CmaCh16G009860 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G009860
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionSubtilisin-like serine protease
LocationCma_Chr16 : 7591915 .. 7592130 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATAATGTCCTTCCATGCCGTGAAGAATGACATCACTGTTGTTTGTTCGGCTGGAAATTCAGGACCAACTGGAAGCATGGTCTCAAATGTCGCACCATGGATGATAAATGTGGGAGTTAGCACAATTGACAGGCTTTTTACCACTTATGTGGTGTTGGGAGACAAGAGGCGCTTGAAGATACTCTATCCGACACTTATTAGTACTATATAG

mRNA sequence

ATGGCTATAATGTCCTTCCATGCCGTGAAGAATGACATCACTGTTGTTTGTTCGGCTGGAAATTCAGGACCAACTGGAAGCATGGTCTCAAATGTCGCACCATGGATGATAAATGTGGGAGTTAGCACAATTGACAGGCTTTTTACCACTTATGTGGTGTTGGGAGACAAGAGGCGCTTGAAGATACTCTATCCGACACTTATTAGTACTATATAG

Coding sequence (CDS)

ATGGCTATAATGTCCTTCCATGCCGTGAAGAATGACATCACTGTTGTTTGTTCGGCTGGAAATTCAGGACCAACTGGAAGCATGGTCTCAAATGTCGCACCATGGATGATAAATGTGGGAGTTAGCACAATTGACAGGCTTTTTACCACTTATGTGGTGTTGGGAGACAAGAGGCGCTTGAAGATACTCTATCCGACACTTATTAGTACTATATAG

Protein sequence

MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRLKILYPTLISTI
BLAST of CmaCh16G009860 vs. Swiss-Prot
Match: AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.6e-13
Identity = 38/61 (62.30%), Postives = 44/61 (72.13%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHA K  I VVCSAGNSGP  S VSNVAPW I VG ST+DR F + +VLG+ +  
Sbjct: 309 VAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHY 368

Query: 61  K 62
           K
Sbjct: 369 K 369

BLAST of CmaCh16G009860 vs. Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.5e-13
Identity = 37/61 (60.66%), Postives = 45/61 (73.77%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHAVKN +TVVCSAGNSGP    VSNVAPW+I VG S++DR F  +V L + +  
Sbjct: 316 IAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSF 375

Query: 61  K 62
           K
Sbjct: 376 K 376

BLAST of CmaCh16G009860 vs. Swiss-Prot
Match: SBT51_ARATH (Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana GN=SBT5.1 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.1e-11
Identity = 36/61 (59.02%), Postives = 44/61 (72.13%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVL-GDKRR 60
           ++I SFHAV+  ITVVCS GNSGP+   V N APWMI V  STIDR F + ++L GD+ R
Sbjct: 297 LSIGSFHAVERGITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDENR 356

BLAST of CmaCh16G009860 vs. Swiss-Prot
Match: CRSP_ARATH (CO(2)-response secreted protease OS=Arabidopsis thaliana GN=CRSP PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 7.2e-11
Identity = 32/61 (52.46%), Postives = 43/61 (70.49%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI +FHAV+  I V+CSAGN GP G  V+N APW++ V  +TIDR F + VVLG  + +
Sbjct: 295 IAIGAFHAVEQGILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVI 354

Query: 61  K 62
           K
Sbjct: 355 K 355

BLAST of CmaCh16G009860 vs. Swiss-Prot
Match: SBT38_ARATH (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 8.0e-10
Identity = 29/58 (50.00%), Postives = 38/58 (65.52%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKR 59
           +A  +FHAV   ITVVCS GNSGP    V N APW++ V  +T+DR F T + LG+ +
Sbjct: 310 IATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNK 367

BLAST of CmaCh16G009860 vs. TrEMBL
Match: A0A0A0L2R9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.4e-15
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           ++I SFHAV+N I VVCSAGNSGPT   VSNVAPW+I VG ST DRL+T+YV +GDKR  
Sbjct: 293 ISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHF 352

Query: 61  K 62
           K
Sbjct: 353 K 353

BLAST of CmaCh16G009860 vs. TrEMBL
Match: A0A059DDV3_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A00959 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 4.9e-14
Identity = 39/61 (63.93%), Postives = 49/61 (80.33%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHA++N I VVCSAGNSGP+   VSN++PW+I V  ST+DR F+ YVV+GDKRR 
Sbjct: 306 IAIGSFHAMRNGIVVVCSAGNSGPSDGTVSNISPWLITVAASTMDRDFSNYVVIGDKRRF 365

