CmaCh16G001860 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh16G001860
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Plant invertase/pectin methylesterase inhibitor
Location	Cma_Chr16 : 818987 .. 819571 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCTTTCAATTTGCAAGAAAAGTTTGATTTTTGTGCAAATAATTCTCCAATTAACACTCATTCATTCAGCCATCACTCCACAAAGCAGCACACAGTTCATAAGAACTTCTTGCACTTCCACTAAATACCCTCGACTCTGTTTCTCATCTCTTTCAATCCACGCCAATTCCATCCAAACAAGCCCTCGCCTTTTGGCCACCACCGCCCTCTCCGTCACCCTCTCCTCCGTCAAGTCCGCCGCCACCCAAATCCTCAAGCTCTCCCACGCCCACGGCCTCCCTACAAGAGACGTCTCTGCTCTTAACGACTGTTTGGAAGAGCTCAGTGACTCTGTTGACTCCCTTGCCAGCTCCATTTCCGAGATGCCCAAGCTACGTGGCCATGACTTTGATCTCGTTATGAGCGATGTTCAGACATGGGTCAGCGCCGCCCTCACCGATGAGACCACTTGTAGTGAGGGGTTTCAAGGGAAGGCTGTCAAGGGCGGCGTTAAGGCGGCGGTTAGGAGGAGGATTGTGAACATTGCGCAATTGACCAGCAATGCTCTGTCTTTGATCAACCGGATTGCAGAGCTGCATTAG

mRNA sequence

ATGGGTCTTTCAATTTGCAAGAAAAGTTTGATTTTTGTGCAAATAATTCTCCAATTAACACTCATTCATTCAGCCATCACTCCACAAAGCAGCACACAGTTCATAAGAACTTCTTGCACTTCCACTAAATACCCTCGACTCTGTTTCTCATCTCTTTCAATCCACGCCAATTCCATCCAAACAAGCCCTCGCCTTTTGGCCACCACCGCCCTCTCCGTCACCCTCTCCTCCGTCAAGTCCGCCGCCACCCAAATCCTCAAGCTCTCCCACGCCCACGGCCTCCCTACAAGAGACGTCTCTGCTCTTAACGACTGTTTGGAAGAGCTCAGTGACTCTGTTGACTCCCTTGCCAGCTCCATTTCCGAGATGCCCAAGCTACGTGGCCATGACTTTGATCTCGTTATGAGCGATGTTCAGACATGGGTCAGCGCCGCCCTCACCGATGAGACCACTTGTAGTGAGGGGTTTCAAGGGAAGGCTGTCAAGGGCGGCGTTAAGGCGGCGGTTAGGAGGAGGATTGTGAACATTGCGCAATTGACCAGCAATGCTCTGTCTTTGATCAACCGGATTGCAGAGCTGCATTAG

Coding sequence (CDS)

ATGGGTCTTTCAATTTGCAAGAAAAGTTTGATTTTTGTGCAAATAATTCTCCAATTAACACTCATTCATTCAGCCATCACTCCACAAAGCAGCACACAGTTCATAAGAACTTCTTGCACTTCCACTAAATACCCTCGACTCTGTTTCTCATCTCTTTCAATCCACGCCAATTCCATCCAAACAAGCCCTCGCCTTTTGGCCACCACCGCCCTCTCCGTCACCCTCTCCTCCGTCAAGTCCGCCGCCACCCAAATCCTCAAGCTCTCCCACGCCCACGGCCTCCCTACAAGAGACGTCTCTGCTCTTAACGACTGTTTGGAAGAGCTCAGTGACTCTGTTGACTCCCTTGCCAGCTCCATTTCCGAGATGCCCAAGCTACGTGGCCATGACTTTGATCTCGTTATGAGCGATGTTCAGACATGGGTCAGCGCCGCCCTCACCGATGAGACCACTTGTAGTGAGGGGTTTCAAGGGAAGGCTGTCAAGGGCGGCGTTAAGGCGGCGGTTAGGAGGAGGATTGTGAACATTGCGCAATTGACCAGCAATGCTCTGTCTTTGATCAACCGGATTGCAGAGCTGCATTAG

Protein sequence

MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAELH

Homology

[Close]

