CmaCh16G009850 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G009850
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionSubtilisin-like serine protease
LocationCma_Chr16 : 7580323 .. 7584310 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACATAGGCCATTTAGGTTTGTTATTATAAAGCTGAAAAGAATGGGAGGAGTTGCTCATTCATAAGCTTCAAGAACAAAGCTATGGAGGGTTTCAATCTATCTTCATTGATTCTTCTCTTCTTTTTTTTCCCTCTGTTGCAAACATCCACCATTGCCACCCAAAAGGCAAGCCAATGATCAACCATTATTTTGATTTTTTTCTTTCTTCGATCCTATTTCTCATTTGTTCACCTCGTGCAGTCTTACATTGTTTACTTGGGATCACATTCGCACGGCTTGAATCCGTCATCAGCCGATCTCCAAGTCGCAACGGAATCTCATTACAGTTTGCTCGGGTCATTGTTAGGAAGGTGAAACTTCTAGTCTATATCATTAAATTATATTTTTCAATTTGCTTAATCTTCTTATGTTTTCAAGTATAGCAATGAGATGGCCAAGGAAGCAATCTTCTACTCCTACAACAAATATATCAATGGCTTTGCTGCCGTTCTTGACCATAAAGTTATGCAAGATCTAGCAAGTAAACCATTCGATCCAATACATAATATGAATCGAAACTCATTTTGCTGCCTAATTGGCTACATATCATGGTAGGACATCCCGCTGTCGTATCGATTCATGAGAACAAGCTGAGAAAGCTGCACACAACAAATTCATGGGAATTTCTTGAGCTAGAGAATGGTGAAGGAACTCCTCCAAATTCCATTTGGAATGCTTCAAATTTTGGTGAATCTACCATCATTGCCAACCTTGACACAGGTTAGTCCTGATTATCACTATAAGAAATAATGAATACGATAGCTTACGAAAAATACTATTATAGACTTTTTATAGCATTTTTTGTACGTCGTGACAACCCATGTTATTAAAAGTCTTGAATTTCTTAAAGCAATTTTTTATACCTATAATAGACGGTAATAGAGTATGAAATTATAGCTTATATATGTTGCTATGAAAACATTCATGTAATTAAAAGTCTAGAATTTTTATAGCGTGTTTTTTATAGCTATAACGGATGTTATAATAGAGTATGAAGCTATAACTTATTTATGTTGCTAGGAAAACATTCATGCTATTAAAAGTCTGAGATTTTTATAGTGTTTTCTTATAGTTATAACAGAATATGAAACTATAAATAAATGAAATATTTATATTATTAAAAGTCTGGAAATTTTTAAAGTATTTTTTTTATAGCTATAATAGAGTATGAAACTATAACTTATATATGTTGCTATGTTATTAAAAGTTTGAAATTTTTTAGAGCTATAATAAAGTATGAAACTATAACTTATATATGTTGTTATGAAAAGATTTATGTCTGAGATTTTTTATAACATTTTTTAATAGCTATAATGGATAGGAAACTATAACTTATATATGTTTCTATGAAAACATTACTTATACAGGTTGCTATGAAAACATTTTATAGGGTTTTATTTCTAAGTTTTTGACCATTCTAAAATTTGGATTGGTATAGGCGTTTGGCCAGAATCAAAGAGTTTCAGTGACGAAGGATATGAGGCTATTCCCTCGAGGTGGAGGGGAAGTTGCGAGGGTGGCTCTAATTTTCATTGCAACAAGAAGCTAATTGGAGCACGGTATTTCAATAAAGGTTATGTCGCCCTTGCGGGATCCCTTGATGTTAGCTTTGACACAGCAAGGGACCAAGAAGGGCATGGAACACACACTTTATCCACGGCTGGAGGCAGTTTTGTTTCGGGAGCTAACGTTTTTGGTTATGGTAATGGTACTGCAAAAGGGGGTTCGCCTAAAGCCCGTGTTGCTGCCTATAAAGTATGTTGGCTTACATTTCGTGGTGGTCAGTGTTCGGATGCAGACATCCTAGCCGCCATAGAAGCTGCTATTACTGATGGTGTTGATGTTCTCTCACTTTCACTCGGTCGAGGTTCCATGGAGTTTTTCGACGACGTAATAGCGATTGGAGCCTTCCATGCGGTTCAACAAGGGATGGTCGTCGTCTGCGCAGGTGGAAACTCAGGACCCGATCTACAGAGCGTAGAAAACGTGGCGCCTTGGCTTTTCACTGTGGCTGCTAGCACAATCAACAGACAATTTACTAGTTATGTGGCCCTTGGAAACGAGAAGAATATCTCGGTACTCATTTTAATCCTCTATTTTCTCATGTTTTTTTTGCCATCTTTAATGATCTTCAAACTATCAACCCTAGGGAGCAAGTCTTTCGGACAAAATATTGTCAGCTCAACAATTCTATCCATTGATCACTTCCGCAGATGCGAAAGCTATCAATGTCTCCGTTGAAATCGCGTAAGTATAGGTTTAAACATTATTTTGATCCATATACTATTTATATTGGTTCATTTTCATCCGTATATATTTGAAACGTCCATTTTGGTCCATATACTTTTAAAGTTGGACTAAAATGGTCACTTTTTAGGTAAAAACACTAATGCGGAAATTGTGATGCATCCTTGCAGTAAACTATGTGTGGAGGGGTCTCTTGATCCGAGAAAGGTGAAAGGGAAGATTATAGTTTGCGTTAGAGGGGACGATGCAAGAGTGAACAAGGGTTTTGTGGCTGCTAAAGCAGGTGCTGTTGGAATGATTCTAGCCAACAACGAGGAATATGGGAATGATGTTGTAGCCGATGCACACATCCTTCCTGTCTCCCACATAAGTTATGTCGATGGCGAAACCGTCTACGAATACATCAACTCCACCAAGTAATTACTTCATCTATTGAAATTGAACGACTTTTTAAGTGTTTGAAAAAGTGTTTTTAATAAATTAAGCACTTAGAAAGTCTTTCCTAACAAACTCTTAGTCACAAAACTTATCGTTATACTTTAACAACTAGAACTCCAGTGGCTTACATGACTCATGTAAGGACAGAGATGGGAATCAAACCTGCACCTGTTATGGCTTCATTCTCATCTAGAGGTCCCAGTTCAATTGAGGAGTCGATACTCAAGGTTTCCATGTCTGCTCTCCTTTTTTTTACATTGTGTTTCTACCGTAGAATATTCAATGCTCAACTCGAAATCTTAAGTGTGTATCTCACTTTTTCTTTTCTTCAGCCTGATATAACAGCACCGGGCATCAATATAATTGCAGCCTATTCAAAAGATGCATCACCAAGTGGTTCACCATTTGATAACCGTCGAATTCCATTTAATCTAGTATCTGGCACTTCCATGTCATGTCCGCATATTTCTGGTATTGTTGCCCTTCTCAAGACCATTTATCCAAAATGGAGTCCAGCAGCTATCAAATCTGCAATCATGACCACAGGTACATTCAAATTATCAAATTATTACGTAAATAGTTGGGGCTAAGATATAAAGGATTGGCTGCATTAAAGTGTTCGATATAATCAAATTTAGTGTAACCAATTAGAGCTCCATAGTGATTCGAGAAGTTATTTGTGCTCATACCCATTGTAATAACATTTCCTTCACTTCTTCAGCTGAAACTAGAGCCAATGACTTGCATCCAATACTAGATTTCACCAAGCTTGTAGCCAACCCATTGGCGTACGGTGCGGGACATGTGCAACCCAACCGAGCAGCAAATCCTGGCCTTGTTTATGACCTTACCACCAATGACTATTTAAATTTTTTATGTGCTCGAGGCTACAATAAAGCACAACTCAGTAAATTCTCCAATACGTCATTCGTTTGTTCAAAGTCATTCAAACTAACAGATTTCAACTACCCATCAATCTCGATACCCAATATGAAATCAGGACCTGTGACAATCAAAAGAACAGTTAAGAATGTGGGAAGCCCAAGTACATATGTAGCTCGGGTGGAGGTACCCCCAGGAGTTTTAGTTTCAATTAAGCCAAGTACGTTGAAGTTTACTCGAACTGATGAAGAGAAGACTTTCGAAGTTGTATTCCAAAGTGTGGCAAACAACAAGCATCGAGGCTATGTATTTGGGTCTTTGAAATGGTTAGATGGTAAGCATCATGTAAGAAGCTCAATTGTAGTGAATTTGGGATGA

