CmaCh17G003710 (gene) Cucurbita maxima (Rimu)

NameCmaCh17G003710
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionGlyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a
LocationCma_Chr17 : 2070159 .. 2073082 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAATTCAACTGTAACTCTGCCCATGCCTACTTTTTTTTTATATTTATCCTTTGTTCATGATTTTCTCGTTTTCCAATACGCAATCTTGCAAGAACTACCCCATGCCTACTTTTTTATTCAATCTCCACTCCCCAATTTGATCACACCGACTTCCACTTTCTGCTGTATTCGCTCCTGATTTCTTCGCCGGAATGGATCGATCATCGTCGGAAAAGCTCATCCTCGTCAACCGGGAACCAAACGGGATCGCATTCGTTACGATCAACCGACCGAAGTCCTTGAATTCGTTAACGAGAGCGATGATGGCAGATTTGGCTCAGGCGTTTAAGAGCTTGGACCGCGACGAGTCCGTGCGAGTTATCGTGCTCTCTGGAACAGGCCGAGCCTTCTGTTCCGGTGTAGACCTTACTGCGGCGGAGGATGTTTTCAAAGGAGATGTGAAGGATGTTGAAGCCGATCCGGTTGTGCAAATGGAACGCTGCCGGAAGCCGATAATCGGAGCGGTTGCAGGTTTCGCCATCACCGCTGGATTTGAGATCGCCTTGGCTTGCGACATACTCATAGCTGCCAAAGGAGCTAAGTTCATCGATACTCATGCTAGGTTTGTTATTTTCCGACTCAATTTTTTTGTAATCTCGACTGATTCTTGCTGCTAGGAATAGGATAAACACGGATTTAGGATCAATAAACCAAGTGATTTTCTGAAATCTCCTGCTAATTCTGTTCTATTGAAACTCCGAATTTCTGGAAAATTTCGTGATAGATTTCGGATATTTCCTTCATGGGGATTGTCTCAGAAACTCCCGAGGATCATCGGAGTTAACAAAGCTCGTGAAGTATCCTTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGAGGAAGGGTTTGGTGAATCACGTGGTCGACGGAGGTGAATTGTTGAAGAAAACTCGAGAGATTGCGGAGGCCATCTTGAAGAACAATCAGGACTTGGTGTTGAGATACAAGTCCGTCATTAACGATGGCCTGAAGCTGGATCTTGGCCATGCTCTTGCGCTGGAGAAGGTAATGTCCTCGACAAAATTCATATTCCGAATCTGTTGATCATTGAACCTTGAAAATTGTGTTCTTATTTCTGTTAAACTCGAGTTTCTCTTCGAATTTTTGTGCCAATAACTGTCTGGGGATGATTTTTTAAGTACTTTTTGGATGAACAAAGCTTGTGTAGTAGCATCCTGCAACTAGGGATGTCCAATGTTCATTTACACCATGGGGAATTGCTCCGAATGGGGTTTCCTCTTTACACAGGAAATGAGGAAGGAGGAGCAGGGAGAATTTTTGAAGAATATTCATCGTCTCCACTCTTGTTCCCGTCCTTGCCCCGAATGGGGTTTCCTCCCTACACAGGAAGTTGGGAAGGAGGAGAAAGGGGAATTTTCGAAGAATATTCGTTGTCCCTGCTCGGGGCGTTCCTCTTTACACAGGAAGTGGGGAAGGAGGAGGAAGTTGGTTTTTAGTGTTTGGTGGTGTTCGGCACAGGGGAGAACATCCAAGCCGTGGAATTCACACGAAAGGAAACGAGGAATTATCAACTAAAAAAGTGCTCTCTGAACATGAAAGTTGTTTTCCTCTTGGAAAAGTGTTCCTCGAAGATCCTTACCCTTCCACTTCCCCTTTCTTCCCATGTTTCCGCTGCTCTCGTTCTCGTCCTTGGCCTAAATAGGGCTTCCTCTTTACACAAGAAGTGGGGAAGGAGGAGGAAGTTGGTTTTTAGTGTTTGGTGGTGTTCTAGGGGAGAACATCCAAGCTGTGAAATTCACACGAGACGGAACGAGGAACGATCAACGAAAAAAGTGCTCTCTAAACGTGAAAGTTTTCCTACTGGGAAAGTCTCCCTCGACACAAAGTTCCTTACCCTTCCACCTTCACTTTCTTCCCTTGTTTCCAGTACTCTCGCTCCCGTCCTTGCCCCGTTTCTTTTATCTAAATGGAGAATAATTCTTGACGAGGAATCCTCATCCCAATACTAAACTTTGCGGGGATGGAAACGAAGCATTTCTACCTATACAACTGACTGTTTGTTTCGTTTGTTTGTTTGTTTTGTTGATGCAGGAGAGAGCTCATGCATATTACAATGGAATGACCAAAGAGCAATTCAAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAGCCTTCCAAGTTATAGTCTAATCGATGTAGCTTACTGTCTGTTTCTACACTGCAACAATAATAAGAAGAACTTGCCTCCTAAACGGTTAGTGGATTCTTCCCTTGTTTTATTCACATGTAATTTGATTTTAGTGGATTAGAGATAAAATGACTATTTTGAGAAGAATTCAGGCTGGTTTCTGAGCAATAAATGTTGGGTATTTTATGGATAATGGCTGAATATGAACTCATTGGATGGCAGTTTATGGTTGATTTGATATTACATTGTAAGGGTAGATTATAAATAACAATTATTTGTAAAGGAAGAATCCACAAGACATTCAGAACAGGATATATAAATGTTTAACATGACCTTCCCCCACCCAACTGAGTGGTGATGGTTCATGAACACAGTTAAGAGAGAGGTAAAATGTGAAGTCCTCACGTCGGTTAGAGAGAGGAACGAAAGGGTGTGGAAATCTTTTCCTAGTAGATGTGTTTTAAAACTTTGAGAACAAGCTTGAAAGGGAAAATCCAAAAAGAACAATATTTGTTAGTGGTGTGGCATGAGCTGTTAGAAATGGTGTCAGAGCAAGACATCGGGGGTGTGTCAACGAAGACGTTGGGTTCCTATGGAGATGGATTGTGAGATCCCACATCCGTTGGCCAGACACTGAGCAGTGTCAAACCAAAATACCCAACAATCGCCAGGTTGTCTCTTACCTGGCCATGGATTCAGCAGCAGTTTGAAGAGGGGAACAAAAC

