CmaCh16G008200 (gene) Cucurbita maxima (Rimu)

NameCmaCh16G008200
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionTerpene synthase 4-2
LocationCma_Chr16 : 4561241 .. 4562533 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCTTGTCCGGTAAGGAAATAAAAATAAGTAGTGTATTAGACTGGATGTATACTTTGAACTGCAGTTCAATGCCGAAAGAAAAATATTAATCGAAATAATCGTCATTTCACATATGAGGGTGCACGGAGAAGACTCAACCCCAAATTTGTTGCTCAAGTTGGCCGTGCTTTGAAATAGCCACTAAGAAAAGCCCTTCCAAGATTAAAGGCAAGAGAGTACATATCAATGTATTAATAAGGTCCTTTCCATTCTCAAACTTTGCTTACTTTTTCAAAACTGAATTTCAACATAAACTTCATCAAAATGAGGGGAAGGAACTCACCATGTTTATATTTATTTGTGTATGCAATTTCATATTTAACCTATAAAGTTACATTTTTAGGTTCATTCCTACTATTTATTTTAAATTATATGTTATTTCTTCTTATTTACATATTTTATATTTTGTAGGTGGTAGAAAGATTTGAATATTCAAACAAATTTCTCTGATGCAAGAGATAGAATTGTTGAGTGCTAGTTTGTGACATTGAGTGCATACTTTGAACTGCAGTTTAATGTCGAAAGAAAAATATTAACTAAAATAATAGCTATTTCTTCAATTTTAGATGAGACGTATGATGCATATGAAACATTTGAAGAACTCCAATTTTTCACTCGAGCAATTCAAAGGTCATTTGAATTCACTCCTATAGATAACTAAGAAATTTATTGTTTACATATAGCATACCCTTTAAATATACCATACAAATTTCTAAAGTTGAGAAATAGAAGAAAATATTTTTGAAATTTTGTCTTTTTAATATTTCAAATTTGGATAGAATTTTAATACTATTAATTTCAAATTTGGAGAGAAGAGTAATACAAAACACTTTTTTCTTTCAAAATTTATATACGAGAATCATATGAACAATAATGTTTTTGAATTTTCAAATGGAATAGAAGCATAGTTGATATACTCCCGAAGTACATGAAACTTTTATATATAGCAATGTTAAATCTTTATAAAGAAATCAGCAATGAAATAGGTAAGGATGAAAGTTCGTTCACATTCATACCACCAAAGAAGAAGTAAGCTTTCTTTGTCTCCCTCCTCTATCTTCTTTCCTTCTGTTTTATACATATAAATAAGAGTTATCATTTTGGTTTATATTTACAGATAAAAAAGCTAGCTAGATCCTACTTTGAAGAAGCTAAATGGTTAAACAAAAATTACAAACCAAAATTGAAAGAGTACAGAGAATGGGTATTGCATAGCTCAGGATATTCCTTGATTGTAAACATTTCATAG

mRNA sequence

ATGAATCTTGTCCGTTGGCCGTGCTTTGAAATAGCCACTAAGAAAAGCCCTTCCAAGATTAAAGGCAAGAGAGTACATATCAATGTATTAATAAGGTCCTTTCCATTCTCAAACTTTGCTTACTTTTTCAAAACTGAATTTCAACATAAACTTCATCAAAATGAGGGGAAGGAACTCACCATGTTTATATTTATTTGTAAAGATTTGAATATTCAAACAAATTTCTCTGATGCAAGAGATAGAATTTTTAATGTCGAAAGAAAAATATTAACTAAAATAATAGCTATTTCTTCAATTTTAGATGAGACGTATGATGCATATGAAACATTTGAAGAACTCCAATTTTTCACTCGAGCAATTCAAAGAAGCATAGTTGATATACTCCCGAAGTACATGAAACTTTTATATATAGCAATGTTAAATCTTTATAAAGAAATCAGCAATGAAATAGGTAAGGATGAAAGTTCGTTCACATTCATACCACCAAAGAAGAAAGTTATCATTTTGATAAAAAAGCTAGCTAGATCCTACTTTGAAGAAGCTAAATGGTTAAACAAAAATTACAAACCAAAATTGAAAGAGTACAGAGAATGGGTATTGCATAGCTCAGGATATTCCTTGATTGTAAACATTTCATAG

