CmaCh16G009990 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G009990
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionSubtilisin-like serine protease
LocationCma_Chr16 : 7698565 .. 7703232 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCCTTCAATCTTCCTCCTTTGCTTCTCTCGTTCTTTCTTTTTGCTCTTTTGCAAACATCCACCCTTGCAGCCAAGAAGGTTAATCATCACTTATAAATCCTTTTTGATGTTTATATCGAGTGGGTTTTAGACATTGAAACGTTTTTTTTTTTTAATTTTGTACAGTCTTATATTGTTTATTTGGGATCACATTCGCATGGGTTGAATCCTTCTGCTGTTGATCTCCAACTTGCAACACAAACCCACTACACTCTACTTGAATCCGTGTTAGGAAGGTGAAACTTTTTGTTTCAAATCATGTATGGTAGATTTGTTGAACTCTCACTCTTCATTTACGCTCTTATATTTTTGTTTCTAAGTACAGCTATGAAGCAGCTAAGGAAGCAATCTTTTACTCATACAATAGACATATCAATGCCTTTGCAGCTGTTCTTGATCAAAAGTTGTAGAAGACATAGCAAGTAAAATGATTCATCCGTTTCTATGTCTATAGCTATCATTTGGTTTGATAATGAAACTTCCTAATAAAATCAAAAACAAATGATTCATCGTTTGTTCCTAATTTATTTAGTTTCATAGTTTTATGGAATTTCAAAAACAAAAACAACCCTTTTTAGATTCCATAACTTTGTAATGGGGAAGTATTCACAGACTTAATTTCAAAAGTCAAATAGTTATCAAATAGTTATCAAGCGAGCCTCATTTTTTAATTTAATAATGATGATGTTTTTATCATTTTCTGTGTTAGAACATCCTGATGTGATATCAGTGTATGAAAACAAAGAACTAAAACTGCACACGACACGATCATGGAACTTTCTTGGAGTTGAGAATAATGATATAATTCCTTCAAACTCGCTTTGGAAACTTTCAAGATTTGGTGAATCTACAATCATTGGCAACCTTGACTCGGGTTGGTCTTACTTCCTTTTTTAAGTTCTTTTTTTTTTTTTTAGTTTTTGTAATAATTTTTATATGTAATTGGAATCAATTTAGGTGTTTGGCCAGAATCAAAGAGTTTTAGTGATGAAGGATATGGACCTATCCCAACAAGATGGAAGGGAAGTTGTGAAGGTGGCTCCAACTTTAGTTGCAACAGGTACTTACCATCGAATCTCTTATTATTACTTCACCTCTATTTAATTCTTTTATGCTAACAAATATGCACATTTTCCATATTAGACACTTTTGTTAGCCTAATAGACAAATATTGAACAATAGTCGGTGTATAATTCGCGAATATTAATGTAAAATTTATTAGAGGCATATTGAATCATTGTTAATGATACTAAATAGACATGTACATGCATAAATTTTCATTTTCTTAGTTAAATTACAAGTATAGCTCCTAAACTTTCACGTTTATGTTTAAGATATCTTTGAATTTCAAAAATTATCTAATAAGGATTTAAACTTTTATTTTTTATGTGTCTAAAATATTTTTCAAATGTGTAATAGATTTGAACATTCAAGTATGTGTCTAATATGTGAGACATATTCAGATATTTAAAAACTAATTGGTCAAATTTTCTATCTATTAAAACTCTTAACCTTCCATTTATTAAAATTACAAGATGTAAAGTCTTTTTAAAGTTTTGGAATAAAATATAGATAGACCTATCATACATTTCTTTTAGTTCAAATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTGTAGTTTAATTTATGTTTTTAAGCATACAAAGACATAAATTCATCGACTTTTGAATTTTCTTTGTATATAAAAATAAGAGTAGACGTAATGCTTGTATCAGTACGTGTGACAATTTTAAATGCAGGAAGCTGATTGGAGCACGGTATTTCAACAAAGGTTATGCATCCAACGTGGGACCTCTCAACTCGAGCTATGAAACGGCAAGGGACGATGATGGGCATGGAACACACACCTTATCCACAGCTGGAGGCAATTTCGTTCAAAGAGTAAGCATTTTTGGGAATGGTTATGGCACGGCAAAAGGAGGTTCCCCTAAAGCTCGTGTTGCTGCCTATAGAGTATGTTGGCCTGAGGTGGCGAATGGTGGGTGTTTTATGGCCGATATTCTAGCTGGCTTTGAAGCTGCCATTAGCGATGGAGTTGACGTTCTATCGATTTCACTCGGTGGAGGTGCTATAGAATTTTCTGAAGATCTACTAGCTATAATGTCCTACCATACAGTGAAGAACGACATCACTGTTGTTTGTTCGGCTGGAAATTCAGGACCATTTGCAGGTACTGTCTCAAATGTCGCACCATGGATGATAACGGTGGGAGCTAGCACAGTTGACAGGCTTTTTACAAGTTATGTGGCGTTGGGAGACAAGAGGCACTTCAAGGTACTCTACTCAACACTTGTTAGTGCTATATGGTTGTGTTCTTGACAATATTTATATAAATATTTAGGTTAGTATCGAACAATTTTTAAACATAGTAAATTGTATTGGGCATATGAAGTTTTATCATATACATGTCTCTATATTTGTATCGATTGAGTTTATGGCTCCGTACTACGTTTATATTCTCACCAATGATTTTTCATTGTCAATATCAGGGTAAAAGTCTTTCTAGTAAATTATTGCCAACTAAGAAACTCTATCCGTTGATCCGTGCTTTAGATGCAAAATCCAACAATGTCTCTGATAACTACGCGTGAGTATATTTTTATTTAAATTATACAAAATATCTCTAAATTTTAGAAACATTTCAATCCTGCCCTCACCATTAACACTTCAATTTTTGAAACAGATCCAAAAAGAATTTTCTATTGAAACAATGTAAATCATTAAATGATGTTACCTATATCTTTTGAGTTGAACTAAGCAGTAAACGTTAATCATATTTTTTTTATATAGTTATAAGTTGTGTTATTATTATAATTTTTTCTAATTTTTAAGAATGTTGAACTTAATTTGATGGGTATTTTTCAAAATTTTTGCGAGAGTTAAATTTTAAAACATATTTTACATTTTAAAAGAGTATTTTATATAATTTAATCGGTAGGTTTTCACCTAATAATAAGAAGAAATTGCCAATAGCACATGATATTAAGCAAAATTTATTGTAATCTTGTAGCATACTATGTCGCAAAGGGTCTCTTGATCCTAAAAAGGTAAAAGGAAAAATTGTAGTTTGCCTTAGAGGGGACAATGCAAGAATCGAGAAAGGTTATGTGGTTGCTCAAGCAGGTGCTGTTGGGATGATTCTTGCTAATGACAAGGAGAATGGAGATGCATTCGAGGCTGATGCGCATTTACTTCCTGCTTCACATATAAGCTATACCGACGGTGAATCAGTCTACCAATACATCCAGTCTACTAAGTAATTTCTCCACCTTATTCCACAACTTCCATGTTTATTCAAAAAGTATTTTAAACTTTATGATCTAACCAACATAAATTTAGAAGTTTATTAATCTATTAAATGTACTTTTTAAGTTAAAAAATTATTAAACTCAAAATTTGTAACTTATATGTCATGTTTTGTTGTTGAAACAGAACTCCAATGGCTTACATGACTCACATGACTGAGTTGGGAATCAAACCCGCACCAATTATGGCTTCATTCTCATCAAGAGGTCCCAACACAATTGAGCCCTCAATACTCAAGGTTGGTTAAGAACGATGTGTTGAGTACTTGTACCGTATAAAGTTCAATGTGTCATCCCCTAACAATCTCAAAATTTTAAGTACTGCATTTTTTTTTTTAATTTCAGCCTGACATAATAGCACCAGGTGTAAATATAATAGCGGCTTTCTCTGAAGCAACATCCATAACTGGTTTACCTTATGATAAGCGTATGGTTCAATTCACTGAATTATCTGGCACTTCTATGTCATGCCCCCATATTTCTGGCATTGTTGGTCTTCTCAAAACTCTTTATCCAAAATGGAGTCCAGCGGCTATCAGGTCTGCAATCATGACCACAGGTTAGTTATTCAAAATTCTTCAACCATTTTAAAAGTTAACTGAGTAAATAAATTAGTAATTTATGAATAGTAAGAGATGTTATTATGGTATTTGCATTATTTTGTATTAAGAAACATTTTGACTTAAATCTGGCTAACTAATGATTTTGTTTGGTAGCTGGAACGGAAGCCAATGACTTAAATCCAATACTAAGCTCAGAAAAGGAGAAAGCAAACCCATTGGCATATGGTGCAGGCCATGTCCAACCAAATAAAGCAGCAAATCCTGGCCTTGTTTACGATCTCTCCATCCAAGACTACTTGAACTTCTTATGTGCTCGTGGCTACAATAAAGCACAAATGAAACTATTCACTAATGATACTTCATTTGTTTGTTCAAAGTCATTCAAAGTAATAGACTTGAATTATCCATCAATCTCGATATATAATCTAAAATCAGAGGTAGTGAATATCAAAAGAAGAGTGAAGAATGTAGGAAGTCCAGGCACCTATGTTGCTAAAGTCGAGTCACCGCCGGGAGTTTCAGTTTTGGTAGCACCAAGTACTTTGAAGTTCACTAAAACGGGTGAAGAGAAGAATTTCAAAGTTGTGTTGAGGAGGGTGCAAAGTAATCAAACTGAAGAGCATGTGTTCGGAAAACTTGTGTGGTCTGATGGGAAACATCGTGTTAGTAGTCCAATTTTTGTGACATTAGAGATTTAG