mRNA sequence

TGAATTCAACTGTAACTCTGCCCATGCCTACTTTTTTTTTATATTTATCCTTTGTTCATGATTTTCTCGTTTTCCAATACGCAATCTTGCAAGAACTACCCCATGCCTACTTTTTTATTCAATCTCCACTCCCCAATTTGATCACACCGACTTCCACTTTCTGCTGTATTCGCTCCTGATTTCTTCGCCGGAATGGATCGATCATCGTCGGAAAAGCTCATCCTCGTCAACCGGGAACCAAACGGGATCGCATTCGTTACGATCAACCGACCGAAGTCCTTGAATTCGTTAACGAGAGCGATGATGGCAGATTTGGCTCAGGCGTTTAAGAGCTTGGACCGCGACGAGTCCGTGCGAGTTATCGTGCTCTCTGGAACAGGCCGAGCCTTCTGTTCCGGTGTAGACCTTACTGCGGCGGAGGATGTTTTCAAAGGAGATGTGAAGGATGTTGAAGCCGATCCGGTTGTGCAAATGGAACGCTGCCGGAAGCCGATAATCGGAGCGGTTGCAGGTTTCGCCATCACCGCTGGATTTGAGATCGCCTTGGCTTGCGACATACTCATAGCTGCCAAAGGAGCTAAGTTCATCGATACTCATGCTAGATTTCGGATATTTCCTTCATGGGGATTGTCTCAGAAACTCCCGAGGATCATCGGAGTTAACAAAGCTCGTGAAGTATCCTTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGAGGAAGGGTTTGGTGAATCACGTGGTCGACGGAGGTGAATTGTTGAAGAAAACTCGAGAGATTGCGGAGGCCATCTTGAAGAACAATCAGGACTTGGTGTTGAGATACAAGTCCGTCATTAACGATGGCCTGAAGCTGGATCTTGGCCATGCTCTTGCGCTGGAGAAGGAGAGAGCTCATGCATATTACAATGGAATGACCAAAGAGCAATTCAAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAGCCTTCCAAGTTATAGTCTAATCGATGTAGCTTACTGTCTGTTTCTACACTGCAACAATAATAAGAAGAACTTGCCTCCTAAACGGTTAGTGGATTCTTCCCTTGTTTTATTCACATGTAATTTGATTTTAGTGGATTAGAGATAAAATGACTATTTTGAGAAGAATTCAGGCTGGTTTCTGAGCAATAAATGTTGGGTATTTTATGGATAATGGCTGAATATGAACTCATTGGATGGCAGTTTATGGTTGATTTGATATTACATTGTAAGGGTAGATTATAAATAACAATTATTTGTAAAGGAAGAATCCACAAGACATTCAGAACAGGATATATAAATGTTTAACATGACCTTCCCCCACCCAACTGAGTGGTGATGGTTCATGAACACAGTTAAGAGAGAGGTAAAATGTGAAGTCCTCACGTCGGTTAGAGAGAGGAACGAAAGGGTGTGGAAATCTTTTCCTAGTAGATGTGTTTTAAAACTTTGAGAACAAGCTTGAAAGGGAAAATCCAAAAAGAACAATATTTGTTAGTGGTGTGGCATGAGCTGTTAGAAATGGTGTCAGAGCAAGACATCGGGGGTGTGTCAACGAAGACGTTGGGTTCCTATGGAGATGGATTGTGAGATCCCACATCCGTTGGCCAGACACTGAGCAGTGTCAAACCAAAATACCCAACAATCGCCAGGTTGTCTCTTACCTGGCCATGGATTCAGCAGCAGTTTGAAGAGGGGAACAAAAC

Coding sequence (CDS)

ATGGATCGATCATCGTCGGAAAAGCTCATCCTCGTCAACCGGGAACCAAACGGGATCGCATTCGTTACGATCAACCGACCGAAGTCCTTGAATTCGTTAACGAGAGCGATGATGGCAGATTTGGCTCAGGCGTTTAAGAGCTTGGACCGCGACGAGTCCGTGCGAGTTATCGTGCTCTCTGGAACAGGCCGAGCCTTCTGTTCCGGTGTAGACCTTACTGCGGCGGAGGATGTTTTCAAAGGAGATGTGAAGGATGTTGAAGCCGATCCGGTTGTGCAAATGGAACGCTGCCGGAAGCCGATAATCGGAGCGGTTGCAGGTTTCGCCATCACCGCTGGATTTGAGATCGCCTTGGCTTGCGACATACTCATAGCTGCCAAAGGAGCTAAGTTCATCGATACTCATGCTAGATTTCGGATATTTCCTTCATGGGGATTGTCTCAGAAACTCCCGAGGATCATCGGAGTTAACAAAGCTCGTGAAGTATCCTTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGAGGAAGGGTTTGGTGAATCACGTGGTCGACGGAGGTGAATTGTTGAAGAAAACTCGAGAGATTGCGGAGGCCATCTTGAAGAACAATCAGGACTTGGTGTTGAGATACAAGTCCGTCATTAACGATGGCCTGAAGCTGGATCTTGGCCATGCTCTTGCGCTGGAGAAGGAGAGAGCTCATGCATATTACAATGGAATGACCAAAGAGCAATTCAAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAGCCTTCCAAGTTATAG

Protein sequence

MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIAGRSSKKPSKL
BLAST of CmaCh17G003710 vs. Swiss-Prot
Match: ECH1P_ARATH (Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana GN=ECHIA PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.1e-112
Identity = 205/262 (78.24%), Postives = 231/262 (88.17%), Query Frame = 1

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD++ SE LI V +E  GIA +TINRPKSLNSLTRAMM DLA+AFK +D DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GR+FCSGVDLTAAE VFKGDVKD E DPVVQMER RKPIIGA+ GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DIL+A++GAKF+DTHARF IFPSWGLSQKL RIIG NKAREVSLT+MPLTA+ A + G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVV+ GE LKK REIAEAI+KN Q +VLR KSVINDGLKLDLGHAL LEKERAHAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

BLAST of CmaCh17G003710 vs. Swiss-Prot
Match: CRT_CLOAB (Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) GN=crt PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.1e-31
Identity = 85/242 (35.12%), Postives = 137/242 (56.61%), Query Frame = 1

Query: 19  IAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTG-RAFCSGVDLTAAED 78
           +A VTINRPK+LN+L    + ++      ++ D  V  ++L+G G ++F +G D++  ++
Sbjct: 14  VAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSFVAGADISEMKE 73

Query: 79  V--FKGDVKDVEADPVVQ-MERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFIDT 138
           +   +G    +  + V + +E   KP+I AV GFA+  G EIA++CDI IA+  A+F   
Sbjct: 74  MNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIRIASSNARFGQP 133

Query: 139 HARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTR 198
                I P +G +Q+L R++G+  A+++  TA  + A+EA R GLVN VV+  EL+   +
Sbjct: 134 EVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKVVEPSELMNTAK 193

Query: 199 EIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIA 257
           EIA  I+ N    V   K  IN G++ D+  ALA E E     ++  T++Q   M  FI 
Sbjct: 194 EIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFS--TEDQKDAMTAFIE 253

BLAST of CmaCh17G003710 vs. Swiss-Prot
Match: ECHM_DICDI (Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum GN=echs1 PE=3 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 6.1e-31
Identity = 86/242 (35.54%), Postives = 134/242 (55.37%), Query Frame = 1

Query: 19  IAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGRAFCSGVDLTAAEDV 78
           +  VT+NRPKSLN+L+  +++++  A K    D+ V  I+++G+ +AF +G D+   E V
Sbjct: 34  VGLVTLNRPKSLNALSDGLISEINSAVKLFQEDKDVGSIIITGSEKAFAAGADIKEMEKV 93

Query: 79  FKGDVKDVEADPVVQ---MERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFIDTH 138
              D  +   D + Q   + + RKPII AV G+A+  G E+A+ CDI+IA + A F    
Sbjct: 94  TLPDAYN--NDLLAQWHDLTKIRKPIIAAVNGYALGGGCELAMMCDIIIAGEKAVFGQPE 153

Query: 139 ARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTRE 198
            +    P  G +Q+L R IG +KA E+ LT   LTA EAE+ GLV+ VV   ELL +  +
Sbjct: 154 IKLGTIPGCGGTQRLIRAIGKSKAMELVLTGNNLTAVEAEKAGLVSKVVPVEELLTEATK 213

Query: 199 IAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIAG 258
           +AE I   +Q  V   K  +N   +L L   +  E+   H+ +   T +Q + M  F+  
Sbjct: 214 MAEKIASYSQLTVAMAKEAVNASYELTLQEGIRFERRMFHSTFG--THDQKEGMNAFVEK 271

BLAST of CmaCh17G003710 vs. Swiss-Prot
Match: HPCD_METS5 (3-hydroxypropionyl-coenzyme A dehydratase OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348) GN=Msed_2001 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.0e-30
Identity = 78/222 (35.14%), Postives = 127/222 (57.21%), Query Frame = 1

Query: 14  REPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGRAFCSGVDLT 73
           ++   + ++T+NRP  LN+L   ++ +L +A    + D  +RVI+++G G+AFC+G D+T
Sbjct: 9   KKEGNLFWITLNRPDKLNALNAKLLEELDRAVSQAESDPEIRVIIITGKGKAFCAGADIT 68

Query: 74  -----AAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKG 133
                   + +K   K  E   + ++E   KP I  + G+A+  G E+ALACDI IAA+ 
Sbjct: 69  QFNQLTPAEAWKFSKKGREI--MDKIEALSKPTIAMINGYALGGGLELALACDIRIAAEE 128

Query: 134 AKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGE 193
           A+         I+P +G +Q+L R+IG  +A E+ +T   +  ++AE+ GLVN VV    
Sbjct: 129 AQLGLPEINLGIYPGYGGTQRLTRVIGKGRALEMMMTGDRIPGKDAEKYGLVNRVVPLAN 188