Coding sequence (CDS)

ATGAATCTTGTCCGTTGGCCGTGCTTTGAAATAGCCACTAAGAAAAGCCCTTCCAAGATTAAAGGCAAGAGAGTACATATCAATGTATTAATAAGGTCCTTTCCATTCTCAAACTTTGCTTACTTTTTCAAAACTGAATTTCAACATAAACTTCATCAAAATGAGGGGAAGGAACTCACCATGTTTATATTTATTTGTAAAGATTTGAATATTCAAACAAATTTCTCTGATGCAAGAGATAGAATTTTTAATGTCGAAAGAAAAATATTAACTAAAATAATAGCTATTTCTTCAATTTTAGATGAGACGTATGATGCATATGAAACATTTGAAGAACTCCAATTTTTCACTCGAGCAATTCAAAGAAGCATAGTTGATATACTCCCGAAGTACATGAAACTTTTATATATAGCAATGTTAAATCTTTATAAAGAAATCAGCAATGAAATAGGTAAGGATGAAAGTTCGTTCACATTCATACCACCAAAGAAGAAAGTTATCATTTTGATAAAAAAGCTAGCTAGATCCTACTTTGAAGAAGCTAAATGGTTAAACAAAAATTACAAACCAAAATTGAAAGAGTACAGAGAATGGGTATTGCATAGCTCAGGATATTCCTTGATTGTAAACATTTCATAG

Protein sequence

MNLVRWPCFEIATKKSPSKIKGKRVHINVLIRSFPFSNFAYFFKTEFQHKLHQNEGKELTMFIFICKDLNIQTNFSDARDRIFNVERKILTKIIAISSILDETYDAYETFEELQFFTRAIQRSIVDILPKYMKLLYIAMLNLYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLHSSGYSLIVNIS
BLAST of CmaCh16G008200 vs. Swiss-Prot
Match: GCSY1_CUCME ((+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.3e-36
Identity = 94/191 (49.21%), Postives = 120/191 (62.83%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    KDL++ T F  ARDRI               ++
Sbjct: 250 FSKLDFNILQKLHQKELSEICRW---WKDLDVPTKFPFARDRIVECYFWTLGAYFEPQYS 309

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
           V RK+LTK+IAI+SILD+ YDAY TFEELQ  T AIQR   S+V  LP YMK  Y+AML 
Sbjct: 310 VGRKMLTKVIAIASILDDIYDAYGTFEELQVLTPAIQRWDRSMVHTLPLYMKPFYVAMLE 369

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           LY+EI  EI KD++S       +  I  IK+L+ SYFEEAKWLNK YKP  KEY E  L 
Sbjct: 370 LYEEIGKEIDKDQNSLHL----QVAIGGIKRLSESYFEEAKWLNKEYKPSFKEYMELALK 429

BLAST of CmaCh16G008200 vs. Swiss-Prot
Match: TPSGD_VITVI ((-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.9e-23
Identity = 67/181 (37.02%), Postives = 100/181 (55.25%), Query Frame = 1

Query: 50  KLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FNVERKILTKII 109
           KLHQ E  +++ +    KDL+       ARDR+               F   R+ILTK+I
Sbjct: 247 KLHQKELSDISAW---WKDLDFAHKLPFARDRVVECYFWILGVYFEPQFFFARRILTKVI 306

Query: 110 AISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLNLYKEISNEIG 169
           A++SI+D+ YD Y T EEL+ FT A++R   S +D LP+YM++ Y A+L +Y EI  E+ 
Sbjct: 307 AMTSIIDDIYDVYGTLEELELFTEAVERWDISAIDQLPEYMRVCYQALLYVYSEIEEEMA 366

Query: 170 KDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLHSSGYSLIVNI 213
           K+  S+     K+     +K   R+Y+EEAKWL     P ++EY    L +S YS++   
Sbjct: 367 KEGRSYRLYYAKE----AMKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATT 420

