CmaCh15G006420 (gene) Cucurbita maxima (Rimu)

NameCmaCh15G006420
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionGibberellin 20-oxidase
LocationCma_Chr15 : 3035007 .. 3036820 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCAAGTAGCCCATTGTTGGTCCTCTCCTCTCAGTTCATATGGCCTAAAGAGGATTTAGTGGACGCATATGATCAGAAGCTCACTGAGCCACACGTCGACCTTCACGGCTTCCTAAAGGGCGATCAAAAGGCCACCATCGAAGCTTCCCACCTCGTAAGGACAGCCTGCATGAAACATGGCTTCTTCCAAGTTATCAACCATGGCGTTGATCAAACCCTCCTGGCCACCGCCCTTGACAAAATGGGCCCTCTTTTTAACCTCCCTTTCCACCTTAAAACTACGGCCTCTGCCAAAATGTGGGGCTTTTCCACTGCCCATTCTAATCGCTTTTCCTCAAAGCTGCCTTGGAAGGAAACTTTCTCCTTTGGCTTTGACCATTGCAAAACCTCCAATAATGAACCCCTTGTGGTTGATTTCTTCTCTTCCATGCTCGGCCACGAATTTCAAGAAATAGGGTAAGATCAATCTATTTTAATAATGGGTTTTCTTCGTTTTACGTAAATTGTCATGAGTGTGCATTGTCGTGCGATCTTAACCGTTATTGAATAAGTAGGGCTTTATATGAGAAGTATTGCGAAGCCATGAGGGACTTGGGGCTTGCATTAACGGAGCTTTTGGGCATAAGTTTGGGCGTGGAGAGGTCGCATTTTCGCAAGTTTTTTGAGGATGGAAGCTCCATAATGCGATTGAATAGCTACCCAATATGCGAACAAGGCAGCTTGGCCCTCGGCACAGGGCCTCATTGTGATCCAACAGGCTTGACCATCCTCCACCAGGACCAGGTCGGTGGGCTTGAAGTCTTCGCCGACAACAAATGGCACACCGTTCCCCCATCTCAAAATGCACTCGTTATTAACGTGGGTGACATCTTCATGGTTAGTTCCCCAAACTAAATACAAAATCAAACCCTTTTCCTTTTCTCTCTCCTTTACATCCTTCTATTACATAATCTAACTTGCTTTTGGTCCAATACAACACAATTGAAGGACGTTAATGATTAATTATCCAAAACAAACCTATAATTTTTTTACTAGAATCATCCACCTTTACTCCCTAAAATTATTATCAGTCTCGGTTACCCGAACTTTTACTTGATAACCATTTTCTGTTATTACCTACTCGAAGCACAACCCTTATCTTGATAACCATTTTCTATAATGACTAGTAGGATAATCATTAGTGGTTGATTACCCAATACAGGTCGTGTGTAATACCATATGAAAATCAACATCCTTTTTTATTTTTTAAAATAATTATTAATGACTAGTTACCCGAAACACACTACATGTAACCCTTATCTTGATAACACATATAACATTTGCCACATGAAAATCAATATCCCTCTATTTTGCTTAAAGTGATTATTAATGGTTGGTTACTCGAAACACACTTCGTGTAACTCTTATCTTGATAACCATTTTTTGCAATCAGCACATAGAGTGACTATTAATGACCGGTTATTTGAAACACACACCGCTCATAACTCATATTCAAATTATCACTTAACCTAATTTAGGTAGAACATTTTTGTGAAGGCACTTTGTAATGGGGAATACAAAAGCTGCGTGCACCGGGCGTTGGTGAACAAACACAAGGAGAGGAGGTCGTTGGCATTTTTCCTTTGTCCGAGGAAAGACAAGGTGGTGAGGCCGCCGGAGAAACTTGTTGTCGGCGGTGGGTCGAGGAAGTATCCGGATTTTGCCTGGTCGGAGCTGCTTGAATTCACTCAGAAATATTACAGAGCCGATGCTGTAACTCTTCAAAACTTTACCAAATGGGTCGTGTCGGCGAGGCCATGTGATGTTTAG

mRNA sequence

ATGGATTCAAGTAGCCCATTGTTGGTCCTCTCCTCTCAGTTCATATGGCCTAAAGAGGATTTAGTGGACGCATATGATCAGAAGCTCACTGAGCCACACGTCGACCTTCACGGCTTCCTAAAGGGCGATCAAAAGGCCACCATCGAAGCTTCCCACCTCGTAAGGACAGCCTGCATGAAACATGGCTTCTTCCAAGTTATCAACCATGGCGTTGATCAAACCCTCCTGGCCACCGCCCTTGACAAAATGGGCCCTCTTTTTAACCTCCCTTTCCACCTTAAAACTACGGCCTCTGCCAAAATGTGGGGCTTTTCCACTGCCCATTCTAATCGCTTTTCCTCAAAGCTGCCTTGGAAGGAAACTTTCTCCTTTGGCTTTGACCATTGCAAAACCTCCAATAATGAACCCCTTGTGGTTGATTTCTTCTCTTCCATGCTCGGCCACGAATTTCAAGAAATAGGGGCTTTATATGAGAAGTATTGCGAAGCCATGAGGGACTTGGGGCTTGCATTAACGGAGCTTTTGGGCATAAGTTTGGGCGTGGAGAGGTCGCATTTTCGCAAGTTTTTTGAGGATGGAAGCTCCATAATGCGATTGAATAGCTACCCAATATGCGAACAAGGCAGCTTGGCCCTCGGCACAGGGCCTCATTGTGATCCAACAGGCTTGACCATCCTCCACCAGGACCAGGTCGGTGGGCTTGAAGTCTTCGCCGACAACAAATGGCACACCGTTCCCCCATCTCAAAATGCACTCGTTATTAACGTGGGTGACATCTTCATGGCACTTTGTAATGGGGAATACAAAAGCTGCGTGCACCGGGCGTTGGTGAACAAACACAAGGAGAGGAGGTCGTTGGCATTTTTCCTTTGTCCGAGGAAAGACAAGGTGGTGAGGCCGCCGGAGAAACTTGTTGTCGGCGGTGGGTCGAGGAAGTATCCGGATTTTGCCTGGTCGGAGCTGCTTGAATTCACTCAGAAATATTACAGAGCCGATGCTGTAACTCTTCAAAACTTTACCAAATGGGTCGTGTCGGCGAGGCCATGTGATGTTTAG