BLAST of CmaCh16G001860 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.2e-37
Identity = 87/195 (44.62%), Postives = 132/195 (67.69%), Query Frame = 1


Query: 3   LSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTS 62
           + + K +L+F  +++ L    +A +   + QFI+TSCT T YP +C  SLS +A +IQ +
Sbjct: 1   MKLSKSTLVFSALLVIL----AAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNN 60

Query: 63  PRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISE 122
           P+ LA+TAL V+L+  + A T + +L+   GL  R  +A++DCLEE+ DS+D ++ S  E
Sbjct: 61  PQELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDE 120

Query: 123 MPKL---RGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQL 182
           M  L   +G+DF   MS+V+TWVSAALTDETTC +GF GK + G +K +VR ++V +A++
Sbjct: 121 MKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARV 180

Query: 183 TSNALSLINRIAELH 195
           TSNAL+L+N  A  H
Sbjct: 181 TSNALALVNNFAAKH 191

[Close]

BLAST of CmaCh16G001860 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 6.5e-14
Identity = 57/176 (32.39%), Postives = 100/176 (56.82%), Query Frame = 1


Query: 26  ITPQSSTQ--FIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAAT 85
           IT  SST    +++ C+ST YP LCFS+++       TS + +   +L++T  +VK    
Sbjct: 51  ITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYF 110

Query: 86  QILKL-SHAHGLPTRDVSALNDCLEELSDSVDSLASSISEM---PK---LRGHDFDLVMS 145
            + KL +   GL  R+V+AL+DCLE + +++D L  ++ ++   PK   LR H       
Sbjct: 111 AVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKH-----AD 170

Query: 146 DVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           D++T +S+A+T++ TC +GF        V+ A+ +  V++  + SNAL++I  + E
Sbjct: 171 DLKTLISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTE 221

[Close]

BLAST of CmaCh16G001860 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-12
Identity = 48/170 (28.24%), Postives = 95/170 (55.88%), Query Frame = 1


Query: 30  SSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKL- 89
           SS   +R+SC+ST+YP LC S++        TS + +   ++++T+++V+     + KL 
Sbjct: 54  SSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLI 113

Query: 90  SHAHGLPTRDVSALNDCLEELSDSVDSLASSISEM------PKLRGHDFDLVMSDVQTWV 149
               GL  R+ +AL+DCLE + +++D L  ++ ++        LR H       D++T +
Sbjct: 114 KKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREH-----AGDLKTLI 173

Query: 150 SAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           S+A+T++ TC +GF        V+ A+ +  +++  + SNAL++I  + +
Sbjct: 174 SSAITNQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTD 218

[Close]

BLAST of CmaCh16G001860 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.1e-12
Identity = 47/161 (29.19%), Postives = 92/161 (57.14%), Query Frame = 1


Query: 35  IRTSCTSTKYPRLCFSSLSI--HANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHAH 94
           +++SC+ST+YP LCFS+++    A+   TS + +   +L++T ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 95  GLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGH-DFDLVMSDVQTWVSAALTDETT 154
            L  R+  AL+DCLE + +++D L  ++ ++ +            D++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 155 CSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           C +GF        V+ A+    V++ ++ SNAL++I  + +
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of CmaCh16G001860 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.1e-12
Identity = 47/161 (29.19%), Postives = 92/161 (57.14%), Query Frame = 1


Query: 35  IRTSCTSTKYPRLCFSSLSI--HANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHAH 94
           +++SC+ST+YP LCFS+++    A+   TS + +   +L++T ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 95  GLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGH-DFDLVMSDVQTWVSAALTDETT 154
            L  R+  AL+DCLE + +++D L  ++ ++ +            D++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 155 CSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           C +GF        V+ A+    V++ ++ SNAL++I  + +
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of CmaCh16G001860 vs. TrEMBL
Match: A0A0A0LB27_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 1.6e-80
Identity = 163/194 (84.02%), Postives = 181/194 (93.30%), Query Frame = 1


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MG+S  KK+LI  Q+ILQLTLIHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP+RDVSAL+DCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRG +FDL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIA+LH
Sbjct: 181 SNALSLINRIADLH 194

[Close]

BLAST of CmaCh16G001860 vs. TrEMBL
Match: A0A0A0L8D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 6.2e-80
Identity = 162/193 (83.94%), Postives = 179/193 (92.75%), Query Frame = 1


