CmaCh16G011140 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G011140
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionFatty acid oxidation complex subunit alpha
LocationCma_Chr16 : 8569600 .. 8574739 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGTTTTGTATTAAGTATGGGGGCATTCAGAGTTCTGTGCGGAAAGCTGATCAATCTCAGAACCCAACTCCATCATTCTCCTCAGAACCTTTCGATTCCGGCCACCCTGCCCTTCCGTACATTTCCATGGCGGTACACTCATTGCAGAACTCTCATTTTGGAGTCGGTGTTTCCTGAGTCTGTCCGTCTTCACCGACTTTCAGACTCCGATTCTGGTAAGTTCTGAGCTGAGGATATGTATGTCTTTTTTTTTTAATTTTGTTGTGTGATATGCTTTAATAGTCTGCGATCAATGGTTATATGCTTGGGTTATTTAGATTGCTGAAGTTTAAGGATTTTGTTGTGATGTCTCAGTTGGATCCTGAATTTGCTAGTTTAAGATTTGGTGCTTAAGTAATAAGTAATATTCCCTTGCATGTCCGTTTGAGAGGTGCTGAATGTCACGGTCGGGCGTGTTGCCCATGCCAAGCTATTGTATTTTTTATTTGTATGACTGTTTGGCGAAATCATGTCTAGGCATGCTAGACTTGAATAACCCCAAAACCTGAATTACCCAAAATGTACTATAGGTGTGACTAGTTGTCAACTTCTCCCTTGAAATCACCTCTAGGCCTGTTAGATCTGAATCGCCTCAAAATGTATTATAGGGGGTGTGACTAGTTGCCAACTCCTCCCTTGATTTTGTATGACCATTTGGCGAAATCACGTCTAGGCTTGCCAGACTTGAATTGCCTCAAAATGTATTATAGCGGGATGTGACTAATTGTCAAATTCTCCCTACCTGAAGAGTTATATGCTACTTAAGCCGTTGTGTTACCTTTTGCTTGTATGTCATATAACATTGCTTTCAATCTCTCATTCTTGCTTTCAAACTCTCTTTCAAAATCTCTTTGGGGTCTTCTAAAACCTTCTTCTCAATCGGGGCTAGAGGCTTGAGCATACGTTGTCCGGCTGTAAGCGACGCGAATACAAATTAACTTGACTCACTTGATGTTGGAAAGTGAGTGCCGCACAATCACACAATCGCAAAGTTGTTGCCACGATCCAACCCTTCAAAAGCTGAGGCTTCATAGTCTTTATAACATATGTCAATTGGATTTGGATTTGAGATGGAACTATATATTTATCTAATATATTATCTAATATAGTATCAGAGTCTCTGCACGTGTACCCATCACTTAAAGAGCTTAATTTTTATTCTGGAATTTATGGTTTGATTGTCTGCAGAGTATGGGAGATTTTGAGTGTGTTTGGATTAACGTCTCAAGTGATTTTGGAGGGCTAAAAATCATTTCTTCATAATTTTAGTGTTTGGTAGCGTACAAAAAGCACTTTTGAAATGATTATATTGGTTCAAAATCATTTTTTTGAAACAACACTTCAAAGTTGATAAACTTCCGATGGGTTCCTTTTTCTCATCATTTGTAGTTCTATAATAATTTCCTTTCAAGTTTCTGGAGTGTTCTTGGGCTGCTCGAGTTATATTTGAATTCTAATCCACTTGGGTTTATCAATGGCAGGTATTGTTGAGGTACATTTAGCCAGGCCTGAGGTGAAAAATGCCATTGGCAAGGATATGCTGAGGGGACTGCGTTATGCCTTTGAATCAATTGATAGCGATCCATCTGCCAATGTTATGATGATCTGCAGCTCTGTCCCAAAGGTGTTTTGTGCAGGTGCCGATTTGAAGGTATTCTTGTTTATATCTAACAATTCAACTCTCCCATTATGATATAGTCTTTAACTTCTTTTCCTCCTCAATTCATTCTTTTAAGTAAAGGAACGTAAGAAGATGACTGCATCAGAAGTTCATTCCTATGTCACTTCACTGCGGGCTGCATTCACATTTCTGGAGGTAGATTGTTATTATTATCTACAGGCGTACCTGCTTTTACATTCCATGATTTATTTACATAGTATATGAACAAGGATGTCTTGACAAGTATACAATGCATCAGTTGAGATGCACATGACTACATCAGTTATCATCGATATTCCGTCCATGATCTTTCGTGGCTGATGCTAGTAAACTTTAAGGGCTTTCTAAGTAATAAAGAATTAAAGGGTTTAGTGTGAGATCCCACATCGGTTGGGGAGGAGAATGAAACACCCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTATGGGAAGCCCAAAAGGGACAGTATTTGTATGAGCACCGGTTCCACTGTGTAACTCTGAAAGACGCGATTTAAAAACCTTGAGGGAAAGCCTGAAAGGGAAAGCCCAAAGAGGGCAATATCTGCTAGCGGTGGGCCTGGTCTGTTACATTTAGGGAGCTCAAGTCATAGTGACCACTCAAGATAGAATTTAAGATCCTACGAGTTACCTTGACAACTAAATGCAGTAAGGTTAGACGATTGTCCTGTGAGAATAGTCGAGTCAGGTACAAGTTGGCCCAAACGCTCATGGATATTTTTTTAAAACTTCCCTTTTTTCATCACAAATCTAGTTTTCAGCTGACTTAATCTAAATTTATTTTATTCAGGGACTGTGTATTCCTACTATTTCAGTTATTGAAGGAGCAGCATTCGGTAGTGGACTTGAAATGGCTCTTGCGTGTGATCTTCGGATATGTGGTAATAAATTTAGTCAATTATTTTATAGTTTATTATTTTACGTCTTGCTGTATTGGAGTTATAACTCTCATCGTTAAACCATGATCCAGGGGAAGATGCCAAGCTCAGCTTGCCAGAAACCGGACTGGCTATAATTCCTGGGTATGTAGTAAATACTTACCTATATATGCGGTCACATATGACGTTTTAAGAGCTCACGTGAGACCAGTAGAAGGAAGCCTGCATGCATATTGAAGTTCCATAGTTCTATTACTTAGTATAAACATTAAACTGTTGGTGGTGGAAGCTGGATGAGACTCTTCCCAAGACTATTTTTCAAACCCCTTTTCTATGAGAGATCCCACATTAGTTTGAGAGGAGAACGAAACATTCCTTACAAGGGTGTGGAAACCTCTCCCTAGCATATACGTTTTAAAATTGTGAGGCTGATGACGATACGTAACGGGCCAAAACGGACAATATCTTTTAGCGGTAGGCTTAAGTTGTTACAAATGGTATCAGAGCCAGACATCAGGCGGTGTGCCAGCGAGGACTCCGGGCCCCCATGGGGGGTGGATTATGAGATCTCACATTAGTTGGAGAGGAGAACGAAGCATTTCTATTAAGGGTGTGGAAACCTCTCCTAGCATATGCGTTTTAAAACCGTGAGGTCGATGGTGATACATAACGGGCCAAAGCGGACAATATCTGTTAGTGGTGCGCTTGGGCTGTTACAAATAGTATTAGAGCTAGACACCGGGTTGTGTGCCAGCGAGGACGCTAAGCCTCCAAAGGGGGTGGATTATGAGATCCCACATTGGTTGGAGAGGAGAACAAAGCATTTCTTATAAAGGTGTGGAAACCTTTCCCAAGCTGATGTGTTTTAAAACTGTGAGGCTGATAACAATACGTAACAGACCAAGGCGGACAATATTTATTTGTGGTGGACTTGGGCTGTTACATAACATGAAGGTTCTCTTGTATGCACGAGCAGATTACACCTTCTAAACATGTTTTCTCTCAGGGCAGGTGGGACTGCAAGGCTTCCCAGGCTTGTTGGGAAATCAATAGCAAAGGAACTTATATTCACTGGTCGTAAGGTCAGTGGCAGAGATGCTCTATCTATAGGTATGATTACCAATCTTTTTAAAGCTTAAGTTCAGTGTTTTATTGAGTTAAGGATAAGAAAACAAATTTCCTGCCTTAGGTCTTGTCAATTACTGTGTCCCTGCTGGTGAAGCTTATGCAAAGGCACTGGAAATTGCCCAAGAGATAAATCAGAAGGTAATTAGAACATTCTTATAATTATCTTTCTGGTTATCGTGCTGACAATCTGATGAAATCATGATCAGATTGATATGATTCCACTAGTTTGACCATATAATGCATTGAGAGATCAAAGTTGAATCATATTTTGTACCTTAATTTTCATCCATAAATTGAGAGGTGTTATAAAGGGTGAACCCACAAACTAAACACTTTGATTCCATTTGATAATAATTTTCTTTTTGTTTTTTTTTTTTTTTTTTTAAAATTATGCTTATTTCCTCGTTTATTCTTTTAAAAAATATAAGAACTACATTTCTTTTTTCAGTTTTCTATATTTGGCCGGTATTTCGACAACATTTGCAAAGTGTAGATCTAACAACCCAAACCCCCGCTAACAAATATTGTTTATTTTGTCCAATTACGTATCGACGTCAGTCTTACGATTTTAAAACGTGTCTATTAGGGAGAGGTTTTTACGCCTTTATAAGAAATGTTTCGTTTCATTCTTCAATTGATGTAGGATCTCACAATCTACCCCTTTGGGGGCTCAACCTCCTTGCTGACTCACCTATTGTGAGATCCCATATCGGTTGGAGAGGAGAACGAAACATTCTTTAAGGGTGTGGAAACATCTCCCTAACAGACGCATTTTAAAACTATGAGGCTGACTGTGATACGTAACGGGCTAGAGCGAACAATATCTATTAGCGGTGGGCTTGAGTTGTTACAAATGGTATCAGAGTCAGATACAGAGCGGTGTGCCAGCAAGGACGCTGAGCCCCCAAGAGGGGTGGACTGTGAGATCCCACATCGGTGGGAGAGGGGAACAAAACATTCTTTTTAAGAGTATGAAAACCTCTTCCTAACATACGTGTTTTAAAACCGTGAGGTTGATGGTGATACGTAACCGCTGAGCTTGAGCGGTTACACTAGATCACAAAATGAAAAAACTCGTAGTTTTACGAAACGAATGATTTTTCTCAAAACTTCTAAGATTTAGCATATGTTTTTCCTCAACTGTGTCATAGCATGCCTACAGCCTATAATCCAATACCCAAGTAATTGTTGAGTATTGTATGAGAGTAACTGTTGAGTAATGTATTAACAGGGTCCACTAGCAATAAAGATGGCAAAGAAGGCGATCGATGAGGGGCTCGAGAGAGACCTGGAATCAGCTATGGAAGTAGAAGATGAATGTTACAAAGAACTCTTAGACACGAAAGATCGCTTGGAAGGATTGACAGCATTTGCCGAGAAGCGCAAGCCGAGATATCGAGGAGAATAG

