CmaCh13G004080 (gene) Cucurbita maxima (Rimu)

NameCmaCh13G004080
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPhloem protein 2
LocationCma_Chr13 : 4645214 .. 4646593 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAGATTATTATAGACGGTAAAACAGTTCGGTATCAAATAGACATAGAGATAGACACGCGTATGAAAATAAGAAATAAGCATGGCTTAGGTTGAAAATAGTGCAGCAAAGAAGGGGTTATATATATCCCTTCTTCCCTCTCACATTAACTCATATCTCACTTCTGTTCATAAAGAGAAGGCACTGCAATGGACAACAAAGAGAAGGAAGCCAGAGAGAAATTAGGAGGAGAAGTGAAGCTCGGTCATTGCTTGGATGTTATTTTGAAGAATGCTGACGTAGCACTGCACTATCCCTCCTTCCTTAAGCTTTATGACCAACTTGTTGCTGGGATCCTCTTGAACAAGGGAGCTATAGTAAGTGCAACCATATATACTTCAACTCATTTTACTCAGCTTTGTATATCATAACCATATTAAATCAGAATATTGGCTTTCTTTACTTTGAATGCAGAAGTACATCTTTGATAAGAAGTCAAACAGCAACTGGTACTTTATATTTGCAAGAGCTCTCTCAATAGCTTGGATTGAAGATAAGAGATACTGGAAATGGGGATCCTGGTATAATTTTTTAACTAATTTCTCAAGGGGAAAAAATGATAAGAACTTGATTTCCTGATCTCTCTCACTCGGGTGTCTAAACACTTGCAGTGGCGATAGCAACGTTGCAGAGCTTATTGAAGTATCTTGGCTGGACATTCGTGGAAAGATCAACGAGTCTATGCTCTCACAAAATGTTGTGTATGAGGTAGCACTTCAGGTACAGCTGAATAGTAGAGCCTCCGGGTGGAATGCTCCACTGAACATCGAGTTGAAGAAGCCAGATGGGAGCAAGATAGCGCGCCAGGAATGCCTGTTGGGGAAGCCACAAAACCAGTGGTTTGAGATTGTTGTTGAGTTCAAGGTAGGCAACCATGGCTGTGGAAGTAGCGGCGAGATCGAGTTTGCCTTTTTTGAACATGGAGGGCATTGGAAGAGGGGGCTGCTCGTGAAAGGCGTTCGGATTGGAGCAAAGGGATGTGGTTGCGCATGATCGAAATCCTCTCTCTCGAACTCAGACTACACTTATTTTGATTTTGAGAGGCCAGAGTTTGTGTTATGATCCAATATGAAAAGAATGTACTAGCTTGCAAACATAAATAACAGCACCTTTTGCTTACCGGCAATAAGGTCAAGTTTTAAATACATTTTGTTTTAGATACAATAAAATATACGTAATACTACTTTTTTTTTGGTTTACCAATCCGGGTAAGTATAAACACAGCAAACAATTACGTGAAACTCGTATTGGTTCTCATGCTTCACCGACTTTTAGCTTACTAAACGGTAAACATGGTAACAGAGAATCATTTTTTTAGAGGTTAGTTGATAGTTTGTATCTT

