CmaCh14G021100 (gene) Cucurbita maxima (Rimu)

NameCmaCh14G021100
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionEukaryotic aspartyl protease family protein
LocationCma_Chr14 : 14557630 .. 14559051 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGCGAAAACCAGTCCATTTCCCTTTATCTTCTTCCTTCTCACTCTTCTCCCTCTCTCCACCGCCTTCTCCGATTTCCAAACCCTAGTCCCCAGACCTCTTCCCACTTCACCTTCCTTCTTAGCCCCGGAATCCACTGAGGGTTCCGACTCCTTCTCATCTGAGGCCACAGAATCGGAGCCTGGTTTAGCATTGCACCTTCACCATTTGGACTCCCTCTCTCTCAGCCGAACGCCGGAGGAGCTCTTCCATCTCCGCCTTCAAAGGGACGCTCTCCGAGTCAACAAGCTCAGTTTACTTGCTGCTGCCTCTCGAAATGTGAGCCGAGCGAGTGGGACTGGGTTCAGTAGCTCCGTGATCTCCGGACTCGCTCAGGGCAGCGGTGAGTACTTCACGCGCATCGGCGTCGGCACTCCGCCCAGGTATGTTTACTTGGTGCTCGACACCGGCAGCGACATAGTTTGGCTACAGTGCGCTCCTTGCAAGAATTGCTACTCTCAGACCGACCCGGTTTTCGACCCGGTTAAGTCTGGATCCTTCTCCAAGGTTCTCTGCCGGACGCCGCTGTGCGGCCGGCTCGAATCTCCGGGGTGCAACCAGCGGCAGACGTGTCTCTACCAAGTTTCTTACGGCGACGGTTCCTACACCACCGGCGAGTTCGTCACCGAAACCTTGACCTTCCGGCGCACAAAAGTGGAGCGCGTAGCCCTAGGTTGCGGCCACGATAATGAGGGATTGTTCGTTGGTGCGGCTGGGCTTTTAGGGCTGGGCCGGGGAGGGTTGTCGTTTCCTTCGCAAACCGGCCGGGCTTTCAACCAGAAATTCTCCTACTGCTTGGTGGACCGGTCCGCCTCTTCCAAACCGTCCTCCGTCGTGTTCGGCAACTCCGCCGTGTCTCGAACCGCCCGGTTCACTCCTCTCCTCACAAACCCCAGACTGGATACGTTTTACTACGTCGAACTTCTAGGGATCAGCGTTGGAGGTAGGCCCGTCTCCGGCATCTCCCCTTTACATTTCAAGCTCGATTCGACCGGTAATGGCGGAGTCATCATCGATTGCGGTACTTCTGTGACTCGGTTGAACCGACCGGCGTACATTGCCCTCCGTGACGCCTTCCGTGCTGGAGCTTCGAGTTTGAAATCGGCGGCGGAGTTTTCTCTCTTTGATACTTGCTACGACCTGTCCGGGAAGACGACGGTGAAGGTCCCAACGGTGGTGCTGCATTTCAGAAACGCCGATGTGTCGTTACCGGCGTCCAACTATCTGATCCCGGTCGACGGCAGCGGGCGATTCTGCTTCGCCTTCGCCGGAACGACCAGTGGGCTGTCGATCATCGGCAACATTCAGCAGCAAGGATTTCGGGTGGTGTACGATTTGGCAGGTTCTCGGGTGGGATTCTCTCCTCGTGGTTGCGCCTAG

mRNA sequence

ATGGTGGCGAAAACCAGTCCATTTCCCTTTATCTTCTTCCTTCTCACTCTTCTCCCTCTCTCCACCGCCTTCTCCGATTTCCAAACCCTAGTCCCCAGACCTCTTCCCACTTCACCTTCCTTCTTAGCCCCGGAATCCACTGAGGGTTCCGACTCCTTCTCATCTGAGGCCACAGAATCGGAGCCTGGTTTAGCATTGCACCTTCACCATTTGGACTCCCTCTCTCTCAGCCGAACGCCGGAGGAGCTCTTCCATCTCCGCCTTCAAAGGGACGCTCTCCGAGTCAACAAGCTCAGTTTACTTGCTGCTGCCTCTCGAAATGTGAGCCGAGCGAGTGGGACTGGGTTCAGTAGCTCCGTGATCTCCGGACTCGCTCAGGGCAGCGGTGAGTACTTCACGCGCATCGGCGTCGGCACTCCGCCCAGGTATGTTTACTTGGTGCTCGACACCGGCAGCGACATAGTTTGGCTACAGTGCGCTCCTTGCAAGAATTGCTACTCTCAGACCGACCCGGTTTTCGACCCGGTTAAGTCTGGATCCTTCTCCAAGGTTCTCTGCCGGACGCCGCTGTGCGGCCGGCTCGAATCTCCGGGGTGCAACCAGCGGCAGACGTGTCTCTACCAAGTTTCTTACGGCGACGGTTCCTACACCACCGGCGAGTTCGTCACCGAAACCTTGACCTTCCGGCGCACAAAAGTGGAGCGCGTAGCCCTAGGTTGCGGCCACGATAATGAGGGATTGTTCGTTGGTGCGGCTGGGCTTTTAGGGCTGGGCCGGGGAGGGTTGTCGTTTCCTTCGCAAACCGGCCGGGCTTTCAACCAGAAATTCTCCTACTGCTTGGTGGACCGGTCCGCCTCTTCCAAACCGTCCTCCGTCGTGTTCGGCAACTCCGCCGTGTCTCGAACCGCCCGGTTCACTCCTCTCCTCACAAACCCCAGACTGGATACGTTTTACTACGTCGAACTTCTAGGGATCAGCGTTGGAGGTAGGCCCGTCTCCGGCATCTCCCCTTTACATTTCAAGCTCGATTCGACCGGTAATGGCGGAGTCATCATCGATTGCGGTACTTCTGTGACTCGGTTGAACCGACCGGCGTACATTGCCCTCCGTGACGCCTTCCGTGCTGGAGCTTCGAGTTTGAAATCGGCGGCGGAGTTTTCTCTCTTTGATACTTGCTACGACCTGTCCGGGAAGACGACGGTGAAGGTCCCAACGGTGGTGCTGCATTTCAGAAACGCCGATGTGTCGTTACCGGCGTCCAACTATCTGATCCCGGTCGACGGCAGCGGGCGATTCTGCTTCGCCTTCGCCGGAACGACCAGTGGGCTGTCGATCATCGGCAACATTCAGCAGCAAGGATTTCGGGTGGTGTACGATTTGGCAGGTTCTCGGGTGGGATTCTCTCCTCGTGGTTGCGCCTAG

Coding sequence (CDS)

