CmaCh15G007230 (gene) Cucurbita maxima (Rimu)

NameCmaCh15G007230
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptiontRNA modification GTPase MnmE
LocationCma_Chr15 : 3508254 .. 3513117 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCGAATCAGAGCTATGGCTCTGCTTCCAGGATTCCGCCATTTCATCACCCATTTTTGCAGAACCACGCCGCCAATGGCATTTCTCTTTACCCATTTCCATACCCCTATTTCCAGGCCATCCTCTCTCTTCCATATACCCAACCCCTCCAGCCCTGTTCTCCCGAAATCCCTGATAAAGTCCCACGCTTCTGGGAAAAGTCATTCCCCTAATCAATTTCTCTTATTTTCACTTCAACTTTACTGGGTTTGTCCATTTTCTCATTCTTGTTTCCTCTTAGTGCAGAAACTACATTCTTTTTGACTCGGGATGAGCGATTGGAGGATTCGCATGCCGGGATTGAGCGTGAACAAATTGAGAATTCAAGCACAATTGCAGCCATCGTGACATCCATGGGGGGTCCTCCTGCTGCGGTGGGCATTGTGCGTTTATCAGGTCCTCGTGCTGTGAATATCGTTGGGAGTTTATTTTGCCCTGCCGCGAAAAAGAAGGGGAAGAATGTGAGCCAGCAGCATCCATGGAGACCCACCAGCCATGTCGTGGAGTACGGTGTGGTATTGGACCAACAGGGCGATGTAATTGACGAAGTACAGTAATGTTTACTTTGCTATGCTTGGAAAATTTCTCAATTCTGCGTTATGCTGTTATGGGTGTAGGCTTTTGTTAGTTTATGATTAATTTGAGCTGTGGTTGTGCACTTTTGTGAAGGTTTTGACGGTGCCAATGTTAGCCCCAAGATCTTATACTCGTGAAGATGTGATCGAGATTCAGTGTCACGGAAGTGAAGTATGTCTTCGTCGTGTGTTAAAAGCTTGTTTAGAAGCTGGAGCCCGGCTTGCAGAACCAGGTTTGTTTCTGAACTGGAATCACTTAGTCATTAAATTCATTGTAAGATCCCACATCGGTTGGGGAGGAGAACAAAACACCCACCAGAACCGAGTGATCTTCCTGCGAGGAGGTTGAGCCCGAAATGGGGTGGACATGAGGCAATGTGCCAACAAGGACACTAGGCCCCAAAAGGGTAGATTGTGAGATCCCACATCCACATCGGTTGGGAAAGAGAAAGAAACACCCTTCTTAAGGGTCTGGAAACTCTCCCTAGCAGACACGTTTTAAAAACCTTGAAGGGAAGCCCAAAACGGAAATCTCAAAAAGAACAATATTTGCTAGCAGTGGGCTTGGGCGTTACATGGTTTGAAGGGTCTGGTAACTCTCCCTAGCAGACACGTTTTAAAAACCTTGAGGGGAAACTCAAAACGGAAAGCTCAAAGAGAACAATATTTGCTAACGGTGGGCTTGGGTGTTACATGGTCTATATACGTGCCCCTTTTCATTTTTATACATTCACTGAAGTGTTTGCTTGCATCCATGTAATTAAAGCATAGGCTAACGCATGAATTTATACTTTAGGTGAATTTACCCTTCGTGCATTCTTAAATGGTCGCTTAGACCTTTCACAAGCAGAAAATGTTGGGAAATTAATTTCAGCCAAGTCTACGGCTGCTGCTGATGCTGCGTTGGCTGGAATTCAGGTTGGGGTCTTCTTTATTTGACTGATAACGTTGCCTGAATGGCTGCCGACTTGTTTACTGAAGTATATTGTATTTTGGTAGGGTGGCTTCTCTTCCTTAGTCAAATTGCTAAGAACACAATGCATCGAATTACTCACTGAAATCGAGGCCCGACTAGACTTCGATGATGAGATGCCACCGTTGAATTTGGAAACTATAATGGAGAAAGTGCATGCCATGGCACAGGAAGTAGAGACTGCTCTCGAGACAGCCAATTATGACAAGCTTCTTCAATCTGGAATACAGGTAAACTTTTAAGAGTCGTCGAATCAGTTTGTTTCCTGGCACAAGAGATGCGTGCATTTGTTAATACCTTCTGTCATCTCCTTAAAACTTGCAGATAGCAATTGTTGGCCGTCCTAATGTTGGGAAGTCGAGCCTTCTTAATGCATGGAGCAAAGTAAGCTGCATAACGTCCTTGTTTTCCCTTTCAACTTGAACTCCTTTAGATGATTTATTCTATCTGTGAGTCTGATGTGATATCCGATAATCTTCATGTCAGTTCTTTCTTTTTCCTTGTTATTTCCGTTGAGTGGTTGTATTTTCACTTAATCTTGAAATCACCTACTGCTTTATCACATGGGAGAGCTTGCTTGACTTTCCAGATTTGGTGATATGTGACTGAGAAATCTTGGTCCTTGTTACTACTTTCATACCAGTAATGGTTTGTGAGATCCCATATCGGTTGGGGAGGAGAACGAAGCATTCCTTATAAAGGTGTGGAAACTTCTCCCTAGTAGACGCGTTTTAAAATCGTGAGGCTGACAACGATATGTAACGGACCAGAGCGGATGATATCTACTAGTAGTGGGCTTGGGCTATTACATTGCTGGAGAAAGAAATTGTAAACTTCTTAAAAATGCTAAAAGATTACAAACCTCATAATGGAAATTTCTGTTTGATATAGTGGTTTTCTTTTACTCTTAGTCACATTATTCCATTGTCACATTTATTATTTAGTGTGCAGAGCGAGAGGGCAATAGTTACAGAAATTGCTGGAACTACCAGGGATGTAATTGAAGCGAATGTTACCGTTTCTGGTATTCCTGTTACTCTTCTTGATACTGCTGGAATTAGGGAAACAGATGACATTGTGGAAAAGATAGGTATGATTTACTAATTTCAGCATTTCGAGGGTTTAGACAAAACATTTGGTGTTGTGCCATACAGATATATGATTATTTTCCCGAAACGAGTTGATTGCAGGCAGGTCACACAAACCATGTCGGTGTAGGTTGTAACTCGACCTACACCGACCATACATTCCCTTAAATCTTATTGACAACTCGACCATATGTTGAGTTGCCCTTAATTCTTCTCGAGTTGCAACCCGACAATACGTTCTCTACTCCTTGGTCACGGCCCTACTCTGTTCTACAGGTGTCGAGAGATCTGAAGCTGCTGCCCTCGGAGCTGATGTCATTATTATGACTATAAGTGCCCTCGATGGATGGACTGAAGAAGATACCATACTTCTCGATAGCATACTTGCAAAAAAGGTTTGACAGCAAAATCCTACCACCCTCTGCCTTCAATGAGTTCTGCTGATCAAAATTGGTGCTTATGTTTTCTTCTCTTTTAAATTCTTTGAAAGTACAGAAATCAGATGGATCAACCACGCCTATACTTCTTGTCATAAACAAGATAGACTGTGCTCCATCTGCTGCAAACATGGATGCAATTAGTATCAACCGTGACTCTTTCAGTAAACAAGTTTTTACATGCGCTGTCACTGGACAAGGAATAGAGCATCTGGAGATGGCAATATCAGAGCTGATGGGACTCAACAATACTCTTGCAAGTGGACGTAGATGGACGGTAAACCAGGTAACCTTTTCCTACCTTCCCCTCACCAAACAAGATTGAAAGAGTAATGAGCAATATAGAACTTATGGGTTAGTGTTGTTTTGGTCATACAATCAGAGACAATGCGAGCAGCTTCTTAGAACCAAGGAGGCACTTGGAAGATTGAAATCTTCGATTGAAGAGGAGCTGCCTCTTGACTTTTGGACAGTGGATTTGAGAGGTGCTGTATTAGCTCTTGGGGAAATTAGTGGTGAGGATATCTCTGAAGAGGTTTTGTCCAATATTTTTGGCAAGTTCTGTATTGGTAAGTAGAAAGTATCAATGTAATGTACTATTTGGTTATGAAATCCAATTATGTAAATGGATTTCATATTATTTTCCATTTCTAGCCATGGCTTCACTCGAGTCACTTTCAGGATGCCATCCCATTTTGGTCGCTTCCAGCTTCCAACATGTGGAAGTTTTTTCTGACCTGTGAACAGAGTGGTTGCAAATGTCTTTTAAACTCTCATAGGTCCTTGAACTTTTTTATTTTATCTCATAGCGTCTTAATTTATTCAACATATCATTTGACTTACAAATCGAGAGATAGTTTGACAATATAAGCCATCGTCATCTTCAACTGTGATTTTGTATCCAAGTGTCATCTGCATAGTTTGATGAAGGATGTAAAAGGGTGGTTTTCGTTTCGACCGTTGCTTGAGAAGAGTCTTTCAGATCAACTAACAGGCACAACTCTTCAAAGATGCAACACCATTGAAGATTTTATCCTCAACTCAAAATCACAAGGCCGTAGCACTCTGAAAAGGTGCCACACCATTGAAGGGTGTAGGGTTGTGTGTTTAAGCTGCTCCTCTCTCAGCTGTTGCCCTTGAAACGCAGTTTGTTCTCATGAGATTACACATGTTCGGAACGTTTTGAGGCTTAACTTTTCAGTTGCCTCGGATGGTCACTACAGGTAAAAGGATATGCTAGATTCTTTGTAAAGCTCAATAAGGAATAATAAATATTTTAGGTGCTGGCCGGTTCTTTCCCCCCTCCTTACCAGATGCTGCAAAAGCCATAAAAATATAGGTATATAGGACACCCTGCAGGCCATTGGACGGCAATGGCTATTTAAGTAGAATCATTTTGATGCTTGAGATTACATAACTCTGAAGGATAACCACAGTTTAAAATCATATCCTCCTGACAACCTCTAAAGGGGTTAAACTTGGTATAGAACCAATGTGTTTCAACATCAGAAGAAAGCTTACAAAAGAAGAAATGAAGGAGGAAGAGAAGAAAAGGAGAGCAAAGGAAAAGGAAAAACAGAGGAAGGAAGAAGAGAGGAAAGCCAAGGAGAAGAAGGAACGTGAAAAACAAAGGAAGAAAGAAGAGAGGAAAGCCGAGGAGACGAGGGAAAAGGAAGAAAATCTGAGAAACAAAAGGAAACAGCAGAAAGCATTGAAGATAGAGAAACAGAAAAACAAGACAAAGAGGAAATAA