mRNA sequence

ATAGATTATTATAGACGGTAAAACAGTTCGGTATCAAATAGACATAGAGATAGACACGCGTATGAAAATAAGAAATAAGCATGGCTTAGGTTGAAAATAGTGCAGCAAAGAAGGGGTTATATATATCCCTTCTTCCCTCTCACATTAACTCATATCTCACTTCTGTTCATAAAGAGAAGGCACTGCAATGGACAACAAAGAGAAGGAAGCCAGAGAGAAATTAGGAGGAGAAGTGAAGCTCGGTCATTGCTTGGATGTTATTTTGAAGAATGCTGACGTAGCACTGCACTATCCCTCCTTCCTTAAGCTTTATGACCAACTTGTTGCTGGGATCCTCTTGAACAAGGGAGCTATAAAGTACATCTTTGATAAGAAGTCAAACAGCAACTGGTACTTTATATTTGCAAGAGCTCTCTCAATAGCTTGGATTGAAGATAAGAGATACTGGAAATGGGGATCCTGTGGCGATAGCAACGTTGCAGAGCTTATTGAAGTATCTTGGCTGGACATTCGTGGAAAGATCAACGAGTCTATGCTCTCACAAAATGTTGTGTATGAGGTAGCACTTCAGGTACAGCTGAATAGTAGAGCCTCCGGGTGGAATGCTCCACTGAACATCGAGTTGAAGAAGCCAGATGGGAGCAAGATAGCGCGCCAGGAATGCCTGTTGGGGAAGCCACAAAACCAGTGGTTTGAGATTGTTGTTGAGTTCAAGGTAGGCAACCATGGCTGTGGAAGTAGCGGCGAGATCGAGTTTGCCTTTTTTGAACATGGAGGGCATTGGAAGAGGGGGCTGCTCGTGAAAGGCGTTCGGATTGGAGCAAAGGGATGTGGTTGCGCATGATCGAAATCCTCTCTCTCGAACTCAGACTACACTTATTTTGATTTTGAGAGGCCAGAGTTTGTGTTATGATCCAATATGAAAAGAATGTACTAGCTTGCAAACATAAATAACAGCACCTTTTGCTTACCGGCAATAAGGTCAAGTTTTAAATACATTTTGTTTTAGATACAATAAAATATACGTAATACTACTTTTTTTTTGGTTTACCAATCCGGGTAAGTATAAACACAGCAAACAATTACGTGAAACTCGTATTGGTTCTCATGCTTCACCGACTTTTAGCTTACTAAACGGTAAACATGGTAACAGAGAATCATTTTTTTAGAGGTTAGTTGATAGTTTGTATCTT

Coding sequence (CDS)

ATGGACAACAAAGAGAAGGAAGCCAGAGAGAAATTAGGAGGAGAAGTGAAGCTCGGTCATTGCTTGGATGTTATTTTGAAGAATGCTGACGTAGCACTGCACTATCCCTCCTTCCTTAAGCTTTATGACCAACTTGTTGCTGGGATCCTCTTGAACAAGGGAGCTATAAAGTACATCTTTGATAAGAAGTCAAACAGCAACTGGTACTTTATATTTGCAAGAGCTCTCTCAATAGCTTGGATTGAAGATAAGAGATACTGGAAATGGGGATCCTGTGGCGATAGCAACGTTGCAGAGCTTATTGAAGTATCTTGGCTGGACATTCGTGGAAAGATCAACGAGTCTATGCTCTCACAAAATGTTGTGTATGAGGTAGCACTTCAGGTACAGCTGAATAGTAGAGCCTCCGGGTGGAATGCTCCACTGAACATCGAGTTGAAGAAGCCAGATGGGAGCAAGATAGCGCGCCAGGAATGCCTGTTGGGGAAGCCACAAAACCAGTGGTTTGAGATTGTTGTTGAGTTCAAGGTAGGCAACCATGGCTGTGGAAGTAGCGGCGAGATCGAGTTTGCCTTTTTTGAACATGGAGGGCATTGGAAGAGGGGGCTGCTCGTGAAAGGCGTTCGGATTGGAGCAAAGGGATGTGGTTGCGCATGA

Protein sequence

MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIFDKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQNVVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNHGCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA
BLAST of CmaCh13G004080 vs. Swiss-Prot
Match: LEC_LUFAC (Lectin OS=Luffa acutangula PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 4.0e-44
Identity = 100/211 (47.39%), Postives = 132/211 (62.56%), Query Frame = 1

Query: 10  EKLGGEVKLGHCLDVILKNADVALH-YPSFLKLYDQLVAGILLNKGAIKYIFDKKSNSNW 69
           E +GGEVK+GH L+ ILK  DV ++  PSF+KLYDQ+ AGI LN    +Y FDK + SN 
Sbjct: 2   ELVGGEVKVGHNLEAILKGLDVDVYSVPSFIKLYDQVTAGIFLNNRTKRYWFDKNAESNC 61

Query: 70  YFIFARALSIAWIEDKRYWKWGSCGDSN----VAELIEVSWLDIRGKINESMLSQNVVYE 129
           + ++AR L I W +DKRYW+W    +      VAELI+V WL+I G I  S+LS  + YE
Sbjct: 62  FMLYARDLLITWSQDKRYWRWNPFQEHGNTLEVAELIDVCWLNIVGNIETSVLSPGISYE 121