Coding sequence (CDS)

ATGGATTCAAGTAGCCCATTGTTGGTCCTCTCCTCTCAGTTCATATGGCCTAAAGAGGATTTAGTGGACGCATATGATCAGAAGCTCACTGAGCCACACGTCGACCTTCACGGCTTCCTAAAGGGCGATCAAAAGGCCACCATCGAAGCTTCCCACCTCGTAAGGACAGCCTGCATGAAACATGGCTTCTTCCAAGTTATCAACCATGGCGTTGATCAAACCCTCCTGGCCACCGCCCTTGACAAAATGGGCCCTCTTTTTAACCTCCCTTTCCACCTTAAAACTACGGCCTCTGCCAAAATGTGGGGCTTTTCCACTGCCCATTCTAATCGCTTTTCCTCAAAGCTGCCTTGGAAGGAAACTTTCTCCTTTGGCTTTGACCATTGCAAAACCTCCAATAATGAACCCCTTGTGGTTGATTTCTTCTCTTCCATGCTCGGCCACGAATTTCAAGAAATAGGGGCTTTATATGAGAAGTATTGCGAAGCCATGAGGGACTTGGGGCTTGCATTAACGGAGCTTTTGGGCATAAGTTTGGGCGTGGAGAGGTCGCATTTTCGCAAGTTTTTTGAGGATGGAAGCTCCATAATGCGATTGAATAGCTACCCAATATGCGAACAAGGCAGCTTGGCCCTCGGCACAGGGCCTCATTGTGATCCAACAGGCTTGACCATCCTCCACCAGGACCAGGTCGGTGGGCTTGAAGTCTTCGCCGACAACAAATGGCACACCGTTCCCCCATCTCAAAATGCACTCGTTATTAACGTGGGTGACATCTTCATGGCACTTTGTAATGGGGAATACAAAAGCTGCGTGCACCGGGCGTTGGTGAACAAACACAAGGAGAGGAGGTCGTTGGCATTTTTCCTTTGTCCGAGGAAAGACAAGGTGGTGAGGCCGCCGGAGAAACTTGTTGTCGGCGGTGGGTCGAGGAAGTATCCGGATTTTGCCTGGTCGGAGCTGCTTGAATTCACTCAGAAATATTACAGAGCCGATGCTGTAACTCTTCAAAACTTTACCAAATGGGTCGTGTCGGCGAGGCCATGTGATGTTTAG

Protein sequence

MDSSSPLLVLSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPCDV
BLAST of CmaCh15G006420 vs. Swiss-Prot
Match: GAOX3_ARATH (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.5e-104
Identity = 185/344 (53.78%), Postives = 235/344 (68.31%), Query Frame = 1

Query: 10  LSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 69
           +  QF+WP  +      Q L  P +DL GFL GD     EA+ LV  A  KHGFF + NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 70  GVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSN----RFSSKLPWKETFSFG 129
           GVD++LL+ A   M   F  P   K  A  K WG S+ +++    RFSSKLPWKET SF 
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRK-WGESSGYASSFVGRFSSKLPWKETLSFK 156

Query: 130 FDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSH 189
           F   +  +++  V DF S  +G  +++ G +Y++Y EAM  L L + ELLG+SLGVER +
Sbjct: 157 FSPEEKIHSQT-VKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRY 216

Query: 190 FRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTV 249
           F++FFED  SI RLN YP C+Q  LALGTGPHCDPT LTILHQDQVGGL+VF DNKW ++
Sbjct: 217 FKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSI 276

Query: 250 PPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLV 309
           PP+ +A V+N+GD FMAL NG YKSC+HRA+VN  +ER++ AFFLCP+ +KVV+PPE+LV
Sbjct: 277 PPNPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELV 336

Query: 310 VG--GGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSAR 348
            G   G RKYPDF WS  LEFTQK+YRAD  TL  F+ W+ + R
Sbjct: 337 NGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378

BLAST of CmaCh15G006420 vs. Swiss-Prot
Match: GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 9.8e-101
Identity = 176/357 (49.30%), Postives = 236/357 (66.11%), Query Frame = 1

Query: 2   DSSSPLLV----------LSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEAS 61
           D +SPL+           + +QFIWP E+       +L  P +DL       Q +T+EA 
Sbjct: 24  DQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAP 83

Query: 62  HLVRTACMKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAK---MWGFSTAH 121
            ++  AC KHGFF V+NHGV ++L+A A   M   F++P   K  A  K     G++++ 
Sbjct: 84  RVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSF 143

Query: 122 SNRFSSKLPWKETFSFGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLG 181
           + RFS+KLPWKET SF F +  + +    V D+FS  LG EF++ G +Y+ YCEAM  L 
Sbjct: 144 TGRFSTKLPWKETLSFQFSNDNSGSRT--VQDYFSDTLGQEFEQFGKVYQDYCEAMSSLS 203

Query: 182 LALTELLGISLGVERSHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQ 241
           L + ELLG+SLGV R +FR FFE+  SIMRLN YP C+   L LGTGPHCDP+ LTILHQ
Sbjct: 204 LKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQ 263

Query: 242 DQVGGLEVFADNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAF 301
           D V GL+VF DN+W ++ P+  A V+N+GD FMAL NG +KSC+HRA+VN+   R+S+AF
Sbjct: 264 DHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAF 323

Query: 302 FLCPRKDKVVRPPEKLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVS 346
           FLCP+KDKVV+PP  ++    +RKYPDF WS  LEFTQK+YRAD  TL +F+ WV++
Sbjct: 324 FLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVIT 373

BLAST of CmaCh15G006420 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 367.5 bits (942), Expect = 1.7e-100
Identity = 172/337 (51.04%), Postives = 228/337 (67.66%), Query Frame = 1

Query: 10  LSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 69
           + +QFIWP ++       +L  P +DL   L  D  +T++AS L+  AC KHGFF V+NH
Sbjct: 40  IPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLVVNH 99

Query: 70  GVDQTLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFGF 129
           G+ + L++ A +     F++P   K      S +  G++++ + RFS+KLPWKET SF F
Sbjct: 100 GISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRF 159

Query: 130 DHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHF 189
             C   +    V D+F   LGH FQ  G +Y++YCEAM  L L + ELLG+SLGV+R +F
Sbjct: 160 --CDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYF 219