mRNA sequence

TTCGAATCAGAGCTATGGCTCTGCTTCCAGGATTCCGCCATTTCATCACCCATTTTTGCAGAACCACGCCGCCAATGGCATTTCTCTTTACCCATTTCCATACCCCTATTTCCAGGCCATCCTCTCTCTTCCATATACCCAACCCCTCCAGCCCTGTTCTCCCGAAATCCCTGATAAAGTCCCACGCTTCTGGGAAAAAAACTACATTCTTTTTGACTCGGGATGAGCGATTGGAGGATTCGCATGCCGGGATTGAGCGTGAACAAATTGAGAATTCAAGCACAATTGCAGCCATCGTGACATCCATGGGGGGTCCTCCTGCTGCGGTGGGCATTGTGCGTTTATCAGGTCCTCGTGCTGTGAATATCGTTGGGAGTTTATTTTGCCCTGCCGCGAAAAAGAAGGGGAAGAATGTGAGCCAGCAGCATCCATGGAGACCCACCAGCCATGTCGTGGAGTACGGTGTGGTATTGGACCAACAGGGCGATGTTTTGACGGTGCCAATGTTAGCCCCAAGATCTTATACTCGTGAAGATGTGATCGAGATTCAGTGTCACGGAAGTGAAGTATGTCTTCGTCGTGAATTTACCCTTCGTGCATTCTTAAATGGTCGCTTAGACCTTTCACAAGCAGAAAATGTTGGGAAATTAATTTCAGCCAAGTCTACGGCTGCTGCTGATGCTGCGTTGGCTGGAATTCAGGGTGGCTTCTCTTCCTTAGTCAAATTGCTAAGAACACAATGCATCGAATTACTCACTGAAATCGAGGCCCGACTAGACTTCGATGATGAGATGCCACCGTTGAATTTGGAAACTATAATGGAGAAAGTGCATGCCATGGCACAGGAAGTAGAGACTGCTCTCGAGACAGCCAATTATGACAAGCTTCTTCAATCTGGAATACAGATAGCAATTGTTGGCCGTCCTAATGTTGGGAAGTCGAGCCTTCTTAATGCATGGAGCAAAAGCGAGAGGGCAATAGTTACAGAAATTGCTGGAACTACCAGGGATGTAATTGAAGCGAATGTTACCGTTTCTGGTATTCCTGTTACTCTTCTTGATACTGCTGGAATTAGGGAAACAGATGACATTGTGGAAAAGATAGGTGTCGAGAGATCTGAAGCTGCTGCCCTCGGAGCTGATGTCATTATTATGACTATAAGTGCCCTCGATGGATGGACTGAAGAAGATACCATACTTCTCGATAGCATACTTGCAAAAAAGAAATCAGATGGATCAACCACGCCTATACTTCTTGTCATAAACAAGATAGACTGTGCTCCATCTGCTGCAAACATGGATGCAATTAGTATCAACCGTGACTCTTTCAGTAAACAAGTTTTTACATGCGCTGTCACTGGACAAGGAATAGAGCATCTGGAGATGGCAATATCAGAGCTGATGGGACTCAACAATACTCTTGCAAGTGGACGTAGATGGACGGTAAACCAGAGACAATGCGAGCAGCTTCTTAGAACCAAGGAGGCACTTGGAAGATTGAAATCTTCGATTGAAGAGGAGCTGCCTCTTGACTTTTGGACAGTGGATTTGAGAGGTGCTGTATTAGCTCTTGGGGAAATTAGTGGTGAGGATATCTCTGAAGAGGTTTTGTCCAATATTTTTGGCAAGTTCTGTATTGAACCAATGTGTTTCAACATCAGAAGAAAGCTTACAAAAGAAGAAATGAAGGAGGAAGAGAAGAAAAGGAGAGCAAAGGAAAAGGAAAAACAGAGGAAGGAAGAAGAGAGGAAAGCCAAGGAGAAGAAGGAACGTGAAAAACAAAGGAAGAAAGAAGAGAGGAAAGCCGAGGAGACGAGGGAAAAGGAAGAAAATCTGAGAAACAAAAGGAAACAGCAGAAAGCATTGAAGATAGAGAAACAGAAAAACAAGACAAAGAGGAAATAA

Coding sequence (CDS)

ATGGCTCTGCTTCCAGGATTCCGCCATTTCATCACCCATTTTTGCAGAACCACGCCGCCAATGGCATTTCTCTTTACCCATTTCCATACCCCTATTTCCAGGCCATCCTCTCTCTTCCATATACCCAACCCCTCCAGCCCTGTTCTCCCGAAATCCCTGATAAAGTCCCACGCTTCTGGGAAAAAAACTACATTCTTTTTGACTCGGGATGAGCGATTGGAGGATTCGCATGCCGGGATTGAGCGTGAACAAATTGAGAATTCAAGCACAATTGCAGCCATCGTGACATCCATGGGGGGTCCTCCTGCTGCGGTGGGCATTGTGCGTTTATCAGGTCCTCGTGCTGTGAATATCGTTGGGAGTTTATTTTGCCCTGCCGCGAAAAAGAAGGGGAAGAATGTGAGCCAGCAGCATCCATGGAGACCCACCAGCCATGTCGTGGAGTACGGTGTGGTATTGGACCAACAGGGCGATGTTTTGACGGTGCCAATGTTAGCCCCAAGATCTTATACTCGTGAAGATGTGATCGAGATTCAGTGTCACGGAAGTGAAGTATGTCTTCGTCGTGAATTTACCCTTCGTGCATTCTTAAATGGTCGCTTAGACCTTTCACAAGCAGAAAATGTTGGGAAATTAATTTCAGCCAAGTCTACGGCTGCTGCTGATGCTGCGTTGGCTGGAATTCAGGGTGGCTTCTCTTCCTTAGTCAAATTGCTAAGAACACAATGCATCGAATTACTCACTGAAATCGAGGCCCGACTAGACTTCGATGATGAGATGCCACCGTTGAATTTGGAAACTATAATGGAGAAAGTGCATGCCATGGCACAGGAAGTAGAGACTGCTCTCGAGACAGCCAATTATGACAAGCTTCTTCAATCTGGAATACAGATAGCAATTGTTGGCCGTCCTAATGTTGGGAAGTCGAGCCTTCTTAATGCATGGAGCAAAAGCGAGAGGGCAATAGTTACAGAAATTGCTGGAACTACCAGGGATGTAATTGAAGCGAATGTTACCGTTTCTGGTATTCCTGTTACTCTTCTTGATACTGCTGGAATTAGGGAAACAGATGACATTGTGGAAAAGATAGGTGTCGAGAGATCTGAAGCTGCTGCCCTCGGAGCTGATGTCATTATTATGACTATAAGTGCCCTCGATGGATGGACTGAAGAAGATACCATACTTCTCGATAGCATACTTGCAAAAAAGAAATCAGATGGATCAACCACGCCTATACTTCTTGTCATAAACAAGATAGACTGTGCTCCATCTGCTGCAAACATGGATGCAATTAGTATCAACCGTGACTCTTTCAGTAAACAAGTTTTTACATGCGCTGTCACTGGACAAGGAATAGAGCATCTGGAGATGGCAATATCAGAGCTGATGGGACTCAACAATACTCTTGCAAGTGGACGTAGATGGACGGTAAACCAGAGACAATGCGAGCAGCTTCTTAGAACCAAGGAGGCACTTGGAAGATTGAAATCTTCGATTGAAGAGGAGCTGCCTCTTGACTTTTGGACAGTGGATTTGAGAGGTGCTGTATTAGCTCTTGGGGAAATTAGTGGTGAGGATATCTCTGAAGAGGTTTTGTCCAATATTTTTGGCAAGTTCTGTATTGAACCAATGTGTTTCAACATCAGAAGAAAGCTTACAAAAGAAGAAATGAAGGAGGAAGAGAAGAAAAGGAGAGCAAAGGAAAAGGAAAAACAGAGGAAGGAAGAAGAGAGGAAAGCCAAGGAGAAGAAGGAACGTGAAAAACAAAGGAAGAAAGAAGAGAGGAAAGCCGAGGAGACGAGGGAAAAGGAAGAAAATCTGAGAAACAAAAGGAAACAGCAGAAAGCATTGAAGATAGAGAAACAGAAAAACAAGACAAAGAGGAAATAA

Protein sequence

MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHASGKKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDVLTVPMLAPRSYTREDVIEIQCHGSEVCLRREFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCIEPMCFNIRRKLTKEEMKEEEKKRRAKEKEKQRKEEERKAKEKKEREKQRKKEERKAEETREKEENLRNKRKQQKALKIEKQKNKTKRK
BLAST of CmaCh15G007230 vs. Swiss-Prot
Match: MNME_SYNE7 (tRNA modification GTPase MnmE OS=Synechococcus elongatus (strain PCC 7942) GN=mnmE PE=3 SV=3)

