CmaCh16G002170 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh16G002170
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Phosphofructokinase 4
Location	Cma_Chr16 : 985973 .. 990958 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGATCTGCTTGTTCTTTAAGAAGAGCGGTTAGTCATTAATCTCATGTCAAGACATATATTAAAGGAAATCGAAAATTTTAAGTTCCTTGTTCAATTTGTTTTTTTGGTTGTCTTGACGTGAGCAAATCAACCATGTTCAAATTGAGATGTCCTGCGATTTGAAAAGAGGAGTTATATATAATTTTAGTTTCCTTCCATAAAAATGGATTTTGATGTATTCCATTGGCATTGTCTAATTATCATTATGGGTAACACGTATTTGAAAGTGAACGCACTTTTTTAGTTCTAGTTGGCCCGACAATTAAAATTTTGAATGCATTTGAGTTTGATAATACTAAAATATTTATGGGCCATGGTTATATGAATATTTATTATCATAGTGATTCCTCATACTACTATCATGCATAAACCTGCAAAACCACTGATTTAAATTCATTACTCTGCTTTTCTTTAGTCGTATCCAAGTCCACTGAAAAACTGTCAGGCTTATGCAATTGTCAAGTAAGTGCAGCGATAATGTTTCAATTAGTTCTATTATTACAGTTTAATATGCGTCCTAATCAAGTTTTCTTATTTTATTTTGGAACCCCTTTTTGGTTATTACTTCTTAGGCTTGAAGTATTTCAGGGAAACTTTTATCAAGCCAGAAGATGTTGCACAAAAAATCGGTGTACTATCTCTCTCTCTTTTGCAATTTATCATTCTATTTTCTTTTTATTTAAACAACTTTTGTTGGTATAATTACCAATGCGAAATCTCTGTTCATCATGCACTGGTTCCAGATTGTAATTACGAAAGATAGCACTAGCGGAGTACATTTTTGGCATGCAGGTCCTCGTGAAAAGGTATCCCTTATTCTGATCATGTCTATTGAAACTAAAAGGATGAACTGAGAACTTTGTGATGCAGGTTTATTTCAAGTCAGATGAGGTGCGTGCTTTTGCAGTAACATGTGGGGGATTGTGTCCTGGGATTAACACAGTGGTTCGGTAGATTGTTTGTGGTCCACTATGGCTTTAACTATATGTATGGTGTGATGTACTTGGAATTGAGGTATGAAAAATATTTTTATTTTCCTAACTTTTTAAAATATTTTTTTATCCTATTATTATCTTTCAATTTAGTCTGTTATTTCTCATGGATGGTATAAAGATTAGCAGATTAATTGTTTTCGTTGTAGTTGTAATCTTTTTCCAGTTCTTGTAAATGTGTACATTTGAAATTCATGAAATTATCACAAGTTTCAATTCGTCTTTTCTTCCAAAGACCCTCTAATGTAGAGGGTCTACTTCCACTTACATTGAAAGCACACCCTTGCCAGGCATCACCACTGGATGTGCATTTTAGAATGACGAAAAGATGTCATTCATTTAATGAAATAAGCTGTATGCGCACAAGGAGAGATTATGATAGGGTGCTGAGGTTCAACAAACAAGTTCTTTGTGATCCAAAGATCGCTAAACTTGAGTAGTCCATTGCATAGAGACAAGTAGATGAAGTCACTCTTTGTAAATTTGATCCGGAATTTATTATCCTCACAGAGACCAAGCAGTTCATCTCTTAGAAGGTGGTTAAATATCTATGGAGCTCTGTAGATATTGTTTGGCCTGCTCTGGATGCATCTAACTTTTTCTGTATATCATCATTTTGTGGAAGGATAATTCTATTTTCGTTTCCGAAGTTGTGGAGGGGTCTTTCTCCATTTTGGTGGCTTTTACCTCGCCTGATGGTTTTGTTGAATTTATGACCACTATGACTTGGGCTCACTCTCTTTCTCCGATTTTTTTTCTATTTATCCAGTTAACCTCTTGGGATTCTGACCACAGATAAAGATTACTTCCCAATCTAAACTTAATCAAGTTGAATCAAAATCACAAGGTTCAAATAAATTTGTATTTGTTCATGAAATAGACACAATGTGATTTAGGACGAACATATAGTTCTGTTTTCAGCTCGTGACACTGTTAATCAGTTGGTTCACCGACTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACACATTCTCTTATGCTGTTGTTTGTCATCAGGGAGGATACAGAAGGTTTTACTCCAAGAATACATTGAAATTGACACCTAAAGTTGTCAACGATATCCTCTAACATGTTGGGTACTTTTCTTCGTACTTCACTAGGAGGCCATGATACAAATAAGACTGTTGATAATTTTCAAGATCGTGGAATGAATCAGGTACAAGTAAAAAGCTAGTTGTCTATTTCAGAGAAGAAGCGAGAGCAACAAAAAAATAAAATAATAACAATAAAAGAAGGAAAAAAATAAAATAAAATAAGAAGGAAAAATAAGGGAATGCTACAGAAAAATTAATGTCTTCCTATGGTCTAGGCGTATATCATTGGCGGCGATGGTACTCAAAGAAGAGCAGCTTTAATTTATCAGGTATTAACTCTTATTAGAATTTCACACTTGTTCCTTAACAATTCATTTGTTGGACTAGTAGTGTTGCCTTTCTTCTTCTAAAGGGAGTTGCAAAACGAGGTCTTCAAGTTTCTGTAGCTGGGATCCCCAAGACGATTGCTAATGCAATTGCTGTAAGTATTTTTCTGGACTTACAGAAAGAAACCTCATCTGTTGTCAATATTTTCATTATTTAGTCAAATATTCATTTGTAAATGATTAATTAGTTGCACATGAGGAAAGGCAAGATCATCTTACTTTTAGAACCTAACCATGGATGTCTATCCATTTGTACTTTCTGGTTTTAATGAAATTTATTCACGTTATGCACTGCATAGTGCGTACTGACCTAACTTGATTGATTATTTTCTCCCGACTGTATTGACAATTGGATGTATTGTTTTTGAATGACTCAATACTCTTTAGTGATGGAAGATACATTCGTATGAACACTTTTCATATGGATCTTTATGCACGGTGCATATTTCTTGATAATAAGACCAGAACTTGGATAAATTCATAGTTTGATCGGAAGTTTCTAGTGATTTGAGTGCTTAGATATCTACACATCTGAAGATCTTATCTTTAGTTCTATCGACGGAATTATAACTAGGACAGTGTGGATATCGTACATCGGCTCATGAAGGAAATGTTGGATGATTGTAGGGAAGTAGGACATTGTTAGGAATAGTAGTAAGGAAACATTAGGATATTAGGTCATATTAGTCACTATTTTTAACGGTGTTTCTCTGCAAGATAATTGTTTAAATTGGGTTGCTTTTATTGTTAGTTTTTTCCTTTTTTTTTTTCTCTCTCTCTCTCTGAGAGTTTATTTTTTAGCATTAATCTCTTTTTATTTTTTCATTGAAAAGTTTCATCACTTATTCAAAGGGTAGTCATAAATGGAGTGAATAGGTTGAAGGGTTTTCCTAAGGTAGACTAGAAATGGTTTTTGGGAGAGAACTTAGCCTCTCAATACCTTCGTAACTTTCTTGGTTAGTTCAATAAATTTTTGCTCTTGCCTCCTACTTTTCTAGATTTTCTTTTAATCTCTTTGTTGAGGAAACATATTTTATGAATGTGATCTATATCTTAGAGATATGGTAGGATAGTCTATTTGCATGTAAAATAATTAAGCTGATTTGTTTTGCTTGATGTTTGGCCTCTACCTGCCTACAAGAAACCATCATTTTGGTTAAGAGAAAAAAACAGAGAGAATTGTCATCAAAATTGACATTTGAAAATTCAAGCCAGTTGAACAGTTCAATTGGTAAAGATCAGCAAGAACCGATCATAAAATGAATGAAATTGATTTTTTCTTTCTTTCTTGAAAATGATTGAAATTGAACTGACTAAAATACTGTTAAGTAAATTTTTAACCTTCCCCTTCAATGGACTATAAAATGTTCTCGTAATTGTGAGGGTATTCAAATATCGAGACCTCAAATGTTTAAAATTTCACCCTATAATGTTAGAGCTAAAAAATCATTTATGTAAGGTAAGAACTATTTAGAGCCATCTGCAAGACGGCATTTTCTTCCATTTCCTTGTTGCAATGAGATTTTACCTCATTGAAAGTGGCATCACAAGAAAATGACACAACACAACCAACCCTCGTAAAACTATTTTTTCTTTTCTCAGAGTCAATCTCATATTGTTAGTTGTATATCTTCTTCTCCTCAACTTGTTCTTTGGTTTTATCCTTATCCATTCATGCACCTTCCTTAGGTTCTATAGGGATCACTCCTTCAATATGGACGATTCCCTCTCTCTATATAAGTCGCTTTCATTCTTTTTTTGAGGGACAGGGCAGCTGTGGGAGTGAAGGAAATGGACAATTCTGGGCCAATGGAAGTGACTCGACTTGACCTACATGTAAGGACAGGCGCAGCCTCTTTTTTGTTTGTTTCTTCATTAAATGATTGCTGGTTAACACACAGACAGCTTAACTAGTTTTTATCACATTATTCATAATGCACATATTTTGCTTCAAAGTTATCATATCACTTGACAGGATTTAGATGTATCTCTGTTCACATGGATGGGTATACAAAAATACTAAAATAGTAAAAAAAAAAAAAAAAAAAAAAAGTATGTTACTCTGGTAANAATTTTTCCCAAGCTTATTAGAGGTGACATTATTAGCTCATGACTGCCCATTCTCTTTTAAGAAATCTACTGTTGTTTGATTTAGACCATTTAGATTGTGTGGTGTTGTTGGGGAATGGTTGAAGAAAATTGTTTCTCCATACTTCTTTGTTTTTGGATGGTGATTCTTCTGAAACTGAGATGTTTTTACAGCAATGTTTTATTAGGTGGAGAGAGAATGTTCAAGTGCTCCTGGGGAAGCAATGAAAATCACTTCAAAGGTTAGTAAAAGTAGAGATTTCTTTTAGTGAAGTCTACATATATAATCATCGGTCTTTTTAAGTTGAGAGTATATTATATTAGTATAAAGATAGAGGTTAAAAGGTAGAATTGCATGAACAAGTGATAGTGGAG

