CmaCh16G009350 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G009350
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPectin lyase-like superfamily protein, putative
LocationCma_Chr16 : 7181945 .. 7183366 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATGTTTAGGGTTTTTTATCATCTTACGTTCCATTCCTAAAGGTAATTCCAATCTAAAGAGTTCAATATCATCCAATTTGGTGCAGTAATGGATGAAAAAACCGATGGTACGAAGGTGAAAGTTTTAAACTCAATACAAACAACCTTTTGTTATGTACTTACAATATTGTTCGATAACATTTTTTTTTTTTAAATAGAATCTTTAGAAGCATTATTTTAAAAATACCATTTTCGAAATGGAATACAATTTTTGGAAACATGACAAGCCTAGTTTTTTTTTAATATCTATTTCATGTCTTCAAATATCCAAATCAAATTTTTTAAACTAAAAAAAATATTTTTTTGTTGTTTTTGGCTTATTCTTCCTAATAAATATCTAAAAGTAAATTTAACATCAAAATTGTGCAAATTTAATTTGAAACTACCTTTGGTATTACTTTATTGTAAATTAAGAAGTACATTAAAAAATTTAAGCTATTTGGTTGAAAGTAGATAGATAAAAGATTACTTGGTTTGTATAGGCATTATGAGGCATGGAGAGAAGCTTGTGGACATAACGGACATGGTGTAGTTTTAGTACCAGCGGAGATTCTTGGTTTTGCCGTGGGTGCTTGAAGGTCCATGCAAAGTATCCATTGGTCTACTTATTCATGGTGAACTGTTGGCACCTGTCAGTAAGAATTTCGCCACTGGTGTATACTGGCTGAGTATCTCCAAAGTCGACAACTTGTTCGTTGACGGTTCTGGCAGCTTGAATGGCGGCGGTTCTTCAGCTTGGCCTTCCGACACTTCTAACCGCCCAGTTGTAAGTCCATTTTTAGGTTTGAGTGATAAAAAAGAAATTCATTTTGTTAGGTTGATAGTTAAATGATGCTATCATTTTACATTTAAAAGTACACAAAAATATACATATGTATAATCTAACGATTGATTCAATGTAGTCGATGAAACTTTCTAGTGTCAACACTGCGAGAATTTCAAAGATAACATCGTCCAATAGAAAACTCTTCCATTTTGGTGTGCATGGATGTCATGATATCACATTTGATCACGTTACAGTGATAGCTCCAGCAGATAGCCCCAATACAGATGAAATTCACGTTTTCAAGTCAAGTGGTATCAATATTATGCATTCAGTGATTCGCAGTGGAGACGATTGCATCTCTTTAGGCCCTGGAAGTAAATTCATCAACATAACAAATGTCCAGTGTGGTCCAGGTCATGGAATTAGTATCGGTAGCCTCGGAAAGTACCCTAATGAAGAAGATGTCTCTGAAGTCACAATAAGAGACTCCACACTTGTTGGCACAGACAATGGTGTGAGGATCAAAACATGGTCATTTTCATTTTCTAATTTTGTTAGCGATCGCTTTTCTCAATCTCGAAATGAAGGATGTAAAAAATCCTATCATCATTGA

mRNA sequence

ATGAGATGTTTAGGGTTTTTTATCATCTTACGTTCCATTCCTAAAGGTCCATGCAAAGTATCCATTGGTCTACTTATTCATGGTGAACTGTTGGCACCTGTCAGTAAGAATTTCGCCACTGGTGTATACTGGCTGAGTATCTCCAAAGTCGACAACTTGTTCGTTGACGGTTCTGGCAGCTTGAATGGCGGCGGTTCTTCAGCTTGGCCTTCCGACACTTCTAACCGCCCAGTTTCGATGAAACTTTCTAGTGTCAACACTGCGAGAATTTCAAAGATAACATCGTCCAATAGAAAACTCTTCCATTTTGGTGTGCATGGATGTCATGATATCACATTTGATCACGTTACAGTGATAGCTCCAGCAGATAGCCCCAATACAGATGAAATTCACGTTTTCAAGTCAAGTGGTATCAATATTATGCATTCAGTGATTCGCAGTGGAGACGATTGCATCTCTTTAGGCCCTGGAAGTAAATTCATCAACATAACAAATGTCCAGTGTGGTCCAGGTCATGGAATTAGTATCGGTAGCCTCGGAAAGTACCCTAATGAAGAAGATGTCTCTGAAGTCACAATAAGAGACTCCACACTTGTTGGCACAGACAATGGTGTGAGGATCAAAACATGGTCATTTTCATTTTCTAATTTTGTTAGCGATCGCTTTTCTCAATCTCGAAATGAAGGATGTAAAAAATCCTATCATCATTGA

