CmaCh13G003400 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh13G003400
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Subtilisin-like serine protease
Location	Cma_Chr13 : 3948503 .. 3950681 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGAAGAGGCCACCAAGTTTGCAAGTAAATACTAAACTATTTTAACTTTAATATATAAACATAATAATTAATAATAATTAATAATAATAATTTATGAGACAGAGCACCCAGAAGTAGCAGCAGTGTTGCCAAACAAAGCCAAAGAGTTGCACACAACTCATTCATGGGAGTTCATGCATTTGGAGAAGAATGGTTTTATTCTCCCTTTTTCTCCTTGGAGGAAGGCTAGATTTGGAAAAGATGTTATTATTGCCAATCTCGACACCGGTAAATATATATTATCTTTTACTTATTCATCATATAACTCGATCGCATTAACATGAAATATCTAATTTAGATCATAAAGGTTGAGTTCGATTGTGGATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCTGGTTCTATTTTAAACTTTAATTACCCTTCCATTGGGGTTCAGAACTTGACTGGGAGTGTCACCGTTACTAGAAGGTTGAAGAATGTTGGCAGGCCGGGGGTTTATAGAGTCAGAGTTCGACGACCGGAAGGAGTTAAGGTTTCAGTGAAGCCAAGAGTTCTGAAGTTTTGGAAGATTGGAGAGGAGAAGAGGTTTGAATTGACGATGACCGGAGCTGTGGCGGAGGGTCAAATTGGTTATGGTACGCTAATTTGGACCGACGGCAAGCACTTTGTTAAGAGTCCAATTGTGGTTTCTTCTGGCTTTTTCTGA

mRNA sequence

ATGAGGAAGAGGCCACCAAGTTTGCAAAACTTGACTGGGAGTGTCACCGTTACTAGAAGGTTGAAGAATGTTGGCAGGCCGGGGGTTTATAGAGTCAGAGTTCGACGACCGGAAGGAGTTAAGGTTTCAGTGAAGCCAAGAGTTCTGAAGTTTTGGAAGATTGGAGAGGAGAAGAGGTTTGAATTGACGATGACCGGAGCTGTGGCGGAGGGTCAAATTGGTTATGGTACGCTAATTTGGACCGACGGCAAGCACTTTGTTAAGAGTCCAATTGTGGTTTCTTCTGGCTTTTTCTGA

Coding sequence (CDS)

ATGAGGAAGAGGCCACCAAGTTTGCAAAACTTGACTGGGAGTGTCACCGTTACTAGAAGGTTGAAGAATGTTGGCAGGCCGGGGGTTTATAGAGTCAGAGTTCGACGACCGGAAGGAGTTAAGGTTTCAGTGAAGCCAAGAGTTCTGAAGTTTTGGAAGATTGGAGAGGAGAAGAGGTTTGAATTGACGATGACCGGAGCTGTGGCGGAGGGTCAAATTGGTTATGGTACGCTAATTTGGACCGACGGCAAGCACTTTGTTAAGAGTCCAATTGTGGTTTCTTCTGGCTTTTTCTGA

Protein sequence

MRKRPPSLQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF

Homology

[Close]

BLAST of CmaCh13G003400 vs. Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.4e-17
Identity = 46/88 (52.27%), Postives = 59/88 (67.05%), Query Frame = 1


Query: 7   SLQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 66
           ++ NLTGS+TVTR+LKNVG P  Y  R R P GV+VSV+P+ L F K GE K F++T+  
Sbjct: 687 TVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRP 746

Query: 67  -AVAEGQIGYGTLIWTDGKHFVKSPIVV 94
             V      +G L WTD  H+V+SPIVV
Sbjct: 747 LPVTPSGYVFGELTWTDSHHYVRSPIVV 774

[Close]

BLAST of CmaCh13G003400 vs. Swiss-Prot
Match: AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.2e-17
Identity = 47/91 (51.65%), Postives = 66/91 (72.53%), Query Frame = 1


Query: 7   SLQNLTGS-VTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMT 66
           ++ NLT S VTV+R +KNVGRP +Y V+V  P+GV V+VKP  L F K+GE+K F++ + 
Sbjct: 681 TVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILV 740

Query: 67  ---GAVAEGQIGYGTLIWTDGKHFVKSPIVV 94
              G VA+G + +G L+W+D KH V+SPIVV
Sbjct: 741 KSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770

[Close]