mRNA sequence

TCGTTTTGTATTAAGTATGGGGGCATTCAGAGTTCTGTGCGGAAAGCTGATCAATCTCAGAACCCAACTCCATCATTCTCCTCAGAACCTTTCGATTCCGGCCACCCTGCCCTTCCGTACATTTCCATGGCGGTACACTCATTGCAGAACTCTCATTTTGGAGTCGGTGTTTCCTGAGTCTGTCCGTCTTCACCGACTTTCAGACTCCGATTCTGGTATTGTTGAGGTACATTTAGCCAGGCCTGAGGTGAAAAATGCCATTGGCAAGGATATGCTGAGGGGACTGCGTTATGCCTTTGAATCAATTGATAGCGATCCATCTGCCAATGTTATGATGATCTGCAGCTCTGTCCCAAAGGTGTTTTGTGCAGGTGCCGATTTGAAGGAACGTAAGAAGATGACTGCATCAGAAGTTCATTCCTATGTCACTTCACTGCGGGCTGCATTCACATTTCTGGAGGGACTGTGTATTCCTACTATTTCAGTTATTGAAGGAGCAGCATTCGGTAGTGGACTTGAAATGGCTCTTGCGTGTGATCTTCGGATATGTGGGGAAGATGCCAAGCTCAGCTTGCCAGAAACCGGACTGGCTATAATTCCTGGGGCAGGTGGGACTGCAAGGCTTCCCAGGCTTGTTGGGAAATCAATAGCAAAGGAACTTATATTCACTGGTCGTAAGGTCAGTGGCAGAGATGCTCTATCTATAGGTCTTGTCAATTACTGTGTCCCTGCTGGTGAAGCTTATGCAAAGGCACTGGAAATTGCCCAAGAGATAAATCAGAAGGGTCCACTAGCAATAAAGATGGCAAAGAAGGCGATCGATGAGGGGCTCGAGAGAGACCTGGAATCAGCTATGGAAGTAGAAGATGAATGTTACAAAGAACTCTTAGACACGAAAGATCGCTTGGAAGGATTGACAGCATTTGCCGAGAAGCGCAAGCCGAGATATCGAGGAGAATAG

Coding sequence (CDS)