Coding sequence (CDS)

ATGAGATGTTTAGGGTTTTTTATCATCTTACGTTCCATTCCTAAAGGTCCATGCAAAGTATCCATTGGTCTACTTATTCATGGTGAACTGTTGGCACCTGTCAGTAAGAATTTCGCCACTGGTGTATACTGGCTGAGTATCTCCAAAGTCGACAACTTGTTCGTTGACGGTTCTGGCAGCTTGAATGGCGGCGGTTCTTCAGCTTGGCCTTCCGACACTTCTAACCGCCCAGTTTCGATGAAACTTTCTAGTGTCAACACTGCGAGAATTTCAAAGATAACATCGTCCAATAGAAAACTCTTCCATTTTGGTGTGCATGGATGTCATGATATCACATTTGATCACGTTACAGTGATAGCTCCAGCAGATAGCCCCAATACAGATGAAATTCACGTTTTCAAGTCAAGTGGTATCAATATTATGCATTCAGTGATTCGCAGTGGAGACGATTGCATCTCTTTAGGCCCTGGAAGTAAATTCATCAACATAACAAATGTCCAGTGTGGTCCAGGTCATGGAATTAGTATCGGTAGCCTCGGAAAGTACCCTAATGAAGAAGATGTCTCTGAAGTCACAATAAGAGACTCCACACTTGTTGGCACAGACAATGGTGTGAGGATCAAAACATGGTCATTTTCATTTTCTAATTTTGTTAGCGATCGCTTTTCTCAATCTCGAAATGAAGGATGTAAAAAATCCTATCATCATTGA

Protein sequence

MRCLGFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGGGSSAWPSDTSNRPVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSPNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDVSEVTIRDSTLVGTDNGVRIKTWSFSFSNFVSDRFSQSRNEGCKKSYHH
BLAST of CmaCh16G009350 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.5e-47
Identity = 92/204 (45.10%), Postives = 135/204 (66.18%), Query Frame = 1

Query: 15  KGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGGGSSAWPSDT- 74
           +GPCK SIGL + G L AP   +   G+ W++++K+D L + G G  +G G SAW  +  
Sbjct: 37  EGPCKSSIGLQLQGTLKAPADPSKIKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDC 96

Query: 75  -SNRPV------SMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSPN 134
             N P+      +++L +V  + +  +T+ + K FH  V GC ++TF+   + A   S N
Sbjct: 97  HKNGPICKTLSMNLRLYAVTNSILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSIN 156

Query: 135 TDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEE 194
           TD IH+ +S G+NI+++ I++GDDCISLG GSK INITN+ CGPGHGIS+GSLG+Y NEE
Sbjct: 157 TDGIHIGRSDGVNIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEE 216

Query: 195 DVSEVTIRDSTLVGTDNGVRIKTW 211
            V  + +++ T+ G+ NGVRIKTW
Sbjct: 217 SVVGIYVKNCTITGSQNGVRIKTW 240

BLAST of CmaCh16G009350 vs. Swiss-Prot
Match: PGLR2_PLAAC (Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 7.1e-47
Identity = 97/204 (47.55%), Postives = 130/204 (63.73%), Query Frame = 1

Query: 15  KGPCKVS-IGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGGGSSAWPSDT 74
           +GPCK S IG  I G + AP   +      W+S  ++D L V G+G+L+G G +AW  + 
Sbjct: 58  QGPCKGSKIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNN 117

Query: 75  SNRPVSMKLSSVNT-------ARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSPN 134
            ++  + K +++N        A +  ITS N K+FH  V  C DITF HVTV AP  S N
Sbjct: 118 CDKNPNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSIN 177

Query: 135 TDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEE 194
           TD IHV  S G+ I ++ I +GDDCIS+GPGS+ + IT V CGPGHGISIGSLG+Y NE+
Sbjct: 178 TDGIHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEK 237

Query: 195 DVSEVTIRDSTLVGTDNGVRIKTW 211
           +V  +T++  T  GT NGVR+KTW
Sbjct: 238 EVRGITVKGCTFSGTMNGVRVKTW 261

BLAST of CmaCh16G009350 vs. Swiss-Prot
Match: PGLR3_MAIZE (Exopolygalacturonase OS=Zea mays GN=PG2C PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.9e-45
Identity = 91/203 (44.83%), Postives = 126/203 (62.07%), Query Frame = 1

Query: 16  GPCKVSIGLLIHGELLAPVS-KNFATGVYWLSISKVDNLFVDGSGSLNGGGSSAWPSDTS 75
           GPCK  + + ++G LLA      +     W+ I +VDNL + G G L+G G + W  ++ 
Sbjct: 92  GPCKGDVTIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSC 151