mRNA sequence

ATGGAGGCCTTCAATCTTCCTCCTTTGCTTCTCTCGTTCTTTCTTTTTGCTCTTTTGCAAACATCCACCCTTGCAGCCAAGAAGTCTTATATTGTTTATTTGGGATCACATTCGCATGGGTTGAATCCTTCTGCTGTTGATCTCCAACTTGCAACACAAACCCACTACACTCTACTTGAATCCGTGTTAGGAAGGAAGCTGATTGGAGCACGGTATTTCAACAAAGGTTATGCATCCAACGTGGGACCTCTCAACTCGAGCTATGAAACGGCAAGGGACGATGATGGGCATGGAACACACACCTTATCCACAGCTGGAGGCAATTTCGTTCAAAGAGTAAGCATTTTTGGGAATGGTTATGGCACGGCAAAAGGAGGTTCCCCTAAAGCTCGTGTTGCTGCCTATAGAGTATGTTGGCCTGAGGTGGCGAATGGTGGGTGTTTTATGGCCGATATTCTAGCTGGCTTTGAAGCTGCCATTAGCGATGGAGTTGACGTTCTATCGATTTCACTCGGTGGAGGTGCTATAGAATTTTCTGAAGATCTACTAGCTATAATGTCCTACCATACAGTGAAGAACGACATCACTGTTGTTTGTTCGGCTGGAAATTCAGGACCATTTGCAGGTACTGTCTCAAATGTCGCACCATGGATGATAACGGTGGGAGCTAGCACAGTTGACAGGCTTTTTACAAGTTATGTGGCGTTGGGAGACAAGAGGCACTTCAAGGGTAAAAGTCTTTCTAGTAAATTATTGCCAACTAAGAAACTCTATCCGTTGATCCGTGCTTTAGATGCAAAATCCAACAATGTCTCTGATAACTACGCCATACTATGTCGCAAAGGGTCTCTTGATCCTAAAAAGGTAAAAGGAAAAATTGTAGTTTGCCTTAGAGGGGACAATGCAAGAATCGAGAAAGGTTATGTGGTTGCTCAAGCAGGTGCTGTTGGGATGATTCTTGCTAATGACAAGGAGAATGGAGATGCATTCGAGGCTGATGCGCATTTACTTCCTGCTTCACATATAAGCTATACCGACGGTGAATCAGTCTACCAATACATCCAGTCTACTAAAACTCCAATGGCTTACATGACTCACATGACTGAGTTGGGAATCAAACCCGCACCAATTATGGCTTCATTCTCATCAAGAGGTCCCAACACAATTGAGCCCTCAATACTCAAGCCTGACATAATAGCACCAGGTGTAAATATAATAGCGGCTTTCTCTGAAGCAACATCCATAACTGGTTTACCTTATGATAAGCGTATGGTTCAATTCACTGAATTATCTGGCACTTCTATGTCATGCCCCCATATTTCTGGCATTGTTGGTCTTCTCAAAACTCTTTATCCAAAATGGAGTCCAGCGGCTATCAGGTCTGCAATCATGACCACAGCTGGAACGGAAGCCAATGACTTAAATCCAATACTAAGCTCAGAAAAGGAGAAAGCAAACCCATTGGCATATGGTGCAGGCCATGTCCAACCAAATAAAGCAGCAAATCCTGGCCTTGTTTACGATCTCTCCATCCAAGACTACTTGAACTTCTTATGTGCTCGTGGCTACAATAAAGCACAAATGAAACTATTCACTAATGATACTTCATTTGTTTGTTCAAAGTCATTCAAAGTAATAGACTTGAATTATCCATCAATCTCGATATATAATCTAAAATCAGAGGTAGTGAATATCAAAAGAAGAGTGAAGAATGTAGGAAGTCCAGGCACCTATGTTGCTAAAGTCGAGTCACCGCCGGGAGTTTCAGTTTTGGTAGCACCAAGTACTTTGAAGTTCACTAAAACGGGTGAAGAGAAGAATTTCAAAGTTGTGTTGAGGAGGGTGCAAAGTAATCAAACTGAAGAGCATGTGTTCGGAAAACTTGTGTGGTCTGATGGGAAACATCGTGTTAGTAGTCCAATTTTTGTGACATTAGAGATTTAG

Coding sequence (CDS)

ATGGAGGCCTTCAATCTTCCTCCTTTGCTTCTCTCGTTCTTTCTTTTTGCTCTTTTGCAAACATCCACCCTTGCAGCCAAGAAGTCTTATATTGTTTATTTGGGATCACATTCGCATGGGTTGAATCCTTCTGCTGTTGATCTCCAACTTGCAACACAAACCCACTACACTCTACTTGAATCCGTGTTAGGAAGGAAGCTGATTGGAGCACGGTATTTCAACAAAGGTTATGCATCCAACGTGGGACCTCTCAACTCGAGCTATGAAACGGCAAGGGACGATGATGGGCATGGAACACACACCTTATCCACAGCTGGAGGCAATTTCGTTCAAAGAGTAAGCATTTTTGGGAATGGTTATGGCACGGCAAAAGGAGGTTCCCCTAAAGCTCGTGTTGCTGCCTATAGAGTATGTTGGCCTGAGGTGGCGAATGGTGGGTGTTTTATGGCCGATATTCTAGCTGGCTTTGAAGCTGCCATTAGCGATGGAGTTGACGTTCTATCGATTTCACTCGGTGGAGGTGCTATAGAATTTTCTGAAGATCTACTAGCTATAATGTCCTACCATACAGTGAAGAACGACATCACTGTTGTTTGTTCGGCTGGAAATTCAGGACCATTTGCAGGTACTGTCTCAAATGTCGCACCATGGATGATAACGGTGGGAGCTAGCACAGTTGACAGGCTTTTTACAAGTTATGTGGCGTTGGGAGACAAGAGGCACTTCAAGGGTAAAAGTCTTTCTAGTAAATTATTGCCAACTAAGAAACTCTATCCGTTGATCCGTGCTTTAGATGCAAAATCCAACAATGTCTCTGATAACTACGCCATACTATGTCGCAAAGGGTCTCTTGATCCTAAAAAGGTAAAAGGAAAAATTGTAGTTTGCCTTAGAGGGGACAATGCAAGAATCGAGAAAGGTTATGTGGTTGCTCAAGCAGGTGCTGTTGGGATGATTCTTGCTAATGACAAGGAGAATGGAGATGCATTCGAGGCTGATGCGCATTTACTTCCTGCTTCACATATAAGCTATACCGACGGTGAATCAGTCTACCAATACATCCAGTCTACTAAAACTCCAATGGCTTACATGACTCACATGACTGAGTTGGGAATCAAACCCGCACCAATTATGGCTTCATTCTCATCAAGAGGTCCCAACACAATTGAGCCCTCAATACTCAAGCCTGACATAATAGCACCAGGTGTAAATATAATAGCGGCTTTCTCTGAAGCAACATCCATAACTGGTTTACCTTATGATAAGCGTATGGTTCAATTCACTGAATTATCTGGCACTTCTATGTCATGCCCCCATATTTCTGGCATTGTTGGTCTTCTCAAAACTCTTTATCCAAAATGGAGTCCAGCGGCTATCAGGTCTGCAATCATGACCACAGCTGGAACGGAAGCCAATGACTTAAATCCAATACTAAGCTCAGAAAAGGAGAAAGCAAACCCATTGGCATATGGTGCAGGCCATGTCCAACCAAATAAAGCAGCAAATCCTGGCCTTGTTTACGATCTCTCCATCCAAGACTACTTGAACTTCTTATGTGCTCGTGGCTACAATAAAGCACAAATGAAACTATTCACTAATGATACTTCATTTGTTTGTTCAAAGTCATTCAAAGTAATAGACTTGAATTATCCATCAATCTCGATATATAATCTAAAATCAGAGGTAGTGAATATCAAAAGAAGAGTGAAGAATGTAGGAAGTCCAGGCACCTATGTTGCTAAAGTCGAGTCACCGCCGGGAGTTTCAGTTTTGGTAGCACCAAGTACTTTGAAGTTCACTAAAACGGGTGAAGAGAAGAATTTCAAAGTTGTGTTGAGGAGGGTGCAAAGTAATCAAACTGAAGAGCATGTGTTCGGAAAACTTGTGTGGTCTGATGGGAAACATCGTGTTAGTAGTCCAATTTTTGTGACATTAGAGATTTAG

Protein sequence

MEAFNLPPLLLSFFLFALLQTSTLAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKGKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYMTHMTELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTLEI
BLAST of CmaCh16G009990 vs. Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 5.3e-193
Identity = 353/583 (60.55%), Postives = 423/583 (72.56%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVG-PLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTA 124
           RKLIGARYFNKGY +  G P N+SYET RD DGHG+HTLSTA GNFV   ++FG G GTA
Sbjct: 197 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 256

Query: 125 KGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 184
            GGSPKARVAAY+VCWP V    CF ADILA  EAAI DGVDVLS S+GG A ++  D +
Sbjct: 257 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 316

Query: 185 AIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFK 244
           AI S+H VKN +TVVCSAGNSGP +GTVSNVAPW+ITVGAS++DR F ++V L + + FK
Sbjct: 317 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 376