mRNA sequence

ATGATGATCTGCTTGTTCTTTAAGAAGAGCGTCGTATCCAAGTCCACTGAAAAACTGTCAGGCTTATGCAATTGTCAAATTGTAATTACGAAAGATAGCACTAGCGGAGTACATTTTTGGCATGCAGGTCCTCGTGAAAAGGTTTATTTCAAGTCAGATGAGGTGCGTGCTTTTGCAGTAACATGTGGGGGATTGTGTCCTGGGATTAACACAGTGGTTCGGGAGGATACAGAAGGAGGCCATGATACAAATAAGACTGTTGATAATTTTCAAGATCGTGGAATGAATCAGGCGTATATCATTGGCGGCGATGGTACTCAAAGAAGAGCAGCTTTAATTTATCAGGGAGTTGCAAAACGAGGTCTTCAAGTTTCTGTAGCTGGGATCCCCAAGACGATTGCTAATGCAATTGCTGGCAGCTGTGGGAGTGAAGGAAATGGACAATTCTGGGCCAATGGAAGTGACTCGACTTGACCTACATCAATGTTTTATTAGGTGGAGAGAGAATGTTCAAGTGCTCCTGGGGAAGCAATGAAAATCACTTCAAAGGTTAGTAAAAGTAGAGATTTCTTTTAGTGAAGTCTACATATATAATCATCGGTCTTTTTAAGTTGAGAGTATATTATATTAGTATAAAGATAGAGGTTAAAAGGTAGAATTGCATGAACAAGTGATAGTGGAG

Coding sequence (CDS)

ATGATGATCTGCTTGTTCTTTAAGAAGAGCGTCGTATCCAAGTCCACTGAAAAACTGTCAGGCTTATGCAATTGTCAAATTGTAATTACGAAAGATAGCACTAGCGGAGTACATTTTTGGCATGCAGGTCCTCGTGAAAAGGTTTATTTCAAGTCAGATGAGGTGCGTGCTTTTGCAGTAACATGTGGGGGATTGTGTCCTGGGATTAACACAGTGGTTCGGGAGGATACAGAAGGAGGCCATGATACAAATAAGACTGTTGATAATTTTCAAGATCGTGGAATGAATCAGGCGTATATCATTGGCGGCGATGGTACTCAAAGAAGAGCAGCTTTAATTTATCAGGGAGTTGCAAAACGAGGTCTTCAAGTTTCTGTAGCTGGGATCCCCAAGACGATTGCTAATGCAATTGCTGGCAGCTGTGGGAGTGAAGGAAATGGACAATTCTGGGCCAATGGAAGTGACTCGACTTGA

Protein sequence

MMICLFFKKSVVSKSTEKLSGLCNCQIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVREDTEGGHDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQGVAKRGLQVSVAGIPKTIANAIAGSCGSEGNGQFWANGSDST

Homology

[Close]

BLAST of CmaCh16G002170 vs. Swiss-Prot
Match: PFKA3_ARATH (ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana GN=PFK3 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 9.0e-30
Identity = 72/161 (44.72%), Postives = 91/161 (56.52%), Query Frame = 1


Query: 26  QIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE---------- 85
           +IV+ KD   G+HF  AGPR+KVYF+SDEV A  VTCGGLCPG+NTV+RE          
Sbjct: 63  KIVVHKDGPRGIHFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYG 122

Query: 86  -----DTEGGH----------------------------------DTNKTVDNFQDRGMN 138
                  +GG+                                  DT K VD+ QDRG+N
Sbjct: 123 VKRILGIDGGYRGFYAKNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGIN 182

[Close]

BLAST of CmaCh16G002170 vs. Swiss-Prot
Match: PFKA6_ARATH (ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana GN=PFK6 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.9e-18
Identity = 55/141 (39.01%), Postives = 78/141 (55.32%), Query Frame = 1


Query: 5   LFFKKSVVSKSTEKLSGLCNCQIVITKDSTSGVHFWHAGPREKV--------YFKSDEVR 64
           ++FK S V        GLC     + ++   G+HF + G  E +        ++  + V 
Sbjct: 86  VYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMY-GVTEVIGVDCGFRGFYSKNTVA 145