Query: 130 VALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVV-EFKVGNHGCG 189
            A +V L + ASGW  P++++LK PDGS+   Q  L  KP+  WF I V  FK+     G
Sbjct: 122 AAFEVMLTNSASGWRIPVDVKLKMPDGSEQESQVNLQDKPRGVWFFISVGHFKI---SVG 181

Query: 190 SS-GEIEFAFFEHGGHWKRGLLVKGVRIGAK 214
            + G IEF+  +H    KRGLLVKG+ I  K
Sbjct: 182 ETIGNIEFSIVQH-QEAKRGLLVKGLVIQPK 208

BLAST of CmaCh13G004080 vs. Swiss-Prot
Match: P2A01_ARATH (Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana GN=PP2A1 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 7.6e-27
Identity = 76/207 (36.71%), Postives = 116/207 (56.04%), Query Frame = 1

Query: 16  VKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLN-KGAIKYIFDKKSNSNWYFIFAR 75
           VK  H  + IL++AD  +   S + L +QL +G+ L  K  IKY  D++ NSN + +FA+
Sbjct: 43  VKSPHNCEAILRDADPPISLSS-VNLSEQLRSGVFLKPKKQIKYWVDER-NSNCFMLFAK 102

Query: 76  ALSIAWIEDKRYWKWGSCGDS-----NVAELIEVSWLDIRGKINESMLSQNVVYEVALQV 135
            LSI W +D  YW W +  +S         L  V WLDI GK +   L+  +VYEV  +V
Sbjct: 103 NLSITWSDDVNYWTWFTEKESPNENVEAVGLKNVCWLDITGKFDTRNLTPGIVYEVVFKV 162

Query: 136 QLNSRASGWNAPLNIELKKPDGSKIARQE--CLLGKPQNQWFEIVVEFKVGNHGCGSSGE 195
           +L   A GW+ P+N++L  P+G +  +++   L   P+ +W ++ V   V      ++GE
Sbjct: 163 KLEDPAYGWDTPVNLKLVLPNGKEKPQEKKVSLRELPRYKWVDVRVGEFVPEK--SAAGE 222

Query: 196 IEFAFFEH-GGHWKRGLLVKGVRIGAK 214
           I F+ +EH  G WK+GL +KGV I  K
Sbjct: 223 ITFSMYEHAAGVWKKGLSLKGVAIRPK 245

BLAST of CmaCh13G004080 vs. Swiss-Prot
Match: P2A04_ARATH (Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana GN=PP2A4 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.8e-18
Identity = 60/156 (38.46%), Postives = 77/156 (49.36%), Query Frame = 1

Query: 69  YFIFARALSIAWIEDKRYWKW-------GSCGDSNVAELIEVSWLDIRGKINESMLSQNV 128
           + I+AR LSIAW +   YW W        S    + A L  V WLD+ GK +   L+   
Sbjct: 12  HMIYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLET 71

Query: 129 VYEVALQVQLNSRASGWNAPLNIELKKPDGSK--IARQECLLGKPQNQWFEIVV-EFKVG 188
            YEV   V+L   ASGWN P+N++L  PDG K    R  CL      +W +I   EF   
Sbjct: 72  TYEVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEFVTS 131

Query: 189 NHGCGSSGEIEFAFFE-HGGHWKRGLLVKGVRIGAK 214
                ++GEI F+ +E     WKRGL VK V I  K
Sbjct: 132 P---DNAGEISFSMYETKSCCWKRGLFVKCVEIRPK 164

BLAST of CmaCh13G004080 vs. Swiss-Prot
Match: P2B11_ARATH (F-box protein PP2-B11 OS=Arabidopsis thaliana GN=PP2B11 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.3e-14
Identity = 60/196 (30.61%), Postives = 93/196 (47.45%), Query Frame = 1

Query: 36  PSFLKLYDQLVAG-ILLNKGAIKYIFDKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGD 95
           P+  +L+  LV   +L+N   + +  ++KS +  Y + ARAL+I W  ++RYW W S  +
Sbjct: 60  PTRKQLFFSLVDNPLLINGTLLSFSLERKSGNKCYMMAARALNIVWGHEQRYWHWISLPN 119