Query: 76  NR-------PVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSPNT 135
            +       P S+ +  VN   +S IT  N K FH  ++ C D+    V V AP DSPNT
Sbjct: 152 VKKYDCKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNT 211

Query: 136 DEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEED 195
           D IH+  SSG+ I ++VI  GDDCIS+GPG+  +NIT V CGPGHGISIGSLG+Y +E+D
Sbjct: 212 DGIHMGDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKD 271

Query: 196 VSEVTIRDSTLVGTDNGVRIKTW 211
           V+++ ++D TL  T NGVRIK +
Sbjct: 272 VTDINVKDCTLKKTANGVRIKAY 294

BLAST of CmaCh16G009350 vs. Swiss-Prot
Match: PGLR1_MAIZE (Exopolygalacturonase OS=Zea mays GN=PG1 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 6.7e-45
Identity = 93/214 (43.46%), Postives = 131/214 (61.21%), Query Frame = 1

Query: 5   GFFIILRSIPKGPCKVSIGLLIHGELLAPVS-KNFATGVYWLSISKVDNLFVDGSGSLNG 64
           G F++ +    GPCK  + + + G LLA      +     W+ I +VDNL + G G+L+G
Sbjct: 81  GDFLVGQLNFTGPCKGDVTIQVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDG 140

Query: 65  GGSSAWPSDTSNR-------PVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHV 124
            G + W  ++  +       P S+ +  VN   +S +T  N K FH  ++ C D+    V
Sbjct: 141 QGPAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDV 200

Query: 125 TVIAPADSPNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISI 184
           TV AP DSPNTD IH+  SSGI I ++VI  GDDCIS+GPG+  +NIT V CGPGHGISI
Sbjct: 201 TVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISI 260

Query: 185 GSLGKYPNEEDVSEVTIRDSTLVGTDNGVRIKTW 211
           GSLG+Y +E+DV+++ ++D TL  T  GVRIK +
Sbjct: 261 GSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAY 294

BLAST of CmaCh16G009350 vs. Swiss-Prot
Match: PGLR_MEDSA (Polygalacturonase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 6.7e-45
Identity = 93/208 (44.71%), Postives = 126/208 (60.58%), Query Frame = 1

Query: 15  KGPCKVSIGLLIHGELLAPVSKNFATGV-YWLSISKVDNLFVDGSGSLNGGGS------- 74
           KGPCK  I L + G + AP   +   G   W     +D+L + G G  +G G+       
Sbjct: 73  KGPCKAPIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKKAA 132

Query: 75  --SAWPSDTSNRPVSMKL--SSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPA 134
             SAW    SN  V M    + VN + +  +TS + K FH  V GC +ITFD  T+ AP 
Sbjct: 133 PASAWSGKNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPG 192

Query: 135 DSPNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKY 194
           DSPNTD IH+ KS+ + I+++ I +GDDC+S+G GSK I +  V CGPGHG+S+GSLGK+
Sbjct: 193 DSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKF 252

Query: 195 PNEEDVSEVTIRDSTLVGTDNGVRIKTW 211
             EE+V  +T+++ TL  TDNGVRIKTW
Sbjct: 253 TTEENVEGITVKNCTLTATDNGVRIKTW 280

BLAST of CmaCh16G009350 vs. TrEMBL
Match: A0A0A0L7H9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G063610 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 1.7e-84
Identity = 151/215 (70.23%), Postives = 180/215 (83.72%), Query Frame = 1

Query: 5   GFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGG 64
           G F++L  + +GPC   I + + GELLAP+ ++FATG YWLSI +V+NLF+DG GSL+G 
Sbjct: 39  GRFLVLPLLLQGPCHGFIRIQLDGELLAPLDEHFATGDYWLSIDQVNNLFIDGLGSLDGR 98

Query: 65  GSSAWPSDTSNRPVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADS 124
           GS+AWPS + NRPVSM+L+ +N ARI+ I S N KLFHF +HGC D+TFDHVTVIAPA+S
Sbjct: 99  GSTAWPSTSHNRPVSMRLNGINNARITNIKSYNSKLFHFAMHGCRDVTFDHVTVIAPANS 158

Query: 125 PNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPN 184
           PNTD IH+  SSGINIMHS I +GDDCISLGPGSKFINITN+QCGPGHGISIGSLGKYPN
Sbjct: 159 PNTDGIHISSSSGINIMHSTIGTGDDCISLGPGSKFINITNIQCGPGHGISIGSLGKYPN 218

Query: 185 EEDVSEVTIRDSTLVGTDNGVRIKTWSFSFSNFVS 220
           EEDV EVT+RDST +GT NGVRIKTWS S+S+ VS
Sbjct: 219 EEDVFEVTVRDSTFIGTSNGVRIKTWSSSYSSMVS 253