HSP 1 Score: 399.8 bits (1026), Expect = 5.4e-110
Identity = 224/476 (47.06%), Postives = 310/476 (65.13%), Query Frame = 1

Query: 87  NSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHV 146
           +  TIAAI T++     +VGIVRLSG  A  I   +F          ++ Q PW   SH 
Sbjct: 5   SGDTIAAIATAIVPQQGSVGIVRLSGAAATEIARQIF---------QIAGQQPWE--SHR 64

Query: 147 VEYGVVLDQQG-----DVLTVPMLAPRSYTREDVIEIQCHGS--------EVCLRR---- 206
           + YG + D +      + L +PMLAPRSYTREDV+E+ CHG         ++C+R     
Sbjct: 65  ILYGYIRDPESGRLVDEALLLPMLAPRSYTREDVVELHCHGGLMPVQQTLQLCIRAGARL 124

Query: 207 ----EFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIE 266
               EFTLRAFLNGRLDLSQAE++  LISA+S  AA AAL  +QG     ++ LR +C++
Sbjct: 125 AEPGEFTLRAFLNGRLDLSQAESIADLISAQSPQAAQAALGSLQGKLGHPIRQLRDRCLD 184

Query: 267 LLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPN 326
           +L E+EAR+DF+D++PPL+LE I  ++ A   +++  L TA+  +LL++G++IAIVGRPN
Sbjct: 185 ILAEVEARIDFEDDLPPLDLEAIAAQLTAAGADMQAILSTADRGELLRTGLKIAIVGRPN 244

Query: 327 VGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGV 386
           VGKSSLLNAWS+ +RAIVT++ GTTRD++E+ + V GIPV +LDTAGIRET D VE+IGV
Sbjct: 245 VGKSSLLNAWSRCDRAIVTDLPGTTRDLVESQLIVGGIPVQVLDTAGIRETSDQVEQIGV 304

Query: 387 ERSEAAALGADVIIMTISALDGWTEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSA 446
           ERS  AA  AD++++TI A  GW+ ED  + +++        S  PILLVINK D   S 
Sbjct: 305 ERSRRAAQSADLVLLTIDASAGWSAEDQTIWEAV--------SDRPILLVINKRD-RLSE 364

Query: 447 ANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQL 506
           A   AI++ +  F   V+T A   QGIE LE AI   +G  +  ++   W +NQRQ   L
Sbjct: 365 AERHAIALPQQEFKAIVWTAAAQQQGIEDLEAAILAAVGTGDLTSANWDWALNQRQVAAL 424

Query: 507 LRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
              + AL R++ +++ +LPLDFWT+DLR A+ ALG I+GE+I+E +L  IF +FCI
Sbjct: 425 TTAQTALRRVEETLQAQLPLDFWTIDLREAIAALGSITGEEIAESMLDLIFSRFCI 460

BLAST of CmaCh15G007230 vs. Swiss-Prot
Match: MNME_SYNP6 (tRNA modification GTPase MnmE OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=mnmE PE=3 SV=2)

HSP 1 Score: 394.8 bits (1013), Expect = 1.7e-108
Identity = 222/476 (46.64%), Postives = 308/476 (64.71%), Query Frame = 1

Query: 87  NSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHV 146
           +  TIAAI T++     +VGIVRLSG  A  I   +F          ++ Q PW   SH 
Sbjct: 5   SGDTIAAIATAIVPQQGSVGIVRLSGAAATEIARQIF---------QIAGQQPWE--SHR 64

Query: 147 VEYGVVLDQQG-----DVLTVPMLAPRSYTREDVIEIQCHGS--------EVCLRR---- 206
           + YG + D +      + L +PMLAPRSYTREDV+E+ CHG         ++C+R     
Sbjct: 65  ILYGYIRDPESGRLVDEALLLPMLAPRSYTREDVVELHCHGGLMPVQQTLQLCIRAGARL 124

Query: 207 ----EFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIE 266
               EFTLRAFLNGRLDLSQAE++  LISA+S  AA AAL  +QG     ++ LR +C++
Sbjct: 125 AEPGEFTLRAFLNGRLDLSQAESIADLISAQSPQAAQAALGSLQGKLGHPIRQLRDRCLD 184

Query: 267 LLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPN 326
           +L E+EAR+DF+D++PPL+LE I  ++ A   +++  L TA+  +LL++G++IAIVGRPN
Sbjct: 185 ILAEVEARIDFEDDLPPLDLEAIAAQLTAAGADMQAILSTADRGELLRTGLKIAIVGRPN 244

Query: 327 VGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGV 386
           VGKSSLLNAWS+ +RAIVT++ G TRD++E+ + V GIPV +LDTAGIRET D VE+IGV
Sbjct: 245 VGKSSLLNAWSRCDRAIVTDLPGRTRDLVESQLIVGGIPVQVLDTAGIRETSDQVEQIGV 304

Query: 387 ERSEAAALGADVIIMTISALDGWTEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSA 446
           ERS  AA  AD++++TI A  GW+ ED  + +++        S  PILLVINK D   S 
Sbjct: 305 ERSRRAAQSADLVLLTIDASAGWSAEDQTIWEAV--------SDRPILLVINKRD-RLSE 364

Query: 447 ANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQL 506
           A   AI++ +  F   V+T A   +GIE LE AI   +G  +  ++   W +NQRQ   L
Sbjct: 365 AERHAIALPQQEFKAIVWTAAAQQKGIEDLEAAILAAVGTGDLTSANWDWALNQRQVAAL 424

Query: 507 LRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
              + AL R++ +++ +LPLDFWT+DLR A+ ALG I+GE I+E +L  IF +FCI
Sbjct: 425 TTAQTALRRVEETLQAQLPLDFWTIDLREAIAALGSITGEGIAESMLDLIFSRFCI 460

BLAST of CmaCh15G007230 vs. Swiss-Prot
Match: MNME_THEEB (tRNA modification GTPase MnmE OS=Thermosynechococcus elongatus (strain BP-1) GN=mnmE PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 3.4e-104
Identity = 214/473 (45.24%), Postives = 301/473 (63.64%), Query Frame = 1

Query: 90  TIAAIVTSMGGPPAAVGIVRLSGPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHVVEY 149
           TIAAI T++     ++GIVRLSG +AV I  SLF    K+         PW   SH + Y
Sbjct: 7   TIAAIATAIVPQQGSIGIVRLSGAKAVAIAQSLFEAPGKQ---------PWE--SHRILY 66

Query: 150 GVVLDQQ-----GDVLTVPMLAPRSYTREDVIEIQCHGSEVCLRR--------------- 209
           G V D Q      + L + MLAPRSYTREDV+E  CHG  + ++R               
Sbjct: 67  GYVRDPQTKERVDEALLLLMLAPRSYTREDVVEFHCHGGLIPVQRVLQLCVAAGARLADP 126

Query: 210 -EFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLT 269
            EFTLRAFLNGRLDL+QAE+V +L++A+ST AA  ALAG+ G  +  ++ +R  C+ LL 
Sbjct: 127 GEFTLRAFLNGRLDLTQAESVAELVAAQSTTAAQIALAGLTGKLARPLQQIRQTCLSLLA 186

Query: 270 EIEARLDFDDEMPPLNLETIMEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGK 329
           EIEARLDF DE+PPL+   I E++  +  +VE  L TA    L+++G+++AIVGRPNVGK
Sbjct: 187 EIEARLDFTDELPPLDPAAIAEQIRQLQHQVEAFLATAERGALIRTGLKVAIVGRPNVGK 246

Query: 330 SSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERS 389
           SSLLNAWS+S+RAIVT++ GTTRD++E+ + V GIP+ +LDTAGIRETD++VE+IGV+RS
Sbjct: 247 SSLLNAWSRSDRAIVTDLPGTTRDIVESQLVVGGIPIQVLDTAGIRETDNLVEQIGVQRS 306

Query: 390 EAAALGADVIIMTISALDGWTEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSAANM 449
             AAL AD+I++ I A  GWT  D  + D +  K++   +   +L+V+NK D       +
Sbjct: 307 RQAALSADLILLVIDASQGWTAADQAIYDQLQLKQRRQQAPQSVLVVLNKADLLSETTEV 366

Query: 450 DAISINRDSFSKQVFTCAVTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRT 509
             I +        V   A++ +GIE LE AI  L+      A+   + +NQRQ   L + 
Sbjct: 367 KDIPLPIAPI-PTVLLSALSQRGIEQLEDAILHLVQGQGVSAANLDFAINQRQAGLLEQV 426

Query: 510 KEALGRLKSSIEEELPLDFWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
            ++L  + ++I+ +LPLDFWT+DL  A  ALG ++GE+++E VL+ IF +FCI
Sbjct: 427 HQSLNHVLAAIDAQLPLDFWTIDLHAAARALGTLTGEEVTESVLTEIFSRFCI 467

BLAST of CmaCh15G007230 vs. Swiss-Prot
Match: MNME_SYNEL (tRNA modification GTPase MnmE OS=Synechococcus elongatus GN=mnmE PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 3.4e-104
Identity = 214/473 (45.24%), Postives = 301/473 (63.64%), Query Frame = 1

Query: 90  TIAAIVTSMGGPPAAVGIVRLSGPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHVVEY 149
           TIAAI T++     ++GIVRLSG +AV I  SLF    K+         PW   SH + Y
Sbjct: 7   TIAAIATAIVPQQGSIGIVRLSGAKAVAIAQSLFEAPGKQ---------PWE--SHRILY 66