Query: 2   GLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQT 61
           G+S  KKSLI  Q+ILQLTLIHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSIS 121
           SPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP+RDVSAL+DCLEELSDSVDSLA+SIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTS 181
           EMPKLRG +FDL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINRIAELH 195
           NALSLINRI +LH
Sbjct: 224 NALSLINRIGDLH 236

[Close]

BLAST of CmaCh16G001860 vs. TrEMBL
Match: M5XKR0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.6e-51
Identity = 109/179 (60.89%), Postives = 140/179 (78.21%), Query Frame = 1


Query: 16  ILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTL 75
           I+  T     + P+++T+FI+TSC++T YP+LC +SLS HA++IQTSP+L+A  AL+VTL
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDFDLVM 135
           SS KS +  +LKLS +HGL  ++V A+ DCLEELSDSVD L SSI+EM   + +DF L++
Sbjct: 84  SSAKSTSAVMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           SDVQTWVSAALTDE TCSEGF G  + G +K AVR RIVNIAQLTSNAL+LINR A +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]

BLAST of CmaCh16G001860 vs. TrEMBL
Match: B9ICT6_POPTR (Ripening-related family protein OS=Populus trichocarpa GN=POPTR_0015s14690g PE=4 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 5.7e-49
Identity = 108/190 (56.84%), Postives = 146/190 (76.84%), Query Frame = 1


Query: 5   ICKKSLIFVQIILQLTLIHSA-ITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSP 64
           +  KSLI + I   +    +A +TPQSS +FIRTSC++T YPRLC++SLSIH+ +I TSP
Sbjct: 4   LTSKSLILIAISFFINSSSAATVTPQSSIEFIRTSCSTTTYPRLCYTSLSIHSRTIHTSP 63

Query: 65  RLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEM 124
           +L+A  AL+VTLSS KS +T + KLS +HGL  ++VSA+ DC+EELSD+V  L  SI EM
Sbjct: 64  KLIANAALNVTLSSAKSTSTMMSKLSQSHGLKPKEVSAMKDCVEELSDAVYELRESIDEM 123

Query: 125 PKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNA 184
             ++  DF++++SDV+TWVSAA+TDE+TCS+GF G A+ G +K AVR RI+NIAQLTSNA
Sbjct: 124 DHVKRSDFEVMISDVRTWVSAAMTDESTCSDGFAGNAMNGNLKRAVRGRIMNIAQLTSNA 183

Query: 185 LSLINRIAEL 194
           L+L+N  A L
Sbjct: 184 LALVNNYALL 193

[Close]

BLAST of CmaCh16G001860 vs. TrEMBL
Match: A0A061G793_THECC (DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 2.2e-48
Identity = 108/197 (54.82%), Postives = 143/197 (72.59%), Query Frame = 1


Query: 4   SICKKSLIFVQIILQLTLIHS------AITPQSSTQFIRTSCTSTKYPRLCFSSLSIHAN 63
           + C  S  F+ I+L +    +       I PQSS +FIRTSC+ST YP+LCF +LS HA+
Sbjct: 4   TFCSHSFKFLLILLAINSYINFSSAGRQIAPQSSAEFIRTSCSSTTYPKLCFETLSTHAS 63

Query: 64  SIQTSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLA 123
            IQTSP+LLA  AL+VTLS+ +S +  ++ LS +HGL  R+  A+ DC+EELSDS+D L 
Sbjct: 64  LIQTSPQLLAHAALNVTLSTTESTSAMMVTLSKSHGLKPREAEAMQDCVEELSDSIDELR 123

Query: 124 SSISEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIA 183
            SISEM +++G +F L+++DVQTWVSAALTDE+TCS+GFQG  + G VK AVR +I+ IA
Sbjct: 124 KSISEMGQVKGSNFGLMINDVQTWVSAALTDESTCSDGFQGNNMNGNVKTAVRTKILRIA 183

Query: 184 QLTSNALSLINRIAELH 195
            LTSNAL+LIN  A LH
Sbjct: 184 HLTSNALALINNYASLH 200

[Close]