BLAST of CmaCh16G009350 vs. TrEMBL
Match: A0A061F588_THECC (Pectin lyase-like superfamily protein, putative OS=Theobroma cacao GN=TCM_030754 PE=3 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 4.3e-51
Identity = 108/224 (48.21%), Postives = 145/224 (64.73%), Query Frame = 1

Query: 4   LGFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNG 63
           LG +++L ++ +GPCK +I   + G L AP         +W+S   +D L + G GSL+G
Sbjct: 73  LGTYLVLPTVFQGPCKGAIAFQVKGLLRAPTDAASLHLDHWISFRYIDRLTITGGGSLDG 132

Query: 64  GGSSAWPSDTSNR-------PVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHV 123
            G+SAWP +T          PV+++   V  + I+ ITS N K FHF +    +I    V
Sbjct: 133 QGASAWPYNTCLTDPNCPPFPVTLRFDFVTNSWINHITSINSKNFHFNIFASTNIRIHQV 192

Query: 124 TVIAPADSPNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISI 183
            + AP DSPNTD IH+  S+GI+I  S I +GDDC+S+GPGS+ INITNV CGPGHG S+
Sbjct: 193 NISAPGDSPNTDGIHIGDSTGIHISDSEIATGDDCVSMGPGSQNINITNVHCGPGHGFSV 252

Query: 184 GSLGKYPNEEDVSEVTIRDSTLVGTDNGVRIKTWSFSFSNFVSD 221
           GSLGK PNE+DV+ VT+R+ TL GT NG+RIKTWS S S+  SD
Sbjct: 253 GSLGKSPNEKDVAGVTVRNCTLTGTLNGLRIKTWSPSHSSRCSD 296

BLAST of CmaCh16G009350 vs. TrEMBL
Match: A0A0A0L660_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G063620 PE=3 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 2.8e-50
Identity = 97/140 (69.29%), Postives = 114/140 (81.43%), Query Frame = 1

Query: 80  MKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSPNTDEIHVFKSSGIN 139
           MK ++VNTA I  I+S + K FHF VH   D+TFD+VTV+APA+SPNTD IH+  SSGIN
Sbjct: 1   MKFNNVNTASIKNISSFDSKFFHFAVHASRDVTFDNVTVVAPANSPNTDGIHISTSSGIN 60

Query: 140 IMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDVSEVTIRDSTLV 199
           IMHS I +GDDCISLGPGSK INI+NV CGPGHGISIGSLGK PNEEDV +VT+RDST +
Sbjct: 61  IMHSTIGTGDDCISLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFI 120

Query: 200 GTDNGVRIKTWSFSFSNFVS 220
           GT NG RIK+WS  +S+ VS
Sbjct: 121 GTSNGARIKSWSSPYSSMVS 140

BLAST of CmaCh16G009350 vs. TrEMBL
Match: A0A059CRS9_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C02249 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 3.7e-50
Identity = 99/215 (46.05%), Postives = 137/215 (63.72%), Query Frame = 1

Query: 4   LGFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNG 63
           LG +++   + KGPC   I + + G +  PV K+  +  +W++   VD L +DG G+ +G
Sbjct: 79  LGTYMVWPVVLKGPCAGPITVTVEGVVKGPVDKSTFSLDHWITFQSVDGLLIDGGGTFDG 138

Query: 64  GGSSAWPSDTSNR-------PVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHV 123
            G  AW  +  ++       PVS++ + V  A IS ITS N   FHF +  C  + F HV
Sbjct: 139 QGQEAWSYNNCSKNSNCKLLPVSLRFNYVKNALISNITSVNSMFFHFVLFSCDALEFRHV 198

Query: 124 TVIAPADSPNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISI 183
            ++AP +SPNTD IH+  SS I I  SVI +GDDC+S+GPGSK + +  VQCGPGHG S+
Sbjct: 199 RIVAPDESPNTDGIHIGHSSRITIADSVISTGDDCVSVGPGSKGVKVVGVQCGPGHGFSV 258

Query: 184 GSLGKYPNEEDVSEVTIRDSTLVGTDNGVRIKTWS 212
           GSLGKYP E DVSE+++   T+VGT NGVRIKTW+
Sbjct: 259 GSLGKYPGEADVSELSVSGCTMVGTQNGVRIKTWA 293

BLAST of CmaCh16G009350 vs. TrEMBL
Match: W9RBH8_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_014009 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.4e-49
Identity = 100/222 (45.05%), Postives = 141/222 (63.51%), Query Frame = 1

Query: 5   GFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGV-YWLSISKVDNLFVDGSGSLNG 64
           G +++   + KG CK  +   + G+L AP  +     + +W++   VDN  + G GSL+G
Sbjct: 69  GTYLVNPVVLKGTCKGQMVFFLQGDLKAPTDQKSWDDIDHWITFQYVDNFVMSGGGSLDG 128