Query: 190 RKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVP 249
           R+FFE+  SIMRLN YP C +  L LGTGPHCDPT LTILHQD V GL+VF +N+W ++ 
Sbjct: 220 REFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIR 279

Query: 250 PSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVV 309
           P+  A V+N+GD FMAL N  YKSC+HRA+VN   ER+SLAFFLCP+KD+VV PP +L+ 
Sbjct: 280 PNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLD 339

Query: 310 GGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWV 344
              SR+YPDF WS  LEFTQK+YRAD  TLQ F+ W+
Sbjct: 340 SITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

BLAST of CmaCh15G006420 vs. Swiss-Prot
Match: GAOX2_ORYSJ (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica GN=20ox2 PE=1 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-97
Identity = 175/333 (52.55%), Postives = 220/333 (66.07%), Query Frame = 1

Query: 14  FIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGVDQ 73
           F+WP  D   A   +L  P VD+     GD +    A+  V  AC  HGFFQV  HGVD 
Sbjct: 46  FVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHGVDA 105

Query: 74  TLLATALDKMGPLFNLPFHLKTTAS---AKMWGFSTAHSNRFSSKLPWKETFSFGFDHCK 133
            L   ALD     F LP   K  A      + G+++AH++RF+SKLPWKET SFGF H +
Sbjct: 106 ALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFGF-HDR 165

Query: 134 TSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHFRKFF 193
            +   P+V D+FSS LG +F  +G +Y+KYCE M++L L + ELL +SLGVER ++R+FF
Sbjct: 166 AA--APVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYYREFF 225

Query: 194 EDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVPPSQN 253
            D SSIMR N YP C +    LGTGPHCDPT LTIL QD VGGLEV  D +W  V P   
Sbjct: 226 ADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRPVSPVPG 285

Query: 254 ALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVVGGGS 313
           A+VIN+GD FMAL NG YKSC+HRA+VN+ +ERRSLAFFLCPR+D+VVRPP         
Sbjct: 286 AMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPPSAAT---P 345

Query: 314 RKYPDFAWSELLEFTQKYYRADAVTLQNFTKWV 344
           + YPDF W++L+ FTQ++YRAD  TL  FT+W+
Sbjct: 346 QHYPDFTWADLMRFTQRHYRADTRTLDAFTRWL 372

BLAST of CmaCh15G006420 vs. Swiss-Prot
Match: GAOX2_ORYSI (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica GN=20ox2 PE=1 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-97
Identity = 175/333 (52.55%), Postives = 220/333 (66.07%), Query Frame = 1

Query: 14  FIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGVDQ 73
           F+WP  D   A   +L  P VD+     GD +    A+  V  AC  HGFFQV  HGVD 
Sbjct: 46  FVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEHGVDA 105

Query: 74  TLLATALDKMGPLFNLPFHLKTTAS---AKMWGFSTAHSNRFSSKLPWKETFSFGFDHCK 133
            L   ALD     F LP   K  A      + G+++AH++RF+SKLPWKET SFGF H +
Sbjct: 106 ALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFGF-HDR 165

Query: 134 TSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHFRKFF 193
            +   P+V D+FSS LG +F  +G +Y+KYCE M++L L + ELL +SLGVER ++R+FF
Sbjct: 166 AA--APVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTIMELLELSLGVERGYYREFF 225

Query: 194 EDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVPPSQN 253
            D SSIMR N YP C +    LGTGPHCDPT LTIL QD VGGLEV  D +W  V P   
Sbjct: 226 ADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVDGEWRPVSPVPG 285

Query: 254 ALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVVGGGS 313
           A+VIN+GD FMAL NG YKSC+HRA+VN+ +ERRSLAFFLCPR+D+VVRPP         
Sbjct: 286 AMVINIGDTFMALSNGRYKSCLHRAVVNQRRERRSLAFFLCPREDRVVRPPPSAAT---P 345

Query: 314 RKYPDFAWSELLEFTQKYYRADAVTLQNFTKWV 344
           + YPDF W++L+ FTQ++YRAD  TL  FT+W+
Sbjct: 346 QHYPDFTWADLMRFTQRHYRADTRTLDAFTRWL 372

BLAST of CmaCh15G006420 vs. TrEMBL
Match: S0BE44_CUCSA (GA 20-oxidase OS=Cucumis sativus GN=GA20ox5 PE=2 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 4.0e-170
Identity = 283/346 (81.79%), Postives = 314/346 (90.75%), Query Frame = 1

Query: 8   LVLSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVI 67
           L + S+FIWPK DLV+AY+ KL+EPHVDL GFLKGD+KAT+EAS LVR AC+KHGFFQV 
Sbjct: 32  LFVQSKFIWPKGDLVEAYE-KLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVT 91

Query: 68  NHGVDQTLLATALDKMGPLFNLPFHLKTTAS----AKMWGFSTAHSNRFSSKLPWKETFS 127
           NHGVDQ LLATAL +MGP+FNLPF++KT AS    AKMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 92  NHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPAKMWGFSTAHSNRFSSKLPWKETFS 151

Query: 128 FGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVER 187
           FGFDHC + NNEP VVDFFSS LG EF+EIG +YEKYCEAMRDL LALTELLGISLG+ER
Sbjct: 152 FGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVIYEKYCEAMRDLSLALTELLGISLGLER 211

Query: 188 SHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWH 247
           SHFRKFFEDGSSIMRLNSYPICEQG +ALGTGPHCDPT LTILHQDQVGGLEVFA+N+WH
Sbjct: 212 SHFRKFFEDGSSIMRLNSYPICEQGGVALGTGPHCDPTALTILHQDQVGGLEVFANNQWH 271

Query: 248 TVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEK 307
           +VPP+ NALV+N+GD+FMA CNGEYKSCVHRA+VN +K+RRSLAFFLCPRK+KVVRPPEK
Sbjct: 272 SVPPTPNALVVNIGDLFMAQCNGEYKSCVHRAVVNNYKKRRSLAFFLCPRKNKVVRPPEK 331

Query: 308 LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPC 350
           LV    SRKYPDF+WSELLEFTQK+YRADA TLQNFTKWVVS+RPC
Sbjct: 332 LVADDESRKYPDFSWSELLEFTQKHYRADAATLQNFTKWVVSSRPC 376