mRNA sequence

ATGAACATAGGCCATTTAGTCTTACATTGTTTACTTGGGATCACATTCGCACGGCTTGAATCCGTCATCAGCCGATCTCCAAGTCGCAACGGAATCTCATTACAGTTTGCTCGGGTCATTGTTAGGAAGTATAGCAATGAGATGGCCAAGGAAGCAATCTTCTACTCCTACAACAAATATATCAATGGCTTTGCTGCCGTTCTTGACCATAAAGTTATGCAAGATCTAGCAAGACATCCCGCTGTCGTATCGATTCATGAGAACAAGCTGAGAAAGCTGCACACAACAAATTCATGGGAATTTCTTGAGCTAGAGAATGGATATGAGGCTATTCCCTCGAGGTGGAGGGGAAGTTGCGAGGGTGGCTCTAATTTTCATTGCAACAAGAAGCTAATTGGAGCACGGTATTTCAATAAAGGTTATGTCGCCCTTGCGGGATCCCTTGATGTTAGCTTTGACACAGCAAGGGACCAAGAAGGGCATGGAACACACACTTTATCCACGGCTGGAGGCAGTTTTGTTTCGGGAGCTAACGTTTTTGGTTATGGTAATGGTACTGCAAAAGGGGGTTCGCCTAAAGCCCGTGTTGCTGCCTATAAAGTATGTTGGCTTACATTTCGTGGTGGTCAGTGTTCGGATGCAGACATCCTAGCCGCCATAGAAGCTGCTATTACTGATGGTGTTGATGTTCTCTCACTTTCACTCGGTCGAGGTTCCATGGAGTTTTTCGACGACGTAATAGCGATTGGAGCCTTCCATGCGGTTCAACAAGGGATGGTCGTCGTCTGCGCAGGTGGAAACTCAGGACCCGATCTACAGAGCGTAGAAAACGTGGCGCCTTGGCTTTTCACTGTGGCTGCTAGCACAATCAACAGACAATTTACTAGTTATGTGGCCCTTGGAAACGAGAAGAATATCTCGGGGTCTCTTGATCCGAGAAAGGTGAAAGGGAAGATTATAGTTTGCGTTAGAGGGGACGATGCAAGAGTGAACAAGGGTTTTGTGGCTGCTAAAGCAGGTGCTGTTGGAATGATTCTAGCCAACAACGAGGAATATGGGAATGATGTTGTAGCCGATGCACACATCCTTCCTGTCTCCCACATAAAGATGGGAATCAAACCTGCACCTGTTATGGCTTCATTCTCATCTAGAGGTCCCAGTTCAATTGAGGAGTCGATACTCAAGCCTGATATAACAGCACCGGGCATCAATATAATTGCAGCCTATTCAAAAGATGCATCACCAAGTGGTTCACCATTTGATAACCGTCGAATTCCATTTAATCTAGTATCTGGCACTTCCATGTCATGTCCGCATATTTCTGGTATTGTTGCCCTTCTCAAGACCATTTATCCAAAATGGAGTCCAGCAGCTATCAAATCTGCAATCATGACCACAGCTGAAACTAGAGCCAATGACTTGCATCCAATACTAGATTTCACCAAGCTTGTAGCCAACCCATTGGCGTACGGTGCGGGACATGTGCAACCCAACCGAGCAGCAAATCCTGGCCTTGTTTATGACCTTACCACCAATGACTATTTAAATTTTTTATGTGCTCGAGGCTACAATAAAGCACAACTCAGTAAATTCTCCAATACGTCATTCGTTTGTTCAAAGTCATTCAAACTAACAGATTTCAACTACCCATCAATCTCGATACCCAATATGAAATCAGGACCTGTGACAATCAAAAGAACAGTTAAGAATGTGGGAAGCCCAAGTACATATGTAGCTCGGGTGGAGGTACCCCCAGGAGTTTTAGTTTCAATTAAGCCAAGTACGTTGAAGTTTACTCGAACTGATGAAGAGAAGACTTTCGAAGTTGTATTCCAAAGTGTGGCAAACAACAAGCATCGAGGCTATGTATTTGGGTCTTTGAAATGGTTAGATGGTAAGCATCATGTAAGAAGCTCAATTGTAGTGAATTTGGGATGA

Coding sequence (CDS)

ATGAACATAGGCCATTTAGTCTTACATTGTTTACTTGGGATCACATTCGCACGGCTTGAATCCGTCATCAGCCGATCTCCAAGTCGCAACGGAATCTCATTACAGTTTGCTCGGGTCATTGTTAGGAAGTATAGCAATGAGATGGCCAAGGAAGCAATCTTCTACTCCTACAACAAATATATCAATGGCTTTGCTGCCGTTCTTGACCATAAAGTTATGCAAGATCTAGCAAGACATCCCGCTGTCGTATCGATTCATGAGAACAAGCTGAGAAAGCTGCACACAACAAATTCATGGGAATTTCTTGAGCTAGAGAATGGATATGAGGCTATTCCCTCGAGGTGGAGGGGAAGTTGCGAGGGTGGCTCTAATTTTCATTGCAACAAGAAGCTAATTGGAGCACGGTATTTCAATAAAGGTTATGTCGCCCTTGCGGGATCCCTTGATGTTAGCTTTGACACAGCAAGGGACCAAGAAGGGCATGGAACACACACTTTATCCACGGCTGGAGGCAGTTTTGTTTCGGGAGCTAACGTTTTTGGTTATGGTAATGGTACTGCAAAAGGGGGTTCGCCTAAAGCCCGTGTTGCTGCCTATAAAGTATGTTGGCTTACATTTCGTGGTGGTCAGTGTTCGGATGCAGACATCCTAGCCGCCATAGAAGCTGCTATTACTGATGGTGTTGATGTTCTCTCACTTTCACTCGGTCGAGGTTCCATGGAGTTTTTCGACGACGTAATAGCGATTGGAGCCTTCCATGCGGTTCAACAAGGGATGGTCGTCGTCTGCGCAGGTGGAAACTCAGGACCCGATCTACAGAGCGTAGAAAACGTGGCGCCTTGGCTTTTCACTGTGGCTGCTAGCACAATCAACAGACAATTTACTAGTTATGTGGCCCTTGGAAACGAGAAGAATATCTCGGGGTCTCTTGATCCGAGAAAGGTGAAAGGGAAGATTATAGTTTGCGTTAGAGGGGACGATGCAAGAGTGAACAAGGGTTTTGTGGCTGCTAAAGCAGGTGCTGTTGGAATGATTCTAGCCAACAACGAGGAATATGGGAATGATGTTGTAGCCGATGCACACATCCTTCCTGTCTCCCACATAAAGATGGGAATCAAACCTGCACCTGTTATGGCTTCATTCTCATCTAGAGGTCCCAGTTCAATTGAGGAGTCGATACTCAAGCCTGATATAACAGCACCGGGCATCAATATAATTGCAGCCTATTCAAAAGATGCATCACCAAGTGGTTCACCATTTGATAACCGTCGAATTCCATTTAATCTAGTATCTGGCACTTCCATGTCATGTCCGCATATTTCTGGTATTGTTGCCCTTCTCAAGACCATTTATCCAAAATGGAGTCCAGCAGCTATCAAATCTGCAATCATGACCACAGCTGAAACTAGAGCCAATGACTTGCATCCAATACTAGATTTCACCAAGCTTGTAGCCAACCCATTGGCGTACGGTGCGGGACATGTGCAACCCAACCGAGCAGCAAATCCTGGCCTTGTTTATGACCTTACCACCAATGACTATTTAAATTTTTTATGTGCTCGAGGCTACAATAAAGCACAACTCAGTAAATTCTCCAATACGTCATTCGTTTGTTCAAAGTCATTCAAACTAACAGATTTCAACTACCCATCAATCTCGATACCCAATATGAAATCAGGACCTGTGACAATCAAAAGAACAGTTAAGAATGTGGGAAGCCCAAGTACATATGTAGCTCGGGTGGAGGTACCCCCAGGAGTTTTAGTTTCAATTAAGCCAAGTACGTTGAAGTTTACTCGAACTGATGAAGAGAAGACTTTCGAAGTTGTATTCCAAAGTGTGGCAAACAACAAGCATCGAGGCTATGTATTTGGGTCTTTGAAATGGTTAGATGGTAAGCATCATGTAAGAAGCTCAATTGTAGTGAATTTGGGATGA

Protein sequence

MNIGHLVLHCLLGITFARLESVISRSPSRNGISLQFARVIVRKYSNEMAKEAIFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLELENGYEAIPSRWRGSCEGGSNFHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGTAKGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFDDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNEKNISGSLDPRKVKGKIIVCVRGDDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHIKMGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGSPFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKFTRTDEEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVVNLG
BLAST of CmaCh16G009850 vs. Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 5.1e-180
Identity = 349/702 (49.72%), Postives = 443/702 (63.11%), Query Frame = 1

Query: 45  SNEMAKEAIFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLEL 104
           S+E AKEAIFYSY ++INGFAA+LD     ++A+HP VVS+  NK RKLHTT+SW F+ L
Sbjct: 77  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 136

Query: 105 -ENGYEAIPSRWRGSCEG-----------------------------------GSNFHCN 164
            +NG     S W  +  G                                     +  CN
Sbjct: 137 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKDVPCN 196

Query: 165 KKLIGARYFNKGYVALAG-SLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGTA 224
           +KLIGARYFNKGY+A  G   + S++T RD +GHG+HTLSTA G+FV GANVFG GNGTA
Sbjct: 197 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 256

Query: 225 KGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFDDVI 284
            GGSPKARVAAYKVCW    G +C DADILAAIEAAI DGVDVLS S+G  + ++  D I
Sbjct: 257 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 316

Query: 285 AIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNEKNIS 344
           AIG+FHAV+ G+ VVC+ GNSGP   +V NVAPW+ TV AS+++R+F ++V L N ++  
Sbjct: 317 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 376

Query: 345 G-SLD------------------------------------PRKVKGKIIVCVRGDDARV 404
           G SL                                     P+KVKGKI+VC+RGD+ARV
Sbjct: 377 GTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARV 436

Query: 405 NKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI-----------------KMGIK 464
           +KG  AA AGA GM+L N++  GN++++DAH+LP S I                   G  
Sbjct: 437 DKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYI 496

Query: 465 PAPV----------MASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGSPFDNR 524
            AP           MASFSSRGP++I   ILKPDITAPG+NIIAA+++   P+    DNR
Sbjct: 497 KAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNR 556