Query: 245 GKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNAR 304
           G SLS K LP +K+Y LI A DA   N +   A+LC+KGSLDPKKVKGKI+VCLRGDNAR
Sbjct: 377 GTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNAR 436

Query: 305 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAY 364
           ++KG   A AGA GM+L NDK +G+   +DAH+LPAS I Y DGE+++ Y+ STK P  Y
Sbjct: 437 VDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGY 496

Query: 365 MTHMTE-LGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDK 424
           +   T  L  KPAP MASFSSRGPNTI P ILKPDI APGVNIIAAF+EAT  T L  D 
Sbjct: 497 IKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDN 556

Query: 425 RMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEK 484
           R   F   SGTSMSCPHISG+VGLLKTL+P WSPAAIRSAIMTT+ T  N   P++    
Sbjct: 557 RRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESF 616

Query: 485 EKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 544
           +KANP +YG+GHVQPNKAA+PGLVYDL+  DYL+FLCA GYN   ++LF  D  + C + 
Sbjct: 617 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 676

Query: 545 FKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKT 604
             ++D NYPSI++ NL    + + R++KNVG P TY A+   P GV V V P  L F KT
Sbjct: 677 ANLLDFNYPSITVPNLTGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 736

Query: 605 GEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
           GE K F++ LR +    +  +VFG+L W+D  H V SPI V L
Sbjct: 737 GEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of CmaCh16G009990 vs. Swiss-Prot
Match: AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.4e-190
Identity = 339/582 (58.25%), Postives = 422/582 (72.51%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARYFNKGYA+ VG LNSS+++ RD DGHG+HTLSTA G+FV  VSIFG G GTAK
Sbjct: 191 RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 125 GGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLA 184
           GGSP+ARVAAY+VCWP V    C+ AD+LA F+AAI DG DV+S+SLGG    F  D +A
Sbjct: 251 GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 185 IMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKG 244
           I S+H  K  I VVCSAGNSGP   TVSNVAPW ITVGAST+DR F S + LG+ +H+KG
Sbjct: 311 IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 245 KSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARI 304
           +SLSS  LP  K YP++ +++AK+ N S   A LC+ GSLDP K KGKI+VCLRG N R+
Sbjct: 371 QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 305 EKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYM 364
           EKG  VA  G +GM+L N    G+   AD H+LPA+ ++  D  +V +YI  TK P+A++
Sbjct: 431 EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 365 T-HMTELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKR 424
           T   T+LG+KPAP+MASFSS+GP+ + P ILKPDI APGV++IAA++ A S T   +D R
Sbjct: 491 TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 425 MVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKE 484
            + F  +SGTSMSCPHISGI GLLKT YP WSPAAIRSAIMTTA    +   PI ++   
Sbjct: 551 RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 485 KANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKSF 544
           KA P ++GAGHVQPN A NPGLVYDL I+DYLNFLC+ GYN +Q+ +F+ +     S   
Sbjct: 611 KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

Query: 545 KVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKTG 604
            +++LNYPSI++ NL S  V + R VKNVG P  Y  KV +P GV V V P++L FTK G
Sbjct: 671 SLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVG 730

Query: 605 EEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
           E+K FKV+L + + N  + +VFG+LVWSD KHRV SPI V L
Sbjct: 731 EQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CmaCh16G009990 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 5.7e-147
Identity = 297/634 (46.85%), Postives = 402/634 (63.41%), Query Frame = 1

Query: 18  LLQTSTLAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGY 77
           +L T      KSY         G  PS+        T++T   S+  RKLIGAR+F +GY
Sbjct: 137 VLDTGVWPESKSY----SDEGFGPIPSSWKGGCEAGTNFTA--SLCNRKLIGARFFARGY 196

Query: 78  ASNVGPLNSSYETA--RDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAY 137
            S +GP++ S E+   RDDDGHGTHT STA G+ V+  S+ G   GTA+G +P+ARVA Y
Sbjct: 197 ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVY 256

Query: 138 RVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDI 197
           +VCW     GGCF +DILA  + AI+D V+VLS+SLGGG  ++  D +AI ++  ++  I
Sbjct: 257 KVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGI 316

Query: 198 TVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKGKSLSSKLLPTK 257
            V CSAGN+GP + ++SNVAPW+ TVGA T+DR F +   LG+ ++F G SL        
Sbjct: 317 LVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD 376

Query: 258 KLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGA 317
           KL P I A +A SN  + N   LC  G+L P+KVKGKIV+C RG NAR++KG VV  AG 
Sbjct: 377 KLLPFIYAGNA-SNATNGN---LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGG 436

Query: 318 VGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYMTHM-TELGIKP 377
           VGMILAN   NG+   ADAHLLPA+ +    G+ +  Y+ +   P A ++ + T +G+KP
Sbjct: 437 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP 496

Query: 378 APIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKRMVQFTELSGTS 437
           +P++A+FSSRGPN+I P+ILKPD+IAPGVNI+AA++ A   TGL  D R V+F  +SGTS
Sbjct: 497 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTS 556

Query: 438 MSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKEK-ANPLAYGAG 497
           MSCPH+SG+  LLK+++P+WSPAAIRSA+MTTA     D  P+L     K + P  +GAG
Sbjct: 557 MSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAG 616

Query: 498 HVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVC--SKSFKVIDLNYP 557
           HV P  A NPGL+YDL+ +DYL FLCA  Y   Q++  +   ++ C  SKS+ V DLNYP
Sbjct: 617 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYP 676

Query: 558 SISIYNLKSEVVNIKRRVKNVGSPGTYVAKVES-PPGVSVLVAPSTLKFTKTGEEKNFKV 617
           S ++           R V +VG  GTY  KV S   GV + V P+ L F +  E+K++ V
Sbjct: 677 SFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 736

Query: 618 VLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVT 645
                 S  +  + FG + WSDGKH V SP+ ++
Sbjct: 737 TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmaCh16G009990 vs. Swiss-Prot
Match: SBT1_SOYBN (Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 515.8 bits (1327), Expect = 7.0e-145
Identity = 291/603 (48.26%), Postives = 380/603 (63.02%), Query Frame = 1

Query: 59  LESVLGRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGN 118
           +++   RKLIGARY+NK + ++ G L+    TARD  GHGTHTLSTAGGNFV    +F  
Sbjct: 186 MKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAV 245

Query: 119 GYGTAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 178
           G GTAKGGSP+ARVAAY+VCW       C+ AD+LA  + AI DGVDV+++S G   +  
Sbjct: 246 GNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVT 305

Query: 179 SE----DLLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYV 238
           +E    D ++I ++H +  +I +V SAGN GP  GTV+NVAPW+ T+ AST+DR F+S +
Sbjct: 306 AEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNL 365

Query: 239 ALGDKRHFKGKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIV 298
            + ++   +G SL   L P  + + LI + DAK  N +   A LCR+G+LD  KV GKIV
Sbjct: 366 TINNQL-IEGASLFVNL-PPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIV 425

Query: 299 VCLR-GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQY 358
           +C R G    + +G     AGA GMIL N  +NG    A+ H+    +      +S    
Sbjct: 426 LCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHG 485

Query: 359 IQST---------KT--PMAYMTHMTELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPG 418
           +++T         KT   +      T  G KPAP+MASFSSRGPN I+PSILKPD+ APG
Sbjct: 486 VKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPG 545

Query: 419 VNIIAAFSEATSITGLPYDKRM-VQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRS 478
           VNI+AA+SE  S + L  D R   +F  L GTSMSCPH SGI GLLKT +P WSPAAI+S
Sbjct: 546 VNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKS 605

Query: 479 AIMTTAGTEANDLNPILSS-EKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCA 538
           AIMTTA T  N   PI  + +K  A+  AYG+GHV+P+ A  PGLVYDLS+ DYLNFLCA
Sbjct: 606 AIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCA 665

Query: 539 RGYNKAQMKLFTNDTSFVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVA 598
            GY++  +     + +F+CS S  V DLNYPSI++ NL+ + V I R V NVG P TY  
Sbjct: 666 SGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTV 725

Query: 599 KVESPPGVSVLVAPSTLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSP 644
              SP G S+ V P +L FTK GE K FKV+++   +    ++ FG L W+DGKH V SP
Sbjct: 726 STRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSP 785

BLAST of CmaCh16G009990 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 6.1e-133
Identity = 270/586 (46.08%), Postives = 368/586 (62.80%), Query Frame = 1

Query: 65  RKLIGARYFNKGYA-SNVGPLNSSYETA--RDDDGHGTHTLSTAGGNFVQRVSIFGNGYG 124
           +KLIGAR F+KG+  ++ G  +S  E+   RD DGHGTHT +TA G+ V+  S  G   G
Sbjct: 173 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 232

Query: 125 TAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSED 184
           TA+G + +ARVA Y+VCW    + GCF +DILA  + AI DGVDVLS+SLGGG+  +  D
Sbjct: 233 TARGMATRARVATYKVCW----STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRD 292

Query: 185 LLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRH 244
            +AI ++  ++  + V CSAGNSGP   +V+NVAPW++TVGA T+DR F ++  LG+ + 
Sbjct: 293 TIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKR 352

Query: 245 FKGKSLSSKL-LPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGD 304
             G SL S + + TK L  +      K N+ S N   LC  GSLD   V+GKIVVC RG 
Sbjct: 353 LTGVSLYSGVGMGTKPLELVYN----KGNSSSSN---LCLPGSLDSSIVRGKIVVCDRGV 412

Query: 305 NARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTP 364
           NAR+EKG VV  AG +GMI+AN   +G+   AD+HLLPA  +    G+ + +Y++S   P
Sbjct: 413 NARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKP 472