BLAST of CmaCh15G006420 vs. TrEMBL
Match: Q4JH25_SESRO (GA 20-oxidase OS=Sesbania rostrata GN=GA20ox1 PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 1.8e-122
Identity = 209/338 (61.83%), Postives = 262/338 (77.51%), Query Frame = 1

Query: 14  FIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGVDQ 73
           FIWPKE LV+A+++ L  P VDL GFL+GD++AT  A+ L+  AC+ HGFFQVINHGVDQ
Sbjct: 36  FIWPKECLVNAHEE-LQAPLVDLDGFLRGDEEATHRAAELISKACLTHGFFQVINHGVDQ 95

Query: 74  TLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCK 133
            L+  A ++M   F LP   K +       MWG+S AH++RFSSKLPWKET SF F    
Sbjct: 96  RLIGEAYNQMDGFFKLPIDRKLSVRKIPGSMWGYSGAHADRFSSKLPWKETLSFPF---H 155

Query: 134 TSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHFRKFF 193
            +N+EP+V ++F+S LG  F++ G  ++KYCEAM+ LG+ L ELL ISL V+R H++  F
Sbjct: 156 DNNSEPVVTNYFNSTLGKTFEQTGVTFQKYCEAMKGLGMKLMELLAISLDVDRFHYKDLF 215

Query: 194 EDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVPPSQN 253
           EDG SIMR N YP C+Q SLALGTGPHCDPT LTILHQDQVGGL+VFAD KW TV P  +
Sbjct: 216 EDGCSIMRCNYYPSCQQPSLALGTGPHCDPTTLTILHQDQVGGLDVFADKKWQTVRPLPH 275

Query: 254 ALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVVGGGS 313
           ALV+N+GD F AL NG YKSC+HRA+VN++KERRSLAFF+CP++DKVVRPPE +V   G+
Sbjct: 276 ALVVNIGDTFTALSNGRYKSCLHRAVVNQYKERRSLAFFVCPKEDKVVRPPEDIVRRDGT 335

Query: 314 RKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARP 349
           ++YPDF WS+LLEFTQKYYRAD  TLQNFTKW++S++P
Sbjct: 336 KQYPDFTWSKLLEFTQKYYRADEDTLQNFTKWLLSSKP 369

BLAST of CmaCh15G006420 vs. TrEMBL
Match: A0A072V0R4_MEDTR (Gibberellin 20-oxidase OS=Medicago truncatula GN=MTR_3g088745 PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 5.4e-122
Identity = 212/357 (59.38%), Postives = 266/357 (74.51%), Query Frame = 1

Query: 1   MDSSSPLLVLSSQ------FIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLV 60
           MDSS  L  L  Q      FIWPKE L DA+++ L  P VDL GFLKGD +AT  A+ L+
Sbjct: 1   MDSSQLLCELQIQSHVPKNFIWPKEYLEDAHEE-LQAPVVDLEGFLKGDNEATQHAAMLI 60

Query: 61  RTACMKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTT---ASAKMWGFSTAHSNR 120
             AC+ HGFFQVINHGVD  L+  A  +M  +F LP H K +   A   MWG+S AH++R
Sbjct: 61  NEACLNHGFFQVINHGVDLHLITQAYVQMDTIFKLPLHRKESVYKAPGSMWGYSGAHAHR 120

Query: 121 FSSKLPWKETFSFGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLAL 180
           FSSKLPWKETFSF +     +  EP+V ++F S LG +F++ G  +EKYC AM++LG+ L
Sbjct: 121 FSSKLPWKETFSFPY---LENAVEPVVTNYFKSTLGDDFEQAGVTFEKYCNAMKELGMNL 180

Query: 181 TELLGISLGV-ERSHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQ 240
           TELL ISL V +R H+R+ FE+G SIMR N+YP C+Q SL LGTGPHCDPT +T+LHQDQ
Sbjct: 181 TELLAISLEVNDRLHYRELFEEGCSIMRCNNYPCCKQPSLVLGTGPHCDPTSITLLHQDQ 240

Query: 241 VGGLEVFADNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFL 300
           VGGL+VF DNKWHTV P  NA+V+N+GD FMAL NG YKSC+HRA+VN+HK+RRSLAFFL
Sbjct: 241 VGGLQVFVDNKWHTVQPLPNAIVVNIGDTFMALSNGRYKSCLHRAVVNQHKQRRSLAFFL 300

Query: 301 CPRKDKVVRPPEKLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSAR 348
           CP++DKVVRPP  ++   G+++YPDF WS+LLEFTQ YYRAD  TL NFT W++S++
Sbjct: 301 CPKEDKVVRPPHDIISRDGTKQYPDFTWSDLLEFTQNYYRADESTLSNFTNWLLSSK 353

BLAST of CmaCh15G006420 vs. TrEMBL
Match: B9S2R1_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0560740 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 9.1e-122
Identity = 204/338 (60.36%), Postives = 263/338 (77.81%), Query Frame = 1

Query: 10  LSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 69
           + ++F WPK+DLV A  Q+L EP VDL  FL+GD+  T++A+ +++TAC+KHGFFQV+NH
Sbjct: 16  IPTEFQWPKDDLVGA-QQELMEPVVDLEYFLRGDEGETLKAARIIKTACLKHGFFQVVNH 75

Query: 70  GVDQTLLATALDKMGPLFNLPFHLKTTAS---AKMWGFSTAHSNRFSSKLPWKETFSFGF 129
           GVD  L+  A D M   F LP   K  A     KMWG+S+AH++RF SKLPWKET SFG+
Sbjct: 76  GVDLKLIKMANDHMDNFFKLPTSQKLRARKMPGKMWGYSSAHADRFLSKLPWKETLSFGY 135

Query: 130 DHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHF 189
                + ++P+V++FF+S +G +F+++G +Y+KYCEAM++L L+L ELL ISLGV+R H+
Sbjct: 136 HE---NGSDPVVLNFFNSTMGKDFEQMGKVYQKYCEAMKELSLSLMELLAISLGVDRLHY 195

Query: 190 RKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVP 249
           +KFFED  S+MR N YPICE+ SL LGTGPHCDPT LTILHQDQVGGLEVF +++WH VP
Sbjct: 196 KKFFEDSCSVMRCNFYPICEEPSLVLGTGPHCDPTTLTILHQDQVGGLEVFTNSEWHAVP 255

