CmaCh16G003210 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh16G003210
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Trypsin inhibitor 2
Location	Cma_Chr16 : 1513729 .. 1513992 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTAGCAATAAGATTGTCACGGTGGCTCTAATGGCACTGATGCTGGTAGCGTCGTCTTCGGCTGCCTATGCCATCGACGACGACTCGATGGACCTCGTCTTCGACCGTCGAGACATCAATCATGGAGTCCCAAGAAAGATCATGAAATGGGGAGTGCTTAATCATGAAGAAAGGGTATGTCCCAGAATCCTCATGGAATGCAAAAAGGACTCTGATTGCTTAGCTGAGTGTGTCTGTCTCGAGCATGGATATTGTGGCTAA

mRNA sequence

ATGGGTAGCAATAAGATTGTCACGGTGGCTCTAATGGCACTGATGCTGGTAGCGTCGTCTTCGGCTGCCTATGCCATCGACGACGACTCGATGGACCTCGTCTTCGACCGTCGAGACATCAATCATGGAGTCCCAAGAAAGATCATGAAATGGGGAGTGCTTAATCATGAAGAAAGGGTATGTCCCAGAATCCTCATGGAATGCAAAAAGGACTCTGATTGCTTAGCTGAGTGTGTCTGTCTCGAGCATGGATATTGTGGCTAA

Coding sequence (CDS)

ATGGGTAGCAATAAGATTGTCACGGTGGCTCTAATGGCACTGATGCTGGTAGCGTCGTCTTCGGCTGCCTATGCCATCGACGACGACTCGATGGACCTCGTCTTCGACCGTCGAGACATCAATCATGGAGTCCCAAGAAAGATCATGAAATGGGGAGTGCTTAATCATGAAGAAAGGGTATGTCCCAGAATCCTCATGGAATGCAAAAAGGACTCTGATTGCTTAGCTGAGTGTGTCTGTCTCGAGCATGGATATTGTGGCTAA

Protein sequence

MGSNKIVTVALMALMLVASSSAAYAIDDDSMDLVFDRRDINHGVPRKIMKWGVLNHEERVCPRILMECKKDSDCLAECVCLEHGYCG

Homology

[Close]

BLAST of CmaCh16G003210 vs. Swiss-Prot
Match: ITR3_CUCPE (Trypsin inhibitor 3 OS=Cucurbita pepo PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.5e-13
Identity = 30/32 (93.75%), Postives = 32/32 (100.00%), Query Frame = 1


Query: 56 HEERVCPRILMECKKDSDCLAECVCLEHGYCG 88
          HEERVCP+ILMECKKDSDCLAEC+CLEHGYCG
Sbjct: 1  HEERVCPKILMECKKDSDCLAECICLEHGYCG 32

[Close]

BLAST of CmaCh16G003210 vs. Swiss-Prot
Match: ITR4_CUCMA (Trypsin inhibitor 4 OS=Cucurbita maxima PE=1 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 2.5e-13
Identity = 31/32 (96.88%), Postives = 32/32 (100.00%), Query Frame = 1


Query: 56 HEERVCPRILMECKKDSDCLAECVCLEHGYCG 88
          HEERVCPRILM+CKKDSDCLAECVCLEHGYCG
Sbjct: 1  HEERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of CmaCh16G003210 vs. Swiss-Prot
Match: ITR1_CUCMA (Trypsin inhibitor 1 OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.0e-11
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 1


Query: 59 RVCPRILMECKKDSDCLAECVCLEHGYCG 88
          RVCPRILMECKKDSDCLAECVCLEHGYCG
Sbjct: 1  RVCPRILMECKKDSDCLAECVCLEHGYCG 29

[Close]

BLAST of CmaCh16G003210 vs. Swiss-Prot
Match: ITR7_CYCPE (Trypsin inhibitor 7 OS=Cyclanthera pedata PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 7.5e-10
Identity = 24/29 (82.76%), Postives = 28/29 (96.55%), Query Frame = 1


