CmaCh16G009180 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G009180
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCytochrome P450 family protein
LocationCma_Chr16 : 6924709 .. 6927354 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATATCAAAAAGTTGCCTCCTGGTCCAAAAGGATTCCCCATTATTGGTACCATCCATCTATTGAAAAAGCTTGTTCATAGAGATCTTCATCACTTATCTCAACTCTACGGACCCATAATGCACATGAAATTGGGACTCAAATCCACTATTATTGTCTCTTCCTCTCATGCTGCTGAGCTCTTCCTCAAAGCCCATGACCCAGTTTTTGCAGGCCGCCCAGTTCGTTTAACCTTCAACCAACTGAGTTACCACCAAAAAAACATAGCCTTTGCTCAATATGGCCCTTATTGGCGCAATATGCGTAAAATATGCATACTTCAATTGTTTACAACTCTCAAAATGAACTCCTTTACGTCAATGAGAAGAGAAGAGGTTGGTTTGTTGATTGATCATCTTCAAGAGGCTGCAAAAATGCATGGTGTTGTGGATCTCAGCTCTCAAATCTCGTCTTTTATTGCTGATGTGATTTGTAGAATGGTGTTTGGGAGAAAATTTGTAGAGGAAGAATTTAAGGCTTCCATACATGAGGCCACTCTCTTGGTTGGAGCTCCTGACTTGGGGGATTTTATTCCTTTTATCTCAATGCTTGATCTCCAAGGGCTAGGACGTCGTGCAAAAGGTGTTACTAAAGTCTTTGATGGATTCCTTGACATGATTATTAAACAACATCTCGAGTCGAATGATGAAAATCGAACAAAGGGTTGTTTTCTCGATGTTTTGCTGGATTCCAAGATTGATCACTCGAGTATCAAAGGCGTAATTATGGTCAGTAAACTTCATTTGTTCATGATTTTGTAAGGGATATTTAGTTTTGAATTATGGTTTACTGTATGCATCGTATTTGACTCTTTATTACAGACAAATATTTTGTATGATTGAGGATATTTAGCCTTCTATTACGTTTAACATAGGTACCATATTTGACCATTCAAATATCTAGATATTTGCTTTCCGGCTTACCATAATTATCATATTTACCCTTTTATTATAGTCAAATATCTTGTGTAATTGAGGAGATTTAGCCTTTACATTACCGTTTGACATAGGTACTATATTTGACCATTTATTATAAGCAAATATCTAGATATTTGCTTTCAATTACTACTTACATCATTATTATATTTGTCCTTTTATTATAGTCAAATATCTGGTATAATTGAGGATATTTAGTCTTCCATTATGATTTATCATAATTACCATATTTAACTCTTTATTATAGTCAAATATCTCCGGCTTACCATAGTTATCATAAGTGTCCTTGCACTACCTTCAAATAGTTATCATATTTAGCCTTCCATTAACATTTAATATAGGTACTACTTTTTATTATAGACAAATATTTGGATATTCGTAATCTATTACAGGTTATCATGACGATGATAAGACAAATATCTTGTATCCATTTATTACTATTTTCACACCCTTTATAATTTATTTGATTCGGTAAATGATTATAAACAAAGAACGTTTACTATCATTTAGTCATCTTAGCAAATTCTTTTTTTTTTTTTTTTTTTTTGGGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTGTAGGACTTGATTATTGGAGGAGTGGATAGTTCAAGAACTACAATCGATTGGGCATTATCCGAGCTAATCAAGCATCCCCATACAATGAAGAAAGTACAGAAGGAATTAGAAAACGTGGTTGGTTTAGGCAAAATGGTGGAAGAATCAGATTTGAGAGAGCTGAAATACTTAGAGATGGTCATAAAGGAGACCTTTAGAATGCATCCTCCCATACCATTAATTCCACGCAAGTGTATTGAAGATTGTATTGTCAATGGGTATCATATTCCTAAGAATTCCGAGGTATTAATAAACGAATGGGCAATTGGACGAGATCCATGTGTTTGGTTTGATCCTCTAACTTTTTTCCCGGAAAGATTTGCGAATAGAAAAATGGATACGAGAGGGAAAGATTTTCAATTGCATCCATTTGGGTATGGGTGCAGAAATTGTCCTGGAATGCAATTAGGATTGGTTTTGGTTCAATTGGTTGTTGCTCAATTGGTCCATTGGTTTGATTTGGAGCTTCCTAATGGTATATCTCCGAGTGAATTGGATATGACAGAACAATTTGGTTTAACTTGTACCAGAGCTCATAATCTTAGAGTCATTCTCGTCCGTCGTGCTCCTAATTGA

mRNA sequence

ATGAATATCAAAAAGTTGCCTCCTGGTCCAAAAGGATTCCCCATTATTGGTACCATCCATCTATTGAAAAAGCTTGTTCATAGAGATCTTCATCACTTATCTCAACTCTACGGACCCATAATGCACATGAAATTGGGACTCAAATCCACTATTATTGTCTCTTCCTCTCATGCTGCTGAGCTCTTCCTCAAAGCCCATGACCCAGTTTTTGCAGGCCGCCCAGTTCGTTTAACCTTCAACCAACTGAGTTACCACCAAAAAAACATAGCCTTTGCTCAATATGGCCCTTATTGGCGCAATATGCGTAAAATATGCATACTTCAATTGTTTACAACTCTCAAAATGAACTCCTTTACGTCAATGAGAAGAGAAGAGGTTGGTTTGTTGATTGATCATCTTCAAGAGGCTGCAAAAATGCATGGTGTTGTGGATCTCAGCTCTCAAATCTCGTCTTTTATTGCTGATGTGATTTGTAGAATGGTGTTTGGGAGAAAATTTGTAGAGGAAGAATTTAAGGCTTCCATACATGAGGCCACTCTCTTGGTTGGAGCTCCTGACTTGGGGGATTTTATTCCTTTTATCTCAATGCTTGATCTCCAAGGGCTAGGACGTCGTGCAAAAGGTGTTACTAAAGTCTTTGATGGATTCCTTGACATGATTATTAAACAACATCTCGAGTCGAATGATGAAAATCGAACAAAGGGTTGTTTTCTCGATGTTTTGCTGGATTCCAAGATTGATCACTCGAGTATCAAAGGCGTAATTATGGACTTGATTATTGGAGGAGTGGATAGTTCAAGAACTACAATCGATTGGGCATTATCCGAGCTAATCAAGCATCCCCATACAATGAAGAAAGTACAGAAGGAATTAGAAAACGTGGTTGGTTTAGGCAAAATGGTGGAAGAATCAGATTTGAGAGAGCTGAAATACTTAGAGATGGTCATAAAGGAGACCTTTAGAATGCATCCTCCCATACCATTAATTCCACGCAAGTGTATTGAAGATTGTATTGTCAATGGGTATCATATTCCTAAGAATTCCGAGGTATTAATAAACGAATGGGCAATTGGACGAGATCCATGTGTTTGGTTTGATCCTCTAACTTTTTTCCCGGAAAGATTTGCGAATAGAAAAATGGATACGAGAGGGAAAGATTTTCAATTGCATCCATTTGGGTATGGGTGCAGAAATTGTCCTGGAATGCAATTAGGATTGGTTTTGGTTCAATTGGTTGTTGCTCAATTGGTCCATTGGTTTGATTTGGAGCTTCCTAATGGTATATCTCCGAGTGAATTGGATATGACAGAACAATTTGGTTTAACTTGTACCAGAGCTCATAATCTTAGAGTCATTCTCGTCCGTCGTGCTCCTAATTGA

Coding sequence (CDS)