BLAST of CmaCh13G003400 vs. Swiss-Prot
Match: SBT1_SOYBN (Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 70.9 bits (172), Expect = 9.0e-12
Identity = 36/81 (44.44%), Postives = 47/81 (58.02%), Query Frame = 1


Query: 15  VTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTM--TGAVAEGQ 74
           VT+ R + NVG P  Y V  R P G  ++V P  L F KIGE K F++ +  + A    +
Sbjct: 706 VTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRK 765

Query: 75  IGYGTLIWTDGKHFVKSPIVV 94
             +G L WTDGKH V+SPI V
Sbjct: 766 YEFGDLRWTDGKHIVRSPITV 786

[Close]

BLAST of CmaCh13G003400 vs. Swiss-Prot
Match: SBT55_ARATH (Subtilisin-like protease SBT5.5 OS=Arabidopsis thaliana GN=SBT5.5 PE=3 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 9.9e-11
Identity = 41/99 (41.41%), Postives = 53/99 (53.54%), Query Frame = 1


Query: 7   SLQNLTGSVTVTRRLKNVGRPG----VYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFEL 66
           S+  L+G+VTVTR +  VGR G    VY    + P GV V  +P VL F KIG++KRF +
Sbjct: 703 SIPYLSGTVTVTRTVTCVGRTGNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNI 762

Query: 67  -------TMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVS 95
                    TG     +  +G   WTDG H V+S I VS
Sbjct: 763 IFTTQRYEFTGEARRDRYRFGWFSWTDGHHVVRSSIAVS 801

[Close]

BLAST of CmaCh13G003400 vs. Swiss-Prot
Match: CRSP_ARATH (CO(2)-response secreted protease OS=Arabidopsis thaliana GN=CRSP PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.9e-10
Identity = 35/85 (41.18%), Postives = 51/85 (60.00%), Query Frame = 1


Query: 13  GSVTVTRRLKNVGRPG--VYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGAVAE 72
           GS TVTR + NVG  G  VY V V  P G  + V P  L+F K GE+  +++ ++   + 
Sbjct: 679 GSKTVTRTVTNVGEDGEAVYTVSVETPPGFNIQVTPEKLQFTKDGEKLTYQVIVSATASL 738

Query: 73  GQIGYGTLIWTDGKHFVKSPIVVSS 96
            Q  +G L W++ K+ V+SPIV+SS
Sbjct: 739 KQDVFGALTWSNAKYKVRSPIVISS 763

[Close]

BLAST of CmaCh13G003400 vs. TrEMBL
Match: A0A0A0LVY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171040 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 7.4e-29
Identity = 66/91 (72.53%), Postives = 75/91 (82.42%), Query Frame = 1


Query: 8   LQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGA 67
           +QNLTGSVTVTR+LKNV  PGVY+ RVR P GVKV VKP+VLKF ++GEEK FELT+TG 
Sbjct: 667 VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD 726

Query: 68  VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 99
           V E Q+  G LIWTDGKHFV+SPIVVSS  F
Sbjct: 727 VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

[Close]

BLAST of CmaCh13G003400 vs. TrEMBL
Match: A0A0A0LYF1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171030 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 7.6e-26
Identity = 57/91 (62.64%), Postives = 73/91 (80.22%), Query Frame = 1


Query: 8   LQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGA 67
           +QNL  SVT+TR+LKNVG PGVY+ ++  P  V+VSVKPR LKF ++GEEK FELT++G 
Sbjct: 685 VQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELTVSGV 744

Query: 68  VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 99
           V + +  YG LIW+DG+HFV+SPIVVSSG F
Sbjct: 745 VPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775

[Close]

BLAST of CmaCh13G003400 vs. TrEMBL
Match: A0A164ZU78_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_019655 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.4e-19
Identity = 51/91 (56.04%), Postives = 66/91 (72.53%), Query Frame = 1


Query: 7   SLQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTM-- 66
           ++ NL GS+TVTR +KNVG PG Y+ R+ RP G+ V+VKP  LKF KIGEEK+F++TM  
Sbjct: 821 TVPNLKGSITVTRTVKNVGSPGTYKARIVRPPGITVAVKPTSLKFGKIGEEKKFKITMKL 880

Query: 67  TGAVAEGQIGYGTLIWTDGKHFVKSPIVVSS 96
               A     +G LIW+DGKHFV+SPIVV +
Sbjct: 881 KQRSAAKDYVFGKLIWSDGKHFVRSPIVVKA 911