Query: 525 RIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDFTKL 584
           R PFN  SGTSMSCPHISG+V LLKT++P WSPAAI+SAIMTT+ TR N   P++D +  
Sbjct: 557 RTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFK 616

Query: 585 VANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFS-NTSFVCSKSF 644
            ANP +YG+GHVQPN+AA+PGLVYDLTT DYL+FLCA GYN   +  F+ +  + C +  
Sbjct: 617 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 676

BLAST of CmaCh16G009850 vs. Swiss-Prot
Match: AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.4e-169
Identity = 313/608 (51.48%), Postives = 392/608 (64.47%), Query Frame = 1

Query: 105 ENGYEAIPSRWRGSCEG--GSNFHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHG 164
           + G   IPSRW+G C+    + FHCN+KLIGARYFNKGY A  G L+ SFD+ RD +GHG
Sbjct: 165 DEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHG 224

Query: 165 THTLSTAGGSFVSGANVFGYGNGTAKGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEA 224
           +HTLSTA G FV G ++FG GNGTAKGGSP+ARVAAYKVCW   +G +C DAD+LAA +A
Sbjct: 225 SHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDA 284

Query: 225 AITDGVDVLSLSLGRGSMEFFDDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWL 284
           AI DG DV+S+SLG     FF+D +AIG+FHA ++ +VVVC+ GNSGP   +V NVAPW 
Sbjct: 285 AIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQ 344

Query: 285 FTVAASTINRQFTSYVALGNEKNISG----------------------------SLDPRK 344
            TV AST++R+F S + LGN K+  G                            +LD + 
Sbjct: 345 ITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQL 404

Query: 345 VKGK----------IIVCVRGDDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHIL- 404
            K            I+VC+RG + RV KG   A  G +GM+L N    GND++AD H+L 
Sbjct: 405 CKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLP 464

Query: 405 ---------------------PVSHI-----KMGIKPAPVMASFSSRGPSSIEESILKPD 464
                                P++HI      +G+KPAPVMASFSS+GPS +   ILKPD
Sbjct: 465 ATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPD 524

Query: 465 ITAPGINIIAAYSKDASPSGSPFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPA 524
           ITAPG+++IAAY+   SP+   FD RR+ FN +SGTSMSCPHISGI  LLKT YP WSPA
Sbjct: 525 ITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPA 584

Query: 525 AIKSAIMTTAETRANDLHPILDFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNF 584
           AI+SAIMTTA    +   PI + T + A P ++GAGHVQPN A NPGLVYDL   DYLNF
Sbjct: 585 AIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNF 644

Query: 585 LCARGYNKAQLSKFSNTSFVCSK-SFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPST 644
           LC+ GYN +Q+S FS  +F CS     L + NYPSI++PN+ S  VT+ RTVKNVG PS 
Sbjct: 645 LCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSM 704

BLAST of CmaCh16G009850 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 2.2e-135
Identity = 279/695 (40.14%), Postives = 390/695 (56.12%), Query Frame = 1

Query: 53  IFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLE--------- 112
           + Y+Y   I+GF+  L  +    L   P V+S+      +LHTT +  FL          
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLF 124

Query: 113 -------------------------LENGYEAIPSRWRGSCEGGSNFH---CNKKLIGAR 172
                                     + G+  IPS W+G CE G+NF    CN+KLIGAR
Sbjct: 125 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 184

Query: 173 YFNKGYVALAGSLDVSFDTA--RDQEGHGTHTLSTAGGSFVSGANVFGYGNGTAKGGSPK 232
           +F +GY +  G +D S ++   RD +GHGTHT STA GS V GA++ GY +GTA+G +P+
Sbjct: 185 FFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPR 244

Query: 233 ARVAAYKVCWLTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFDDVIAIGAFH 292
           ARVA YKVCWL    G C  +DILAAI+ AI D V+VLS+SLG G  +++ D +AIGAF 
Sbjct: 245 ARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFA 304

Query: 293 AVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNEKNISG----- 352
           A+++G++V C+ GN+GP   S+ NVAPW+ TV A T++R F +   LGN KN +G     
Sbjct: 305 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 364

Query: 353 ------SLDP--------RKVKGKIIVC-------VRGDDARVNKGFVAAKAGA------ 412
                  L P            G + +        V+G     ++G  A           
Sbjct: 365 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 424

Query: 413 --VGMILANNEEYGNDVVADAHILPVSHI---------------------------KMGI 472
             VGMILAN    G ++VADAH+LP + +                            +G+
Sbjct: 425 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGV 484

Query: 473 KPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGSPFDNRRIPFNLVSG 532
           KP+PV+A+FSSRGP+SI  +ILKPD+ APG+NI+AA++  A P+G   D+RR+ FN++SG
Sbjct: 485 KPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 544

Query: 533 TSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDF-TKLVANPLAYG 592
           TSMSCPH+SG+ ALLK+++P+WSPAAI+SA+MTTA     D  P+LD  T   + P  +G
Sbjct: 545 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 604

Query: 593 AGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVC--SKSFKLTDFNY 644
           AGHV P  A NPGL+YDLTT DYL FLCA  Y   Q+   S  ++ C  SKS+ + D NY
Sbjct: 605 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNY 664

BLAST of CmaCh16G009850 vs. Swiss-Prot
Match: SBT11_ARATH (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 2.8e-122
Identity = 272/700 (38.86%), Postives = 369/700 (52.71%), Query Frame = 1

Query: 53  IFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLELENG----- 112
           I Y Y   ++GF+A L    +  +      +S + ++L  LHTT S EFL LE G     
Sbjct: 79  IHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWN 138

Query: 113 ----------------------------YEAIPSRWRGSCEGGSNF---HCNKKLIGARY 172
                                          +PSRWRGSC+ G+NF    CNKK+IGA  
Sbjct: 139 ETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASA 198

Query: 173 FNKGYVALAGSLD--VSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGTAKGGSPKA 232
           F KGY ++ G ++    F + RD +GHGTHT STA G  V  AN FG   G A G    +
Sbjct: 199 FYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTS 258

Query: 233 RVAAYKVCWLTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFDDVIAIGAFHA 292
           R+AAYK CW       C+  D++AAI+ AI DGVDV+SLSLG  S  F+ D IAI  F A
Sbjct: 259 RIAAYKACWAL----GCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGA 318

Query: 293 VQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTS------------------ 352
           +Q+ + V C+ GNSGP   +V N APWL TVAAS  +R F +                  
Sbjct: 319 MQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKG 378

Query: 353 ---------YVALGNEKN-----ISGSLDPRKVKGKIIVCVRGDDARVNKGFVAAKAGAV 412
                    +     E++     I  SL    V+GKI++C+RG   R  KG    ++G  
Sbjct: 379 KSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSGGA 438

Query: 413 GMILANNEEYGNDVVADAHILPVSHIKMGIK----------------------------P 472
            M+L + E  G +++AD H+LP   + +G                               
Sbjct: 439 AMLLVSTEAEGEELLADPHVLPA--VSLGFSDGKTLLNYLAGAANATASVRFRGTAYGAT 498

Query: 473 APVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGSPFDNRRIPFNLVSGTS 532
           AP++A+FSSRGPS     I KPDI APG+NI+A +S  +SPS    D RR+ FN++SGTS
Sbjct: 499 APMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTS 558

Query: 533 MSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILD----FTKLVANPLAY 592
           M+CPHISGI AL+K+++  WSPA IKSAIMTTA    N   PI D      +  A   A+
Sbjct: 559 MACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAF 618

Query: 593 GAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFKLT---DF 643
           GAG+V P RA +PGLVYD +T DYLN+LC+  Y   ++  FS T++ C+ +  +    D 
Sbjct: 619 GAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDL 678

BLAST of CmaCh16G009850 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.4e-121
Identity = 263/600 (43.83%), Postives = 349/600 (58.17%), Query Frame = 1

Query: 111 IPSRWRGSCEGGSNFH---CNKKLIGARYFNKGYVALAG---SLDVSFDTARDQEGHGTH 170
           IPS+W+G CE GS+F    CNKKLIGAR F+KG+   +G   S      + RD +GHGTH
Sbjct: 152 IPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTH 211

Query: 171 TLSTAGGSFVSGANVFGYGNGTAKGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEAAI 230
           T +TA GS V  A+  GY  GTA+G + +ARVA YKVCW T     C  +DILAA++ AI
Sbjct: 212 TSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWST----GCFGSDILAAMDRAI 271

Query: 231 TDGVDVLSLSLGRGSMEFFDDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFT 290
            DGVDVLSLSLG GS  ++ D IAIGAF A+++G+ V C+ GNSGP   SV NVAPW+ T
Sbjct: 272 LDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMT 331

Query: 291 VAASTINR-----------------QFTSYVALG--------NEKNIS-------GSLDP 350
           V A T++R                    S V +G        N+ N S       GSLD 
Sbjct: 332 VGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDS 391

Query: 351 RKVKGKIIVCVRGDDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPV------- 410
             V+GKI+VC RG +ARV KG V   AG +GMI+AN    G ++VAD+H+LP        
Sbjct: 392 SIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKT 451

Query: 411 -----SHIK---------------MGIKPAPVMASFSSRGPSSIEESILKPDITAPGINI 470
                 ++K               + +KP+PV+A+FSSRGP+++   ILKPD+  PG+NI
Sbjct: 452 GDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNI 511

Query: 471 IAAYSKDASPSGSPFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMT 530
           +A +S    P+G   D+RR  FN++SGTSMSCPHISG+  LLK  +P+WSP+AIKSA+MT
Sbjct: 512 LAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMT 571