Query: 250 PSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVV 309
           P   ALVINVGD F AL NG YKSC+HRA+VNK+++R+SLAFFLCP++DKVVRPP+ LV 
Sbjct: 256 PRHGALVINVGDTFTALSNGIYKSCLHRAVVNKYEDRKSLAFFLCPKEDKVVRPPQDLVC 315

Query: 310 GGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVV 345
               R YPDF WS+LL FTQ YYRAD+ TLQNFT W++
Sbjct: 316 RERKRMYPDFTWSDLLHFTQDYYRADSATLQNFTNWLL 349

BLAST of CmaCh15G006420 vs. TrEMBL
Match: F6H0G1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g01390 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.0e-121
Identity = 210/339 (61.95%), Postives = 257/339 (75.81%), Query Frame = 1

Query: 13  QFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGVD 72
           +FIW K ++  A+++ L EP VDL GF  GD+ AT  A+ LVR+AC+ HGFFQVINH VD
Sbjct: 40  EFIWAKGEVGHAHEE-LREPVVDLEGFFSGDEVATQHAAMLVRSACLNHGFFQVINHRVD 99

Query: 73  QTLLATALDKMGPLFNLPFHLKTTASAK---MWGFSTAHSNRFSSKLPWKETFSFGFDHC 132
             L+  A D M   F LP+  K  A  K   +WG+S AH++RFS KLPWKET SFGF   
Sbjct: 100 PHLITVAHDHMEAFFKLPWSKKLRAQRKPGSLWGYSGAHADRFSLKLPWKETLSFGFHE- 159

Query: 133 KTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHFRKF 192
             + +E +V DFF S LG EF++ G +Y+KYC+AM+DL L L ELL ISLGV+R H+RKF
Sbjct: 160 --NGSESVVEDFFKSTLGEEFEQTGLVYQKYCQAMKDLSLVLMELLAISLGVDRLHYRKF 219

Query: 193 FEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVPPSQ 252
           FEDGSSIMR N YP C++  LA GTGPHCDPT LTILHQDQVGGLEV+ +NKW T+ P  
Sbjct: 220 FEDGSSIMRCNYYPPCQEPGLAFGTGPHCDPTSLTILHQDQVGGLEVYVNNKWRTIRPRC 279

Query: 253 NALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVVGGG 312
           +ALVIN+GD F AL NG YKSC+HRA+VN++KERRSL FF+CPR++KVV PP+ LV   G
Sbjct: 280 DALVINLGDTFKALSNGRYKSCLHRAVVNRYKERRSLVFFVCPRENKVVTPPQDLVCREG 339

Query: 313 SRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARP 349
           +RKYPDF WS+LLEFTQKYYRAD  TLQNFTKW++S+ P
Sbjct: 340 TRKYPDFKWSDLLEFTQKYYRADDATLQNFTKWLLSSNP 374

BLAST of CmaCh15G006420 vs. TAIR10
Match: AT5G07200.1 (AT5G07200.1 gibberellin 20-oxidase 3)

HSP 1 Score: 380.2 bits (975), Expect = 1.4e-105
Identity = 185/344 (53.78%), Postives = 235/344 (68.31%), Query Frame = 1

Query: 10  LSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 69
           +  QF+WP  +      Q L  P +DL GFL GD     EA+ LV  A  KHGFF + NH
Sbjct: 37  IPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNH 96

Query: 70  GVDQTLLATALDKMGPLFNLPFHLKTTASAKMWGFSTAHSN----RFSSKLPWKETFSFG 129
           GVD++LL+ A   M   F  P   K  A  K WG S+ +++    RFSSKLPWKET SF 
Sbjct: 97  GVDESLLSRAYLHMDSFFKAPACEKQKAQRK-WGESSGYASSFVGRFSSKLPWKETLSFK 156

Query: 130 FDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSH 189
           F   +  +++  V DF S  +G  +++ G +Y++Y EAM  L L + ELLG+SLGVER +
Sbjct: 157 FSPEEKIHSQT-VKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRY 216

Query: 190 FRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTV 249
           F++FFED  SI RLN YP C+Q  LALGTGPHCDPT LTILHQDQVGGL+VF DNKW ++
Sbjct: 217 FKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSI 276

Query: 250 PPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLV 309
           PP+ +A V+N+GD FMAL NG YKSC+HRA+VN  +ER++ AFFLCP+ +KVV+PPE+LV
Sbjct: 277 PPNPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELV 336

Query: 310 VG--GGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSAR 348
            G   G RKYPDF WS  LEFTQK+YRAD  TL  F+ W+ + R
Sbjct: 337 NGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWLKNRR 378

BLAST of CmaCh15G006420 vs. TAIR10
Match: AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)

HSP 1 Score: 368.2 bits (944), Expect = 5.5e-102
Identity = 176/357 (49.30%), Postives = 236/357 (66.11%), Query Frame = 1

Query: 2   DSSSPLLV----------LSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEAS 61
           D +SPL+           + +QFIWP E+       +L  P +DL       Q +T+EA 
Sbjct: 24  DQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDSTLEAP 83

Query: 62  HLVRTACMKHGFFQVINHGVDQTLLATALDKMGPLFNLPFHLKTTASAK---MWGFSTAH 121
            ++  AC KHGFF V+NHGV ++L+A A   M   F++P   K  A  K     G++++ 
Sbjct: 84  RVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSF 143

Query: 122 SNRFSSKLPWKETFSFGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLG 181
           + RFS+KLPWKET SF F +  + +    V D+FS  LG EF++ G +Y+ YCEAM  L 
Sbjct: 144 TGRFSTKLPWKETLSFQFSNDNSGSRT--VQDYFSDTLGQEFEQFGKVYQDYCEAMSSLS 203

Query: 182 LALTELLGISLGVERSHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQ 241
           L + ELLG+SLGV R +FR FFE+  SIMRLN YP C+   L LGTGPHCDP+ LTILHQ
Sbjct: 204 LKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQ 263

Query: 242 DQVGGLEVFADNKWHTVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAF 301
           D V GL+VF DN+W ++ P+  A V+N+GD FMAL NG +KSC+HRA+VN+   R+S+AF
Sbjct: 264 DHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAF 323

Query: 302 FLCPRKDKVVRPPEKLVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVS 346
           FLCP+KDKVV+PP  ++    +RKYPDF WS  LEFTQK+YRAD  TL +F+ WV++
Sbjct: 324 FLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVIT 373