Query: 59 RVCPRILMECKKDSDCLAECVCLEHGYCG 88
          R+CPRILM+CKKDSDCLAEC+C EHG+CG
Sbjct: 2  RICPRILMKCKKDSDCLAECICQEHGFCG 30

[Close]

BLAST of CmaCh16G003210 vs. Swiss-Prot
Match: ITR6_CYCPE (Trypsin inhibitor 6 OS=Cyclanthera pedata PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 9.8e-10
Identity = 24/29 (82.76%), Postives = 28/29 (96.55%), Query Frame = 1


Query: 59 RVCPRILMECKKDSDCLAECVCLEHGYCG 88
          R+CPRILM+CKKDSDCLAEC+C EHG+CG
Sbjct: 2  RICPRILMKCKKDSDCLAECICEEHGFCG 30

[Close]

BLAST of CmaCh16G003210 vs. TrEMBL
Match: A0A0A0KV23_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000835 PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.4e-15
Identity = 54/98 (55.10%), Postives = 67/98 (68.37%), Query Frame = 1


Query: 1  MGSNKIVTVALMALMLVA---SSSAAYAIDD-DSMDLVFDRR-----DINHGVPRKIM-K 60
          M S KIV VA++A ++V    SSSA++ +DD D + LV D R     D+N+G PRK+M K
Sbjct: 1  MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMIK 60

Query: 61 WGVLNHEERVCPRILMECKKDSDCLAECVCLEH-GYCG 88
           GV   EE +CPRILM+CK DSDCL  CVCLEH  YCG
Sbjct: 61 GGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEHIEYCG 98

[Close]

BLAST of CmaCh16G003210 vs. TrEMBL
Match: J3R9Z5_MOMCO (Two inhibitor peptide topologies 3 OS=Momordica cochinchinensis GN=TIPTOP3 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 4.9e-08
Identity = 39/90 (43.33%), Postives = 52/90 (57.78%), Query Frame = 1


Query: 1  MGSNKIVTVALMALMLVASSSAAYAIDDDSMDLVFDRR---DINHGVPRKIMKWGVLNHE 60
          M S KI+ V L+A+MLVA+S+     D D++DL+ D R   DIN GV             
Sbjct: 1  MESKKILPVVLVAMMLVATSTGFN--DGDTIDLISDGRAQIDINGGV------------- 60

Query: 61 ERVCPRILMECKKDSDCLAECVCLEHGYCG 88
             CP+IL  C++DSDC   C+CL +GYCG
Sbjct: 61 ---CPKILQRCRRDSDCPGACICLGNGYCG 72

[Close]

BLAST of CmaCh16G003210 vs. TrEMBL
Match: A0A0A7HIT2_9ROSI (TIPRE4 (Fragment) OS=Momordica anigosantha GN=TIPRE4 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.9e-07
Identity = 38/82 (46.34%), Postives = 49/82 (59.76%), Query Frame = 1


Query: 11 LMALMLVASSSAAYAIDDDSMDLVFDRR-----DINHGVPRKIMKWGVLNHEERVCPRIL 70
          L+A MLVA+S  A   D D++DL+ + R     DIN G        GV + E+R CPRIL
Sbjct: 4  LVATMLVATS--ADFNDGDTIDLISNDRAQTGQDINGG--------GVYSEEQRACPRIL 63

Query: 71 MECKKDSDCLAECVCLEHGYCG 88
            C++DSDC   CVC  +GYCG
Sbjct: 64 KRCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of CmaCh16G003210 vs. TrEMBL
Match: A0A0A7HF94_9ROSI (TIPRE3 (Fragment) OS=Momordica anigosantha GN=TIPRE3 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.9e-07
Identity = 38/82 (46.34%), Postives = 49/82 (59.76%), Query Frame = 1


Query: 11 LMALMLVASSSAAYAIDDDSMDLVFDRR-----DINHGVPRKIMKWGVLNHEERVCPRIL 70
          L+A MLVA+S  A   D D++DL+ + R     DIN G        GV + E+R CPRIL
Sbjct: 4  LVATMLVATS--ADFNDGDTIDLISNDRAQTGQDINGG--------GVYSEEQRACPRIL 63

