL EYP+KKSLTQ ADDLKTL+S+A+TNQETCL
Sbjct: 160 TKREKAALHDCLETIDETLDELHVAMDDLDEYPDKKSLTQHADDLKTLMSAAMTNQETCL 219

Query: 188 DGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEE 247
           DGFSHD+ADK++R+ L +GQ  VE MCSNALAMIKNMTDTDIA     M    NRKL EE
Sbjct: 220 DGFSHDDADKHVREVLLKGQRHVEHMCSNALAMIKNMTDTDIAREREAM----NRKLMEE 279

Query: 248 NNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIR 307
            ++    WP+WLSAGDRRLLQSSSV PDVVVAADGSG++KTV+AAVAAAP KSSKRY+I 
Sbjct: 280 RDESG--WPKWLSAGDRRLLQSSSVTPDVVVAADGSGDYKTVSAAVAAAPEKSSKRYIIG 339

Query: 308 IKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARD 367
           IKAGVY+ENV+V KKKTNIMF+GDGRSNTIITGS+NVVDGSTTFNSATVA VGE F+ARD
Sbjct: 340 IKAGVYKENVEVGKKKTNIMFLGDGRSNTIITGSKNVVDGSTTFNSATVAVVGEKFIARD 399

Query: 368 ITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIF 427
           ITFQNTAGPSKHQAVALRVG+DLSAFY+CDMLAYQDTLYVHSNRQFYINCL++GTVDFIF
Sbjct: 400 ITFQNTAGPSKHQAVALRVGSDLSAFYKCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIF 459

Query: 428 GNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFP 487
           GNAAAVFQDCDIHARRPNSGQKNM+TAQGR+DPNQNTGIVIQK RIGATSDL++V  SF 
Sbjct: 460 GNAAAVFQDCDIHARRPNSGQKNMLTAQGRTDPNQNTGIVIQKCRIGATSDLQAVISSFK 519

Query: 488 TFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVT 547
           T+LGRPWKEYSRTV+MQ+SI++VID  GWHEWSG+FAL+TL+YGEYQNTG GA T  RVT
Sbjct: 520 TYLGRPWKEYSRTVVMQTSITNVIDPAGWHEWSGSFALSTLYYGEYQNTGAGAGTSKRVT 579

Query: 548 WKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           WKGF+VI SA+EA+GFTPG FI G SWL +TGFP+SLGL
Sbjct: 580 WKGFKVITSASEAQGFTPGTFIAGSSWLGSTGFPYSLGL 611

BLAST of CmaCh17G006480 vs. TrEMBL
Match: H6WYP0_GOSHI (Pectinesterase OS=Gossypium hirsutum GN=PME2 PE=2 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 6.4e-237
Identity = 422/578 (73.01%), Postives = 489/578 (84.60%), Query Frame = 1

Query: 7   AGISSSGNHLPKPKYNRKKLIILAVSSTLL--LVGIVAGVVSSRNAGGEESKSHAIVKST 66
           + IS+S  H+   K ++K  + L  S  ++  ++GIVAGV S  N+   ++  HA+VKS 
Sbjct: 9   SNISNSAKHISFTKKHKKIFLALFASLVIVAAIIGIVAGVSSRNNSDESDTSHHAVVKSA 68

Query: 67  CSSTLYPDLCFSSILKNDEAM-KKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNIS 126
           CS T YPDLCFS++        KKV ++KDVIE+S+NIT  AVE NYFK+ KL   K+++
Sbjct: 69  CSGTFYPDLCFSAVTTVPAGTAKKVRSQKDVIELSLNITTTAVEHNYFKIKKLLARKDLT 128

Query: 127 HREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLD 186
            REK ALHDCLETIDETLDELH+A  DLHEYPNKKSLTQ ADDLKTL+S+A+TNQETCLD
Sbjct: 129 TREKTALHDCLETIDETLDELHEAVEDLHEYPNKKSLTQHADDLKTLMSAAMTNQETCLD 188

Query: 187 GFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEEN 246
           GFSH+ ADK +R+ L +G+  VE+MCSNALAMIKNMTDTDIAN    M  ++NRKLKE+ 
Sbjct: 189 GFSHEGADKKIREVLIDGEKYVEKMCSNALAMIKNMTDTDIAN--EMMLKSSNRKLKEDE 248

Query: 247 NDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRI 306
           +   I WPEWLSAGDRRLLQSSSV P+VVVAADGSGNFKTV+ AVA AP KSSKRY+IRI
Sbjct: 249 SG--IAWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFKTVSEAVAKAPEKSSKRYIIRI 308

Query: 307 KAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDI 366
           KAGVYRENV+V KKK+NIMF+GDGR+ TIITGSRNVVDGSTTF+SATVAAVGE FLARDI
Sbjct: 309 KAGVYRENVEVPKKKSNIMFIGDGRTKTIITGSRNVVDGSTTFHSATVAAVGEKFLARDI 368

Query: 367 TFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFG 426
           TFQNTAGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHSNRQFY+NCL++GTVDFIFG
Sbjct: 369 TFQNTAGPSKHQAVALRVGSDLSAFYNCDMLAYQDTLYVHSNRQFYVNCLVAGTVDFIFG 428