Query: 96  SN---VAELIEVSWLDIRGKINESMLSQNVVYEVALQVQLNSRASGWNAPLNIELKKPDG 155
           +    VAELI V WL+I GKIN ++LS + +Y      + N    G+  P+   L   D 
Sbjct: 120 TRFGEVAELIMVWWLEITGKINITLLSDDTLYAAYFVFKWNHSPYGFRQPVETSLVLADT 179

Query: 156 SKIAR--QECLLGKPQNQ--------------WFEIVVEFKVGNHGCGSSGEIEFAFFE- 211
                  Q  ++   Q+               W+E  VE        G  GEIE +  E 
Sbjct: 180 ESTDNVVQPSMISLMQDSGGEEGQSPVLRRDGWYE--VELGQFFKRRGDLGEIEMSLKET 239

BLAST of CmaCh13G004080 vs. Swiss-Prot
Match: P2A02_ARATH (Protein PHLOEM PROTEIN 2-LIKE A2 OS=Arabidopsis thaliana GN=PP2A2 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.2e-13
Identity = 54/170 (31.76%), Postives = 84/170 (49.41%), Query Frame = 1

Query: 46  VAGILLNKGAIKYIFDKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSW 105
           V  ++L K      + KK    ++  F  +L+ A++     +   S   + VA++  V+W
Sbjct: 31  VGSLILTKHQGYEFYCKKVTFVFFCFFKISLNSAYL-----YTLYSDVRTEVAKMERVAW 90

Query: 106 LDIRGKINESMLSQNVVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQ 165
           L++ GK     L+ N +YEV   V+L   A GW+  +N +L  P G    R+E +    +
Sbjct: 91  LEVVGKFETEKLTPNSLYEVVFVVKLIDSAKGWDFRVNFKLVLPTGETKERRENVNLLER 150

Query: 166 NQWFEIVV-EFKVGNHGCGSSGEIEFAFFE-HGGHWKRGLLVKGVRIGAK 214
           N+W EI   EF +       SG+IEF+  E     WK GL+VKGV I  K
Sbjct: 151 NKWVEIPAGEFMISPEHL--SGKIEFSMLEVKSDQWKSGLIVKGVAIRPK 193

BLAST of CmaCh13G004080 vs. TrEMBL
Match: Q42383_CUCMA (Dimeric phloem specific lectin PP2 OS=Cucurbita maxima GN=PP2 PE=2 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 1.4e-125
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA
Sbjct: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 218

BLAST of CmaCh13G004080 vs. TrEMBL
Match: Q8LK66_9ROSI (Phloem protein 2 OS=Cucurbita digitata GN=PP2 PE=2 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 2.3e-123
Identity = 215/218 (98.62%), Postives = 216/218 (99.08%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           VVYEVALQVQLNSRASGWNAPLNIELKKPDGS+IARQECLLGKPQNQWFEIVVEFKVGNH
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSRIARQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEFAFFEHGG W RGLLVKGVRIGAKGCGCA
Sbjct: 181 GCGSSGEIEFAFFEHGGAWARGLLVKGVRIGAKGCGCA 218

BLAST of CmaCh13G004080 vs. TrEMBL
Match: Q7DLM2_CUCMA (PP2 lectin polypeptide OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.4e-120
Identity = 209/218 (95.87%), Postives = 214/218 (98.17%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKK NS+WYFIFARALSIAWIEDKRYWKWGSCG+S VAELIEVSWL+IRGKINESMLS N
Sbjct: 61  DKKLNSHWYFIFARALSIAWIEDKRYWKWGSCGNSEVAELIEVSWLNIRGKINESMLSPN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKI RQECLLGKPQNQWFEIVVEFKVGNH
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIVRQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEF+FFEHGGHWKRGLLVKGVRIGAKGCGC+
Sbjct: 181 GCGSSGEIEFSFFEHGGHWKRGLLVKGVRIGAKGCGCS 218