Query: 71 MECKKDSDCLAECVCLEHGYCG 88
            C++DSDC   CVC  +GYCG
Sbjct: 64 KRCRRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of CmaCh16G003210 vs. TrEMBL
Match: J7IN40_9ROSI (Two inhibitor peptide topologies 2 OS=Momordica sphaeroidea GN=TIPTOP2 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.2e-07
Identity = 37/90 (41.11%), Postives = 51/90 (56.67%), Query Frame = 1


Query: 1  MGSNKIVTVALMALMLVASSSAAYAIDDDSMDLVFDRR---DINHGVPRKIMKWGVLNHE 60
          M S KI+ V L+A+MLVA+S+     D D++DL+ D R   DIN G+             
Sbjct: 1  MESKKILPVVLVAMMLVATSTGFN--DGDTIDLISDGRAQIDINGGI------------- 60

Query: 61 ERVCPRILMECKKDSDCLAECVCLEHGYCG 88
             CP+IL  C++DSDC   C+C  +GYCG
Sbjct: 61 ---CPKILQRCRRDSDCPGACICRGNGYCG 72

[Close]

BLAST of CmaCh16G003210 vs. NCBI nr
Match: gi|778697390|ref|XP_011654312.1| (PREDICTED: trypsin inhibitor 1-like [Cucumis sativus])

HSP 1 Score: 91.3 bits (225), Expect = 9.1e-16
Identity = 54/98 (55.10%), Postives = 69/98 (70.41%), Query Frame = 1


Query: 1  MGSNKIVTVALMALMLVA---SSSAAYAIDD-DSMDLVFDRR-----DINHGVPRKIM-K 60
          M S KIV VA++A +++    SSSA++ +DD DS+ LV D R     D+N+G PRK+M K
Sbjct: 1  MESKKIVVVAIVATIIMQAAMSSSASFVVDDKDSIKLVTDGRAQADQDMNNGCPRKMMIK 60

Query: 61 WGVLNHEERVCPRILMECKKDSDCLAECVCLEH-GYCG 88
           GV   EE VCP+ILM+CK DSDCL +CVCLE  GYCG
Sbjct: 61 GGVFKEEEMVCPKILMKCKHDSDCLLDCVCLEDIGYCG 98

[Close]

BLAST of CmaCh16G003210 vs. NCBI nr
Match: gi|700197606|gb|KGN52764.1| (hypothetical protein Csa_4G000835 [Cucumis sativus])

HSP 1 Score: 88.2 bits (217), Expect = 7.7e-15
Identity = 54/98 (55.10%), Postives = 67/98 (68.37%), Query Frame = 1


Query: 1  MGSNKIVTVALMALMLVA---SSSAAYAIDD-DSMDLVFDRR-----DINHGVPRKIM-K 60
          M S KIV VA++A ++V    SSSA++ +DD D + LV D R     D+N+G PRK+M K
Sbjct: 1  MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMIK 60

Query: 61 WGVLNHEERVCPRILMECKKDSDCLAECVCLEH-GYCG 88
           GV   EE +CPRILM+CK DSDCL  CVCLEH  YCG
Sbjct: 61 GGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEHIEYCG 98

[Close]

BLAST of CmaCh16G003210 vs. NCBI nr
Match: gi|125014|sp|P10293.1|ITR3_CUCPE (RecName: Full=Trypsin inhibitor 3; AltName: Full=CPTI-III; AltName: Full=Trypsin inhibitor III; Contains: RecName: Full=Trypsin inhibitor 2; AltName: Full=CPTI-II; AltName: Full=Trypsin inhibitor II)

HSP 1 Score: 75.9 bits (185), Expect = 4.0e-11
Identity = 30/32 (93.75%), Postives = 32/32 (100.00%), Query Frame = 1


Query: 56 HEERVCPRILMECKKDSDCLAECVCLEHGYCG 88
          HEERVCP+ILMECKKDSDCLAEC+CLEHGYCG
Sbjct: 1  HEERVCPKILMECKKDSDCLAECICLEHGYCG 32