Query: 194 LLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALE 231
           L ++TR++AE I K +   +   K V+N GL   L   LALE
Sbjct: 189 LEQETRKLAEKIAKKSPISLALIKEVVNRGLDSPLLSGLALE 228

BLAST of CmaCh17G003710 vs. Swiss-Prot
Match: ECHA8_MYCBO (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) GN=echA8 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.8e-30
Identity = 83/249 (33.33%), Postives = 130/249 (52.21%), Query Frame = 1

Query: 10  ILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGRAFCSG 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD D  +  I+++G+ +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVEADPVV-QMERCRKPIIGAVAGFAITAGFEIALACDILIAAKG 129
            D+    D+   D    +      ++   R P I AVAG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGE 189
           AKF     +  + P  G SQ+L R IG  KA ++ LT   + A EAER GLV+ VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKK 249
           LL + R  A  I + +       K  +N   +  L   L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

BLAST of CmaCh17G003710 vs. TrEMBL
Match: A0A0A0KCM0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G330970 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 1.9e-127
Identity = 237/264 (89.77%), Postives = 251/264 (95.08%), Query Frame = 1

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSSE LILVNRE NGIAF+TINRPKSLNSLT+ MMADLA AFKSLDRD+SVRVI+LS
Sbjct: 1   MDRSSSENLILVNRESNGIAFITINRPKSLNSLTKHMMADLAHAFKSLDRDDSVRVIILS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           N VV+G ELLKK RE+AEAILKNNQDLV+RYKSVINDGLKLDLG AL LEKERAHAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVVRYKSVINDGLKLDLGQALTLEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

BLAST of CmaCh17G003710 vs. TrEMBL
Match: D7SYK1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0077g00860 PE=4 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 5.8e-121
Identity = 222/259 (85.71%), Postives = 241/259 (93.05%), Query Frame = 1

Query: 4   SSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTG 63
           SSSE LILVNREP+GIAFVTINRP+ LNSLTR MM DLA+AFKSLD D+ VRVIV++G+G
Sbjct: 5   SSSEGLILVNREPSGIAFVTINRPQLLNSLTRPMMVDLARAFKSLDADDLVRVIVITGSG 64

Query: 64  RAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALACDIL 123
           RAFCSGVDLTAAE+VFKGDVKDVE DPVVQMERCRKPI+GA+ GFA+TAGFEIALACDIL
Sbjct: 65  RAFCSGVDLTAAEEVFKGDVKDVEIDPVVQMERCRKPIVGAIGGFAVTAGFEIALACDIL 124

Query: 124 IAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHV 183
           +A KG KFIDTHARF IFPSWGLSQKL RIIG N+AREVSLTAMP+TAE+AER G VNHV
Sbjct: 125 VAVKGTKFIDTHARFGIFPSWGLSQKLSRIIGANRAREVSLTAMPVTAEQAERWGFVNHV 184

Query: 184 VDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTK 243
           V+G ELLKK RE+AEAI+KNNQDLV+RYKSVINDGLKLDLGHALALEKERAH YYNGMTK
Sbjct: 185 VEGSELLKKAREVAEAIIKNNQDLVMRYKSVINDGLKLDLGHALALEKERAHNYYNGMTK 244

Query: 244 EQFKKMQEFIAGRSSKKPS 263
           EQFKKMQEFIAGRSSKKPS
Sbjct: 245 EQFKKMQEFIAGRSSKKPS 263

BLAST of CmaCh17G003710 vs. TrEMBL
Match: G7JNX7_MEDTR (Enoyl-CoA hydratase/isomerase family protein OS=Medicago truncatula GN=MTR_4g122220 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 2.9e-120
Identity = 224/260 (86.15%), Postives = 242/260 (93.08%), Query Frame = 1

Query: 5   SSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGR 64
           SS+KLILVNREP GIA VTINRP SLNSLTRAM  DLAQAFKSLDRDESVRVI+L+GTGR
Sbjct: 2   SSQKLILVNREPKGIAVVTINRPDSLNSLTRAMNVDLAQAFKSLDRDESVRVIILTGTGR 61

Query: 65  AFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALACDILI 124
           +FCSGVDLTAAEDVFKGDVKD E+DPVVQMERC KPIIGA+ GFA+TAGFEIALACDIL+
Sbjct: 62  SFCSGVDLTAAEDVFKGDVKDPESDPVVQMERCHKPIIGAIKGFAVTAGFEIALACDILV 121

Query: 125 AAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVV 184
           AAKGAKF+DTHARF IFPSWGLSQKL RIIGVNKAREVSLTA PLTAE AE+ GLVNH+V
Sbjct: 122 AAKGAKFMDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTATPLTAEVAEKLGLVNHLV 181

Query: 185 DGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKE 244
           + GEL+KK+REIAEAI KN+QD+VLRYKSVINDG+KLDLG AL+LEKER HAYYNGMTKE
Sbjct: 182 EDGELMKKSREIAEAIAKNSQDMVLRYKSVINDGIKLDLGRALSLEKERGHAYYNGMTKE 241

Query: 245 QFKKMQEFIAGRSSKKPSKL 265
           QFKKMQEFIAGRSSKK SKL
Sbjct: 242 QFKKMQEFIAGRSSKKQSKL 261

BLAST of CmaCh17G003710 vs. TrEMBL
Match: Q9SMK7_CICAR (Putative enoyl CoA hydratase OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 3.8e-120
Identity = 220/264 (83.33%), Postives = 245/264 (92.80%), Query Frame = 1