ATGAATATCAAAAAGTTGCCTCCTGGTCCAAAAGGATTCCCCATTATTGGTACCATCCATCTATTGAAAAAGCTTGTTCATAGAGATCTTCATCACTTATCTCAACTCTACGGACCCATAATGCACATGAAATTGGGACTCAAATCCACTATTATTGTCTCTTCCTCTCATGCTGCTGAGCTCTTCCTCAAAGCCCATGACCCAGTTTTTGCAGGCCGCCCAGTTCGTTTAACCTTCAACCAACTGAGTTACCACCAAAAAAACATAGCCTTTGCTCAATATGGCCCTTATTGGCGCAATATGCGTAAAATATGCATACTTCAATTGTTTACAACTCTCAAAATGAACTCCTTTACGTCAATGAGAAGAGAAGAGGTTGGTTTGTTGATTGATCATCTTCAAGAGGCTGCAAAAATGCATGGTGTTGTGGATCTCAGCTCTCAAATCTCGTCTTTTATTGCTGATGTGATTTGTAGAATGGTGTTTGGGAGAAAATTTGTAGAGGAAGAATTTAAGGCTTCCATACATGAGGCCACTCTCTTGGTTGGAGCTCCTGACTTGGGGGATTTTATTCCTTTTATCTCAATGCTTGATCTCCAAGGGCTAGGACGTCGTGCAAAAGGTGTTACTAAAGTCTTTGATGGATTCCTTGACATGATTATTAAACAACATCTCGAGTCGAATGATGAAAATCGAACAAAGGGTTGTTTTCTCGATGTTTTGCTGGATTCCAAGATTGATCACTCGAGTATCAAAGGCGTAATTATGGACTTGATTATTGGAGGAGTGGATAGTTCAAGAACTACAATCGATTGGGCATTATCCGAGCTAATCAAGCATCCCCATACAATGAAGAAAGTACAGAAGGAATTAGAAAACGTGGTTGGTTTAGGCAAAATGGTGGAAGAATCAGATTTGAGAGAGCTGAAATACTTAGAGATGGTCATAAAGGAGACCTTTAGAATGCATCCTCCCATACCATTAATTCCACGCAAGTGTATTGAAGATTGTATTGTCAATGGGTATCATATTCCTAAGAATTCCGAGGTATTAATAAACGAATGGGCAATTGGACGAGATCCATGTGTTTGGTTTGATCCTCTAACTTTTTTCCCGGAAAGATTTGCGAATAGAAAAATGGATACGAGAGGGAAAGATTTTCAATTGCATCCATTTGGGTATGGGTGCAGAAATTGTCCTGGAATGCAATTAGGATTGGTTTTGGTTCAATTGGTTGTTGCTCAATTGGTCCATTGGTTTGATTTGGAGCTTCCTAATGGTATATCTCCGAGTGAATTGGATATGACAGAACAATTTGGTTTAACTTGTACCAGAGCTCATAATCTTAGAGTCATTCTCGTCCGTCGTGCTCCTAATTGA

Protein sequence

MNIKKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLKAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRREEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEEFKASIHEATLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFLDVLLDSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPLIPRKCIEDCIVNGYHIPKNSEVLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLVLVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVRRAPN
BLAST of CmaCh16G009180 vs. Swiss-Prot
Match: C7A12_PANGI (Cytochrome P450 CYP736A12 OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 9.7e-125
Identity = 223/464 (48.06%), Postives = 306/464 (65.95%), Query Frame = 1

Query: 5   KLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLK 64
           KLPPGP+G PIIG++H L  L HR L  L++ YGPIM M+LG   TI+VSS  AAELFLK
Sbjct: 32  KLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPIMSMRLGSVPTIVVSSPQAAELFLK 91

Query: 65  AHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRRE 124
            HD +FA RP       +SY    ++F  YGP+WRN+RK  +L+L T  K+NSF  MRRE
Sbjct: 92  THDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRNIRKFVVLELLTPAKINSFVGMRRE 151

Query: 125 EVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGR-KFVEEEFKASIHEATLLVG 184
           E+G ++  ++EA+  + VVDLS+++++ I ++  R++ GR K    + K  ++EA  L G
Sbjct: 152 ELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRLLLGRTKDDRYDLKGIMNEALTLAG 211

Query: 185 APDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFLDVLLD 244
             ++ DF+PF+  LD+QGL R+ K   K  D  L+ II +H +++      G F+D +L 
Sbjct: 212 RFNIADFVPFLGPLDIQGLTRQFKDTGKRLDKILEFIIDEHEQNSSNGNASGDFIDDMLS 271

Query: 245 SK-------------IDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKE 304
            K             ID S IK +++D+I   +D+S T+I+W L+ELIKHP  MKK Q+E
Sbjct: 272 LKNKPSNTHDELSKVIDRSVIKAIMIDIISAAIDTSDTSIEWILTELIKHPRAMKKCQEE 331

Query: 305 LENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPLI-PRKCIEDCIVNGYHIPKNSE 364
           ++ VVG+ +MVEE+DL  L+Y+ MV+KE  R+HP  PL+ P + +ED  +NGY IPK S 
Sbjct: 332 IDAVVGVDRMVEETDLPNLEYVYMVVKEGLRLHPVAPLLGPHESMEDITINGYFIPKQSR 391

Query: 365 VLINEWAIGRDPCVWFDPL-TFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLVL 424
           V++N WA+GRDP VW +    F PERF    +D RG+DFQL PFG G R CPGMQLGL+ 
Sbjct: 392 VIVNSWALGRDPNVWSENAEEFLPERFEGSNVDVRGRDFQLLPFGSGRRGCPGMQLGLIT 451

Query: 425 VQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVI 453
           VQLVVA+LVH FD  LPNG +P  LDMTE+FGLT  R  +L  +
Sbjct: 452 VQLVVARLVHCFDWNLPNGTTPDNLDMTEKFGLTTPRVKHLLAV 495

BLAST of CmaCh16G009180 vs. Swiss-Prot
Match: C71A1_PERAE (Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 371.3 bits (952), Expect = 1.5e-101
Identity = 191/469 (40.72%), Postives = 279/469 (59.49%), Query Frame = 1

Query: 6   LPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLKA 65
           LPP P   PIIG +H L  L HR L  L+   GP++ + LG   T+IVS++  AE  LK 
Sbjct: 31  LPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLILLHLGHIPTLIVSTAEIAEEILKT 90

Query: 66  HDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRREE 125
           HD +FA RP      ++ Y   ++AF+ YG YWR +RKIC+L+L +  ++NS+ S+R EE
Sbjct: 91  HDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNSYRSIREEE 150

Query: 126 VGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEE-----FKASIHEATL 185
           VGL+++ + ++      V+LS  +    +  I R+ FG+K+  EE     F     E T 
Sbjct: 151 VGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAFGKKYEGEEERKNKFADLATELTT 210

Query: 186 LVGAPDLGDFIPFISMLD-LQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGC--- 245
           L+GA  +GD+ P  + +D L G+  R K      D F+D +I  HL S   N + G    
Sbjct: 211 LMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDHVIDDHLLSRKANGSDGVEQK 270

Query: 246 -FLDVLLDSKIDHS--------SIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKV 305
             +DVLL  + D S        ++K VI+D+  GG D++  T++WA++ELIKHP  M+K 
Sbjct: 271 DLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTDTTAVTLEWAMAELIKHPDVMEKA 330

Query: 306 QKELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIP-LIPRKCIEDCIVNGYHIPK 365
           Q+E+  VVG    VEE DL +L YL+++IKET R+HP  P L+PR+   D ++ GYHIP 
Sbjct: 331 QQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLHPVAPLLVPRESTRDVVIRGYHIPA 390

Query: 366 NSEVLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGL 425
            + V IN WAIGRDP  W +   F PERF N  +D +G+DFQL PFG G R CPG+  G+
Sbjct: 391 KTRVFINAWAIGRDPKSWENAEEFLPERFVNNSVDFKGQDFQLIPFGAGRRGCPGIAFGI 450

Query: 426 VLVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVR 456
             V++ +A L++WF+ ELP  ++  +LDM+E  G+T      L+++  R
Sbjct: 451 SSVEISLANLLYWFNWELPGDLTKEDLDMSEAVGITVHMKFPLQLVAKR 499

BLAST of CmaCh16G009180 vs. Swiss-Prot
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.9e-96
Identity = 191/478 (39.96%), Postives = 273/478 (57.11%), Query Frame = 1

Query: 5   KLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLK 64
           +LPPGP  +PIIG +  +    HR L  +   YGPI+H++LG    ++ +S   AE FLK
Sbjct: 32  RLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPILHLRLGFVDVVVAASKSVAEQFLK 91

Query: 65  AHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRRE 124
            HD  FA RP       ++Y+ +++ FA YG  WR +RKI  + LF+   +  F  +R+E
Sbjct: 92  IHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLRKISSVHLFSAKALEDFKHVRQE 151

Query: 125 EVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFV-------EEEFKASIHE 184
           EVG L   L         V+L   ++  + + + R + GR+          +EF++ + E
Sbjct: 152 EVGTLTRELVRVGTK--PVNLGQLVNMCVVNALGREMIGRRLFGADADHKADEFRSMVTE 211

Query: 185 ATLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCF 244
              L G  ++GDF+P +  LDLQG+  + K + K FD FL  I+K+H E N +++     
Sbjct: 212 MMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFLSSILKEH-EMNGQDQKHTDM 271

Query: 245 LDVLLDSK----------IDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKV 304
           L  L+  K          +  + IK +++++   G D+S +T+DWA++ELI+HP  M K 
Sbjct: 272 LSTLISLKGTDLDGDGGSLTDTEIKALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKA 331

Query: 305 QKELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPL-IPRKCIEDCIVNGYHIPK 364
           Q+EL+ VVG  + V ESD+ +L YL+ VIKE FR+HPP PL +P    E C +NGYHIPK
Sbjct: 332 QEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPK 391

