CmaCh16G010010 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh16G010010
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Subtilisin-like serine protease
Location	Cma_Chr16 : 7711593 .. 7712370 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTACATGACTCACATGACTGAGTTGGGAATCAAACCGACACCAATTATAGCTCCATTCTCGTCGAGAGGTCCCAACACAGTTGAGCCCTTAATACTCAAGGCTGGTTAAGAACGATGTGTTGAGTAATTTTATAGTATAAAGCTCAATGTGTCAATTGCTAACAATCACAAAATTTTAACTACTGCCTCTTTTTTTTCAAATTTCCAACCTGATATAACAGCACCAGGTGTGAATATAATAGCTGCTTTCTCTGAAGCAACATCCATAACTGGTTTACCTTGTGATAAGCGTCGGGTTCAATTCATTGAATTATCTGGTACTTCTATGTCATGCCCCCATATTTCTGGCATAGTTGGTCTTCTCAAAACCCTTTATCCAAAATGGAGTCCAACAGCTATCAAATCTACAATCATGACTACAGGTTCATTCTTCAAAATTCTTCAATCATTTTAAAATTTAATAGACTAATTAAACTAGTAATTTATGAATAGTAAGAAATGTTATTTGTGGTATTTACATTAAGAAAGATTTTGACTTAATTCTTGCCCACTAATGGTTTTGTTTTGTAGCTGTTGCGGAAGCCAATGACTTAAATCCAATAATAAGCTCAGAAAAGGAGAAAGCAAACCCATTAGCATATGGTGCAGGTCACGTCCAACCAAACAAAGCAGCAAATCCAGGCCTTGTTTATGACCTCTCCACCCAAAACTACTTGAACTTTTTATGTGCTCGTGGCTACAATAACAAAACAAATGAAACTATTCTCCAATGA

mRNA sequence

ATGGCTTACATGACTCACATGACTGAGTTGGGAATCAAACCGACACCAATTATAGCTCCATTCTCGTCGAGAGGTCCCAACACAGTTGAGCCCTTAATACTCAAGGCTGCACCAGGTGTGAATATAATAGCTGCTTTCTCTGAAGCAACATCCATAACTGGTTTACCTTGTGATAAGCGTCGGGTTCAATTCATTGAATTATCTGGTACTTCTATGTCATGCCCCCATATTTCTGGCATAGTTGGTCTTCTCAAAACCCTTTATCCAAAATGGAGTCCAACAGCTATCAAATCTACAATCATGACTACAGCTGTTGCGGAAGCCAATGACTTAAATCCAATAATAAGCTCAGAAAAGGAGAAAGCAAACCCATTAGCATATGGTGCAGGTCACGTCCAACCAAACAAAGCAGCAAATCCAGGCCTTGTTTATGACCTCTCCACCCAAAACTACTTGAACTTTTTATGTGCTCGTGGCTACAATAACAAAACAAATGAAACTATTCTCCAATGA

Coding sequence (CDS)

ATGGCTTACATGACTCACATGACTGAGTTGGGAATCAAACCGACACCAATTATAGCTCCATTCTCGTCGAGAGGTCCCAACACAGTTGAGCCCTTAATACTCAAGGCTGCACCAGGTGTGAATATAATAGCTGCTTTCTCTGAAGCAACATCCATAACTGGTTTACCTTGTGATAAGCGTCGGGTTCAATTCATTGAATTATCTGGTACTTCTATGTCATGCCCCCATATTTCTGGCATAGTTGGTCTTCTCAAAACCCTTTATCCAAAATGGAGTCCAACAGCTATCAAATCTACAATCATGACTACAGCTGTTGCGGAAGCCAATGACTTAAATCCAATAATAAGCTCAGAAAAGGAGAAAGCAAACCCATTAGCATATGGTGCAGGTCACGTCCAACCAAACAAAGCAGCAAATCCAGGCCTTGTTTATGACCTCTCCACCCAAAACTACTTGAACTTTTTATGTGCTCGTGGCTACAATAACAAAACAAATGAAACTATTCTCCAATGA