BLAST of CmaCh15G006420 vs. TAIR10
Match: AT4G25420.1 (AT4G25420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 367.5 bits (942), Expect = 9.4e-102
Identity = 172/337 (51.04%), Postives = 228/337 (67.66%), Query Frame = 1

Query: 10  LSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 69
           + +QFIWP ++       +L  P +DL   L  D  +T++AS L+  AC KHGFF V+NH
Sbjct: 40  IPNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLVVNH 99

Query: 70  GVDQTLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFGF 129
           G+ + L++ A +     F++P   K      S +  G++++ + RFS+KLPWKET SF F
Sbjct: 100 GISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRF 159

Query: 130 DHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHF 189
             C   +    V D+F   LGH FQ  G +Y++YCEAM  L L + ELLG+SLGV+R +F
Sbjct: 160 --CDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYF 219

Query: 190 RKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVP 249
           R+FFE+  SIMRLN YP C +  L LGTGPHCDPT LTILHQD V GL+VF +N+W ++ 
Sbjct: 220 REFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIR 279

Query: 250 PSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVV 309
           P+  A V+N+GD FMAL N  YKSC+HRA+VN   ER+SLAFFLCP+KD+VV PP +L+ 
Sbjct: 280 PNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLD 339

Query: 310 GGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWV 344
              SR+YPDF WS  LEFTQK+YRAD  TLQ F+ W+
Sbjct: 340 SITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

BLAST of CmaCh15G006420 vs. TAIR10
Match: AT1G60980.1 (AT1G60980.1 gibberellin 20-oxidase 4)

HSP 1 Score: 352.4 bits (903), Expect = 3.1e-97
Identity = 170/338 (50.30%), Postives = 217/338 (64.20%), Query Frame = 1

Query: 10  LSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 69
           +  +F+WP  +        L  P +DL GFL  D     EA  LV  A  KHGFF V NH
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 70  GVDQTLLATALDKMGPLFNLPFHLKTTASAKMW---GFSTAHSNRFSSKLPWKETFSFGF 129
           GVD+ LL+TA   M   F  P + K  A  K+    G++++   RF   LPWKET SF F
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 130 DHCKTSNN-EPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSH 189
              + S N    V ++ S  +G  +++ G++Y++Y E M +L L + ELLG+SLG++R H
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 190 FRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTV 249
           FR+FFED  SI RLN YP C+Q  L LGTGPHCDPT LTIL QDQV GL+VF DN+W ++
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 250 PPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLV 309
           PP   ALV+N+GD  MAL NG YKSC+HRA+VN    R++LAFFLCP+ DKVV+PP +L 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL- 335

Query: 310 VGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWV 344
              G R YPDF WS  LEFT K+YRAD  TL+ FT W+
Sbjct: 336 --EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWL 370

BLAST of CmaCh15G006420 vs. TAIR10
Match: AT1G44090.1 (AT1G44090.1 gibberellin 20-oxidase 5)

HSP 1 Score: 330.5 bits (846), Expect = 1.3e-90
Identity = 169/340 (49.71%), Postives = 218/340 (64.12%), Query Frame = 1

Query: 12  SQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGV 71
           ++F WP++D+  + +  L  P +DL GFL G++  T  A+  V+ ACM HG F V+NHG 
Sbjct: 45  AEFFWPEKDVAPS-EGDLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLVVNHGF 104

Query: 72  DQTLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFGFDH 131
              L   AL+     F L    K  A      + G++  HS RFSS LPW ET +  F  
Sbjct: 105 KSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLTLAF-- 164

Query: 132 CKTSNNEPLVV-DFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGV-ERSHF 191
                  P VV DF +S LG+  QEIG +++++C+AM  L + L ELLGIS+G+ +R+++
Sbjct: 165 ---KKGPPHVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDRTYY 224

Query: 192 RKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVP 251
           R+FFEDGS I R N YP C+Q   ALG GPH DPT +T+L QD V GLEVFA   W TV 
Sbjct: 225 RRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQTVR 284

Query: 252 PSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVV 311
           P   ALV+NVGD FMAL NG Y+SC HRA+VNK K RRSL FF CPR+DK++ PP +LV 
Sbjct: 285 PRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPELVE 344

Query: 312 G-GGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVS 346
           G   SRKYPDF W++L +FTQ  YR D  TL NF+ W+VS
Sbjct: 345 GEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWLVS 378

BLAST of CmaCh15G006420 vs. NCBI nr
Match: gi|449453097|ref|XP_004144295.1| (PREDICTED: gibberellin 20 oxidase 1-D-like [Cucumis sativus])

HSP 1 Score: 605.5 bits (1560), Expect = 5.8e-170
Identity = 283/346 (81.79%), Postives = 314/346 (90.75%), Query Frame = 1

Query: 8   LVLSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVI 67
           L + S+FIWPK DLV+AY+ KL+EPHVDL GFLKGD+KAT+EAS LVR AC+KHGFFQV 
Sbjct: 59  LFVQSKFIWPKGDLVEAYE-KLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVT 118

Query: 68  NHGVDQTLLATALDKMGPLFNLPFHLKTTAS----AKMWGFSTAHSNRFSSKLPWKETFS 127
           NHGVDQ LLATAL +MGP+FNLPF++KT AS    AKMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 119 NHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPAKMWGFSTAHSNRFSSKLPWKETFS 178

Query: 128 FGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVER 187
           FGFDHC + NNEP VVDFFSS LG EF+EIG +YEKYCEAMRDL LALTELLGISLG+ER
Sbjct: 179 FGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVIYEKYCEAMRDLSLALTELLGISLGLER 238

Query: 188 SHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWH 247
           SHFRKFFEDGSSIMRLNSYPICEQG +ALGTGPHCDPT LTILHQDQVGGLEVFA+N+WH
Sbjct: 239 SHFRKFFEDGSSIMRLNSYPICEQGGVALGTGPHCDPTALTILHQDQVGGLEVFANNQWH 298

Query: 248 TVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEK 307
           +VPP+ NALV+N+GD+FMA CNGEYKSCVHRA+VN +K+RRSLAFFLCPRK+KVVRPPEK
Sbjct: 299 SVPPTPNALVVNIGDLFMAQCNGEYKSCVHRAVVNNYKKRRSLAFFLCPRKNKVVRPPEK 358

Query: 308 LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPC 350
           LV    SRKYPDF+WSELLEFTQK+YRADA TLQNFTKWVVS+RPC
Sbjct: 359 LVADDESRKYPDFSWSELLEFTQKHYRADAATLQNFTKWVVSSRPC 403