[Close]

BLAST of CmaCh13G003400 vs. TrEMBL
Match: A0A0J8BGA7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g066150 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.8e-19
Identity = 49/92 (53.26%), Postives = 71/92 (77.17%), Query Frame = 1


Query: 7   SLQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 66
           ++  L+GSVTVTR +KNVG PGVY  RVR+P  V V+V P+++KF K+GEEK+F++T+T 
Sbjct: 675 TVPKLSGSVTVTRTVKNVGSPGVYVARVRQPLRVSVTVTPKMMKFEKVGEEKKFKVTLTS 734

Query: 67  AVAEG---QIGYGTLIWTDGKHFVKSPIVVSS 96
            V  G   +  +G L+W+DGKH+V+SPIVV++
Sbjct: 735 KVRGGKSEEYRFGELLWSDGKHYVRSPIVVAA 766

[Close]

BLAST of CmaCh13G003400 vs. TrEMBL
Match: G7J840_MEDTR (Subtilisin-like serine protease OS=Medicago truncatula GN=MTR_3g082200 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 2.4e-19
Identity = 50/91 (54.95%), Postives = 66/91 (72.53%), Query Frame = 1


Query: 7   SLQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFE--LTM 66
           ++ NL+GSVTVTR LKNVG PG Y V V+ P G+ +SVKP +L+F K+GEEKRFE  L +
Sbjct: 681 TVPNLSGSVTVTRTLKNVGAPGTYIVHVQSPSGITISVKPNILEFKKVGEEKRFEVKLKV 740

Query: 67  TGAVAEGQIGYGTLIWTDGKHFVKSPIVVSS 96
               A     +G +IW+DGKH+VKSP+VV +
Sbjct: 741 KKGKATKSYVFGKMIWSDGKHYVKSPLVVKA 771

[Close]

BLAST of CmaCh13G003400 vs. TAIR10
Match: AT5G59810.1 (AT5G59810.1 Subtilase family protein)

HSP 1 Score: 90.1 bits (222), Expect = 8.1e-19
Identity = 46/88 (52.27%), Postives = 59/88 (67.05%), Query Frame = 1


Query: 7   SLQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 66
           ++ NLTGS+TVTR+LKNVG P  Y  R R P GV+VSV+P+ L F K GE K F++T+  
Sbjct: 687 TVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRP 746

Query: 67  -AVAEGQIGYGTLIWTDGKHFVKSPIVV 94
             V      +G L WTD  H+V+SPIVV
Sbjct: 747 LPVTPSGYVFGELTWTDSHHYVRSPIVV 774

[Close]

BLAST of CmaCh13G003400 vs. TAIR10
Match: AT2G04160.1 (AT2G04160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 89.0 bits (219), Expect = 1.8e-18
Identity = 47/91 (51.65%), Postives = 66/91 (72.53%), Query Frame = 1


Query: 7   SLQNLTGS-VTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMT 66
           ++ NLT S VTV+R +KNVGRP +Y V+V  P+GV V+VKP  L F K+GE+K F++ + 
Sbjct: 681 TVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILV 740

Query: 67  ---GAVAEGQIGYGTLIWTDGKHFVKSPIVV 94
              G VA+G + +G L+W+D KH V+SPIVV
Sbjct: 741 KSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770

[Close]

BLAST of CmaCh13G003400 vs. TAIR10
Match: AT5G45640.1 (AT5G45640.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 67.4 bits (163), Expect = 5.6e-12
Identity = 41/99 (41.41%), Postives = 53/99 (53.54%), Query Frame = 1


Query: 7   SLQNLTGSVTVTRRLKNVGRPG----VYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFEL 66
           S+  L+G+VTVTR +  VGR G    VY    + P GV V  +P VL F KIG++KRF +
Sbjct: 654 SIPYLSGTVTVTRTVTCVGRTGNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNI 713

Query: 67  -------TMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVS 95
                    TG     +  +G   WTDG H V+S I VS
Sbjct: 714 IFTTQRYEFTGEARRDRYRFGWFSWTDGHHVVRSSIAVS 752

[Close]

BLAST of CmaCh13G003400 vs. TAIR10
Match: AT1G20160.1 (AT1G20160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 65.9 bits (159), Expect = 1.6e-11
Identity = 35/85 (41.18%), Postives = 51/85 (60.00%), Query Frame = 1