Query: 365 NSEVLINEWAIGRDPCVWFDPLTFFPERF----ANRKMDTRGKDFQLHPFGYGCRNCPGM 424
            S +L N WAI RDP  W DPL F PERF        +D +G DF+L PFG G R C G+
Sbjct: 392 GSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGL 451

Query: 425 QLGLVLVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRV-ILVRRAPN 460
            LGL  +Q + A LV  FD EL  G++P +L+M E +GLT  RA  L V    R APN
Sbjct: 452 SLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPN 506

BLAST of CmaCh16G009180 vs. Swiss-Prot
Match: C84A1_ARATH (Cytochrome P450 84A1 OS=Arabidopsis thaliana GN=CYP84A1 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.3e-96
Identity = 180/478 (37.66%), Postives = 282/478 (59.00%), Query Frame = 1

Query: 7   PPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLKAH 66
           PPGP+G+PIIG + ++ +L HR L +L++ YG + H+++G      VSS   A   L+  
Sbjct: 41  PPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQ 100

Query: 67  DPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRREEV 126
           D VF+ RP  +  + L+Y + ++AFA YGP+WR MRK+C++++F+  +  S+ S+R +EV
Sbjct: 101 DSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQMRKVCVMKVFSRKRAESWASVR-DEV 160

Query: 127 GLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFV--EEEFKASIHEATLLVGA 186
             ++  +  +  +   +++  QI +   ++  R  FG      ++EF   + E + L GA
Sbjct: 161 DKMVRSV--SCNVGKPINVGEQIFALTRNITYRAAFGSACEKGQDEFIRILQEFSKLFGA 220

Query: 187 PDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLES----------------- 246
            ++ DFIP+   +D QG+ +R        DGF+D II +H++                  
Sbjct: 221 FNVADFIPYFGWIDPQGINKRLVKARNDLDGFIDDIIDEHMKKKENQNAVDDGDVVDTDM 280

Query: 247 --------NDENRTKGCFLDVLLDSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIK 306
                   ++E +      D+    K+   +IK +IMD++ GG ++  + I+WAL+EL++
Sbjct: 281 VDDLLAFYSEEAKLVSETADLQNSIKLTRDNIKAIIMDVMFGGTETVASAIEWALTELLR 340

Query: 307 HPHTMKKVQKELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPLIPRKCIEDCIV 366
            P  +K+VQ+EL  VVGL + VEESD+ +L YL+  +KET RMHPPIPL+  +  ED  +
Sbjct: 341 SPEDLKRVQQELAEVVGLDRRVEESDIEKLTYLKCTLKETLRMHPPIPLLLHETAEDTSI 400

Query: 367 NGYHIPKNSEVLINEWAIGRDPCVWFDPLTFFPERFANRKM-DTRGKDFQLHPFGYGCRN 426
           +G+ IPK S V+IN +AIGRDP  W DP TF P RF    + D +G +F+  PFG G R+
Sbjct: 401 DGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLEPGVPDFKGSNFEFIPFGSGRRS 460

Query: 427 CPGMQLGLVLVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVRR 457
           CPGMQLGL  + L VA ++H F  +LP+G+ PSELDM + FGLT  +A  L  +   R
Sbjct: 461 CPGMQLGLYALDLAVAHILHCFTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVPTTR 515

BLAST of CmaCh16G009180 vs. Swiss-Prot
Match: C75A1_PINTA (Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 6.8e-94
Identity = 182/464 (39.22%), Postives = 285/464 (61.42%), Query Frame = 1

Query: 4   KKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFL 63
           ++LPPGP  +PIIG  H ++  +HR L +L++ YGPI+ ++ G   T++VSSS  A+ FL
Sbjct: 40  ERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILFLRFGSVPTVVVSSSEKAKHFL 99

Query: 64  KAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRR 123
           K HD +FA RP         Y+ K+IAF+ YG +WR MRKIC+L+L T+ ++ SF  +R+
Sbjct: 100 KTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMRKICVLELLTSKRIESFKHVRQ 159

Query: 124 EEVGLLIDHLQEAAKMHGV-VDLSSQISSFIADVICRMVFGRKFVEEEFKAS-------I 183
           EE+  +I  + E ++   + V++S  IS+ +A+++ R++  +KF + +  A        +
Sbjct: 160 EELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRILARKKFSDNDLGADGKGFADLV 219

Query: 184 HEATLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESN---DENR 243
            E ++ VG+ ++GDFIP++  LDLQG+ R  K     FD F + +I +H+ ++   +   
Sbjct: 220 VEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANARFDAFAEKMIDEHINASTIRNGEA 279

Query: 244 TKGC----FLDVLLD--------SKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKH 303
             GC     +DVLL+        +K+    IK +  +L   G+++S   ++WA+SEL++H
Sbjct: 280 DAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITYELFSAGMETSANVLEWAMSELLRH 339

Query: 304 PHTMKKVQKELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPL-IPRKCIEDCIV 363
           PH MKK+Q+E+E+VVG    V+ESDL  + YL  V+KET R++P +PL +P + +E   V
Sbjct: 340 PHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVKETLRLYPSLPLALPHESLEAVTV 399

Query: 364 NGYHIPKNSEVLINEWAIGRDPCVW-FDPLTFFPERFANRK---MDTRG--KDFQLHPFG 423
            GY+IPK + V++N WAIGRDP VW  D   F PERF   +   +D  G   DF++ PFG
Sbjct: 400 GGYYIPKKTMVIMNLWAIGRDPSVWGADASEFKPERFMQMEENGIDLSGGQSDFRMLPFG 459

Query: 424 YGCRNCPGMQLGLVLVQLVVAQLVHWFDLELPNGISPSELDMTE 438
            G R CPG  + ++ V+  +AQL+H FD  +     PSELDM E
Sbjct: 460 AGRRTCPGSAMAILTVEFTLAQLLHTFDWRVEG--DPSELDMKE 501

BLAST of CmaCh16G009180 vs. TrEMBL
Match: A0A0A0KGD3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501350 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 7.4e-148
Identity = 265/472 (56.14%), Postives = 340/472 (72.03%), Query Frame = 1

Query: 4   KKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFL 63
           K LPPGPKGFPI G++HL+ KL HRDLH LSQ YGPIMHMKLGL  TIIVSS HAA+LFL
Sbjct: 28  KNLPPGPKGFPIFGSLHLIGKLPHRDLHRLSQKYGPIMHMKLGLVHTIIVSSPHAAKLFL 87

Query: 64  KAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRR 123
           K HD VFA RP+  T + ++Y +K++ FA YG YWRN+RK+C L+LF++LK+NSF SMR+
Sbjct: 88  KTHDHVFASRPLIHTSSIMTYGKKDLVFAPYGSYWRNIRKMCTLELFSSLKINSFKSMRK 147

Query: 124 EEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEE-----FKASIHEA 183
           +EV  LI++L+ A+    VV LSS+++S IAD+ C M FG+K+ +EE     FKA I E 
Sbjct: 148 KEVRELIEYLKTASTDRMVVRLSSKVTSLIADMTCLMAFGKKYRDEEFGERGFKAVIQEG 207

Query: 184 TLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFL 243
             LV AP+L D+IPF++  D+QGL RRA  V K FDGF + II++H+ES D NR K  F+
Sbjct: 208 MQLVIAPNLADYIPFVAPFDIQGLNRRATFVLKEFDGFFERIIEEHIESKDGNRNKD-FM 267

Query: 244 DVLLD----------SKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQ 303
           D LLD           +ID S+IK + +D++   +D+S T IDWAL+ELIKHP  MK++Q
Sbjct: 268 DHLLDIMMSSQDPDGYQIDRSNIKAIALDVLSAAMDTSATAIDWALAELIKHPQAMKELQ 327

Query: 304 KELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIP-LIPRKCIEDCIVNGYHIPKN 363
            ELE VVGL +MVEES L  L+YL MVIKE  R+HPP P L+P + +EDC V+G+HIPK 
Sbjct: 328 VELEKVVGLNRMVEESHLEHLQYLGMVIKEVLRLHPPAPLLVPHESLEDCTVDGFHIPKK 387

Query: 364 SEVLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLV 423
           S + +N W+IG+DP VW DP  FFPERF +   D +G+DF L PFG G R+CPG+ LGL+
Sbjct: 388 SRIFVNAWSIGQDPNVWIDPQKFFPERFIHSLADVKGRDFHLIPFGSGRRSCPGIHLGLL 447

Query: 424 LVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVRRAPN 460
           +V LVVA LVH FD ELPN +  ++LDM E+FGLTC RA  L +I   R  N
Sbjct: 448 IVSLVVANLVHCFDWELPNCMLATDLDMEEEFGLTCPRAQELMLIPTYRLRN 498

BLAST of CmaCh16G009180 vs. TrEMBL
Match: B9HFV3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s06240g PE=3 SV=2)