ATGGGGGCATTCAGAGTTCTGTGCGGAAAGCTGATCAATCTCAGAACCCAACTCCATCATTCTCCTCAGAACCTTTCGATTCCGGCCACCCTGCCCTTCCGTACATTTCCATGGCGGTACACTCATTGCAGAACTCTCATTTTGGAGTCGGTGTTTCCTGAGTCTGTCCGTCTTCACCGACTTTCAGACTCCGATTCTGGTATTGTTGAGGTACATTTAGCCAGGCCTGAGGTGAAAAATGCCATTGGCAAGGATATGCTGAGGGGACTGCGTTATGCCTTTGAATCAATTGATAGCGATCCATCTGCCAATGTTATGATGATCTGCAGCTCTGTCCCAAAGGTGTTTTGTGCAGGTGCCGATTTGAAGGAACGTAAGAAGATGACTGCATCAGAAGTTCATTCCTATGTCACTTCACTGCGGGCTGCATTCACATTTCTGGAGGGACTGTGTATTCCTACTATTTCAGTTATTGAAGGAGCAGCATTCGGTAGTGGACTTGAAATGGCTCTTGCGTGTGATCTTCGGATATGTGGGGAAGATGCCAAGCTCAGCTTGCCAGAAACCGGACTGGCTATAATTCCTGGGGCAGGTGGGACTGCAAGGCTTCCCAGGCTTGTTGGGAAATCAATAGCAAAGGAACTTATATTCACTGGTCGTAAGGTCAGTGGCAGAGATGCTCTATCTATAGGTCTTGTCAATTACTGTGTCCCTGCTGGTGAAGCTTATGCAAAGGCACTGGAAATTGCCCAAGAGATAAATCAGAAGGGTCCACTAGCAATAAAGATGGCAAAGAAGGCGATCGATGAGGGGCTCGAGAGAGACCTGGAATCAGCTATGGAAGTAGAAGATGAATGTTACAAAGAACTCTTAGACACGAAAGATCGCTTGGAAGGATTGACAGCATTTGCCGAGAAGCGCAAGCCGAGATATCGAGGAGAATAG

Protein sequence

MGAFRVLCGKLINLRTQLHHSPQNLSIPATLPFRTFPWRYTHCRTLILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVMMICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVEDECYKELLDTKDRLEGLTAFAEKRKPRYRGE
BLAST of CmaCh16G011140 vs. Swiss-Prot
Match: ECH2M_ARATH (Probable enoyl-CoA hydratase 2, mitochondrial OS=Arabidopsis thaliana GN=At4g16800 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.3e-100
Identity = 187/271 (69.00%), Postives = 224/271 (82.66%), Query Frame = 1

Query: 43  CRTLILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPS 102
           CRTLI+E+  PE V+L+RLS SDSGI+EV+L RP  KNAI K+ML+ L+ AFESI  D S
Sbjct: 30  CRTLIIETSPPEFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNS 89

Query: 103 ANVMMICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAA 162
           A V+MI S VP VFCAGADLKER+ M+ SEVH+YV SLR  F+F+E L IPTI+ IEGAA
Sbjct: 90  ARVVMIRSLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAA 149

Query: 163 FGSGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKV 222
            G GLEMALACDLRICGE+A   LPETGLAIIPGAGGT RL RLVG+S++KELIFTGRK+
Sbjct: 150 LGGGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKI 209

Query: 223 SGRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEV 282
              +A + GLVN CV AGEA+ KA+E+AQ+IN+KGPLAIKMAKKAIDEG+E ++ S +EV
Sbjct: 210 DAIEAANKGLVNICVTAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEV 269

Query: 283 EDECYKELLDTKDRLEGLTAFAEKRKPRYRG 314
           E+ CY++LL+T+DRLEGL AFAEKRKP Y G
Sbjct: 270 EEMCYQKLLNTQDRLEGLAAFAEKRKPLYTG 300

BLAST of CmaCh16G011140 vs. Swiss-Prot
Match: AUHM_DICDI (Methylglutaconyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum GN=auh PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.4e-66
Identity = 135/257 (52.53%), Postives = 173/257 (67.32%), Query Frame = 1

Query: 58  LHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVMMICSSVPKVFC 117
           L RL   + GI  +   R  VKNA+GK+++   R     +   P   V+++ S V  VFC
Sbjct: 47  LERLEGENKGISVISFNRGHVKNALGKNLMNQFRSHLNELRFCPDTRVVIVRSLVDGVFC 106

Query: 118 AGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSGLEMALACDLRI 177
           +GADLKER  M+  E   +V SLR++FT LE L +PTI+ IEG A G G EM LACD R+
Sbjct: 107 SGADLKERALMSQVEASQFVHSLRSSFTELETLQMPTIAAIEGVAVGGGTEMVLACDFRV 166

Query: 178 CGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCV 237
             + +K+ LPETGLAIIPGAGGT RLPRL+G   AKELIFTG  +  + AL IGLV Y  
Sbjct: 167 ASKSSKMGLPETGLAIIPGAGGTQRLPRLIGIPRAKELIFTGAILDSKRALEIGLVQYET 226

Query: 238 PAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVEDECYKELLDTKDRL 297
             GEA+ KA+EIA++I  KGP+AI+MAK+AID G+  D  S M +E   Y +++ TKDR+
Sbjct: 227 EKGEAFDKAIEIAKQIIPKGPIAIRMAKQAIDRGMNVDQASGMIIEQASYAQVIPTKDRI 286

Query: 298 EGLTAFAEKRKPRYRGE 315
           EGLTAF EKRKP Y+GE
Sbjct: 287 EGLTAFKEKRKPIYKGE 303

BLAST of CmaCh16G011140 vs. Swiss-Prot
Match: AUHM_HUMAN (Methylglutaconyl-CoA hydratase, mitochondrial OS=Homo sapiens GN=AUH PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.2e-64
Identity = 122/265 (46.04%), Postives = 185/265 (69.81%), Query Frame = 1

Query: 54  ESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVMMICSSVP 113
           + +R+  L + + GIV + + R   KN++ K++++ L  A +++ SD     ++I S VP
Sbjct: 75  DELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVP 134

Query: 114 KVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSGLEMALAC 173
            +FCAGADLKER KM++SEV  +V+ +RA    +  L +PTI+ I+G A G GLE+ALAC
Sbjct: 135 GIFCAGADLKERAKMSSSEVGPFVSKIRAVINDIANLPVPTIAAIDGLALGGGLELALAC 194

Query: 174 DLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLV 233
           D+R+    AK+ L ET LAIIPG GGT RLPR +G S+AKELIF+ R + G++A ++GL+
Sbjct: 195 DIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLI 254

Query: 234 NYCVPAGE----AYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVEDECYKE 293
           ++ +   +    AY KAL++A+E   +GP+A+++AK AI++G+E DL + + +E+ CY +
Sbjct: 255 SHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQ 314