Query: 65  GGSSAWPSDTSNR-------PVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHV 124
            G+SAWP +T N+       PVS++L  VN A IS I   N K FH  +  C++I  +H+
Sbjct: 129 QGASAWPYNTCNKDSHCKKLPVSLRLDFVNNAWISYIKLINSKNFHMNIFACNNIKLEHM 188

Query: 125 TVIAPADSPNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISI 184
            ++AP DSPNTD IH+  S+GI +  S+I +GDDC+S GPG K ++I  VQCGPGHGISI
Sbjct: 189 HIMAPGDSPNTDGIHIALSTGIKVWDSIISTGDDCLSFGPGMKDVDIWKVQCGPGHGISI 248

Query: 185 GSLGKYPNEEDVSEVTIRDSTLVGTDNGVRIKTWSFSFSNFV 219
           GSLG  PNE+DV    +R+S  +GT NGVRIKTW+   ++ V
Sbjct: 249 GSLGSSPNEKDVQGFIVRNSRFIGTQNGVRIKTWALPHASNV 290

BLAST of CmaCh16G009350 vs. TAIR10
Match: AT3G07840.1 (AT3G07840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 178.3 bits (451), Expect = 5.4e-45
Identity = 88/202 (43.56%), Postives = 131/202 (64.85%), Query Frame = 1

Query: 16  GPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGGGSSAWPSDTSN 75
           GPCK  + + + G L A   K       W+   +++   ++G G  +G G++AW  +  +
Sbjct: 72  GPCKAPVEINLQGTLKAD-GKAIQGKERWVVFLRINGFKLNGGGIFDGEGNAAWRVNNCH 131

Query: 76  R-------PVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSPNTD 135
           +       P+S++   V  A I  I+S + K FH  V G  ++TFD+V VIAPA+SPNTD
Sbjct: 132 KTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAPAESPNTD 191

Query: 136 EIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDV 195
            IH+ +S G+ I++S I +GDDCIS+G G K +++ NV CGPGHGIS+GSLG+Y +E+DV
Sbjct: 192 GIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGRYVHEQDV 251

Query: 196 SEVTIRDSTLVGTDNGVRIKTW 211
           + +T+ + TL GTDNG+RIKTW
Sbjct: 252 TGITVVNCTLQGTDNGLRIKTW 272

BLAST of CmaCh16G009350 vs. TAIR10
Match: AT2G26620.1 (AT2G26620.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 174.9 bits (442), Expect = 6.0e-44
Identity = 91/214 (42.52%), Postives = 127/214 (59.35%), Query Frame = 1

Query: 16  GPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGGGSSAWP-SDTS 75
           GPCK  I  +I G LLAP + N      W++   ++NL + GSG+L+G G  +WP +D  
Sbjct: 83  GPCKNPIEFIIDGTLLAPANPNDIKQDTWINFRYINNLSISGSGTLDGQGKQSWPLNDCH 142

Query: 76  NRP------VSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSPNTD 135
             P      +SM  + VN + I  ITS N K+ HF     H      VT+ AP+DSPNTD
Sbjct: 143 KNPNCPKLAISMGFAFVNNSNIKDITSLNSKMGHFNFFFVHHFNITGVTITAPSDSPNTD 202

Query: 136 EIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDV 195
            I +   S I I ++ I +GDDCI++  G+  +NI+N+ CGPGHGIS+GSLGK  +E+DV
Sbjct: 203 GIKMGSCSNIQISNTNIGTGDDCIAILSGTTKLNISNINCGPGHGISVGSLGKNKDEKDV 262

Query: 196 SEVTIRDSTLVGTDNGVRIKTWSFSFSNFVSDRF 223
            ++ +RD    GT +G+RIKTW  S S  +   F
Sbjct: 263 KDLFVRDVIFNGTSDGIRIKTWESSASKILVSNF 296

BLAST of CmaCh16G009350 vs. TAIR10
Match: AT1G43090.1 (AT1G43090.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 174.9 bits (442), Expect = 6.0e-44
Identity = 90/214 (42.06%), Postives = 129/214 (60.28%), Query Frame = 1

Query: 16  GPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGGGSSAWPSDTSN 75
           GPCK  I  +I G LLAP +        W++   ++NL + GSG+L+G G  +WP +  +
Sbjct: 83  GPCKNPIEFVIDGTLLAPANPRDIKQDTWINFRYINNLSISGSGTLDGQGKYSWPLNDCH 142

Query: 76  R-------PVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSPNTD 135
           +        ++M  + VN +RI  ITS N K+ HF     H      VT+ AP DSPNTD
Sbjct: 143 KNTNCPKLAMTMGFAFVNNSRIKDITSLNSKMGHFNFFSVHRFNITGVTITAPGDSPNTD 202