Query: 365 MAYMTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLP 424
            A +    T L +KP+P++A+FSSRGPNT+ P ILKPD+I PGVNI+A +S+A   TGL 
Sbjct: 473 TALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLD 532

Query: 425 YDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPIL- 484
            D R  QF  +SGTSMSCPHISG+ GLLK  +P+WSP+AI+SA+MTTA    N   P+  
Sbjct: 533 KDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHD 592

Query: 485 SSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFV 544
           +++   +NP A+G+GHV P KA +PGLVYD+S ++Y+ FLC+  Y    +       S  
Sbjct: 593 AADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVN 652

Query: 545 CSKSFK-VIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGT-YVAKVESPPGVSVLVAPST 604
           CSK F     LNYPS S+      VV   R V NVG+  + Y   V   P V + V PS 
Sbjct: 653 CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 712

Query: 605 LKFTKTGEEKNFKVV-LRRVQSNQTEEHVFGKLVWSDGKHRVSSPI 642
           L F   GE+K + V  + +   + T +  FG + WS+ +H V SP+
Sbjct: 713 LSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPV 747

BLAST of CmaCh16G009990 vs. TrEMBL
Match: A0A0A0L2R9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 6.8e-240
Identity = 423/585 (72.31%), Postives = 494/585 (84.44%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARY+NKGYA+ VGPLNSSYE+ARD +GHGTHTLSTAGG+FV   ++FG G GTAK
Sbjct: 174 RKLIGARYYNKGYAAIVGPLNSSYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGTAK 233

Query: 125 GGSPKARVAAYRVCWPEVAN-GGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 184
           GGSPKA VAAY+VCWP+V   G CF ADILAGFEAAI DGVDVLS+SLGG   +F++D +
Sbjct: 234 GGSPKALVAAYKVCWPQVLFFGECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFAKDSI 293

Query: 185 AIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFK 244
           +I S+H V+N I VVCSAGNSGP  G+VSNVAPW+ITVGAST DRL+TSYVA+GDKRHFK
Sbjct: 294 SIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHFK 353

Query: 245 GKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNAR 304
           G S+S K LP +K YPLI +LDAK+ NV+DN A+LC +GSLDPKKV GKI++CLRGDNAR
Sbjct: 354 GASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRGDNAR 413

Query: 305 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAY 364
           + KGYV A+AGAVGMILAN +ENGD   ADAHLLPASHI+Y+DG+ VYQYI STK PMAY
Sbjct: 414 VAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKIPMAY 473

Query: 365 MTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDK 424
           MTH+ TE GIKPAP+MASFSSRGPNT++PSILKPDI APG NI+AA+S   S TG  +DK
Sbjct: 474 MTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGTDFDK 533

Query: 425 RMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEK 484
           R V F   SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIMTTAGT+ANDL PILS+ +
Sbjct: 534 RRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILSTNQ 593

Query: 485 EKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 544
           EKAN  AYGAGHV PN+AA+PGLVYDLS +DYLN+LCA+GYN AQ+K F+NDTSFVCSKS
Sbjct: 594 EKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFVCSKS 653

Query: 545 FKVIDLNYPSISIYNLKSEV-VNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTK 604
           FK+ DLNYPSISI  L+ +V V IKR++KNVGSPGTYV +V+ P GVSV V P++LKFT 
Sbjct: 654 FKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSLKFTG 713

Query: 605 TGEEKNFKVVLRRVQSNQT-EEHVFGKLVWSDGKHRVSSPIFVTL 646
             EEK+F+VVL+  + N +  +++FGKL WSDGKHRV SPI V L
Sbjct: 714 IDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDGKHRVRSPIVVRL 758

BLAST of CmaCh16G009990 vs. TrEMBL
Match: A0A0A0L613_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045110 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 9.6e-234
Identity = 410/606 (67.66%), Postives = 494/606 (81.52%), Query Frame = 1

Query: 42  NPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHT 101
           NPS +   ++   +  L++    RKLIGA+YFNKGYA+N G LN+SYETARD++GHGTHT
Sbjct: 29  NPSLLSFSISF--YIVLIKFQKYRKLIGAKYFNKGYAANAGSLNASYETARDNEGHGTHT 88

Query: 102 LSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAYRVCWPEV-ANGGCFMADILAGFEAAI 161
           LSTAGGNF+   ++FGNG GTAKGGSPKA VAAY+VCWP+V + GGC+ ADILA  EAAI
Sbjct: 89  LSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWPQVDSGGGCYDADILAAMEAAI 148

Query: 162 SDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMIT 221
           SDGVDVLS+SLGGG+ +FS+D+ AI ++H V+  I VVCSAGNSGP  GTV NVAPW+IT
Sbjct: 149 SDGVDVLSLSLGGGSKDFSDDVTAIGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIIT 208

Query: 222 VGASTVDRLFTSYVALGDKRHFKGKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCR 281
           VGAST++R FTS+V+LG+K+H KG SLS K+LP +K YPLI A+DAK+N VS + A LC 
Sbjct: 209 VGASTINRDFTSFVSLGNKKHIKGASLSDKILPEQKFYPLIDAVDAKANKVSSDIAQLCL 268

Query: 282 KGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPAS 341
            GSLDP+KVKGKI++CLRG+NAR +KGY   +AGAVGMILAN +ENGD   ADAHLLP S
Sbjct: 269 VGSLDPEKVKGKIIICLRGENARADKGYAAVKAGAVGMILANAEENGDEIIADAHLLPVS 328

Query: 342 HISYTDGESVYQYIQSTKTPMAYMTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDII 401
           H+SYTDG+S+YQYI STKTPMAYMTH+ TELGIKPAP+MASFSSRGPNT+E +ILKPDI 
Sbjct: 329 HVSYTDGQSIYQYINSTKTPMAYMTHVRTELGIKPAPVMASFSSRGPNTVEETILKPDIT 388

Query: 402 APGVNIIAAFSEATSITGLPYDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAI 461
           APGVNI+AA+SE  S +G  +DKR + F  +SGTSMSCPHISGIVGLLKTLYP WSPAAI
Sbjct: 389 APGVNILAAYSEDASPSGSFFDKRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAI 448

Query: 462 RSAIMTTAGTEANDLNPILSSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLC 521
           +SAIMTTA + AND++PI +    KAN  AYGAGHVQPN+A NPGLVYDL+ +DY+NFLC
Sbjct: 449 KSAIMTTAESRANDMHPIQNGGNLKANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLC 508

Query: 522 ARGYNKAQMKLFTNDTSFVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYV 581
           A+GYNK+Q+  F +  SFVCSKSFK+ D NYPSISI ++KS VV IKRRVKNVG P TYV
Sbjct: 509 AQGYNKSQISKF-SAMSFVCSKSFKLTDFNYPSISIPDMKSGVVTIKRRVKNVGKPSTYV 568

Query: 582 AKVESPPGVSVLVAPSTLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSS 641
           A+V+ P GVSV V P TLKFT+  EEK+FKVV+  V +N+ + +VFG L+W DGKH V S
Sbjct: 569 ARVKVPHGVSVSVEPRTLKFTRIDEEKSFKVVIGSVANNKHKGYVFGSLIWEDGKHHVRS 628

Query: 642 PIFVTL 646
           PI V L
Sbjct: 629 PIVVNL 631

BLAST of CmaCh16G009990 vs. TrEMBL
Match: B9N5M9_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16870g PE=4 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 8.2e-209
Identity = 374/582 (64.26%), Postives = 448/582 (76.98%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARYFNKGY S  G LNSS++TARD +GHGTHTLSTA GNFV   ++FGNG GTAK
Sbjct: 190 RKLIGARYFNKGYGSIGGHLNSSFQTARDIEGHGTHTLSTAAGNFVPGANVFGNGKGTAK 249

Query: 125 GGSPKARVAAYRVCWPEVA--NGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDL 184
           GGSP+ARVAAY+VCWP V    GGC+ ADILAGF+ AISDGVDVLS+SLGG   E+S+D 
Sbjct: 250 GGSPRARVAAYKVCWPAVGVNEGGCYEADILAGFDVAISDGVDVLSVSLGGAIDEYSDDA 309

Query: 185 LAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHF 244
           +AI S+H  K  ITVV SAGNSGP  G+VSNVAPW+ITVGAST+DR FT YVALG+++H 
Sbjct: 310 IAIGSFHAFKKGITVVASAGNSGPGPGSVSNVAPWLITVGASTLDRAFTIYVALGNRKHL 369

Query: 245 KGKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNA 304
           KG SLS K LP +K YPLI    AK++N S+  A LC+ G+LD KKVKGKI+VCLRG N 
Sbjct: 370 KGVSLSQKSLPARKFYPLISGARAKASNQSEEDANLCKPGTLDSKKVKGKILVCLRGVNP 429

Query: 305 RIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMA 364
           R+EKG+V   AGAVGMILAND+E+G+   ADAH+LPA+HI  TDG++V+ Y+ STK P A
Sbjct: 430 RVEKGHVALLAGAVGMILANDEESGNGILADAHVLPAAHIISTDGQAVFSYLNSTKDPWA 489

Query: 365 YMTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYD 424
           Y+T++ TELG KPAP MASFSSRGPN +E SILKPDI APGV++IAAF+ AT  T   YD
Sbjct: 490 YITNVRTELGTKPAPFMASFSSRGPNILEESILKPDITAPGVSVIAAFTLATGPTDTAYD 549