Query: 531 TAETRANDLHPILDFT-KLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYN 590
           TA    N   P+ D     ++NP A+G+GHV P +A +PGLVYD++T +Y+ FLC+  Y 
Sbjct: 572 TAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYT 631

Query: 591 KAQL-SKFSNTSFVCSKSFK-LTDFNYPSISIPNMKSGPVTIKRTVKNVGSPST-YVARV 641
              + +     S  CSK F      NYPS S+       V   R V NVG+ S+ Y   V
Sbjct: 632 VDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTV 691

BLAST of CmaCh16G009850 vs. TrEMBL
Match: A0A0A0L2R9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 1.1e-226
Identity = 425/710 (59.86%), Postives = 502/710 (70.70%), Query Frame = 1

Query: 45  SNEMAKEAIFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLEL 104
           SNE AKEAIFYSYN++INGFAAV+D KV +DLA+HP VVS+ ENK RKLHTTNSW+FL L
Sbjct: 52  SNEEAKEAIFYSYNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLHTTNSWKFLGL 111

Query: 105 ENG--------------------------------------YEAIPSRWRGSCEGGSNFH 164
           EN                                       Y  IPSRW+GSCEGGS F+
Sbjct: 112 ENNGAIPSNSLWNLASFGESTIIGNLDTGVWPESKSFSDKEYGPIPSRWKGSCEGGSKFY 171

Query: 165 CNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGT 224
           CN+KLIGARY+NKGY A+ G L+ S+++ARD EGHGTHTLSTAGG FV  AN+FGYGNGT
Sbjct: 172 CNRKLIGARYYNKGYAAIVGPLNSSYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGT 231

Query: 225 AKGGSPKARVAAYKVCW---LTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFF 284
           AKGGSPKA VAAYKVCW   L F  G+C DADILA  EAAI DGVDVLS+SLG    +F 
Sbjct: 232 AKGGSPKALVAAYKVCWPQVLFF--GECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFA 291

Query: 285 DDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNE 344
            D I+IG+FHAVQ G+VVVC+ GNSGP   SV NVAPW+ TV AST +R +TSYVA+G++
Sbjct: 292 KDSISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDK 351

Query: 345 KNISG--------------------------------------SLDPRKVKGKIIVCVRG 404
           ++  G                                      SLDP+KV GKII+C+RG
Sbjct: 352 RHFKGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRG 411

Query: 405 DDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI----------------- 464
           D+ARV KG+VAAKAGAVGMILAN EE G++++ADAH+LP SHI                 
Sbjct: 412 DNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKI 471

Query: 465 ----------KMGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGS 524
                     + GIKPAPVMASFSSRGP++++ SILKPDITAPG NI+AAYS+DASP+G+
Sbjct: 472 PMAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGT 531

Query: 525 PFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPIL 584
            FD RR+PFN+ SGTSMSCPH+SGIV LLKT+YPKWSPAAI+SAIMTTA T+ANDL PIL
Sbjct: 532 DFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPIL 591

Query: 585 DFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSN-TSFV 644
              +  AN  AYGAGHV PNRAA+PGLVYDL+T DYLN+LCA+GYN AQ+ +FSN TSFV
Sbjct: 592 STNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFV 651

Query: 645 CSKSFKLTDFNYPSISIPNMK-SGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTL 646
           CSKSFKLTD NYPSISIP ++    V IKR +KNVGSP TYV +V+ P GV VS++P++L
Sbjct: 652 CSKSFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSL 711

BLAST of CmaCh16G009850 vs. TrEMBL
Match: A0A0A0L613_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045110 PE=4 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 4.9e-214
Identity = 395/587 (67.29%), Postives = 450/587 (76.66%), Query Frame = 1

Query: 125 FHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGN 184
           F   +KLIGA+YFNKGY A AGSL+ S++TARD EGHGTHTLSTAGG+F+SGANVFG GN
Sbjct: 46  FQKYRKLIGAKYFNKGYAANAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGN 105

Query: 185 GTAKGGSPKARVAAYKVCWLTF-RGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFF 244
           GTAKGGSPKA VAAYKVCW     GG C DADILAA+EAAI+DGVDVLSLSLG GS +F 
Sbjct: 106 GTAKGGSPKALVAAYKVCWPQVDSGGGCYDADILAAMEAAISDGVDVLSLSLGGGSKDFS 165

Query: 245 DDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNE 304
           DDV AIGAFHAVQQG+VVVC+ GNSGP   +VENVAPW+ TV ASTINR FTS+V+LGN+
Sbjct: 166 DDVTAIGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIITVGASTINRDFTSFVSLGNK 225

Query: 305 KNISGSLDPRKV--KGKIIVCVRGDDARVN------------------------------ 364
           K+I G+    K+  + K    +   DA+ N                              
Sbjct: 226 KHIKGASLSDKILPEQKFYPLIDAVDAKANKVSSDIAQLCLVGSLDPEKVKGKIIICLRG 285

Query: 365 ------KGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI----------------- 424
                 KG+ A KAGAVGMILAN EE G++++ADAH+LPVSH+                 
Sbjct: 286 ENARADKGYAAVKAGAVGMILANAEENGDEIIADAHLLPVSHVSYTDGQSIYQYINSTKT 345

Query: 425 ----------KMGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGS 484
                     ++GIKPAPVMASFSSRGP+++EE+ILKPDITAPG+NI+AAYS+DASPSGS
Sbjct: 346 PMAYMTHVRTELGIKPAPVMASFSSRGPNTVEETILKPDITAPGVNILAAYSEDASPSGS 405

Query: 485 PFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPIL 544
            FD RRIPFN+VSGTSMSCPHISGIV LLKT+YP WSPAAIKSAIMTTAE+RAND+HPI 
Sbjct: 406 FFDKRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAESRANDMHPIQ 465

Query: 545 DFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVC 604
           +   L AN  AYGAGHVQPNRA NPGLVYDLTT DY+NFLCA+GYNK+Q+SKFS  SFVC
Sbjct: 466 NGGNLKANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLCAQGYNKSQISKFSAMSFVC 525

Query: 605 SKSFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKF 646
           SKSFKLTDFNYPSISIP+MKSG VTIKR VKNVG PSTYVARV+VP GV VS++P TLKF
Sbjct: 526 SKSFKLTDFNYPSISIPDMKSGVVTIKRRVKNVGKPSTYVARVKVPHGVSVSVEPRTLKF 585

BLAST of CmaCh16G009850 vs. TrEMBL
Match: A0A0D2U544_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G453100 PE=4 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 1.3e-193
Identity = 367/708 (51.84%), Postives = 462/708 (65.25%), Query Frame = 1

Query: 45  SNEMAKEAIFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSW----- 104
           S E AKE IFYSY + INGFAA+LD      +++HP VVS+  NK RKLHTT SW     
Sbjct: 65  SPETAKEKIFYSYTRNINGFAAILDEDEASQVSKHPNVVSVFLNKGRKLHTTRSWDFLRL 124

Query: 105 ---------------EFLE------------------LENGYEAIPSRWRGSCEGG--SN 164
                          +F E                   ++G   IPSRWRGSC+ G    
Sbjct: 125 ERNGVIPSDSLWKKAKFGEDTIIGNLDTGVWPESKSFSDDGIGPIPSRWRGSCQRGVDDR 184

Query: 165 FHCNKKLIGARYFNKGYVALAGS-LDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYG 224
           F CN+KLIGA+YFN+GY A  G  L+ +F T RD EGHG+HTLSTAGG+FV GA+VFGYG
Sbjct: 185 FRCNRKLIGAKYFNRGYKAYLGEKLNATFKTVRDHEGHGSHTLSTAGGNFVDGASVFGYG 244

Query: 225 NGTAKGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFF 284
           NGTAKGGSPKARVAAYKVCW    G QC DADI+AA +AAI+DGVDVLS+SLG    EFF
Sbjct: 245 NGTAKGGSPKARVAAYKVCWPPVNGNQCFDADIMAAFDAAISDGVDVLSVSLGGEPAEFF 304

Query: 285 DDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNE 344
           +D IAIG+FHAV++G+ VV +GGNSGP   +V N++PW+FTV AST++R+FTSYV LGN+
Sbjct: 305 EDGIAIGSFHAVKKGISVVLSGGNSGPAPGTVSNLSPWMFTVGASTLDREFTSYVELGNK 364

Query: 345 KNISG--------------------------------------SLDPRKVKGKIIVCVRG 404
             + G                                      SLDP KVKGKI+VC+RG
Sbjct: 365 IRLKGASLAAATLKSKTSYPLIGADCAKAANASAVDAILCQPGSLDPTKVKGKILVCLRG 424

Query: 405 DDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI----------------- 464
            +AR +KG  A  AGAVGMILAN+E+ GN+V+AD H+LP +H+                 
Sbjct: 425 INARTDKGKQALLAGAVGMILANDEKSGNEVIADPHLLPATHLNFTDGVTVFAYINSTKK 484

Query: 465 ----------KMGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGS 524
                     ++  KPAP MASFSSRGP+ I+ +ILKPDITAPG++IIAA+S+   P+  
Sbjct: 485 PTAYLTPVKTQLDAKPAPFMASFSSRGPNMIDPAILKPDITAPGVSIIAAFSESVGPTED 544

Query: 525 PFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPIL 584
             D RRIPF   SGTSMSCPH+SGIV LLK+++P+WSPAAI+SAIMTTA TR N ++P+L
Sbjct: 545 ESDTRRIPFTSQSGTSMSCPHVSGIVGLLKSLHPEWSPAAIRSAIMTTARTRDNTVNPML 604