Query: 294 LLDTKDRLEGLTAFAEKRKPRYRGE 315
            + TKDRLEGL AF EKR PRY+GE
Sbjct: 315 TIPTKDRLEGLLAFKEKRPPRYKGE 339

BLAST of CmaCh16G011140 vs. Swiss-Prot
Match: AUHM_MOUSE (Methylglutaconyl-CoA hydratase, mitochondrial OS=Mus musculus GN=Auh PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 2.9e-64
Identity = 123/265 (46.42%), Postives = 184/265 (69.43%), Query Frame = 1

Query: 54  ESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVMMICSSVP 113
           + +R+  L + + GIV + + R   KNA+ K++L+ L  A +++ SD     ++I S VP
Sbjct: 50  DELRVRHLEEENRGIVVLGINRAYGKNALSKNLLKMLSKAVDALKSDKKVRTIIIRSEVP 109

Query: 114 KVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSGLEMALAC 173
            +FCAGADLKER KM +SEV  +V+ +R+    +  L +PTI+ I+G A G GLE+ALAC
Sbjct: 110 GIFCAGADLKERAKMHSSEVGPFVSKIRSVINDIANLPVPTIAAIDGLALGGGLELALAC 169

Query: 174 DLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLV 233
           D+R+    AK+ L ET LAIIPG GGT RLPR +G S+AKELIF+ R + G++A ++GL+
Sbjct: 170 DIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGQEAKAVGLI 229

Query: 234 NYCVPAGE----AYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVEDECYKE 293
           ++ +   +    AY KAL++A+E   +GP+A+++AK AI++G+E DL + + +E+ CY +
Sbjct: 230 SHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQ 289

Query: 294 LLDTKDRLEGLTAFAEKRKPRYRGE 315
            + TKDRLEGL AF EKR PRY+GE
Sbjct: 290 TISTKDRLEGLLAFKEKRPPRYKGE 314

BLAST of CmaCh16G011140 vs. Swiss-Prot
Match: ECHD2_MOUSE (Enoyl-CoA hydratase domain-containing protein 2, mitochondrial OS=Mus musculus GN=Echdc2 PE=1 SV=2)

HSP 1 Score: 228.8 bits (582), Expect = 8.3e-59
Identity = 124/299 (41.47%), Postives = 184/299 (61.54%), Query Frame = 1

Query: 22  PQNLSIPATLPFRTFPWRYTHCRTLILESVF--PESVRLHRLSDSDSGIVEVHLARPEVK 81
           P+ L +P +       WR++  R     +    PE +++  L+  + GI E+ + RP  +
Sbjct: 6   PRALRLPCS-------WRFSGARDCASHATTRTPE-IQVQALTGPNQGITEILMNRPNAR 65

Query: 82  NAIGKDMLRGLRYAFESIDSDPSANVMMICSSVPKVFCAGADLKERKKMTASEVHSYVTS 141
           NA+G   +  L  A   +  D    V++  S+V  VFCAGADLKER++M+  EV ++V  
Sbjct: 66  NALGNVFVSELLEALAQLREDQQVRVLLFRSAVKGVFCAGADLKEREQMSDVEVGTFVQR 125

Query: 142 LRAAFTFLEGLCIPTISVIEGAAFGSGLEMALACDLRICGEDAKLSLPETGLAIIPGAGG 201
           LR   + +    +PTI+ ++G A G GLE+ALACDLRI    A + L ET   ++PGAGG
Sbjct: 126 LRGLMSEIAAFPVPTIAAMDGFALGGGLELALACDLRIAASSAVMGLIETTRGLLPGAGG 185

Query: 202 TARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCVPAGE----AYAKALEIAQEINQ 261
           T RLPR +G ++AKELIFTGR+++G  A  +GLVN+ V   E    AY +AL +AQEI  
Sbjct: 186 TQRLPRCLGVALAKELIFTGRRLNGAQARELGLVNHAVAQNEEGNAAYHRALALAQEILP 245

Query: 262 KGPLAIKMAKKAIDEGLERDLESAMEVEDECYKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           + P+A+++ K AID G+E D+ S M +E  CY + + T+DRLEG+ AF EKR P++ G+
Sbjct: 246 QAPIAVRLGKVAIDRGMEVDIASGMAIEQMCYAQNIPTQDRLEGMAAFREKRAPKFVGK 296

BLAST of CmaCh16G011140 vs. TrEMBL
Match: A0A0A0L6S6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G017300 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 9.3e-142
Identity = 268/328 (81.71%), Postives = 284/328 (86.59%), Query Frame = 1

Query: 1   MGAFRVLCGKLINLRTQLHHSPQNLSIPA-----TLPFRT---------FPWRYTHCRTL 60
           MGAF++    LINL  +L HS QNL IPA     T+P  T          PWRYTHCRTL
Sbjct: 1   MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 60

Query: 61  ILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVM 120
           IL+S+  + VRLHRLSDSDSGIVE+HL RPE KNAI KDMLRGLR+AFES+DSDPS NVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 121 MICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSG 180
           MI SSVPKVFCAGADLKERKKM ASEVHS+VTSLR+AFTFLE L IPTISVIEGAA G G
Sbjct: 121 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 241 ALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVEDEC 300
           ALSIGLVNYCV AGEAY KALEIAQEIN+KGPLAI+MAKKAI+EGLE DLESAME+EDEC
Sbjct: 241 ALSIGLVNYCVSAGEAYTKALEIAQEINEKGPLAIRMAKKAINEGLEGDLESAMEIEDEC 300

Query: 301 YKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           Y ELLDTKDRLEGL AFAEKRKPRYRGE
Sbjct: 301 YTELLDTKDRLEGLAAFAEKRKPRYRGE 328

BLAST of CmaCh16G011140 vs. TrEMBL
Match: A0A067L5Z9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_03456 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.0e-112
Identity = 211/271 (77.86%), Postives = 236/271 (87.08%), Query Frame = 1

Query: 44  RTLILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSA 103
           RTLIL+S+  ESV+L R SDSDSGI+EV+L R   KNAIGK+MLRGL+Y FE+I  D SA
Sbjct: 55  RTLILDSLVSESVKLQRFSDSDSGIIEVNLDRAGAKNAIGKEMLRGLQYTFETISKDDSA 114

Query: 104 NVMMICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAF 163
           +V+MICSSVPKVFCAGADLKERK MT SEV  +V  LR+ F F+E LCIPTI+VIEGAA 
Sbjct: 115 SVVMICSSVPKVFCAGADLKERKTMTPSEVQDFVNRLRSTFLFIEELCIPTIAVIEGAAL 174

Query: 164 GSGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVS 223
           G GLEMALACDLRICGEDA L LPETGLAIIPGAGGT RLPRLVGKS+AKELIFTGRKV 
Sbjct: 175 GGGLEMALACDLRICGEDAVLGLPETGLAIIPGAGGTQRLPRLVGKSVAKELIFTGRKVG 234