Query: 427 NAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPT 486
           NAAAVFQ+CDIHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+ V+K+FPT
Sbjct: 429 NAAAVFQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQPVRKNFPT 488

Query: 487 FLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTW 546
           +LGRPWKEYSRTV+MQS+ISDVI   GWHEWSG+FAL TLFY EYQNTG GAST  RV W
Sbjct: 489 YLGRPWKEYSRTVVMQSTISDVIQPAGWHEWSGSFALKTLFYAEYQNTGAGASTSARVKW 548

Query: 547 KGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
            G++VI SA+EA+ FTPG+FI GGSWL +TGFPF+LGL
Sbjct: 549 GGYKVITSASEAQAFTPGRFIAGGSWLSSTGFPFALGL 582

BLAST of CmaCh17G006480 vs. TAIR10
Match: AT3G14310.1 (AT3G14310.1 pectin methylesterase 3)

HSP 1 Score: 776.9 bits (2005), Expect = 8.5e-225
Identity = 403/578 (69.72%), Postives = 464/578 (80.28%), Query Frame = 1

Query: 23  RKKLIILAVSSTLLLVGIVAGVVSSRNAGGEE----SKSHAIVKSTCSSTLYPDLCFSSI 82
            KKL++L+ +  LL V  VAG+ +  +   E+      SHA+++S+CSST YP+LC S++
Sbjct: 17  NKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAV 76

Query: 83  LKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLY-ELKNISHREKIALHDCLETI 142
           +       +++++KDVIE S+N+T+ AVE NYF V KL  + K ++ REK ALHDCLETI
Sbjct: 77  VTAGGV--ELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 136

Query: 143 DETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGFSHDEADKNLRDK 202
           DETLDELH+   DLH YP KK+L + A DLKTL+SSAITNQETCLDGFSHD+ADK +R  
Sbjct: 137 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 196

Query: 203 LKEGQIEVERMCSNALAMIKNMTDTDIANYETKMG-TAANRKLKEENNDEQIQ------- 262
           L +GQI VE MCSNALAMIKNMTDTDIAN+E K   T+ NRKLKEEN +  +        
Sbjct: 197 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAG 256

Query: 263 ------WPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRI 322
                 WP WLSAGDRRLLQ S VK D  VAADGSG FKTVAAAVAAAP  S+KRYVI I
Sbjct: 257 ELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVIHI 316

Query: 323 KAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDI 382
           KAGVYRENV+V KKK NIMFMGDGR+ TIITGSRNVVDGSTTF+SATVAAVGE FLARDI
Sbjct: 317 KAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDI 376

Query: 383 TFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFG 442
           TFQNTAGPSKHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF++ CLI+GTVDFIFG
Sbjct: 377 TFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFG 436

Query: 443 NAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPT 502
           NAA V QDCDIHARRPNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+SV+ SFPT
Sbjct: 437 NAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPT 496

Query: 503 FLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTW 562
           +LGRPWKEYS+TVIMQS+ISDVI  +GW EW+G FALNTL Y EY NTG GA T  RV W
Sbjct: 497 YLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKW 556

Query: 563 KGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           +GF+VI +A EA+ +T G+FIGGG WL +TGFPFSLGL
Sbjct: 557 RGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CmaCh17G006480 vs. TAIR10
Match: AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)

HSP 1 Score: 756.1 bits (1951), Expect = 1.6e-218
Identity = 393/574 (68.47%), Postives = 460/574 (80.14%), Query Frame = 1

Query: 22  NRKKLIILAVSSTLLLVGIVAGVVSSRNAGGEESK-------SHAIVKSTCSSTLYPDLC 81
           N KKLI+ + +  LLL+  + G+ ++     +  K       SHAI+KS CSSTLYP+LC
Sbjct: 16  NNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELC 75

Query: 82  FSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLY-ELKNISHREKIALHDC 141
           FS++       K+++++K+VIE S+N+T KAV+ NYF V KL  + K ++ RE  ALHDC
Sbjct: 76  FSAVAAT--GGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 135

Query: 142 LETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGFSHDEADKN 201
           LETIDETLDELH A  DLH+YP +KSL + ADDLKTL+SSAITNQ TCLDGFS+D+AD+ 
Sbjct: 136 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 195

Query: 202 LRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAA-----NRKLKEENND-EQ 261
           +R  L +GQ+ VE MCSNALAMIKNMT+TDIAN+E +  ++      NRKLKE   D + 
Sbjct: 196 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDS 255

Query: 262 IQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRIKAGV 321
             WP+WLS GDRRLLQ S++K D  VA DGSG+F TVAAAVAAAP KS+KR+VI IKAGV
Sbjct: 256 DGWPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKAGV 315

Query: 322 YRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQN 381
           YRENV+V KKKTNIMF+GDGR  TIITGSRNVVDGSTTF+SATVAAVGE FLARDITFQN
Sbjct: 316 YRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQN 375

Query: 382 TAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAA 441
           TAGPSKHQAVALRVG+D SAFYQCDM AYQDTLYVHSNRQF++ C I+GTVDFIFGNAAA
Sbjct: 376 TAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAAA 435

Query: 442 VFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPTFLGR 501
           V QDCDI+ARRPNSGQKNMVTAQGRSDPNQNTGIVIQ  RIG TSDL +V+ +FPT+LGR
Sbjct: 436 VLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGR 495