Query: 13  GSVTVTRRLKNVGRPG--VYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGAVAE 72
           GS TVTR + NVG  G  VY V V  P G  + V P  L+F K GE+  +++ ++   + 
Sbjct: 679 GSKTVTRTVTNVGEDGEAVYTVSVETPPGFNIQVTPEKLQFTKDGEKLTYQVIVSATASL 738

Query: 73  GQIGYGTLIWTDGKHFVKSPIVVSS 96
            Q  +G L W++ K+ V+SPIV+SS
Sbjct: 739 KQDVFGALTWSNAKYKVRSPIVISS 763

[Close]

BLAST of CmaCh13G003400 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 62.8 bits (151), Expect = 1.4e-10
Identity = 33/85 (38.82%), Postives = 50/85 (58.82%), Query Frame = 1


Query: 13  GSVTVTRRLKNVGRPGVYRVRVR-RPEGVKVSVKPRVLKFWKIGEEKRFELTMT--GAVA 72
           G+   TR + +VG  G Y V+V     GVK+SV+P VL F +  E+K + +T T   +  
Sbjct: 671 GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKP 730

Query: 73  EGQIGYGTLIWTDGKHFVKSPIVVS 95
            G   +G++ W+DGKH V SP+ +S
Sbjct: 731 SGSNSFGSIEWSDGKHVVGSPVAIS 755

[Close]

BLAST of CmaCh13G003400 vs. NCBI nr
Match: gi|659128687|ref|XP_008464322.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 134.8 bits (338), Expect = 8.1e-29
Identity = 65/91 (71.43%), Postives = 75/91 (82.42%), Query Frame = 1


Query: 8   LQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGA 67
           +QNLTGSVT+TR+LKNV  PGVY+ RV  P GVKV VKP+VLKF ++GEEKRFEL +TG 
Sbjct: 686 VQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVLVKPKVLKFERVGEEKRFELIITGD 745

Query: 68  VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 99
           V E Q+  G LIWTDGKHFV+SPIVVSSG F
Sbjct: 746 VPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

[Close]

BLAST of CmaCh13G003400 vs. NCBI nr
Match: gi|449443664|ref|XP_004139597.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 134.4 bits (337), Expect = 1.1e-28
Identity = 66/91 (72.53%), Postives = 75/91 (82.42%), Query Frame = 1


Query: 8   LQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGA 67
           +QNLTGSVTVTR+LKNV  PGVY+ RVR P GVKV VKP+VLKF ++GEEK FELT+TG 
Sbjct: 661 VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD 720

Query: 68  VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 99
           V E Q+  G LIWTDGKHFV+SPIVVSS  F
Sbjct: 721 VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 751

[Close]

BLAST of CmaCh13G003400 vs. NCBI nr
Match: gi|700209886|gb|KGN64982.1| (hypothetical protein Csa_1G171040 [Cucumis sativus])

HSP 1 Score: 134.4 bits (337), Expect = 1.1e-28
Identity = 66/91 (72.53%), Postives = 75/91 (82.42%), Query Frame = 1


Query: 8   LQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGA 67
           +QNLTGSVTVTR+LKNV  PGVY+ RVR P GVKV VKP+VLKF ++GEEK FELT+TG 
Sbjct: 667 VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD 726

Query: 68  VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 99
           V E Q+  G LIWTDGKHFV+SPIVVSS  F
Sbjct: 727 VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

[Close]

BLAST of CmaCh13G003400 vs. NCBI nr
Match: gi|659128619|ref|XP_008464289.1| (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 129.8 bits (325), Expect = 2.6e-27
Identity = 60/91 (65.93%), Postives = 74/91 (81.32%), Query Frame = 1


Query: 8   LQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGA 67
           +QNL GSV+VTR+LKNVG PGVYR ++  P GV VSVKPR LKF ++GEEK FELT+ G 
Sbjct: 684 VQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGV 743

Query: 68  VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 99
           V + ++GYG LIW+DG+H V+SPIVVSSG F
Sbjct: 744 VPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 774

[Close]

BLAST of CmaCh13G003400 vs. NCBI nr
Match: gi|778665004|ref|XP_011648463.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-25
Identity = 57/91 (62.64%), Postives = 73/91 (80.22%), Query Frame = 1


Query: 8   LQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGA 67
           +QNL  SVT+TR+LKNVG PGVY+ ++  P  V+VSVKPR LKF ++GEEK FELT++G 
Sbjct: 685 VQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELTVSGV 744