Query: 585 DFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVC 643
           D     A P +YGAGHV+PNRA +PGL+YDLT +DY+N+LCARGYN+  +  FSN  +VC
Sbjct: 605 DSDGNKATPFSYGAGHVRPNRAMDPGLIYDLTIDDYVNYLCARGYNQTTIQMFSNKPYVC 664

BLAST of CmaCh16G009850 vs. TrEMBL
Match: A0A0A0L601_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045060 PE=4 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 1.0e-187
Identity = 342/605 (56.53%), Postives = 414/605 (68.43%), Query Frame = 1

Query: 107 GYEAIPSRWRGSCEGGSNFHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTL 166
           GY  +PSRWRG+CEGG+NF CN+KLIGARYFNKG+   +G L++SF+TARD++GHG+HTL
Sbjct: 142 GYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNISFNTARDKQGHGSHTL 201

Query: 167 STAGGSFVSGANVFGYGNGTAKGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEAAITD 226
           STAGG+FV GANVFGYGNGTAKGGSPKARVAAYKVCW    GG C DADILA  EAAI+D
Sbjct: 202 STAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADILAGFEAAISD 261

Query: 227 GVDVLSLSLGRGSMEFFDDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVA 286
           GVDVLS+SLG    EF  D ++IGAFHAVQQG+VVVC+ GN GP   +V N++PW+FTVA
Sbjct: 262 GVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVSNISPWMFTVA 321

Query: 287 ASTINRQFTSYVALGNEKNISGS--LDPRKVKGKIIVCVRGDDARV-------------- 346
           AS+I+R FTSY +LGN+K+  GS         GK    +   DA+               
Sbjct: 322 ASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAVDAKAANASEILAQLCHKG 381

Query: 347 ----------------------NKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI 406
                                  KGFV  +AG VGMIL N +  G+   ADAHILP +H+
Sbjct: 382 SLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHL 441

Query: 407 ---------------------------KMGIKPAPVMASFSSRGPSSIEESILKPDITAP 466
                                      ++GIKP+PVMA FSSRGP+ I E++LKPDIT P
Sbjct: 442 SYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITEAMLKPDITGP 501

Query: 467 GINIIAAYSKDASPSGSPFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKS 526
           G++I+A+ + D + +  PFD RR+PFN+ SGTSMSCPHISG+V LLKT+YP WSPAAIKS
Sbjct: 502 GMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKS 561

Query: 527 AIMTTAETRANDLHPILDFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCAR 586
           AIMTTA+TR N +  I D  K  A P  YGAGHV PN A +PGLVYD T +DYLNFLCAR
Sbjct: 562 AIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFLCAR 621

Query: 587 GYNKAQLSKFSNTSFVCSKSFKLTDFNYPSISIPNMKSG-PVTIKRTVKNVGSPSTYVAR 646
           GYN      F N  FVC+KSF LTD NYPSISIP ++ G PVT+ R VKNVG+P TYVAR
Sbjct: 622 GYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKNVGTPGTYVAR 681

BLAST of CmaCh16G009850 vs. TrEMBL
Match: A0A059BWH5_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03662 PE=4 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.8e-187
Identity = 359/703 (51.07%), Postives = 447/703 (63.58%), Query Frame = 1

Query: 45  SNEMAKEAIFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLEL 104
           SNE A+EAIFYSY  +INGFAA+LD     ++A+HP V+S+  +K RKLHTT SW FL L
Sbjct: 65  SNEKAQEAIFYSYTHHINGFAAILDEDQAAEVAKHPDVLSVFLSKGRKLHTTRSWHFLGL 124

Query: 105 EN--------------------------------------GYEAIPSRWRGSCEGG--SN 164
           E                                       G   IP +WRG C+ G    
Sbjct: 125 ERNGVIHKNSILRKARFGEDTIIGNLDTGVWPESKSFSDEGMGPIPGKWRGICQRGIKDG 184

Query: 165 FHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGN 224
           F CN+KLIGARYFNKGY A AG L+ +F TARD +GHG+HTLSTAGGS V GA+VFGYGN
Sbjct: 185 FSCNRKLIGARYFNKGYAAYAGLLNSTFYTARDHDGHGSHTLSTAGGSLVPGASVFGYGN 244

Query: 225 GTAKGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFD 284
           GT+KGGSPKARV AYKVCW       C DAD+LA  +AAI+DGVDVLS+SLG    EFF 
Sbjct: 245 GTSKGGSPKARVVAYKVCWPPLEDVGCFDADVLAGFDAAISDGVDVLSVSLGDEPAEFFM 304

Query: 285 DVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNEK 344
           D I IG+FHA + G+VVV + GNSGP   +V NVAPW+ TVAASTI+R+FT+Y+ALGN++
Sbjct: 305 DGIVIGSFHAARNGIVVVSSAGNSGPQPGTVLNVAPWMITVAASTIDREFTTYIALGNKQ 364

Query: 345 NISG-SLDPRKV-KGKIIVCVRGDDA---------------------------------- 404
           ++ G SL   ++   K    + G DA                                  
Sbjct: 365 HLKGSSLSANELPSNKFYPLISGADAQAANVSAAEALLCKAGTLDRKKVKGKILACLLVD 424

Query: 405 --RVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHIK----------------- 464
             R+  G  AA AGAVGM+LAN+E  GNDVVADAH+LP +HI                  
Sbjct: 425 NKRIVVGQQAALAGAVGMVLANDEANGNDVVADAHVLPAAHISFVDGKVLFAYINSTAFP 484

Query: 465 ----------MGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGSP 524
                     +   PAP MASFSSRGP+ +E +ILKPDITAPG+NIIAAY+    P+G  
Sbjct: 485 MAYITRVKTMLNTTPAPFMASFSSRGPNIVEPAILKPDITAPGLNIIAAYTLAVGPAGQD 544

Query: 525 FDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILD 584
           FD R+ PF+  SGTSMSCPH++GIV LLKT+YP WSPAAI+SAIMTTA TR N L P+LD
Sbjct: 545 FDKRKTPFSAESGTSMSCPHVAGIVGLLKTLYPSWSPAAIQSAIMTTARTRDNKLEPMLD 604

Query: 585 FTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCS 643
            +   A P +YGAGH++PNRA NPGLVYDLT NDYL+FLCARGYN++QL  F+N +  C 
Sbjct: 605 ASFAKATPFSYGAGHIRPNRAMNPGLVYDLTANDYLDFLCARGYNESQLRLFTNETHRCP 664

BLAST of CmaCh16G009850 vs. TAIR10
Match: AT5G59810.1 (AT5G59810.1 Subtilase family protein)

HSP 1 Score: 632.5 bits (1630), Expect = 2.9e-181
Identity = 349/702 (49.72%), Postives = 443/702 (63.11%), Query Frame = 1

Query: 45  SNEMAKEAIFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLEL 104
           S+E AKEAIFYSY ++INGFAA+LD     ++A+HP VVS+  NK RKLHTT+SW F+ L
Sbjct: 77  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 136

Query: 105 -ENGYEAIPSRWRGSCEG-----------------------------------GSNFHCN 164
            +NG     S W  +  G                                     +  CN
Sbjct: 137 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKDVPCN 196

Query: 165 KKLIGARYFNKGYVALAG-SLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGTA 224
           +KLIGARYFNKGY+A  G   + S++T RD +GHG+HTLSTA G+FV GANVFG GNGTA
Sbjct: 197 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 256

Query: 225 KGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFDDVI 284
            GGSPKARVAAYKVCW    G +C DADILAAIEAAI DGVDVLS S+G  + ++  D I
Sbjct: 257 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 316

Query: 285 AIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNEKNIS 344
           AIG+FHAV+ G+ VVC+ GNSGP   +V NVAPW+ TV AS+++R+F ++V L N ++  
Sbjct: 317 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 376

Query: 345 G-SLD------------------------------------PRKVKGKIIVCVRGDDARV 404
           G SL                                     P+KVKGKI+VC+RGD+ARV
Sbjct: 377 GTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARV 436

Query: 405 NKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI-----------------KMGIK 464
           +KG  AA AGA GM+L N++  GN++++DAH+LP S I                   G  
Sbjct: 437 DKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYI 496

Query: 465 PAPV----------MASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGSPFDNR 524
            AP           MASFSSRGP++I   ILKPDITAPG+NIIAA+++   P+    DNR
Sbjct: 497 KAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNR 556

Query: 525 RIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDFTKL 584
           R PFN  SGTSMSCPHISG+V LLKT++P WSPAAI+SAIMTT+ TR N   P++D +  
Sbjct: 557 RTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFK 616

Query: 585 VANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFS-NTSFVCSKSF 644
            ANP +YG+GHVQPN+AA+PGLVYDLTT DYL+FLCA GYN   +  F+ +  + C +  
Sbjct: 617 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 676

BLAST of CmaCh16G009850 vs. TAIR10
Match: AT2G04160.1 (AT2G04160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 597.8 bits (1540), Expect = 7.8e-171
Identity = 313/608 (51.48%), Postives = 392/608 (64.47%), Query Frame = 1

Query: 105 ENGYEAIPSRWRGSCEG--GSNFHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHG 164
           + G   IPSRW+G C+    + FHCN+KLIGARYFNKGY A  G L+ SFD+ RD +GHG
Sbjct: 165 DEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHG 224

Query: 165 THTLSTAGGSFVSGANVFGYGNGTAKGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEA 224
           +HTLSTA G FV G ++FG GNGTAKGGSP+ARVAAYKVCW   +G +C DAD+LAA +A
Sbjct: 225 SHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDA 284