Query: 502 PWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGFR 561
           PWKEYSRTVIMQS ISDVI  +GWHEWSG+FAL+TL Y EY N G GA T  RV WKG++
Sbjct: 496 PWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYK 555

Query: 562 VIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           VI S TEA+ FT G+FIGGG WL +TGFPFSL L
Sbjct: 556 VITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CmaCh17G006480 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 431.8 bits (1109), Expect = 6.7e-121
Identity = 262/547 (47.90%), Postives = 343/547 (62.71%), Query Frame = 1

Query: 35  LLLVGIVAGVVSSRNAGGEESKSHAIVKSTCSSTLYPDLCFSSILKNDEAMKKVSNKKDV 94
           ++L  +VA  VS  N           VK+ CS T  P  C    L ++   + + ++ + 
Sbjct: 12  VILCILVASTVSGYNQKD--------VKAWCSQTPNPKPC-EYFLTHNSNNEPIKSESEF 71

Query: 95  IEVSINITVKAVEENYFKVLKLYELKNISHREKIALHDCLETIDETLDELHKATVDLHEY 154
           +++S+ + +            L   K    REK A  DC++  D T+ ++++ T+D    
Sbjct: 72  LKISMKLVLDRAILAKTHAFTLGP-KCRDTREKAAWEDCIKLYDLTVSKINE-TMD---- 131

Query: 155 PNKKSLTQQADDLKTLLSSAITNQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALA 214
           PN K       D +T LS+A+TN +TC  GF            L+ G  ++       L 
Sbjct: 132 PNVKCSKL---DAQTWLSTALTNLDTCRAGF------------LELGVTDI------VLP 191

Query: 215 MIKNMTDTDIANYETKMGTAANRKLKEENNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVA 274
           ++ N     + N       A N+        E+  +P W+  GDR+LLQSS+ K + VVA
Sbjct: 192 LMSNNVSNLLCNT-----LAINKVPFNYTPPEKDGFPSWVKPGDRKLLQSSTPKDNAVVA 251

Query: 275 ADGSGNFKTVAAAVAAAPVKSSKRYVIRIKAGVYRENVDVQKKKTNIMFMGDGRSNTIIT 334
            DGSGNFKT+  A+ AA    S R+VI +K GVY EN++++KK  N+M  GDG   TIIT
Sbjct: 252 KDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEIRKK--NVMLRGDGIGKTIIT 311

Query: 335 GSRNVVDGSTTFNSATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDML 394
           GS++V  G+TTFNSATVAAVG+GF+AR ITF+NTAG S  QAVALR G+DLS FYQC   
Sbjct: 312 GSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFE 371

Query: 395 AYQDTLYVHSNRQFYINCLISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSD 454
           AYQDTLYVHSNRQFY +C + GTVDFIFGNAAAV Q+C+I ARRP S + N +TAQGRSD
Sbjct: 372 AYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPRS-KTNTITAQGRSD 431

Query: 455 PNQNTGIVIQKSRIGATSDLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEW 514
           PNQNTGI+I  SR+ A SDLR V  S  T+LGRPW++YSRTV M++S+  +ID +GW EW
Sbjct: 432 PNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEW 491

Query: 515 SGNFALNTLFYGEYQNTGPGASTGGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATG 574
            GNFAL TLFY E+QNTGPGAST GRVTW GFRV+ SA+EA  FT G F+ GGSW+  + 
Sbjct: 492 DGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWI-PSS 511

Query: 575 FPFSLGL 582
            PF+ GL
Sbjct: 552 VPFTSGL 511

BLAST of CmaCh17G006480 vs. TAIR10
Match: AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)

HSP 1 Score: 431.4 bits (1108), Expect = 8.8e-121
Identity = 250/581 (43.03%), Postives = 355/581 (61.10%), Query Frame = 1

Query: 20  KYNRKKLIILAVSSTLLLV---GIVAGVVSSRNAGGEES---KSHAIVKSTCSSTLYPDL 79
           K  + KLI+  ++  ++ V   GI AG+ +  +   E     K    +  TCS +LYP+L
Sbjct: 28  KKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNL 87

Query: 80  CFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNISH-----REKI 139
           C  ++L  D      +++ ++I +S N T++   +       LY    I++     R + 
Sbjct: 88  CIDTLL--DFPGSLTADENELIHISFNATLQKFSK------ALYTSSTITYTQMPPRVRS 147

Query: 140 ALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGFSHD 199
           A   CLE +D+++D L +A   +      +S +    D+ T LSSA+TN +TC DGF   
Sbjct: 148 AYDSCLELLDDSVDALTRALSSVVVVSGDESHS----DVMTWLSSAMTNHDTCTDGFDEI 207

Query: 200 EADKN-LRDKLKEGQIEVERMCSNALAM----IKNMTDTDIANYETKMGTAANRKLKEEN 259
           E     ++D++     ++  M SN LA+    +K+++   + N         NRKL    
Sbjct: 208 EGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN---------NRKLLGTE 267

Query: 260 NDEQIQWPEWLSAGDRRLL--QSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVI 319
             E++  P WL   DR LL   +S+++ D+ V+ DGSG FKT+A A+  AP  SS+R+VI
Sbjct: 268 ETEEL--PNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVI 327