HSP 1 Score: 531.6 bits (1368), Expect = 9.7e-148
Identity = 260/464 (56.03%), Postives = 338/464 (72.84%), Query Frame = 1

Query: 4   KKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFL 63
           K+LPPGP+GFPI G++HLL K  HR LH L+Q YGPIMH++LGL  TI+VSS  AAELFL
Sbjct: 25  KRLPPGPRGFPIFGSLHLLGKFPHRALHQLAQKYGPIMHLRLGLVPTIVVSSPEAAELFL 84

Query: 64  KAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRR 123
           K HD VFAGRP       +SY QK +AFAQYG YWRNMRK+C ++L ++LK+ SF  MR 
Sbjct: 85  KTHDLVFAGRPPHEAARYISYGQKGMAFAQYGSYWRNMRKMCTVELLSSLKITSFKPMRM 144

Query: 124 EEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEE-----FKASIHEA 183
           EE+ LLI ++QEAA+    VD+S+++SS  AD+ CRMVFG+K+V+E+     FK+ + E 
Sbjct: 145 EELDLLIKYIQEAAQERVAVDMSAKVSSLSADMSCRMVFGKKYVDEDLDERGFKSVMQEV 204

Query: 184 TLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFL 243
             L  AP LGD+IP I+ LDLQGL +R   ++KVFD FLD II +H++  ++ + K  F+
Sbjct: 205 MHLTAAPHLGDYIPQIAALDLQGLTKRMNAISKVFDVFLDKIIDEHVQYQEKGKNKD-FV 264

Query: 244 DVLL--------DSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKE 303
           DV+L        +  +D   +K  ++D+++G +D+S T IDWA SELIK+P  MKK+QKE
Sbjct: 265 DVMLSLMKSEENEYLVDQGCMKATMLDMLVGSMDTSATVIDWAFSELIKNPRVMKKLQKE 324

Query: 304 LENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPL-IPRKCIEDCIVNGYHIPKNSE 363
           +E VVG  +MVEESDL  L+YL+MV+KET R+HP  PL IP +  EDC+VNG+HIPK S 
Sbjct: 325 IEEVVGKQRMVEESDLERLEYLDMVVKETLRLHPAGPLMIPHEATEDCVVNGFHIPKKSH 384

Query: 364 VLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLVLV 423
           V+IN WAIGRDP  W D   F+PERF    +D RG+DFQL PFG G R+CPGMQLGL +V
Sbjct: 385 VIINVWAIGRDPKAWTDAEKFYPERFVGSDIDVRGRDFQLIPFGTGRRSCPGMQLGLTVV 444

Query: 424 QLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVIL 454
           +LV+AQLVH FD ELPNGI PSE+DMTE+FGL   R+ +L  I+
Sbjct: 445 RLVLAQLVHCFDWELPNGILPSEVDMTEEFGLVLCRSKHLIKIM 487

BLAST of CmaCh16G009180 vs. TrEMBL
Match: V4SVX2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027549mg PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.8e-146
Identity = 260/463 (56.16%), Postives = 332/463 (71.71%), Query Frame = 1

Query: 4   KKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFL 63
           KKLPPGPKGFPI G +HLL +  HR LH L++ YGPIMH++LGL  TI+VSS  AAELFL
Sbjct: 30  KKLPPGPKGFPIFGCLHLLGQFPHRALHKLAERYGPIMHLRLGLTPTIVVSSPQAAELFL 89

Query: 64  KAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRR 123
           K HD +FA RP       +SY QK +A + YG YWR +RKIC L+L ++ K+NSF +MR+
Sbjct: 90  KTHDLIFASRPPLKAAKHISYEQKGLALSPYGSYWRTIRKICTLELLSSFKINSFQAMRK 149

Query: 124 EEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEE-----FKASIHEA 183
           EE+ LLI  ++EAA    VVDLS+++S+  AD+ CRMVFG+K+ ++E     FK  I E 
Sbjct: 150 EELDLLIASIKEAACARCVVDLSTKVSAMSADMTCRMVFGKKYSDDEFDERGFKTVIQEG 209

Query: 184 TLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFL 243
             L   P+LGD+IP I+ LDLQGL +R K V KVFDGFL+ II +H++S DENRTK  F+
Sbjct: 210 MELAAKPNLGDYIPQIAGLDLQGLTKRMKSVAKVFDGFLEKIIDEHVQSKDENRTKD-FV 269

Query: 244 DVLL--------DSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKE 303
           DV+L        + KI+   IK +I+D++   +D+S T ++WALSELIKHP  MKK+QKE
Sbjct: 270 DVMLSFEGSEETEHKIEREHIKAIILDMLSAAMDTSATAVEWALSELIKHPKVMKKLQKE 329

Query: 304 LENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIP-LIPRKCIEDCIVNGYHIPKNSE 363
           LE +VGL +MVEESDL  L+YL+MV+KET R+HP  P LIP +  EDC VNG++IPKNS 
Sbjct: 330 LEKIVGLCRMVEESDLENLEYLDMVVKETLRLHPVAPLLIPHESTEDCTVNGFYIPKNSR 389

Query: 364 VLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLVLV 423
           V++N WAIGRD   W DP  FFPERF    +D RG DFQL PFG G R CPGMQLGL +V
Sbjct: 390 VIVNVWAIGRDTESWSDPEIFFPERFVGSSIDLRGHDFQLLPFGSGRRGCPGMQLGLTIV 449

Query: 424 QLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVI 453
           + V+AQLVH FD ELP G+ P+ELD+TE+F L   +A +L  I
Sbjct: 450 KQVIAQLVHCFDWELPGGMLPTELDLTEEFSLVTPKAKHLLAI 491

BLAST of CmaCh16G009180 vs. TrEMBL
Match: B9HFW2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s06310g PE=3 SV=2)

HSP 1 Score: 525.8 bits (1353), Expect = 5.3e-146
Identity = 258/463 (55.72%), Postives = 337/463 (72.79%), Query Frame = 1

Query: 4   KKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFL 63
           K+LPPGP+GFPI G++HLL K  HR  H L+Q YGPIMH++LGL  TI+VSS  AAELFL
Sbjct: 25  KRLPPGPRGFPIFGSLHLLGKFPHRAFHQLAQKYGPIMHLRLGLVPTIVVSSPEAAELFL 84

Query: 64  KAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRR 123
           K HD VFAGRP   +   +SY QK++ F+QYG YWRNMRK+C ++L ++LK+ SF S R 
Sbjct: 85  KTHDLVFAGRPPHESARYISYGQKSVTFSQYGSYWRNMRKMCTVELLSSLKITSFKSTRM 144

Query: 124 EEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEE-----FKASIHEA 183
           EE+ LLI ++QEAA+    VDLS+++SS  AD+ CRMVFG+K+++E+     FK+ + E 
Sbjct: 145 EELDLLIKYIQEAAQERVAVDLSAKVSSLSADMSCRMVFGKKYLDEDLDERGFKSVMQEV 204

Query: 184 TLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFL 243
             L  AP+LGD+IP I  LDLQGL +R K ++KV D F+  II +H +  ++ + K  F+
Sbjct: 205 MHLSAAPNLGDYIPQIGALDLQGLTKRMKAISKVLDLFVSKIIDEHAQYQEKGKNKD-FV 264

Query: 244 DVLL--------DSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKE 303
           DV+L        +  +D   +K +++D++ G +D+S T IDWA SELIK+P  MKKVQKE
Sbjct: 265 DVMLSCMKSEENEYLVDQGCMKAIMLDMLAGSMDTSSTVIDWAFSELIKNPRVMKKVQKE 324

Query: 304 LENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIP-LIPRKCIEDCIVNGYHIPKNSE 363
           LE VVG  +MVEESDL  L+YL+MV+KETFR+HP  P LIP + +EDCIVNG+HIPK S 
Sbjct: 325 LEEVVGKQRMVEESDLESLEYLDMVVKETFRLHPVGPLLIPHEAMEDCIVNGFHIPKKSH 384

Query: 364 VLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLVLV 423
           V+IN WAIGRDP  W D   F+PERF    +D RG++FQL PFG G R+CPGMQLGL +V
Sbjct: 385 VIINVWAIGRDPKAWTDAENFYPERFVGSDIDVRGRNFQLIPFGAGRRSCPGMQLGLTVV 444

Query: 424 QLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVI 453
           +LV+AQLVH FD ELPNGI PSE+DMTE+FGL   R+ +L  I
Sbjct: 445 RLVLAQLVHCFDWELPNGILPSEVDMTEEFGLVICRSKHLVAI 486

BLAST of CmaCh16G009180 vs. TrEMBL
Match: M5WID8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004664mg PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.2e-145
Identity = 259/464 (55.82%), Postives = 332/464 (71.55%), Query Frame = 1