Query: 61  K 62
           K
Sbjct: 366 K 366

BLAST of CmaCh16G009860 vs. TrEMBL
Match: A0A059DDD2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A00959 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 4.9e-14
Identity = 39/61 (63.93%), Postives = 49/61 (80.33%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHA++N I VVCSAGNSGP+   VSN++PW+I V  ST+DR F+ YVV+GDKRR 
Sbjct: 306 IAIGSFHAMRNGIVVVCSAGNSGPSDGTVSNISPWLITVAASTMDRDFSNYVVIGDKRRF 365

Query: 61  K 62
           K
Sbjct: 366 K 366

BLAST of CmaCh16G009860 vs. TrEMBL
Match: A0A059BW60_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03663 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 6.3e-14
Identity = 43/61 (70.49%), Postives = 46/61 (75.41%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHA +N I VV SAGNSGP    V NVAPWMI V  STIDR FTTYV LGDK+RL
Sbjct: 290 IAIGSFHAARNGIVVVSSAGNSGPQPGTVCNVAPWMITVAASTIDREFTTYVALGDKQRL 349

Query: 61  K 62
           K
Sbjct: 350 K 350

BLAST of CmaCh16G009860 vs. TrEMBL
Match: A0A022R471_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a001745mg PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.4e-13
Identity = 40/61 (65.57%), Postives = 48/61 (78.69%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHAV++ + VVCSAGNSGP    VSNVAPW I VG ST+DR F +YVVLG+K RL
Sbjct: 302 VAIGSFHAVRHGVVVVCSAGNSGPGAGTVSNVAPWQITVGASTMDRQFPSYVVLGNKMRL 361

Query: 61  K 62
           +
Sbjct: 362 R 362

BLAST of CmaCh16G009860 vs. TAIR10
Match: AT2G04160.1 (AT2G04160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 75.5 bits (184), Expect = 1.5e-14
Identity = 38/61 (62.30%), Postives = 44/61 (72.13%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHA K  I VVCSAGNSGP  S VSNVAPW I VG ST+DR F + +VLG+ +  
Sbjct: 309 VAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHY 368

Query: 61  K 62
           K
Sbjct: 369 K 369

BLAST of CmaCh16G009860 vs. TAIR10
Match: AT5G59810.1 (AT5G59810.1 Subtilase family protein)

HSP 1 Score: 75.1 bits (183), Expect = 1.9e-14
Identity = 37/61 (60.66%), Postives = 45/61 (73.77%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHAVKN +TVVCSAGNSGP    VSNVAPW+I VG S++DR F  +V L + +  
Sbjct: 316 IAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSF 375

Query: 61  K 62
           K
Sbjct: 376 K 376

BLAST of CmaCh16G009860 vs. TAIR10
Match: AT1G20150.1 (AT1G20150.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 70.1 bits (170), Expect = 6.3e-13
Identity = 36/61 (59.02%), Postives = 44/61 (72.13%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVL-GDKRR 60
           ++I SFHAV+  ITVVCS GNSGP+   V N APWMI V  STIDR F + ++L GD+ R
Sbjct: 297 LSIGSFHAVERGITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDENR 356

BLAST of CmaCh16G009860 vs. TAIR10
Match: AT1G20160.1 (AT1G20160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 67.4 bits (163), Expect = 4.1e-12
Identity = 32/61 (52.46%), Postives = 43/61 (70.49%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI +FHAV+  I V+CSAGN GP G  V+N APW++ V  +TIDR F + VVLG  + +
Sbjct: 295 IAIGAFHAVEQGILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVI 354

Query: 61  K 62
           K
Sbjct: 355 K 355

BLAST of CmaCh16G009860 vs. TAIR10
Match: AT4G10540.1 (AT4G10540.1 Subtilase family protein)

HSP 1 Score: 63.9 bits (154), Expect = 4.5e-11
Identity = 29/58 (50.00%), Postives = 38/58 (65.52%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKR 59
           +A  +FHAV   ITVVCS GNSGP    V N APW++ V  +T+DR F T + LG+ +
Sbjct: 310 IATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNK 367

BLAST of CmaCh16G009860 vs. NCBI nr
Match: gi|659096606|ref|XP_008449186.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103491136 [Cucumis melo])

HSP 1 Score: 89.4 bits (220), Expect = 2.8e-15
Identity = 43/61 (70.49%), Postives = 48/61 (78.69%), Query Frame = 1

Query: 1    MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
            +AI SFHAV+N ITVVCSAGNSGP    VSNVAPW+I VG ST DRL+T YV +GDKR  
Sbjct: 1049 IAIGSFHAVQNGITVVCSAGNSGPAPGTVSNVAPWIITVGASTADRLYTNYVAIGDKRHF 1108

Query: 61   K 62
            K
Sbjct: 1109 K 1109

BLAST of CmaCh16G009860 vs. NCBI nr
Match: gi|778675733|ref|XP_011650462.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 88.6 bits (218), Expect = 4.8e-15
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           ++I SFHAV+N I VVCSAGNSGPT   VSNVAPW+I VG ST DRL+T+YV +GDKR  
Sbjct: 252 ISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHF 311