BLAST of CmaCh13G004080 vs. TrEMBL
Match: Q9LLT3_CUCMO (Phloem protein 2 OS=Cucurbita moschata GN=pp2 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 1.2e-116
Identity = 202/218 (92.66%), Postives = 211/218 (96.79%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           MDNKE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC    +AELIEVSWLDIRGKINESMLS N
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC--IKIAELIEVSWLDIRGKINESMLSPN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           +VYEVALQVQLN RASGW+APLNIELKKPDGSKI RQECLLGKP+NQWFEIVVEFKVGNH
Sbjct: 121 IVYEVALQVQLNDRASGWDAPLNIELKKPDGSKIVRQECLLGKPRNQWFEIVVEFKVGNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEFAF+EHGGHWKRGLLVKGVRIGAKGCGC+
Sbjct: 181 GCGSSGEIEFAFYEHGGHWKRGLLVKGVRIGAKGCGCS 216

BLAST of CmaCh13G004080 vs. TrEMBL
Match: Q8LK67_9ROSI (Phloem lectin OS=Cucurbita argyrosperma subsp. sororia GN=PP2 PE=2 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 1.5e-114
Identity = 199/218 (91.28%), Postives = 210/218 (96.33%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           M++KEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF
Sbjct: 1   MNHKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC  + +AELI+VSWLDIRGKINESMLS N
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC--NKIAELIQVSWLDIRGKINESMLSPN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           +VYEVALQVQLNS ASGWN P+NIELKKP+GSKI RQECLLGKP+NQWFEIV+EFKV NH
Sbjct: 121 IVYEVALQVQLNSGASGWNHPMNIELKKPNGSKIERQECLLGKPKNQWFEIVIEFKVDNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEF+FFEHGGHWKRGLLVKGVRIGAKGCGCA
Sbjct: 181 GCGSSGEIEFSFFEHGGHWKRGLLVKGVRIGAKGCGCA 216

BLAST of CmaCh13G004080 vs. TAIR10
Match: AT4G19840.1 (AT4G19840.1 phloem protein 2-A1)

HSP 1 Score: 122.1 bits (305), Expect = 4.3e-28
Identity = 76/207 (36.71%), Postives = 116/207 (56.04%), Query Frame = 1

Query: 16  VKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLN-KGAIKYIFDKKSNSNWYFIFAR 75
           VK  H  + IL++AD  +   S + L +QL +G+ L  K  IKY  D++ NSN + +FA+
Sbjct: 43  VKSPHNCEAILRDADPPISLSS-VNLSEQLRSGVFLKPKKQIKYWVDER-NSNCFMLFAK 102

Query: 76  ALSIAWIEDKRYWKWGSCGDS-----NVAELIEVSWLDIRGKINESMLSQNVVYEVALQV 135
            LSI W +D  YW W +  +S         L  V WLDI GK +   L+  +VYEV  +V
Sbjct: 103 NLSITWSDDVNYWTWFTEKESPNENVEAVGLKNVCWLDITGKFDTRNLTPGIVYEVVFKV 162

Query: 136 QLNSRASGWNAPLNIELKKPDGSKIARQE--CLLGKPQNQWFEIVVEFKVGNHGCGSSGE 195
           +L   A GW+ P+N++L  P+G +  +++   L   P+ +W ++ V   V      ++GE
Sbjct: 163 KLEDPAYGWDTPVNLKLVLPNGKEKPQEKKVSLRELPRYKWVDVRVGEFVPEK--SAAGE 222

Query: 196 IEFAFFEH-GGHWKRGLLVKGVRIGAK 214
           I F+ +EH  G WK+GL +KGV I  K
Sbjct: 223 ITFSMYEHAAGVWKKGLSLKGVAIRPK 245

BLAST of CmaCh13G004080 vs. TAIR10
Match: AT1G33920.1 (AT1G33920.1 phloem protein 2-A4)

HSP 1 Score: 93.2 bits (230), Expect = 2.1e-19
Identity = 60/156 (38.46%), Postives = 77/156 (49.36%), Query Frame = 1

Query: 69  YFIFARALSIAWIEDKRYWKW-------GSCGDSNVAELIEVSWLDIRGKINESMLSQNV 128
           + I+AR LSIAW +   YW W        S    + A L  V WLD+ GK +   L+   
Sbjct: 12  HMIYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELTLET 71

Query: 129 VYEVALQVQLNSRASGWNAPLNIELKKPDGSK--IARQECLLGKPQNQWFEIVV-EFKVG 188
            YEV   V+L   ASGWN P+N++L  PDG K    R  CL      +W +I   EF   
Sbjct: 72  TYEVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEFVTS 131