BLAST of CmaCh16G008200 vs. Swiss-Prot
Match: TPS1_PHYDL (Bifunctional sesquiterpene synthase 1 OS=Phyla dulcis PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.9e-23
Identity = 73/177 (41.24%), Postives = 98/177 (55.37%), Query Frame = 1

Query: 51  LHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FNVERKILTKIIA 110
           LH+ E  E++ +    K ++I  NF  ARDRI               + V RKI TK+IA
Sbjct: 255 LHKVELSEISRW---WKGVDIARNFPYARDRIVELYFWVLGVYFEPQYAVGRKITTKVIA 314

Query: 111 ISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLNLYKEISNEIGK 170
           I+S+LD+T+DAY TFEEL+ F  A++R   S +D LP+YMKLLY  ML +  EI  E+ K
Sbjct: 315 IASLLDDTFDAYGTFEELRIFAEAVERWSVSCLDQLPEYMKLLYKTMLEVSDEIEEEMTK 374

Query: 171 DESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLHSSGYSLIV 210
             + F         I  IK  ARSYF EAKW  + YKP  +EY      + GY  ++
Sbjct: 375 LGTPFRI----AYGIEAIKTFARSYFLEAKWREEKYKPTTEEYMGLATKTCGYKSLI 424

BLAST of CmaCh16G008200 vs. Swiss-Prot
Match: TPS6_PHYDL (Beta-caryophyllene synthase OS=Phyla dulcis PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 8.4e-23
Identity = 67/180 (37.22%), Postives = 103/180 (57.22%), Query Frame = 1

Query: 47  FQHKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FNVERKILT 106
           F  KLHQ E  +LT +    K+L+ +T    ARDR+               +N+ R  L+
Sbjct: 247 FSQKLHQKEISDLTRW---WKELDFKTKLPFARDRMVECYCWTLGIHPEPQYNLARNFLS 306

Query: 107 KIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLNLYKEISN 166
           K+I ++S++D+ YD   T +ELQ FT AIQR   S ++ LP YM++ Y A+LN+Y E+  
Sbjct: 307 KVIMLASVIDDIYDVRGTLDELQLFTDAIQRWDISAMEQLPPYMRVCYEALLNVYAEVE- 366

Query: 167 EIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLHSSGYSLI 209
           E+ + +  +     K+++    KKLAR+Y EE++WL K Y P  KEY    + SSGY ++
Sbjct: 367 ELERIDGPYRVHYAKEEM----KKLARAYLEESQWLYKKYIPTFKEYMSVAIPSSGYIMV 418

BLAST of CmaCh16G008200 vs. Swiss-Prot
Match: TPSVS_VITVI (Valencene synthase OS=Vitis vinifera GN=ValCS PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-22
Identity = 69/180 (38.33%), Postives = 104/180 (57.78%), Query Frame = 1

Query: 51  LHQNEGKELTMFIFICKDLNIQTNFSDARDRI-----------FNVE----RKILTKIIA 110
           LH+ E   L  +    K+L+  T    ARDR+           F  +    R+ILTK+IA
Sbjct: 248 LHKEELSNLARW---WKELDFATKLPFARDRLVEGYFWMHGVYFEPQYLRGRRILTKVIA 307

Query: 111 ISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLNLYKEISNEIGK 170
           ++SILD+ +DAY T EEL+ F  AI+R   + ++ LP+YMKL Y+A+L++YKEI  E+ K
Sbjct: 308 MTSILDDIHDAYGTPEELKLFIEAIERWDINSINQLPEYMKLCYVALLDVYKEIEEEMEK 367

Query: 171 DESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLHSSGYSLIVNIS 213
           + + +     K+    ++K   R+YF EAKWL++ + P  +EY    L SSGY L+   S
Sbjct: 368 EGNQYRVHYAKE----VMKNQVRAYFAEAKWLHEEHVPAFEEYMRVALASSGYCLLATTS 420

BLAST of CmaCh16G008200 vs. TrEMBL
Match: A0A0A0L2I8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.7e-36
Identity = 97/191 (50.79%), Postives = 122/191 (63.87%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    KDL++ T F  ARDRI               +N
Sbjct: 237 FSKLDFNILQKLHQKELSEICRW---WKDLDVPTKFPFARDRIVECYFWTLGAYFEPQYN 296