BLAST of CmaCh16G001860 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 174.9 bits (442), Expect = 4.9e-44
Identity = 94/187 (50.27%), Postives = 137/187 (73.26%), Query Frame = 1


Query: 12  FVQIILQLTLIHSAITPQSST----QFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLA 71
           F+ II  L L+H+  T  ++T    +F+++SCT T YPRLCFSSLS HA+ IQTSP+L+A
Sbjct: 17  FLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMA 76

Query: 72  TTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLR 131
             AL++TL+S K  +  +++LS++  L  ++VSA+ DC+EEL D+++ L  SI EM +L 
Sbjct: 77  HAALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLS 136

Query: 132 GHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLI 191
           G ++++ +SD+QTWVSAALTD  TC++GF+G+ + G VK  VR RI+ IA LTSNAL+LI
Sbjct: 137 GSNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALI 196

Query: 192 NRIAELH 195
           N  A +H
Sbjct: 197 NHFASIH 202

[Close]

BLAST of CmaCh16G001860 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 148.7 bits (374), Expect = 3.8e-36
Identity = 88/195 (45.13%), Postives = 123/195 (63.08%), Query Frame = 1


Query: 10  LIFVQIILQLTLIHSAITPQSSTQ-----FIRTSCTSTKYPRLCFSSLSIHANSIQTSPR 69
           L+ +  +L L L  +    Q+        FI++SC +T YP LC  SLS++AN IQTSP+
Sbjct: 9   LLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPK 68

Query: 70  LLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMP 129
            LA TA++VTLS  +S    + +L+   GL  R+V A+ DC+EE++D+VD L  S+ E+ 
Sbjct: 69  RLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQEL- 128

Query: 130 KLRG-----HDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQL 189
           KL G       F   MS+ QTW SAALTDE TCS+GF G+ + G +K +VR RI+N+   
Sbjct: 129 KLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHE 188

Query: 190 TSNALSLINRIAELH 195
           TSNALSLIN  A+ +
Sbjct: 189 TSNALSLINAFAKTY 202

[Close]

BLAST of CmaCh16G001860 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 146.7 bits (369), Expect = 1.4e-35
Identity = 79/167 (47.31%), Postives = 113/167 (67.66%), Query Frame = 1


Query: 32  TQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHA 91
           T+FI+ SC +T YP  CF SLS +A+ I+  PR LA TAL+V+++  KSA T + +++  
Sbjct: 35  TEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDY 94

Query: 92  HGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLR----GHDFDLVMSDVQTWVSAALT 151
            G+  R   A+ DCLEE+ D+VD L++S+ E+  L     G DF   +S+V+TW SAALT
Sbjct: 95  KGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALT 154

Query: 152 DETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           DET C +GF GKA+ G +K+ +R  IV++A+ TSNAL+LIN  A  H
Sbjct: 155 DETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]

BLAST of CmaCh16G001860 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 142.9 bits (359), Expect = 2.1e-34
Identity = 82/171 (47.95%), Postives = 118/171 (69.01%), Query Frame = 1


Query: 24  SAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAAT 83
           S  + Q++  +I+TSC  T Y  +C++SLS +A++I+++P+ LA  AL++TLSS KSA+ 
Sbjct: 137 STYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASK 196

Query: 84  QILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGHD---FDLVMSDVQT 143
            +  +SH  GL   +V A+ DC+EE+ DSV SL  SI E+  +   D   F++VMSDV+T
Sbjct: 197 FVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVET 256

Query: 144 WVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIA 192
           WVSAALT++ TC +GF    VK  VK  VRR +V +A+LTSNAL+LIN  A
Sbjct: 257 WVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of CmaCh16G001860 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 141.4 bits (355), Expect = 6.0e-34
Identity = 81/173 (46.82%), Postives = 115/173 (66.47%), Query Frame = 1


Query: 24  SAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAAT 83
           +AIT + +  FI+ SC +T YP +C +SL+ +ANSIQTSPR LA TAL+VT++  +S   
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 84  QILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMP---KLRGHD-FDLVMSDVQ 143
            + +L     L  R++ A+ DC+EE+ D+VD L  SI E+      +G D F   MS+ Q
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQ 147

Query: 144 TWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           TW SAALT+  TCS+GF G+ + G VK +VR RI+N+ + TSNAL+LIN  A+
Sbjct: 148 TWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAK 200