[Close]

BLAST of CmaCh16G003210 vs. NCBI nr
Match: gi|125015|sp|P07853.2|ITR4_CUCMA (RecName: Full=Trypsin inhibitor 4; AltName: Full=CMTI-IV; AltName: Full=Trypsin inhibitor IV; Contains: RecName: Full=Trypsin inhibitor 3; AltName: Full=CMTI-III; AltName: Full=ITD-III; AltName: Full=Trypsin inhibitor III)

HSP 1 Score: 75.9 bits (185), Expect = 4.0e-11
Identity = 31/32 (96.88%), Postives = 32/32 (100.00%), Query Frame = 1


Query: 56 HEERVCPRILMECKKDSDCLAECVCLEHGYCG 88
          HEERVCPRILM+CKKDSDCLAECVCLEHGYCG
Sbjct: 1  HEERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of CmaCh16G003210 vs. NCBI nr
Match: gi|608796218|gb|AHW57457.1| (GB1-CMTI-III [synthetic construct])

HSP 1 Score: 70.9 bits (172), Expect = 1.3e-09
Identity = 29/36 (80.56%), Postives = 32/36 (88.89%), Query Frame = 1


Query: 52  GVLNHEERVCPRILMECKKDSDCLAECVCLEHGYCG 88
           G++    RVCPRILM+CKKDSDCLAECVCLEHGYCG
Sbjct: 72  GLVPRGSRVCPRILMKCKKDSDCLAECVCLEHGYCG 107

The following BLAST results are available for this feature:

BLAST of CmaCh16G003210 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ITR3_CUCPE	2.5e-13	93.75	Trypsin inhibitor 3 OS=Cucurbita pepo PE=1 SV=1	[more]
ITR4_CUCMA	2.5e-13	96.88	Trypsin inhibitor 4 OS=Cucurbita maxima PE=1 SV=2	[more]
ITR1_CUCMA	1.0e-11	100.00	Trypsin inhibitor 1 OS=Cucurbita maxima PE=1 SV=1	[more]
ITR7_CYCPE	7.5e-10	82.76	Trypsin inhibitor 7 OS=Cyclanthera pedata PE=1 SV=1	[more]
ITR6_CYCPE	9.8e-10	82.76	Trypsin inhibitor 6 OS=Cyclanthera pedata PE=1 SV=1	[more]

BLAST of CmaCh16G003210 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KV23_CUCSA	5.4e-15	55.10	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000835 PE=3 SV=1	[more]
J3R9Z5_MOMCO	4.9e-08	43.33	Two inhibitor peptide topologies 3 OS=Momordica cochinchinensis GN=TIPTOP3 PE=2 ...	[more]
A0A0A7HIT2_9ROSI	1.9e-07	46.34	TIPRE4 (Fragment) OS=Momordica anigosantha GN=TIPRE4 PE=3 SV=1	[more]
A0A0A7HF94_9ROSI	1.9e-07	46.34	TIPRE3 (Fragment) OS=Momordica anigosantha GN=TIPRE3 PE=3 SV=1	[more]
J7IN40_9ROSI	3.2e-07	41.11	Two inhibitor peptide topologies 2 OS=Momordica sphaeroidea GN=TIPTOP2 PE=3 SV=1	[more]