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
           V RK+LTK+IAISSILD+ YDAY TFEELQ    AIQR   S+V  LP+YMK  Y+AM  
Sbjct: 297 VGRKMLTKVIAISSILDDIYDAYGTFEELQVLAPAIQRWDRSMVHTLPEYMKPFYVAMFE 356

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           +Y+EIS EIGKD++S       +  I  IKKL+ SYFEEAKWLNK YKP  KEY E  L+
Sbjct: 357 VYEEISKEIGKDQNSLHL----QVAIGGIKKLSESYFEEAKWLNKKYKPSFKEYMELALN 416

BLAST of CmaCh16G008200 vs. TrEMBL
Match: A0A0B4VEV4_CUCME (Sesquiterpene synthase Tps1 OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.1e-34
Identity = 93/191 (48.69%), Postives = 122/191 (63.87%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    KDLN+ TNF  ARD+I               F 
Sbjct: 250 FSKLDFNALQKLHQKELAEICRW---WKDLNVSTNFPFARDQIVECYFWILSIYFEPRFK 309

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
             RKILTK+IA++SI+D+ YDAY TFEELQ FT AI+R   S++++LPKYMK+ Y  +L+
Sbjct: 310 FGRKILTKVIAMTSIMDDIYDAYGTFEELQVFTLAIKRWDMSMINLLPKYMKVYYTTLLD 369

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           L++EI  EI  DE+S+ F   K+ +    K+ A SYF+EA+WLNKNYKPK +EY E  L 
Sbjct: 370 LFEEIDKEIVNDETSYRFHFAKEAM----KRQAESYFKEAEWLNKNYKPKYEEYMEVALA 429

BLAST of CmaCh16G008200 vs. TrEMBL
Match: A0A0A0L579_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097040 PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.6e-31
Identity = 90/191 (47.12%), Postives = 117/191 (61.26%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    KDLN+ TNF  ARDRI               F 
Sbjct: 247 FSKLDFNILQKLHQKEIAEICRW---WKDLNVSTNFPFARDRIVECYFWILSIYFEPYFK 306

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
             RKILTK+IA++SI+D+ YDAY TFEELQ FT AI+R   S+V++LP+YMK+ Y  +L+
Sbjct: 307 FGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRWDMSMVNLLPQYMKVHYTTLLD 366

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           L++E+   I  D  S+     K+ +    K+ A SYF+EA+WLNKNYKPK  EY E  L 
Sbjct: 367 LFEEMDKGIVNDGISYRSCFAKEAM----KRQAESYFKEAEWLNKNYKPKYGEYMEVALA 426

BLAST of CmaCh16G008200 vs. TrEMBL
Match: Q66PX8_CUCSA (Beta-caryophyllene synthase OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 3.4e-31
Identity = 90/191 (47.12%), Postives = 117/191 (61.26%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    KDLN+ TNF  ARDRI               F 
Sbjct: 247 FSKLDFNILQKLHQKEIAEICRW---WKDLNVSTNFPFARDRIVECYFWILSIYFEPYFK 306

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
             RKILTK+IA++SI+D+ YDAY TFEELQ FT AI+R   S+V++LP+YMK+ Y  +L+
Sbjct: 307 FGRKILTKVIAMTSIMDDIYDAYGTFEELQLFTLAIKRWDMSMVNLLPQYMKVHYTTLLD 366

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           L++E+   I  D  S+     K+ +    K+ A SYF+EA+WLNKNYKPK  EY E  L 
Sbjct: 367 LFEEMDKGIVNDGISYRSCFGKEAM----KRQAESYFKEAEWLNKNYKPKYGEYMEVALA 426

BLAST of CmaCh16G008200 vs. TrEMBL
Match: U3MLM5_COPLA (Terpene synthase 4-2 OS=Copaifera langsdorffii GN=TPS4-2 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.1e-24
Identity = 72/191 (37.70%), Postives = 109/191 (57.07%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K++F    KLHQ E   L+ +    KDL+  T    ARDR+               ++
Sbjct: 237 FAKSDFNGVQKLHQREIGNLSKW---WKDLDFATKLPFARDRLVEAYFWILGVYFEPCYS 296