Query: 425 KRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSE 484
           KR + F   SGTSMSCPH+SGIVGLLK+L+P WSPAAIRSAIMTTA T  N+ +PIL S 
Sbjct: 550 KRRIPFNTESGTSMSCPHVSGIVGLLKSLHPDWSPAAIRSAIMTTATTRDNNGDPILDSS 609

Query: 485 KEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSK 544
             +A P AYGAGHVQPN+AA+PGLVYDL++ D+LN+LC+RGY    +KLFT D  + C K
Sbjct: 610 NTRATPFAYGAGHVQPNRAADPGLVYDLTVNDFLNYLCSRGYTAKDLKLFT-DKPYTCPK 669

Query: 545 SFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTK 604
           SF + D NYPSIS  NL ++ + + RRVKNVGSPG Y   V  P GV V VAP+TL+F K
Sbjct: 670 SFSLTDFNYPSISAINL-NDTITVTRRVKNVGSPGKYYIHVREPTGVLVSVAPTTLEFKK 729

Query: 605 TGEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFV 644
            GEEK FKV  +     + +++ FG L WSDGKH V SP+ V
Sbjct: 730 LGEEKTFKVTFKLAPKWKLKDYTFGILTWSDGKHFVRSPLVV 769

BLAST of CmaCh16G009990 vs. TrEMBL
Match: F6I593_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g01420 PE=4 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 4.9e-206
Identity = 363/580 (62.59%), Postives = 453/580 (78.10%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARYFNKGYA+  GPLNSS+ +ARD +GHG+HTLSTAGG+ V   S+FG G GTAK
Sbjct: 189 RKLIGARYFNKGYAAYAGPLNSSFNSARDHEGHGSHTLSTAGGSLVYGASVFGYGNGTAK 248

Query: 125 GGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLA 184
           GGSP ARVAAY+VCWP+V NGGCF ADI+A F+AAI DGVDVLS+SLGG A ++  D LA
Sbjct: 249 GGSPGARVAAYKVCWPQVNNGGCFDADIMAAFDAAIHDGVDVLSVSLGGDASDYFTDGLA 308

Query: 185 IMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKG 244
           I S+H VK  I VV SAGN GP   +VSNV+PWMITVGAST+DR FT+YVALG+++H KG
Sbjct: 309 IGSFHAVKRGIVVVSSAGNDGPKDASVSNVSPWMITVGASTIDREFTNYVALGNRKHLKG 368

Query: 245 KSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARI 304
            SLS+K LP+ K YP+I +LDAK+ N S   AILC+ G+L+PKKVKGKI+VCLRG+N R+
Sbjct: 369 MSLSTKGLPSNKFYPVISSLDAKAANASAQDAILCKPGTLNPKKVKGKILVCLRGENPRV 428

Query: 305 EKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYM 364
           +KG   A AGAVG ILAND ++G+   AD H+LPASH++++DG +V+ YI STK PMAY+
Sbjct: 429 DKGEQAALAGAVGFILANDMQSGNELIADPHVLPASHVNFSDGAAVFNYINSTKNPMAYL 488

Query: 365 THM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKR 424
           T + T+LGIKPAP MASFSS+GPNTI P ILKPDI APGVNIIAA+SE+   T   +DKR
Sbjct: 489 TRVRTQLGIKPAPFMASFSSKGPNTITPEILKPDITAPGVNIIAAYSESIGPTDQTFDKR 548

Query: 425 MVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKE 484
            + F   SGTSMSCPHISGIVGLLKTL+P WSPAAI+SAIMT+A T  +++ P+L+S   
Sbjct: 549 RIPFNAQSGTSMSCPHISGIVGLLKTLHPDWSPAAIKSAIMTSARTRDDNMEPMLNSSNL 608

Query: 485 KANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKSF 544
           KA P +YGAGHV+PN+A +PGLVYD ++ DYLNFLCA GYN+ Q+++F +   + C KSF
Sbjct: 609 KATPFSYGAGHVRPNRAMDPGLVYDSTVNDYLNFLCAIGYNETQLQIF-SQKPYKCPKSF 668

Query: 545 KVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKTG 604
            +   NYPSI+  NL S  V I R VKNVG+PGTY A V++PPG+SV V P+ L+F + G
Sbjct: 669 SLTGFNYPSITAPNL-SGSVTISRTVKNVGTPGTYTASVKAPPGISVAVKPNKLEFREYG 728

Query: 605 EEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFV 644
           EEK+F++ L+       E++VFG+L+WSDG+H V S I V
Sbjct: 729 EEKSFRLTLKAKGRRVAEDYVFGRLIWSDGQHYVRSSIVV 766

BLAST of CmaCh16G009990 vs. TrEMBL
Match: W9QR32_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_023738 PE=4 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 6.5e-206
Identity = 372/590 (63.05%), Postives = 451/590 (76.44%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLN--------SSYETARDDDGHGTHTLSTAGGNFVQRVSIF 124
           RKLIGARYFN+GYA+ +  +N        S+  + RD +GHGTHTLSTAGG+FV   ++F
Sbjct: 141 RKLIGARYFNRGYAAYLLTMNTTITPTENSTLFSIRDHEGHGTHTLSTAGGSFVTDATVF 200

Query: 125 GNGYGTAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAI 184
           GNG GTAKGGSP+ARVAAY+VCWP V    CF ADI+A FE AI+DG+DVLS+SLGG   
Sbjct: 201 GNGNGTAKGGSPRARVAAYKVCWPPVNGNECFDADIIAAFETAITDGIDVLSVSLGGEPS 260

Query: 185 EFSEDLLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVAL 244
           EF ED ++I ++H VKN I VV SAGNSGP  GTVSNV+PWM+TVGAST+DR F+SYVAL
Sbjct: 261 EFFEDGISIGAFHAVKNGIVVVSSAGNSGPTPGTVSNVSPWMVTVGASTIDREFSSYVAL 320

Query: 245 GDKRHFKGKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVC 304
           G K+H KG SLSSK+LP++K YPLI   +A++ NV  + A+LC+ G+LDPKKVKGKIVVC
Sbjct: 321 GSKKHLKGASLSSKVLPSEKFYPLISGANARAANVPPSEALLCKNGTLDPKKVKGKIVVC 380

Query: 305 LRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQS 364
           LRGD AR EKG   + AGAVGMILAND ++G+   ADAHLLPASHI++TDG SV+ Y+ S
Sbjct: 381 LRGDIARTEKGQYASLAGAVGMILANDLQSGNEIIADAHLLPASHINFTDGNSVFAYLNS 440

Query: 365 TKTPMAYMTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSI 424
           TKTPMAYMT + TE+GIKPAP MASFSSRGPNTIE ++LKPDI APGVNIIAA+++A   
Sbjct: 441 TKTPMAYMTRVKTEVGIKPAPFMASFSSRGPNTIEEALLKPDITAPGVNIIAAYTQAAGP 500

Query: 425 TGLPYDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLN 484
           T   +DKR V F   SGTSMSCPH+SGIVGLLKTL+P WSPAAI+SAIMT+A T  ++  
Sbjct: 501 TDQDFDKRRVPFNTESGTSMSCPHVSGIVGLLKTLHPDWSPAAIKSAIMTSARTRDDNKE 560

Query: 485 PILSSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDT 544
           P+L S  +KA P AYGAGH+QPN A +PGLVYDL+  DYLNFLCARGYN+  +KLF +D 
Sbjct: 561 PLLDSSMKKATPFAYGAGHIQPNHAMDPGLVYDLTTDDYLNFLCARGYNETLLKLF-SDK 620

Query: 545 SFVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPS 604
              C KSF + + NYPSI + NL SE + + RRVKNVG+PG Y   V+ P GVSV V P 
Sbjct: 621 PHNCPKSFTLANFNYPSIVVPNLGSESIVVSRRVKNVGTPGIYTVYVKQPAGVSVSVWPK 680

Query: 605 TLKFTKTGEEKNFKVVLRR--VQSNQTEEHVFGKLVWSDGKHRVSSPIFV 644
           +LKF+K GEEK F V ++   V  +  + +VFG L WSDGKH V SPI V
Sbjct: 681 SLKFSKIGEEKKFNVFVKTKGVGKSTKDGYVFGHLKWSDGKHNVRSPIVV 729

BLAST of CmaCh16G009990 vs. TAIR10
Match: AT5G59810.1 (AT5G59810.1 Subtilase family protein)

HSP 1 Score: 675.6 bits (1742), Expect = 3.0e-194
Identity = 353/583 (60.55%), Postives = 423/583 (72.56%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVG-PLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTA 124
           RKLIGARYFNKGY +  G P N+SYET RD DGHG+HTLSTA GNFV   ++FG G GTA
Sbjct: 197 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 256

Query: 125 KGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 184
            GGSPKARVAAY+VCWP V    CF ADILA  EAAI DGVDVLS S+GG A ++  D +
Sbjct: 257 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 316

Query: 185 AIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFK 244
           AI S+H VKN +TVVCSAGNSGP +GTVSNVAPW+ITVGAS++DR F ++V L + + FK
Sbjct: 317 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 376

Query: 245 GKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNAR 304
           G SLS K LP +K+Y LI A DA   N +   A+LC+KGSLDPKKVKGKI+VCLRGDNAR
Sbjct: 377 GTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNAR 436

Query: 305 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAY 364
           ++KG   A AGA GM+L NDK +G+   +DAH+LPAS I Y DGE+++ Y+ STK P  Y
Sbjct: 437 VDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGY 496