ATGGTGGCGAAAACCAGTCCATTTCCCTTTATCTTCTTCCTTCTCACTCTTCTCCCTCTCTCCACCGCCTTCTCCGATTTCCAAACCCTAGTCCCCAGACCTCTTCCCACTTCACCTTCCTTCTTAGCCCCGGAATCCACTGAGGGTTCCGACTCCTTCTCATCTGAGGCCACAGAATCGGAGCCTGGTTTAGCATTGCACCTTCACCATTTGGACTCCCTCTCTCTCAGCCGAACGCCGGAGGAGCTCTTCCATCTCCGCCTTCAAAGGGACGCTCTCCGAGTCAACAAGCTCAGTTTACTTGCTGCTGCCTCTCGAAATGTGAGCCGAGCGAGTGGGACTGGGTTCAGTAGCTCCGTGATCTCCGGACTCGCTCAGGGCAGCGGTGAGTACTTCACGCGCATCGGCGTCGGCACTCCGCCCAGGTATGTTTACTTGGTGCTCGACACCGGCAGCGACATAGTTTGGCTACAGTGCGCTCCTTGCAAGAATTGCTACTCTCAGACCGACCCGGTTTTCGACCCGGTTAAGTCTGGATCCTTCTCCAAGGTTCTCTGCCGGACGCCGCTGTGCGGCCGGCTCGAATCTCCGGGGTGCAACCAGCGGCAGACGTGTCTCTACCAAGTTTCTTACGGCGACGGTTCCTACACCACCGGCGAGTTCGTCACCGAAACCTTGACCTTCCGGCGCACAAAAGTGGAGCGCGTAGCCCTAGGTTGCGGCCACGATAATGAGGGATTGTTCGTTGGTGCGGCTGGGCTTTTAGGGCTGGGCCGGGGAGGGTTGTCGTTTCCTTCGCAAACCGGCCGGGCTTTCAACCAGAAATTCTCCTACTGCTTGGTGGACCGGTCCGCCTCTTCCAAACCGTCCTCCGTCGTGTTCGGCAACTCCGCCGTGTCTCGAACCGCCCGGTTCACTCCTCTCCTCACAAACCCCAGACTGGATACGTTTTACTACGTCGAACTTCTAGGGATCAGCGTTGGAGGTAGGCCCGTCTCCGGCATCTCCCCTTTACATTTCAAGCTCGATTCGACCGGTAATGGCGGAGTCATCATCGATTGCGGTACTTCTGTGACTCGGTTGAACCGACCGGCGTACATTGCCCTCCGTGACGCCTTCCGTGCTGGAGCTTCGAGTTTGAAATCGGCGGCGGAGTTTTCTCTCTTTGATACTTGCTACGACCTGTCCGGGAAGACGACGGTGAAGGTCCCAACGGTGGTGCTGCATTTCAGAAACGCCGATGTGTCGTTACCGGCGTCCAACTATCTGATCCCGGTCGACGGCAGCGGGCGATTCTGCTTCGCCTTCGCCGGAACGACCAGTGGGCTGTCGATCATCGGCAACATTCAGCAGCAAGGATTTCGGGTGGTGTACGATTTGGCAGGTTCTCGGGTGGGATTCTCTCCTCGTGGTTGCGCCTAG

Protein sequence

MVAKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATESEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGTGFSSSVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
BLAST of CmaCh14G021100 vs. Swiss-Prot
Match: APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 1.2e-178
Identity = 329/483 (68.12%), Postives = 377/483 (78.05%), Query Frame = 1

Query: 8   FPFIFFLLTLLPLSTAFSDFQTLVP-------------RPLPTSPSFLAPESTEGSDSFS 67
           F   FF L+L P  ++   FQTL P             +P   S S L  E   GSDS  
Sbjct: 10  FSLCFFFLSL-PSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDS-- 69

Query: 68  SEATESEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAA--SRNVSRAS 127
               ES   + L+L H+D+LS ++TP+ELF  RLQRD+ RV  ++ LAA    RNV+ A 
Sbjct: 70  ----ESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP 129

Query: 128 GTG-FSSSVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDP 187
             G FSSSV+SGL+QGSGEYFTR+GVGTP RYVY+VLDTGSDIVWLQCAPC+ CYSQ+DP
Sbjct: 130 RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDP 189

Query: 188 VFDPVKSGSFSKVLCRTPLCGRLESPGCN-QRQTCLYQVSYGDGSYTTGEFVTETLTFRR 247
           +FDP KS +++ + C +P C RL+S GCN +R+TCLYQVSYGDGS+T G+F TETLTFRR
Sbjct: 190 IFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRR 249

Query: 248 TKVERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPS 307
            +V+ VALGCGHDNEGLFVGAAGLLGLG+G LSFP QTG  FNQKFSYCLVDRSASSKPS
Sbjct: 250 NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPS 309

Query: 308 SVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGV 367
           SVVFGN+AVSR ARFTPLL+NP+LDTFYYV LLGISVGG  V G++   FKLD  GNGGV
Sbjct: 310 SVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGV 369

Query: 368 IIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHF 427
           IID GTSVTRL RPAYIA+RDAFR GA +LK A +FSLFDTC+DLS    VKVPTVVLHF
Sbjct: 370 IIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF 429

Query: 428 RNADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPR 474
           R ADVSLPA+NYLIPVD +G+FCFAFAGT  GLSIIGNIQQQGFRVVYDLA SRVGF+P 
Sbjct: 430 RGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPG 485

BLAST of CmaCh14G021100 vs. Swiss-Prot
Match: ASPG2_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.7e-108
Identity = 222/473 (46.93%), Postives = 293/473 (61.95%), Query Frame = 1

Query: 10  FIFFLLTLLPLSTA----FSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATESEPGLA 69
           F FFL   L LS++    F DFQ +     P + +   P+    +  FS E++       
Sbjct: 6   FFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFN--NTHFSDESSSKYTLRL 65

Query: 70  LHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNV-----SRASGTGFSSSV 129
           LH     S++  R      H R++RD  RV+  ++L   S  V     SR     F S +
Sbjct: 66  LHRDRFPSVTY-RNHHHRLHARMRRDTDRVS--AILRRISGKVIPSSDSRYEVNDFGSDI 125

Query: 130 ISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGS 189
           +SG+ QGSGEYF RIGVG+PPR  Y+V+D+GSD+VW+QC PCK CY Q+DPVFDP KSGS
Sbjct: 126 VSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGS 185

Query: 190 FSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGC 249
           ++ V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA+GC
Sbjct: 186 YTGVSCGSSVCDRIENSGCHSGG-CRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGC 245

Query: 250 GHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAVS 309
           GH N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  A+ 
Sbjct: 246 GHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREALP 305

Query: 310 RTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTR 369
             A + PL+ NPR  +FYYV L G+ VGG  +  +    F L  TG+GGV++D GT+VTR
Sbjct: 306 VGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTAVTR 365

Query: 370 LNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADV-SLPA 429
           L   AY+A RD F++  ++L  A+  S+FDTCYDLSG  +V+VPTV  +F    V +LPA
Sbjct: 366 LPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPA 425

Query: 430 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 473
            N+L+PVD SG +CFAFA + +GLSIIGNIQQ+G +V +D A   VGF P  C
Sbjct: 426 RNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of CmaCh14G021100 vs. Swiss-Prot
Match: ASPG1_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.2e-106
Identity = 225/475 (47.37%), Postives = 291/475 (61.26%), Query Frame = 1

Query: 17  LLPLSTAFSDFQTLVPR-PLPTSPSFLAPESTEGSDSFSSEATESEPGLALHLHHLDSLS 76
           +L + ++    QT++   P  +S +   PES      F+S +      L+L LH  D+  
Sbjct: 37  VLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSDPVFFNSSSP-----LSLELHSRDTFV 96