Query: 65  AFAVTCGGLCPGINTVVREDTEGGHDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQG 124
               T   +     T++   + GGHDT+K VDN QDR +NQ YIIGGDGTQ+ A  IY+ 
Sbjct: 146 LTPKTVSDIHKRGGTILGT-SRGGHDTSKIVDNIQDREINQVYIIGGDGTQKGANAIYKE 205

Query: 125 VAKRGLQVSVAGIPKTIANAI 138
           + +RGL+V+VAGIPKTI N I
Sbjct: 206 IRRRGLKVAVAGIPKTIDNDI 224

[Close]

BLAST of CmaCh16G002170 vs. Swiss-Prot
Match: PFKA4_ARATH (ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana GN=PFK4 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.3e-16
Identity = 42/64 (65.62%), Postives = 50/64 (78.12%), Query Frame = 1


Query: 75  EDTEGGHDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQGVAKRGLQVSVAGIPKTIA 134
           + + GGHDT K VDN QDRG+NQ YIIGG GTQ+ A  IY+ V +RGLQV+V+GIPKTI 
Sbjct: 212 QTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEKIYEEVERRGLQVAVSGIPKTID 271

Query: 135 NAIA 139
           N IA
Sbjct: 272 NDIA 275

[Close]

BLAST of CmaCh16G002170 vs. Swiss-Prot
Match: PFKA7_ARATH (ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana GN=PFK7 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-15
Identity = 39/61 (63.93%), Postives = 50/61 (81.97%), Query Frame = 1


Query: 77  TEGGHDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQGVAKRGLQVSVAGIPKTIANA 136
           + GGHDTNK VD+ QDRG+NQ YIIGGDGTQR A++I++ + +R L+V+V GIPKTI N 
Sbjct: 163 SRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGASVIFEEIRRRRLKVAVVGIPKTIDND 222

Query: 137 I 138
           I
Sbjct: 223 I 223

[Close]

BLAST of CmaCh16G002170 vs. Swiss-Prot
Match: PFKA1_ARATH (ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana GN=PFK1 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.8e-14
Identity = 38/61 (62.30%), Postives = 49/61 (80.33%), Query Frame = 1


Query: 77  TEGGHDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQGVAKRGLQVSVAGIPKTIANA 136
           + GGH+T K VD+ QDRG+NQ YIIGGDG+Q+ AA I++ + KR L+V+VAGIPKTI N 
Sbjct: 164 SRGGHNTTKIVDSIQDRGINQVYIIGGDGSQKGAAAIFEEIRKRKLKVAVAGIPKTIDND 223

Query: 137 I 138
           I
Sbjct: 224 I 224

[Close]

BLAST of CmaCh16G002170 vs. TrEMBL
Match: A0A097QH96_9ROSA (ATP-dependent 6-phosphofructokinase OS=Prunus sibirica GN=PFK PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.2e-30
Identity = 74/161 (45.96%), Postives = 93/161 (57.76%), Query Frame = 1


Query: 26  QIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE---------- 85
           +IV+ KDS  G HF  +GPR+KVYF+SDEV A  VTCGGLCPG+NTV+RE          
Sbjct: 119 KIVVHKDSPRGTHFRRSGPRQKVYFESDEVHACIVTCGGLCPGLNTVIRELVCGLTHMYH 178

Query: 86  ---------------------------------------DTEGGHDTNKTVDNFQDRGMN 138
                                                   + GGHDT+K VD+ QDRG+N
Sbjct: 179 VKRILGIEGGYRGFYARNTVPITPKVVNDIHKRGGTILGTSRGGHDTSKIVDSIQDRGIN 238

[Close]

BLAST of CmaCh16G002170 vs. TrEMBL
Match: K4CLI8_SOLLC (ATP-dependent 6-phosphofructokinase OS=Solanum lycopersicum GN=PFK PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.7e-30
Identity = 75/161 (46.58%), Postives = 91/161 (56.52%), Query Frame = 1


Query: 26  QIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE---------- 85
           +IV+ KDS  GVHF  AGPR+KVYF SD+VRA  VTCGGLCPG+NTV+RE          
Sbjct: 63  KIVVHKDSQRGVHFRRAGPRQKVYFNSDDVRACIVTCGGLCPGLNTVIREIVHSLDYMYG 122

Query: 86  ---------------------------------------DTEGGHDTNKTVDNFQDRGMN 138
                                                   + GGHDT K VD+ QD G+N
Sbjct: 123 VDKVLGIEGGYRGFYAKNTINLTPKLVNDIHKRGGTILGTSRGGHDTKKIVDSIQDHGIN 182

[Close]

BLAST of CmaCh16G002170 vs. TrEMBL
Match: A0A166FRS9_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_000769 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 6.3e-30
Identity = 74/161 (45.96%), Postives = 91/161 (56.52%), Query Frame = 1