Query: 320 RIKAGVYRE-NVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLA 379
            +KAG Y E N+ V +KKTN+MF+GDG+  T+ITG +++ D  TTF++AT AA G GF+ 
Sbjct: 328 YVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIV 387

Query: 380 RDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDF 439
           RD+TF+N AGP+KHQAVALRVG D +  Y+C+++ YQD LYVHSNRQF+  C I GTVDF
Sbjct: 388 RDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDF 447

Query: 440 IFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKS 499
           IFGNAA + Q C+I+AR+P + QK  +TAQ R DPNQNTGI I   ++ AT DL + + S
Sbjct: 448 IFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGS 507

Query: 500 FPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGR 559
           +PT+LGRPWK YSR V M S + D ID +GW EW+G FAL++L+YGEY N G G+  G R
Sbjct: 508 YPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQR 567

Query: 560 VTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           V W G+ VI S  EA  FT  +FI G SWL +TG  F  GL
Sbjct: 568 VKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of CmaCh17G006480 vs. TAIR10
Match: AT1G11580.1 (AT1G11580.1 methylesterase PCR A)

HSP 1 Score: 427.9 bits (1099), Expect = 9.7e-120
Identity = 263/576 (45.66%), Postives = 342/576 (59.38%), Query Frame = 1

Query: 16  LPKPKYNRKKLIILAVSSTLLLVGIV---AGVVSSRNAGGEESKSHAIVKSTCSSTLYPD 75
           L KPK  + K + L +S   +L  +    A ++S      ++S         C      D
Sbjct: 9   LSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSL--LTTSQICHGAHDQD 68

Query: 76  LCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNISHREKIALHD 135
            C + + +         N+ D++ V +  +V  +E     V +   +++   R+K    D
Sbjct: 69  SCQALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEA-RIRSNGVRDKAGFAD 128

Query: 136 CLETID-------ETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGF 195
           C E +D        +++EL     +L  Y N          + T LSS +TN  TCL+  
Sbjct: 129 CEEMMDVSKDRMMSSMEELRGGNYNLESYSN----------VHTWLSSVLTNYMTCLESI 188

Query: 196 SHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEENND 255
           S  +   N +  +K    ++E + S A   +        A  + KM   +NR        
Sbjct: 189 S--DVSVNSKQIVKP---QLEDLVSRARVALAIFVSVLPARDDLKM-IISNR-------- 248

Query: 256 EQIQWPEWLSAGDRRLLQSS----SVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVI 315
               +P WL+A DR+LL+SS     V  +VVVA DG+G FKTV  AVAAAP  S+ RYVI
Sbjct: 249 ----FPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVI 308

Query: 316 RIKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLAR 375
            +K GVY+E +D+ KKK N+M +GDG+  TIITGS NV+DGSTTF SATVAA G+GF+A+
Sbjct: 309 YVKKGVYKETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQ 368

Query: 376 DITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFI 435
           DI FQNTAGP+KHQAVALRV AD +   +C + AYQDTLY H+ RQFY +  I+GTVDFI
Sbjct: 369 DIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFI 428

Query: 436 FGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSF 495
           FGN+A VFQ+CDI AR P +GQKNM+TAQGR D NQNT I IQK +I A+SDL  V+ S 
Sbjct: 429 FGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSV 488

Query: 496 PTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRV 555
            TFLGRPWK YSRTVIMQS I + ID  GW  W G FAL+TL+YGEY NTGPGA T  RV
Sbjct: 489 KTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRV 548

Query: 556 TWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPF 578
            WKGF+VI+ + EAE FT  K I GG WL+ TG  F
Sbjct: 549 NWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTF 553

BLAST of CmaCh17G006480 vs. NCBI nr
Match: gi|449447960|ref|XP_004141734.1| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 967.6 bits (2500), Expect = 9.6e-279
Identity = 495/589 (84.04%), Postives = 537/589 (91.17%), Query Frame = 1

Query: 1   MEQPIAAG--ISSSGNHLPKPKYNRKKLIILAVSSTLLLVGIVAGVVS--SRNAGGEESK 60
           MEQPIAAG  +S+S +     K ++KKL+I+ + STLL+VG V G+V+  ++++     +
Sbjct: 7   MEQPIAAGAGVSNSSDLQKTQKSSKKKLLIVTLCSTLLIVGAVIGIVAGTTKSSSKYSGE 66

Query: 61  SHAIVKSTCSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKL 120
           SHAIVKSTCSSTLYPD+C+S+I +++ AM KV N+KDVIE+SINITVKAVEENYF+V KL
Sbjct: 67  SHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKL 126

Query: 121 YELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAIT 180
             LKNISHRE+IALHDCLETIDETLDELHKA VDL+EYPNKKSL Q ADDLKTLLSSAIT
Sbjct: 127 CGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAIT 186

Query: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAAN 240
           NQETCLDGFSHD+ADKNLRDKLKEGQ+EVE MCSNALAMIKNMTDTDIANYE KMG   N
Sbjct: 187 NQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKN 246

Query: 241 RKLKEE----NNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAP 300
           RKL  E    NND+ IQWPEWLSAGDRRLLQSSSV P+VVVAADGSGNF+TVAAAVAAAP
Sbjct: 247 RKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAP 306