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: gi|778679325|ref|XP_004148138.2| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 307.0 bits (785), Expect = 2.4e-80
Identity = 163/194 (84.02%), Postives = 181/194 (93.30%), Query Frame = 1


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MG+S  KK+LI  Q+ILQLTLIHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP+RDVSAL+DCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRG +FDL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIA+LH
Sbjct: 181 SNALSLINRIADLH 194

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: gi|778688522|ref|XP_011652768.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 305.1 bits (780), Expect = 9.0e-80
Identity = 162/193 (83.94%), Postives = 179/193 (92.75%), Query Frame = 1


Query: 2   GLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQT 61
           G+S  KKSLI  Q+ILQLTLIHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSIS 121
           SPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP+RDVSAL+DCLEELSDSVDSLA+SIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTS 181
           EMPKLRG +FDL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINRIAELH 195
           NALSLINRI +LH
Sbjct: 224 NALSLINRIGDLH 236

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: gi|596006342|ref|XP_007218426.1| (hypothetical protein PRUPE_ppa011607mg [Prunus persica])

HSP 1 Score: 209.5 bits (532), Expect = 5.1e-51
Identity = 109/179 (60.89%), Postives = 140/179 (78.21%), Query Frame = 1


Query: 16  ILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTL 75
           I+  T     + P+++T+FI+TSC++T YP+LC +SLS HA++IQTSP+L+A  AL+VTL
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDFDLVM 135
           SS KS +  +LKLS +HGL  ++V A+ DCLEELSDSVD L SSI+EM   + +DF L++
Sbjct: 84  SSAKSTSAVMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           SDVQTWVSAALTDE TCSEGF G  + G +K AVR RIVNIAQLTSNAL+LINR A +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: gi|645257371|ref|XP_008234384.1| (PREDICTED: 21 kDa protein [Prunus mume])

HSP 1 Score: 208.0 bits (528), Expect = 1.5e-50
Identity = 109/179 (60.89%), Postives = 139/179 (77.65%), Query Frame = 1


Query: 16  ILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTL 75
           I+  T     + P+++T+FI+TSC +T YP+LC +SLS HA++IQTSP+L+A  AL+VTL
Sbjct: 24  IISSTSAARDLAPKTNTEFIKTSCRATTYPKLCRTSLSSHASTIQTSPKLMAGAALNVTL 83

Query: 76  SSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDFDLVM 135
           SS KS +  +LKLS +HGL  ++V A+ DCLEELSDSVD L SSI+EM   + +DF L++
Sbjct: 84  SSAKSTSATMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEMGNFKSYDFQLMI 143

Query: 136 SDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           SDVQTWVSAALTDE TCSEGF G  + G +K AVR RIVNIAQLTSNAL+LINR A +H
Sbjct: 144 SDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNALALINRYASVH 202

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: gi|566207616|ref|XP_002322404.2| (ripening-related family protein [Populus trichocarpa])

HSP 1 Score: 202.2 bits (513), Expect = 8.2e-49
Identity = 108/190 (56.84%), Postives = 146/190 (76.84%), Query Frame = 1


Query: 5   ICKKSLIFVQIILQLTLIHSA-ITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSP 64
           +  KSLI + I   +    +A +TPQSS +FIRTSC++T YPRLC++SLSIH+ +I TSP
Sbjct: 4   LTSKSLILIAISFFINSSSAATVTPQSSIEFIRTSCSTTTYPRLCYTSLSIHSRTIHTSP 63

Query: 65  RLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEM 124
           +L+A  AL+VTLSS KS +T + KLS +HGL  ++VSA+ DC+EELSD+V  L  SI EM
Sbjct: 64  KLIANAALNVTLSSAKSTSTMMSKLSQSHGLKPKEVSAMKDCVEELSDAVYELRESIDEM 123

Query: 125 PKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNA 184
             ++  DF++++SDV+TWVSAA+TDE+TCS+GF G A+ G +K AVR RI+NIAQLTSNA
Sbjct: 124 DHVKRSDFEVMISDVRTWVSAAMTDESTCSDGFAGNAMNGNLKRAVRGRIMNIAQLTSNA 183