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
           + R+I+TK+I+++SI+D+ YD Y T EELQ FT AI +   S +D LP+YMKL+Y  +L+
Sbjct: 297 LARRIMTKVISLTSIIDDIYDVYGTLEELQLFTEAINKWDISCMDFLPEYMKLIYQPLLD 356

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           +Y EI  E  K+  +F     K++    ++K+ R+Y  EAKW + NY P  +EY E  L 
Sbjct: 357 VYDEIERETAKEGRAFCVNYGKEQ----MRKVVRAYLAEAKWFHNNYTPTFEEYMEVALV 416

BLAST of CmaCh16G008200 vs. TAIR10
Match: AT4G16740.1 (AT4G16740.1 terpene synthase 03)

HSP 1 Score: 72.0 bits (175), Expect = 4.9e-13
Identity = 54/204 (26.47%), Postives = 96/204 (47.06%), Query Frame = 1

Query: 19  KIKGKRVHINVLIRSFPFSNFAYFFKTEFQHKLHQNEGKELTMFIFICKDLNIQTNFSDA 78
           ++ G+R  +N ++      +F       F   +HQ+E K L+ +        +  +    
Sbjct: 232 EVYGERHDMNPILLELAKLDF------NFVQAIHQDELKSLSSW---WSKTGLTKHLDFV 291

Query: 79  RDRI---------------FNVERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR- 138
           RDRI               F   R++LTK+  + + +D+ YD Y T EELQ FT  +++ 
Sbjct: 292 RDRITEGYFSSVGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKW 351

Query: 139 --SIVDILPKYMKLLYIAMLNLYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEE 198
             + ++ LP YMKL ++ ++N   +I   + +D+  F  IP  K+       +  ++ +E
Sbjct: 352 DVNRLEELPNYMKLCFLCLVNEINQIGYFVLRDK-GFNVIPYLKE---SWADMCTTFLKE 411

Query: 199 AKWLNKNYKPKLKEYRE--WVLHS 203
           AKW    YKP  +EY +  W+  S
Sbjct: 412 AKWYKSGYKPNFEEYMQNGWISSS 422

BLAST of CmaCh16G008200 vs. TAIR10
Match: AT5G23960.1 (AT5G23960.1 terpene synthase 21)

HSP 1 Score: 71.6 bits (174), Expect = 6.4e-13
Identity = 43/132 (32.58%), Postives = 68/132 (51.52%), Query Frame = 1

Query: 83  FNVERKILTKIIAISSILDETYDAYETFEELQFFTRAIQRSIVDI-----LPKYMKLLYI 142
           ++  R I T  + + + LD+ YDAY T EEL+ FT A+   +  +     +P  MK +Y 
Sbjct: 281 YSQARVITTMALILFTALDDMYDAYGTMEELELFTDAMDEWLPVVPDEIPIPDSMKFIYN 340

Query: 143 AMLNLYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYRE 202
             +  Y ++  E+ K+  S      KK     ++K A  Y +EAKWL K+Y     EY+E
Sbjct: 341 VTVEFYDKLDEELEKEGRSGCGFHLKKS----LQKTANGYMQEAKWLKKDYIATFDEYKE 400

Query: 203 WVLHSSGYSLIV 210
             + SSGY  ++
Sbjct: 401 NAILSSGYYALI 408

BLAST of CmaCh16G008200 vs. TAIR10
Match: AT4G16730.1 (AT4G16730.1 terpene synthase 02)

HSP 1 Score: 70.9 bits (172), Expect = 1.1e-12
Identity = 61/210 (29.05%), Postives = 97/210 (46.19%), Query Frame = 1

Query: 24  RVHINVLIRSFPFSNFAYFFKTEFQHKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI- 83
           R +INV  +    +     F     + L  N  +EL +         +       RDRI 
Sbjct: 197 RWYINVYEKKHEMNPLLLEFAAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRIT 256