BLAST of CmaCh15G006420 vs. NCBI nr
Match: gi|512124114|emb|CCG14216.1| (GA 20-oxidase [Cucumis sativus])

HSP 1 Score: 605.5 bits (1560), Expect = 5.8e-170
Identity = 283/346 (81.79%), Postives = 314/346 (90.75%), Query Frame = 1

Query: 8   LVLSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVI 67
           L + S+FIWPK DLV+AY+ KL+EPHVDL GFLKGD+KAT+EAS LVR AC+KHGFFQV 
Sbjct: 32  LFVQSKFIWPKGDLVEAYE-KLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVT 91

Query: 68  NHGVDQTLLATALDKMGPLFNLPFHLKTTAS----AKMWGFSTAHSNRFSSKLPWKETFS 127
           NHGVDQ LLATAL +MGP+FNLPF++KT AS    AKMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 92  NHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPAKMWGFSTAHSNRFSSKLPWKETFS 151

Query: 128 FGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVER 187
           FGFDHC + NNEP VVDFFSS LG EF+EIG +YEKYCEAMRDL LALTELLGISLG+ER
Sbjct: 152 FGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVIYEKYCEAMRDLSLALTELLGISLGLER 211

Query: 188 SHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWH 247
           SHFRKFFEDGSSIMRLNSYPICEQG +ALGTGPHCDPT LTILHQDQVGGLEVFA+N+WH
Sbjct: 212 SHFRKFFEDGSSIMRLNSYPICEQGGVALGTGPHCDPTALTILHQDQVGGLEVFANNQWH 271

Query: 248 TVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEK 307
           +VPP+ NALV+N+GD+FMA CNGEYKSCVHRA+VN +K+RRSLAFFLCPRK+KVVRPPEK
Sbjct: 272 SVPPTPNALVVNIGDLFMAQCNGEYKSCVHRAVVNNYKKRRSLAFFLCPRKNKVVRPPEK 331

Query: 308 LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARPC 350
           LV    SRKYPDF+WSELLEFTQK+YRADA TLQNFTKWVVS+RPC
Sbjct: 332 LVADDESRKYPDFSWSELLEFTQKHYRADAATLQNFTKWVVSSRPC 376

BLAST of CmaCh15G006420 vs. NCBI nr
Match: gi|659081961|ref|XP_008441598.1| (PREDICTED: gibberellin 20 oxidase 3 [Cucumis melo])

HSP 1 Score: 593.6 bits (1529), Expect = 2.3e-166
Identity = 279/345 (80.87%), Postives = 310/345 (89.86%), Query Frame = 1

Query: 8   LVLSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVI 67
           L + S+FIWPK D+V+AY  KL+EP+VDL GFLKGD+KAT+EAS LVR AC++HGFFQV 
Sbjct: 32  LFVPSKFIWPKGDIVEAYG-KLSEPYVDLEGFLKGDKKATLEASKLVRKACLEHGFFQVT 91

Query: 68  NHGVDQTLLATALDKMGPLFNLPFHLKTTAS----AKMWGFSTAHSNRFSSKLPWKETFS 127
           NHGVDQ LLATAL +MGP+FNLPF++KT AS    AKMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 92  NHGVDQNLLATALLEMGPIFNLPFNVKTRASQSHPAKMWGFSTAHSNRFSSKLPWKETFS 151

Query: 128 FGFDHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVER 187
           FGFDHC + NNEP VVDFFSS LG EF+EIG +YEKYCEAMRDL LALTELLGISLG+ER
Sbjct: 152 FGFDHCNSFNNEPSVVDFFSSALGKEFKEIGVIYEKYCEAMRDLSLALTELLGISLGLER 211

Query: 188 SHFRKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWH 247
           SHFR FFEDGSSIMRLNSYPICEQG +ALGTGPHCDPT LTILHQDQVGGLEVFA+N WH
Sbjct: 212 SHFRMFFEDGSSIMRLNSYPICEQGGVALGTGPHCDPTALTILHQDQVGGLEVFANNLWH 271

Query: 248 TVPPSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEK 307
           +VPPS NALV+N+GD+ MA CNGEYKSCVHRA+VN+HK+RRSLAFFLCPRKDKVVRPPEK
Sbjct: 272 SVPPSPNALVVNIGDLLMAQCNGEYKSCVHRAVVNQHKKRRSLAFFLCPRKDKVVRPPEK 331

Query: 308 LVVGGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARP 349
           L+    SRKYPDF+WSELLEFTQK+YRADA TLQNFTKWVVS+RP
Sbjct: 332 LISDDESRKYPDFSWSELLEFTQKHYRADAATLQNFTKWVVSSRP 375

BLAST of CmaCh15G006420 vs. NCBI nr
Match: gi|68509984|gb|AAY98356.1| (GA 20-oxidase [Sesbania rostrata])

HSP 1 Score: 447.2 bits (1149), Expect = 2.6e-122
Identity = 209/338 (61.83%), Postives = 262/338 (77.51%), Query Frame = 1

Query: 14  FIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINHGVDQ 73
           FIWPKE LV+A+++ L  P VDL GFL+GD++AT  A+ L+  AC+ HGFFQVINHGVDQ
Sbjct: 36  FIWPKECLVNAHEE-LQAPLVDLDGFLRGDEEATHRAAELISKACLTHGFFQVINHGVDQ 95

Query: 74  TLLATALDKMGPLFNLPFHLKTTA---SAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCK 133
            L+  A ++M   F LP   K +       MWG+S AH++RFSSKLPWKET SF F    
Sbjct: 96  RLIGEAYNQMDGFFKLPIDRKLSVRKIPGSMWGYSGAHADRFSSKLPWKETLSFPF---H 155

Query: 134 TSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHFRKFF 193
            +N+EP+V ++F+S LG  F++ G  ++KYCEAM+ LG+ L ELL ISL V+R H++  F
Sbjct: 156 DNNSEPVVTNYFNSTLGKTFEQTGVTFQKYCEAMKGLGMKLMELLAISLDVDRFHYKDLF 215

Query: 194 EDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVPPSQN 253
           EDG SIMR N YP C+Q SLALGTGPHCDPT LTILHQDQVGGL+VFAD KW TV P  +
Sbjct: 216 EDGCSIMRCNYYPSCQQPSLALGTGPHCDPTTLTILHQDQVGGLDVFADKKWQTVRPLPH 275