Query: 225 AITDGVDVLSLSLGRGSMEFFDDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWL 284
           AI DG DV+S+SLG     FF+D +AIG+FHA ++ +VVVC+ GNSGP   +V NVAPW 
Sbjct: 285 AIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQ 344

Query: 285 FTVAASTINRQFTSYVALGNEKNISG----------------------------SLDPRK 344
            TV AST++R+F S + LGN K+  G                            +LD + 
Sbjct: 345 ITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQL 404

Query: 345 VKGK----------IIVCVRGDDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHIL- 404
            K            I+VC+RG + RV KG   A  G +GM+L N    GND++AD H+L 
Sbjct: 405 CKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLP 464

Query: 405 ---------------------PVSHI-----KMGIKPAPVMASFSSRGPSSIEESILKPD 464
                                P++HI      +G+KPAPVMASFSS+GPS +   ILKPD
Sbjct: 465 ATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPD 524

Query: 465 ITAPGINIIAAYSKDASPSGSPFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPA 524
           ITAPG+++IAAY+   SP+   FD RR+ FN +SGTSMSCPHISGI  LLKT YP WSPA
Sbjct: 525 ITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPA 584

Query: 525 AIKSAIMTTAETRANDLHPILDFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNF 584
           AI+SAIMTTA    +   PI + T + A P ++GAGHVQPN A NPGLVYDL   DYLNF
Sbjct: 585 AIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNF 644

Query: 585 LCARGYNKAQLSKFSNTSFVCSK-SFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPST 644
           LC+ GYN +Q+S FS  +F CS     L + NYPSI++PN+ S  VT+ RTVKNVG PS 
Sbjct: 645 LCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSM 704

BLAST of CmaCh16G009850 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 484.2 bits (1245), Expect = 1.3e-136
Identity = 279/695 (40.14%), Postives = 390/695 (56.12%), Query Frame = 1

Query: 53  IFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLE--------- 112
           + Y+Y   I+GF+  L  +    L   P V+S+      +LHTT +  FL          
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLF 124

Query: 113 -------------------------LENGYEAIPSRWRGSCEGGSNFH---CNKKLIGAR 172
                                     + G+  IPS W+G CE G+NF    CN+KLIGAR
Sbjct: 125 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 184

Query: 173 YFNKGYVALAGSLDVSFDTA--RDQEGHGTHTLSTAGGSFVSGANVFGYGNGTAKGGSPK 232
           +F +GY +  G +D S ++   RD +GHGTHT STA GS V GA++ GY +GTA+G +P+
Sbjct: 185 FFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPR 244

Query: 233 ARVAAYKVCWLTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFDDVIAIGAFH 292
           ARVA YKVCWL    G C  +DILAAI+ AI D V+VLS+SLG G  +++ D +AIGAF 
Sbjct: 245 ARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFA 304

Query: 293 AVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNEKNISG----- 352
           A+++G++V C+ GN+GP   S+ NVAPW+ TV A T++R F +   LGN KN +G     
Sbjct: 305 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 364

Query: 353 ------SLDP--------RKVKGKIIVC-------VRGDDARVNKGFVAAKAGA------ 412
                  L P            G + +        V+G     ++G  A           
Sbjct: 365 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 424

Query: 413 --VGMILANNEEYGNDVVADAHILPVSHI---------------------------KMGI 472
             VGMILAN    G ++VADAH+LP + +                            +G+
Sbjct: 425 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGV 484

Query: 473 KPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGSPFDNRRIPFNLVSG 532
           KP+PV+A+FSSRGP+SI  +ILKPD+ APG+NI+AA++  A P+G   D+RR+ FN++SG
Sbjct: 485 KPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 544

Query: 533 TSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDF-TKLVANPLAYG 592
           TSMSCPH+SG+ ALLK+++P+WSPAAI+SA+MTTA     D  P+LD  T   + P  +G
Sbjct: 545 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 604

Query: 593 AGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVC--SKSFKLTDFNY 644
           AGHV P  A NPGL+YDLTT DYL FLCA  Y   Q+   S  ++ C  SKS+ + D NY
Sbjct: 605 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNY 664

BLAST of CmaCh16G009850 vs. TAIR10
Match: AT1G01900.1 (AT1G01900.1 subtilase family protein)

HSP 1 Score: 440.7 bits (1132), Expect = 1.6e-123
Identity = 272/700 (38.86%), Postives = 369/700 (52.71%), Query Frame = 1

Query: 53  IFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLELENG----- 112
           I Y Y   ++GF+A L    +  +      +S + ++L  LHTT S EFL LE G     
Sbjct: 79  IHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWN 138

Query: 113 ----------------------------YEAIPSRWRGSCEGGSNF---HCNKKLIGARY 172
                                          +PSRWRGSC+ G+NF    CNKK+IGA  
Sbjct: 139 ETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASA 198

Query: 173 FNKGYVALAGSLD--VSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGTAKGGSPKA 232
           F KGY ++ G ++    F + RD +GHGTHT STA G  V  AN FG   G A G    +
Sbjct: 199 FYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTS 258

Query: 233 RVAAYKVCWLTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFDDVIAIGAFHA 292
           R+AAYK CW       C+  D++AAI+ AI DGVDV+SLSLG  S  F+ D IAI  F A
Sbjct: 259 RIAAYKACWAL----GCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGA 318

Query: 293 VQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTS------------------ 352
           +Q+ + V C+ GNSGP   +V N APWL TVAAS  +R F +                  
Sbjct: 319 MQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKG 378

Query: 353 ---------YVALGNEKN-----ISGSLDPRKVKGKIIVCVRGDDARVNKGFVAAKAGAV 412
                    +     E++     I  SL    V+GKI++C+RG   R  KG    ++G  
Sbjct: 379 KSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSGGA 438

Query: 413 GMILANNEEYGNDVVADAHILPVSHIKMGIK----------------------------P 472
            M+L + E  G +++AD H+LP   + +G                               
Sbjct: 439 AMLLVSTEAEGEELLADPHVLPA--VSLGFSDGKTLLNYLAGAANATASVRFRGTAYGAT 498

Query: 473 APVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGSPFDNRRIPFNLVSGTS 532
           AP++A+FSSRGPS     I KPDI APG+NI+A +S  +SPS    D RR+ FN++SGTS
Sbjct: 499 APMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTS 558

Query: 533 MSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILD----FTKLVANPLAY 592
           M+CPHISGI AL+K+++  WSPA IKSAIMTTA    N   PI D      +  A   A+
Sbjct: 559 MACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAF 618

Query: 593 GAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFKLT---DF 643
           GAG+V P RA +PGLVYD +T DYLN+LC+  Y   ++  FS T++ C+ +  +    D 
Sbjct: 619 GAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDL 678

BLAST of CmaCh16G009850 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 438.3 bits (1126), Expect = 8.0e-123
Identity = 263/600 (43.83%), Postives = 349/600 (58.17%), Query Frame = 1

Query: 111 IPSRWRGSCEGGSNFH---CNKKLIGARYFNKGYVALAG---SLDVSFDTARDQEGHGTH 170
           IPS+W+G CE GS+F    CNKKLIGAR F+KG+   +G   S      + RD +GHGTH
Sbjct: 152 IPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTH 211

Query: 171 TLSTAGGSFVSGANVFGYGNGTAKGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEAAI 230
           T +TA GS V  A+  GY  GTA+G + +ARVA YKVCW T     C  +DILAA++ AI
Sbjct: 212 TSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWST----GCFGSDILAAMDRAI 271

Query: 231 TDGVDVLSLSLGRGSMEFFDDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFT 290
            DGVDVLSLSLG GS  ++ D IAIGAF A+++G+ V C+ GNSGP   SV NVAPW+ T
Sbjct: 272 LDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMT 331

Query: 291 VAASTINR-----------------QFTSYVALG--------NEKNIS-------GSLDP 350
           V A T++R                    S V +G        N+ N S       GSLD 
Sbjct: 332 VGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDS 391

Query: 351 RKVKGKIIVCVRGDDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPV------- 410
             V+GKI+VC RG +ARV KG V   AG +GMI+AN    G ++VAD+H+LP        
Sbjct: 392 SIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKT 451

Query: 411 -----SHIK---------------MGIKPAPVMASFSSRGPSSIEESILKPDITAPGINI 470
                 ++K               + +KP+PV+A+FSSRGP+++   ILKPD+  PG+NI
Sbjct: 452 GDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNI 511

Query: 471 IAAYSKDASPSGSPFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMT 530
           +A +S    P+G   D+RR  FN++SGTSMSCPHISG+  LLK  +P+WSP+AIKSA+MT
Sbjct: 512 LAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMT 571

Query: 531 TAETRANDLHPILDFT-KLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYN 590
           TA    N   P+ D     ++NP A+G+GHV P +A +PGLVYD++T +Y+ FLC+  Y 
Sbjct: 572 TAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYT 631

Query: 591 KAQL-SKFSNTSFVCSKSFK-LTDFNYPSISIPNMKSGPVTIKRTVKNVGSPST-YVARV 641
              + +     S  CSK F      NYPS S+       V   R V NVG+ S+ Y   V
Sbjct: 632 VDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTV 691

BLAST of CmaCh16G009850 vs. NCBI nr
Match: gi|659096606|ref|XP_008449186.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103491136 [Cucumis melo])

HSP 1 Score: 833.9 bits (2153), Expect = 1.8e-238
Identity = 445/705 (63.12%), Postives = 516/705 (73.19%), Query Frame = 1

Query: 45  SNEMAKEAIFYSYNKYINGFA------------------AVLDHKVMQ------------ 104
           SNE AKEAIFYSYN++INGFA                  ++ ++K  +            
Sbjct: 67  SNEAAKEAIFYSYNRHINGFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGV 126