Query: 224 GRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVE 283
           GR+A SIGLVNY VPAGEA  KALEIA+E+NQKGPLAIKMAKKAI+EGLE DL SA+E+E
Sbjct: 235 GREASSIGLVNYSVPAGEARTKALEIAREVNQKGPLAIKMAKKAINEGLEADLASALELE 294

Query: 284 DECYKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           +ECY++LL+TKDRLEGL AFAEKRKPRY GE
Sbjct: 295 EECYEQLLNTKDRLEGLAAFAEKRKPRYTGE 325

BLAST of CmaCh16G011140 vs. TrEMBL
Match: B9T286_RICCO (Methylglutaconyl-CoA hydratase, mitochondrial, putative OS=Ricinus communis GN=RCOM_0191380 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.0e-111
Identity = 207/271 (76.38%), Postives = 241/271 (88.93%), Query Frame = 1

Query: 44  RTLILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSA 103
           RTLILESV  ESV+L RL DSDSGI+EV+L RP  KNAIGK+MLRGLR  FE+I++D SA
Sbjct: 55  RTLILESVSSESVKLKRLPDSDSGIMEVNLDRPGAKNAIGKEMLRGLRNTFETINNDASA 114

Query: 104 NVMMICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAF 163
           NV+MICSSVPKVFCAGADLKERK MT SEV  +V SLR+ F+ +E LCIPTI+VIEGAA 
Sbjct: 115 NVVMICSSVPKVFCAGADLKERKIMTPSEVQFFVNSLRSTFSLVEDLCIPTIAVIEGAAL 174

Query: 164 GSGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVS 223
           G GLEMAL+CDLRICGEDA L LPETGLAIIPGAGGT RLPRLVGKS+AKELIFTGRK+ 
Sbjct: 175 GGGLEMALSCDLRICGEDAVLGLPETGLAIIPGAGGTQRLPRLVGKSVAKELIFTGRKIG 234

Query: 224 GRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVE 283
           GR+A+S+GLVNY VPAGEA  KALE+A+EINQKGP+AI+MAKKAI+EGL+ D+ SA+E+E
Sbjct: 235 GREAMSMGLVNYSVPAGEARLKALEVAREINQKGPIAIRMAKKAINEGLKVDMASALELE 294

Query: 284 DECYKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           +ECY++L+DTKDRLEGL +FAE+RKPRYRGE
Sbjct: 295 EECYEQLMDTKDRLEGLASFAERRKPRYRGE 325

BLAST of CmaCh16G011140 vs. TrEMBL
Match: A0A061G4B1_THECC (ATP-dependent caseinolytic protease/crotonase family protein OS=Theobroma cacao GN=TCM_015894 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.2e-111
Identity = 206/284 (72.54%), Postives = 243/284 (85.56%), Query Frame = 1

Query: 31  LPFRTFPWRYTHCRTLILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGL 90
           +P   +  ++   RTLILE    ESV+L RL  SDSGIVEV+L RP  +NAIG DMLRGL
Sbjct: 567 IPISNYKLQFQTLRTLILEPSLSESVKLRRLPGSDSGIVEVNLERPGARNAIGNDMLRGL 626

Query: 91  RYAFESIDSDPSANVMMICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGL 150
           R+AFE+ID D SA+V++I SSVP VFCAGADLKERKKMTASEVHSYV  LR+ F+ +E L
Sbjct: 627 RHAFEAIDGDSSAHVVLISSSVPNVFCAGADLKERKKMTASEVHSYVNYLRSTFSLIEEL 686

Query: 151 CIPTISVIEGAAFGSGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKS 210
            IPTI+VIEGAA G GLEMAL+CDLRICGE+A L LPETGLAIIPGAGGT RLPRLVG+S
Sbjct: 687 QIPTIAVIEGAALGGGLEMALSCDLRICGENAMLGLPETGLAIIPGAGGTQRLPRLVGQS 746

Query: 211 IAKELIFTGRKVSGRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDE 270
           IAKELIFT R++ GRDA+S+GLVNYCVPAGEA+ K LEIA+EINQKGP+AI+MAK+AI+E
Sbjct: 747 IAKELIFTARRIGGRDAMSMGLVNYCVPAGEAHPKGLEIAREINQKGPIAIRMAKRAINE 806

Query: 271 GLERDLESAMEVEDECYKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           GLERD+ SA+++E+ECY++LL+TKDRLEGL AFAEKRKPRY GE
Sbjct: 807 GLERDMTSALDLEEECYEQLLNTKDRLEGLAAFAEKRKPRYNGE 850

BLAST of CmaCh16G011140 vs. TrEMBL
Match: F6HCX6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0234g00080 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 8.5e-111
Identity = 211/296 (71.28%), Postives = 243/296 (82.09%), Query Frame = 1

Query: 19  HHSPQNLSIPATLPFRTFPWRYTHCRTLILESVFPESVRLHRLSDSDSGIVEVHLARPEV 78
           HH  +N S         F + Y  CRTLIL+S F ESVR+ RLS  DSGIVEVHL RPE 
Sbjct: 10  HHCRRNFS---------FNYHYQSCRTLILQSAF-ESVRVQRLSHDDSGIVEVHLDRPEA 69

Query: 79  KNAIGKDMLRGLRYAFESIDSDPSANVMMICSSVPKVFCAGADLKERKKMTASEVHSYVT 138
           KNAIGK+ML GL+  FE+I+ D SANV+M+ SSVP+VFCAGADLKERK M  SE   +V 
Sbjct: 70  KNAIGKEMLGGLQNIFEAINRDASANVVMLSSSVPRVFCAGADLKERKTMNPSETRFFVN 129

Query: 139 SLRAAFTFLEGLCIPTISVIEGAAFGSGLEMALACDLRICGEDAKLSLPETGLAIIPGAG 198
           SLR+ F+ LE L +PTI+VIEGAA G GLEMAL+CDLRICGEDA L LPETGLAIIPGAG
Sbjct: 130 SLRSTFSLLEALHVPTIAVIEGAALGGGLEMALSCDLRICGEDAVLGLPETGLAIIPGAG 189

Query: 199 GTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGP 258
           GT RL RLVGKSIAKELIFTGRKV GRDA+S+GLVNYCVPAGEA+ KALEIAQ INQKGP
Sbjct: 190 GTQRLSRLVGKSIAKELIFTGRKVGGRDAMSVGLVNYCVPAGEAHLKALEIAQHINQKGP 249

Query: 259 LAIKMAKKAIDEGLERDLESAMEVEDECYKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           LA++MAK+AI+EGLE D+ESA+ +E+ECY++LL+TKDRLEGL AF+EKRKPRY GE
Sbjct: 250 LALQMAKRAINEGLELDMESALALEEECYEQLLNTKDRLEGLAAFSEKRKPRYNGE 295