Query: 77  LSRTPE--ELFHLRLQRDALRVNKL-------------SLLAAASRNVSRASGTGFSSSV 136
            S+  +   L   RL+RD+ RV  +             S L       +R      ++ V
Sbjct: 97  ASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPV 156

Query: 137 ISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGS 196
           +SG +QGSGEYF+RIGVGTP + +YLVLDTGSD+ W+QC PC +CY Q+DPVF+P  S +
Sbjct: 157 VSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSST 216

Query: 197 FSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRT-KVERVALG 256
           +  + C  P C  LE+  C   + CLYQVSYGDGS+T GE  T+T+TF  + K+  VALG
Sbjct: 217 YKSLTCSAPQCSLLETSACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALG 276

Query: 257 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAV 316
           CGHDNEGLF GAAGLLGLG G LS  +Q        FSYCLVDR  S K SS+ F +  +
Sbjct: 277 CGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQL 336

Query: 317 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 376
                  PLL N ++DTFYYV L G SVGG  V  +    F +D++G+GGVI+DCGT+VT
Sbjct: 337 GGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVV-LPDAIFDVDASGSGGVILDCGTAVT 396

Query: 377 RLNRPAYIALRDAF-RAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNA-DVSL 436
           RL   AY +LRDAF +   +  K ++  SLFDTCYD S  +TVKVPTV  HF     + L
Sbjct: 397 RLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDL 456

Query: 437 PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 473
           PA NYLIPVD SG FCFAFA T+S LSIIGN+QQQG R+ YDL+ + +G S   C
Sbjct: 457 PAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CmaCh14G021100 vs. Swiss-Prot
Match: ASPA_ARATH (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 3.6e-74
Identity = 165/406 (40.64%), Postives = 229/406 (56.40%), Query Frame = 1

Query: 79  TPEELFHLRLQRDALRVNKLSLLAA---ASRNVSRASGTGFSSSVISGLAQGSGEYFTRI 138
           +P+ +  LRL  D  RVN +    +   A+ +VS +  T   +    G   GSG Y   +
Sbjct: 81  SPDHVEILRL--DQARVNSIHSKLSKKLATDHVSESKSTDLPAK--DGSTLGSGNYIVTV 140

Query: 139 GVGTPPRYVYLVLDTGSDIVWLQCAPC-KNCYSQTDPVFDPVKSGSFSKVLCRTPLCGRL 198
           G+GTP   + L+ DTGSD+ W QC PC + CY Q +P+F+P KS S+  V C +  CG L
Sbjct: 141 GLGTPKNDLSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSL 200

Query: 199 ESP----GCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKV-ERVALGCGHDNEGLFV 258
            S     G      C+Y + YGD S++ G    E  T   + V + V  GCG +N+GLF 
Sbjct: 201 SSATGNAGSCSASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFT 260

Query: 259 GAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLL 318
           G AGLLGLGR  LSFPSQT  A+N+ FSYCL   S++S    + FG++ +SR+ +FTP+ 
Sbjct: 261 GVAGLLGLGRDKLSFPSQTATAYNKIFSYCL--PSSASYTGHLTFGSAGISRSVKFTPIS 320

Query: 319 TNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTRLNRPAYIAL 378
           T     +FY + ++ I+VGG+ +    P+   + ST   G +ID GT +TRL   AY AL
Sbjct: 321 TITDGTSFYGLNIVAITVGGQKL----PIPSTVFST--PGALIDSGTVITRLPPKAYAAL 380

Query: 379 RDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPASNYLIPVDGS 438
           R +F+A  S   + +  S+ DTC+DLSG  TV +P V   F    V    S  +  V   
Sbjct: 381 RSSFKAKMSKYPTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKI 440

Query: 439 GRFCFAFAGTT--SGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
            + C AFAG +  S  +I GN+QQQ   VVYD AG RVGF+P GC+
Sbjct: 441 SQVCLAFAGNSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGCS 474

BLAST of CmaCh14G021100 vs. Swiss-Prot
Match: AED1_ARATH (Aspartyl protease AED1 OS=Arabidopsis thaliana GN=AED1 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.1e-73
Identity = 173/425 (40.71%), Postives = 237/425 (55.76%), Query Frame = 1

Query: 54  SSEATESEPGL-ALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKL-SLLAAASRN-VSR 113
           SS+A+ ++  L  +H+H   S  LS          ++RD  RV  + S L+  S N VS 
Sbjct: 55  SSKASNTKSSLRVVHMHGACS-HLSSDARVDHDEIIRRDQARVESIYSKLSKNSANEVSE 114

Query: 114 ASGTGFSSSVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCK-NCYSQT 173
           A  T   +   SG+  GSG Y   IG+GTP   + LV DTGSD+ W QC PC  +CYSQ 
Sbjct: 115 AKSTELPAK--SGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQK 174

Query: 174 DPVFDPVKSGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFR 233
           +P F+P  S ++  V C +P+C   ES   +    C+Y + YGD S+T G    E  T  
Sbjct: 175 EPKFNPSSSSTYQNVSCSSPMCEDAESCSASN---CVYSIVYGDKSFTQGFLAKEKFTLT 234

Query: 234 RTKV-ERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSK 293
            + V E V  GCG +N+GLF G AGLLGLG G LS P+QT   +N  FSYCL   +++S 
Sbjct: 235 NSDVLEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNS- 294

Query: 294 PSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNG 353
              + FG++ +S + +FTP+ + P     Y ++++GISVG + ++ I+P  F  +     
Sbjct: 295 TGHLTFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELA-ITPNSFSTE----- 354

Query: 354 GVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVL 413
           G IID GT  TRL    Y  LR  F+   SS KS + + LFDTCYD +G  TV  PT+  
Sbjct: 355 GAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAF 414

Query: 414 HFRNAD-VSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGF 473
            F  +  V L  S   +P+  S + C AFAG     +I GN+QQ    VVYD+AG RVGF
Sbjct: 415 SFAGSTVVELDGSGISLPIKIS-QVCLAFAGNDDLPAIFGNVQQTTLDVVYDVAGGRVGF 464

BLAST of CmaCh14G021100 vs. TrEMBL
Match: A0A0A0L8K0_CUCSA (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus GN=Csa_3G119540 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 8.3e-235
Identity = 417/474 (87.97%), Postives = 435/474 (91.77%), Query Frame = 1

Query: 1   MVAKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATES 60
           M   T   PFIFFLLT+L L+TAFSDFQTL    LP+SPSFL  +S   +   SSEAT+S
Sbjct: 42  MEPNTISLPFIFFLLTVLSLATAFSDFQTLPLTSLPSSPSFLPSDS---NSFLSSEATQS 101

Query: 61  EPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGT-GFSSS 120
           E GL LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A SRN+S+  GT GFSSS
Sbjct: 102 ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSKPGGTTGFSSS 161

Query: 121 VISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180
           VISGLAQGSGEYFTRIGVGTPP+YVY+VLDTGSDIVWLQCAPCKNCYSQTDPVF+PVKSG
Sbjct: 162 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 221

Query: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SF+KVLCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VALG
Sbjct: 222 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG 281

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GR FNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 282 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 341

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGI+  HFKLD TGNGGVIIDCGTSVT
Sbjct: 342 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGITASHFKLDRTGNGGVIIDCGTSVT 401