Query: 185 LSLINRIAEL 194
           L+L+N  A L
Sbjct: 184 LALVNNYALL 193

The following BLAST results are available for this feature:

BLAST of CmaCh16G001860 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	3.2e-37	44.62	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]
PME2_ARATH	6.5e-14	32.39	Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2	[more]
PME3_ARATH	2.1e-12	28.24	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S...	[more]
PME3_CITSI	6.1e-12	29.19	Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1	[more]
PME1_CITSI	6.1e-12	29.19	Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1	[more]

BLAST of CmaCh16G001860 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB27_CUCSA	1.6e-80	84.02	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1	[more]
A0A0A0L8D1_CUCSA	6.2e-80	83.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1	[more]
M5XKR0_PRUPE	3.6e-51	60.89	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1	[more]
B9ICT6_POPTR	5.7e-49	56.84	Ripening-related family protein OS=Populus trichocarpa GN=POPTR_0015s14690g PE=4...	[more]
A0A061G793_THECC	2.2e-48	54.82	DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1	[more]

BLAST of CmaCh16G001860 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62360.1	4.9e-44	50.27	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	3.8e-36	45.13	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	1.4e-35	47.31	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	2.1e-34	47.95	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	6.0e-34	46.82	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of CmaCh16G001860 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778679325\|ref\|XP_004148138.2\|	2.4e-80	84.02	PREDICTED: 21 kDa protein [Cucumis sativus]	[more]
gi\|778688522\|ref\|XP_011652768.1\|	9.0e-80	83.94	PREDICTED: 21 kDa protein-like [Cucumis sativus]	[more]
gi\|596006342\|ref\|XP_007218426.1\|	5.1e-51	60.89	hypothetical protein PRUPE_ppa011607mg [Prunus persica]	[more]
gi\|645257371\|ref\|XP_008234384.1\|	1.5e-50	60.89	PREDICTED: 21 kDa protein [Prunus mume]	[more]
gi\|566207616\|ref\|XP_002322404.2\|	8.2e-49	56.84	ripening-related family protein [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G001860.1	CmaCh16G001860.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 31..190 score: 1.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..186 score: 3.5
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..186 score: 2.1
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 7..189 score: 1.6
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..190 score: 4.32
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 10..194 score: 2.1
None	No IPR available	PANTHER	PTHR31080:SF19	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 10..194 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh16G001860	MELO3C006819	Melon (DHL92) v3.5.1	cmameB296
CmaCh16G001860	CmoCh04G003490	Cucurbita moschata (Rifu)	cmacmoB338
CmaCh16G001860	Cp4.1LG13g02110	Cucurbita pepo (Zucchini)	cmacpeB342
CmaCh16G001860	MELO3C006819.2	Melon (DHL92) v3.6.1	cmamedB344

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh16G001860	Watermelon (97103) v2	cmawmbB346
CmaCh16G001860	Wax gourd	cmawgoB0442
CmaCh16G001860	Cucurbita maxima (Rimu)	cmacmaB345
CmaCh16G001860	Cucurbita maxima (Rimu)	cmacmaB351
CmaCh16G001860	Cucumber (Gy14) v1	cgycmaB0711
CmaCh16G001860	Cucurbita moschata (Rifu)	cmacmoB322
CmaCh16G001860	Wild cucumber (PI 183967)	cmacpiB335
CmaCh16G001860	Cucumber (Chinese Long) v2	cmacuB329
CmaCh16G001860	Watermelon (Charleston Gray)	cmawcgB296
CmaCh16G001860	Watermelon (97103) v1	cmawmB316
CmaCh16G001860	Watermelon (97103) v1	cmawmB322
CmaCh16G001860	Cucurbita pepo (Zucchini)	cmacpeB344
CmaCh16G001860	Cucurbita pepo (Zucchini)	cmacpeB351
CmaCh16G001860	Bottle gourd (USVL1VR-Ls)	cmalsiB312
CmaCh16G001860	Cucumber (Gy14) v2	cgybcmaB342
CmaCh16G001860	Silver-seed gourd	carcmaB0526
CmaCh16G001860	Silver-seed gourd	carcmaB1206
CmaCh16G001860	Silver-seed gourd	carcmaB1264
CmaCh16G001860	Cucumber (Chinese Long) v3	cmacucB0383