BLAST of CmaCh16G011140 vs. TAIR10
Match: AT4G16800.1 (AT4G16800.1 ATP-dependent caseinolytic (Clp) protease/crotonase family protein)

HSP 1 Score: 366.3 bits (939), Expect = 1.9e-101
Identity = 187/271 (69.00%), Postives = 224/271 (82.66%), Query Frame = 1

Query: 43  CRTLILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPS 102
           CRTLI+E+  PE V+L+RLS SDSGI+EV+L RP  KNAI K+ML+ L+ AFESI  D S
Sbjct: 30  CRTLIIETSPPEFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNS 89

Query: 103 ANVMMICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAA 162
           A V+MI S VP VFCAGADLKER+ M+ SEVH+YV SLR  F+F+E L IPTI+ IEGAA
Sbjct: 90  ARVVMIRSLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAA 149

Query: 163 FGSGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKV 222
            G GLEMALACDLRICGE+A   LPETGLAIIPGAGGT RL RLVG+S++KELIFTGRK+
Sbjct: 150 LGGGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKI 209

Query: 223 SGRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEV 282
              +A + GLVN CV AGEA+ KA+E+AQ+IN+KGPLAIKMAKKAIDEG+E ++ S +EV
Sbjct: 210 DAIEAANKGLVNICVTAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEV 269

Query: 283 EDECYKELLDTKDRLEGLTAFAEKRKPRYRG 314
           E+ CY++LL+T+DRLEGL AFAEKRKP Y G
Sbjct: 270 EEMCYQKLLNTQDRLEGLAAFAEKRKPLYTG 300

BLAST of CmaCh16G011140 vs. TAIR10
Match: AT5G43280.1 (AT5G43280.1 delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1)

HSP 1 Score: 122.1 bits (305), Expect = 6.1e-28
Identity = 81/262 (30.92%), Postives = 128/262 (48.85%), Query Frame = 1

Query: 63  DSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVMMICSSVPKVFCAGADL 122
           ++DS +  + + RP   NA+  D       A  S+D +P  +V+++ S   K FC+G DL
Sbjct: 15  NTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL-SGAGKHFCSGIDL 74

Query: 123 KERKKMT------------ASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSGLEMA 182
                ++            + ++   + S++AA T +E    P I+ I GA  G G+++ 
Sbjct: 75  NSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIHGACIGGGVDLI 134

Query: 183 LACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSI 242
            ACD+R C EDA  S+ E  LAI+   G   RLP +VG + A EL  T R+ SG +A  +
Sbjct: 135 TACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTARRFSGSEAKDL 194

Query: 243 GLVNYCVPA-GEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVEDECYKE 302
           GLV+    +  E       IA+ I  K PLA+   K  +    E  +E  ++        
Sbjct: 195 GLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQGLDYVATWNSA 254

Query: 303 LLDTKDRLEGLTAFAEKRKPRY 312
           +L + D  E ++A   KRKPR+
Sbjct: 255 MLISDDLNEAVSAQMMKRKPRF 275

BLAST of CmaCh16G011140 vs. TAIR10
Match: AT4G16210.1 (AT4G16210.1 enoyl-CoA hydratase/isomerase A)

HSP 1 Score: 107.1 bits (266), Expect = 2.0e-23
Identity = 72/231 (31.17%), Postives = 114/231 (49.35%), Query Frame = 1

Query: 55  SVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVMMICSSVPK 114
           S  L ++     GI  + + RP+  N++ + M+  L  AF+ +DSD S  V++   S  +
Sbjct: 6   SENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGS-GR 65

Query: 115 VFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSGLEMALACD 174
            FC+G DL   + +   +V    T        +E L  P I  I G A  +G E+ALACD
Sbjct: 66  SFCSGVDLTAAESVFKGDVKDPETD---PVVQMERLRKPIIGAINGFAITAGFELALACD 125

Query: 175 LRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVN 234
           + +    AK         I P  G + +L R++G + A+E+  T   ++   A  +G VN
Sbjct: 126 ILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFVN 185

Query: 235 YCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVEDE 286
           + V  GEA  KA EIA+ I +     +   K  I++GL+ DL  A+ +E E
Sbjct: 186 HVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKE 232

BLAST of CmaCh16G011140 vs. TAIR10
Match: AT3G06860.1 (AT3G06860.1 multifunctional protein 2)

HSP 1 Score: 101.7 bits (252), Expect = 8.6e-22
Identity = 66/190 (34.74%), Postives = 97/190 (51.05%), Query Frame = 1

Query: 67  GIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVMMICSSVPKVFCAGADLK--- 126
           G+  + L  P V N++  D+L  L+  +E   S      ++I  +  + F  G D+    
Sbjct: 17  GVAVITLINPPV-NSLSFDVLYNLKSNYEEALSRNDVKAIVITGAKGR-FSGGFDISGFG 76

Query: 127 ERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSGLEMALACDLRICGEDAK 186
           E +K    E  +   S+      LE    P+++ I+G A G GLE+A+AC  RI    A+
Sbjct: 77  EMQKGNVKEPKAGYISIDIITDLLEAARKPSVAAIDGLALGGGLELAMACHARISAPAAQ 136

Query: 187 LSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCVPAGEAY 246
           L LPE  L +IPG GGT RLPRLVG + A E+I T + V   +  S+GL++  VP  E  
Sbjct: 137 LGLPELQLGVIPGFGGTQRLPRLVGLTKALEMILTSKPVKAEEGHSLGLIDAVVPPAELV 196

Query: 247 AKALEIAQEI 254
             A   A +I
Sbjct: 197 TTARRWALDI 204

BLAST of CmaCh16G011140 vs. TAIR10
Match: AT4G29010.1 (AT4G29010.1 Enoyl-CoA hydratase/isomerase family)

HSP 1 Score: 95.1 bits (235), Expect = 8.0e-20
Identity = 54/191 (28.27%), Postives = 100/191 (52.36%), Query Frame = 1

Query: 65  DSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVMMICSSVPKVFCAGADLKE 124
           + G+  + ++ P V N++   ++ GL+  F   +       +++  +  + F  G D+  
Sbjct: 13  NDGVAVITISNPPV-NSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGR-FSGGFDINV 72

Query: 125 RKKMTASEVHSYV--TSLRAAFTFLEGLCIPTISVIEGAAFGSGLEMALACDLRICGEDA 184
            +++  +   S +   S+      +E    P ++ +EG A G GLE+A+AC  R+    A
Sbjct: 73  FQQVHKTGDLSLMPEVSVELVCNLMEDSRKPVVAAVEGLALGGGLELAMACHARVAAPKA 132

Query: 185 KLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRDALSIGLVNYCVPAGEA 244
           +L LPE  L +IPG GGT RLPRLVG + A ++I   + +S  +   +GL++  VP G+ 
Sbjct: 133 QLGLPELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISSEEGHKLGLIDALVPPGDV 192