Query: 84  --------------FNVERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIV 143
                         F   RKILTKI  +  I+D+ YD Y T EEL+ FT  +++   + V
Sbjct: 257 ESYFWTIGIFYEPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKWDVNHV 316

Query: 144 DILPKYMKLLYIAMLNLYKEISNEIGKD---ESSFTFIPPKKKVIILIKKLARSYFEEAK 203
           + LP YM++ +   L LY EI N+IG D   +     IP  K+V      L +++  E+K
Sbjct: 317 ERLPNYMRMCF---LFLYNEI-NQIGYDVLRDKGLNVIPYLKQV---WTDLFKTFLTESK 376

Query: 204 WLNKNYKPKLKEY-REWVLHSSGYSLIVNI 212
           W    +KP  +EY +  V+ SS  ++++++
Sbjct: 377 WYKTGHKPSFEEYMQNGVISSSVPTILLHL 399

BLAST of CmaCh16G008200 vs. TAIR10
Match: AT1G70080.1 (AT1G70080.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 70.9 bits (172), Expect = 1.1e-12
Identity = 38/118 (32.20%), Postives = 68/118 (57.63%), Query Frame = 1

Query: 83  FNVERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAM 142
           ++  R +L K I +  I+D T+D Y T +E++   +AI+R     VD+LP Y+K+++   
Sbjct: 345 YSAARIMLAKSIILVDIVDNTFDVYGTIDEVKSLVQAIERWDSDAVDVLPDYLKVVFRTT 404

Query: 143 LNLYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYRE 198
            +L+KE+   +  +  SFT     +++ IL+K     Y +EA+W N+ + P  +EY E
Sbjct: 405 FDLFKELEEYVSSEARSFTMQYAYEQLRILMK----GYLQEAEWSNRGHLPSHEEYIE 458

BLAST of CmaCh16G008200 vs. TAIR10
Match: AT3G25830.1 (AT3G25830.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 68.9 bits (167), Expect = 4.2e-12
Identity = 51/135 (37.78%), Postives = 74/135 (54.81%), Query Frame = 1

Query: 83  FNVERKILTKIIAISSILDETYDAYETFEELQFFTRAIQRSIVDIL---PKYMKLLYIAM 142
           F   R+I+T + A+ + +D+ YD Y T EEL+ FT  ++   V+ L   P+YM+L ++  
Sbjct: 324 FGYIRRIMTIVNALVTTIDDIYDIYGTLEELELFTSMVENWDVNRLGELPEYMRLCFLI- 383

Query: 143 LNLYKEISNEIGKDESSFTFIPPKKKVIILIKK----LARSYFEEAKWLNKNYKPKLKEY 202
             LY EI N IG D   +     K  VI  +KK    L R+Y  EAKW  + YKP L+EY
Sbjct: 384 --LYNEI-NGIGCDILKYK----KIDVIPYLKKSWADLCRTYLVEAKWYKRGYKPSLEEY 443

Query: 203 RE--WVLHSSGYSLI 209
            +  W+  S+   LI
Sbjct: 444 MQNAWISISAPTILI 450

BLAST of CmaCh16G008200 vs. NCBI nr
Match: gi|778676208|ref|XP_011650545.1| (PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus])

HSP 1 Score: 160.2 bits (404), Expect = 3.9e-36
Identity = 97/191 (50.79%), Postives = 122/191 (63.87%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    KDL++ T F  ARDRI               +N
Sbjct: 237 FSKLDFNILQKLHQKELSEICRW---WKDLDVPTKFPFARDRIVECYFWTLGAYFEPQYN 296

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
           V RK+LTK+IAISSILD+ YDAY TFEELQ    AIQR   S+V  LP+YMK  Y+AM  
Sbjct: 297 VGRKMLTKVIAISSILDDIYDAYGTFEELQVLAPAIQRWDRSMVHTLPEYMKPFYVAMFE 356

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           +Y+EIS EIGKD++S       +  I  IKKL+ SYFEEAKWLNK YKP  KEY E  L+
Sbjct: 357 VYEEISKEIGKDQNSLHL----QVAIGGIKKLSESYFEEAKWLNKKYKPSFKEYMELALN 416