Query: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420
           RLN+PAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLPA
Sbjct: 402 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 461

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 462 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 512

BLAST of CmaCh14G021100 vs. TrEMBL
Match: W9R017_9ROSA (Aspartic proteinase nepenthesin-1 OS=Morus notabilis GN=L484_017553 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 9.6e-191
Identity = 350/475 (73.68%), Postives = 390/475 (82.11%), Query Frame = 1

Query: 3   AKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATESEP 62
           A T P  +   LLT LP+       QTL     P S S L     E S++ ++E TE+  
Sbjct: 26  ASTPPLEYETLLLTSLPIPQ-----QTL---SWPDSESELTGSDLE-SETAAAEETETSL 85

Query: 63  GLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLL--AAASRNVSRASGT--GFSS 122
            ++  LHH+D+LS  ++PE+LF LRLQRDALRV  L  +  AAASRNVSR  G   GFSS
Sbjct: 86  SISAQLHHIDALSADKSPEQLFDLRLQRDALRVKNLVEVTAAAASRNVSRTRGAAPGFSS 145

Query: 123 SVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKS 182
           SVISGLAQGSGEYFTR+GVGTPPRYVY+VLDTGSD+VWLQCAPC+ CY+Q DPVFDP KS
Sbjct: 146 SVISGLAQGSGEYFTRLGVGTPPRYVYMVLDTGSDVVWLQCAPCRKCYTQADPVFDPSKS 205

Query: 183 GSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVAL 242
            SF+++ C +PLC +L+SPGCNQR+ CLYQVSYGDGS+TTGEF TETLTFRRT++ RVAL
Sbjct: 206 RSFARISCGSPLCRKLDSPGCNQRKMCLYQVSYGDGSFTTGEFSTETLTFRRTRIGRVAL 265

Query: 243 GCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSA 302
           GCGHDNEGLFVGAAGLLGLGRG LSFP QTG  FN+KFSYCL DRSASSKPSS+VFG+SA
Sbjct: 266 GCGHDNEGLFVGAAGLLGLGRGRLSFPFQTGLRFNRKFSYCLADRSASSKPSSMVFGDSA 325

Query: 303 VSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSV 362
           VSRTARFTPLLTNP+LDTFYY+ELL ISVGG  V GIS   FKLD  GNGGVIID GTSV
Sbjct: 326 VSRTARFTPLLTNPKLDTFYYLELLAISVGGSRVRGISASLFKLDQAGNGGVIIDSGTSV 385

Query: 363 TRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLP 422
           TRL RPAY+ALRDAFRAG+ +LK A EFSLFDTCYDLSGKT VKVPTVVLHFR ADVS P
Sbjct: 386 TRLTRPAYVALRDAFRAGSVNLKRAPEFSLFDTCYDLSGKTEVKVPTVVLHFRGADVSFP 445

Query: 423 ASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           A+NYLIPVD SG FCFAFAGT SGLSIIGNIQQQGFRVVYDLAGSRVGF+PRGCA
Sbjct: 446 ATNYLIPVDSSGTFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRVGFAPRGCA 491

BLAST of CmaCh14G021100 vs. TrEMBL
Match: B9SBG8_RICCO (Aspartic proteinase nepenthesin-1, putative OS=Ricinus communis GN=RCOM_0717990 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.2e-190
Identity = 345/464 (74.35%), Postives = 384/464 (82.76%), Query Frame = 1

Query: 11  IFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATESEPGLALHLHH 70
           +FF  T+    +   ++QTLV  PL + P+    +S   +D+  S AT S     + LHH
Sbjct: 12  LFFSFTIFFSHSTSLNYQTLVANPLRSQPTLSWTDSESPTDTAESSATFS-----VQLHH 71

Query: 71  LDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGTGFSSSVISGLAQGSGE 130
           +D+LS + TPE LF  RLQRDA RV  +S LA  +    R  GTGFSSSVISGLAQGSGE
Sbjct: 72  VDALSFNSTPETLFTTRLQRDAARVEAISYLAETAGTGKRV-GTGFSSSVISGLAQGSGE 131

Query: 131 YFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGSFSKVLCRTPL 190
           YFTRIGVGTPPRYVY+VLDTGSDIVW+QCAPCK CY+Q+DPVFDP KS SF+ + CR+PL
Sbjct: 132 YFTRIGVGTPPRYVYMVLDTGSDIVWIQCAPCKRCYAQSDPVFDPRKSRSFASIACRSPL 191

Query: 191 CGRLESPGCN-QRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCGHDNEGLFV 250
           C RL+SPGCN Q+QTC+YQVSYGDGS+T G+F TETLTFRRT+V RVALGCGHDNEGLFV
Sbjct: 192 CHRLDSPGCNTQKQTCMYQVSYGDGSFTFGDFSTETLTFRRTRVARVALGCGHDNEGLFV 251

Query: 251 GAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLL 310
           GAAGLLGLGRG LSFPSQTGR FN KFSYCLVDRSASSKPSS+VFG+SAVSRTARFTPL+
Sbjct: 252 GAAGLLGLGRGRLSFPSQTGRRFNHKFSYCLVDRSASSKPSSMVFGDSAVSRTARFTPLV 311

Query: 311 TNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTRLNRPAYIAL 370
           +NP+LDTFYYVELLGISVGG  V GI+   FKLD TGNGGVIID GTSVTRL RPAYIA 
Sbjct: 312 SNPKLDTFYYVELLGISVGGTRVPGITASLFKLDQTGNGGVIIDSGTSVTRLTRPAYIAF 371

Query: 371 RDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPASNYLIPVDGS 430
           RDAFRAGAS+LK A +FSLFDTC+DLSGKT VKVPTVVLHFR ADVSLPASNYLIPVD S
Sbjct: 372 RDAFRAGASNLKRAPQFSLFDTCFDLSGKTEVKVPTVVLHFRGADVSLPASNYLIPVDTS 431

Query: 431 GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           G FC AFAGT  GLSIIGNIQQQGFRVVYDLAGSRVGF+P GCA
Sbjct: 432 GNFCLAFAGTMGGLSIIGNIQQQGFRVVYDLAGSRVGFAPHGCA 469

BLAST of CmaCh14G021100 vs. TrEMBL
Match: V4TCM2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019938mg PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 3.6e-190
Identity = 345/473 (72.94%), Postives = 387/473 (81.82%), Query Frame = 1

Query: 11  IFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSS---EATESEPGLALH 70
           +  L +    + A   +QT V   LPT  +   PES   S+S SS    A ++E  L+L 
Sbjct: 9   LLLLFSFFFTAAASLQYQTFVLNSLPTQSTLSWPESVSVSESESSLPLPAPDAESSLSLR 68

Query: 71  LHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAAS-------RNVSRASGTGFSSSV 130
           LHH+DSLS +RTPE LF+LR+QRD LRV  L+  A ++       R+  RA+G GFSSSV
Sbjct: 69  LHHVDSLSFNRTPEHLFNLRIQRDVLRVKSLTAFAESAVRVPPRNRSRGRANG-GFSSSV 128