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD+SS +KLILVNR+PNGIA VTINRP SLNSLTRAM  DLAQAFKSLDRDE+VRVI+L+
Sbjct: 1   MDQSSPQKLILVNRDPNGIATVTINRPDSLNSLTRAMNVDLAQAFKSLDRDEAVRVIILT 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GR+FCSGVDLTAAED+FKGDVKD E+DPVVQMERCRKPIIGA+ GFA+TAGFEIALAC
Sbjct: 61  GSGRSFCSGVDLTAAEDIFKGDVKDPESDPVVQMERCRKPIIGAIRGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DI++A KG+KF+DTHARF IFPSWGLSQKL RIIGVNKAREVSLTA PLTAE A++ G V
Sbjct: 121 DIVVAVKGSKFMDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTATPLTAEVADKLGFV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NH+V+ GELLKK+REIAEAI+KNNQD+VLRYKSVINDG+KLDLGHAL+LEKER H YY G
Sbjct: 181 NHLVEDGELLKKSREIAEAIVKNNQDMVLRYKSVINDGIKLDLGHALSLEKERGHDYYKG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKK SKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKQSKL 264

BLAST of CmaCh17G003710 vs. TrEMBL
Match: B0M193_SOYBN (Peroxisomal enoyl-CoA hydratase/isomerase family protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 6.4e-120
Identity = 222/264 (84.09%), Postives = 242/264 (91.67%), Query Frame = 1

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD  S EKLILVNREPNG+A VTINRP SLNSLTR MM DLAQAFK LDRDE VRVI+L+
Sbjct: 15  MDGKSPEKLILVNREPNGVALVTINRPGSLNSLTRPMMVDLAQAFKRLDRDELVRVIILT 74

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GR+FCSGVDLTAAEDVFKGDVKD E+DPVVQME CRKPIIGA+ GFA+TAGFEIALAC
Sbjct: 75  GSGRSFCSGVDLTAAEDVFKGDVKDPESDPVVQMELCRKPIIGAIRGFAVTAGFEIALAC 134

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DIL+AAKG+KF+DTHARF IFPSWGLSQKL R+IG NKAREVSL+A PLTAE AE+ G V
Sbjct: 135 DILVAAKGSKFMDTHARFGIFPSWGLSQKLSRVIGANKAREVSLSATPLTAEVAEKLGFV 194

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVV+ GELLKK+REIA+AI+KNNQDLVLRYK+VINDGLKLDLGHAL+LEKERAH YYNG
Sbjct: 195 NHVVEEGELLKKSREIADAIVKNNQDLVLRYKAVINDGLKLDLGHALSLEKERAHNYYNG 254

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKK SKL
Sbjct: 255 MTKEQFKKMQEFIAGRSSKKQSKL 278

BLAST of CmaCh17G003710 vs. TAIR10
Match: AT4G16210.1 (AT4G16210.1 enoyl-CoA hydratase/isomerase A)

HSP 1 Score: 407.5 bits (1046), Expect = 6.2e-114
Identity = 205/262 (78.24%), Postives = 231/262 (88.17%), Query Frame = 1

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD++ SE LI V +E  GIA +TINRPKSLNSLTRAMM DLA+AFK +D DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GR+FCSGVDLTAAE VFKGDVKD E DPVVQMER RKPIIGA+ GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DIL+A++GAKF+DTHARF IFPSWGLSQKL RIIG NKAREVSLT+MPLTA+ A + G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVV+ GE LKK REIAEAI+KN Q +VLR KSVINDGLKLDLGHAL LEKERAHAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

BLAST of CmaCh17G003710 vs. TAIR10
Match: AT1G60550.1 (AT1G60550.1 enoyl-CoA hydratase/isomerase D)

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-25
Identity = 79/214 (36.92%), Postives = 112/214 (52.34%), Query Frame = 1

Query: 18  GIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTG-RAFCSGVDLTAAE 77
           GIA +TINRP+  N+     + +L +AF     D SV VI+L+G G +AFCSG D     
Sbjct: 86  GIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAFCSGGDQALRT 145

Query: 78  DVFKGDVKDVEA----DPVVQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFI 137
                D  DV      D  VQ+ R  KP+I  VAG+A+  G  + + CD+ IAA  A F 
Sbjct: 146 QDGYADPNDVGRLNVLDLQVQIRRLPKPVIAMVAGYAVGGGHILHMVCDLTIAADNAIFG 205

Query: 138 DTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKK 197
            T  +   F +   S  + R++G  KARE+       TA EAE+ GL+N VV   +L K+
Sbjct: 206 QTGPKVGSFDAGYGSSIMSRLVGPKKAREMWFMTRFYTASEAEKMGLINTVVPLEDLEKE 265

Query: 198 TREIAEAILKNNQDLVLRYKSVINDGLKLDLGHA 227
           T +    IL+N+   +   K+ +N    +D GHA
Sbjct: 266 TVKWCREILRNSPTAIRVLKAALN---AVDDGHA 296

BLAST of CmaCh17G003710 vs. TAIR10
Match: AT5G43280.1 (AT5G43280.1 delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1)

HSP 1 Score: 106.7 bits (265), Expect = 2.2e-23
Identity = 70/218 (32.11%), Postives = 107/218 (49.08%), Query Frame = 1

Query: 1   MDRSSSEKLILVNREPNGIAF-VTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVL 60
           M   S + L ++ +  +   F + INRP  LN+L+     +  +A  SLD++  V VI+L
Sbjct: 1   MTMESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL 60