Query: 4   KKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFL 63
           K+LPPGP+GFPI G++HLL +  ++DLH L++ YG IM+M+LGL STI++SS  AAELFL
Sbjct: 26  KRLPPGPRGFPIFGSLHLLGEFPNKDLHRLARKYGDIMYMRLGLMSTIVISSPEAAELFL 85

Query: 64  KAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRR 123
           K HD VFA RP       +S+ QKN++F++YG YWR+ RK+C ++L +  K+NSF SMRR
Sbjct: 86  KTHDLVFASRPPHEGSKHISFGQKNLSFSEYGVYWRDTRKMCTIELLSNHKINSFKSMRR 145

Query: 124 EEVGLLIDHLQEAAKMHGV-VDLSSQISSFIADVICRMVFGRKFVEEEF-----KASIHE 183
           EEV L ++ ++ AA   GV VDLS ++SS   D+ CRMV G+K+ +EEF     K+ + E
Sbjct: 146 EEVSLCVESIRAAANNIGVAVDLSDKVSSLSVDMSCRMVLGKKYRDEEFDERGFKSVVRE 205

Query: 184 ATLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCF 243
              L  AP+LGD+IPFI+ LDLQG  +R K V K FD   +  I++HL+ ND  RTK  F
Sbjct: 206 GIQLASAPNLGDYIPFIAPLDLQGFTKRMKSVNKAFDNLFEKSIEEHLQPNDGERTKD-F 265

Query: 244 LDVLL--------DSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQK 303
           +DV++        + +I+   IK +++D+++  +D+S TTI+WALSEL++HP  MKKVQK
Sbjct: 266 VDVMVGFMGSEESEYRIERPHIKAIMLDMLVASMDTSSTTIEWALSELMRHPKAMKKVQK 325

Query: 304 ELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPL-IPRKCIEDCIVNGYHIPKNS 363
           ELENVVGL KMVEESDL +L YL MV+KETFR+HP  PL +P   IEDC VNGYHIPKNS
Sbjct: 326 ELENVVGLDKMVEESDLEKLDYLNMVVKETFRLHPVAPLLVPHASIEDCTVNGYHIPKNS 385

Query: 364 EVLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLVL 423
            VLIN WAIGRDP  W DP  F PERF    +D RG  FQL PFG G R CPG+QLGL +
Sbjct: 386 RVLINVWAIGRDPNAWTDPEKFIPERFEGSSVDVRGNHFQLIPFGSGRRRCPGIQLGLTV 445

Query: 424 VQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVI 453
           VQLV+AQLVH FD ELPN + P ELDMTE+FG T  RA +L  I
Sbjct: 446 VQLVLAQLVHCFDWELPNNMLPDELDMTEEFGFTVPRAKHLLAI 488

BLAST of CmaCh16G009180 vs. TAIR10
Match: AT5G07990.1 (AT5G07990.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 354.4 bits (908), Expect = 1.1e-97
Identity = 191/478 (39.96%), Postives = 273/478 (57.11%), Query Frame = 1

Query: 5   KLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLK 64
           +LPPGP  +PIIG +  +    HR L  +   YGPI+H++LG    ++ +S   AE FLK
Sbjct: 32  RLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPILHLRLGFVDVVVAASKSVAEQFLK 91

Query: 65  AHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRRE 124
            HD  FA RP       ++Y+ +++ FA YG  WR +RKI  + LF+   +  F  +R+E
Sbjct: 92  IHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLRKISSVHLFSAKALEDFKHVRQE 151

Query: 125 EVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFV-------EEEFKASIHE 184
           EVG L   L         V+L   ++  + + + R + GR+          +EF++ + E
Sbjct: 152 EVGTLTRELVRVGTK--PVNLGQLVNMCVVNALGREMIGRRLFGADADHKADEFRSMVTE 211

Query: 185 ATLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCF 244
              L G  ++GDF+P +  LDLQG+  + K + K FD FL  I+K+H E N +++     
Sbjct: 212 MMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFLSSILKEH-EMNGQDQKHTDM 271

Query: 245 LDVLLDSK----------IDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKV 304
           L  L+  K          +  + IK +++++   G D+S +T+DWA++ELI+HP  M K 
Sbjct: 272 LSTLISLKGTDLDGDGGSLTDTEIKALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKA 331

Query: 305 QKELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPL-IPRKCIEDCIVNGYHIPK 364
           Q+EL+ VVG  + V ESD+ +L YL+ VIKE FR+HPP PL +P    E C +NGYHIPK
Sbjct: 332 QEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPK 391

Query: 365 NSEVLINEWAIGRDPCVWFDPLTFFPERF----ANRKMDTRGKDFQLHPFGYGCRNCPGM 424
            S +L N WAI RDP  W DPL F PERF        +D +G DF+L PFG G R C G+
Sbjct: 392 GSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGL 451

Query: 425 QLGLVLVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRV-ILVRRAPN 460
            LGL  +Q + A LV  FD EL  G++P +L+M E +GLT  RA  L V    R APN
Sbjct: 452 SLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPN 506

BLAST of CmaCh16G009180 vs. TAIR10
Match: AT4G36220.1 (AT4G36220.1 ferulic acid 5-hydroxylase 1)

HSP 1 Score: 353.6 bits (906), Expect = 1.8e-97
Identity = 180/478 (37.66%), Postives = 282/478 (59.00%), Query Frame = 1

Query: 7   PPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLKAH 66
           PPGP+G+PIIG + ++ +L HR L +L++ YG + H+++G      VSS   A   L+  
Sbjct: 41  PPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQ 100

Query: 67  DPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRREEV 126
           D VF+ RP  +  + L+Y + ++AFA YGP+WR MRK+C++++F+  +  S+ S+R +EV
Sbjct: 101 DSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQMRKVCVMKVFSRKRAESWASVR-DEV 160

Query: 127 GLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFV--EEEFKASIHEATLLVGA 186
             ++  +  +  +   +++  QI +   ++  R  FG      ++EF   + E + L GA
Sbjct: 161 DKMVRSV--SCNVGKPINVGEQIFALTRNITYRAAFGSACEKGQDEFIRILQEFSKLFGA 220

Query: 187 PDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLES----------------- 246
            ++ DFIP+   +D QG+ +R        DGF+D II +H++                  
Sbjct: 221 FNVADFIPYFGWIDPQGINKRLVKARNDLDGFIDDIIDEHMKKKENQNAVDDGDVVDTDM 280

Query: 247 --------NDENRTKGCFLDVLLDSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIK 306
                   ++E +      D+    K+   +IK +IMD++ GG ++  + I+WAL+EL++
Sbjct: 281 VDDLLAFYSEEAKLVSETADLQNSIKLTRDNIKAIIMDVMFGGTETVASAIEWALTELLR 340

Query: 307 HPHTMKKVQKELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPLIPRKCIEDCIV 366
            P  +K+VQ+EL  VVGL + VEESD+ +L YL+  +KET RMHPPIPL+  +  ED  +
Sbjct: 341 SPEDLKRVQQELAEVVGLDRRVEESDIEKLTYLKCTLKETLRMHPPIPLLLHETAEDTSI 400

Query: 367 NGYHIPKNSEVLINEWAIGRDPCVWFDPLTFFPERFANRKM-DTRGKDFQLHPFGYGCRN 426
           +G+ IPK S V+IN +AIGRDP  W DP TF P RF    + D +G +F+  PFG G R+
Sbjct: 401 DGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLEPGVPDFKGSNFEFIPFGSGRRS 460

Query: 427 CPGMQLGLVLVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVRR 457
           CPGMQLGL  + L VA ++H F  +LP+G+ PSELDM + FGLT  +A  L  +   R
Sbjct: 461 CPGMQLGLYALDLAVAHILHCFTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVPTTR 515

BLAST of CmaCh16G009180 vs. TAIR10
Match: AT2G45560.1 (AT2G45560.1 cytochrome P450, family 76, subfamily C, polypeptide 1)

HSP 1 Score: 335.9 bits (860), Expect = 4.0e-92
Identity = 177/473 (37.42%), Postives = 281/473 (59.41%), Query Frame = 1

Query: 6   LPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLKA 65
           LPPGP   PIIG IHL+ K  HR    LS+ YGP+M +KLG  +T++++S  AA   L+ 
Sbjct: 38  LPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRT 97

Query: 66  HDPVFAGRPVRLTFNQLSYHQKNIAFA-QYGPYWRNMRKICILQLFTTLKMNSFTSMRRE 125
           HD + + R        +++   ++ +       WR +R++ + QL +  ++ +  ++R  
Sbjct: 98  HDQILSARSPTNAVRSINHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMN 157

Query: 126 EVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEEFKASIH--EATLL- 185
           +V  L+  + E++     VD+S        ++I  ++F         KASI+  + T++ 
Sbjct: 158 KVKELVSFISESSDREESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVIS 217