Query: 254 ALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVVGGGS 313
           ALV+N+GD F AL NG YKSC+HRA+VN++KERRSLAFF+CP++DKVVRPPE +V   G+
Sbjct: 276 ALVVNIGDTFTALSNGRYKSCLHRAVVNQYKERRSLAFFVCPKEDKVVRPPEDIVRRDGT 335

Query: 314 RKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARP 349
           ++YPDF WS+LLEFTQKYYRAD  TLQNFTKW++S++P
Sbjct: 336 KQYPDFTWSKLLEFTQKYYRADEDTLQNFTKWLLSSKP 369

BLAST of CmaCh15G006420 vs. NCBI nr
Match: gi|720042025|ref|XP_010269098.1| (PREDICTED: gibberellin 20 oxidase 1-D-like [Nelumbo nucifera])

HSP 1 Score: 446.8 bits (1148), Expect = 3.5e-122
Identity = 210/342 (61.40%), Postives = 259/342 (75.73%), Query Frame = 1

Query: 10  LSSQFIWPKEDLVDAYDQKLTEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVINH 69
           + ++F+WP+ +LV   ++ L EP VDL GFL GD++A  EA+ LVRTAC+ HGFFQV NH
Sbjct: 39  IPTEFVWPQGELVYCQEE-LREPLVDLRGFLNGDEEAIKEAAELVRTACLSHGFFQVTNH 98

Query: 70  GVDQTLLATALDKMGPLFNLPFHLKTTASAK---MWGFSTAHSNRFSSKLPWKETFSFGF 129
           GVD  L+A A D +   F LP   K     K   MWG+S +H++RFSS+LPWKET SFGF
Sbjct: 99  GVDACLIAEAHDHLDAFFKLPIGQKLRGRRKAGSMWGYSGSHADRFSSRLPWKETLSFGF 158

Query: 130 DHCKTSNNEPLVVDFFSSMLGHEFQEIGALYEKYCEAMRDLGLALTELLGISLGVERSHF 189
           D    S +  +VVD+F S+LG EF++ G +Y++YCEAM++L L + ELL ISLG++R HF
Sbjct: 159 DD--RSCDSAVVVDYFQSVLGEEFKQTGLVYQRYCEAMKELSLVIMELLAISLGIDRFHF 218

Query: 190 RKFFEDGSSIMRLNSYPICEQGSLALGTGPHCDPTGLTILHQDQVGGLEVFADNKWHTVP 249
           R+FFED SSIMR N YP CE+  L LGTGPHCDPT LTILHQDQVGGLEV A NKW TV 
Sbjct: 219 RRFFEDSSSIMRCNYYPPCEEPGLTLGTGPHCDPTSLTILHQDQVGGLEVLAQNKWQTVR 278

Query: 250 PSQNALVINVGDIFMALCNGEYKSCVHRALVNKHKERRSLAFFLCPRKDKVVRPPEKLVV 309
           P  +A VIN+GD FMAL NG YKSC HRA+VN+++ERRSLAFFLCP++DKVV PP+ LV 
Sbjct: 279 PRSDAFVINIGDTFMALSNGRYKSCQHRAVVNRYRERRSLAFFLCPKEDKVVIPPQDLVG 338

Query: 310 GGGSRKYPDFAWSELLEFTQKYYRADAVTLQNFTKWVVSARP 349
             G RKYPDF WS+LLEFTQK+YRAD  TLQNFT+W +S +P
Sbjct: 339 REGPRKYPDFTWSDLLEFTQKHYRADLKTLQNFTQWFLSNKP 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GAOX3_ARATH2.5e-10453.78Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1[more]
GAOX2_ARATH9.8e-10149.30Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1[more]
GAOX1_ARATH1.7e-10051.04Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2[more]
GAOX2_ORYSJ1.3e-9752.55Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica GN=20ox2 PE=1 SV=1[more]
GAOX2_ORYSI1.3e-9752.55Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica GN=20ox2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
S0BE44_CUCSA4.0e-17081.79GA 20-oxidase OS=Cucumis sativus GN=GA20ox5 PE=2 SV=1[more]
Q4JH25_SESRO1.8e-12261.83GA 20-oxidase OS=Sesbania rostrata GN=GA20ox1 PE=2 SV=1[more]
A0A072V0R4_MEDTR5.4e-12259.38Gibberellin 20-oxidase OS=Medicago truncatula GN=MTR_3g088745 PE=3 SV=1[more]
B9S2R1_RICCO9.1e-12260.36Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_0560740 PE=3 SV=1[more]
F6H0G1_VITVI2.0e-12161.95Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g01390 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G07200.11.4e-10553.78 gibberellin 20-oxidase 3[more]
AT5G51810.15.5e-10249.30 gibberellin 20 oxidase 2[more]
AT4G25420.19.4e-10251.04 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G60980.13.1e-9750.30 gibberellin 20-oxidase 4[more]
AT1G44090.11.3e-9049.71 gibberellin 20-oxidase 5[more]
Match NameE-valueIdentityDescription
gi|449453097|ref|XP_004144295.1|5.8e-17081.79PREDICTED: gibberellin 20 oxidase 1-D-like [Cucumis sativus][more]
gi|512124114|emb|CCG14216.1|5.8e-17081.79GA 20-oxidase [Cucumis sativus][more]
gi|659081961|ref|XP_008441598.1|2.3e-16680.87PREDICTED: gibberellin 20 oxidase 3 [Cucumis melo][more]
gi|68509984|gb|AAY98356.1|2.6e-12261.83GA 20-oxidase [Sesbania rostrata][more]
gi|720042025|ref|XP_010269098.1|3.5e-12261.40PREDICTED: gibberellin 20 oxidase 1-D-like [Nelumbo nucifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002283Isopenicillin N synthase
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0046872 metal ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G006420.1CmaCh15G006420.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002283Isopenicillin N synthasePRINTSPR00682IPNSYNTHASEcoord: 54..71
score: 2.6E-8coord: 280..298
score: 2.6E-8coord: 217..238
score: 2.
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 195..292
score: 7.6
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 193..292
score: 12
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 32..129
score: 5.8
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 11..332
score: 3.2
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 14..335
score: 1.9E
NoneNo IPR availablePANTHERPTHR10209:SF129SUBFAMILY NOT NAMEDcoord: 14..335
score: 1.9E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 11..331
score: 1.3