Query: 301 VKSSKRYVIRIKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVA 360
           V+SSKRYVIRIKAGVYRENV+V KKKTNIMF+GDGR NTIITGSRNVVDGSTTFNSAT+A
Sbjct: 307 VRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMA 366

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 367 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 426

Query: 421 LISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           L+SGTVDFIFGNAAA+FQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 427 LVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 486

Query: 481 DLRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTG 540
           DLR VQKSFPTFLGRPWKEYSRTVIMQ +ISDVID KGWHEWSG+FALNTLFYGEYQNTG
Sbjct: 487 DLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTG 546

Query: 541 PGASTGGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
            GASTGGRVTWKGFRVIRSATEAE FT GKFIGGGSWL +TGFPFSLGL
Sbjct: 547 AGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595

BLAST of CmaCh17G006480 vs. NCBI nr
Match: gi|659124992|ref|XP_008462453.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 961.4 bits (2484), Expect = 6.9e-277
Identity = 491/563 (87.21%), Postives = 521/563 (92.54%), Query Frame = 1

Query: 24  KKLIILAVSSTLLLVGIVAGVVSSRNAGGEES-KSHAIVKSTCSSTLYPDLCFSSILKND 83
           KKL+IL +SSTLL+VG V G+V+      ++S KSHAIVKSTCSSTLYPD+C+S+I K++
Sbjct: 36  KKLLILTLSSTLLIVGAVIGIVAGTTKSSKDSGKSHAIVKSTCSSTLYPDMCYSAISKSE 95

Query: 84  EAMKKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNISHREKIALHDCLETIDETLD 143
            AM KV ++KDVIEVSINITVKAVEENYF+V KL  LKNISHREKIALHDCLETIDETLD
Sbjct: 96  GAMLKVKSQKDVIEVSINITVKAVEENYFRVKKLCGLKNISHREKIALHDCLETIDETLD 155

Query: 144 ELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLDGFSHDEADKNLRDKLKEGQ 203
           ELHKA VDL+EYPNKKSL Q ADDLKTLLSSAITNQETCLDGFSHD ADK LRDKLK GQ
Sbjct: 156 ELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITNQETCLDGFSHDNADKTLRDKLKAGQ 215

Query: 204 IEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKL----KEENNDEQIQWPEWLSAGD 263
           +EVE MCSNALAMIKNMTDTDIANYE KMG   NRKL    ++ NN+++IQWPEWLSAGD
Sbjct: 216 MEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNRKLMAEEEDNNNNDEIQWPEWLSAGD 275

Query: 264 RRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRIKAGVYRENVDVQKKK 323
           RRLLQSSSV P+VVVAADGSGNF+TVAAAVAAAP KSSKRYVIRIKAGVYRENV+V KKK
Sbjct: 276 RRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPEKSSKRYVIRIKAGVYRENVEVPKKK 335

Query: 324 TNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNTAGPSKHQAVA 383
           TNIMFMGDGR NTIITGSRNVVDGSTTF SATVAAVGEGFLARDITFQNTAGPSKHQAVA
Sbjct: 336 TNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVAAVGEGFLARDITFQNTAGPSKHQAVA 395

Query: 384 LRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAAVFQDCDIHARR 443
           LRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL+SGTVDFIFGNAAA+FQDCDIHAR+
Sbjct: 396 LRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVSGTVDFIFGNAAAIFQDCDIHARK 455

Query: 444 PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPTFLGRPWKEYSRTVIM 503
           PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLR VQKSFPTFLGRPWKEYSRTVIM
Sbjct: 456 PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKSFPTFLGRPWKEYSRTVIM 515

Query: 504 QSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGFRVIRSATEAEGF 563
           Q SISDVID KGWHEWSG+FALNTLFYGEYQNTGPGASTGGRVTWKGFRVIRSATEAEGF
Sbjct: 516 QCSISDVIDPKGWHEWSGSFALNTLFYGEYQNTGPGASTGGRVTWKGFRVIRSATEAEGF 575

Query: 564 TPGKFIGGGSWLRATGFPFSLGL 582
           T GKFIGGGSWLR+TGFPFSLGL
Sbjct: 576 TAGKFIGGGSWLRSTGFPFSLGL 598

BLAST of CmaCh17G006480 vs. NCBI nr
Match: gi|590621367|ref|XP_007024770.1| (Pectin methylesterase 3 isoform 1 [Theobroma cacao])

HSP 1 Score: 839.0 bits (2166), Expect = 5.2e-240
Identity = 433/578 (74.91%), Postives = 489/578 (84.60%), Query Frame = 1

Query: 9   ISSSGNHLPKPKYNRKKLIILAVSSTLLLV----GIVAGVVSSRNAGGEESKSHAIVKST 68
           IS SG H+   K ++K  +ILAV  +L++V    GIVAGV S  N+   ++  HAIVKS 
Sbjct: 11  ISDSGKHISSTKKHKK--VILAVFGSLVIVAAIIGIVAGVNSRNNSDETDTSHHAIVKSA 70

Query: 69  CSSTLYPDLCFSSILKNDEAM-KKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNIS 128
           CS T YPDLCFS +     A  KKV +KKDVIE+S+NIT  AVE NYFK+ KL   K ++
Sbjct: 71  CSITRYPDLCFSEVAAAPAATSKKVKSKKDVIELSLNITTTAVEHNYFKIKKLLAKKGLT 130