Query: 136 EIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDV 195
            I +   S I+I ++ I +GDDCI++  G+  ++I+NV+CGPGHGIS+GSLGK  +E+DV
Sbjct: 203 GIKMGSCSNIHISNTNIGTGDDCIAILSGTTNLDISNVKCGPGHGISVGSLGKNKDEKDV 262

Query: 196 SEVTIRDSTLVGTDNGVRIKTWSFSFSNFVSDRF 223
             +T+RD+   GT +G+RIKTW  S S  V   F
Sbjct: 263 KHLTVRDTVFNGTSDGIRIKTWESSASKLVVSNF 296

BLAST of CmaCh16G009350 vs. TAIR10
Match: AT1G43080.1 (AT1G43080.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 174.5 bits (441), Expect = 7.8e-44
Identity = 89/214 (41.59%), Postives = 129/214 (60.28%), Query Frame = 1

Query: 16  GPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGGGSSAWPSDTSN 75
           GPCK  I  +I G LLAP +        W++   ++NL + GSG+L+G G  +WP +  +
Sbjct: 83  GPCKNPIEFVIDGTLLAPANPRDIKQDTWINFRYINNLSISGSGTLDGQGKYSWPLNDCH 142

Query: 76  R-------PVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSPNTD 135
           +        ++M  + VN +RI  ITS N K+ HF     H      VT+ AP DSPNTD
Sbjct: 143 KNTNCPKLAMTMGFAFVNNSRIKDITSLNSKMGHFNFFSVHRFNITGVTITAPGDSPNTD 202

Query: 136 EIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNEEDV 195
            I +   S I+I ++ I +GDDCI++  G+  ++I+N++CGPGHGIS+GSLGK  +E+DV
Sbjct: 203 GIKMGSCSNIHISNTNIGTGDDCIAILSGTTNLDISNIKCGPGHGISVGSLGKNKDEKDV 262

Query: 196 SEVTIRDSTLVGTDNGVRIKTWSFSFSNFVSDRF 223
             +T+RD+   GT +G+RIKTW  S S  V   F
Sbjct: 263 KHLTVRDTVFNGTSDGIRIKTWESSASKIVVSNF 296

BLAST of CmaCh16G009350 vs. TAIR10
Match: AT3G07850.1 (AT3G07850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 174.1 bits (440), Expect = 1.0e-43
Identity = 82/205 (40.00%), Postives = 134/205 (65.37%), Query Frame = 1

Query: 15  KGPCKVSIGLLIHGELLAPVS-KNFATGVYWLSISKVDNLFVDGSGSL-NGGGSSAWPSD 74
           KGPCK  + L ++G   AP + K       W+    + +  ++G+ ++ +G GS AW ++
Sbjct: 118 KGPCKGPVTLELNGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKAN 177

Query: 75  T-------SNRPVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADSP 134
                   ++ P++++ + +  ++I+ ITS+N KLFH  +  C +IT   + + AP +S 
Sbjct: 178 DCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESL 237

Query: 135 NTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPNE 194
           NTD IH+ +S+G+N++ + I++GDDC+S+G G++ + + NV+CGPGHGISIGSLG+YPNE
Sbjct: 238 NTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNE 297

Query: 195 EDVSEVTIRDSTLVGTDNGVRIKTW 211
           + V  VT+R   +  TDNGVRIKTW
Sbjct: 298 QPVKGVTVRKCLIKNTDNGVRIKTW 322

BLAST of CmaCh16G009350 vs. NCBI nr
Match: gi|700200928|gb|KGN56061.1| (hypothetical protein Csa_3G063610 [Cucumis sativus])

HSP 1 Score: 320.5 bits (820), Expect = 2.5e-84
Identity = 151/215 (70.23%), Postives = 180/215 (83.72%), Query Frame = 1

Query: 5   GFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGG 64
           G F++L  + +GPC   I + + GELLAP+ ++FATG YWLSI +V+NLF+DG GSL+G 
Sbjct: 39  GRFLVLPLLLQGPCHGFIRIQLDGELLAPLDEHFATGDYWLSIDQVNNLFIDGLGSLDGR 98

Query: 65  GSSAWPSDTSNRPVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADS 124
           GS+AWPS + NRPVSM+L+ +N ARI+ I S N KLFHF +HGC D+TFDHVTVIAPA+S
Sbjct: 99  GSTAWPSTSHNRPVSMRLNGINNARITNIKSYNSKLFHFAMHGCRDVTFDHVTVIAPANS 158

Query: 125 PNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPN 184
           PNTD IH+  SSGINIMHS I +GDDCISLGPGSKFINITN+QCGPGHGISIGSLGKYPN
Sbjct: 159 PNTDGIHISSSSGINIMHSTIGTGDDCISLGPGSKFINITNIQCGPGHGISIGSLGKYPN 218