Query: 26  QIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE---------- 85
           ++V+ KDS  G HF  AGPR+KVYF SDEV A  VTCGGLCPG+NTV+RE          
Sbjct: 70  KVVVHKDSPRGTHFRRAGPRQKVYFSSDEVHACIVTCGGLCPGLNTVIRELVCALYHMYG 129

Query: 86  ---------------------------------------DTEGGHDTNKTVDNFQDRGMN 138
                                                   + GGHDT K VD+ QDRG+N
Sbjct: 130 VTRVLGIEGGYRGFYSRNTISLTPKIVNDIHKRGGTILGTSRGGHDTMKIVDSIQDRGIN 189

[Close]

BLAST of CmaCh16G002170 vs. TrEMBL
Match: U5D4D3_AMBTC (ATP-dependent 6-phosphofructokinase OS=Amborella trichopoda GN=PFK PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 8.2e-30
Identity = 77/177 (43.50%), Postives = 97/177 (54.80%), Query Frame = 1


Query: 10  SVVSKSTEKLSGLCNCQIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGI 69
           SVV++    +      +IV+ K+S  G HF  AGPREKVYF+ DEV A  VTCGGLCPG+
Sbjct: 44  SVVTQYFVNVDDTVTQKIVVHKNSPRGTHFRRAGPREKVYFEPDEVSACIVTCGGLCPGV 103

Query: 70  NTVVRE-------------------------------------------------DTEGG 129
           NTV+RE                                                  + GG
Sbjct: 104 NTVIREIVCGLYHMYGVTNVLGIEAGYKGFYSRNTIPLTPKFVNDIHKRGGTIIGTSRGG 163

Query: 130 HDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQGVAKRGLQVSVAGIPKTIANAI 138
           HDT+K VD+ QDRG+NQ YIIGGDGTQ+ AA I++ + KRG++V+VAGIPKTI N I
Sbjct: 164 HDTSKIVDSIQDRGINQVYIIGGDGTQKGAACIFEEILKRGIKVAVAGIPKTIDNDI 220

[Close]

BLAST of CmaCh16G002170 vs. TrEMBL
Match: A0A0D6R203_ARACU (ATP-dependent 6-phosphofructokinase OS=Araucaria cunninghamii GN=PFK PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 8.2e-30
Identity = 74/161 (45.96%), Postives = 93/161 (57.76%), Query Frame = 1


Query: 27  IVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE----------- 86
           IV+  +S  G+HF  AGPRE+VYFKSDEVRA  VTCGGLCPGINTV+RE           
Sbjct: 134 IVVRLNSPRGLHFRRAGPREEVYFKSDEVRACIVTCGGLCPGINTVIREIVCGLWNMYGV 193

Query: 87  --------------------------------------DTEGGHDTNKTVDNFQDRGMNQ 139
                                                  + GGH T++ VD+ QDRG+NQ
Sbjct: 194 QNVIGIEGGYKGFYSCNTIPLNSKVVNDIHKRGGTVLGTSRGGHVTSRIVDSIQDRGINQ 253

[Close]

BLAST of CmaCh16G002170 vs. TAIR10
Match: AT4G26270.1 (AT4G26270.1 phosphofructokinase 3)

HSP 1 Score: 131.3 bits (329), Expect = 5.1e-31
Identity = 72/161 (44.72%), Postives = 91/161 (56.52%), Query Frame = 1


Query: 26  QIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE---------- 85
           +IV+ KD   G+HF  AGPR+KVYF+SDEV A  VTCGGLCPG+NTV+RE          
Sbjct: 63  KIVVHKDGPRGIHFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYG 122

Query: 86  -----DTEGGH----------------------------------DTNKTVDNFQDRGMN 138
                  +GG+                                  DT K VD+ QDRG+N
Sbjct: 123 VKRILGIDGGYRGFYAKNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGIN 182

[Close]

BLAST of CmaCh16G002170 vs. TAIR10
Match: AT4G32840.1 (AT4G32840.1 phosphofructokinase 6)

HSP 1 Score: 91.7 bits (226), Expect = 4.4e-19
Identity = 55/141 (39.01%), Postives = 78/141 (55.32%), Query Frame = 1


Query: 5   LFFKKSVVSKSTEKLSGLCNCQIVITKDSTSGVHFWHAGPREKV--------YFKSDEVR 64
           ++FK S V        GLC     + ++   G+HF + G  E +        ++  + V 
Sbjct: 86  VYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMY-GVTEVIGVDCGFRGFYSKNTVA 145

Query: 65  AFAVTCGGLCPGINTVVREDTEGGHDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQG 124
               T   +     T++   + GGHDT+K VDN QDR +NQ YIIGGDGTQ+ A  IY+ 
Sbjct: 146 LTPKTVSDIHKRGGTILGT-SRGGHDTSKIVDNIQDREINQVYIIGGDGTQKGANAIYKE 205