Query: 129 HREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLD 188
            REK ALHDCLETIDETLDELH+A  DLHEYPNKKSLTQ ADDLKTL+S+A+TNQETCLD
Sbjct: 131 EREKTALHDCLETIDETLDELHEAVEDLHEYPNKKSLTQHADDLKTLMSAAMTNQETCLD 190

Query: 189 GFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEEN 248
           GFSHD ADK +R  L +G+  VERMCSNALAMIKNMTDTDIAN   K  T++NRKLKE+ 
Sbjct: 191 GFSHDAADKKIRKVLIDGEKYVERMCSNALAMIKNMTDTDIANEMLK--TSSNRKLKEQE 250

Query: 249 NDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRI 308
           N   I WPEWLSAGDRRLLQSSSV PDVVVAADGSGN+KTV+ AVA AP +SSKRYVI+I
Sbjct: 251 NG--IAWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNYKTVSEAVAKAPQRSSKRYVIKI 310

Query: 309 KAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDI 368
           KAGVYRENV+V KKKTNIMF+GDGR+ TIITGSRNVVDGSTTF+SATVA VGE FLARDI
Sbjct: 311 KAGVYRENVEVPKKKTNIMFLGDGRTETIITGSRNVVDGSTTFHSATVAVVGERFLARDI 370

Query: 369 TFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFG 428
           TFQNTAGPSKHQAVALRVGADLSAFY+CDMLAYQDTLY HSNRQFY+NC+I+GTVDFIFG
Sbjct: 371 TFQNTAGPSKHQAVALRVGADLSAFYECDMLAYQDTLYAHSNRQFYVNCIIAGTVDFIFG 430

Query: 429 NAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPT 488
           NAAAVFQ+CDIHARRPNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+SV+ +FPT
Sbjct: 431 NAAAVFQNCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVRSNFPT 490

Query: 489 FLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTW 548
           +LGRPWKEYSRTV+MQS ISDVI   GWHEWS +FAL TLFYGEYQNTG GA T GRV W
Sbjct: 491 YLGRPWKEYSRTVVMQSVISDVIHPAGWHEWSESFALRTLFYGEYQNTGAGAGTSGRVKW 550

Query: 549 KGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
           +G++VI SA+EA+ ++PG+FI GGSWL +TGFPFSLGL
Sbjct: 551 EGYKVITSASEAQAYSPGRFIAGGSWLSSTGFPFSLGL 582

BLAST of CmaCh17G006480 vs. NCBI nr
Match: gi|401834530|gb|AFQ23194.1| (pectin methylesterase [Theobroma cacao])

HSP 1 Score: 834.3 bits (2154), Expect = 1.3e-238
Identity = 431/577 (74.70%), Postives = 486/577 (84.23%), Query Frame = 1

Query: 9   ISSSGNHLPKPKYNRKKLIILAVSSTLLLV----GIVAGVVSSRNAGGEESKSHAIVKST 68
           IS SG H+   K ++K  +ILAV  +L++V    GIVAGV S  N+   ++  HAIVKS 
Sbjct: 11  ISDSGKHISSTKKHKK--VILAVFGSLVIVAAIIGIVAGVNSRNNSDETDTSHHAIVKSA 70

Query: 69  CSSTLYPDLCFSSILKNDEAM-KKVSNKKDVIEVSINITVKAVEENYFKVLKLYELKNIS 128
           CS T YPDLCFS +     A  KKV +KKDVIE+S+NIT  AVE NYFK+ KL   K ++
Sbjct: 71  CSITRYPDLCFSEVAAAPAATSKKVKSKKDVIELSLNITTTAVEHNYFKIKKLLAKKGLT 130

Query: 129 HREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAITNQETCLD 188
            REK ALHDCLETIDETLDELH+A  DLHEYPNKKSLTQ ADDLKTL+S+A+TNQETCLD
Sbjct: 131 EREKTALHDCLETIDETLDELHEAVEDLHEYPNKKSLTQHADDLKTLMSAAMTNQETCLD 190

Query: 189 GFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAANRKLKEEN 248
           GFSHD ADK +R  L +G+  VERMCSNALAMIKNMTDTDIAN   K  T++NRKLKE+ 
Sbjct: 191 GFSHDAADKKIRKVLIDGEKYVERMCSNALAMIKNMTDTDIANEMLK--TSSNRKLKEQE 250

Query: 249 NDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSSKRYVIRI 308
           N   I WPEWLSAGDRRLLQSSSV PDVVVAADGSGN+KTV+ AVA AP +SSKRYVI+I
Sbjct: 251 NG--IAWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNYKTVSEAVAKAPQRSSKRYVIKI 310

Query: 309 KAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDI 368
           KAGVYRENV+V KKKTNIMF+GDGR+ TIITGSRNVVDGSTTF+SATVA VGE FLAR I
Sbjct: 311 KAGVYRENVEVPKKKTNIMFLGDGRTETIITGSRNVVDGSTTFHSATVAVVGERFLARSI 370