Query: 61  K 62
           K
Sbjct: 312 K 312

BLAST of CmaCh16G009860 vs. NCBI nr
Match: gi|700200865|gb|KGN55998.1| (hypothetical protein Csa_3G045120 [Cucumis sativus])

HSP 1 Score: 88.6 bits (218), Expect = 4.8e-15
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           ++I SFHAV+N I VVCSAGNSGPT   VSNVAPW+I VG ST DRL+T+YV +GDKR  
Sbjct: 293 ISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHF 352

Query: 61  K 62
           K
Sbjct: 353 K 353

BLAST of CmaCh16G009860 vs. NCBI nr
Match: gi|657951534|ref|XP_008353281.1| (PREDICTED: subtilisin-like protease [Malus domestica])

HSP 1 Score: 85.5 bits (210), Expect = 4.1e-14
Identity = 43/61 (70.49%), Postives = 48/61 (78.69%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHAVK  ITVV SAGNSGPT   VSNVAPWM+ VG STIDR F+ YV LG+K+ L
Sbjct: 311 IAIGSFHAVKKGITVVSSAGNSGPTPGSVSNVAPWMLTVGASTIDREFSNYVALGNKKHL 370

Query: 61  K 62
           K
Sbjct: 371 K 371

BLAST of CmaCh16G009860 vs. NCBI nr
Match: gi|658018787|ref|XP_008344750.1| (PREDICTED: subtilisin-like protease [Malus domestica])

HSP 1 Score: 85.5 bits (210), Expect = 4.1e-14
Identity = 43/61 (70.49%), Postives = 48/61 (78.69%), Query Frame = 1

Query: 1   MAIMSFHAVKNDITVVCSAGNSGPTGSMVSNVAPWMINVGVSTIDRLFTTYVVLGDKRRL 60
           +AI SFHAVK  ITVV SAGNSGPT   VSNVAPWM+ VG STIDR F+ YV LG+K+ L
Sbjct: 165 IAIGSFHAVKKGITVVSSAGNSGPTPGSVSNVAPWMLTVGASTIDREFSNYVALGNKKHL 224

Query: 61  K 62
           K
Sbjct: 225 K 225

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AIR3_ARATH2.6e-1362.30Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1[more]
SBT54_ARATH3.5e-1360.66Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1[more]
SBT51_ARATH1.1e-1159.02Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana GN=SBT5.1 PE=3 SV=1[more]
CRSP_ARATH7.2e-1152.46CO(2)-response secreted protease OS=Arabidopsis thaliana GN=CRSP PE=2 SV=1[more]
SBT38_ARATH8.0e-1050.00Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana GN=SBT3.8 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2R9_CUCSA3.4e-1568.85Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1[more]
A0A059DDV3_EUCGR4.9e-1463.93Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A00959 PE=4 SV=1[more]
A0A059DDD2_EUCGR4.9e-1463.93Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A00959 PE=4 SV=1[more]
A0A059BW60_EUCGR6.3e-1470.49Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03663 PE=4 SV=1[more]
A0A022R471_ERYGU1.4e-1365.57Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a001745mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G04160.11.5e-1462.30 Subtilisin-like serine endopeptidase family protein[more]
AT5G59810.11.9e-1460.66 Subtilase family protein[more]
AT1G20150.16.3e-1359.02 Subtilisin-like serine endopeptidase family protein[more]
AT1G20160.14.1e-1252.46 Subtilisin-like serine endopeptidase family protein[more]
AT4G10540.14.5e-1150.00 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|659096606|ref|XP_008449186.1|2.8e-1570.49PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103491136 [Cucumis me... [more]
gi|778675733|ref|XP_011650462.1|4.8e-1568.85PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus][more]
gi|700200865|gb|KGN55998.1|4.8e-1568.85hypothetical protein Csa_3G045120 [Cucumis sativus][more]
gi|657951534|ref|XP_008353281.1|4.1e-1470.49PREDICTED: subtilisin-like protease [Malus domestica][more]
gi|658018787|ref|XP_008344750.1|4.1e-1470.49PREDICTED: subtilisin-like protease [Malus domestica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR015500Peptidase_S8_subtilisin-rel
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G009860.1CmaCh16G009860.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 4..49
score: 4.
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 2..57
score: 8.25
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 1..61
score: 4.9
NoneNo IPR availablePANTHERPTHR10795:SF3SUBFAMILY NOT NAMEDcoord: 1..61
score: 4.9

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh16G009860Cp4.1LG14g08080Cucurbita pepo (Zucchini)cmacpeB345
CmaCh16G009860CsGy3G003870Cucumber (Gy14) v2cgybcmaB340
The following gene(s) are paralogous to this gene:

None