BLAST of CmaCh16G008200 vs. NCBI nr
Match: gi|745672488|gb|AJD19682.1| (sesquiterpene synthase Tps1 [Cucumis melo])

HSP 1 Score: 154.8 bits (390), Expect = 1.6e-34
Identity = 93/191 (48.69%), Postives = 122/191 (63.87%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    KDLN+ TNF  ARD+I               F 
Sbjct: 250 FSKLDFNALQKLHQKELAEICRW---WKDLNVSTNFPFARDQIVECYFWILSIYFEPRFK 309

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
             RKILTK+IA++SI+D+ YDAY TFEELQ FT AI+R   S++++LPKYMK+ Y  +L+
Sbjct: 310 FGRKILTKVIAMTSIMDDIYDAYGTFEELQVFTLAIKRWDMSMINLLPKYMKVYYTTLLD 369

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           L++EI  EI  DE+S+ F   K+ +    K+ A SYF+EA+WLNKNYKPK +EY E  L 
Sbjct: 370 LFEEIDKEIVNDETSYRFHFAKEAM----KRQAESYFKEAEWLNKNYKPKYEEYMEVALA 429

BLAST of CmaCh16G008200 vs. NCBI nr
Match: gi|661902982|ref|NP_001284382.1| ((+)-gamma-cadinene synthase [Cucumis melo])

HSP 1 Score: 153.7 bits (387), Expect = 3.6e-34
Identity = 94/191 (49.21%), Postives = 120/191 (62.83%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    KDL++ T F  ARDRI               ++
Sbjct: 250 FSKLDFNILQKLHQKELSEICRW---WKDLDVPTKFPFARDRIVECYFWTLGAYFEPQYS 309

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
           V RK+LTK+IAI+SILD+ YDAY TFEELQ  T AIQR   S+V  LP YMK  Y+AML 
Sbjct: 310 VGRKMLTKVIAIASILDDIYDAYGTFEELQVLTPAIQRWDRSMVHTLPLYMKPFYVAMLE 369

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           LY+EI  EI KD++S       +  I  IK+L+ SYFEEAKWLNK YKP  KEY E  L 
Sbjct: 370 LYEEIGKEIDKDQNSLHL----QVAIGGIKRLSESYFEEAKWLNKEYKPSFKEYMELALK 429

BLAST of CmaCh16G008200 vs. NCBI nr
Match: gi|659102820|ref|XP_008452330.1| (PREDICTED: (+)-gamma-cadinene synthase-like [Cucumis melo])

HSP 1 Score: 153.3 bits (386), Expect = 4.8e-34
Identity = 93/191 (48.69%), Postives = 121/191 (63.35%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    KDLN+ TNF  ARDRI               F 
Sbjct: 250 FSKLDFNTLQKLHQKELAEICRW---WKDLNVSTNFPFARDRIVECYFWILSIYFEPHFK 309

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
             RKILTK+IA++SI+D+ YDAY TFEELQ FT AI+R   S++++LPKYMK+ Y  +L+
Sbjct: 310 FGRKILTKVIAMTSIMDDIYDAYGTFEELQVFTLAIKRWDMSMINLLPKYMKVYYTTLLD 369

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           L++EI  EI  DE+S+     K+ +    K+ A SYF+EA+WLNKNYKPK +EY E  L 
Sbjct: 370 LFEEIDKEIVNDETSYRSHFAKEAM----KRQAESYFKEAEWLNKNYKPKYEEYMEVALA 429

BLAST of CmaCh16G008200 vs. NCBI nr
Match: gi|659102883|ref|XP_008452366.1| (PREDICTED: LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like [Cucumis melo])

HSP 1 Score: 144.4 bits (363), Expect = 2.2e-31
Identity = 89/191 (46.60%), Postives = 119/191 (62.30%), Query Frame = 1

Query: 42  FFKTEFQ--HKLHQNEGKELTMFIFICKDLNIQTNFSDARDRI---------------FN 101
           F K +F    KLHQ E  E+  +    +DLN+ TNFS ARDRI               F 
Sbjct: 101 FSKLDFNVLQKLHQTELAEICRW---WEDLNVPTNFSFARDRIVECYFWILSIYFEPHFK 160