Protein sequence

MAYMTHMTELGIKPTPIIAPFSSRGPNTVEPLILKAAPGVNIIAAFSEATSITGLPCDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIISSEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYNNKTNETILQ

Homology

[Close]

BLAST of CmaCh16G010010 vs. Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.0e-50
Identity = 101/156 (64.74%), Postives = 117/156 (75.00%), Query Frame = 1


Query: 10  LGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLPCDKRRVQFIE 69
           L  KP P +A FSSRGPNT+ P ILK    APGVNIIAAF+EAT  T L  D RR  F  
Sbjct: 503 LNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNT 562

Query: 70  LSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIISSEKEKANPLA 129
            SGTSMSCPHISG+VGLLKTL+P WSP AI+S IMTT+    N   P++    +KANP +
Sbjct: 563 ESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFS 622

Query: 130 YGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYNN 163
           YG+GHVQPNKAA+PGLVYDL+T +YL+FLCA GYNN
Sbjct: 623 YGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNN 658

[Close]

BLAST of CmaCh16G010010 vs. Swiss-Prot
Match: AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 4.3e-46
Identity = 93/157 (59.24%), Postives = 114/157 (72.61%), Query Frame = 1


Query: 8   TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLPCDKRRVQF 67
           T+LG+KP P++A FSS+GP+ V P ILK    APGV++IAA++ A S T    D RR+ F
Sbjct: 495 TDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLF 554

Query: 68  IELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIISSEKEKANP 127
             +SGTSMSCPHISGI GLLKT YP WSP AI+S IMTTA    +   PI ++   KA P
Sbjct: 555 NAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATP 614

Query: 128 LAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
            ++GAGHVQPN A NPGLVYDL  ++YLNFLC+ GYN
Sbjct: 615 FSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYN 651

[Close]

BLAST of CmaCh16G010010 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.0e-43
Identity = 88/157 (56.05%), Postives = 113/157 (71.97%), Query Frame = 1


Query: 8   TELGIKPTPIIAPFSSRGPNTVEPLILKA---APGVNIIAAFSEATSITGLPCDKRRVQF 67
           T L +KP+P++A FSSRGPNTV P ILK     PGVNI+A +S+A   TGL  D RR QF
Sbjct: 470 TVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQF 529

Query: 68  IELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPI-ISSEKEKAN 127
             +SGTSMSCPHISG+ GLLK  +P+WSP+AIKS +MTTA    N   P+  +++   +N
Sbjct: 530 NIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN 589

Query: 128 PLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGY 161
           P A+G+GHV P KA +PGLVYD+ST+ Y+ FLC+  Y
Sbjct: 590 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDY 626

[Close]

BLAST of CmaCh16G010010 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.4e-43
Identity = 85/159 (53.46%), Postives = 114/159 (71.70%), Query Frame = 1


Query: 8   TELGIKPTPIIAPFSSRGPNTVEPLILKA---APGVNIIAAFSEATSITGLPCDKRRVQF 67
           T +G+KP+P++A FSSRGPN++ P ILK    APGVNI+AA++ A   TGL  D RRV+F
Sbjct: 476 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 535

Query: 68  IELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIISSEKEK-AN 127
             +SGTSMSCPH+SG+  LLK+++P+WSP AI+S +MTTA     D  P++     K + 
Sbjct: 536 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPST 595

Query: 128 PLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYNN 163
           P  +GAGHV P  A NPGL+YDL+T++YL FLCA  Y +
Sbjct: 596 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTS 634

[Close]

BLAST of CmaCh16G010010 vs. Swiss-Prot
Match: SBT1_SOYBN (Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 172.9 bits (437), Expect = 2.9e-42
Identity = 93/161 (57.76%), Postives = 111/161 (68.94%), Query Frame = 1