Query: 131 ISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGS 190
           ISGLAQGSGEYFTR+GVGTPPRYVY+VLDTGSD+VW+QCAPCK CYSQTDPVFDP KS S
Sbjct: 129 ISGLAQGSGEYFTRLGVGTPPRYVYMVLDTGSDVVWIQCAPCKKCYSQTDPVFDPAKSRS 188

Query: 191 FSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGC 250
           F+ V CR+PLC +L+S GCN+R TCLYQVSYGDGS T G+F TETLTFR T+V RVALGC
Sbjct: 189 FATVPCRSPLCRKLDSSGCNRRNTCLYQVSYGDGSITVGDFSTETLTFRGTRVARVALGC 248

Query: 251 GHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAVS 310
           GHDNEGLFV AAGLLGLGRG LSFP+QTGR FN+KFSYCLVDRS S+KPSS+VFG+SAVS
Sbjct: 249 GHDNEGLFVAAAGLLGLGRGRLSFPTQTGRRFNRKFSYCLVDRSTSAKPSSMVFGDSAVS 308

Query: 311 RTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTR 370
           RTARFTPLL NP+LDTFYYVEL+GISVGG  V GI+   FKLD  GNGGVIID GTSVTR
Sbjct: 309 RTARFTPLLANPKLDTFYYVELVGISVGGAHVRGITASLFKLDPAGNGGVIIDSGTSVTR 368

Query: 371 LNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPAS 430
           L RPAYIALRDAFRAGASSLK A +FSLFDTC+DLSGKT VKVPTVVLHFR ADVSLPA+
Sbjct: 369 LTRPAYIALRDAFRAGASSLKRAPDFSLFDTCFDLSGKTEVKVPTVVLHFRGADVSLPAT 428

Query: 431 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           NYLIPVD SG FCFAFAGT SGLSIIGNIQQQGFRVVYDLA SR+GF+PRGCA
Sbjct: 429 NYLIPVDSSGTFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAASRIGFAPRGCA 480

BLAST of CmaCh14G021100 vs. TrEMBL
Match: A0A067HET1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g040810mg PE=3 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 4.7e-190
Identity = 345/473 (72.94%), Postives = 387/473 (81.82%), Query Frame = 1

Query: 11  IFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSS---EATESEPGLALH 70
           +  L +    + A   +QT V   LPT  +   PES   S+S SS    A ++E  L+L 
Sbjct: 9   LLLLFSFFFTAAASLQYQTFVLNSLPTPSTLSWPESVSVSESESSLPLPAPDAESSLSLR 68

Query: 71  LHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAAS-------RNVSRASGTGFSSSV 130
           LHH+DSLS +RTPE LF+LR+QRD LRV  L+  A ++       R+  RA+G GFSSSV
Sbjct: 69  LHHVDSLSFNRTPEHLFNLRIQRDVLRVKSLTAFAESAVRVPPRNRSRGRANG-GFSSSV 128

Query: 131 ISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGS 190
           ISGLAQGSGEYFTR+GVGTPPRYVY+VLDTGSD+VW+QCAPCK CYSQTDPVFDP KS S
Sbjct: 129 ISGLAQGSGEYFTRLGVGTPPRYVYMVLDTGSDVVWIQCAPCKKCYSQTDPVFDPAKSRS 188

Query: 191 FSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGC 250
           F+ V CR+PLC +L+S GCN+R TCLYQVSYGDGS T G+F TETLTFR T+V RVALGC
Sbjct: 189 FATVPCRSPLCRKLDSSGCNRRNTCLYQVSYGDGSITVGDFSTETLTFRGTRVARVALGC 248

Query: 251 GHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAVS 310
           GHDNEGLFV AAGLLGLGRG LSFP+QTGR FN+KFSYCLVDRS S+KPSS+VFG+SAVS
Sbjct: 249 GHDNEGLFVAAAGLLGLGRGRLSFPTQTGRRFNRKFSYCLVDRSTSAKPSSMVFGDSAVS 308

Query: 311 RTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTR 370
           RTARFTPLL NP+LDTFYYVEL+GISVGG  V GI+   FKLD  GNGGVIID GTSVTR
Sbjct: 309 RTARFTPLLANPKLDTFYYVELVGISVGGAHVRGITASLFKLDPAGNGGVIIDSGTSVTR 368

Query: 371 LNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPAS 430
           L RPAYIALRDAFRAGASSLK A +FSLFDTC+DLSGKT VKVPTVVLHFR ADVSLPA+
Sbjct: 369 LTRPAYIALRDAFRAGASSLKRAPDFSLFDTCFDLSGKTEVKVPTVVLHFRGADVSLPAT 428

Query: 431 NYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           NYLIPVD SG FCFAFAGT SGLSIIGNIQQQGFRVVYDLA SR+GF+PRGCA
Sbjct: 429 NYLIPVDSSGTFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAASRIGFAPRGCA 480

BLAST of CmaCh14G021100 vs. TAIR10
Match: AT1G01300.1 (AT1G01300.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 627.5 bits (1617), Expect = 6.8e-180
Identity = 329/483 (68.12%), Postives = 377/483 (78.05%), Query Frame = 1

Query: 8   FPFIFFLLTLLPLSTAFSDFQTLVP-------------RPLPTSPSFLAPESTEGSDSFS 67
           F   FF L+L P  ++   FQTL P             +P   S S L  E   GSDS  
Sbjct: 10  FSLCFFFLSL-PSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDS-- 69

Query: 68  SEATESEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAA--SRNVSRAS 127
               ES   + L+L H+D+LS ++TP+ELF  RLQRD+ RV  ++ LAA    RNV+ A 
Sbjct: 70  ----ESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP 129

Query: 128 GTG-FSSSVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDP 187
             G FSSSV+SGL+QGSGEYFTR+GVGTP RYVY+VLDTGSDIVWLQCAPC+ CYSQ+DP
Sbjct: 130 RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDP 189

Query: 188 VFDPVKSGSFSKVLCRTPLCGRLESPGCN-QRQTCLYQVSYGDGSYTTGEFVTETLTFRR 247
           +FDP KS +++ + C +P C RL+S GCN +R+TCLYQVSYGDGS+T G+F TETLTFRR
Sbjct: 190 IFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRR 249

Query: 248 TKVERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPS 307
            +V+ VALGCGHDNEGLFVGAAGLLGLG+G LSFP QTG  FNQKFSYCLVDRSASSKPS
Sbjct: 250 NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPS 309

Query: 308 SVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGV 367
           SVVFGN+AVSR ARFTPLL+NP+LDTFYYV LLGISVGG  V G++   FKLD  GNGGV
Sbjct: 310 SVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGV 369

Query: 368 IIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHF 427
           IID GTSVTRL RPAYIA+RDAFR GA +LK A +FSLFDTC+DLS    VKVPTVVLHF
Sbjct: 370 IIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF 429

Query: 428 RNADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPR 474
           R ADVSLPA+NYLIPVD +G+FCFAFAGT  GLSIIGNIQQQGFRVVYDLA SRVGF+P 
Sbjct: 430 RGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPG 485

BLAST of CmaCh14G021100 vs. TAIR10
Match: AT3G61820.1 (AT3G61820.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 577.0 bits (1486), Expect = 1.1e-164
Identity = 308/481 (64.03%), Postives = 364/481 (75.68%), Query Frame = 1