Query: 150 GVVLDQQ-----GDVLTVPMLAPRSYTREDVIEIQCHGSEVCLRR--------------- 209
           G V D Q      + L + MLAPRSYTREDV+E  CHG  + ++R               
Sbjct: 67  GYVRDPQTKERVDEALLLLMLAPRSYTREDVVEFHCHGGLIPVQRVLQLCVAAGARLADP 126

Query: 210 -EFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLT 269
            EFTLRAFLNGRLDL+QAE+V +L++A+ST AA  ALAG+ G  +  ++ +R  C+ LL 
Sbjct: 127 GEFTLRAFLNGRLDLTQAESVAELVAAQSTTAAQIALAGLTGKLARPLQQIRQTCLSLLA 186

Query: 270 EIEARLDFDDEMPPLNLETIMEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGK 329
           EIEARLDF DE+PPL+   I E++  +  +VE  L TA    L+++G+++AIVGRPNVGK
Sbjct: 187 EIEARLDFTDELPPLDPAAIAEQIRQLQHQVEAFLATAERGALIRTGLKVAIVGRPNVGK 246

Query: 330 SSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERS 389
           SSLLNAWS+S+RAIVT++ GTTRD++E+ + V GIP+ +LDTAGIRETD++VE+IGV+RS
Sbjct: 247 SSLLNAWSRSDRAIVTDLPGTTRDIVESQLVVGGIPIQVLDTAGIRETDNLVEQIGVQRS 306

Query: 390 EAAALGADVIIMTISALDGWTEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSAANM 449
             AAL AD+I++ I A  GWT  D  + D +  K++   +   +L+V+NK D       +
Sbjct: 307 RQAALSADLILLVIDASQGWTAADQAIYDQLQLKQRRQQAPQSVLVVLNKADLLSETTEV 366

Query: 450 DAISINRDSFSKQVFTCAVTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRT 509
             I +        V   A++ +GIE LE AI  L+      A+   + +NQRQ   L + 
Sbjct: 367 KDIPLPIAPI-PTVLLSALSQRGIEQLEDAILHLVQGQGVSAANLDFAINQRQAGLLEQV 426

Query: 510 KEALGRLKSSIEEELPLDFWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
            ++L  + ++I+ +LPLDFWT+DL  A  ALG ++GE+++E VL+ IF +FCI
Sbjct: 427 HQSLNHVLAAIDAQLPLDFWTIDLHAAARALGTLTGEEVTESVLTEIFSRFCI 467

BLAST of CmaCh15G007230 vs. Swiss-Prot
Match: MNME_ACAM1 (tRNA modification GTPase MnmE OS=Acaryochloris marina (strain MBIC 11017) GN=mnmE PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 5.8e-104
Identity = 216/474 (45.57%), Postives = 301/474 (63.50%), Query Frame = 1

Query: 89  STIAAIVTSMGGPPAAVGIVRLSGPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHVVE 148
           +TIAAI T++     ++GIVRLSG  AV+I   LF    K++         W   SH V 
Sbjct: 4   TTIAAIATAIVPQQGSIGIVRLSGVEAVSIAKQLFQTPGKQQ---------WE--SHRVL 63

Query: 149 YGVV---LDQQ--GDVLTVPMLAPRSYTREDVIEIQCHGS--------EVCLRR------ 208
           YG +   L QQ   + L + MLAPRSYTREDV+E  CHG         E CL+       
Sbjct: 64  YGYIQQPLTQQIIDEGLLLLMLAPRSYTREDVVEFHCHGGMIAVQQVLEACLQAGAELAQ 123

Query: 209 --EFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELL 268
             EFTLRAFLNGRLDL+QAE V  L+ A+S  AA AALAG+QG  +S ++ LR +C++ L
Sbjct: 124 PGEFTLRAFLNGRLDLTQAEGVADLVGARSPQAAQAALAGVQGKLASPIRELRQRCLDTL 183

Query: 269 TEIEARLDFDDEMPPLNLETIMEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVG 328
            E+EAR+DF+D++PPL+   +  ++  +   ++  L TA+  +LL++G+ +AI+GRPNVG
Sbjct: 184 AEVEARVDFEDDLPPLDEAGVQAELQDIHATLQAILATADQGELLRNGLTVAIIGRPNVG 243

Query: 329 KSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVER 388
           KSSLLNAW + +RAIVT++ GTTRDV+E+ + V GIP+ +LDTAGIRET+D VE+IGV R
Sbjct: 244 KSSLLNAWCRCDRAIVTDLPGTTRDVVESQLVVGGIPIQVLDTAGIRETEDQVEQIGVTR 303

Query: 389 SEAAALGADVIIMTISALDGWTEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSAAN 448
           S  AA  AD++++TI A  GWT +D  L  +            P++L++NK+D  P    
Sbjct: 304 SHQAAQSADLVLLTIDASVGWTSDDQQLYQAF--------QDLPLILIVNKVDLVPQEQ- 363

Query: 449 MDAISINRDSFSKQVFTCAVTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLR 508
                +  ++ ++ V T A   QGI  LE AI E +   +  A+   W +NQRQ   L +
Sbjct: 364 ----VVYPEAIAQVVSTIAAQNQGISELETAILETVQTQSLKAANLDWAINQRQAAALQK 423

Query: 509 TKEALGRLKSSIEEELPLDFWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
            + AL  ++ +I ++LPLDFWT+DLRGA+ ALGEI+GEDI+E VL  IF +FCI
Sbjct: 424 AQAALEHVQGAIADQLPLDFWTIDLRGAIQALGEITGEDITESVLDRIFSRFCI 453

BLAST of CmaCh15G007230 vs. TrEMBL
Match: A0A0A0KTK4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G396040 PE=3 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 6.4e-259
Identity = 483/561 (86.10%), Postives = 505/561 (90.02%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHASG 60
           MALLPGFRHFI HF RTTPPMAFLFTHF TPISRPSS++ I   S+ VL KSLIKSH++G
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60

Query: 61  KKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVG 120
           K+ TF L  DERL DSHAG EREQI+NSSTIAAIVTS+GGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 SLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDV----LTVPMLAPRSYTREDVI 180
           +LF PAAKKKGKN+S  HPWRPTSHVVEYGVVLDQQGDV    LTVPMLAPRSYTREDVI
Sbjct: 121 TLFFPAAKKKGKNLSL-HPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180

Query: 181 EIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRR                EFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240

Query: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 300
           ADAALAGIQGGFSSLVK LRTQCIELLTEIEARLDFDDEMPPL+L  +MEKVHAM+QEVE
Sbjct: 241 ADAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVE 300

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV
Sbjct: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360

Query: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSIL 420
           SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIM ISALDGWT EDTILL+ IL
Sbjct: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRIL 420

Query: 421 AKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 480
           +KKKSD S TPILLVINKIDCAPS   MDA+SINRDSFSKQVFTCAVTGQGI++LEMAIS
Sbjct: 421 SKKKSDESCTPILLVINKIDCAPS-PKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAIS 480

Query: 481 ELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 540
           EL+GLN TLASGRRWTVNQRQC QLLRTKEA  RLKSSIE+ELP DFWTVDLR AVLALG
Sbjct: 481 ELVGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALG 540

Query: 541 EISGEDISEEVLSNIFGKFCI 542
           EI GEDISEE+LSNIFGKFCI
Sbjct: 541 EICGEDISEEILSNIFGKFCI 559

BLAST of CmaCh15G007230 vs. TrEMBL
Match: V4T2B8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003846mg PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 7.7e-196
Identity = 389/572 (68.01%), Postives = 439/572 (76.75%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTTPP-----MAFLFTHFHTPISRPSSL----FHIPNPSSPVLPK 60
           MALLP  R  ITH     PP     ++     FH P  R ++     F   + S P+   
Sbjct: 1   MALLPSLRSVITHQLHHRPPPPPPLLSIASFLFHKPKLRRTTCKNLSFSFSSSSKPITHN 60

Query: 61  SLIKSHASGKKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLS 120
            L    A+ K       +DERL   + G   E+     TIAAIVTS+GGPP AVGIVRLS
Sbjct: 61  PL----ATPKDNKLVFKKDERLVADN-GESEEKAATFRTIAAIVTSIGGPPGAVGIVRLS 120

Query: 121 GPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQG----DVLTVPMLAP 180
           GP AV+IVG +F P  KKK K+ S    WRPTSHVVEYGVVLD+ G    +VL VPMLAP
Sbjct: 121 GPMAVDIVGRVFKPVKKKKKKS-SGSVSWRPTSHVVEYGVVLDRHGIVVDEVLAVPMLAP 180

Query: 181 RSYTREDVIEIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQAENVGK 240
           RSYTREDV+E+QCHGSEVCLRR                EFTLRAFLNGRLDLSQAENV K
Sbjct: 181 RSYTREDVVELQCHGSEVCLRRVLRACLEAGATLAQPGEFTLRAFLNGRLDLSQAENVEK 240

Query: 241 LISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEK 300
           LISAKS AAADAALAGIQGGFSSLV  +R +CIELLTEIEARLDFDDEMPPLNL  +M+K
Sbjct: 241 LISAKSVAAADAALAGIQGGFSSLVTSVRAKCIELLTEIEARLDFDDEMPPLNLNLVMDK 300

Query: 301 VHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTR 360
           +HAM+Q+VE A+ETANYDKLLQSG+QIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTR
Sbjct: 301 IHAMSQDVENAMETANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTR 360

Query: 361 DVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEE 420
           DV+EA+VTV G+PVTLLDTAGIRETDDIVEKIGVERSEA ALGADVIIMT+SA+DGWT E
Sbjct: 361 DVVEASVTVCGVPVTLLDTAGIRETDDIVEKIGVERSEAVALGADVIIMTVSAVDGWTSE 420