Query: 61  SGTGRAFCSGVDLTAAEDV----------------FKGDVKDVEADPVVQMERCRKPIIG 120
           SG G+ FCSG+DL +   +                 +  +K ++A  +  +E+CRKP+I 
Sbjct: 61  SGAGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIA 120

Query: 121 AVAGFAITAGFEIALACDILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVS 180
           A+ G  I  G ++  ACDI   ++ A F        I    G  Q+LP I+G   A E++
Sbjct: 121 AIHGACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELA 180

Query: 181 LTAMPLTAEEAERKGLVNHVVDG-GELLKKTREIAEAI 201
           LTA   +  EA+  GLV+ V     EL      IAE I
Sbjct: 181 LTARRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGI 217

BLAST of CmaCh17G003710 vs. TAIR10
Match: AT4G16800.1 (AT4G16800.1 ATP-dependent caseinolytic (Clp) protease/crotonase family protein)

HSP 1 Score: 105.5 bits (262), Expect = 5.0e-23
Identity = 69/228 (30.26%), Postives = 116/228 (50.88%), Query Frame = 1

Query: 8   KLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVL-SGTGRAF 67
           KL  ++   +GI  V ++RP + N++ + M+  L  AF+S+ +D S RV+++ S     F
Sbjct: 44  KLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIRSLVPGVF 103

Query: 68  CSGVDLTAAEDVFKGDVKDVEADPVVQ---MERCRKPIIGAVAGFAITAGFEIALACDIL 127
           C+G DL     +   +V             +E    P I A+ G A+  G E+ALACD+ 
Sbjct: 104 CAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALGGGLEMALACDLR 163

Query: 128 IAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHV 187
           I  + A F        I P  G +Q+L R++G + ++E+  T   + A EA  KGLVN  
Sbjct: 164 ICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDAIEAANKGLVNIC 223

Query: 188 VDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEK 232
           V  GE  +K  E+A+ I +     +   K  I++G++ ++   L +E+
Sbjct: 224 VTAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVEE 271

BLAST of CmaCh17G003710 vs. TAIR10
Match: AT4G29010.1 (AT4G29010.1 Enoyl-CoA hydratase/isomerase family)

HSP 1 Score: 102.4 bits (254), Expect = 4.2e-22
Identity = 60/186 (32.26%), Postives = 106/186 (56.99%), Query Frame = 1

Query: 17  NGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGRAFCSGVDLTAAE 76
           +G+A +TI+ P  +NSL   +++ L + F+  ++   V+ IVL G    F  G D+   +
Sbjct: 14  DGVAVITISNPP-VNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGRFSGGFDINVFQ 73

Query: 77  DVFK-GDVK---DVEADPVVQ-MERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKF 136
            V K GD+    +V  + V   ME  RKP++ AV G A+  G E+A+AC   +AA  A+ 
Sbjct: 74  QVHKTGDLSLMPEVSVELVCNLMEDSRKPVVAAVEGLALGGGLELAMACHARVAAPKAQL 133

Query: 137 IDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLK 196
                   + P +G +Q+LPR++G+ KA ++ L +  +++EE  + GL++ +V  G++L 
Sbjct: 134 GLPELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISSEEGHKLGLIDALVPPGDVLS 193

Query: 197 KTREIA 198
            +R+ A
Sbjct: 194 TSRKWA 198

BLAST of CmaCh17G003710 vs. NCBI nr
Match: gi|659116964|ref|XP_008458352.1| (PREDICTED: probable enoyl-CoA hydratase, mitochondrial [Cucumis melo])

HSP 1 Score: 464.2 bits (1193), Expect = 1.6e-127
Identity = 238/264 (90.15%), Postives = 251/264 (95.08%), Query Frame = 1

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS E LILVNRE NGIAF+TINRPKSLNSLTR MM DLA+AFKSLDRD+SVRVI+LS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           N VV+G ELLKK RE+AEAILKNNQDLVLRYKSVINDGLKLDLG ALALEKERAHAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

BLAST of CmaCh17G003710 vs. NCBI nr
Match: gi|449462144|ref|XP_004148801.1| (PREDICTED: probable enoyl-CoA hydratase, mitochondrial [Cucumis sativus])

HSP 1 Score: 463.4 bits (1191), Expect = 2.7e-127
Identity = 237/264 (89.77%), Postives = 251/264 (95.08%), Query Frame = 1

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSSE LILVNRE NGIAF+TINRPKSLNSLT+ MMADLA AFKSLDRD+SVRVI+LS
Sbjct: 1   MDRSSSENLILVNRESNGIAFITINRPKSLNSLTKHMMADLAHAFKSLDRDDSVRVIILS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           N VV+G ELLKK RE+AEAILKNNQDLV+RYKSVINDGLKLDLG AL LEKERAHAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVVRYKSVINDGLKLDLGQALTLEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

BLAST of CmaCh17G003710 vs. NCBI nr
Match: gi|225431978|ref|XP_002278726.1| (PREDICTED: probable enoyl-CoA hydratase, mitochondrial [Vitis vinifera])

HSP 1 Score: 441.8 bits (1135), Expect = 8.4e-121
Identity = 222/259 (85.71%), Postives = 241/259 (93.05%), Query Frame = 1

Query: 4   SSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTG 63
           SSSE LILVNREP+GIAFVTINRP+ LNSLTR MM DLA+AFKSLD D+ VRVIV++G+G
Sbjct: 5   SSSEGLILVNREPSGIAFVTINRPQLLNSLTRPMMVDLARAFKSLDADDLVRVIVITGSG 64