Query: 186 ----VGAPDLGDFIPFISMLDLQGLGRRAKGVT----KVFDGFLDM-IIKQHLESNDENR 245
                G PD  ++ PF+  LDLQG  +  K  T    +VF GF+D  I ++  ++N ++ 
Sbjct: 218 VMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDV 277

Query: 246 TKGCFLDVLLDSKIDHSS-----IKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKV 305
           +K  F+D LLD K D S      I+ +++D+   G D+S +T++WA++EL+K+P TM K 
Sbjct: 278 SKNDFVDNLLDYKGDESELSISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKA 337

Query: 306 QKELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPL-IPRKCIEDCIVNGYHIPK 365
           Q E++ V+G   +VEESD+ +L YL+ V+KETFR+H P+PL IPRK   D  + G+ + K
Sbjct: 338 QAEIDCVIGQNGIVEESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLK 397

Query: 366 NSEVLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGL 425
           +++VL+N WAIGRDP VW +P  F PERF  + MD RG+D++L PFG G R CPGM L +
Sbjct: 398 DTQVLVNVWAIGRDPSVWDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAM 457

Query: 426 VLVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVRRAPN 460
             V L++A L++ FD +LP G+   +LDM E FGLT  + + L  + V++  N
Sbjct: 458 KTVSLMLASLLYSFDWKLPKGVLSEDLDMDETFGLTLHKTNPLHAVPVKKRAN 510

BLAST of CmaCh16G009180 vs. TAIR10
Match: AT3G26310.1 (AT3G26310.1 cytochrome P450, family 71, subfamily B, polypeptide 35)

HSP 1 Score: 334.7 bits (857), Expect = 8.8e-92
Identity = 172/474 (36.29%), Postives = 274/474 (57.81%), Query Frame = 1

Query: 2   NIKKLP-----PGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSS 61
           N KK P     P P GFPIIG +H + +L H+ L  LS+ YGP+MH+ LG   T++VSSS
Sbjct: 21  NHKKHPKYRQFPCPPGFPIIGNLHQIGELPHQTLWKLSKKYGPVMHLMLGRVPTVVVSSS 80

Query: 62  HAAELFLKAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMN 121
             A   L+ HD     RP      +LSY+  +IAF+ Y  YW+ +RK+C+ +LF+T +++
Sbjct: 81  DTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWKEVRKLCVQELFSTKQVH 140

Query: 122 SFTSMRREEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFV-----EEEF 181
           S   ++ EEV  +ID + E+A     V+L+++       V+CR  FG  F       + F
Sbjct: 141 SIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRTAFGVSFEGTVLNSDRF 200

Query: 182 KASIHEATLLVGAPDLGDFIPFISML--DLQGLGRRAKGVTKVFDGFLDMIIKQHLESND 241
              + EA  ++G+    DFIP++  +   L GL  R +   +  + F + +   H E   
Sbjct: 201 NKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKRDLNAFFEQMFDLHKEGKK 260

Query: 242 ENRTKGCFLDVLL----------DSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIK 301
           E      F+D+LL          + K+  + IK +++D+++ G+D+S  T+ WA++EL +
Sbjct: 261 EGNED--FVDLLLRLEKEEAVLGNDKLTRNHIKAILLDVLLAGIDTSAITMTWAMTELAR 320

Query: 302 HPHTMKKVQKELENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIP-LIPRKCIEDCI 361
           +P  MKKVQ E+   +G   M+   D+ +L+YL+MVIKET+R+HP  P L+PR+ + +  
Sbjct: 321 NPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVIKETWRLHPTTPLLLPREAMSEFD 380

Query: 362 VNGYHIPKNSEVLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRN 421
           +NGY IP  + + +N WAIGRDP  W DP  F PERF +  +D +G+ F+L PFG G R 
Sbjct: 381 INGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMDNNIDAKGQHFELLPFGGGRRI 440

Query: 422 CPGMQLGLVLVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVI 453
           CP + +G  +V+  +A L++ FD +LP G+   ++D+ E  GLT  + + L ++
Sbjct: 441 CPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDVEEAPGLTVNKKNELLLV 492

BLAST of CmaCh16G009180 vs. TAIR10
Match: AT5G57260.1 (AT5G57260.1 cytochrome P450, family 71, subfamily B, polypeptide 10)

HSP 1 Score: 332.8 bits (852), Expect = 3.4e-91
Identity = 172/470 (36.60%), Postives = 278/470 (59.15%), Query Frame = 1

Query: 7   PPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLKAH 66
           PP P G PIIG +H L +L H+ L  LS+ YGP+M +KLG   T+IVS+   A+  LK +
Sbjct: 31  PPSPPGLPIIGNLHQLGELPHQSLCKLSKKYGPVMLLKLGRVPTVIVSTPETAKQVLKDY 90

Query: 67  DPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRREEV 126
           D     RP      +LSY+  +IAF+++  YW+ +RK+C+ +LF   ++NS   ++  E+
Sbjct: 91  DLHCCSRPSLEGTRKLSYNYLDIAFSRFDDYWKELRKLCVEELFCNKRINSIQPIKEAEM 150

Query: 127 GLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFV-----EEEFKASIHEATLL 186
             LID + E+A    +V+LS    S   +VIC+ VFG  F       ++F+  +HEA  +
Sbjct: 151 EKLIDSIAESASQKTLVNLSDTFLSLNVNVICKAVFGVNFQGTVLNNDKFQDLVHEALEM 210

Query: 187 VGAPDLGDFIPFISMLD--LQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFLD 246
           +G+    DF P++  +     GL  R +   +  D F + +I  HL+ N E  ++  F+D
Sbjct: 211 LGSFSASDFFPYVGWIVDWFTGLHARRERSVRDLDAFYEQMIDLHLQKNRE-ESEDDFVD 270

Query: 247 VLLD----------SKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQK 306
           +LL            K+  + IK ++M++++GG+++S  T+ WA++ELI++P  MKKVQ 
Sbjct: 271 LLLRLEKEEAVLGYGKLTRNHIKAILMNILLGGINTSAITMTWAMAELIRNPRVMKKVQS 330

Query: 307 ELENVVGLG---KMVEESDLRELKYLEMVIKETFRMHPPIP-LIPRKCIEDCIVNGYHIP 366
           E+   +G     +++   ++  L YL MVIKET R+HP  P L+PR+ I +  +NGY I 
Sbjct: 331 EIRAQIGKNNKTRIISLDEINHLSYLNMVIKETCRLHPVAPLLVPREVISEFKINGYTIQ 390

Query: 367 KNSEVLINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLG 426
             + + +N WAIGRDP +W DP  F PERF +  +D +G+D++L PFG G R CP + +G
Sbjct: 391 PKTRLHVNVWAIGRDPEIWKDPEEFLPERFMDCDIDVKGQDYELLPFGSGRRICPAVYMG 450

Query: 427 LVLVQLVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVR 456
           +  V+  +A L++ FD +LP G++  ++ M E  GLT  + H+L ++ V+
Sbjct: 451 ITTVEFGLANLLYHFDWKLPEGVAVEDIYMDEASGLTSHKKHDLLLVPVK 499

BLAST of CmaCh16G009180 vs. NCBI nr
Match: gi|778688376|ref|XP_011652735.1| (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis sativus])

HSP 1 Score: 631.3 bits (1627), Expect = 1.3e-177
Identity = 305/465 (65.59%), Postives = 371/465 (79.78%), Query Frame = 1

Query: 4   KKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFL 63
           K LPPGPKGFPIIG++HLLK LVHRDLH LSQ YGPIMHMKLGL+STI+VSS +AA+LFL
Sbjct: 28  KNLPPGPKGFPIIGSLHLLKNLVHRDLHKLSQTYGPIMHMKLGLQSTIVVSSPNAAKLFL 87

Query: 64  KAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRR 123
           K HDP+FA RP+  T NQLSY+ KNIA+AQ+GPYW+ MRKIC  QL T+ K+NSFTS+R 
Sbjct: 88  KTHDPIFANRPISQTSNQLSYNHKNIAYAQFGPYWQTMRKICSFQLLTSSKINSFTSIRM 147

Query: 124 EEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEEFKASIHEATLLVG 183
           +E+G  ID LQEAAK H +VDLS Q+SS IADVIC M+FG+KF ++EF  +IHE T L G
Sbjct: 148 QELGFFIDRLQEAAKNHVIVDLSYQVSSLIADVICIMLFGKKFTDKEFMVAIHEGTFLAG 207

Query: 184 APDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRT-KG-CFLDVL 243
           A +LGDFIPFI+ LDLQGLGRRAK V K+FD  LD+II++ LE   +NRT KG  F+DV+
Sbjct: 208 ASNLGDFIPFIAFLDLQGLGRRAKAVIKIFDELLDVIIEERLEYKKQNRTEKGEFFVDVM 267