Query: 365 MTHMTE-LGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDK 424
           +   T  L  KPAP MASFSSRGPNTI P ILKPDI APGVNIIAAF+EAT  T L  D 
Sbjct: 497 IKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDN 556

Query: 425 RMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEK 484
           R   F   SGTSMSCPHISG+VGLLKTL+P WSPAAIRSAIMTT+ T  N   P++    
Sbjct: 557 RRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESF 616

Query: 485 EKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 544
           +KANP +YG+GHVQPNKAA+PGLVYDL+  DYL+FLCA GYN   ++LF  D  + C + 
Sbjct: 617 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 676

Query: 545 FKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKT 604
             ++D NYPSI++ NL    + + R++KNVG P TY A+   P GV V V P  L F KT
Sbjct: 677 ANLLDFNYPSITVPNLTGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 736

Query: 605 GEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
           GE K F++ LR +    +  +VFG+L W+D  H V SPI V L
Sbjct: 737 GEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of CmaCh16G009990 vs. TAIR10
Match: AT2G04160.1 (AT2G04160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 667.5 bits (1721), Expect = 8.1e-192
Identity = 339/582 (58.25%), Postives = 422/582 (72.51%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARYFNKGYA+ VG LNSS+++ RD DGHG+HTLSTA G+FV  VSIFG G GTAK
Sbjct: 191 RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 125 GGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLA 184
           GGSP+ARVAAY+VCWP V    C+ AD+LA F+AAI DG DV+S+SLGG    F  D +A
Sbjct: 251 GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 185 IMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKG 244
           I S+H  K  I VVCSAGNSGP   TVSNVAPW ITVGAST+DR F S + LG+ +H+KG
Sbjct: 311 IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 245 KSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARI 304
           +SLSS  LP  K YP++ +++AK+ N S   A LC+ GSLDP K KGKI+VCLRG N R+
Sbjct: 371 QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 305 EKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYM 364
           EKG  VA  G +GM+L N    G+   AD H+LPA+ ++  D  +V +YI  TK P+A++
Sbjct: 431 EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 365 T-HMTELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKR 424
           T   T+LG+KPAP+MASFSS+GP+ + P ILKPDI APGV++IAA++ A S T   +D R
Sbjct: 491 TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 425 MVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKE 484
            + F  +SGTSMSCPHISGI GLLKT YP WSPAAIRSAIMTTA    +   PI ++   
Sbjct: 551 RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 485 KANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKSF 544
           KA P ++GAGHVQPN A NPGLVYDL I+DYLNFLC+ GYN +Q+ +F+ +     S   
Sbjct: 611 KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

Query: 545 KVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKTG 604
            +++LNYPSI++ NL S  V + R VKNVG P  Y  KV +P GV V V P++L FTK G
Sbjct: 671 SLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVG 730

Query: 605 EEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
           E+K FKV+L + + N  + +VFG+LVWSD KHRV SPI V L
Sbjct: 731 EQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CmaCh16G009990 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 522.7 bits (1345), Expect = 3.2e-148
Identity = 297/634 (46.85%), Postives = 402/634 (63.41%), Query Frame = 1

Query: 18  LLQTSTLAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGY 77
           +L T      KSY         G  PS+        T++T   S+  RKLIGAR+F +GY
Sbjct: 137 VLDTGVWPESKSY----SDEGFGPIPSSWKGGCEAGTNFTA--SLCNRKLIGARFFARGY 196

Query: 78  ASNVGPLNSSYETA--RDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAY 137
            S +GP++ S E+   RDDDGHGTHT STA G+ V+  S+ G   GTA+G +P+ARVA Y
Sbjct: 197 ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVY 256

Query: 138 RVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDI 197
           +VCW     GGCF +DILA  + AI+D V+VLS+SLGGG  ++  D +AI ++  ++  I
Sbjct: 257 KVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGI 316

Query: 198 TVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKGKSLSSKLLPTK 257
            V CSAGN+GP + ++SNVAPW+ TVGA T+DR F +   LG+ ++F G SL        
Sbjct: 317 LVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD 376

Query: 258 KLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGA 317
           KL P I A +A SN  + N   LC  G+L P+KVKGKIV+C RG NAR++KG VV  AG 
Sbjct: 377 KLLPFIYAGNA-SNATNGN---LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGG 436

Query: 318 VGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYMTHM-TELGIKP 377
           VGMILAN   NG+   ADAHLLPA+ +    G+ +  Y+ +   P A ++ + T +G+KP
Sbjct: 437 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP 496

Query: 378 APIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKRMVQFTELSGTS 437
           +P++A+FSSRGPN+I P+ILKPD+IAPGVNI+AA++ A   TGL  D R V+F  +SGTS
Sbjct: 497 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTS 556

Query: 438 MSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKEK-ANPLAYGAG 497
           MSCPH+SG+  LLK+++P+WSPAAIRSA+MTTA     D  P+L     K + P  +GAG
Sbjct: 557 MSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAG 616

Query: 498 HVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVC--SKSFKVIDLNYP 557
           HV P  A NPGL+YDL+ +DYL FLCA  Y   Q++  +   ++ C  SKS+ V DLNYP
Sbjct: 617 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYP 676

Query: 558 SISIYNLKSEVVNIKRRVKNVGSPGTYVAKVES-PPGVSVLVAPSTLKFTKTGEEKNFKV 617
           S ++           R V +VG  GTY  KV S   GV + V P+ L F +  E+K++ V
Sbjct: 677 SFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 736

Query: 618 VLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVT 645
                 S  +  + FG + WSDGKH V SP+ ++
Sbjct: 737 TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmaCh16G009990 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 476.1 bits (1224), Expect = 3.5e-134
Identity = 270/586 (46.08%), Postives = 368/586 (62.80%), Query Frame = 1

Query: 65  RKLIGARYFNKGYA-SNVGPLNSSYETA--RDDDGHGTHTLSTAGGNFVQRVSIFGNGYG 124
           +KLIGAR F+KG+  ++ G  +S  E+   RD DGHGTHT +TA G+ V+  S  G   G
Sbjct: 173 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 232

Query: 125 TAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSED 184
           TA+G + +ARVA Y+VCW    + GCF +DILA  + AI DGVDVLS+SLGGG+  +  D
Sbjct: 233 TARGMATRARVATYKVCW----STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRD 292

Query: 185 LLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRH 244
            +AI ++  ++  + V CSAGNSGP   +V+NVAPW++TVGA T+DR F ++  LG+ + 
Sbjct: 293 TIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKR 352

Query: 245 FKGKSLSSKL-LPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGD 304
             G SL S + + TK L  +      K N+ S N   LC  GSLD   V+GKIVVC RG 
Sbjct: 353 LTGVSLYSGVGMGTKPLELVYN----KGNSSSSN---LCLPGSLDSSIVRGKIVVCDRGV 412

Query: 305 NARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTP 364
           NAR+EKG VV  AG +GMI+AN   +G+   AD+HLLPA  +    G+ + +Y++S   P
Sbjct: 413 NARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKP 472

Query: 365 MAYMTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLP 424
            A +    T L +KP+P++A+FSSRGPNT+ P ILKPD+I PGVNI+A +S+A   TGL 
Sbjct: 473 TALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLD 532

Query: 425 YDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPIL- 484
            D R  QF  +SGTSMSCPHISG+ GLLK  +P+WSP+AI+SA+MTTA    N   P+  
Sbjct: 533 KDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHD 592

Query: 485 SSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFV 544
           +++   +NP A+G+GHV P KA +PGLVYD+S ++Y+ FLC+  Y    +       S  
Sbjct: 593 AADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVN 652

Query: 545 CSKSFK-VIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGT-YVAKVESPPGVSVLVAPST 604
           CSK F     LNYPS S+      VV   R V NVG+  + Y   V   P V + V PS 
Sbjct: 653 CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 712

Query: 605 LKFTKTGEEKNFKVV-LRRVQSNQTEEHVFGKLVWSDGKHRVSSPI 642
           L F   GE+K + V  + +   + T +  FG + WS+ +H V SP+
Sbjct: 713 LSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPV 747

BLAST of CmaCh16G009990 vs. TAIR10
Match: AT5G51750.1 (AT5G51750.1 subtilase 1.3)

HSP 1 Score: 469.9 bits (1208), Expect = 2.5e-132
Identity = 263/618 (42.56%), Postives = 381/618 (61.65%), Query Frame = 1

Query: 40  GLNPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGYASNVGPLNSS--YETARDDDGH 99
           G++P     + A +T    L+    RK++GAR F +GY +  G ++    Y++ RD DGH
Sbjct: 168 GMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGH 227

Query: 100 GTHTLSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFE 159
           GTHT +T  G+ V+  ++FG  YGTA+G + KARVAAY+VCW     GGCF +DIL+  +
Sbjct: 228 GTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWV----GGCFSSDILSAVD 287

Query: 160 AAISDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPW 219
            A++DGV VLSISLGGG   +S D L+I ++  ++  + V CSAGN GP   +++NV+PW
Sbjct: 288 QAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPW 347

Query: 220 MITVGASTVDRLFTSYVALGDKRHFKGKSL--SSKLLPTKKLYPLIRALDAKSNNVSDNY 279
           + TVGAST+DR F + V +G  R FKG SL     +LP  K YPL+       N  S + 
Sbjct: 348 ITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYL---GRNASSPDP 407

Query: 280 AILCRKGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAH 339
              C  G+LD + V GKIV+C RG   R++KG VV +AG +GM+L N   NG+   AD+H
Sbjct: 408 TSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSH 467