BLAST of CmaCh16G003210 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmaCh16G003210 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778697390\|ref\|XP_011654312.1\|	9.1e-16	55.10	PREDICTED: trypsin inhibitor 1-like [Cucumis sativus]	[more]
gi\|700197606\|gb\|KGN52764.1\|	7.7e-15	55.10	hypothetical protein Csa_4G000835 [Cucumis sativus]	[more]
gi\|125014\|sp\|P10293.1\|ITR3_CUCPE	4.0e-11	93.75	RecName: Full=Trypsin inhibitor 3; AltName: Full=CPTI-III; AltName: Full=Trypsin...	[more]
gi\|125015\|sp\|P07853.2\|ITR4_CUCMA	4.0e-11	96.88	RecName: Full=Trypsin inhibitor 4; AltName: Full=CMTI-IV; AltName: Full=Trypsin ...	[more]
gi\|608796218\|gb\|AHW57457.1\|	1.3e-09	80.56	GB1-CMTI-III [synthetic construct]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000737	Prot_inh_squash
IPR011052	Proteinase_amylase_inhib_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G003210.1	CmaCh16G003210.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PRINTS	PR00293	SQUASHINHBTR	coord: 71..80 score: 6.4E-6coord: 61..70 score: 6.
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 59..87 score: 2.1
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 61..80 scor
IPR011052	Proteinase/amylase inhibitor domain	unknown	SSF57027	Plant inhibitors of proteinases and amylases	coord: 56..87 score: 1.73
None	No IPR available	SMART	SM00286	PTI_1	coord: 59..87 score: 3.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh16G003210	Cla006424	Watermelon (97103) v1	cmawmB293
CmaCh16G003210	ClCG10G010420	Watermelon (Charleston Gray)	cmawcgB280
CmaCh16G003210	CSPI04G00370	Wild cucumber (PI 183967)	cmacpiB338
CmaCh16G003210	CmoCh16G003400	Cucurbita moschata (Rifu)	cmacmoB322
CmaCh16G003210	Lsi03G010230	Bottle gourd (USVL1VR-Ls)	cmalsiB307
CmaCh16G003210	Cla97C10G193330	Watermelon (97103) v2	cmawmbB332
CmaCh16G003210	Bhi11G000948	Wax gourd	cmawgoB0410
CmaCh16G003210	CsGy4G000330	Cucumber (Gy14) v2	cgybcmaB494

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh16G003210	Silver-seed gourd	carcmaB0406
CmaCh16G003210	Silver-seed gourd	carcmaB0526
CmaCh16G003210	Cucumber (Chinese Long) v3	cmacucB0388
CmaCh16G003210	Cucumber (Chinese Long) v3	cmacucB0413
CmaCh16G003210	Cucumber (Chinese Long) v3	cmacucB0414
CmaCh16G003210	Watermelon (97103) v2	cmawmbB342
CmaCh16G003210	Wax gourd	cmawgoB0446
CmaCh16G003210	Cucurbita maxima (Rimu)	cmacmaB135
CmaCh16G003210	Cucurbita maxima (Rimu)	cmacmaB340
CmaCh16G003210	Cucurbita maxima (Rimu)	cmacmaB351
CmaCh16G003210	Cucumber (Gy14) v1	cgycmaB0226
CmaCh16G003210	Cucumber (Gy14) v1	cgycmaB0197
CmaCh16G003210	Cucumber (Gy14) v1	cgycmaB0623
CmaCh16G003210	Cucurbita moschata (Rifu)	cmacmoB318
CmaCh16G003210	Cucurbita moschata (Rifu)	cmacmoB338
CmaCh16G003210	Wild cucumber (PI 183967)	cmacpiB351
CmaCh16G003210	Cucumber (Chinese Long) v2	cmacuB333
CmaCh16G003210	Cucumber (Chinese Long) v2	cmacuB347
CmaCh16G003210	Cucumber (Chinese Long) v2	cmacuB348
CmaCh16G003210	Melon (DHL92) v3.5.1	cmameB287
CmaCh16G003210	Melon (DHL92) v3.5.1	cmameB303
CmaCh16G003210	Melon (DHL92) v3.5.1	cmameB311
CmaCh16G003210	Watermelon (Charleston Gray)	cmawcgB293
CmaCh16G003210	Cucurbita pepo (Zucchini)	cmacpeB344
CmaCh16G003210	Cucurbita pepo (Zucchini)	cmacpeB351
CmaCh16G003210	Bottle gourd (USVL1VR-Ls)	cmalsiB304
CmaCh16G003210	Cucumber (Gy14) v2	cgybcmaB750
CmaCh16G003210	Melon (DHL92) v3.6.1	cmamedB331
CmaCh16G003210	Melon (DHL92) v3.6.1	cmamedB354
CmaCh16G003210	Melon (DHL92) v3.6.1	cmamedB363