Query: 64  RAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALACDIL 123
           RAFCSGVDLTAAE+VFKGDVKDVE DPVVQMERCRKPI+GA+ GFA+TAGFEIALACDIL
Sbjct: 65  RAFCSGVDLTAAEEVFKGDVKDVEIDPVVQMERCRKPIVGAIGGFAVTAGFEIALACDIL 124

Query: 124 IAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHV 183
           +A KG KFIDTHARF IFPSWGLSQKL RIIG N+AREVSLTAMP+TAE+AER G VNHV
Sbjct: 125 VAVKGTKFIDTHARFGIFPSWGLSQKLSRIIGANRAREVSLTAMPVTAEQAERWGFVNHV 184

Query: 184 VDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTK 243
           V+G ELLKK RE+AEAI+KNNQDLV+RYKSVINDGLKLDLGHALALEKERAH YYNGMTK
Sbjct: 185 VEGSELLKKAREVAEAIIKNNQDLVMRYKSVINDGLKLDLGHALALEKERAHNYYNGMTK 244

Query: 244 EQFKKMQEFIAGRSSKKPS 263
           EQFKKMQEFIAGRSSKKPS
Sbjct: 245 EQFKKMQEFIAGRSSKKPS 263

BLAST of CmaCh17G003710 vs. NCBI nr
Match: gi|951002388|ref|XP_014507320.1| (PREDICTED: short-chain-enoyl-CoA hydratase [Vigna radiata var. radiata])

HSP 1 Score: 440.3 bits (1131), Expect = 2.4e-120
Identity = 223/264 (84.47%), Postives = 243/264 (92.05%), Query Frame = 1

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDR S EKLILVNR PNG+A +TINRP SLNSLTR MM DLAQAFK LDRDESVRVI+L+
Sbjct: 1   MDRQSPEKLILVNRHPNGVALITINRPGSLNSLTRPMMVDLAQAFKKLDRDESVRVIILT 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GR+FCSGVDLTAAEDVFKGDVKD E+DPVVQME CRKPIIGAV GFA+TAGFEIALAC
Sbjct: 61  GSGRSFCSGVDLTAAEDVFKGDVKDPESDPVVQMELCRKPIIGAVRGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DIL+AAKG+KF+DTHARF IFPSWGLSQKL RIIGVNKAREVSLTA PLTAE AE+ G V
Sbjct: 121 DILVAAKGSKFMDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTASPLTAEVAEKLGFV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVV+ GELLKK+REIA+AI+KNNQDLVLRYK+VINDG+KLDLG AL+LEKERAH YYNG
Sbjct: 181 NHVVEEGELLKKSREIADAIVKNNQDLVLRYKAVINDGIKLDLGRALSLEKERAHDYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSKK SKL
Sbjct: 241 MTKEQFRKMQEFIAGRSSKKQSKL 264

BLAST of CmaCh17G003710 vs. NCBI nr
Match: gi|357479003|ref|XP_003609787.1| (enoyl-CoA hydratase/isomerase family protein [Medicago truncatula])

HSP 1 Score: 439.5 bits (1129), Expect = 4.1e-120
Identity = 224/260 (86.15%), Postives = 242/260 (93.08%), Query Frame = 1

Query: 5   SSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGR 64
           SS+KLILVNREP GIA VTINRP SLNSLTRAM  DLAQAFKSLDRDESVRVI+L+GTGR
Sbjct: 2   SSQKLILVNREPKGIAVVTINRPDSLNSLTRAMNVDLAQAFKSLDRDESVRVIILTGTGR 61

Query: 65  AFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALACDILI 124
           +FCSGVDLTAAEDVFKGDVKD E+DPVVQMERC KPIIGA+ GFA+TAGFEIALACDIL+
Sbjct: 62  SFCSGVDLTAAEDVFKGDVKDPESDPVVQMERCHKPIIGAIKGFAVTAGFEIALACDILV 121

Query: 125 AAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVV 184
           AAKGAKF+DTHARF IFPSWGLSQKL RIIGVNKAREVSLTA PLTAE AE+ GLVNH+V
Sbjct: 122 AAKGAKFMDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTATPLTAEVAEKLGLVNHLV 181

Query: 185 DGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKE 244
           + GEL+KK+REIAEAI KN+QD+VLRYKSVINDG+KLDLG AL+LEKER HAYYNGMTKE
Sbjct: 182 EDGELMKKSREIAEAIAKNSQDMVLRYKSVINDGIKLDLGRALSLEKERGHAYYNGMTKE 241