Query: 244 LD--------SKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKELEN 303
           LD         +ID SSIK VI+DLIIGGVDSS   I+W LSELIKHP  MKK++KEL+ 
Sbjct: 268 LDVIRSREKEHQIDRSSIKAVILDLIIGGVDSSSAAINWTLSELIKHPQVMKKIRKELQE 327

Query: 304 VVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPLIPRKCIEDCIVNGYHIPKNSEVLIN 363
           VVGL KMVEES L +LKYL+M IKET R+HP +PLIPRK I+DC VNGYHIPKN++++IN
Sbjct: 328 VVGLNKMVEESHLNQLKYLDMTIKETLRIHPSLPLIPRKSIQDCNVNGYHIPKNTDIIIN 387

Query: 364 EWAIGRDPCVWFDPLTFFPERFANRKMD--TRGKDFQLHPFGYGCRNCPGMQLGLVLVQL 423
           +WAIG+DPC W +P  F P+RF +  +D      +F++ PFG+G R CPGMQLGLVL++L
Sbjct: 388 DWAIGQDPCYWIEPQKFNPDRFIDSPIDIVRNNNNFEMIPFGFGRRVCPGMQLGLVLIRL 447

Query: 424 VVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVRR 457
           +VAQLVH FD ELPNG+ PSELDM+E+FGLTC RA NLRV+ + R
Sbjct: 448 IVAQLVHCFDWELPNGVLPSELDMSEEFGLTCPRAQNLRVVPIYR 492

BLAST of CmaCh16G009180 vs. NCBI nr
Match: gi|778688373|ref|XP_011652733.1| (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis sativus])

HSP 1 Score: 625.9 bits (1613), Expect = 5.4e-176
Identity = 304/465 (65.38%), Postives = 373/465 (80.22%), Query Frame = 1

Query: 4   KKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFL 63
           K LPPGPKGFPIIG++HLLK LVHRDLH LSQ YGPIMHMKLGL+STI+VSS +AA+LFL
Sbjct: 28  KNLPPGPKGFPIIGSLHLLKNLVHRDLHKLSQTYGPIMHMKLGLQSTIVVSSPNAAKLFL 87

Query: 64  KAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRR 123
           K HDP+FA RPV  T NQ+SY  KNIAF Q+GPYW++MRKIC   L T+ K+NSF+S+RR
Sbjct: 88  KTHDPIFANRPVPQTSNQMSYDHKNIAFVQFGPYWQSMRKICSSHLLTSSKVNSFSSIRR 147

Query: 124 EEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEEFKASIHEATLLVG 183
           +E+GLLI HL+EAA+ H +VDLSS+ISS   DVIC M+FG+KFV++E  A+I E T L G
Sbjct: 148 QELGLLIHHLKEAARNHAIVDLSSKISSLTFDVICVMLFGKKFVDKELTAAIREGTSLSG 207

Query: 184 APDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKG--CFLDVL 243
           AP+LGDF PFI+ LDLQGLGRRAK V KV DGFLDMII++ LE  D+N+T+    F+DV+
Sbjct: 208 APNLGDFFPFIAFLDLQGLGRRAKAVNKVVDGFLDMIIEERLEFKDKNKTESSELFVDVM 267

Query: 244 LD--------SKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKELEN 303
           LD         +ID S+IK VI DL+IGGVDSS TTI WALSE+IK+P  MKK+Q+EL+ 
Sbjct: 268 LDLIRSEEMEHQIDRSNIKAVIFDLMIGGVDSSSTTIIWALSEIIKNPQVMKKIQEELKE 327

Query: 304 VVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPLIPRKCIEDCIVNGYHIPKNSEVLIN 363
           VVGL KMVEES L +LKYL+M IKE+ R+HP IPLIPRK I+DC VNGYHIPKN++++IN
Sbjct: 328 VVGLNKMVEESHLNQLKYLDMTIKESLRIHPVIPLIPRKSIQDCNVNGYHIPKNTDIIIN 387

Query: 364 EWAIGRDPCVWFDPLTFFPERFANRKMDTRG--KDFQLHPFGYGCRNCPGMQLGLVLVQL 423
           +WAIG+DPC W +P  F P+RF + ++D  G   +F++ PFG G R CPGMQLGLVLV++
Sbjct: 388 DWAIGQDPCYWIEPQKFNPDRFVDTQIDFIGNKNNFEMIPFGSGRRGCPGMQLGLVLVRM 447

Query: 424 VVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVRR 457
           +VAQLVH FD ELPNG+ PSELDM+E FGL+C RA NLRV+ V R
Sbjct: 448 IVAQLVHCFDWELPNGVLPSELDMSEDFGLSCPRAQNLRVVPVYR 492

BLAST of CmaCh16G009180 vs. NCBI nr
Match: gi|659080950|ref|XP_008441066.1| (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo])

HSP 1 Score: 587.8 bits (1514), Expect = 1.6e-164
Identity = 288/467 (61.67%), Postives = 359/467 (76.87%), Query Frame = 1

Query: 5   KLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLK 64
           KLPPGPKGFPIIG++HLL KL+H+DLH+LSQ+YGPIMH++LGL   IIVSS+ A ELFLK
Sbjct: 32  KLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIMHIQLGLLPAIIVSSARATELFLK 91

Query: 65  AHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRRE 124
            HD  FA RP+ +T N +SY +K I FAQYGPYWRN+RK+C L+L ++LK+NSF+SMR++
Sbjct: 92  THDLHFASRPLTITSNHISYGRKGIVFAQYGPYWRNIRKMCTLELLSSLKINSFSSMRKQ 151

Query: 125 EVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEE-----FKASIHEAT 184
           EVG LI  L++AA     VDL+S+ISS I D+IC MV GRK+ + E     FK  I EAT
Sbjct: 152 EVGSLIKGLEDAATDGVAVDLTSKISSVIGDMICVMVLGRKYEDNELGEKGFKGLIREAT 211

Query: 185 LLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFLD 244
            L  AP+LGDFIP I+  D+QG G RAK V K+FDGFL+ I+++H+    +N+ K  F+D
Sbjct: 212 QLAAAPNLGDFIPLIARFDVQGFGGRAKAVGKIFDGFLESIVEEHVVFEKDNKDKD-FVD 271

Query: 245 VLLDS------KIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKELEN 304
           VLLD       +ID S+IK +I+D +I  VDS+ TTI+W LSELIKHPH MKK+Q+ELE 
Sbjct: 272 VLLDLMGSREYQIDRSNIKAIILDFVIAAVDSTTTTINWMLSELIKHPHIMKKLQEELEK 331

Query: 305 VVGLGKMVEESDLRELKYLEMVIKETFRMHPPIPLIPRKCIEDCIVNGYHIPKNSEVLIN 364
           VVGL +MVEESDL  LKYLEMV+KE+ RMHPP+PLIPR+CI+DC +NGYHIPK S ++IN
Sbjct: 332 VVGLNRMVEESDLSNLKYLEMVMKESLRMHPPVPLIPRECIQDCNINGYHIPKKSRIVIN 391

Query: 365 EWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLVLVQLVV 424
            WAIGRDP  W DP  F PERF   ++D RG+DF+L PFG G R C G+QL LV+V+LVV
Sbjct: 392 AWAIGRDPNTWVDPHKFDPERFLESEVDVRGRDFELIPFGSGRRGCVGIQLALVVVRLVV 451

Query: 425 AQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVI-LVRRAPN 460
           AQL+H FDL+LP+G+SP ELDMTE  GLTC RA NLRVI + R  PN
Sbjct: 452 AQLLHCFDLKLPDGMSPLELDMTEILGLTCPRAQNLRVIPIFRLCPN 497

BLAST of CmaCh16G009180 vs. NCBI nr
Match: gi|659080218|ref|XP_008440675.1| (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo])

HSP 1 Score: 558.5 bits (1438), Expect = 1.1e-155
Identity = 271/469 (57.78%), Postives = 351/469 (74.84%), Query Frame = 1

Query: 5   KLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLK 64
           KLPPGP+GFPI G +HLL KL HRDL  LS+ YG IM+M+LGL  T+IVSS  AAELFLK
Sbjct: 27  KLPPGPRGFPIFGCLHLLGKLPHRDLRSLSKKYGSIMYMRLGLVPTVIVSSPQAAELFLK 86

Query: 65  AHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRRE 124
            HD VFA RP       +SY QKN+ FAQYGPYWRNMRK+C L+L +++K+ SF SMR E
Sbjct: 87  THDTVFASRPFVQASKYMSYGQKNLGFAQYGPYWRNMRKMCTLELLSSVKVKSFRSMRME 146

Query: 125 EVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEE-----FKASIHEAT 184
           E+GLL+D+L++AAK   +V+LSS+I S   D+ C MVFG+K+ ++E     FK+ I EA 
Sbjct: 147 ELGLLVDYLRDAAKKRVIVNLSSKICSLNTDMTCLMVFGKKYEDQEFDERGFKSVIQEAM 206