Query: 105 ---DLARHPAV---VSIHENKLRKLHTTNSWEFLELEN--GYEAIPSRWRGSCEGGSNFH 164
              D   H ++    S  E+ +     T  W   +  N  GY  +P+RW+GSCEGGS FH
Sbjct: 127 EHDDGIPHNSIWNRASFGESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFH 186

Query: 165 CNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGT 224
           CN+KLIGARYFNKGY A AGSL+ S++TARD EGHGTHTLSTAGG+F+SGANVFG GNGT
Sbjct: 187 CNRKLIGARYFNKGYAAYAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGT 246

Query: 225 AKGGSPKARVAAYKVCW-LTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFFDD 284
           AKGGSPKA VAAYKVCW     GG C DADILAAIEAAI+DGVD+LSLSLG G+ +F +D
Sbjct: 247 AKGGSPKALVAAYKVCWPQVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSED 306

Query: 285 VIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNEKN 344
           V AIGAFHAVQQG++VVC+ GNSGP   ++EN APW+ TV ASTINR FTSYVALGN+K+
Sbjct: 307 VTAIGAFHAVQQGIIVVCSAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKH 366

Query: 345 ISGSLDPRKVKGK--------------------------------------IIVCVRGDD 404
           I G+    K+  +                                      II+C+RG++
Sbjct: 367 IKGASLSDKILPEQKFYPLINAADAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGEN 426

Query: 405 ARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI------------------- 464
           ARV+KG+ AA+AGAVGMILAN E+ G++++ADAH+LPVSH+                   
Sbjct: 427 ARVDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPM 486

Query: 465 --------KMGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGSPF 524
                   ++GIKPAPVMASFSSRGP++IEESILKPDITAPG+NI+AAYS+DASPSGS F
Sbjct: 487 AYMTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLF 546

Query: 525 DNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDF 584
           DNRRIPFN+VSGTSMSCPHISGIV LLKT+YP WSPAAIKSAIMTTAETRANDLHPIL+ 
Sbjct: 547 DNRRIPFNIVSGTSMSCPHISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILNT 606

Query: 585 TKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSK 644
             L ANP AYGAGHVQPNRA NPGLVYDLTTNDY+NFLCA+GYNK+Q+SKFS TSFVCSK
Sbjct: 607 VNLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSK 666

Query: 645 SFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKFTR 646
           SFKLTDFNYPSISIP+MKSG VTI R VKNVG PSTYVARV+VP GV VS++P TLKFT 
Sbjct: 667 SFKLTDFNYPSISIPDMKSGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTG 726

BLAST of CmaCh16G009850 vs. NCBI nr
Match: gi|778675733|ref|XP_011650462.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 800.0 bits (2065), Expect = 3.0e-228
Identity = 428/714 (59.94%), Postives = 505/714 (70.73%), Query Frame = 1

Query: 41  VRKYSNEMAKEAIFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWE 100
           V KYSNE AKEAIFYSYN++INGFAAV+D KV +DLA+HP VVS+ ENK RKLHTTNSW+
Sbjct: 7   VSKYSNEEAKEAIFYSYNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLHTTNSWK 66

Query: 101 FLELENG--------------------------------------YEAIPSRWRGSCEGG 160
           FL LEN                                       Y  IPSRW+GSCEGG
Sbjct: 67  FLGLENNGAIPSNSLWNLASFGESTIIGNLDTGVWPESKSFSDKEYGPIPSRWKGSCEGG 126

Query: 161 SNFHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGY 220
           S F+CN+KLIGARY+NKGY A+ G L+ S+++ARD EGHGTHTLSTAGG FV  AN+FGY
Sbjct: 127 SKFYCNRKLIGARYYNKGYAAIVGPLNSSYESARDHEGHGTHTLSTAGGHFVPNANLFGY 186

Query: 221 GNGTAKGGSPKARVAAYKVCW---LTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGS 280
           GNGTAKGGSPKA VAAYKVCW   L F  G+C DADILA  EAAI DGVDVLS+SLG   
Sbjct: 187 GNGTAKGGSPKALVAAYKVCWPQVLFF--GECFDADILAGFEAAIGDGVDVLSVSLGGSP 246

Query: 281 MEFFDDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVA 340
            +F  D I+IG+FHAVQ G+VVVC+ GNSGP   SV NVAPW+ TV AST +R +TSYVA
Sbjct: 247 SDFAKDSISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVA 306

Query: 341 LGNEKNISG--------------------------------------SLDPRKVKGKIIV 400
           +G++++  G                                      SLDP+KV GKII+
Sbjct: 307 IGDKRHFKGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIII 366

Query: 401 CVRGDDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI------------- 460
           C+RGD+ARV KG+VAAKAGAVGMILAN EE G++++ADAH+LP SHI             
Sbjct: 367 CLRGDNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYIN 426

Query: 461 --------------KMGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDAS 520
                         + GIKPAPVMASFSSRGP++++ SILKPDITAPG NI+AAYS+DAS
Sbjct: 427 STKIPMAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDAS 486

Query: 521 PSGSPFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDL 580
           P+G+ FD RR+PFN+ SGTSMSCPH+SGIV LLKT+YPKWSPAAI+SAIMTTA T+ANDL
Sbjct: 487 PTGTDFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDL 546

Query: 581 HPILDFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSN- 640
            PIL   +  AN  AYGAGHV PNRAA+PGLVYDL+T DYLN+LCA+GYN AQ+ +FSN 
Sbjct: 547 TPILSTNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSND 606

Query: 641 TSFVCSKSFKLTDFNYPSISIPNMK-SGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIK 646
           TSFVCSKSFKLTD NYPSISIP ++    V IKR +KNVGSP TYV +V+ P GV VS++
Sbjct: 607 TSFVCSKSFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVE 666

BLAST of CmaCh16G009850 vs. NCBI nr
Match: gi|700200865|gb|KGN55998.1| (hypothetical protein Csa_3G045120 [Cucumis sativus])

HSP 1 Score: 794.3 bits (2050), Expect = 1.6e-226
Identity = 425/710 (59.86%), Postives = 502/710 (70.70%), Query Frame = 1

Query: 45  SNEMAKEAIFYSYNKYINGFAAVLDHKVMQDLARHPAVVSIHENKLRKLHTTNSWEFLEL 104
           SNE AKEAIFYSYN++INGFAAV+D KV +DLA+HP VVS+ ENK RKLHTTNSW+FL L
Sbjct: 52  SNEEAKEAIFYSYNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLHTTNSWKFLGL 111

Query: 105 ENG--------------------------------------YEAIPSRWRGSCEGGSNFH 164
           EN                                       Y  IPSRW+GSCEGGS F+
Sbjct: 112 ENNGAIPSNSLWNLASFGESTIIGNLDTGVWPESKSFSDKEYGPIPSRWKGSCEGGSKFY 171

Query: 165 CNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGT 224
           CN+KLIGARY+NKGY A+ G L+ S+++ARD EGHGTHTLSTAGG FV  AN+FGYGNGT
Sbjct: 172 CNRKLIGARYYNKGYAAIVGPLNSSYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGT 231

Query: 225 AKGGSPKARVAAYKVCW---LTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFF 284
           AKGGSPKA VAAYKVCW   L F  G+C DADILA  EAAI DGVDVLS+SLG    +F 
Sbjct: 232 AKGGSPKALVAAYKVCWPQVLFF--GECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFA 291

Query: 285 DDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNE 344
            D I+IG+FHAVQ G+VVVC+ GNSGP   SV NVAPW+ TV AST +R +TSYVA+G++
Sbjct: 292 KDSISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDK 351

Query: 345 KNISG--------------------------------------SLDPRKVKGKIIVCVRG 404
           ++  G                                      SLDP+KV GKII+C+RG
Sbjct: 352 RHFKGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRG 411

Query: 405 DDARVNKGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI----------------- 464
           D+ARV KG+VAAKAGAVGMILAN EE G++++ADAH+LP SHI                 
Sbjct: 412 DNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKI 471

Query: 465 ----------KMGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGS 524
                     + GIKPAPVMASFSSRGP++++ SILKPDITAPG NI+AAYS+DASP+G+
Sbjct: 472 PMAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGT 531

Query: 525 PFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPIL 584
            FD RR+PFN+ SGTSMSCPH+SGIV LLKT+YPKWSPAAI+SAIMTTA T+ANDL PIL
Sbjct: 532 DFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPIL 591

Query: 585 DFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSN-TSFV 644
              +  AN  AYGAGHV PNRAA+PGLVYDL+T DYLN+LCA+GYN AQ+ +FSN TSFV
Sbjct: 592 STNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFV 651

Query: 645 CSKSFKLTDFNYPSISIPNMK-SGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTL 646
           CSKSFKLTD NYPSISIP ++    V IKR +KNVGSP TYV +V+ P GV VS++P++L
Sbjct: 652 CSKSFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSL 711

BLAST of CmaCh16G009850 vs. NCBI nr
Match: gi|700200864|gb|KGN55997.1| (hypothetical protein Csa_3G045110 [Cucumis sativus])

HSP 1 Score: 752.3 bits (1941), Expect = 7.1e-214
Identity = 395/587 (67.29%), Postives = 450/587 (76.66%), Query Frame = 1

Query: 125 FHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGN 184
           F   +KLIGA+YFNKGY A AGSL+ S++TARD EGHGTHTLSTAGG+F+SGANVFG GN
Sbjct: 46  FQKYRKLIGAKYFNKGYAANAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGN 105