Query: 245 QFKKMQEFIAGRSSKKPSKL 265
           QFKKMQEFIAGRSSKK SKL
Sbjct: 242 QFKKMQEFIAGRSSKKQSKL 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ECH1P_ARATH1.1e-11278.24Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana GN=ECHIA PE=... [more]
CRT_CLOAB2.1e-3135.12Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 /... [more]
ECHM_DICDI6.1e-3135.54Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum GN=echs1... [more]
HPCD_METS51.0e-3035.143-hydroxypropionyl-coenzyme A dehydratase OS=Metallosphaera sedula (strain ATCC ... [more]
ECHA8_MYCBO1.8e-3033.33Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 /... [more]
Match NameE-valueIdentityDescription
A0A0A0KCM0_CUCSA1.9e-12789.77Uncharacterized protein OS=Cucumis sativus GN=Csa_6G330970 PE=4 SV=1[more]
D7SYK1_VITVI5.8e-12185.71Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0077g00860 PE=4 SV=... [more]
G7JNX7_MEDTR2.9e-12086.15Enoyl-CoA hydratase/isomerase family protein OS=Medicago truncatula GN=MTR_4g122... [more]
Q9SMK7_CICAR3.8e-12083.33Putative enoyl CoA hydratase OS=Cicer arietinum PE=2 SV=1[more]
B0M193_SOYBN6.4e-12084.09Peroxisomal enoyl-CoA hydratase/isomerase family protein OS=Glycine max PE=2 SV=... [more]
Match NameE-valueIdentityDescription
AT4G16210.16.2e-11478.24 enoyl-CoA hydratase/isomerase A[more]
AT1G60550.11.1e-2536.92 enoyl-CoA hydratase/isomerase D[more]
AT5G43280.12.2e-2332.11 delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1[more]
AT4G16800.15.0e-2330.26 ATP-dependent caseinolytic (Clp) protease/crotonase family protein[more]
AT4G29010.14.2e-2232.26 Enoyl-CoA hydratase/isomerase family[more]
Match NameE-valueIdentityDescription
gi|659116964|ref|XP_008458352.1|1.6e-12790.15PREDICTED: probable enoyl-CoA hydratase, mitochondrial [Cucumis melo][more]
gi|449462144|ref|XP_004148801.1|2.7e-12789.77PREDICTED: probable enoyl-CoA hydratase, mitochondrial [Cucumis sativus][more]
gi|225431978|ref|XP_002278726.1|8.4e-12185.71PREDICTED: probable enoyl-CoA hydratase, mitochondrial [Vitis vinifera][more]
gi|951002388|ref|XP_014507320.1|2.4e-12084.47PREDICTED: short-chain-enoyl-CoA hydratase [Vigna radiata var. radiata][more]
gi|357479003|ref|XP_003609787.1|4.1e-12086.15enoyl-CoA hydratase/isomerase family protein [Medicago truncatula][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001753Enoyl-CoA_hydra/iso
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018874 benzoate metabolic process
biological_process GO:0019482 beta-alanine metabolic process
biological_process GO:0006635 fatty acid beta-oxidation
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006550 isoleucine catabolic process
biological_process GO:0006552 leucine catabolic process
biological_process GO:0046251 limonene catabolic process
biological_process GO:0006554 lysine catabolic process
biological_process GO:0006568 tryptophan metabolic process
biological_process GO:0006574 valine catabolic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005777 peroxisome
cellular_component GO:0005575 cellular_component
molecular_function GO:0004300 enoyl-CoA hydratase activity
molecular_function GO:0016853 isomerase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G003710.1CmaCh17G003710.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001753Crotonase superfamilyPFAMPF00378ECH_1coord: 15..251
score: 4.8
NoneNo IPR availablePANTHERPTHR11941ENOYL-COA HYDRATASE-RELATEDcoord: 1..260
score: 5.6E
NoneNo IPR availablePANTHERPTHR11941:SF72SUBFAMILY NOT NAMEDcoord: 1..260
score: 5.6E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh17G003710CmaCh08G012800Cucurbita maxima (Rimu)cmacmaB373
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh17G003710Cucurbita maxima (Rimu)cmacmaB246
CmaCh17G003710Cucurbita maxima (Rimu)cmacmaB249
CmaCh17G003710Cucurbita maxima (Rimu)cmacmaB367
CmaCh17G003710Cucurbita maxima (Rimu)cmacmaB369
CmaCh17G003710Cucumber (Gy14) v1cgycmaB0183
CmaCh17G003710Cucumber (Gy14) v1cgycmaB0714
CmaCh17G003710Cucurbita moschata (Rifu)cmacmoB350
CmaCh17G003710Cucurbita moschata (Rifu)cmacmoB363
CmaCh17G003710Wild cucumber (PI 183967)cmacpiB357
CmaCh17G003710Wild cucumber (PI 183967)cmacpiB359
CmaCh17G003710Cucumber (Chinese Long) v2cmacuB353
CmaCh17G003710Cucumber (Chinese Long) v2cmacuB355
CmaCh17G003710Melon (DHL92) v3.5.1cmameB342
CmaCh17G003710Watermelon (Charleston Gray)cmawcgB320
CmaCh17G003710Watermelon (Charleston Gray)cmawcgB313
CmaCh17G003710Watermelon (Charleston Gray)cmawcgB317
CmaCh17G003710Watermelon (97103) v1cmawmB335
CmaCh17G003710Watermelon (97103) v1cmawmB344
CmaCh17G003710Watermelon (97103) v1cmawmB348
CmaCh17G003710Cucurbita pepo (Zucchini)cmacpeB403
CmaCh17G003710Cucurbita pepo (Zucchini)cmacpeB412
CmaCh17G003710Bottle gourd (USVL1VR-Ls)cmalsiB344
CmaCh17G003710Bottle gourd (USVL1VR-Ls)cmalsiB350
CmaCh17G003710Cucumber (Gy14) v2cgybcmaB345
CmaCh17G003710Melon (DHL92) v3.6.1cmamedB398
CmaCh17G003710Silver-seed gourdcarcmaB0392
CmaCh17G003710Cucumber (Chinese Long) v3cmacucB0421
CmaCh17G003710Cucumber (Chinese Long) v3cmacucB0425
CmaCh17G003710Watermelon (97103) v2cmawmbB359
CmaCh17G003710Watermelon (97103) v2cmawmbB363
CmaCh17G003710Watermelon (97103) v2cmawmbB366
CmaCh17G003710Wax gourdcmawgoB0453
CmaCh17G003710Wax gourdcmawgoB0470
CmaCh17G003710Wax gourdcmawgoB0473