Query: 245 YAKALEIAQEI 254
            + + + A +I
Sbjct: 193 LSTSRKWALDI 201

BLAST of CmaCh16G011140 vs. NCBI nr
Match: gi|659095860|ref|XP_008448801.1| (PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 537.0 bits (1382), Expect = 2.3e-149
Identity = 279/328 (85.06%), Postives = 290/328 (88.41%), Query Frame = 1

Query: 1   MGAFRVLCGKLINLRTQLHHSPQNLSIPATLPFRTFP--------------WRYTHCRTL 60
           MGAF++ C  LINLR QL+ SPQNL IPA    +TFP              WRYTHCR L
Sbjct: 1   MGAFKIRCRNLINLRIQLNRSPQNLPIPANNLRQTFPSSTPSDAFNSFSHPWRYTHCRNL 60

Query: 61  ILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVM 120
           IL+S+  + VRLHRLSDSDSGIVEVHL RPE KNAI KDMLRGLR+AFES+DSDPS NVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEVHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 121 MICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSG 180
           MICSSVPKVFCAGADLKERKKMTASEVHS+VTSLR+AFTFLE L IPTISVIEGAA G G
Sbjct: 121 MICSSVPKVFCAGADLKERKKMTASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 241 ALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVEDEC 300
           ALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLE DLESAMEVEDEC
Sbjct: 241 ALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLEGDLESAMEVEDEC 300

Query: 301 YKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           YKELLDTKDRLEGL AFAEKRKPRYRGE
Sbjct: 301 YKELLDTKDRLEGLAAFAEKRKPRYRGE 328

BLAST of CmaCh16G011140 vs. NCBI nr
Match: gi|659095858|ref|XP_008448800.1| (PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 529.6 bits (1363), Expect = 3.6e-147
Identity = 279/336 (83.04%), Postives = 290/336 (86.31%), Query Frame = 1

Query: 1   MGAFRVLCGKLINLRTQLHHSPQNLSIPATLPFRTFP--------------WRYTHCRTL 60
           MGAF++ C  LINLR QL+ SPQNL IPA    +TFP              WRYTHCR L
Sbjct: 1   MGAFKIRCRNLINLRIQLNRSPQNLPIPANNLRQTFPSSTPSDAFNSFSHPWRYTHCRNL 60

Query: 61  ILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVM 120
           IL+S+  + VRLHRLSDSDSGIVEVHL RPE KNAI KDMLRGLR+AFES+DSDPS NVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEVHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 121 MICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSG 180
           MICSSVPKVFCAGADLKERKKMTASEVHS+VTSLR+AFTFLE L IPTISVIEGAA G G
Sbjct: 121 MICSSVPKVFCAGADLKERKKMTASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 181 LEMALACDLRIC--------GEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT 240
           LEMALACDLRIC        GEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT
Sbjct: 181 LEMALACDLRICAHMVGHDLGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT 240

Query: 241 GRKVSGRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLES 300
           GRKVSGRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLE DLES
Sbjct: 241 GRKVSGRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLEGDLES 300

Query: 301 AMEVEDECYKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           AMEVEDECYKELLDTKDRLEGL AFAEKRKPRYRGE
Sbjct: 301 AMEVEDECYKELLDTKDRLEGLAAFAEKRKPRYRGE 336

BLAST of CmaCh16G011140 vs. NCBI nr
Match: gi|700200703|gb|KGN55836.1| (hypothetical protein Csa_3G017300 [Cucumis sativus])

HSP 1 Score: 511.1 bits (1315), Expect = 1.3e-141
Identity = 268/328 (81.71%), Postives = 284/328 (86.59%), Query Frame = 1

Query: 1   MGAFRVLCGKLINLRTQLHHSPQNLSIPA-----TLPFRT---------FPWRYTHCRTL 60
           MGAF++    LINL  +L HS QNL IPA     T+P  T          PWRYTHCRTL
Sbjct: 1   MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 60

Query: 61  ILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVM 120
           IL+S+  + VRLHRLSDSDSGIVE+HL RPE KNAI KDMLRGLR+AFES+DSDPS NVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 121 MICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSG 180
           MI SSVPKVFCAGADLKERKKM ASEVHS+VTSLR+AFTFLE L IPTISVIEGAA G G
Sbjct: 121 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240
           LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD
Sbjct: 181 LEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVSGRD 240

Query: 241 ALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVEDEC 300
           ALSIGLVNYCV AGEAY KALEIAQEIN+KGPLAI+MAKKAI+EGLE DLESAME+EDEC
Sbjct: 241 ALSIGLVNYCVSAGEAYTKALEIAQEINEKGPLAIRMAKKAINEGLEGDLESAMEIEDEC 300

Query: 301 YKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           Y ELLDTKDRLEGL AFAEKRKPRYRGE
Sbjct: 301 YTELLDTKDRLEGLAAFAEKRKPRYRGE 328

BLAST of CmaCh16G011140 vs. NCBI nr
Match: gi|778675284|ref|XP_011650382.1| (PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial [Cucumis sativus])

HSP 1 Score: 503.8 bits (1296), Expect = 2.1e-139
Identity = 268/336 (79.76%), Postives = 284/336 (84.52%), Query Frame = 1

Query: 1   MGAFRVLCGKLINLRTQLHHSPQNLSIPA-----TLPFRT---------FPWRYTHCRTL 60
           MGAF++    LINL  +L HS QNL IPA     T+P  T          PWRYTHCRTL
Sbjct: 1   MGAFKIRSRNLINLPIELLHSSQNLPIPAKNLRQTVPSSTPSDAFNLFSHPWRYTHCRTL 60

Query: 61  ILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSANVM 120
           IL+S+  + VRLHRLSDSDSGIVE+HL RPE KNAI KDMLRGLR+AFES+DSDPS NVM
Sbjct: 61  ILDSLSTKPVRLHRLSDSDSGIVEIHLDRPEAKNAISKDMLRGLRHAFESVDSDPSVNVM 120

Query: 121 MICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAFGSG 180
           MI SSVPKVFCAGADLKERKKM ASEVHS+VTSLR+AFTFLE L IPTISVIEGAA G G
Sbjct: 121 MIRSSVPKVFCAGADLKERKKMAASEVHSFVTSLRSAFTFLEALPIPTISVIEGAALGGG 180

Query: 181 LEMALACDLRIC--------GEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT 240
           LEMALACDLRIC        GEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT
Sbjct: 181 LEMALACDLRICAHMVGHDPGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFT 240

Query: 241 GRKVSGRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLES 300
           GRKVSGRDALSIGLVNYCV AGEAY KALEIAQEIN+KGPLAI+MAKKAI+EGLE DLES
Sbjct: 241 GRKVSGRDALSIGLVNYCVSAGEAYTKALEIAQEINEKGPLAIRMAKKAINEGLEGDLES 300