Query: 125 VAKRGLQVSVAGIPKTIANAI 138
           + +RGL+V+VAGIPKTI N I
Sbjct: 206 IRRRGLKVAVAGIPKTIDNDI 224

[Close]

BLAST of CmaCh16G002170 vs. TAIR10
Match: AT5G61580.1 (AT5G61580.1 phosphofructokinase 4)

HSP 1 Score: 85.9 bits (211), Expect = 2.4e-17
Identity = 42/64 (65.62%), Postives = 50/64 (78.12%), Query Frame = 1


Query: 75  EDTEGGHDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQGVAKRGLQVSVAGIPKTIA 134
           + + GGHDT K VDN QDRG+NQ YIIGG GTQ+ A  IY+ V +RGLQV+V+GIPKTI 
Sbjct: 212 QTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEKIYEEVERRGLQVAVSGIPKTID 271

Query: 135 NAIA 139
           N IA
Sbjct: 272 NDIA 275

[Close]

BLAST of CmaCh16G002170 vs. TAIR10
Match: AT5G56630.1 (AT5G56630.1 phosphofructokinase 7)

HSP 1 Score: 84.3 bits (207), Expect = 7.1e-17
Identity = 39/61 (63.93%), Postives = 50/61 (81.97%), Query Frame = 1


Query: 77  TEGGHDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQGVAKRGLQVSVAGIPKTIANA 136
           + GGHDTNK VD+ QDRG+NQ YIIGGDGTQR A++I++ + +R L+V+V GIPKTI N 
Sbjct: 163 SRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGASVIFEEIRRRRLKVAVVGIPKTIDND 222

Query: 137 I 138
           I
Sbjct: 223 I 223

[Close]

BLAST of CmaCh16G002170 vs. TAIR10
Match: AT4G29220.1 (AT4G29220.1 phosphofructokinase 1)

HSP 1 Score: 80.5 bits (197), Expect = 1.0e-15
Identity = 38/61 (62.30%), Postives = 49/61 (80.33%), Query Frame = 1


Query: 77  TEGGHDTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQGVAKRGLQVSVAGIPKTIANA 136
           + GGH+T K VD+ QDRG+NQ YIIGGDG+Q+ AA I++ + KR L+V+VAGIPKTI N 
Sbjct: 164 SRGGHNTTKIVDSIQDRGINQVYIIGGDGSQKGAAAIFEEIRKRKLKVAVAGIPKTIDND 223

Query: 137 I 138
           I
Sbjct: 224 I 224

[Close]

BLAST of CmaCh16G002170 vs. NCBI nr
Match: gi|659107814|ref|XP_008453872.1| (PREDICTED: 6-phosphofructokinase 4, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 161.0 bits (406), Expect = 1.7e-36
Identity = 94/178 (52.81%), Postives = 105/178 (58.99%), Query Frame = 1


Query: 10  SVVSKSTEKLSGLCNCQIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGI 69
           ++V ++  K   +   +IV+TKDST GVHF  AGPREKVYFK DEVRA  VTCGGLCPGI
Sbjct: 101 AIVKETFVKPEDVVAQKIVVTKDSTRGVHFRRAGPREKVYFKPDEVRACVVTCGGLCPGI 160

Query: 70  NTVVRE---------------DTEGGH--------------------------------- 129
           NTVVRE                 EGG+                                 
Sbjct: 161 NTVVREIVCGLNYMYGVNDILGIEGGYRGFYSKNTLTLTPKVVNNIHKHGGTYLRTSRGG 220

Query: 130 -DTNKTVDNFQDRGMNQAYIIGGDGTQRRAALIYQGVAKRGLQVSVAGIPKTIANAIA 139
            DTNK VDN QDRG+NQ YIIGGDGTQR AALIYQ +AKRGLQV+VAGIPKTI N IA
Sbjct: 221 HDTNKIVDNIQDRGINQVYIIGGDGTQRGAALIYQEIAKRGLQVAVAGIPKTIDNDIA 278

[Close]

BLAST of CmaCh16G002170 vs. NCBI nr
Match: gi|659107818|ref|XP_008453875.1| (PREDICTED: 6-phosphofructokinase 4, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 160.6 bits (405), Expect = 2.2e-36
Identity = 92/162 (56.79%), Postives = 98/162 (60.49%), Query Frame = 1


Query: 26  QIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE---------- 85
           +IV+TKDST GVHF  AGPREKVYFK DEVRA  VTCGGLCPGINTVVRE          
Sbjct: 23  KIVVTKDSTRGVHFRRAGPREKVYFKPDEVRACVVTCGGLCPGINTVVREIVCGLNYMYG 82

Query: 86  -----DTEGGH----------------------------------DTNKTVDNFQDRGMN 139
                  EGG+                                  DTNK VDN QDRG+N
Sbjct: 83  VNDILGIEGGYRGFYSKNTLTLTPKVVNNIHKHGGTYLRTSRGGHDTNKIVDNIQDRGIN 142