Query: 8   TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLPCDKRR-VQ 67
           T  G KP P++A FSSRGPN ++P ILK    APGVNI+AA+SE  S + L  D RR  +
Sbjct: 509 TLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFK 568

Query: 68  FIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIISS-EKEKA 127
           F  L GTSMSCPH SGI GLLKT +P WSP AIKS IMTTA    N   PI  + +K  A
Sbjct: 569 FNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLA 628

Query: 128 NPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYNNK 164
           +  AYG+GHV+P+ A  PGLVYDLS  +YLNFLCA GY+ +
Sbjct: 629 DAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQ 669

[Close]

BLAST of CmaCh16G010010 vs. TrEMBL
Match: A0A0A0L2R9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 3.5e-58
Identity = 117/165 (70.91%), Postives = 135/165 (81.82%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           MAYMTH+ TE GIKP P++A FSSRGPNTV+P ILK    APG NI+AA+S   S TG  
Sbjct: 471 MAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGTD 530

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            DKRRV F   SGTSMSCPH+SGIVGLLKTLYPKWSP AI+S IMTTA  +ANDL PI+S
Sbjct: 531 FDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILS 590

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
           + +EKAN  AYGAGHV PN+AA+PGLVYDLST++YLN+LCA+GYN
Sbjct: 591 TNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYN 635

[Close]

BLAST of CmaCh16G010010 vs. TrEMBL
Match: A0A0A0L613_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045110 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 5.0e-57
Identity = 116/165 (70.30%), Postives = 134/165 (81.21%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           MAYMTH+ TELGIKP P++A FSSRGPNTVE  ILK    APGVNI+AA+SE  S +G  
Sbjct: 347 MAYMTHVRTELGIKPAPVMASFSSRGPNTVEETILKPDITAPGVNILAAYSEDASPSGSF 406

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            DKRR+ F  +SGTSMSCPHISGIVGLLKTLYP WSP AIKS IMTTA + AND++PI +
Sbjct: 407 FDKRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAESRANDMHPIQN 466

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
               KAN  AYGAGHVQPN+A NPGLVYDL+T++Y+NFLCA+GYN
Sbjct: 467 GGNLKANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLCAQGYN 511

[Close]

BLAST of CmaCh16G010010 vs. TrEMBL
Match: W9QR32_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_023738 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 3.7e-52
Identity = 109/174 (62.64%), Postives = 131/174 (75.29%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           MAYMT + TE+GIKP P +A FSSRGPNT+E  +LK    APGVNIIAA+++A   T   
Sbjct: 445 MAYMTRVKTEVGIKPAPFMASFSSRGPNTIEEALLKPDITAPGVNIIAAYTQAAGPTDQD 504

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            DKRRV F   SGTSMSCPH+SGIVGLLKTL+P WSP AIKS IMT+A    ++  P++ 
Sbjct: 505 FDKRRVPFNTESGTSMSCPHVSGIVGLLKTLHPDWSPAAIKSAIMTSARTRDDNKEPLLD 564

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYNNKTNETILQ 171
           S  +KA P AYGAGH+QPN A +PGLVYDL+T +YLNFLCARGY    NET+L+
Sbjct: 565 SSMKKATPFAYGAGHIQPNHAMDPGLVYDLTTDDYLNFLCARGY----NETLLK 614

[Close]

BLAST of CmaCh16G010010 vs. TrEMBL
Match: W9R162_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_023751 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 7.0e-51
Identity = 102/165 (61.82%), Postives = 128/165 (77.58%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           MAYMT + TE+G++P P++A FSSRGPNT+EP ILK    APGV+IIAA+S+A   T L 
Sbjct: 461 MAYMTGVKTEVGVRPAPLMASFSSRGPNTIEPAILKPDITAPGVDIIAAYSKAAPPTYLT 520

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            D RRV FI  SGTS SCPH+SGI GLLKTL+P WSP AIKS IMTTA A  N++ P+++
Sbjct: 521 SDTRRVSFIAQSGTSTSCPHVSGIAGLLKTLHPDWSPAAIKSAIMTTARARDNNMEPMLN 580