Query: 6   SPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATESEPGLA 65
           S F  +FF       S+A S +QTLV   LP+S +   PES   +D   SE+T S   L+
Sbjct: 12  SVFAVLFFT------SSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTS---LS 71

Query: 66  LHLHHLDSLSL--SRTPEELFHLRLQRDALRVNKLSLLAAAS--RNVSRAS---GTGFSS 125
           +HL H+D+LS     +P +LF+LRLQRD+LRV  ++ LAA S  RN ++ +     GFS 
Sbjct: 72  VHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAGGFSG 131

Query: 126 SVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKS 185
           +VISGL+QGSGEYF R+GVGTP   VY+VLDTGSD+VWLQC+PCK CY+QTD +FDP KS
Sbjct: 132 AVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKS 191

Query: 186 GSFSKVLCRTPLCGRLE-SPGCNQRQ--TCLYQVSYGDGSYTTGEFVTETLTFRRTKVER 245
            +F+ V C + LC RL+ S  C  R+  TCLYQVSYGDGS+T G+F TETLTF   +V+ 
Sbjct: 192 KTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDH 251

Query: 246 VALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDR----SASSKPSS 305
           V LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQT   +N KFSYCLVDR    S+S  PS+
Sbjct: 252 VPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPST 311

Query: 306 VVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVI 365
           +VFGN+AV +T+ FTPLLTNP+LDTFYY++LLGISVGG  V G+S   FKLD+TGNGGVI
Sbjct: 312 IVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVI 371

Query: 366 IDCGTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFR 425
           ID GTSVTRL +PAY+ALRDAFR GA+ LK A  +SLFDTC+DLSG TTVKVPTVV HF 
Sbjct: 372 IDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFG 431

Query: 426 NADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRG 473
             +VSLPASNYLIPV+  GRFCFAFAGT   LSIIGNIQQQGFRV YDL GSRVGF  R 
Sbjct: 432 GGEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRA 483

BLAST of CmaCh14G021100 vs. TAIR10
Match: AT1G25510.1 (AT1G25510.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 400.6 bits (1028), Expect = 1.3e-111
Identity = 238/489 (48.67%), Postives = 299/489 (61.15%), Query Frame = 1

Query: 8   FPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFL----APESTEGSDSFSSEATESEP- 67
           + F FF+  L   S+ FS    ++P    T+ S L    +   T+ + SF     E +  
Sbjct: 5   YSFFFFIFFLTSHSSVFS---RILPETSTTTTSILNVADSIHRTKYTSSFRLNQQEEQTH 64

Query: 68  ----GLALHLHHLDSLSLSRTPE--ELFHLRLQRDALRVNKL-SLLAAASRNVSRASGTG 127
                 +L LH   S+  +   +   L   RL RD  RV  L + L  A  N+S+A    
Sbjct: 65  SASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKP 124

Query: 128 FSSS-----------VISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKN 187
            S+            +ISG  QGSGEYFTR+G+G P R VY+VLDTGSD+ WLQC PC +
Sbjct: 125 ISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCAD 184

Query: 188 CYSQTDPVFDPVKSGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTE 247
           CY QT+P+F+P  S S+  + C TP C  LE   C +  TCLY+VSYGDGSYT G+F TE
Sbjct: 185 CYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSEC-RNATCLYEVSYGDGSYTVGDFATE 244

Query: 248 TLTFRRTKVERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRS 307
           TLT   T V+ VA+GCGH NEGLFVGAAGLLGLG G L+ PSQ        FSYCLVDR 
Sbjct: 245 TLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLN---TTSFSYCLVDRD 304

Query: 308 ASSKPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDS 367
           + S  S+V FG S +S  A   PLL N +LDTFYY+ L GISVGG  +  I    F++D 
Sbjct: 305 SDS-ASTVDFGTS-LSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQ-IPQSSFEMDE 364

Query: 368 TGNGGVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVP 427
           +G+GG+IID GT+VTRL    Y +LRD+F  G   L+ AA  ++FDTCY+LS KTTV+VP
Sbjct: 365 SGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVP 424

Query: 428 TVVLHFRNAD-VSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGS 473
           TV  HF     ++LPA NY+IPVD  G FC AFA T S L+IIGN+QQQG RV +DLA S
Sbjct: 425 TVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANS 483

BLAST of CmaCh14G021100 vs. TAIR10
Match: AT3G20015.1 (AT3G20015.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 394.4 bits (1012), Expect = 9.7e-110
Identity = 222/473 (46.93%), Postives = 293/473 (61.95%), Query Frame = 1

Query: 10  FIFFLLTLLPLSTA----FSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATESEPGLA 69
           F FFL   L LS++    F DFQ +     P + +   P+    +  FS E++       
Sbjct: 6   FFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFN--NTHFSDESSSKYTLRL 65

Query: 70  LHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNV-----SRASGTGFSSSV 129
           LH     S++  R      H R++RD  RV+  ++L   S  V     SR     F S +
Sbjct: 66  LHRDRFPSVTY-RNHHHRLHARMRRDTDRVS--AILRRISGKVIPSSDSRYEVNDFGSDI 125

Query: 130 ISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGS 189
           +SG+ QGSGEYF RIGVG+PPR  Y+V+D+GSD+VW+QC PCK CY Q+DPVFDP KSGS
Sbjct: 126 VSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGS 185

Query: 190 FSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGC 249
           ++ V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA+GC
Sbjct: 186 YTGVSCGSSVCDRIENSGCHSGG-CRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGC 245

Query: 250 GHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAVS 309
           GH N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  A+ 
Sbjct: 246 GHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREALP 305

Query: 310 RTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTR 369
             A + PL+ NPR  +FYYV L G+ VGG  +  +    F L  TG+GGV++D GT+VTR
Sbjct: 306 VGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTAVTR 365

Query: 370 LNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADV-SLPA 429
           L   AY+A RD F++  ++L  A+  S+FDTCYDLSG  +V+VPTV  +F    V +LPA
Sbjct: 366 LPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPA 425

Query: 430 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 473
            N+L+PVD SG +CFAFA + +GLSIIGNIQQ+G +V +D A   VGF P  C
Sbjct: 426 RNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of CmaCh14G021100 vs. TAIR10
Match: AT3G18490.1 (AT3G18490.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 388.3 bits (996), Expect = 6.9e-108
Identity = 225/475 (47.37%), Postives = 291/475 (61.26%), Query Frame = 1

Query: 17  LLPLSTAFSDFQTLVPR-PLPTSPSFLAPESTEGSDSFSSEATESEPGLALHLHHLDSLS 76
           +L + ++    QT++   P  +S +   PES      F+S +      L+L LH  D+  
Sbjct: 37  VLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSDPVFFNSSSP-----LSLELHSRDTFV 96

Query: 77  LSRTPE--ELFHLRLQRDALRVNKL-------------SLLAAASRNVSRASGTGFSSSV 136
            S+  +   L   RL+RD+ RV  +             S L       +R      ++ V
Sbjct: 97  ASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPV 156

Query: 137 ISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGS 196
           +SG +QGSGEYF+RIGVGTP + +YLVLDTGSD+ W+QC PC +CY Q+DPVF+P  S +
Sbjct: 157 VSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSST 216

Query: 197 FSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRT-KVERVALG 256
           +  + C  P C  LE+  C   + CLYQVSYGDGS+T GE  T+T+TF  + K+  VALG
Sbjct: 217 YKSLTCSAPQCSLLETSACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALG 276