Query: 340 LLPASHISYTDGESVYQYIQSTKTPMAYMTHM-TELGIKPAPIMASFSSRGPNTIEPSIL 399
           +LPA  +   +G+ + QY  ++K   A +  + T +GIKP+P++A+FSSRGPN +   IL
Sbjct: 468 MLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEIL 527

Query: 400 KPDIIAPGVNIIAAFSEATSITGLPYDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKW 459
           KPD++APGVNI+AA++   + + L  D R V+F  LSGTSMSCPH+SG+  L+K+ +P W
Sbjct: 528 KPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDW 587

Query: 460 SPAAIRSAIMTTAGTEANDLNPIL-SSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQD 519
           SPAAI+SA+MTTA    N   P+  +S    ++P  +GAGH+ P +A +PGLVYD+  Q+
Sbjct: 588 SPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQE 647

Query: 520 YLNFLCARGYNKAQMKLFTNDTSFVCSKSF--KVIDLNYPSISIY---NLKSEVVNIKRR 579
           Y  FLC +  + +Q+K+FT  ++  C  +      +LNYP+IS     N   + + ++R 
Sbjct: 648 YFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRT 707

Query: 580 VKNVGSP-GTYVAKVESPPGVSVLVAPSTLKFTKTGEEKNFKVVLR-RVQSNQTEEHVFG 639
           V NVG    +Y   V    G SV V P TL FT   ++ ++ V  R R +  + E   FG
Sbjct: 708 VTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPE---FG 767

Query: 640 KLVWSDGKHRVSSPIFVT 645
            LVW    H+V SP+ +T
Sbjct: 768 GLVWKSTTHKVRSPVIIT 775

BLAST of CmaCh16G009990 vs. NCBI nr
Match: gi|778675733|ref|XP_011650462.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 838.2 bits (2164), Expect = 9.8e-240
Identity = 423/585 (72.31%), Postives = 494/585 (84.44%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARY+NKGYA+ VGPLNSSYE+ARD +GHGTHTLSTAGG+FV   ++FG G GTAK
Sbjct: 133 RKLIGARYYNKGYAAIVGPLNSSYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGTAK 192

Query: 125 GGSPKARVAAYRVCWPEVAN-GGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 184
           GGSPKA VAAY+VCWP+V   G CF ADILAGFEAAI DGVDVLS+SLGG   +F++D +
Sbjct: 193 GGSPKALVAAYKVCWPQVLFFGECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFAKDSI 252

Query: 185 AIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFK 244
           +I S+H V+N I VVCSAGNSGP  G+VSNVAPW+ITVGAST DRL+TSYVA+GDKRHFK
Sbjct: 253 SIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHFK 312

Query: 245 GKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNAR 304
           G S+S K LP +K YPLI +LDAK+ NV+DN A+LC +GSLDPKKV GKI++CLRGDNAR
Sbjct: 313 GASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRGDNAR 372

Query: 305 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAY 364
           + KGYV A+AGAVGMILAN +ENGD   ADAHLLPASHI+Y+DG+ VYQYI STK PMAY
Sbjct: 373 VAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKIPMAY 432

Query: 365 MTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDK 424
           MTH+ TE GIKPAP+MASFSSRGPNT++PSILKPDI APG NI+AA+S   S TG  +DK
Sbjct: 433 MTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGTDFDK 492

Query: 425 RMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEK 484
           R V F   SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIMTTAGT+ANDL PILS+ +
Sbjct: 493 RRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILSTNQ 552

Query: 485 EKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 544
           EKAN  AYGAGHV PN+AA+PGLVYDLS +DYLN+LCA+GYN AQ+K F+NDTSFVCSKS
Sbjct: 553 EKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFVCSKS 612

Query: 545 FKVIDLNYPSISIYNLKSEV-VNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTK 604
           FK+ DLNYPSISI  L+ +V V IKR++KNVGSPGTYV +V+ P GVSV V P++LKFT 
Sbjct: 613 FKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSLKFTG 672

Query: 605 TGEEKNFKVVLRRVQSNQT-EEHVFGKLVWSDGKHRVSSPIFVTL 646
             EEK+F+VVL+  + N +  +++FGKL WSDGKHRV SPI V L
Sbjct: 673 IDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDGKHRVRSPIVVRL 717

BLAST of CmaCh16G009990 vs. NCBI nr
Match: gi|700200865|gb|KGN55998.1| (hypothetical protein Csa_3G045120 [Cucumis sativus])

HSP 1 Score: 838.2 bits (2164), Expect = 9.8e-240
Identity = 423/585 (72.31%), Postives = 494/585 (84.44%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARY+NKGYA+ VGPLNSSYE+ARD +GHGTHTLSTAGG+FV   ++FG G GTAK
Sbjct: 174 RKLIGARYYNKGYAAIVGPLNSSYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGTAK 233

Query: 125 GGSPKARVAAYRVCWPEVAN-GGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 184
           GGSPKA VAAY+VCWP+V   G CF ADILAGFEAAI DGVDVLS+SLGG   +F++D +
Sbjct: 234 GGSPKALVAAYKVCWPQVLFFGECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFAKDSI 293

Query: 185 AIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFK 244
           +I S+H V+N I VVCSAGNSGP  G+VSNVAPW+ITVGAST DRL+TSYVA+GDKRHFK
Sbjct: 294 SIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHFK 353

Query: 245 GKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNAR 304
           G S+S K LP +K YPLI +LDAK+ NV+DN A+LC +GSLDPKKV GKI++CLRGDNAR
Sbjct: 354 GASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRGDNAR 413

Query: 305 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAY 364
           + KGYV A+AGAVGMILAN +ENGD   ADAHLLPASHI+Y+DG+ VYQYI STK PMAY
Sbjct: 414 VAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKIPMAY 473

Query: 365 MTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDK 424
           MTH+ TE GIKPAP+MASFSSRGPNT++PSILKPDI APG NI+AA+S   S TG  +DK
Sbjct: 474 MTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGTDFDK 533

Query: 425 RMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEK 484
           R V F   SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIMTTAGT+ANDL PILS+ +
Sbjct: 534 RRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILSTNQ 593

Query: 485 EKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 544
           EKAN  AYGAGHV PN+AA+PGLVYDLS +DYLN+LCA+GYN AQ+K F+NDTSFVCSKS
Sbjct: 594 EKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFVCSKS 653

Query: 545 FKVIDLNYPSISIYNLKSEV-VNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTK 604
           FK+ DLNYPSISI  L+ +V V IKR++KNVGSPGTYV +V+ P GVSV V P++LKFT 
Sbjct: 654 FKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSLKFTG 713

Query: 605 TGEEKNFKVVLRRVQSNQT-EEHVFGKLVWSDGKHRVSSPIFVTL 646
             EEK+F+VVL+  + N +  +++FGKL WSDGKHRV SPI V L
Sbjct: 714 IDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDGKHRVRSPIVVRL 758

BLAST of CmaCh16G009990 vs. NCBI nr
Match: gi|659096606|ref|XP_008449186.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103491136 [Cucumis melo])

HSP 1 Score: 827.4 bits (2136), Expect = 1.7e-236
Identity = 414/583 (71.01%), Postives = 483/583 (82.85%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARYFNKGYA+  G LN+SYETARD++GHGTHTLSTAGGNF+   ++FGNG GTAK
Sbjct: 189 RKLIGARYFNKGYAAYAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTAK 248

Query: 125 GGSPKARVAAYRVCWPEV-ANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 184
           GGSPKA VAAY+VCWP+V + GGCF ADILA  EAAISDGVD+LS+SLGGGA +FSED+ 
Sbjct: 249 GGSPKALVAAYKVCWPQVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDVT 308

Query: 185 AIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFK 244
           AI ++H V+  I VVCSAGNSGP  GT+ N APW++TVGAST++R FTSYVALG+K+H K
Sbjct: 309 AIGAFHAVQQGIIVVCSAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHIK 368

Query: 245 GKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNAR 304
           G SLS K+LP +K YPLI A DAK+NNVS + A LC+ GSLDPKKVKGKI++CLRG+NAR
Sbjct: 369 GASLSDKILPEQKFYPLINAADAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGENAR 428

Query: 305 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAY 364
           ++KGY  AQAGAVGMILAN ++NGD   ADAHLLP SH+SYTDG+S+YQYI  TKTPMAY
Sbjct: 429 VDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMAY 488

Query: 365 MTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDK 424
           MTH+ TELGIKPAP+MASFSSRGPNTIE SILKPDI APGVNI+AA+SE  S +G  +D 
Sbjct: 489 MTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFDN 548

Query: 425 RMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEK 484
           R + F  +SGTSMSCPHISGIVGLLKTLYP WSPAAI+SAIMTTA T ANDL+PIL++  
Sbjct: 549 RRIPFNIVSGTSMSCPHISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILNTVN 608

Query: 485 EKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 544
            KANP AYGAGHVQPN+A NPGLVYDL+  DY+NFLCA+GYNK+Q+  F + TSFVCSKS
Sbjct: 609 LKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKF-SATSFVCSKS 668

Query: 545 FKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKT 604
           FK+ D NYPSISI ++KS VV I RRVKNVG P TYVA+V+ P GVSV V P TLKFT  
Sbjct: 669 FKLTDFNYPSISIPDMKSGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGI 728

Query: 605 GEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
            EEK+FKVV+  V +N+   +VFG L+W DGKH V SPI V L
Sbjct: 729 DEEKSFKVVIGSVANNKHRGYVFGSLIWEDGKHHVRSPIVVNL 770