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
           S  ++  P AYGAGH++PN+A +PGLVYDL+  +YL+FLCA GYN
Sbjct: 581 SSMKRETPFAYGAGHIRPNRAMDPGLVYDLTIDDYLDFLCAHGYN 625

[Close]

BLAST of CmaCh16G010010 vs. TrEMBL
Match: A0A151SLF4_CAJCA (Subtilisin-like protease OS=Cajanus cajan GN=KK1_001840 PE=4 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 2.0e-50
Identity = 101/166 (60.84%), Postives = 122/166 (73.49%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           +AY++ + TELG+KP P +A FSS+GPN  EP ILK    APGVNIIAA+SEA S+T L 
Sbjct: 482 VAYISRVKTELGVKPAPYVASFSSKGPNHFEPAILKPDVTAPGVNIIAAYSEAVSLTELD 541

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            DKRR  F  LSGTSMSCPH++G+ GLLK L+P WSP AIKS I+T+A    N   PI+ 
Sbjct: 542 SDKRRTPFFTLSGTSMSCPHVAGLAGLLKALHPDWSPAAIKSAIITSATTLDNSRKPILD 601

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYNN 163
               KA P  YGAGH+QPN+A NPGLVYDL+  +YLNFLC RGYN+
Sbjct: 602 ESLNKATPFDYGAGHIQPNRAMNPGLVYDLNITDYLNFLCGRGYNS 647

[Close]

BLAST of CmaCh16G010010 vs. TAIR10
Match: AT5G59810.1 (AT5G59810.1 Subtilase family protein)

HSP 1 Score: 201.1 bits (510), Expect = 5.6e-52
Identity = 101/156 (64.74%), Postives = 117/156 (75.00%), Query Frame = 1


Query: 10  LGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLPCDKRRVQFIE 69
           L  KP P +A FSSRGPNT+ P ILK    APGVNIIAAF+EAT  T L  D RR  F  
Sbjct: 503 LNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNT 562

Query: 70  LSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIISSEKEKANPLA 129
            SGTSMSCPHISG+VGLLKTL+P WSP AI+S IMTT+    N   P++    +KANP +
Sbjct: 563 ESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFS 622

Query: 130 YGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYNN 163
           YG+GHVQPNKAA+PGLVYDL+T +YL+FLCA GYNN
Sbjct: 623 YGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNN 658

[Close]

BLAST of CmaCh16G010010 vs. TAIR10
Match: AT2G04160.1 (AT2G04160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 185.7 bits (470), Expect = 2.5e-47
Identity = 93/157 (59.24%), Postives = 114/157 (72.61%), Query Frame = 1


Query: 8   TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLPCDKRRVQF 67
           T+LG+KP P++A FSS+GP+ V P ILK    APGV++IAA++ A S T    D RR+ F
Sbjct: 495 TDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLF 554

Query: 68  IELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIISSEKEKANP 127
             +SGTSMSCPHISGI GLLKT YP WSP AI+S IMTTA    +   PI ++   KA P
Sbjct: 555 NAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATP 614

Query: 128 LAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
            ++GAGHVQPN A NPGLVYDL  ++YLNFLC+ GYN
Sbjct: 615 FSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYN 651

[Close]

BLAST of CmaCh16G010010 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 176.8 bits (447), Expect = 1.1e-44
Identity = 88/157 (56.05%), Postives = 113/157 (71.97%), Query Frame = 1


Query: 8   TELGIKPTPIIAPFSSRGPNTVEPLILKA---APGVNIIAAFSEATSITGLPCDKRRVQF 67
           T L +KP+P++A FSSRGPNTV P ILK     PGVNI+A +S+A   TGL  D RR QF
Sbjct: 470 TVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQF 529