Query: 185 LLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFLD 244
            LV +P+LGDFIP I+ LDLQGL RR+K ++K+FDGFL+ II +HLES +EN+T   F+D
Sbjct: 207 QLVASPNLGDFIPQIAGLDLQGLDRRSKAISKIFDGFLERIIDEHLESKNENKTMD-FVD 266

Query: 245 VLL--------DSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKEL 304
           V+L        + +I+ S+IK +I+D+++  +D+S TTI WA+ ELIKHPH MKK+Q EL
Sbjct: 267 VMLEIMGSQETEYQIERSNIKALILDMLVAAMDTSATTIGWAIPELIKHPHVMKKMQDEL 326

Query: 305 ENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIP-LIPRKCIEDCIVNGYHIPKNSEV 364
           E VVGL + VEE+DL  L+YL+MV+KE  R+HPP P L+P + +EDCIV+G++IPK S +
Sbjct: 327 EKVVGLDRKVEETDLDHLEYLDMVVKEILRLHPPAPLLVPHEALEDCIVDGFYIPKKSRI 386

Query: 365 LINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLVLVQ 424
           ++N WAIGRDP  W DP  FFPERF   ++D RG+DFQL PFG G R CPGMQ+GL +V+
Sbjct: 387 IVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVRGRDFQLIPFGSGRRGCPGMQMGLTVVR 446

Query: 425 LVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVRRAPN 460
           LV+AQLVH FD ELPNG  P +LDMTE+FGLTC RA +L V  + R  N
Sbjct: 447 LVIAQLVHCFDWELPNGTLPVKLDMTEEFGLTCPRAQDLMVTPIYRLHN 494

BLAST of CmaCh16G009180 vs. NCBI nr
Match: gi|778722447|ref|XP_011658487.1| (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis sativus])

HSP 1 Score: 553.9 bits (1426), Expect = 2.6e-154
Identity = 267/469 (56.93%), Postives = 350/469 (74.63%), Query Frame = 1

Query: 5   KLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQLYGPIMHMKLGLKSTIIVSSSHAAELFLK 64
           KLPPGP+GFP+ G +HLL KL HRDL  LS+ YG IM+M+LGL  TIIVSS  AAELFLK
Sbjct: 27  KLPPGPRGFPVFGCLHLLGKLPHRDLQSLSEKYGSIMYMRLGLVPTIIVSSPQAAELFLK 86

Query: 65  AHDPVFAGRPVRLTFNQLSYHQKNIAFAQYGPYWRNMRKICILQLFTTLKMNSFTSMRRE 124
            HD VFA RP       ++Y QKN+AF+QYGPYWRN+RK+C L+L +++K+ SF SMR E
Sbjct: 87  THDSVFASRPFVQASKYMAYGQKNLAFSQYGPYWRNLRKMCTLELLSSVKVKSFRSMRME 146

Query: 125 EVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRMVFGRKFVEEE-----FKASIHEAT 184
           E+GL +D+L++AA+   +V+LSS+I S   D+ C MVFG+K+ ++E     FK+ I EA 
Sbjct: 147 ELGLFVDYLRDAARKRVIVNLSSKICSLNTDMTCLMVFGKKYKDQEFDERGFKSVIQEAM 206

Query: 185 LLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMIIKQHLESNDENRTKGCFLD 244
            +V +P+LGDFIP I++LDLQGL RR+K V+K+FD F + II +HLES  EN+TK  F+D
Sbjct: 207 QIVASPNLGDFIPQIAVLDLQGLDRRSKAVSKIFDEFFERIIDEHLESRYENKTKD-FVD 266

Query: 245 VLL--------DSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALSELIKHPHTMKKVQKEL 304
           V+L        + +I+ S+IK +I+D+++  +D+S TTI WA+ ELIKHPH MKK+Q EL
Sbjct: 267 VMLEIMDSQGTEYQIERSNIKAIILDMLVAAMDTSATTIGWAIPELIKHPHVMKKMQDEL 326

Query: 305 ENVVGLGKMVEESDLRELKYLEMVIKETFRMHPPIP-LIPRKCIEDCIVNGYHIPKNSEV 364
           + VVGL + VEE+DL  L+YL+MV+KE  R+HPP P L+P + +EDCIV+G++IPK S +
Sbjct: 327 QKVVGLDRKVEETDLDHLQYLDMVVKEILRLHPPAPLLVPHEALEDCIVDGFYIPKKSRI 386

Query: 365 LINEWAIGRDPCVWFDPLTFFPERFANRKMDTRGKDFQLHPFGYGCRNCPGMQLGLVLVQ 424
           ++N WAIGRDP  W DP  FFPERF   ++D RGKDFQL PFG G R CPGMQ+GL +V+
Sbjct: 387 IVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVRGKDFQLIPFGSGRRGCPGMQMGLTVVR 446

Query: 425 LVVAQLVHWFDLELPNGISPSELDMTEQFGLTCTRAHNLRVILVRRAPN 460
           LV+AQLVH FD ELPNG  P ELDMTE+FGLTC RA +L V  + R  N
Sbjct: 447 LVIAQLVHCFDWELPNGTLPVELDMTEEFGLTCPRAQDLMVTPIYRLNN 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C7A12_PANGI9.7e-12548.06Cytochrome P450 CYP736A12 OS=Panax ginseng PE=2 SV=1[more]
C71A1_PERAE1.5e-10140.72Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=2[more]
F3PH_ARATH1.9e-9639.96Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1[more]
C84A1_ARATH3.3e-9637.66Cytochrome P450 84A1 OS=Arabidopsis thaliana GN=CYP84A1 PE=1 SV=1[more]
C75A1_PINTA6.8e-9439.22Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KGD3_CUCSA7.4e-14856.14Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501350 PE=3 SV=1[more]
B9HFV3_POPTR9.7e-14856.03Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s06240g PE=3 SV=2[more]
V4SVX2_9ROSI1.8e-14656.16Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027549mg PE=3 SV=1[more]
B9HFW2_POPTR5.3e-14655.72Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s06310g PE=3 SV=2[more]
M5WID8_PRUPE1.2e-14555.82Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004664mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07990.11.1e-9739.96 Cytochrome P450 superfamily protein[more]
AT4G36220.11.8e-9737.66 ferulic acid 5-hydroxylase 1[more]
AT2G45560.14.0e-9237.42 cytochrome P450, family 76, subfamily C, polypeptide 1[more]
AT3G26310.18.8e-9236.29 cytochrome P450, family 71, subfamily B, polypeptide 35[more]
AT5G57260.13.4e-9136.60 cytochrome P450, family 71, subfamily B, polypeptide 10[more]
Match NameE-valueIdentityDescription
gi|778688376|ref|XP_011652735.1|1.3e-17765.59PREDICTED: cytochrome P450 CYP736A12-like [Cucumis sativus][more]
gi|778688373|ref|XP_011652733.1|5.4e-17665.38PREDICTED: cytochrome P450 CYP736A12-like [Cucumis sativus][more]
gi|659080950|ref|XP_008441066.1|1.6e-16461.67PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo][more]
gi|659080218|ref|XP_008440675.1|1.1e-15557.78PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo][more]
gi|778722447|ref|XP_011658487.1|2.6e-15456.93PREDICTED: cytochrome P450 CYP736A12-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
IPR017972Cyt_P450_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G009180.1CmaCh16G009180.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 390..399
score: 4.8E-7coord: 261..278
score: 4.8E-7coord: 314..325
score: 4.
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 4..457
score: 5.2E
IPR001128Cytochrome P450PFAMPF00067p450coord: 7..441
score: 2.9
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 7..456
score: 1.03E
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 152..170
score: 1.2E-41coord: 250..267
score: 1.2E-41coord: 270..296
score: 1.2E-41coord: 313..331
score: 1.2E-41coord: 389..399
score: 1.2E-41coord: 34..53
score: 1.2E-41coord: 399..422
score: 1.2E-41coord: 353..377
score: 1.2E-41coord: 58..79
score: 1.2
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 392..401
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 4..459
score: 6.3E
NoneNo IPR availablePANTHERPTHR24298:SF157SUBFAMILY NOT NAMEDcoord: 4..459
score: 6.3E

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh16G009180Cucurbita maxima (Rimu)cmacmaB358
CmaCh16G009180Cucurbita moschata (Rifu)cmacmoB345
CmaCh16G009180Watermelon (Charleston Gray)cmawcgB285
CmaCh16G009180Watermelon (97103) v1cmawmB300
CmaCh16G009180Cucurbita pepo (Zucchini)cmacpeB345
CmaCh16G009180Cucumber (Gy14) v2cgybcmaB340
CmaCh16G009180Silver-seed gourdcarcmaB0676
CmaCh16G009180Cucumber (Chinese Long) v3cmacucB0381
CmaCh16G009180Watermelon (97103) v2cmawmbB338
CmaCh16G009180Wax gourdcmawgoB0419