[Close]

BLAST of CmaCh16G002170 vs. NCBI nr
Match: gi|1009121864|ref|XP_015877692.1| (PREDICTED: ATP-dependent 6-phosphofructokinase 4, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 153.7 bits (387), Expect = 2.7e-34
Identity = 86/161 (53.42%), Postives = 93/161 (57.76%), Query Frame = 1


Query: 27  IVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE----------- 86
           IV+ KDS  GVHF  AGPREKVYFKSDEVRA  V CGGLCPGINTV+RE           
Sbjct: 138 IVVQKDSPRGVHFRRAGPREKVYFKSDEVRACIVACGGLCPGINTVIRELVCALNYMYGV 197

Query: 87  --------------------------------------DTEGGHDTNKTVDNFQDRGMNQ 139
                                                  + GGHDT K VDN QDRG+NQ
Sbjct: 198 DDILGIEGGYKGFYSKNTLTLTTKVVNDIHKRGGTFLKTSRGGHDTKKIVDNIQDRGINQ 257

[Close]

BLAST of CmaCh16G002170 vs. NCBI nr
Match: gi|1012173876|ref|XP_015966428.1| (PREDICTED: ATP-dependent 6-phosphofructokinase 3-like [Arachis duranensis])

HSP 1 Score: 140.6 bits (353), Expect = 2.4e-30
Identity = 78/161 (48.45%), Postives = 93/161 (57.76%), Query Frame = 1


Query: 26  QIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE---------- 85
           ++V+ KDS  GVHF  AGPR+KVYF+SDEV+A  VTCGGLCPG+NTV+RE          
Sbjct: 114 KVVVHKDSERGVHFRRAGPRQKVYFESDEVKAAIVTCGGLCPGLNTVIRELVCGLHHMYG 173

Query: 86  -----DTEG----------------------------------GHDTNKTVDNFQDRGMN 138
                  EG                                  GHDTNK VD+ QDRG+N
Sbjct: 174 VKSVLGIEGGYRGFYARNTINLTPKCVNDIHKRGGTVLGTSRGGHDTNKIVDSIQDRGIN 233

[Close]

BLAST of CmaCh16G002170 vs. NCBI nr
Match: gi|700284249|gb|AIU64848.1| (6-phosphofructokinase [Prunus sibirica])

HSP 1 Score: 140.2 bits (352), Expect = 3.1e-30
Identity = 74/161 (45.96%), Postives = 93/161 (57.76%), Query Frame = 1


Query: 26  QIVITKDSTSGVHFWHAGPREKVYFKSDEVRAFAVTCGGLCPGINTVVRE---------- 85
           +IV+ KDS  G HF  +GPR+KVYF+SDEV A  VTCGGLCPG+NTV+RE          
Sbjct: 119 KIVVHKDSPRGTHFRRSGPRQKVYFESDEVHACIVTCGGLCPGLNTVIRELVCGLTHMYH 178

Query: 86  ---------------------------------------DTEGGHDTNKTVDNFQDRGMN 138
                                                   + GGHDT+K VD+ QDRG+N
Sbjct: 179 VKRILGIEGGYRGFYARNTVPITPKVVNDIHKRGGTILGTSRGGHDTSKIVDSIQDRGIN 238

The following BLAST results are available for this feature:

BLAST of CmaCh16G002170 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PFKA3_ARATH	9.0e-30	44.72	ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana GN=PFK3 PE=1 SV=1	[more]
PFKA6_ARATH	7.9e-18	39.01	ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana GN=PFK6 PE=1 SV=1	[more]
PFKA4_ARATH	4.3e-16	65.63	ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana GN=...	[more]
PFKA7_ARATH	1.3e-15	63.93	ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana GN=PFK7 PE=1 SV=1	[more]
PFKA1_ARATH	1.8e-14	62.30	ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana GN=PFK1 PE=1 SV=1	[more]

BLAST of CmaCh16G002170 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A097QH96_9ROSA	2.2e-30	45.96	ATP-dependent 6-phosphofructokinase OS=Prunus sibirica GN=PFK PE=2 SV=1	[more]
K4CLI8_SOLLC	3.7e-30	46.58	ATP-dependent 6-phosphofructokinase OS=Solanum lycopersicum GN=PFK PE=3 SV=1	[more]
A0A166FRS9_DAUCA	6.3e-30	45.96	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_000769 PE=4 SV=1	[more]
U5D4D3_AMBTC	8.2e-30	43.50	ATP-dependent 6-phosphofructokinase OS=Amborella trichopoda GN=PFK PE=3 SV=1	[more]
A0A0D6R203_ARACU	8.2e-30	45.96	ATP-dependent 6-phosphofructokinase OS=Araucaria cunninghamii GN=PFK PE=3 SV=1	[more]