Query: 421 DTILLDSILAKKKSDGSTTPILLVINKIDCAPSAANMDAISINR--DSFSKQVFTCAVTG 480
           D+ LL+ I + KKS  S+TP++LVINKIDCAPSA+N      N+  +SF+  VFTCAVTG
Sbjct: 421 DSELLNRIQSNKKSTESSTPMILVINKIDCAPSASN----EWNKVGNSFNDHVFTCAVTG 480

Query: 481 QGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWT 540
           QGI+ LE AI +++GL+   A GRRW VNQRQCEQL+RTKEAL RLKSSIEEELPLDFWT
Sbjct: 481 QGIQDLETAIMKIVGLHQIPAGGRRWAVNQRQCEQLMRTKEALVRLKSSIEEELPLDFWT 540

Query: 541 VDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
           +DLR A LALG+ISGEDISEEVLSNIFGKFCI
Sbjct: 541 IDLRDAALALGQISGEDISEEVLSNIFGKFCI 562

BLAST of CmaCh15G007230 vs. TrEMBL
Match: E0CRH3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g04970 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 8.5e-195
Identity = 384/577 (66.55%), Postives = 438/577 (75.91%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPS--SLFHIPNPSS--PVLPKSLIKS 60
           M  LP  RH I+H    +P ++       +P +R S   LF IP         P SL   
Sbjct: 1   MGHLPAIRHLISHL-HMSPRLSS-----SSPTTRSSLQPLFSIPTFQKLYSFFPPSLKPY 60

Query: 61  HASGKK-----------TTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAV 120
           H   K            TT  L +DERL       E  +  +++TIAAIVTS+GGPP+AV
Sbjct: 61  HTHSKTLVPHLHLTPNDTTLVLKKDERLGSRSVNQEHIEGTSTTTIAAIVTSLGGPPSAV 120

Query: 121 GIVRLSGPRAVNIVGSLFCPAAKKKGKNV-SQQHPWRPTSHVVEYGVVLDQQG----DVL 180
           GIVRLSGP AV IV  +F PA + KGK + S    WRPTSHVVEYGVVLD  G    +VL
Sbjct: 121 GIVRLSGPEAVAIVARVFRPARRNKGKTLGSGSGSWRPTSHVVEYGVVLDHHGNVVDEVL 180

Query: 181 TVPMLAPRSYTREDVIEIQCHGSEVCLRR----------------EFTLRAFLNGRLDLS 240
            +PMLAPRSYTREDV+E+QCHGSEVCLRR                EFTLRAFLNGRLDLS
Sbjct: 181 AIPMLAPRSYTREDVVELQCHGSEVCLRRVLRACLESGARLAEPGEFTLRAFLNGRLDLS 240

Query: 241 QAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLN 300
           QAENVGKLISAKS AAADAALAGIQGGFSSLV+ LR QCIELLTEIEARLDFDDEMPPL+
Sbjct: 241 QAENVGKLISAKSIAAADAALAGIQGGFSSLVRSLRIQCIELLTEIEARLDFDDEMPPLD 300

Query: 301 LETIMEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVT 360
           L  IM+K+H+M+Q+VE ALETANYD+LLQSG+QIAI+GRPNVGKSSLLNAWSKSERAIVT
Sbjct: 301 LNLIMDKIHSMSQDVENALETANYDQLLQSGLQIAIIGRPNVGKSSLLNAWSKSERAIVT 360

Query: 361 EIAGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISA 420
           EIAGTTRDV+EA+V++ GIPVTLLDTAGIRETDDIVEKIGVERSEA A+ ADVIIMTISA
Sbjct: 361 EIAGTTRDVVEASVSIHGIPVTLLDTAGIRETDDIVEKIGVERSEAVAISADVIIMTISA 420

Query: 421 LDGWTEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFT 480
           LDGWT +DT L + I++ KK   S+TP++LV+NKIDCAPSA     + +  +SFSK +FT
Sbjct: 421 LDGWTSQDTKLFNRIISNKKFVESSTPVILVVNKIDCAPSACT--ELFMEGNSFSKHIFT 480

Query: 481 CAVTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELP 540
           CAVTGQGI  LE AI E++GLN   A GRRWTVNQRQCEQL+RTKEAL RL SSIEEE+P
Sbjct: 481 CAVTGQGISDLESAIIEIVGLNKIPAGGRRWTVNQRQCEQLVRTKEALARLISSIEEEMP 540

Query: 541 LDFWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
           LDFWT+DLR A LALG+ISGEDISEEVL+NIFGKFCI
Sbjct: 541 LDFWTIDLREAALALGQISGEDISEEVLTNIFGKFCI 569

BLAST of CmaCh15G007230 vs. TrEMBL
Match: A0A061FGP2_THECC (TRNA modification GTPase OS=Theobroma cacao GN=TCM_034959 PE=3 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 2.7e-193
Identity = 380/568 (66.90%), Postives = 442/568 (77.82%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTT-PPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHAS 60
           MALLP  R  ITHF R T PP     +    P  + S LFH+      + P   + S  +
Sbjct: 1   MALLPSVRAIITHFYRPTKPPPRLPLSSLFFP-KKLSFLFHLSPKPQALKPLKPLAS--A 60

Query: 61  GKKTTFFLTRDERLEDSHAGIEREQIE---NSSTIAAIVTSMGGPPAAVGIVRLSGPRAV 120
            ++TTF    D+RL    +  + + I+   ++STIAAIVTS+GGPPAAVGIVRLSGP AV
Sbjct: 61  RQETTFVSNNDDRLVGFGSVNQGDAIKTQSSTSTIAAIVTSLGGPPAAVGIVRLSGPTAV 120

Query: 121 NIVGSLFCPAAKKKGKNVSQQH---PWRPTSHVVEYGVVLDQQG----DVLTVPMLAPRS 180
           +I G +F PA KKK +  S       WRPTSHVVEYGVVLD +G    +VL VPMLAP+S
Sbjct: 121 DIAGRVFFPAKKKKKQEKSFDSGSASWRPTSHVVEYGVVLDSRGNVVDEVLAVPMLAPKS 180

Query: 181 YTREDVIEIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQAENVGKLI 240
           YTREDV+E+QCHGSEVCLRR                EFTLRAFLNGRLDLSQAENVGKLI
Sbjct: 181 YTREDVVELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLI 240

Query: 241 SAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVH 300
           SAKS  AADAALAGIQGGF SLV+LLR QCIELLTEIEARLDF+DEMPPL+   +M+++H
Sbjct: 241 SAKSVGAADAALAGIQGGFCSLVRLLRGQCIELLTEIEARLDFEDEMPPLDFNLVMDRIH 300

Query: 301 AMAQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDV 360
           AM+Q+VE ALETANYDKLLQSG+QIAIVGRPNVGKSSLLNAWSKSERAIVT+IAGTTRDV
Sbjct: 301 AMSQDVEHALETANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSERAIVTDIAGTTRDV 360

Query: 361 IEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDT 420
           +EA+VTVSG+PVTLLDTAGIRETDDIVEK+GVERS A A+GADVIIMT+SALDGWT EDT
Sbjct: 361 VEASVTVSGVPVTLLDTAGIRETDDIVEKMGVERSVAVAMGADVIIMTVSALDGWTVEDT 420

Query: 421 ILLDSILAKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIE 480
            LL+ I + K+S  ++ P++L+INKIDCA SA + D +     SF+K VFTCA+TGQGI 
Sbjct: 421 KLLERIQSNKRS--TSIPMILLINKIDCASSACS-DWVDREAKSFTKHVFTCAITGQGIR 480

Query: 481 HLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLR 540
            LE +ISE++GLN   A GRRWTVNQRQCEQL+R KEA  RLKSSI+EELPLDFWT+DL+
Sbjct: 481 DLEKSISEIVGLNQIPAGGRRWTVNQRQCEQLMRAKEAFVRLKSSIKEELPLDFWTIDLK 540

Query: 541 GAVLALGEISGEDISEEVLSNIFGKFCI 542
            A LALG+ISGEDISEE+LSNIFGKFCI
Sbjct: 541 DAALALGQISGEDISEEILSNIFGKFCI 562

BLAST of CmaCh15G007230 vs. TrEMBL
Match: B9GUJ9_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s09320g PE=3 SV=2)

HSP 1 Score: 682.2 bits (1759), Expect = 6.1e-193
Identity = 379/562 (67.44%), Postives = 434/562 (77.22%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHAS- 60
           MALLP FR  ITHF +       L   F  P         + N S     K+L K HAS 
Sbjct: 1   MALLPVFRTIITHFFKPKTLNPSLSPLFQEPT--------VKNLSFLAKHKTLPKPHASL 60

Query: 61  GKKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIV 120
             KTT  L   ++      G + + I  S+TIAAIVTS+GGPPA+VGIVRLSGP AV I 
Sbjct: 61  NSKTTLLLNGQDQRFMGLNGTDDKSI--STTIAAIVTSVGGPPASVGIVRLSGPSAVEIA 120

Query: 121 GSLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQG----DVLTVPMLAPRSYTREDV 180
             +F P  KKK K       W+PTSHVV+YGVVLD QG    +VL VPMLAPRSYTREDV
Sbjct: 121 ARVFKPMRKKKKKGFCV---WKPTSHVVDYGVVLDHQGNVVDEVLAVPMLAPRSYTREDV 180

Query: 181 IEIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQAENVGKLISAKSTA 240
           +E+QCHG+EVCLRR                EFTLRAFLNGRLDLSQAENVGKLIS+KS A
Sbjct: 181 VELQCHGTEVCLRRVLRACIEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISSKSVA 240