Query: 185 EEDVSEVTIRDSTLVGTDNGVRIKTWSFSFSNFVS 220
           EEDV EVT+RDST +GT NGVRIKTWS S+S+ VS
Sbjct: 219 EEDVFEVTVRDSTFIGTSNGVRIKTWSSSYSSMVS 253

BLAST of CmaCh16G009350 vs. NCBI nr
Match: gi|778688362|ref|XP_011652730.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 320.5 bits (820), Expect = 2.5e-84
Identity = 151/215 (70.23%), Postives = 180/215 (83.72%), Query Frame = 1

Query: 5   GFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGG 64
           G F++L  + +GPC   I + + GELLAP+ ++FATG YWLSI +V+NLF+DG GSL+G 
Sbjct: 39  GRFLVLPLLLQGPCHGFIRIQLDGELLAPLDEHFATGDYWLSIDQVNNLFIDGLGSLDGR 98

Query: 65  GSSAWPSDTSNRPVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADS 124
           GS+AWPS + NRPVSM+L+ +N ARI+ I S N KLFHF +HGC D+TFDHVTVIAPA+S
Sbjct: 99  GSTAWPSTSHNRPVSMRLNGINNARITNIKSYNSKLFHFAMHGCRDVTFDHVTVIAPANS 158

Query: 125 PNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPN 184
           PNTD IH+  SSGINIMHS I +GDDCISLGPGSKFINITN+QCGPGHGISIGSLGKYPN
Sbjct: 159 PNTDGIHISSSSGINIMHSTIGTGDDCISLGPGSKFINITNIQCGPGHGISIGSLGKYPN 218

Query: 185 EEDVSEVTIRDSTLVGTDNGVRIKTWSFSFSNFVS 220
           EEDV EVT+RDST +GT NGVRIKTWS S+S+ VS
Sbjct: 219 EEDVFEVTVRDSTFIGTSNGVRIKTWSSSYSSMVS 253

BLAST of CmaCh16G009350 vs. NCBI nr
Match: gi|659096624|ref|XP_008449197.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 318.9 bits (816), Expect = 7.3e-84
Identity = 156/216 (72.22%), Postives = 178/216 (82.41%), Query Frame = 1

Query: 4   LGFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNG 63
           LG F++L  + +GPC   I + + GELLAP+++N  TG YWLSI KV+NLF+DG G LNG
Sbjct: 38  LGRFLVLPLLLQGPCHGFIHIQLDGELLAPLNEN--TGDYWLSIDKVNNLFIDGLGRLNG 97

Query: 64  GGSSAWPSDTSNRPVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPAD 123
            GS+AWPS + NRP+SMKL+ +N ARIS I S N KLFHF VHGC D+TFDHVTVIAPA+
Sbjct: 98  RGSTAWPSASHNRPISMKLTGINNARISNIKSYNSKLFHFAVHGCRDVTFDHVTVIAPAN 157

Query: 124 SPNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYP 183
           SPNTD IHV  SSGINIMHS I +GDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYP
Sbjct: 158 SPNTDGIHVSLSSGINIMHSTIGTGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYP 217

Query: 184 NEEDVSEVTIRDSTLVGTDNGVRIKTWSFSFSNFVS 220
           NEEDV EVT+RDST +GT NG RIKTWS S+SN VS
Sbjct: 218 NEEDVFEVTVRDSTFLGTTNGARIKTWSSSYSNMVS 251

BLAST of CmaCh16G009350 vs. NCBI nr
Match: gi|449463753|ref|XP_004149596.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 284.6 bits (727), Expect = 1.5e-73
Identity = 136/215 (63.26%), Postives = 166/215 (77.21%), Query Frame = 1

Query: 5   GFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNGG 64
           G F++L  + +GPC   I + + GELLA   K FA G +WLSI  V+NLF+DG G L G 
Sbjct: 29  GKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIGDHWLSIENVNNLFIDGLGRLTGS 88

Query: 65  GSSAWPSDTSNRPVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHVTVIAPADS 124
           G+SAWPS++ NRP+SMK ++VNTA I  I+S + K FHF VH   D+TFD+VTV+APA+S
Sbjct: 89  GASAWPSNSLNRPISMKFNNVNTASIKNISSFDSKFFHFAVHASRDVTFDNVTVVAPANS 148

Query: 125 PNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISIGSLGKYPN 184
           PNTD IH+  SSGINIMHS I +GDDCISLGPGSK INI+NV CGPGHGISIGSLGK PN
Sbjct: 149 PNTDGIHISTSSGINIMHSTIGTGDDCISLGPGSKMINISNVHCGPGHGISIGSLGKNPN 208