Query: 102 VERKILTKIIAISSILDETYDAYETFEELQFFTRAIQR---SIVDILPKYMKLLYIAMLN 161
             RKI TK++A++SI+D+ YDAY  FEELQ FT AI+R   S+V+ILP+ MK+ Y  +L+
Sbjct: 161 FGRKIFTKVVAMTSIMDDIYDAYGIFEELQVFTLAIKRWDMSMVNILPECMKVYYTTLLD 220

Query: 162 LYKEISNEIGKDESSFTFIPPKKKVIILIKKLARSYFEEAKWLNKNYKPKLKEYREWVLH 213
           L++EI  E  KD +S+    P +    ++K+ A SYF+EA+WLNKNYKPK +EY E  L 
Sbjct: 221 LFEEIDKERVKDGTSYRSYFPNE----VMKRQAESYFKEAEWLNKNYKPKYEEYMEVALA 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GCSY1_CUCME2.3e-3649.21(+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1[more]
TPSGD_VITVI2.9e-2337.02(-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1[more]
TPS1_PHYDL2.9e-2341.24Bifunctional sesquiterpene synthase 1 OS=Phyla dulcis PE=1 SV=1[more]
TPS6_PHYDL8.4e-2337.22Beta-caryophyllene synthase OS=Phyla dulcis PE=1 SV=1[more]
TPSVS_VITVI1.4e-2238.33Valencene synthase OS=Vitis vinifera GN=ValCS PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2I8_CUCSA2.7e-3650.79Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1[more]
A0A0B4VEV4_CUCME1.1e-3448.69Sesquiterpene synthase Tps1 OS=Cucumis melo PE=2 SV=1[more]
A0A0A0L579_CUCSA2.6e-3147.12Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097040 PE=3 SV=1[more]
Q66PX8_CUCSA3.4e-3147.12Beta-caryophyllene synthase OS=Cucumis sativus PE=2 SV=1[more]
U3MLM5_COPLA4.1e-2437.70Terpene synthase 4-2 OS=Copaifera langsdorffii GN=TPS4-2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G16740.14.9e-1326.47 terpene synthase 03[more]
AT5G23960.16.4e-1332.58 terpene synthase 21[more]
AT4G16730.11.1e-1229.05 terpene synthase 02[more]
AT1G70080.11.1e-1232.20 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT3G25830.14.2e-1237.78 terpene synthase-like sequence-1,8-cineole[more]
Match NameE-valueIdentityDescription
gi|778676208|ref|XP_011650545.1|3.9e-3650.79PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus][more]
gi|745672488|gb|AJD19682.1|1.6e-3448.69sesquiterpene synthase Tps1 [Cucumis melo][more]
gi|661902982|ref|NP_001284382.1|3.6e-3449.21(+)-gamma-cadinene synthase [Cucumis melo][more]
gi|659102820|ref|XP_008452330.1|4.8e-3448.69PREDICTED: (+)-gamma-cadinene synthase-like [Cucumis melo][more]
gi|659102883|ref|XP_008452366.1|2.2e-3146.60PREDICTED: LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005630Terpene_synthase_metal-bd
IPR008949Isoprenoid_synthase_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0000287magnesium ion binding
GO:0010333terpene synthase activity
GO:0016829lyase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045338 farnesyl diphosphate metabolic process
biological_process GO:0051762 sesquiterpene biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0010334 sesquiterpene synthase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0010333 terpene synthase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G008200.1CmaCh16G008200.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 86..210
score: 2.6
IPR008949Isoprenoid synthase domainGENE3DG3DSA:1.10.600.10coord: 50..211
score: 9.1
IPR008949Isoprenoid synthase domainunknownSSF48576Terpenoid synthasescoord: 49..209
score: 9.21
NoneNo IPR availablePANTHERPTHR31225FAMILY NOT NAMEDcoord: 50..212
score: 4.3
NoneNo IPR availablePANTHERPTHR31225:SF15ALPHA-HUMULENE/(-)-(E)-BETA-CARYOPHYLLENE SYNTHASEcoord: 50..212
score: 4.3

The following gene(s) are paralogous to this gene:

None