Query: 301 AMEVEDECYKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           AME+EDECY ELLDTKDRLEGL AFAEKRKPRYRGE
Sbjct: 301 AMEIEDECYTELLDTKDRLEGLAAFAEKRKPRYRGE 336

BLAST of CmaCh16G011140 vs. NCBI nr
Match: gi|802582648|ref|XP_012070065.1| (PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial isoform X2 [Jatropha curcas])

HSP 1 Score: 413.7 bits (1062), Expect = 2.9e-112
Identity = 211/271 (77.86%), Postives = 236/271 (87.08%), Query Frame = 1

Query: 44  RTLILESVFPESVRLHRLSDSDSGIVEVHLARPEVKNAIGKDMLRGLRYAFESIDSDPSA 103
           RTLIL+S+  ESV+L R SDSDSGI+EV+L R   KNAIGK+MLRGL+Y FE+I  D SA
Sbjct: 55  RTLILDSLVSESVKLQRFSDSDSGIIEVNLDRAGAKNAIGKEMLRGLQYTFETISKDDSA 114

Query: 104 NVMMICSSVPKVFCAGADLKERKKMTASEVHSYVTSLRAAFTFLEGLCIPTISVIEGAAF 163
           +V+MICSSVPKVFCAGADLKERK MT SEV  +V  LR+ F F+E LCIPTI+VIEGAA 
Sbjct: 115 SVVMICSSVPKVFCAGADLKERKTMTPSEVQDFVNRLRSTFLFIEELCIPTIAVIEGAAL 174

Query: 164 GSGLEMALACDLRICGEDAKLSLPETGLAIIPGAGGTARLPRLVGKSIAKELIFTGRKVS 223
           G GLEMALACDLRICGEDA L LPETGLAIIPGAGGT RLPRLVGKS+AKELIFTGRKV 
Sbjct: 175 GGGLEMALACDLRICGEDAVLGLPETGLAIIPGAGGTQRLPRLVGKSVAKELIFTGRKVG 234

Query: 224 GRDALSIGLVNYCVPAGEAYAKALEIAQEINQKGPLAIKMAKKAIDEGLERDLESAMEVE 283
           GR+A SIGLVNY VPAGEA  KALEIA+E+NQKGPLAIKMAKKAI+EGLE DL SA+E+E
Sbjct: 235 GREASSIGLVNYSVPAGEARTKALEIAREVNQKGPLAIKMAKKAINEGLEADLASALELE 294

Query: 284 DECYKELLDTKDRLEGLTAFAEKRKPRYRGE 315
           +ECY++LL+TKDRLEGL AFAEKRKPRY GE
Sbjct: 295 EECYEQLLNTKDRLEGLAAFAEKRKPRYTGE 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ECH2M_ARATH3.3e-10069.00Probable enoyl-CoA hydratase 2, mitochondrial OS=Arabidopsis thaliana GN=At4g168... [more]
AUHM_DICDI1.4e-6652.53Methylglutaconyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum GN=auh... [more]
AUHM_HUMAN2.2e-6446.04Methylglutaconyl-CoA hydratase, mitochondrial OS=Homo sapiens GN=AUH PE=1 SV=1[more]
AUHM_MOUSE2.9e-6446.42Methylglutaconyl-CoA hydratase, mitochondrial OS=Mus musculus GN=Auh PE=1 SV=1[more]
ECHD2_MOUSE8.3e-5941.47Enoyl-CoA hydratase domain-containing protein 2, mitochondrial OS=Mus musculus G... [more]
Match NameE-valueIdentityDescription
A0A0A0L6S6_CUCSA9.3e-14281.71Uncharacterized protein OS=Cucumis sativus GN=Csa_3G017300 PE=3 SV=1[more]
A0A067L5Z9_JATCU2.0e-11277.86Uncharacterized protein OS=Jatropha curcas GN=JCGZ_03456 PE=3 SV=1[more]
B9T286_RICCO1.0e-11176.38Methylglutaconyl-CoA hydratase, mitochondrial, putative OS=Ricinus communis GN=R... [more]
A0A061G4B1_THECC2.2e-11172.54ATP-dependent caseinolytic protease/crotonase family protein OS=Theobroma cacao ... [more]
F6HCX6_VITVI8.5e-11171.28Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0234g00080 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT4G16800.11.9e-10169.00 ATP-dependent caseinolytic (Clp) protease/crotonase family protein[more]
AT5G43280.16.1e-2830.92 delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1[more]
AT4G16210.12.0e-2331.17 enoyl-CoA hydratase/isomerase A[more]
AT3G06860.18.6e-2234.74 multifunctional protein 2[more]
AT4G29010.18.0e-2028.27 Enoyl-CoA hydratase/isomerase family[more]
Match NameE-valueIdentityDescription
gi|659095860|ref|XP_008448801.1|2.3e-14985.06PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial isoform X2 [Cucumis mel... [more]
gi|659095858|ref|XP_008448800.1|3.6e-14783.04PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial isoform X1 [Cucumis mel... [more]
gi|700200703|gb|KGN55836.1|1.3e-14181.71hypothetical protein Csa_3G017300 [Cucumis sativus][more]
gi|778675284|ref|XP_011650382.1|2.1e-13979.76PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial [Cucumis sativus][more]
gi|802582648|ref|XP_012070065.1|2.9e-11277.86PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial isoform X2 [Jatropha cu... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001753Enoyl-CoA_hydra/iso
IPR014748Enoyl-CoA_hydra_C
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006550 isoleucine catabolic process
biological_process GO:0006552 leucine catabolic process
biological_process GO:0006574 valine catabolic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004490 methylglutaconyl-CoA hydratase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G011140.1CmaCh16G011140.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001753Crotonase superfamilyPFAMPF00378ECH_1coord: 65..313
score: 3.6
IPR014748Crontonase, C-terminalGENE3DG3DSA:1.10.12.10coord: 256..314
score: 6.7
NoneNo IPR availableunknownCoilCoilcoord: 311..314
scor
NoneNo IPR availablePANTHERPTHR11941ENOYL-COA HYDRATASE-RELATEDcoord: 41..311
score: 9.3E
NoneNo IPR availablePANTHERPTHR11941:SF61PROTEIN ECH-5coord: 41..311
score: 9.3E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh16G011140CmaCh06G008040Cucurbita maxima (Rimu)cmacmaB356
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh16G011140Cucurbita maxima (Rimu)cmacmaB347
CmaCh16G011140Cucurbita moschata (Rifu)cmacmoB334
CmaCh16G011140Cucurbita pepo (Zucchini)cmacpeB359
CmaCh16G011140Silver-seed gourdcarcmaB1078