BLAST of CmaCh16G009990 vs. NCBI nr
Match: gi|700200864|gb|KGN55997.1| (hypothetical protein Csa_3G045110 [Cucumis sativus])

HSP 1 Score: 817.8 bits (2111), Expect = 1.4e-233
Identity = 410/606 (67.66%), Postives = 494/606 (81.52%), Query Frame = 1

Query: 42  NPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHT 101
           NPS +   ++   +  L++    RKLIGA+YFNKGYA+N G LN+SYETARD++GHGTHT
Sbjct: 29  NPSLLSFSISF--YIVLIKFQKYRKLIGAKYFNKGYAANAGSLNASYETARDNEGHGTHT 88

Query: 102 LSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAYRVCWPEV-ANGGCFMADILAGFEAAI 161
           LSTAGGNF+   ++FGNG GTAKGGSPKA VAAY+VCWP+V + GGC+ ADILA  EAAI
Sbjct: 89  LSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWPQVDSGGGCYDADILAAMEAAI 148

Query: 162 SDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMIT 221
           SDGVDVLS+SLGGG+ +FS+D+ AI ++H V+  I VVCSAGNSGP  GTV NVAPW+IT
Sbjct: 149 SDGVDVLSLSLGGGSKDFSDDVTAIGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIIT 208

Query: 222 VGASTVDRLFTSYVALGDKRHFKGKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCR 281
           VGAST++R FTS+V+LG+K+H KG SLS K+LP +K YPLI A+DAK+N VS + A LC 
Sbjct: 209 VGASTINRDFTSFVSLGNKKHIKGASLSDKILPEQKFYPLIDAVDAKANKVSSDIAQLCL 268

Query: 282 KGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPAS 341
            GSLDP+KVKGKI++CLRG+NAR +KGY   +AGAVGMILAN +ENGD   ADAHLLP S
Sbjct: 269 VGSLDPEKVKGKIIICLRGENARADKGYAAVKAGAVGMILANAEENGDEIIADAHLLPVS 328

Query: 342 HISYTDGESVYQYIQSTKTPMAYMTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDII 401
           H+SYTDG+S+YQYI STKTPMAYMTH+ TELGIKPAP+MASFSSRGPNT+E +ILKPDI 
Sbjct: 329 HVSYTDGQSIYQYINSTKTPMAYMTHVRTELGIKPAPVMASFSSRGPNTVEETILKPDIT 388

Query: 402 APGVNIIAAFSEATSITGLPYDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAI 461
           APGVNI+AA+SE  S +G  +DKR + F  +SGTSMSCPHISGIVGLLKTLYP WSPAAI
Sbjct: 389 APGVNILAAYSEDASPSGSFFDKRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAI 448

Query: 462 RSAIMTTAGTEANDLNPILSSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLC 521
           +SAIMTTA + AND++PI +    KAN  AYGAGHVQPN+A NPGLVYDL+ +DY+NFLC
Sbjct: 449 KSAIMTTAESRANDMHPIQNGGNLKANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLC 508

Query: 522 ARGYNKAQMKLFTNDTSFVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYV 581
           A+GYNK+Q+  F +  SFVCSKSFK+ D NYPSISI ++KS VV IKRRVKNVG P TYV
Sbjct: 509 AQGYNKSQISKF-SAMSFVCSKSFKLTDFNYPSISIPDMKSGVVTIKRRVKNVGKPSTYV 568

Query: 582 AKVESPPGVSVLVAPSTLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSS 641
           A+V+ P GVSV V P TLKFT+  EEK+FKVV+  V +N+ + +VFG L+W DGKH V S
Sbjct: 569 ARVKVPHGVSVSVEPRTLKFTRIDEEKSFKVVIGSVANNKHKGYVFGSLIWEDGKHHVRS 628

Query: 642 PIFVTL 646
           PI V L
Sbjct: 629 PIVVNL 631

BLAST of CmaCh16G009990 vs. NCBI nr
Match: gi|778688353|ref|XP_011652727.1| (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus])

HSP 1 Score: 755.4 bits (1949), Expect = 8.4e-215
Identity = 385/582 (66.15%), Postives = 459/582 (78.87%), Query Frame = 1

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGA+YFNKGYA+N G LN+SYETARD++GHGTHTLSTAGGNF+   +   NG     
Sbjct: 187 RKLIGAKYFNKGYAANAGSLNASYETARDNEGHGTHTLSTAGGNFISGANGPLNG----- 246

Query: 125 GGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLA 184
                               GGC+ ADILA  EAAISDGVDVLS+SLGGG+ +FS+D+ A
Sbjct: 247 --------------------GGCYDADILAAMEAAISDGVDVLSLSLGGGSKDFSDDVTA 306

Query: 185 IMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKG 244
           I ++H V+  I VVCSAGNSGP  GTV NVAPW+ITVGAST++R FTS+V+LG+K+H KG
Sbjct: 307 IGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIITVGASTINRDFTSFVSLGNKKHIKG 366

Query: 245 KSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARI 304
            SLS K+LP +K YPLI A+DAK+N VS + A LC  GSLDP+KVKGKI++CLRG+NAR 
Sbjct: 367 ASLSDKILPEQKFYPLIDAVDAKANKVSSDIAQLCLVGSLDPEKVKGKIIICLRGENARA 426

Query: 305 EKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYM 364
           +KGY   +AGAVGMILAN +ENGD   ADAHLLP SH+SYTDG+S+YQYI STKTPMAYM
Sbjct: 427 DKGYAAVKAGAVGMILANAEENGDEIIADAHLLPVSHVSYTDGQSIYQYINSTKTPMAYM 486

Query: 365 THM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKR 424
           TH+ TELGIKPAP+MASFSSRGPNT+E +ILKPDI APGVNI+AA+SE  S +G  +DKR
Sbjct: 487 THVRTELGIKPAPVMASFSSRGPNTVEETILKPDITAPGVNILAAYSEDASPSGSFFDKR 546

Query: 425 MVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKE 484
            + F  +SGTSMSCPHISGIVGLLKTLYP WSPAAI+SAIMTTA + AND++PI +    
Sbjct: 547 RIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAESRANDMHPIQNGGNL 606

Query: 485 KANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKSF 544
           KAN  AYGAGHVQPN+A NPGLVYDL+ +DY+NFLCA+GYNK+Q+  F +  SFVCSKSF
Sbjct: 607 KANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLCAQGYNKSQISKF-SAMSFVCSKSF 666

Query: 545 KVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKTG 604
           K+ D NYPSISI ++KS VV IKRRVKNVG P TYVA+V+ P GVSV V P TLKFT+  
Sbjct: 667 KLTDFNYPSISIPDMKSGVVTIKRRVKNVGKPSTYVARVKVPHGVSVSVEPRTLKFTRID 726

Query: 605 EEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
           EEK+FKVV+  V +N+ + +VFG L+W DGKH V SPI V L
Sbjct: 727 EEKSFKVVIGSVANNKHKGYVFGSLIWEDGKHHVRSPIVVNL 742

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT54_ARATH5.3e-19360.55Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1[more]
AIR3_ARATH1.4e-19058.25Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1[more]
SBT17_ARATH5.7e-14746.85Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1[more]
SBT1_SOYBN7.0e-14548.26Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3[more]
SBT18_ARATH6.1e-13346.08Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2R9_CUCSA6.8e-24072.31Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1[more]
A0A0A0L613_CUCSA9.6e-23467.66Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045110 PE=4 SV=1[more]
B9N5M9_POPTR8.2e-20964.26Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16870g PE=4 SV=1[more]
F6I593_VITVI4.9e-20662.59Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g01420 PE=4 SV=... [more]
W9QR32_9ROSA6.5e-20663.05Subtilisin-like protease OS=Morus notabilis GN=L484_023738 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59810.13.0e-19460.55 Subtilase family protein[more]
AT2G04160.18.1e-19258.25 Subtilisin-like serine endopeptidase family protein[more]
AT5G67360.13.2e-14846.85 Subtilase family protein[more]
AT2G05920.13.5e-13446.08 Subtilase family protein[more]
AT5G51750.12.5e-13242.56 subtilase 1.3[more]
Match NameE-valueIdentityDescription
gi|778675733|ref|XP_011650462.1|9.8e-24072.31PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus][more]
gi|700200865|gb|KGN55998.1|9.8e-24072.31hypothetical protein Csa_3G045120 [Cucumis sativus][more]
gi|659096606|ref|XP_008449186.1|1.7e-23671.01PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103491136 [Cucumis me... [more]
gi|700200864|gb|KGN55997.1|1.4e-23367.66hypothetical protein Csa_3G045110 [Cucumis sativus][more]
gi|778688353|ref|XP_011652727.1|8.4e-21566.15PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR003137PA_domain
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G009990.1CmaCh16G009990.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 64..236
score: 1.7E-63coord: 389..501
score: 1.7
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 85..493
score: 2.5
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 378..505
score: 3.01E-66coord: 64..265
score: 3.01
IPR003137PA domainPFAMPF02225PAcoord: 279..349
score: 9.5
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 431..447
score: 1.2E-7coord: 93..106
score: 1.
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 65..647
score:
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 432..442
scor
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 276..388
score: 1.
NoneNo IPR availablePANTHERPTHR10795:SF3SUBFAMILY NOT NAMEDcoord: 65..647
score:
NoneNo IPR availableunknownSSF52025PA domaincoord: 286..360
score: 2.6

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh16G009990CmoCh16G010430Cucurbita moschata (Rifu)cmacmoB323
CmaCh16G009990Cp4.1LG14g08240Cucurbita pepo (Zucchini)cmacpeB345
The following gene(s) are paralogous to this gene:

None