Query: 241 AADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEV 300
           AADAALAGIQGGF+SLVK LRTQCIELLTEIEARLDFDDEMPPL+L  IM+K+H+M++ V
Sbjct: 241 AADAALAGIQGGFASLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNLIMDKIHSMSENV 300

Query: 301 ETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVT 360
           E AL+TANYDKLLQSG+QIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRD++EA++T
Sbjct: 301 ENALKTANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDIVEASIT 360

Query: 361 VSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSI 420
           V GIPV LLDTAGIR TDD+VEKIGVERSEA ALGADVI+MT+SALDGWT EDT LL+ I
Sbjct: 361 VGGIPVILLDTAGIRVTDDVVEKIGVERSEAVALGADVIVMTVSALDGWTPEDTELLNRI 420

Query: 421 LAKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAI 480
           ++KKKS GS TP++LV+NKIDC+ S  + + +     SFSK VFTCA+TGQGI+ LEMAI
Sbjct: 421 VSKKKSVGSFTPMILVVNKIDCSLSLCS-EWVDRGGGSFSKHVFTCAITGQGIQDLEMAI 480

Query: 481 SELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLAL 540
           SE++GLN   A G +WTVN RQCEQL+R KEAL RLKSSIEEE+PLDFWT+DLR A LAL
Sbjct: 481 SEIVGLNKIPAGGLKWTVNHRQCEQLVRMKEALVRLKSSIEEEMPLDFWTIDLRDAALAL 540

Query: 541 GEISGEDISEEVLSNIFGKFCI 542
           G+ISGE+ISEE+LSNIFGKFCI
Sbjct: 541 GQISGENISEEILSNIFGKFCI 548

BLAST of CmaCh15G007230 vs. TAIR10
Match: AT1G78010.1 (AT1G78010.1 tRNA modification GTPase, putative)

HSP 1 Score: 611.7 bits (1576), Expect = 5.1e-175
Identity = 349/572 (61.01%), Postives = 418/572 (73.08%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHA-S 60
           M  L  FR    +  R   P+         PIS+  +L  +   SSP    SL+K  A S
Sbjct: 1   MVSLLCFRSVGVYLLRQATPIITRGGRISRPISK--NLCFVLFSSSP--KTSLLKPRAQS 60

Query: 61  GKKTTFFLTRDERL--------EDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLS 120
            +  +     DER+        +D+   ++R Q  +SSTI AIVT +GGPP AVGIVRLS
Sbjct: 61  SENNSLVFKGDERVVGLVGKVVDDAFDKVDRFQ--SSSTIVAIVTPIGGPPGAVGIVRLS 120

Query: 121 GPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQG----DVLTVPMLAP 180
           GP+AV +   +F  +AKK  K  S    WRP SH VEYG V+D  G    +VL VPMLAP
Sbjct: 121 GPKAVEVARRVF-RSAKKTKKKESDSDTWRPKSHFVEYGAVVDSNGNVVDEVLAVPMLAP 180

Query: 181 RSYTREDVIEIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQAENVGK 240
           RSYTREDV+E+QCHGSEVCLRR                EFTLRAFLNGRLDLSQAENV K
Sbjct: 181 RSYTREDVVELQCHGSEVCLRRVLRTCVEAGARLAEPGEFTLRAFLNGRLDLSQAENVEK 240

Query: 241 LISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEK 300
           LISAKS+AAADAAL GIQGGFSSLVK LR QCIELLTEIEARLDF+DEMPPL++E+++ K
Sbjct: 241 LISAKSSAAADAALEGIQGGFSSLVKSLRAQCIELLTEIEARLDFEDEMPPLDIESVINK 300

Query: 301 VHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTR 360
           + +M+Q+VE+AL+TANYDKLLQSG+QIAIVGRPNVGKSSLLNAWSKSERAIVTE+AGTTR
Sbjct: 301 ITSMSQDVESALDTANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSERAIVTEVAGTTR 360

Query: 361 DVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEE 420
           DV+EANVTV G+P+TLLDTAGIRET+DIVEKIGVERSE AA  ADVIIM +SA++GWTEE
Sbjct: 361 DVVEANVTVRGVPITLLDTAGIRETNDIVEKIGVERSETAAKVADVIIMAVSAVEGWTEE 420

Query: 421 DTILLDSILAKKKSDGSTTPILLVINKIDCAP--SAANMDAISINRDSFSKQVFTCAVTG 480
           DT LL  I + K       P++LV+NKIDCAP  S   ++      + F K VFT AVTG
Sbjct: 421 DTELLRKIQSDK-------PMILVMNKIDCAPPGSCDQLEDQRKKEEVFHKSVFTSAVTG 480

Query: 481 QGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWT 540
           QGIE LE AI E++GL+     G +WTVNQRQCEQL+RTKEAL RL+ +IE+E+P+DFWT
Sbjct: 481 QGIEELEDAILEILGLDRVPTGGHQWTVNQRQCEQLVRTKEALVRLREAIEDEIPIDFWT 540

Query: 541 VDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
           ++LR A L+L +ISG+D+SEEVLS+IF KFCI
Sbjct: 541 IELREAALSLAQISGQDVSEEVLSSIFAKFCI 558

BLAST of CmaCh15G007230 vs. TAIR10
Match: AT3G12080.1 (AT3G12080.1 GTP-binding family protein)

HSP 1 Score: 84.3 bits (207), Expect = 2.8e-16
Identity = 60/176 (34.09%), Postives = 94/176 (53.41%), Query Frame = 1

Query: 298 IAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVT-VSGIPVTLLDTAGIRET 357
           IAI+GRPNVGKSS+LNA  + +R IV+ ++GTTRD I+A  T   G    L+DTAGIR+ 
Sbjct: 371 IAIIGRPNVGKSSILNALVREDRTIVSPVSGTTRDAIDAEFTGPDGEKFRLIDTAGIRKK 430

Query: 358 DDI------VEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSILAKKKSDGSTT 417
             +       E + V R+  A   +DV+ + I A+   TE+D  + + I  + K      
Sbjct: 431 SSVASSGSTTEAMSVNRAFRAIRRSDVVALVIEAMACITEQDLKIAERIEREGKG----- 490

Query: 418 PILLVINKIDCAPSAANMDAISINRD--------SFSKQVFTCAVTGQGIEHLEMA 459
             L+V+NK D  P+     A     D         ++  V++ A+TG  ++++ +A
Sbjct: 491 -CLVVVNKWDTIPNKNQETAAHYEDDVREKLRSLKWAPIVYSTAITGHSVDNIVVA 540

BLAST of CmaCh15G007230 vs. TAIR10
Match: AT5G39960.1 (AT5G39960.1 GTP binding;GTP binding)

HSP 1 Score: 59.7 bits (143), Expect = 7.5e-09
Identity = 60/231 (25.97%), Postives = 113/231 (48.92%), Query Frame = 1

Query: 203 LSQAENVGKLISAKSTAAA-----DAALAGIQG-GFSSLVKLLRT----QCIELLTEIEA 262
           ++++E++G L    S A A       A++   G G ++L ++LR       +E+L +I +
Sbjct: 238 MNKSESIGSLDEVASEALALGFGEPIAISAETGLGMTTLYEVLRPLLEDYTVEMLNDIGS 297

Query: 263 RLDFDDEMPPLNLETIMEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLL 322
           +   DD +   NL          + E++ +          +  +Q+AIVG+PNVGKS+LL
Sbjct: 298 Q---DDVLTDENL----------SDEIDES----------KLPLQLAIVGKPNVGKSTLL 357

Query: 323 NAWSKSERAIVTEIAGTTRDVIEANVTVSGIPVTLLDTAG---IRETDDIVEKIGVERSE 382
           NA  + ER +V   AG TRD +       G  V L+DTAG     E D     + + +S 
Sbjct: 358 NALLEEERVLVGPEAGLTRDAVRVQFEFQGRTVYLVDTAGWLERTERDKGPASLSIMQSR 417

Query: 383 AAALGADVIIMTISALDGWTEEDTILLDSILAKKKSDGSTTPILLVINKID 421
            + + A VI + + A +    + ++    ++  +++      +++++NK+D
Sbjct: 418 KSLMRAHVIALVLDAEEIIKAKCSMTHSEVVIARRAVEEGRGLVVIVNKMD 445

BLAST of CmaCh15G007230 vs. NCBI nr
Match: gi|449445112|ref|XP_004140317.1| (PREDICTED: probable tRNA modification GTPase MnmE [Cucumis sativus])

HSP 1 Score: 901.4 bits (2328), Expect = 9.2e-259
Identity = 483/561 (86.10%), Postives = 505/561 (90.02%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHASG 60
           MALLPGFRHFI HF RTTPPMAFLFTHF TPISRPSS++ I   S+ VL KSLIKSH++G
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60

Query: 61  KKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVG 120
           K+ TF L  DERL DSHAG EREQI+NSSTIAAIVTS+GGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 SLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDV----LTVPMLAPRSYTREDVI 180
           +LF PAAKKKGKN+S  HPWRPTSHVVEYGVVLDQQGDV    LTVPMLAPRSYTREDVI
Sbjct: 121 TLFFPAAKKKGKNLSL-HPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180

Query: 181 EIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRR                EFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240

Query: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 300
           ADAALAGIQGGFSSLVK LRTQCIELLTEIEARLDFDDEMPPL+L  +MEKVHAM+QEVE
Sbjct: 241 ADAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVE 300

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV
Sbjct: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360

Query: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSIL 420
           SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIM ISALDGWT EDTILL+ IL
Sbjct: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRIL 420