Query: 369 TFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFG 428
           TFQNTAGPSKHQAVALRVGADLSAFY+CDMLAYQDTLY HSNRQFY+NC+I+GTVDFIFG
Sbjct: 371 TFQNTAGPSKHQAVALRVGADLSAFYECDMLAYQDTLYAHSNRQFYVNCIIAGTVDFIFG 430

Query: 429 NAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRSVQKSFPT 488
           NAAAVFQ+CDIHARRPNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+SV  +FPT
Sbjct: 431 NAAAVFQNCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVTSNFPT 490

Query: 489 FLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTW 548
           +LGRPWKEYSRTV+MQS ISDVI   GWHEWS +FAL TLFYGEYQNTG GA T GRV W
Sbjct: 491 YLGRPWKEYSRTVVMQSVISDVIHPAGWHEWSESFALRTLFYGEYQNTGAGAGTSGRVKW 550

Query: 549 KGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLG 581
           +G++VI SA+EA+ ++PG+FI GGSWL +TGFPFSLG
Sbjct: 551 EGYKVITSASEAQAYSPGRFIAGGSWLSSTGFPFSLG 581

BLAST of CmaCh17G006480 vs. NCBI nr
Match: gi|698531130|ref|XP_009762395.1| (PREDICTED: pectinesterase/pectinesterase inhibitor U1 [Nicotiana sylvestris])

HSP 1 Score: 831.6 bits (2147), Expect = 8.2e-238
Identity = 431/586 (73.55%), Postives = 491/586 (83.79%), Query Frame = 1

Query: 7   AGISSSGNHLPKPKYNRKKLIILAVSSTLLL---VGIVAGVVSSRNAGGEE-------SK 66
           AG+  SG ++   K  +KKL +  V+S LL+   +G+V GV    N   +        S 
Sbjct: 9   AGMLDSGKNVNFSK-GKKKLFLTVVASVLLVAAVIGVVVGVKFRSNNSDDHADIQAITSA 68

Query: 67  SHAIVKSTCSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKL 126
           +HAIVKS C +TL+P+LC+S+I    +  KKV+++KDVIE+S+NIT +AV+ N+FKV KL
Sbjct: 69  AHAIVKSACENTLHPELCYSTIASVSDFSKKVTSQKDVIELSLNITCRAVQHNFFKVEKL 128

Query: 127 YEL-KNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAI 186
            +  K +  REK+ALHDCLETIDETLDELH A  DL  YPNKKSL   ADDLKTL+SSAI
Sbjct: 129 IKTRKGLKPREKVALHDCLETIDETLDELHTAIKDLELYPNKKSLKAHADDLKTLISSAI 188

Query: 187 TNQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAA 246
           TNQETCLDGFSHD+ADK +R  L +GQ  VE+MCSNALAMI NMTDTDIAN +   GT  
Sbjct: 189 TNQETCLDGFSHDDADKKVRKALLKGQKHVEKMCSNALAMICNMTDTDIANEQKLKGTTT 248

Query: 247 NRKLKEENNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKS 306
           NRKL+E+N+    +WPEWLSAGDRRLLQSS+V+PDVVVAADGSGNFKTV+ AVA AP KS
Sbjct: 249 NRKLREDNS----EWPEWLSAGDRRLLQSSTVRPDVVVAADGSGNFKTVSEAVAKAPEKS 308

Query: 307 SKRYVIRIKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVG 366
           SKRYVIRIKAGVYRENVDV KKKTNIMFMGDGRSNTIITGSRNV DGSTTF+SATVAAVG
Sbjct: 309 SKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVKDGSTTFHSATVAAVG 368

Query: 367 EGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLIS 426
           E FLARDITFQNTAG +KHQAVALRVG+DLSAFY+CD+LAYQD+LYVHSNRQ+++ CLI+
Sbjct: 369 EKFLARDITFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQYFVQCLIA 428

Query: 427 GTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLR 486
           GTVDFIFGNAAAV QDCDIHARRP SGQKNMVTAQGRSDPNQNTGIVIQK RIGATSDLR
Sbjct: 429 GTVDFIFGNAAAVLQDCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLR 488

Query: 487 SVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGA 546
            VQKSFPT+LGRPWKEYSRTVIMQSSI+DVI+S GWHEW+GNFALNTLFYGEYQNTG GA
Sbjct: 489 PVQKSFPTYLGRPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGEYQNTGAGA 548

Query: 547 STGGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 582
            T GRV WKGF+VI SATEA+ +TPG+FI GGSWL +TGFPFSLGL
Sbjct: 549 GTSGRVKWKGFKVITSATEAQAYTPGRFIAGGSWLSSTGFPFSLGL 589