Query: 257 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAV 316
           CGHDNEGLF GAAGLLGLG G LS  +Q        FSYCLVDR  S K SS+ F +  +
Sbjct: 277 CGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQL 336

Query: 317 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 376
                  PLL N ++DTFYYV L G SVGG  V  +    F +D++G+GGVI+DCGT+VT
Sbjct: 337 GGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVV-LPDAIFDVDASGSGGVILDCGTAVT 396

Query: 377 RLNRPAYIALRDAF-RAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNA-DVSL 436
           RL   AY +LRDAF +   +  K ++  SLFDTCYD S  +TVKVPTV  HF     + L
Sbjct: 397 RLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDL 456

Query: 437 PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 473
           PA NYLIPVD SG FCFAFA T+S LSIIGN+QQQG R+ YDL+ + +G S   C
Sbjct: 457 PAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CmaCh14G021100 vs. NCBI nr
Match: gi|659074959|ref|XP_008437888.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo])

HSP 1 Score: 825.9 bits (2132), Expect = 3.7e-236
Identity = 422/475 (88.84%), Postives = 438/475 (92.21%), Query Frame = 1

Query: 1   MVAKTSPFPFIFFLL-TLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATE 60
           M A T   PFIFFLL  +L LSTAFSDFQTL+ R LP+SPSFL  +S   +   SSEATE
Sbjct: 3   MEANTISLPFIFFLLLAILSLSTAFSDFQTLILRSLPSSPSFLPSDS---NSFLSSEATE 62

Query: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGT-GFSS 120
           +E GL LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A SRN+SR SGT GFSS
Sbjct: 63  TELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSS 122

Query: 121 SVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKS 180
           SVISGLAQGSGEYFTRIGVGTPP+YVY+VLDTGSDIVWLQCAPCKNCYSQTDPVF+PVKS
Sbjct: 123 SVISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKS 182

Query: 181 GSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVAL 240
           GSF+KVLCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VAL
Sbjct: 183 GSFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVAL 242

Query: 241 GCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSA 300
           GCGHDNEGLFVGAAGLLGLGRGGLSFPSQ GR FNQKFSYCLVDRSASSKPSSVVFGNSA
Sbjct: 243 GCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSA 302

Query: 301 VSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSV 360
           VSRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTSV
Sbjct: 303 VSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSV 362

Query: 361 TRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLP 420
           TRLN+PAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLP
Sbjct: 363 TRLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLP 422

Query: 421 ASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           ASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 423 ASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474

BLAST of CmaCh14G021100 vs. NCBI nr
Match: gi|449432044|ref|XP_004133810.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis sativus])

HSP 1 Score: 820.8 bits (2119), Expect = 1.2e-234
Identity = 417/474 (87.97%), Postives = 435/474 (91.77%), Query Frame = 1

Query: 1   MVAKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATES 60
           M   T   PFIFFLLT+L L+TAFSDFQTL    LP+SPSFL  +S   +   SSEAT+S
Sbjct: 1   MEPNTISLPFIFFLLTVLSLATAFSDFQTLPLTSLPSSPSFLPSDS---NSFLSSEATQS 60

Query: 61  EPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGT-GFSSS 120
           E GL LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A SRN+S+  GT GFSSS
Sbjct: 61  ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSKPGGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180
           VISGLAQGSGEYFTRIGVGTPP+YVY+VLDTGSDIVWLQCAPCKNCYSQTDPVF+PVKSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 180

Query: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SF+KVLCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VALG
Sbjct: 181 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GR FNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGI+  HFKLD TGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGITASHFKLDRTGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420
           RLN+PAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLPA
Sbjct: 361 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 471

BLAST of CmaCh14G021100 vs. NCBI nr
Match: gi|700201288|gb|KGN56421.1| (Aspartic proteinase nepenthesin-1 [Cucumis sativus])

HSP 1 Score: 820.8 bits (2119), Expect = 1.2e-234
Identity = 417/474 (87.97%), Postives = 435/474 (91.77%), Query Frame = 1

Query: 1   MVAKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATES 60
           M   T   PFIFFLLT+L L+TAFSDFQTL    LP+SPSFL  +S   +   SSEAT+S
Sbjct: 42  MEPNTISLPFIFFLLTVLSLATAFSDFQTLPLTSLPSSPSFLPSDS---NSFLSSEATQS 101

Query: 61  EPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGT-GFSSS 120
           E GL LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A SRN+S+  GT GFSSS
Sbjct: 102 ELGLELHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSKPGGTTGFSSS 161

Query: 121 VISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180
           VISGLAQGSGEYFTRIGVGTPP+YVY+VLDTGSDIVWLQCAPCKNCYSQTDPVF+PVKSG
Sbjct: 162 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 221

Query: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SF+KVLCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VALG
Sbjct: 222 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALG 281

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQ GR FNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 282 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 341

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGI+  HFKLD TGNGGVIIDCGTSVT
Sbjct: 342 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGITASHFKLDRTGNGGVIIDCGTSVT 401

Query: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420
           RLN+PAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLPA
Sbjct: 402 RLNKPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 461

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 462 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 512

BLAST of CmaCh14G021100 vs. NCBI nr
Match: gi|1009142522|ref|XP_015888767.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Ziziphus jujuba])

HSP 1 Score: 678.7 bits (1750), Expect = 7.3e-192
Identity = 350/469 (74.63%), Postives = 390/469 (83.16%), Query Frame = 1

Query: 12  FFLLTLLPLSTAFSD---FQTLVPRPLPTSP--SFLAPESTEGSDSFSSEA-TESEP-GL 71
           F    +L LS AF+D   +QTL  +PLP +P  S+   ES  G+D+   EA T S P  L
Sbjct: 12  FSFAIILTLSAAFTDPLEYQTLTLKPLPNAPTLSWTDSESESGTDATELEAETSSTPTSL 71

Query: 72  ALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGTGFSSSVISGL 131
           ++ L H+D+LS ++TPE+LF LR+QRDALRV  L  L A  RN +R+ G+GFSSSVISGL
Sbjct: 72  SVQLQHIDALSFNKTPEDLFGLRIQRDALRVKTLDSLLAV-RNQTRSRGSGFSSSVISGL 131

Query: 132 AQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGSFSKV 191
           AQGSGEYFTR+GVGTPPRYVY+VLDTGSDIVWLQCAPCK CY+QTDPVFDP KS SF  +
Sbjct: 132 AQGSGEYFTRLGVGTPPRYVYMVLDTGSDIVWLQCAPCKKCYTQTDPVFDPRKSRSFVGI 191

Query: 192 LCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCGHDN 251
            C +PLC +L+SPGCNQR+ CLYQVSYGDGS+T GEF TETLTFRR++V RVALGCGHDN
Sbjct: 192 SCSSPLCRKLDSPGCNQRKMCLYQVSYGDGSFTLGEFSTETLTFRRSRVARVALGCGHDN 251

Query: 252 EGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR 311
           EGLFVGAAGLLGLGRG LSFP+QTG  FNQKFSYCLVDRSASSKPSS+VFGN+A+SR AR
Sbjct: 252 EGLFVGAAGLLGLGRGKLSFPTQTGLRFNQKFSYCLVDRSASSKPSSIVFGNAAISRAAR 311