Query: 421 AKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 480
           +KKKSD S TPILLVINKIDCAPS   MDA+SINRDSFSKQVFTCAVTGQGI++LEMAIS
Sbjct: 421 SKKKSDESCTPILLVINKIDCAPS-PKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAIS 480

Query: 481 ELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 540
           EL+GLN TLASGRRWTVNQRQC QLLRTKEA  RLKSSIE+ELP DFWTVDLR AVLALG
Sbjct: 481 ELVGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALG 540

Query: 541 EISGEDISEEVLSNIFGKFCI 542
           EI GEDISEE+LSNIFGKFCI
Sbjct: 541 EICGEDISEEILSNIFGKFCI 559

BLAST of CmaCh15G007230 vs. NCBI nr
Match: gi|659121060|ref|XP_008460482.1| (PREDICTED: probable tRNA modification GTPase MnmE [Cucumis melo])

HSP 1 Score: 901.4 bits (2328), Expect = 9.2e-259
Identity = 481/561 (85.74%), Postives = 504/561 (89.84%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHASG 60
           MALLPGFRHFI HF RTTPPMAFL+THF TPISRPSSL+ I   S+ VL KSLIKSH++ 
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLYTHFSTPISRPSSLYSISKTSNHVLSKSLIKSHSTA 60

Query: 61  KKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVG 120
           K+ TF L  DERL DSH GIEREQIENSSTIAAIVTS+GGPPAAVGIVRLSGPRAVNIVG
Sbjct: 61  KENTFLLAPDERLADSHTGIEREQIENSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 SLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDV----LTVPMLAPRSYTREDVI 180
           +LFCPAAKKKGKN+S+ HPWRPTSHVVEYGVVLDQQGDV    LTVPMLAPRSYTREDVI
Sbjct: 121 TLFCPAAKKKGKNLSR-HPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180

Query: 181 EIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRR                EFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240

Query: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 300
           ADAALA IQGGFSSLVKLLR QCIELLTEIEARLDFDDEMPPL+L  +MEKV AM+QEVE
Sbjct: 241 ADAALAAIQGGFSSLVKLLRAQCIELLTEIEARLDFDDEMPPLDLNVVMEKVQAMSQEVE 300

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV
Sbjct: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360

Query: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSIL 420
           SGIPVTLLDTAGIRETDDIVEKIGVERSEAAA GADVIIM ISA DGWTEEDTILL+ IL
Sbjct: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGADVIIMAISAPDGWTEEDTILLNRIL 420

Query: 421 AKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 480
           +KKKSD S  PILLV+NKIDCAPS  NMDA+SINRDSFSKQVFTCAVTGQGI++LEMAIS
Sbjct: 421 SKKKSDESCIPILLVVNKIDCAPS-PNMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAIS 480

Query: 481 ELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 540
           EL+GLN TLASGRRWTVNQRQCEQLLRTKEA  RLKSSIE+ELP DFWTVDLR AVLALG
Sbjct: 481 ELVGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKSSIEDELPPDFWTVDLRAAVLALG 540

Query: 541 EISGEDISEEVLSNIFGKFCI 542
           EI GEDISEE+LSNIFGKFCI
Sbjct: 541 EICGEDISEEILSNIFGKFCI 559

BLAST of CmaCh15G007230 vs. NCBI nr
Match: gi|568837263|ref|XP_006472646.1| (PREDICTED: tRNA modification GTPase MnmE isoform X3 [Citrus sinensis])

HSP 1 Score: 697.6 bits (1799), Expect = 2.0e-197
Identity = 393/572 (68.71%), Postives = 442/572 (77.27%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTTPP-----MAFLFTHFHTPISRPSSL----FHIPNPSSPVLPK 60
           MALLP  R  ITH     PP     ++     FH P  R ++     F   + S P+   
Sbjct: 1   MALLPSLRSVITHQLHHRPPPPPPLLSIASFLFHKPKLRRTTCKNLSFSFSSSSKPITLN 60

Query: 61  SLIKSHASGKKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLS 120
            L    A+ K  T    +DERL   + G   E+    STIAAIVTS+GGPP AVGIVRLS
Sbjct: 61  PL----ATPKDNTLVFKKDERLVADN-GESEEKAGTFSTIAAIVTSIGGPPGAVGIVRLS 120

Query: 121 GPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQG----DVLTVPMLAP 180
           GP AV+IVG +F P  KKK K+ S    WRPTSHVVEYGVVLD+ G    +VL VPMLAP
Sbjct: 121 GPMAVDIVGRVFKPVKKKKKKS-SGSGSWRPTSHVVEYGVVLDRHGNVVDEVLAVPMLAP 180

Query: 181 RSYTREDVIEIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQAENVGK 240
           RSYTREDV+E+QCHGSEVCLRR                EFTLRAFLNGRLDLSQAENV K
Sbjct: 181 RSYTREDVVELQCHGSEVCLRRVLRACLEAGATFAQPGEFTLRAFLNGRLDLSQAENVEK 240

Query: 241 LISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEK 300
           LISAKS AAADAALAGIQGGFSSLV  +R +CIELLTEIEARLDFDDEMPPLNL  +M+K
Sbjct: 241 LISAKSVAAADAALAGIQGGFSSLVTSVRAKCIELLTEIEARLDFDDEMPPLNLNLVMDK 300

Query: 301 VHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTR 360
           +HAM+Q+VE ALETANYDKLLQSG+QIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTR
Sbjct: 301 IHAMSQDVENALETANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTR 360

Query: 361 DVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEE 420
           DVIEA+VTV G+PVTLLDTAGIR+TDDIVEKIGVERSEA ALGADVIIMT+SA+DGWT E
Sbjct: 361 DVIEASVTVCGVPVTLLDTAGIRKTDDIVEKIGVERSEAVALGADVIIMTVSAVDGWTSE 420

Query: 421 DTILLDSILAKKKSDGSTTPILLVINKIDCAPSAANMDAISINR--DSFSKQVFTCAVTG 480
           D+ LL+ I + KKS  S+TP++LVINKIDCAPSA+N      N+  +SF++ VFTCAVTG
Sbjct: 421 DSELLNRIQSNKKSTESSTPMILVINKIDCAPSASN----EWNKVGNSFNEHVFTCAVTG 480

Query: 481 QGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWT 540
           QGI+ LE AI E++GL+   A GRRW VNQRQCEQL+RTKEAL RLKSSIEEELPLDFWT
Sbjct: 481 QGIQDLETAIMEIVGLHQIPAGGRRWAVNQRQCEQLMRTKEALVRLKSSIEEELPLDFWT 540

Query: 541 VDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
           +DLR A LALG+ISGEDISEEVLSNIFGKFCI
Sbjct: 541 IDLRDAALALGQISGEDISEEVLSNIFGKFCI 562

BLAST of CmaCh15G007230 vs. NCBI nr
Match: gi|1009136468|ref|XP_015885541.1| (PREDICTED: tRNA modification GTPase MnmE [Ziziphus jujuba])

HSP 1 Score: 696.8 bits (1797), Expect = 3.4e-197
Identity = 392/575 (68.17%), Postives = 442/575 (76.87%), Query Frame = 1

Query: 1   MALLPGFRHFIT--HFCR-TTPPMAFLFTHF-HTPISR--------PSSLFHIPNPSSPV 60
           MA LP  R   +  H  R TT  +A L  H  ++PIS         P  L H   P S  
Sbjct: 1   MAGLPAVRFLSSSRHSYRPTTSTIASLLAHLSNSPISTSTKPSLFFPPPLCHSTKPISSK 60

Query: 61  LPKSLIKSHASGKKTTFFLTRDERLEDSHAGI--EREQIENSSTIAAIVTSMGGPPAAVG 120
           +P    K+H + ++ T    RDERL D  +G     E  +++STIAAIVTS+GGPP AVG
Sbjct: 61  IPAP--KTHLTSRENTLVFKRDERLADGGSGSLNGNELTQSASTIAAIVTSIGGPPGAVG 120

Query: 121 IVRLSGPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDV----LTV 180
           IVRLSGP AV IVG +F PA KKK KN S  + WRPTSHVVEYGVV+D  GDV    L +
Sbjct: 121 IVRLSGPSAVGIVGLVFRPAGKKKKKNCSS-YSWRPTSHVVEYGVVVDPNGDVVDEVLAI 180

Query: 181 PMLAPRSYTREDVIEIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQA 240
           PMLAPRSYTREDV+E+QCHGSEVCL R                EFTLRAFLNGRLDLSQA
Sbjct: 181 PMLAPRSYTREDVVELQCHGSEVCLNRVLRACLDAGAKLAEPGEFTLRAFLNGRLDLSQA 240

Query: 241 ENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLE 300
           ENVGKLISAKS AAADAALAGIQGGFSSLVK LR QCIELLTEIEARLDFDDEMPPL+L 
Sbjct: 241 ENVGKLISAKSVAAADAALAGIQGGFSSLVKSLRMQCIELLTEIEARLDFDDEMPPLDLG 300

Query: 301 TIMEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEI 360
            IM K+ +M+Q+VE ALETANYDKLLQSG+QIAIVGRPNVGKSSLLNAWSKSERAIVTEI
Sbjct: 301 LIMNKIDSMSQDVEHALETANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSERAIVTEI 360

Query: 361 AGTTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALD 420
           AGTTRDV+EA+VTV+GIPVTLLDTAGIRETDDIVEKIGVERSE  A+ ADVIIMT+SALD
Sbjct: 361 AGTTRDVVEASVTVNGIPVTLLDTAGIRETDDIVEKIGVERSETVAMAADVIIMTVSALD 420

Query: 421 GWTEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCA 480
           GWT ED++LL+ I + KK  GS+TP++LVINKIDC  SA  M+ +    ++FSK V TCA
Sbjct: 421 GWTTEDSLLLNRIQSNKKLTGSSTPMILVINKIDCVKSAC-MEEVHKYGNAFSKHVLTCA 480