Query: 68  IELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPI-ISSEKEKAN 127
             +SGTSMSCPHISG+ GLLK  +P+WSP+AIKS +MTTA    N   P+  +++   +N
Sbjct: 530 NIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN 589

Query: 128 PLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGY 161
           P A+G+GHV P KA +PGLVYD+ST+ Y+ FLC+  Y
Sbjct: 590 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDY 626

[Close]

BLAST of CmaCh16G010010 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 176.0 bits (445), Expect = 1.9e-44
Identity = 85/159 (53.46%), Postives = 114/159 (71.70%), Query Frame = 1


Query: 8   TELGIKPTPIIAPFSSRGPNTVEPLILKA---APGVNIIAAFSEATSITGLPCDKRRVQF 67
           T +G+KP+P++A FSSRGPN++ P ILK    APGVNI+AA++ A   TGL  D RRV+F
Sbjct: 476 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 535

Query: 68  IELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIISSEKEK-AN 127
             +SGTSMSCPH+SG+  LLK+++P+WSP AI+S +MTTA     D  P++     K + 
Sbjct: 536 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPST 595

Query: 128 PLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYNN 163
           P  +GAGHV P  A NPGL+YDL+T++YL FLCA  Y +
Sbjct: 596 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTS 634

[Close]

BLAST of CmaCh16G010010 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 164.1 bits (414), Expect = 7.6e-41
Identity = 82/165 (49.70%), Postives = 112/165 (67.88%), Query Frame = 1


Query: 8   TELGIKPTPIIAPFSSRGPNTVEPLILKA---APGVNIIAAFSEATSITGLPCDKRRVQF 67
           T +GIKP P+IA FS RGPN + P ILK    APGVNI+AA+++A   TGLP D R+ +F
Sbjct: 472 TIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEF 531

Query: 68  IELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTA-VAEANDLNPIISSEKEKAN 127
             LSGTSM+CPH+SG   LLK+ +P WSP  I+S +MTT  + + ++ + I  S  + A 
Sbjct: 532 NILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSAT 591

Query: 128 PLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYNNKTNETI 169
           P  YG+GH+   +A NPGLVYD++  +Y+ FLC+ GY  KT + I
Sbjct: 592 PYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVI 636

[Close]

BLAST of CmaCh16G010010 vs. NCBI nr
Match: gi|778675733|ref|XP_011650462.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 232.6 bits (592), Expect = 5.0e-58
Identity = 117/165 (70.91%), Postives = 135/165 (81.82%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           MAYMTH+ TE GIKP P++A FSSRGPNTV+P ILK    APG NI+AA+S   S TG  
Sbjct: 430 MAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGTD 489

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            DKRRV F   SGTSMSCPH+SGIVGLLKTLYPKWSP AI+S IMTTA  +ANDL PI+S
Sbjct: 490 FDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILS 549

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
           + +EKAN  AYGAGHV PN+AA+PGLVYDLST++YLN+LCA+GYN
Sbjct: 550 TNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYN 594

[Close]

BLAST of CmaCh16G010010 vs. NCBI nr
Match: gi|700200865|gb|KGN55998.1| (hypothetical protein Csa_3G045120 [Cucumis sativus])

HSP 1 Score: 232.6 bits (592), Expect = 5.0e-58
Identity = 117/165 (70.91%), Postives = 135/165 (81.82%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           MAYMTH+ TE GIKP P++A FSSRGPNTV+P ILK    APG NI+AA+S   S TG  
Sbjct: 471 MAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGTD 530

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            DKRRV F   SGTSMSCPH+SGIVGLLKTLYPKWSP AI+S IMTTA  +ANDL PI+S
Sbjct: 531 FDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILS 590

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
           + +EKAN  AYGAGHV PN+AA+PGLVYDLST++YLN+LCA+GYN
Sbjct: 591 TNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYN 635

[Close]

BLAST of CmaCh16G010010 vs. NCBI nr
Match: gi|659096606|ref|XP_008449186.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103491136 [Cucumis melo])