Query: 68  VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 99
           V + +  YG LIW+DG+HFV+SPIVVSSG F
Sbjct: 745 VPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775

The following BLAST results are available for this feature:

BLAST of CmaCh13G003400 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SBT54_ARATH	1.4e-17	52.27	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1	[more]
AIR3_ARATH	3.2e-17	51.65	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1	[more]
SBT1_SOYBN	9.0e-12	44.44	Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3	[more]
SBT55_ARATH	9.9e-11	41.41	Subtilisin-like protease SBT5.5 OS=Arabidopsis thaliana GN=SBT5.5 PE=3 SV=2	[more]
CRSP_ARATH	2.9e-10	41.18	CO(2)-response secreted protease OS=Arabidopsis thaliana GN=CRSP PE=2 SV=1	[more]

BLAST of CmaCh13G003400 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVY8_CUCSA	7.4e-29	72.53	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171040 PE=4 SV=1	[more]
A0A0A0LYF1_CUCSA	7.6e-26	62.64	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171030 PE=4 SV=1	[more]
A0A164ZU78_DAUCA	1.4e-19	56.04	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_019655 PE=4 SV=1	[more]
A0A0J8BGA7_BETVU	1.8e-19	53.26	Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g066150 PE=4 S...	[more]
G7J840_MEDTR	2.4e-19	54.95	Subtilisin-like serine protease OS=Medicago truncatula GN=MTR_3g082200 PE=4 SV=1	[more]

BLAST of CmaCh13G003400 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G59810.1	8.1e-19	52.27	Subtilase family protein	[more]
AT2G04160.1	1.8e-18	51.65	Subtilisin-like serine endopeptidase family protein	[more]
AT5G45640.1	5.6e-12	41.41	Subtilisin-like serine endopeptidase family protein	[more]
AT1G20160.1	1.6e-11	41.18	Subtilisin-like serine endopeptidase family protein	[more]
AT5G67360.1	1.4e-10	38.82	Subtilase family protein	[more]

BLAST of CmaCh13G003400 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659128687\|ref\|XP_008464322.1\|	8.1e-29	71.43	PREDICTED: subtilisin-like protease [Cucumis melo]	[more]
gi\|449443664\|ref\|XP_004139597.1\|	1.1e-28	72.53	PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]
gi\|700209886\|gb\|KGN64982.1\|	1.1e-28	72.53	hypothetical protein Csa_1G171040 [Cucumis sativus]	[more]
gi\|659128619\|ref\|XP_008464289.1\|	2.6e-27	65.93	PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease [Cucumis melo]	[more]
gi\|778665004\|ref\|XP_011648463.1\|	1.1e-25	62.64	PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR015500	Peptidase_S8_subtilisin-rel

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004252	serine-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh13G003400.1	CmaCh13G003400.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 7..93 score: 1.3
None	No IPR available	PANTHER	PTHR10795:SF3	SUBFAMILY NOT NAMED	coord: 7..93 score: 1.3

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh13G003400	Cucumber (Chinese Long) v3	cmacucB0253
CmaCh13G003400	Watermelon (97103) v2	cmawmbB208
CmaCh13G003400	Wax gourd	cmawgoB0260
CmaCh13G003400	Cucurbita maxima (Rimu)	cmacmaB211
CmaCh13G003400	Cucumber (Gy14) v1	cgycmaB0883
CmaCh13G003400	Cucurbita moschata (Rifu)	cmacmoB218
CmaCh13G003400	Cucurbita moschata (Rifu)	cmacmoB220
CmaCh13G003400	Wild cucumber (PI 183967)	cmacpiB212
CmaCh13G003400	Wild cucumber (PI 183967)	cmacpiB222
CmaCh13G003400	Cucumber (Chinese Long) v2	cmacuB210
CmaCh13G003400	Cucumber (Chinese Long) v2	cmacuB222
CmaCh13G003400	Watermelon (Charleston Gray)	cmawcgB180
CmaCh13G003400	Watermelon (97103) v1	cmawmB202
CmaCh13G003400	Cucurbita pepo (Zucchini)	cmacpeB209
CmaCh13G003400	Bottle gourd (USVL1VR-Ls)	cmalsiB204
CmaCh13G003400	Cucumber (Gy14) v2	cgybcmaB068
CmaCh13G003400	Cucumber (Gy14) v2	cgybcmaB078