Query: 189 NHGCGSSGEIEFAFFE-HGGHWKRGLLVKGVRIGAK 214
                ++GEI F+ +E     WKRGL VK V I  K
Sbjct: 132 P---DNAGEISFSMYETKSCCWKRGLFVKCVEIRPK 164

BLAST of CmaCh13G004080 vs. TAIR10
Match: AT1G80110.1 (AT1G80110.1 phloem protein 2-B11)

HSP 1 Score: 80.1 bits (196), Expect = 1.9e-15
Identity = 60/196 (30.61%), Postives = 93/196 (47.45%), Query Frame = 1

Query: 36  PSFLKLYDQLVAG-ILLNKGAIKYIFDKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGD 95
           P+  +L+  LV   +L+N   + +  ++KS +  Y + ARAL+I W  ++RYW W S  +
Sbjct: 60  PTRKQLFFSLVDNPLLINGTLLSFSLERKSGNKCYMMAARALNIVWGHEQRYWHWISLPN 119

Query: 96  SN---VAELIEVSWLDIRGKINESMLSQNVVYEVALQVQLNSRASGWNAPLNIELKKPDG 155
           +    VAELI V WL+I GKIN ++LS + +Y      + N    G+  P+   L   D 
Sbjct: 120 TRFGEVAELIMVWWLEITGKINITLLSDDTLYAAYFVFKWNHSPYGFRQPVETSLVLADT 179

Query: 156 SKIAR--QECLLGKPQNQ--------------WFEIVVEFKVGNHGCGSSGEIEFAFFE- 211
                  Q  ++   Q+               W+E  VE        G  GEIE +  E 
Sbjct: 180 ESTDNVVQPSMISLMQDSGGEEGQSPVLRRDGWYE--VELGQFFKRRGDLGEIEMSLKET 239

BLAST of CmaCh13G004080 vs. TAIR10
Match: AT4G19850.2 (AT4G19850.2 lectin-related)

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-14
Identity = 47/137 (34.31%), Postives = 71/137 (51.82%), Query Frame = 1

Query: 63  KSNSNWYFIFARALSIAWIEDK--RYWKWGSCGD-------SNVAELIEVSWLDIRGKIN 122
           K+  N + ++AR LSI W E +  +YW W S  D       + VA++  V+WL++ GK  
Sbjct: 59  KAKKNCFMLYARDLSITWAESQTNKYWSWFSDLDQTSSDVRTEVAKMERVAWLEVVGKFE 118

Query: 123 ESMLSQNVVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVV 182
              L+ N +YEV   V+L   A GW+  +N +L  P G    R+E +    +N+W EI  
Sbjct: 119 TEKLTPNSLYEVVFVVKLIDSAKGWDFRVNFKLVLPTGETKERRENVNLLERNKWVEIPA 178

Query: 183 -EFKVGNHGCGSSGEIE 190
            EF +       SG+IE
Sbjct: 179 GEFMISPEHL--SGKIE 193

BLAST of CmaCh13G004080 vs. TAIR10
Match: AT1G65390.1 (AT1G65390.1 phloem protein 2 A5)

HSP 1 Score: 75.9 bits (185), Expect = 3.5e-14
Identity = 53/162 (32.72%), Postives = 75/162 (46.30%), Query Frame = 1

Query: 63  KSNSNWYFIFARALSIAWIEDKRYWKWGSCGDS-------NVAELIEVSWLDIRGKINES 122
           +   N + I AR LSIAW ED  +W W    +         +A L   SWLD+ GK +  
Sbjct: 253 RPKGNVFMIDARDLSIAWSEDSNHWTWLPLPNQNSNESVMEIAFLKSASWLDVAGKFDTR 312

Query: 123 MLSQNVVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQEC--LLGKPQNQWFEIVV 182
            L+    YEV   V+L      W   + ++L  P+  +  +++   +     +QW +I V
Sbjct: 313 YLTPRTRYEVVFVVKLEYTFE-WETLVKLKLDLPNTWEKPQEQSVDMFDYISDQWLDIPV 372