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-57
Identity = 116/165 (70.30%), Postives = 134/165 (81.21%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           MAYMTH+ TELGIKP P++A FSSRGPNT+E  ILK    APGVNI+AA+SE  S +G  
Sbjct: 486 MAYMTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSL 545

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            D RR+ F  +SGTSMSCPHISGIVGLLKTLYP WSP AIKS IMTTA   ANDL+PI++
Sbjct: 546 FDNRRIPFNIVSGTSMSCPHISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILN 605

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
           +   KANP AYGAGHVQPN+A NPGLVYDL+T +Y+NFLCA+GYN
Sbjct: 606 TVNLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYN 650

[Close]

BLAST of CmaCh16G010010 vs. NCBI nr
Match: gi|778688353|ref|XP_011652727.1| (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus])

HSP 1 Score: 228.8 bits (582), Expect = 7.2e-57
Identity = 116/165 (70.30%), Postives = 134/165 (81.21%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           MAYMTH+ TELGIKP P++A FSSRGPNTVE  ILK    APGVNI+AA+SE  S +G  
Sbjct: 458 MAYMTHVRTELGIKPAPVMASFSSRGPNTVEETILKPDITAPGVNILAAYSEDASPSGSF 517

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            DKRR+ F  +SGTSMSCPHISGIVGLLKTLYP WSP AIKS IMTTA + AND++PI +
Sbjct: 518 FDKRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAESRANDMHPIQN 577

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
               KAN  AYGAGHVQPN+A NPGLVYDL+T++Y+NFLCA+GYN
Sbjct: 578 GGNLKANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLCAQGYN 622

[Close]

BLAST of CmaCh16G010010 vs. NCBI nr
Match: gi|700200864|gb|KGN55997.1| (hypothetical protein Csa_3G045110 [Cucumis sativus])

HSP 1 Score: 228.8 bits (582), Expect = 7.2e-57
Identity = 116/165 (70.30%), Postives = 134/165 (81.21%), Query Frame = 1


Query: 1   MAYMTHM-TELGIKPTPIIAPFSSRGPNTVEPLILK---AAPGVNIIAAFSEATSITGLP 60
           MAYMTH+ TELGIKP P++A FSSRGPNTVE  ILK    APGVNI+AA+SE  S +G  
Sbjct: 347 MAYMTHVRTELGIKPAPVMASFSSRGPNTVEETILKPDITAPGVNILAAYSEDASPSGSF 406

Query: 61  CDKRRVQFIELSGTSMSCPHISGIVGLLKTLYPKWSPTAIKSTIMTTAVAEANDLNPIIS 120
            DKRR+ F  +SGTSMSCPHISGIVGLLKTLYP WSP AIKS IMTTA + AND++PI +
Sbjct: 407 FDKRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAESRANDMHPIQN 466

Query: 121 SEKEKANPLAYGAGHVQPNKAANPGLVYDLSTQNYLNFLCARGYN 162
               KAN  AYGAGHVQPN+A NPGLVYDL+T++Y+NFLCA+GYN
Sbjct: 467 GGNLKANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLCAQGYN 511

The following BLAST results are available for this feature:

BLAST of CmaCh16G010010 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SBT54_ARATH	1.0e-50	64.74	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1	[more]
AIR3_ARATH	4.3e-46	59.24	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1	[more]
SBT18_ARATH	2.0e-43	56.05	Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1	[more]
SBT17_ARATH	3.4e-43	53.46	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1	[more]
SBT1_SOYBN	2.9e-42	57.76	Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3	[more]