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME3_CITSI2.8e-23874.69Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME1_CITSI1.1e-23774.35Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
PMEU1_SOLLC9.1e-22972.68Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2... [more]
PME3_ARATH1.5e-22369.72Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... [more]
PME2_ARATH2.8e-21768.47Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KAM6_CUCSA6.7e-27984.04Pectinesterase OS=Cucumis sativus GN=Csa_7G447990 PE=4 SV=1[more]
A0A061GE06_THECC3.6e-24074.91Pectinesterase OS=Theobroma cacao GN=TCM_029251 PE=4 SV=1[more]
J7HYD0_THECC8.9e-23974.70Pectinesterase OS=Theobroma cacao GN=PME PE=2 SV=1[more]
F6HXK8_VITVI2.9e-23774.27Pectinesterase OS=Vitis vinifera GN=VIT_09s0002g00320 PE=4 SV=1[more]
H6WYP0_GOSHI6.4e-23773.01Pectinesterase OS=Gossypium hirsutum GN=PME2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.18.5e-22569.72 pectin methylesterase 3[more]
AT1G53830.11.6e-21868.47 pectin methylesterase 2[more]
AT2G45220.16.7e-12147.90 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G53370.18.8e-12143.03 pectin methylesterase PCR fragment F[more]
AT1G11580.19.7e-12045.66 methylesterase PCR A[more]
Match NameE-valueIdentityDescription
gi|449447960|ref|XP_004141734.1|9.6e-27984.04PREDICTED: pectinesterase-like [Cucumis sativus][more]
gi|659124992|ref|XP_008462453.1|6.9e-27787.21PREDICTED: pectinesterase-like [Cucumis melo][more]
gi|590621367|ref|XP_007024770.1|5.2e-24074.91Pectin methylesterase 3 isoform 1 [Theobroma cacao][more]
gi|401834530|gb|AFQ23194.1|1.3e-23874.70pectin methylesterase [Theobroma cacao][more]
gi|698531130|ref|XP_009762395.1|8.2e-23873.55PREDICTED: pectinesterase/pectinesterase inhibitor U1 [Nicotiana sylvestris][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR006501Pectinesterase_inhib_dom
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
IPR018040Pectinesterase_Tyr_AS
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G006480.1CmaCh17G006480.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 270..566
score: 1.8E
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 58..220
score: 3.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 60..215
score: 3.9
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 56..215
score: 1.3
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 32..219
score: 2.1
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 60..220
score: 1.83
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 266..580
score: 7.85E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 266..579
score: 3.2E
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 295..314
scor
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 24..581
score:
NoneNo IPR availablePANTHERPTHR31707:SF55PECTINESTERASE 2-RELATEDcoord: 24..581
score:

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh17G006480Cucsa.149200Cucumber (Gy14) v1cgycmaB0411
CmaCh17G006480Cucsa.359780Cucumber (Gy14) v1cgycmaB0983
CmaCh17G006480Cla012555Watermelon (97103) v1cmawmB342
CmaCh17G006480Cla015505Watermelon (97103) v1cmawmB330
CmaCh17G006480Csa3G646640Cucumber (Chinese Long) v2cmacuB362
CmaCh17G006480Csa7G447990Cucumber (Chinese Long) v2cmacuB383
CmaCh17G006480MELO3C024306Melon (DHL92) v3.5.1cmameB319
CmaCh17G006480MELO3C022704Melon (DHL92) v3.5.1cmameB334
CmaCh17G006480ClCG09G001400Watermelon (Charleston Gray)cmawcgB308
CmaCh17G006480ClCG07G014280Watermelon (Charleston Gray)cmawcgB330
CmaCh17G006480CSPI03G28630Wild cucumber (PI 183967)cmacpiB366
CmaCh17G006480CSPI07G22040Wild cucumber (PI 183967)cmacpiB387
CmaCh17G006480CmoCh14G012490Cucurbita moschata (Rifu)cmacmoB352
CmaCh17G006480CmoCh17G006310Cucurbita moschata (Rifu)cmacmoB353
CmaCh17G006480Lsi07G006410Bottle gourd (USVL1VR-Ls)cmalsiB360
CmaCh17G006480Lsi02G028550Bottle gourd (USVL1VR-Ls)cmalsiB345
CmaCh17G006480Cp4.1LG12g09640Cucurbita pepo (Zucchini)cmacpeB371
CmaCh17G006480Cp4.1LG03g14830Cucurbita pepo (Zucchini)cmacpeB405
CmaCh17G006480MELO3C024306.2Melon (DHL92) v3.6.1cmamedB372
CmaCh17G006480MELO3C022704.2Melon (DHL92) v3.6.1cmamedB388
CmaCh17G006480CsaV3_7G033930Cucumber (Chinese Long) v3cmacucB0456
CmaCh17G006480CsaV3_3G031040Cucumber (Chinese Long) v3cmacucB0430
CmaCh17G006480Cla97C07G141120Watermelon (97103) v2cmawmbB380
CmaCh17G006480Cla97C09G163490Watermelon (97103) v2cmawmbB389
CmaCh17G006480Bhi09G000208Wax gourdcmawgoB0488
CmaCh17G006480Bhi01G002208Wax gourdcmawgoB0476
CmaCh17G006480CsGy7G020340Cucumber (Gy14) v2cgybcmaB919
CmaCh17G006480CsGy3G026590Cucumber (Gy14) v2cgybcmaB348
CmaCh17G006480Carg06396Silver-seed gourdcarcmaB0156
CmaCh17G006480Carg18946Silver-seed gourdcarcmaB1063
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh17G006480CmaCh14G012210Cucurbita maxima (Rimu)cmacmaB251
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh17G006480Silver-seed gourdcarcmaB0840
CmaCh17G006480Cucurbita maxima (Rimu)cmacmaB383
CmaCh17G006480Cucurbita moschata (Rifu)cmacmoB375
CmaCh17G006480Cucurbita pepo (Zucchini)cmacpeB376