Query: 185 EEDVSEVTIRDSTLVGTDNGVRIKTWSFSFSNFVS 220
           EEDV +VT+RDST +GT NG RIK+WS  +S+ VS
Sbjct: 209 EEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMVS 243

BLAST of CmaCh16G009350 vs. NCBI nr
Match: gi|590606088|ref|XP_007020651.1| (Pectin lyase-like superfamily protein, putative [Theobroma cacao])

HSP 1 Score: 209.5 bits (532), Expect = 6.2e-51
Identity = 108/224 (48.21%), Postives = 145/224 (64.73%), Query Frame = 1

Query: 4   LGFFIILRSIPKGPCKVSIGLLIHGELLAPVSKNFATGVYWLSISKVDNLFVDGSGSLNG 63
           LG +++L ++ +GPCK +I   + G L AP         +W+S   +D L + G GSL+G
Sbjct: 73  LGTYLVLPTVFQGPCKGAIAFQVKGLLRAPTDAASLHLDHWISFRYIDRLTITGGGSLDG 132

Query: 64  GGSSAWPSDTSNR-------PVSMKLSSVNTARISKITSSNRKLFHFGVHGCHDITFDHV 123
            G+SAWP +T          PV+++   V  + I+ ITS N K FHF +    +I    V
Sbjct: 133 QGASAWPYNTCLTDPNCPPFPVTLRFDFVTNSWINHITSINSKNFHFNIFASTNIRIHQV 192

Query: 124 TVIAPADSPNTDEIHVFKSSGINIMHSVIRSGDDCISLGPGSKFINITNVQCGPGHGISI 183
            + AP DSPNTD IH+  S+GI+I  S I +GDDC+S+GPGS+ INITNV CGPGHG S+
Sbjct: 193 NISAPGDSPNTDGIHIGDSTGIHISDSEIATGDDCVSMGPGSQNINITNVHCGPGHGFSV 252

Query: 184 GSLGKYPNEEDVSEVTIRDSTLVGTDNGVRIKTWSFSFSNFVSD 221
           GSLGK PNE+DV+ VT+R+ TL GT NG+RIKTWS S S+  SD
Sbjct: 253 GSLGKSPNEKDVAGVTVRNCTLTGTLNGLRIKTWSPSHSSRCSD 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR_OENOR2.5e-4745.10Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1[more]
PGLR2_PLAAC7.1e-4747.55Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1[more]
PGLR3_MAIZE3.9e-4544.83Exopolygalacturonase OS=Zea mays GN=PG2C PE=2 SV=1[more]
PGLR1_MAIZE6.7e-4543.46Exopolygalacturonase OS=Zea mays GN=PG1 PE=1 SV=1[more]
PGLR_MEDSA6.7e-4544.71Polygalacturonase OS=Medicago sativa PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L7H9_CUCSA1.7e-8470.23Uncharacterized protein OS=Cucumis sativus GN=Csa_3G063610 PE=3 SV=1[more]
A0A061F588_THECC4.3e-5148.21Pectin lyase-like superfamily protein, putative OS=Theobroma cacao GN=TCM_030754... [more]
A0A0A0L660_CUCSA2.8e-5069.29Uncharacterized protein OS=Cucumis sativus GN=Csa_3G063620 PE=3 SV=1[more]
A0A059CRS9_EUCGR3.7e-5046.05Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C02249 PE=3 SV=1[more]
W9RBH8_9ROSA2.4e-4945.05Uncharacterized protein OS=Morus notabilis GN=L484_014009 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G07840.15.4e-4543.56 Pectin lyase-like superfamily protein[more]
AT2G26620.16.0e-4442.52 Pectin lyase-like superfamily protein[more]
AT1G43090.16.0e-4442.06 Pectin lyase-like superfamily protein[more]
AT1G43080.17.8e-4441.59 Pectin lyase-like superfamily protein[more]
AT3G07850.11.0e-4340.00 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|700200928|gb|KGN56061.1|2.5e-8470.23hypothetical protein Csa_3G063610 [Cucumis sativus][more]
gi|778688362|ref|XP_011652730.1|2.5e-8470.23PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
gi|659096624|ref|XP_008449197.1|7.3e-8472.22PREDICTED: exopolygalacturonase-like [Cucumis melo][more]
gi|449463753|ref|XP_004149596.1|1.5e-7363.26PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
gi|590606088|ref|XP_007020651.1|6.2e-5148.21Pectin lyase-like superfamily protein, putative [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G009350.1CmaCh16G009350.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 15..214
score: 7.3
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 165..178
scor
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 15..220
score: 4.56
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 10..211
score: 6.9
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 5..210
score: 7.8

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh16G009350MELO3C014147Melon (DHL92) v3.5.1cmameB290
The following gene(s) are paralogous to this gene:

None