BLAST of CmaCh16G010010 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2R9_CUCSA	3.5e-58	70.91	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1	[more]
A0A0A0L613_CUCSA	5.0e-57	70.30	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045110 PE=4 SV=1	[more]
W9QR32_9ROSA	3.7e-52	62.64	Subtilisin-like protease OS=Morus notabilis GN=L484_023738 PE=4 SV=1	[more]
W9R162_9ROSA	7.0e-51	61.82	Subtilisin-like protease OS=Morus notabilis GN=L484_023751 PE=4 SV=1	[more]
A0A151SLF4_CAJCA	2.0e-50	60.84	Subtilisin-like protease OS=Cajanus cajan GN=KK1_001840 PE=4 SV=1	[more]

BLAST of CmaCh16G010010 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G59810.1	5.6e-52	64.74	Subtilase family protein	[more]
AT2G04160.1	2.5e-47	59.24	Subtilisin-like serine endopeptidase family protein	[more]
AT2G05920.1	1.1e-44	56.05	Subtilase family protein	[more]
AT5G67360.1	1.9e-44	53.46	Subtilase family protein	[more]
AT4G34980.1	7.6e-41	49.70	subtilisin-like serine protease 2	[more]

BLAST of CmaCh16G010010 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778675733\|ref\|XP_011650462.1\|	5.0e-58	70.91	PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]
gi\|700200865\|gb\|KGN55998.1\|	5.0e-58	70.91	hypothetical protein Csa_3G045120 [Cucumis sativus]	[more]
gi\|659096606\|ref\|XP_008449186.1\|	1.1e-57	70.30	PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103491136 [Cucumis me...	[more]
gi\|778688353\|ref\|XP_011652727.1\|	7.2e-57	70.30	PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus]	[more]
gi\|700200864\|gb\|KGN55997.1\|	7.2e-57	70.30	hypothetical protein Csa_3G045110 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000209	Peptidase_S8/S53_dom
IPR015500	Peptidase_S8_subtilisin-rel
IPR023828	Peptidase_S8_Ser-AS

Vocabulary: **Molecular Function**
Term	Definition
GO:0004252	serine-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004252	serine-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G010010.1	CmaCh16G010010.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53 domain	GENE3D	G3DSA:3.40.50.200		coord: 14..152 score: 1.3
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 17..130 score: 2.4
IPR000209	Peptidase S8/S53 domain	unknown	SSF52743	Subtilisin-like	coord: 16..165 score: 3.01
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 1..163 score: 5.7
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 69..79 scor
None	No IPR available	PANTHER	PTHR10795:SF3	SUBFAMILY NOT NAMED	coord: 1..163 score: 5.7

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh16G010010	Cucumber (Chinese Long) v3	cmacucB0381
CmaCh16G010010	Watermelon (97103) v2	cmawmbB338
CmaCh16G010010	Wax gourd	cmawgoB0419
CmaCh16G010010	Cucurbita maxima (Rimu)	cmacmaB354
CmaCh16G010010	Cucumber (Gy14) v1	cgycmaB0029
CmaCh16G010010	Cucurbita moschata (Rifu)	cmacmoB323
CmaCh16G010010	Cucurbita moschata (Rifu)	cmacmoB341
CmaCh16G010010	Wild cucumber (PI 183967)	cmacpiB333
CmaCh16G010010	Cucumber (Chinese Long) v2	cmacuB327
CmaCh16G010010	Melon (DHL92) v3.5.1	cmameB290
CmaCh16G010010	Watermelon (Charleston Gray)	cmawcgB277
CmaCh16G010010	Watermelon (Charleston Gray)	cmawcgB285
CmaCh16G010010	Watermelon (97103) v1	cmawmB294
CmaCh16G010010	Cucurbita pepo (Zucchini)	cmacpeB345
CmaCh16G010010	Cucurbita pepo (Zucchini)	cmacpeB365
CmaCh16G010010	Bottle gourd (USVL1VR-Ls)	cmalsiB327
CmaCh16G010010	Cucumber (Gy14) v2	cgybcmaB340
CmaCh16G010010	Melon (DHL92) v3.6.1	cmamedB343
CmaCh16G010010	Silver-seed gourd	carcmaB0024
CmaCh16G010010	Silver-seed gourd	carcmaB1201