Query: 183 -EFKVGNHGCGSSGEIEFAFFEHGGH-WKRGLLVKGVRIGAK 214
            EF        + GEI FA +EH    WK GL VKGV I  K
Sbjct: 373 GEFTTSKK---NVGEISFAMYEHECQLWKSGLFVKGVTIRPK 410

BLAST of CmaCh13G004080 vs. NCBI nr
Match: gi|18069|emb|CAA78979.1| (dimeric phloem specific lectin PP2 [Cucurbita maxima])

HSP 1 Score: 456.8 bits (1174), Expect = 2.1e-125
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA
Sbjct: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 218

BLAST of CmaCh13G004080 vs. NCBI nr
Match: gi|21952274|gb|AAM82559.1|AF520583_1 (phloem protein 2 [Cucurbita digitata])

HSP 1 Score: 449.5 bits (1155), Expect = 3.3e-123
Identity = 215/218 (98.62%), Postives = 216/218 (99.08%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           VVYEVALQVQLNSRASGWNAPLNIELKKPDGS+IARQECLLGKPQNQWFEIVVEFKVGNH
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSRIARQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEFAFFEHGG W RGLLVKGVRIGAKGCGCA
Sbjct: 181 GCGSSGEIEFAFFEHGGAWARGLLVKGVRIGAKGCGCA 218

BLAST of CmaCh13G004080 vs. NCBI nr
Match: gi|410437|emb|CAA80364.1| (PP2 lectin polypeptide [Cucurbita maxima])

HSP 1 Score: 440.3 bits (1131), Expect = 2.0e-120
Identity = 209/218 (95.87%), Postives = 214/218 (98.17%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF
Sbjct: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKK NS+WYFIFARALSIAWIEDKRYWKWGSCG+S VAELIEVSWL+IRGKINESMLS N
Sbjct: 61  DKKLNSHWYFIFARALSIAWIEDKRYWKWGSCGNSEVAELIEVSWLNIRGKINESMLSPN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKI RQECLLGKPQNQWFEIVVEFKVGNH
Sbjct: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIVRQECLLGKPQNQWFEIVVEFKVGNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEF+FFEHGGHWKRGLLVKGVRIGAKGCGC+
Sbjct: 181 GCGSSGEIEFSFFEHGGHWKRGLLVKGVRIGAKGCGCS 218

BLAST of CmaCh13G004080 vs. NCBI nr
Match: gi|8307830|gb|AAF74345.1|AF150627_1 (phloem protein 2 [Cucurbita moschata])

HSP 1 Score: 427.2 bits (1097), Expect = 1.8e-116
Identity = 202/218 (92.66%), Postives = 211/218 (96.79%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           MDNKE+EAREKLGGEVKLGHCLDVILKNADVALHYPSF+KLYDQLVAGILLNKGAI+YIF
Sbjct: 1   MDNKEREAREKLGGEVKLGHCLDVILKNADVALHYPSFVKLYDQLVAGILLNKGAIRYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC    +AELIEVSWLDIRGKINESMLS N
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC--IKIAELIEVSWLDIRGKINESMLSPN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           +VYEVALQVQLN RASGW+APLNIELKKPDGSKI RQECLLGKP+NQWFEIVVEFKVGNH
Sbjct: 121 IVYEVALQVQLNDRASGWDAPLNIELKKPDGSKIVRQECLLGKPRNQWFEIVVEFKVGNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEFAF+EHGGHWKRGLLVKGVRIGAKGCGC+
Sbjct: 181 GCGSSGEIEFAFYEHGGHWKRGLLVKGVRIGAKGCGCS 216

BLAST of CmaCh13G004080 vs. NCBI nr
Match: gi|21952272|gb|AAM82558.1|AF520582_1 (phloem lectin [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 420.2 bits (1079), Expect = 2.1e-114
Identity = 199/218 (91.28%), Postives = 210/218 (96.33%), Query Frame = 1

Query: 1   MDNKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60
           M++KEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF
Sbjct: 1   MNHKEKEAREKLGGEVKLGHCLDVILKNADVALHYPSFLKLYDQLVAGILLNKGAIKYIF 60

Query: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSCGDSNVAELIEVSWLDIRGKINESMLSQN 120
           DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC  + +AELI+VSWLDIRGKINESMLS N
Sbjct: 61  DKKSNSNWYFIFARALSIAWIEDKRYWKWGSC--NKIAELIQVSWLDIRGKINESMLSPN 120