BLAST of CmaCh16G002170 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G26270.1	5.1e-31	44.72	phosphofructokinase 3	[more]
AT4G32840.1	4.4e-19	39.01	phosphofructokinase 6	[more]
AT5G61580.1	2.4e-17	65.63	phosphofructokinase 4	[more]
AT5G56630.1	7.1e-17	63.93	phosphofructokinase 7	[more]
AT4G29220.1	1.0e-15	62.30	phosphofructokinase 1	[more]

BLAST of CmaCh16G002170 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659107814\|ref\|XP_008453872.1\|	1.7e-36	52.81	PREDICTED: 6-phosphofructokinase 4, chloroplastic isoform X1 [Cucumis melo]	[more]
gi\|659107818\|ref\|XP_008453875.1\|	2.2e-36	56.79	PREDICTED: 6-phosphofructokinase 4, chloroplastic isoform X2 [Cucumis melo]	[more]
gi\|1009121864\|ref\|XP_015877692.1\|	2.7e-34	53.42	PREDICTED: ATP-dependent 6-phosphofructokinase 4, chloroplastic [Ziziphus jujuba...	[more]
gi\|1012173876\|ref\|XP_015966428.1\|	2.4e-30	48.45	PREDICTED: ATP-dependent 6-phosphofructokinase 3-like [Arachis duranensis]	[more]
gi\|700284249\|gb\|AIU64848.1\|	3.1e-30	45.96	6-phosphofructokinase [Prunus sibirica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000023	Phosphofructokinase_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0003872	6-phosphofructokinase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006096	glycolytic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006000	fructose metabolic process
biological_process	GO:0006012	galactose metabolic process
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0006096	glycolytic process
biological_process	GO:0006013	mannose metabolic process
biological_process	GO:0006098	pentose-phosphate shunt
biological_process	GO:0006002	fructose 6-phosphate metabolic process
biological_process	GO:0061615	glycolytic process through fructose-6-phosphate
cellular_component	GO:0005945	6-phosphofructokinase complex
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003872	6-phosphofructokinase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G002170.1	CmaCh16G002170.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000023	Phosphofructokinase domain	PFAM	PF00365	PFK	coord: 82..140 score: 2.
IPR000023	Phosphofructokinase domain	unknown	SSF53784	Phosphofructokinase	coord: 34..138 score: 2.75
None	No IPR available	GENE3D	G3DSA:3.40.50.450		coord: 76..138 score: 2.2E-13coord: 30..75 score: 1.
None	No IPR available	PANTHER	PTHR13697	PHOSPHOFRUCTOKINASE	coord: 27..138 score: 7.3
None	No IPR available	PANTHER	PTHR13697:SF33	6-PHOSPHOFRUCTOKINASE 4, CHLOROPLASTIC	coord: 27..138 score: 7.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh16G002170	CmoCh04G003780	Cucurbita moschata (Rifu)	cmacmoB338
CmaCh16G002170	Cp4.1LG01g03500	Cucurbita pepo (Zucchini)	cmacpeB351
CmaCh16G002170	MELO3C017646.2	Melon (DHL92) v3.6.1	cmamedB355
CmaCh16G002170	CsaV3_4G004200	Cucumber (Chinese Long) v3	cmacucB0387
CmaCh16G002170	Cla97C07G144010	Watermelon (97103) v2	cmawmbB352
CmaCh16G002170	Bhi01G002007	Wax gourd	cmawgoB0440
CmaCh16G002170	Bhi11G000814	Wax gourd	cmawgoB0410
CmaCh16G002170	CsGy4G004050	Cucumber (Gy14) v2	cgybcmaB493
CmaCh16G002170	Carg08034	Silver-seed gourd	carcmaB0526

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh16G002170	Cucumber (Gy14) v1	cgycmaB0196
CmaCh16G002170	Cucurbita moschata (Rifu)	cmacmoB322
CmaCh16G002170	Wild cucumber (PI 183967)	cmacpiB337
CmaCh16G002170	Cucumber (Chinese Long) v2	cmacuB332
CmaCh16G002170	Melon (DHL92) v3.5.1	cmameB304
CmaCh16G002170	Watermelon (Charleston Gray)	cmawcgB302
CmaCh16G002170	Watermelon (97103) v1	cmawmB313
CmaCh16G002170	Cucurbita pepo (Zucchini)	cmacpeB342
CmaCh16G002170	Cucurbita pepo (Zucchini)	cmacpeB344
CmaCh16G002170	Bottle gourd (USVL1VR-Ls)	cmalsiB301
CmaCh16G002170	Bottle gourd (USVL1VR-Ls)	cmalsiB323
CmaCh16G002170	Silver-seed gourd	carcmaB0995
CmaCh16G002170	Silver-seed gourd	carcmaB1206
CmaCh16G002170	Cucumber (Chinese Long) v3	cmacucB0405
CmaCh16G002170	Watermelon (97103) v2	cmawmbB329
CmaCh16G002170	Cucurbita maxima (Rimu)	cmacmaB351