Query: 481 VTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLD 540
           VTGQGI+ LEM ISE++GLN     GRRWTVNQRQCEQL+RTKEA   LKSSI E++PLD
Sbjct: 481 VTGQGIKELEMTISEIVGLNRVPKGGRRWTVNQRQCEQLVRTKEAFTSLKSSIAEQIPLD 540

Query: 541 FWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
           FWT+DLR A LALG+ISGEDISEEVLSNIFGKFCI
Sbjct: 541 FWTIDLRDAALALGQISGEDISEEVLSNIFGKFCI 571

BLAST of CmaCh15G007230 vs. NCBI nr
Match: gi|985440720|ref|XP_015384141.1| (PREDICTED: tRNA modification GTPase MnmE isoform X2 [Citrus sinensis])

HSP 1 Score: 696.0 bits (1795), Expect = 5.9e-197
Identity = 393/575 (68.35%), Postives = 442/575 (76.87%), Query Frame = 1

Query: 1   MALLPGFRHFITHFCRTTPP-----MAFLFTHFHTPISRPSSL----FHIPNPSSPVLPK 60
           MALLP  R  ITH     PP     ++     FH P  R ++     F   + S P+   
Sbjct: 1   MALLPSLRSVITHQLHHRPPPPPPLLSIASFLFHKPKLRRTTCKNLSFSFSSSSKPITLN 60

Query: 61  SLIKSHASGKKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLS 120
            L    A+ K  T    +DERL   + G   E+    STIAAIVTS+GGPP AVGIVRLS
Sbjct: 61  PL----ATPKDNTLVFKKDERLVADN-GESEEKAGTFSTIAAIVTSIGGPPGAVGIVRLS 120

Query: 121 GPRAVNIVGSLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDV-------LTVPM 180
           GP AV+IVG +F P  KKK K+ S    WRPTSHVVEYGVVLD+ G+V       L VPM
Sbjct: 121 GPMAVDIVGRVFKPVKKKKKKS-SGSGSWRPTSHVVEYGVVLDRHGNVVDEMGWVLAVPM 180

Query: 181 LAPRSYTREDVIEIQCHGSEVCLRR----------------EFTLRAFLNGRLDLSQAEN 240
           LAPRSYTREDV+E+QCHGSEVCLRR                EFTLRAFLNGRLDLSQAEN
Sbjct: 181 LAPRSYTREDVVELQCHGSEVCLRRVLRACLEAGATFAQPGEFTLRAFLNGRLDLSQAEN 240

Query: 241 VGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETI 300
           V KLISAKS AAADAALAGIQGGFSSLV  +R +CIELLTEIEARLDFDDEMPPLNL  +
Sbjct: 241 VEKLISAKSVAAADAALAGIQGGFSSLVTSVRAKCIELLTEIEARLDFDDEMPPLNLNLV 300

Query: 301 MEKVHAMAQEVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAG 360
           M+K+HAM+Q+VE ALETANYDKLLQSG+QIAIVGRPNVGKSSLLNAWSKSERAIVTEIAG
Sbjct: 301 MDKIHAMSQDVENALETANYDKLLQSGLQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAG 360

Query: 361 TTRDVIEANVTVSGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGW 420
           TTRDVIEA+VTV G+PVTLLDTAGIR+TDDIVEKIGVERSEA ALGADVIIMT+SA+DGW
Sbjct: 361 TTRDVIEASVTVCGVPVTLLDTAGIRKTDDIVEKIGVERSEAVALGADVIIMTVSAVDGW 420

Query: 421 TEEDTILLDSILAKKKSDGSTTPILLVINKIDCAPSAANMDAISINR--DSFSKQVFTCA 480
           T ED+ LL+ I + KKS  S+TP++LVINKIDCAPSA+N      N+  +SF++ VFTCA
Sbjct: 421 TSEDSELLNRIQSNKKSTESSTPMILVINKIDCAPSASN----EWNKVGNSFNEHVFTCA 480

Query: 481 VTGQGIEHLEMAISELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLD 540
           VTGQGI+ LE AI E++GL+   A GRRW VNQRQCEQL+RTKEAL RLKSSIEEELPLD
Sbjct: 481 VTGQGIQDLETAIMEIVGLHQIPAGGRRWAVNQRQCEQLMRTKEALVRLKSSIEEELPLD 540

Query: 541 FWTVDLRGAVLALGEISGEDISEEVLSNIFGKFCI 542
           FWT+DLR A LALG+ISGEDISEEVLSNIFGKFCI
Sbjct: 541 FWTIDLRDAALALGQISGEDISEEVLSNIFGKFCI 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MNME_SYNE75.4e-11047.06tRNA modification GTPase MnmE OS=Synechococcus elongatus (strain PCC 7942) GN=mn... [more]
MNME_SYNP61.7e-10846.64tRNA modification GTPase MnmE OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301... [more]
MNME_THEEB3.4e-10445.24tRNA modification GTPase MnmE OS=Thermosynechococcus elongatus (strain BP-1) GN=... [more]
MNME_SYNEL3.4e-10445.24tRNA modification GTPase MnmE OS=Synechococcus elongatus GN=mnmE PE=3 SV=1[more]
MNME_ACAM15.8e-10445.57tRNA modification GTPase MnmE OS=Acaryochloris marina (strain MBIC 11017) GN=mnm... [more]
Match NameE-valueIdentityDescription
A0A0A0KTK4_CUCSA6.4e-25986.10Uncharacterized protein OS=Cucumis sativus GN=Csa_5G396040 PE=3 SV=1[more]
V4T2B8_9ROSI7.7e-19668.01Uncharacterized protein OS=Citrus clementina GN=CICLE_v10003846mg PE=3 SV=1[more]
E0CRH3_VITVI8.5e-19566.55Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g04970 PE=3 SV=... [more]
A0A061FGP2_THECC2.7e-19366.90TRNA modification GTPase OS=Theobroma cacao GN=TCM_034959 PE=3 SV=1[more]
B9GUJ9_POPTR6.1e-19367.44Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s09320g PE=3 SV=2[more]
Match NameE-valueIdentityDescription
AT1G78010.15.1e-17561.01 tRNA modification GTPase, putative[more]
AT3G12080.12.8e-1634.09 GTP-binding family protein[more]
AT5G39960.17.5e-0925.97 GTP binding;GTP binding[more]
Match NameE-valueIdentityDescription
gi|449445112|ref|XP_004140317.1|9.2e-25986.10PREDICTED: probable tRNA modification GTPase MnmE [Cucumis sativus][more]
gi|659121060|ref|XP_008460482.1|9.2e-25985.74PREDICTED: probable tRNA modification GTPase MnmE [Cucumis melo][more]
gi|568837263|ref|XP_006472646.1|2.0e-19768.71PREDICTED: tRNA modification GTPase MnmE isoform X3 [Citrus sinensis][more]
gi|1009136468|ref|XP_015885541.1|3.4e-19768.17PREDICTED: tRNA modification GTPase MnmE [Ziziphus jujuba][more]
gi|985440720|ref|XP_015384141.1|5.9e-19768.35PREDICTED: tRNA modification GTPase MnmE isoform X2 [Citrus sinensis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004520GTPase_MnmE
IPR005225Small_GTP-bd_dom
IPR006073GTP_binding_domain
IPR018948GTP-bd_TrmE_N
IPR025867MnmE_helical
IPR027266TrmE/GcvT_dom1
IPR027368MnmE_dom2
IPR027417P-loop_NTPase
Vocabulary: Molecular Function
TermDefinition
GO:0003924GTPase activity
GO:0005525GTP binding
GO:0005515protein binding
Vocabulary: Cellular Component
TermDefinition
GO:0005622intracellular
Vocabulary: Biological Process
TermDefinition
GO:0006400tRNA modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
biological_process GO:0006897 endocytosis
cellular_component GO:0005622 intracellular
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G007230.1CmaCh15G007230.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004520tRNA modification GTPase MnmEHAMAPMF_00379GTPase_MnmEcoord: 89..544
score: 27
IPR004520tRNA modification GTPase MnmETIGRFAMsTIGR00450TIGR00450coord: 103..541
score: 6.9
IPR005225Small GTP-binding protein domainTIGRFAMsTIGR00231TIGR00231coord: 295..454
score: 3.9
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 297..418
score: 4.3
IPR018948GTP-binding protein TrmE, N-terminalPFAMPF10396TrmE_Ncoord: 90..188
score: 3.3
IPR025867MnmE, helical domainPFAMPF12631MnmE_helicalcoord: 203..540
score: 1.1
IPR027266GTP-binding protein TrmE/Glycine cleavage system T protein, domain 1GENE3DG3DSA:3.30.1360.120coord: 83..200
score: 1.6
IPR027368tRNA modification GTPase MnmE domain 2GENE3DG3DSA:1.20.120.430coord: 326..350
score: 2.6E-44coord: 473..541
score: 2.6E-44coord: 201..262
score: 2.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 277..325
score: 5.8E-7coord: 366..463
score: 4.
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 169..250
score: 7.96E-39coord: 283..459
score: 7.96
NoneNo IPR availableunknownCoilCoilcoord: 549..629
scor
NoneNo IPR availablePANTHERPTHR11649MSS1/TRME-RELATED GTP-BINDING PROTEINcoord: 92..541
score: 3.4E
NoneNo IPR availablePANTHERPTHR11649:SF56TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIALcoord: 92..541
score: 3.4E
NoneNo IPR availableunknownSSF103025Folate-binding domaincoord: 89..200
score: 1.41

The following gene(s) are paralogous to this gene:

None