Query: 185 GTAKGGSPKARVAAYKVCWLTF-RGGQCSDADILAAIEAAITDGVDVLSLSLGRGSMEFF 244
           GTAKGGSPKA VAAYKVCW     GG C DADILAA+EAAI+DGVDVLSLSLG GS +F 
Sbjct: 106 GTAKGGSPKALVAAYKVCWPQVDSGGGCYDADILAAMEAAISDGVDVLSLSLGGGSKDFS 165

Query: 245 DDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFTVAASTINRQFTSYVALGNE 304
           DDV AIGAFHAVQQG+VVVC+ GNSGP   +VENVAPW+ TV ASTINR FTS+V+LGN+
Sbjct: 166 DDVTAIGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIITVGASTINRDFTSFVSLGNK 225

Query: 305 KNISGSLDPRKV--KGKIIVCVRGDDARVN------------------------------ 364
           K+I G+    K+  + K    +   DA+ N                              
Sbjct: 226 KHIKGASLSDKILPEQKFYPLIDAVDAKANKVSSDIAQLCLVGSLDPEKVKGKIIICLRG 285

Query: 365 ------KGFVAAKAGAVGMILANNEEYGNDVVADAHILPVSHI----------------- 424
                 KG+ A KAGAVGMILAN EE G++++ADAH+LPVSH+                 
Sbjct: 286 ENARADKGYAAVKAGAVGMILANAEENGDEIIADAHLLPVSHVSYTDGQSIYQYINSTKT 345

Query: 425 ----------KMGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSKDASPSGS 484
                     ++GIKPAPVMASFSSRGP+++EE+ILKPDITAPG+NI+AAYS+DASPSGS
Sbjct: 346 PMAYMTHVRTELGIKPAPVMASFSSRGPNTVEETILKPDITAPGVNILAAYSEDASPSGS 405

Query: 485 PFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPIL 544
            FD RRIPFN+VSGTSMSCPHISGIV LLKT+YP WSPAAIKSAIMTTAE+RAND+HPI 
Sbjct: 406 FFDKRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAESRANDMHPIQ 465

Query: 545 DFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVC 604
           +   L AN  AYGAGHVQPNRA NPGLVYDLTT DY+NFLCA+GYNK+Q+SKFS  SFVC
Sbjct: 466 NGGNLKANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLCAQGYNKSQISKFSAMSFVC 525

Query: 605 SKSFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKF 646
           SKSFKLTDFNYPSISIP+MKSG VTIKR VKNVG PSTYVARV+VP GV VS++P TLKF
Sbjct: 526 SKSFKLTDFNYPSISIPDMKSGVVTIKRRVKNVGKPSTYVARVKVPHGVSVSVEPRTLKF 585

BLAST of CmaCh16G009850 vs. NCBI nr
Match: gi|778688353|ref|XP_011652727.1| (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus])

HSP 1 Score: 740.0 bits (1909), Expect = 3.6e-210
Identity = 387/606 (63.86%), Postives = 446/606 (73.60%), Query Frame = 1

Query: 105 ENGYEAIPSRWRGSCEGGSNFHCNKKLIGARYFNKGYVALAGSLDVSFDTARDQEGHGTH 164
           + GY  +P+RW+GSCEGGS FHCN+KLIGA+YFNKGY A AGSL+ S++TARD EGHGTH
Sbjct: 163 DEGYGPVPTRWKGSCEGGSKFHCNRKLIGAKYFNKGYAANAGSLNASYETARDNEGHGTH 222

Query: 165 TLSTAGGSFVSGANVFGYGNGTAKGGSPKARVAAYKVCWLTFRGGQCSDADILAAIEAAI 224
           TLSTAGG+F+SGAN                             GG C DADILAA+EAAI
Sbjct: 223 TLSTAGGNFISGANG-------------------------PLNGGGCYDADILAAMEAAI 282

Query: 225 TDGVDVLSLSLGRGSMEFFDDVIAIGAFHAVQQGMVVVCAGGNSGPDLQSVENVAPWLFT 284
           +DGVDVLSLSLG GS +F DDV AIGAFHAVQQG+VVVC+ GNSGP   +VENVAPW+ T
Sbjct: 283 SDGVDVLSLSLGGGSKDFSDDVTAIGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIIT 342

Query: 285 VAASTINRQFTSYVALGNEKNISGSLDPRKV--KGKIIVCVRGDDARVN----------- 344
           V ASTINR FTS+V+LGN+K+I G+    K+  + K    +   DA+ N           
Sbjct: 343 VGASTINRDFTSFVSLGNKKHIKGASLSDKILPEQKFYPLIDAVDAKANKVSSDIAQLCL 402

Query: 345 -------------------------KGFVAAKAGAVGMILANNEEYGNDVVADAHILPVS 404
                                    KG+ A KAGAVGMILAN EE G++++ADAH+LPVS
Sbjct: 403 VGSLDPEKVKGKIIICLRGENARADKGYAAVKAGAVGMILANAEENGDEIIADAHLLPVS 462

Query: 405 HI---------------------------KMGIKPAPVMASFSSRGPSSIEESILKPDIT 464
           H+                           ++GIKPAPVMASFSSRGP+++EE+ILKPDIT
Sbjct: 463 HVSYTDGQSIYQYINSTKTPMAYMTHVRTELGIKPAPVMASFSSRGPNTVEETILKPDIT 522

Query: 465 APGINIIAAYSKDASPSGSPFDNRRIPFNLVSGTSMSCPHISGIVALLKTIYPKWSPAAI 524
           APG+NI+AAYS+DASPSGS FD RRIPFN+VSGTSMSCPHISGIV LLKT+YP WSPAAI
Sbjct: 523 APGVNILAAYSEDASPSGSFFDKRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAI 582

Query: 525 KSAIMTTAETRANDLHPILDFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLC 584
           KSAIMTTAE+RAND+HPI +   L AN  AYGAGHVQPNRA NPGLVYDLTT DY+NFLC
Sbjct: 583 KSAIMTTAESRANDMHPIQNGGNLKANSFAYGAGHVQPNRAMNPGLVYDLTTKDYMNFLC 642

Query: 585 ARGYNKAQLSKFSNTSFVCSKSFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVA 644
           A+GYNK+Q+SKFS  SFVCSKSFKLTDFNYPSISIP+MKSG VTIKR VKNVG PSTYVA
Sbjct: 643 AQGYNKSQISKFSAMSFVCSKSFKLTDFNYPSISIPDMKSGVVTIKRRVKNVGKPSTYVA 702

Query: 645 RVEVPPGVLVSIKPSTLKFTRTDEEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSS 646
           RV+VP GV VS++P TLKFTR DEEK+F+VV  SVANNKH+GYVFGSL W DGKHHVRS 
Sbjct: 703 RVKVPHGVSVSVEPRTLKFTRIDEEKSFKVVIGSVANNKHKGYVFGSLIWEDGKHHVRSP 743

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT54_ARATH5.1e-18049.72Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1[more]
AIR3_ARATH1.4e-16951.48Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1[more]
SBT17_ARATH2.2e-13540.14Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1[more]
SBT11_ARATH2.8e-12238.86Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1[more]
SBT18_ARATH1.4e-12143.83Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2R9_CUCSA1.1e-22659.86Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1[more]
A0A0A0L613_CUCSA4.9e-21467.29Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045110 PE=4 SV=1[more]
A0A0D2U544_GOSRA1.3e-19351.84Uncharacterized protein OS=Gossypium raimondii GN=B456_009G453100 PE=4 SV=1[more]
A0A0A0L601_CUCSA1.0e-18756.53Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045060 PE=4 SV=1[more]
A0A059BWH5_EUCGR1.8e-18751.07Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03662 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59810.12.9e-18149.72 Subtilase family protein[more]
AT2G04160.17.8e-17151.48 Subtilisin-like serine endopeptidase family protein[more]
AT5G67360.11.3e-13640.14 Subtilase family protein[more]
AT1G01900.11.6e-12338.86 subtilase family protein[more]
AT2G05920.18.0e-12343.83 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|659096606|ref|XP_008449186.1|1.8e-23863.12PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103491136 [Cucumis me... [more]
gi|778675733|ref|XP_011650462.1|3.0e-22859.94PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus][more]
gi|700200865|gb|KGN55998.1|1.6e-22659.86hypothetical protein Csa_3G045120 [Cucumis sativus][more]
gi|700200864|gb|KGN55997.1|7.1e-21467.29hypothetical protein Csa_3G045110 [Cucumis sativus][more]
gi|778688353|ref|XP_011652727.1|3.6e-21063.86PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR003137PA_domain
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G009850.1CmaCh16G009850.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 123..298
score: 3.5E-66coord: 374..501
score: 3.5
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 123..470
score: 5.8
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 123..306
score: 3.53E-66coord: 378..505
score: 3.53
IPR003137PA domainPFAMPF02225PAcoord: 306..369
score: 5.
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9GENE3DG3DSA:3.30.70.80coord: 54..95
score: 1.
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 46..94
score: 1.
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 431..447
score: 4.2E-8coord: 157..170
score: 4.
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 45..643
score:
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 432..442
scor
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 299..353
score: 9.
NoneNo IPR availablePANTHERPTHR10795:SF3SUBFAMILY NOT NAMEDcoord: 45..643
score:
NoneNo IPR availableunknownSSF52025PA domaincoord: 312..374
score: 5.4

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh16G009850Cucsa.041770Cucumber (Gy14) v1cgycmaB0029
CmaCh16G009850Csa3G045110Cucumber (Chinese Long) v2cmacuB327
The following gene(s) are paralogous to this gene:

None