Query: 312 FTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTRLNRP 371
           FTPLL NP+LDTFYYVEL+GISVGG  V GI+   FKLD+ GNGGVIID GTSVTRL R 
Sbjct: 312 FTPLLRNPKLDTFYYVELIGISVGGTRVPGITASLFKLDTAGNGGVIIDSGTSVTRLTRT 371

Query: 372 AYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPASNYLI 431
           AY ALRDAFR GASSLK A EFSLFDTC+DLSG   VKVPTVVLHFR ADVSLPA+NYLI
Sbjct: 372 AYTALRDAFRVGASSLKRAPEFSLFDTCFDLSGLREVKVPTVVLHFRGADVSLPATNYLI 431

Query: 432 PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           PVD  G FCFAFAGT SGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 432 PVDSGGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 479

BLAST of CmaCh14G021100 vs. NCBI nr
Match: gi|703096146|ref|XP_010095748.1| (Aspartic proteinase nepenthesin-1 [Morus notabilis])

HSP 1 Score: 674.5 bits (1739), Expect = 1.4e-190
Identity = 350/475 (73.68%), Postives = 390/475 (82.11%), Query Frame = 1

Query: 3   AKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATESEP 62
           A T P  +   LLT LP+       QTL     P S S L     E S++ ++E TE+  
Sbjct: 26  ASTPPLEYETLLLTSLPIPQ-----QTL---SWPDSESELTGSDLE-SETAAAEETETSL 85

Query: 63  GLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLL--AAASRNVSRASGT--GFSS 122
            ++  LHH+D+LS  ++PE+LF LRLQRDALRV  L  +  AAASRNVSR  G   GFSS
Sbjct: 86  SISAQLHHIDALSADKSPEQLFDLRLQRDALRVKNLVEVTAAAASRNVSRTRGAAPGFSS 145

Query: 123 SVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKS 182
           SVISGLAQGSGEYFTR+GVGTPPRYVY+VLDTGSD+VWLQCAPC+ CY+Q DPVFDP KS
Sbjct: 146 SVISGLAQGSGEYFTRLGVGTPPRYVYMVLDTGSDVVWLQCAPCRKCYTQADPVFDPSKS 205

Query: 183 GSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVAL 242
            SF+++ C +PLC +L+SPGCNQR+ CLYQVSYGDGS+TTGEF TETLTFRRT++ RVAL
Sbjct: 206 RSFARISCGSPLCRKLDSPGCNQRKMCLYQVSYGDGSFTTGEFSTETLTFRRTRIGRVAL 265

Query: 243 GCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSA 302
           GCGHDNEGLFVGAAGLLGLGRG LSFP QTG  FN+KFSYCL DRSASSKPSS+VFG+SA
Sbjct: 266 GCGHDNEGLFVGAAGLLGLGRGRLSFPFQTGLRFNRKFSYCLADRSASSKPSSMVFGDSA 325

Query: 303 VSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSV 362
           VSRTARFTPLLTNP+LDTFYY+ELL ISVGG  V GIS   FKLD  GNGGVIID GTSV
Sbjct: 326 VSRTARFTPLLTNPKLDTFYYLELLAISVGGSRVRGISASLFKLDQAGNGGVIIDSGTSV 385

Query: 363 TRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLP 422
           TRL RPAY+ALRDAFRAG+ +LK A EFSLFDTCYDLSGKT VKVPTVVLHFR ADVS P
Sbjct: 386 TRLTRPAYVALRDAFRAGSVNLKRAPEFSLFDTCYDLSGKTEVKVPTVVLHFRGADVSFP 445

Query: 423 ASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474
           A+NYLIPVD SG FCFAFAGT SGLSIIGNIQQQGFRVVYDLAGSRVGF+PRGCA
Sbjct: 446 ATNYLIPVDSSGTFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRVGFAPRGCA 491

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
APF2_ARATH1.2e-17868.12Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1[more]
ASPG2_ARATH1.7e-10846.93Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 ... [more]
ASPG1_ARATH1.2e-10647.37Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 ... [more]
ASPA_ARATH3.6e-7440.64Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana GN=At5g10770 ... [more]
AED1_ARATH3.1e-7340.71Aspartyl protease AED1 OS=Arabidopsis thaliana GN=AED1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L8K0_CUCSA8.3e-23587.97Aspartic proteinase nepenthesin-1 OS=Cucumis sativus GN=Csa_3G119540 PE=3 SV=1[more]
W9R017_9ROSA9.6e-19173.68Aspartic proteinase nepenthesin-1 OS=Morus notabilis GN=L484_017553 PE=3 SV=1[more]
B9SBG8_RICCO1.2e-19074.35Aspartic proteinase nepenthesin-1, putative OS=Ricinus communis GN=RCOM_0717990 ... [more]
V4TCM2_9ROSI3.6e-19072.94Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019938mg PE=3 SV=1[more]
A0A067HET1_CITSI4.7e-19072.94Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g040810mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G01300.16.8e-18068.12 Eukaryotic aspartyl protease family protein[more]
AT3G61820.11.1e-16464.03 Eukaryotic aspartyl protease family protein[more]
AT1G25510.11.3e-11148.67 Eukaryotic aspartyl protease family protein[more]
AT3G20015.19.7e-11046.93 Eukaryotic aspartyl protease family protein[more]
AT3G18490.16.9e-10847.37 Eukaryotic aspartyl protease family protein[more]
Match NameE-valueIdentityDescription
gi|659074959|ref|XP_008437888.1|3.7e-23688.84PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo][more]
gi|449432044|ref|XP_004133810.1|1.2e-23487.97PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis sativus][more]
gi|700201288|gb|KGN56421.1|1.2e-23487.97Aspartic proteinase nepenthesin-1 [Cucumis sativus][more]
gi|1009142522|ref|XP_015888767.1|7.3e-19274.63PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Ziziphus jujuba][more]
gi|703096146|ref|XP_010095748.1|1.4e-19073.68Aspartic proteinase nepenthesin-1 [Morus notabilis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001461Aspartic_peptidase_A1
IPR001969Aspartic_peptidase_AS
IPR021109Peptidase_aspartic_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0009664 plant-type cell wall organization
biological_process GO:0006508 proteolysis
biological_process GO:0080167 response to karrikin
cellular_component GO:0016020 membrane
cellular_component GO:0009505 plant-type cell wall
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0004190 aspartic-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G021100.1CmaCh14G021100.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 444..459
score: 6.0E-6coord: 137..157
score: 6.0E-6coord: 350..361
score: 6.
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 54..472
score: 4.1E-244coord: 4..26
score: 4.1E
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 146..157
scor
IPR021109Aspartic peptidase domainGENE3DG3DSA:2.40.70.10coord: 128..296
score: 3.5E-40coord: 298..473
score: 2.3
IPR021109Aspartic peptidase domainunknownSSF50630Acid proteasescoord: 124..472
score: 1.73E
NoneNo IPR availablePANTHERPTHR13683:SF308ASPARTYL PROTEASE-RELATEDcoord: 4..26
score: 4.1E-244coord: 54..472
score: 4.1E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh14G021100CmaCh00G002950Cucurbita maxima (Rimu)cmacmaB008