Query: 121 VVYEVALQVQLNSRASGWNAPLNIELKKPDGSKIARQECLLGKPQNQWFEIVVEFKVGNH 180
           +VYEVALQVQLNS ASGWN P+NIELKKP+GSKI RQECLLGKP+NQWFEIV+EFKV NH
Sbjct: 121 IVYEVALQVQLNSGASGWNHPMNIELKKPNGSKIERQECLLGKPKNQWFEIVIEFKVDNH 180

Query: 181 GCGSSGEIEFAFFEHGGHWKRGLLVKGVRIGAKGCGCA 219
           GCGSSGEIEF+FFEHGGHWKRGLLVKGVRIGAKGCGCA
Sbjct: 181 GCGSSGEIEFSFFEHGGHWKRGLLVKGVRIGAKGCGCA 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
LEC_LUFAC4.0e-4447.39Lectin OS=Luffa acutangula PE=1 SV=1[more]
P2A01_ARATH7.6e-2736.71Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana GN=PP2A1 PE=2 SV=1[more]
P2A04_ARATH3.8e-1838.46Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana GN=PP2A... [more]
P2B11_ARATH3.3e-1430.61F-box protein PP2-B11 OS=Arabidopsis thaliana GN=PP2B11 PE=1 SV=1[more]
P2A02_ARATH6.2e-1331.76Protein PHLOEM PROTEIN 2-LIKE A2 OS=Arabidopsis thaliana GN=PP2A2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
Q42383_CUCMA1.4e-125100.00Dimeric phloem specific lectin PP2 OS=Cucurbita maxima GN=PP2 PE=2 SV=1[more]
Q8LK66_9ROSI2.3e-12398.62Phloem protein 2 OS=Cucurbita digitata GN=PP2 PE=2 SV=1[more]
Q7DLM2_CUCMA1.4e-12095.87PP2 lectin polypeptide OS=Cucurbita maxima PE=2 SV=1[more]
Q9LLT3_CUCMO1.2e-11692.66Phloem protein 2 OS=Cucurbita moschata GN=pp2 PE=2 SV=1[more]
Q8LK67_9ROSI1.5e-11491.28Phloem lectin OS=Cucurbita argyrosperma subsp. sororia GN=PP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G19840.14.3e-2836.71 phloem protein 2-A1[more]
AT1G33920.12.1e-1938.46 phloem protein 2-A4[more]
AT1G80110.11.9e-1530.61 phloem protein 2-B11[more]
AT4G19850.21.2e-1434.31 lectin-related[more]
AT1G65390.13.5e-1432.72 phloem protein 2 A5[more]
Match NameE-valueIdentityDescription
gi|18069|emb|CAA78979.1|2.1e-125100.00dimeric phloem specific lectin PP2 [Cucurbita maxima][more]
gi|21952274|gb|AAM82559.1|AF520583_13.3e-12398.62phloem protein 2 [Cucurbita digitata][more]
gi|410437|emb|CAA80364.1|2.0e-12095.87PP2 lectin polypeptide [Cucurbita maxima][more]
gi|8307830|gb|AAF74345.1|AF150627_11.8e-11692.66phloem protein 2 [Cucurbita moschata][more]
gi|21952272|gb|AAM82558.1|AF520582_12.1e-11491.28phloem lectin [Cucurbita argyrosperma subsp. sororia][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR025886PP2-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0030246 carbohydrate binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G004080.1CmaCh13G004080.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025886Phloem protein 2-likePFAMPF14299PP2coord: 69..210
score: 3.9
NoneNo IPR availablePANTHERPTHR32278FAMILY NOT NAMEDcoord: 1..210
score: 1.2
NoneNo IPR availablePANTHERPTHR32278:SF3SUBFAMILY NOT NAMEDcoord: 1..210
score: 1.2

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh13G004080Cla002004Watermelon (97103) v1cmawmB197
CmaCh13G004080CmoCh13G004320Cucurbita moschata (Rifu)cmacmoB218
CmaCh13G004080Cp4.1LG20g06350Cucurbita pepo (Zucchini)cmacpeB234
The following gene(s) are paralogous to this gene:

None