CmaCh13G003100 (gene) Cucurbita maxima (Rimu)

NameCmaCh13G003100
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPectin lyase-like superfamily protein, putative
LocationCma_Chr13 : 3343446 .. 3348557 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGCATGGAAGGATGCATGCACGTCCAATCGTTCTAGTGCGATTGTTATTCCGGGAGGGATATATAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATTGATAGATTGACATTGTCCGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTCGAAAAAGAATGATTGCCATAAACACATAAATTGTGCCCAACTTCCCATTGTAAGCAATATGCCTTCACCTTTCCTTCTCAATATCGTGCATCTTGTTGTTTTTAGATGTCTTCTCTAATCTTCTAACATTTATAATTTGGTAAACGTTCTTCATTTTCTAGAGGATAATTTTTAACCATAATAAAAAATAAATAAAAAATCATCTAAAGGAGTGAAATTCTTTCCGAGTTTTCGATTTTATTGGGTTTCGTTAAAATTTTTGGCTTCATTACTGATAGCAAGAACTTCATTCGGTTAAATTCTTTCTCACAAAATATTATTTGATTATACAAAATGTTTAGAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCCATAATGTCAATATAACTGCACCAGAAAACAGTCCCAACACCGATGGAATACACATCGGTCGATCAAATAGGATCGCAATCACAATGTCCAAAATTGCAACCGGAGATGATTGCATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTCATCGTAAACAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATTGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGGTATTGATCGCATTCTCAAAGCTTTGATTGATTTTGTCACTATAACTTTCACAAATAAACCACTTACAGAAATTTGTTGTCTCATTTTTATTTTCAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTGTGCAACCCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAATGGTCCTATTACATCTCAGTGCACGAATGTGAGACCCATCATTTCAGGAAAACAGAACCCTAAAATCTGTTCATCTCCTTATGTTGTCCCATGAATTGTTGATTGTCATAGTACTTCAAACTAAGCGATAACCTTAATTAAAAGAAGACAGCTCTATCCATTTTAATTTCTGTCTTTTTAGTTTCTTTTGTTGTACTTTTCGAAATATAAATCATGGTCAAATGTTTGAAGGAAGTCTTGTGTCCTGAACAGTGTAACGTTTTTATTACTTCGACGTTATAAAATAAAATGGTTTTACTCTTGTTTTTTCGTTTTAGATTTGAATTCCGAATAAGACAATGTAACGTTAAGGACTATTATAAAAAAAAAAACCATTATTTCAAAAAAAAAAGAACAAACAAACAAACAAAATCTGTAACCATAAAGAATATCTAACTCCCCTCCCTATTGGCAACCCTAAACAGTTAAAAATTTTCGAGACCAAATCGATAAGTCAGATCGAATGTCCTAACTATATTCGTTGGATATCGCACGGATCCATCAATATTGTGAAAGGAGAATTGACATTAGAGGGGACTAATCAAGTGAGTCGTATCCTTAAATCTATATTTTTCGTCAATGGTGTACATTCGGTTGCAATTTTTAGACCAGTCTTGAAAAATAGTCTGTTTTAGATTAGAAAATTAAGTGACAATTATTAATCTGTATTCTAGGAATTTACATTTAATATCAAATTATCTAACTATTTATCTCAATTTCTAAACTGTTCTACCGTACTTAGCTTTACGAAAAATGTCGATAATCCTAGAAAAATTAAATATCAAAAGTAGCTAAATGTTCTAAGTGTTTACTCTACAAGAACTTATAAAAATTGGCCAAAATTCGTGGTAAATAAGGGCATAAAAAAAAACGACAAATATTGGAAGAGAGAGAAGATAATAAAAACTTTGTATCCATAAATGCTCTGTCTCATGTTTTCAAGCCTTAAGATATTTGTTGCTCGCGAGTTGAGATATCTTTTCAGACCTACCTCAGTTATTTGGATCATAAACTTATTCAACCCAAACAACCGTGGTTAAATTATGTTTAGAATGATATCTAAGAAGAAAAATGGATACGAAGATTTTTGGAAATGGAAAATGGTGCCTCATGTTGAAGCTTTGGGCCGGAGTTTGAAACTTGAATCCGGCAACTGATTCCCTTGACATTCCTCTGCATCACTGTGATATGGCTACATTTCCAACTTGTCATGAATTGCTTCTATGAGTGAAATTTGTCCGCACAAATTAACACGGTTCCTTTTAGCAATTTTTTTTTTCCTTACTCAGATGTCTCCTAAGATGTAACCTCATCCCTAGAATTTGGACCGGGATTTGTAATCCAAATTTGGCACCCGATGGCCCCCACAATCCCTATGCTTCTTCAATGATATGGTCATACTATTTACTTGTTTTGAATTGCCTCTAAAACTGAAAAGTATACCTAAAAATCACATTACCTATCTTTGGTGGCTCAATCATAAGATTCAAAAGTTAGGCATGTTTAGTTTGGAGCATCCTTCTATTAGGCGACATCTTGTAATTTTTTTAATAATCATTTGAATAAAGACAAAGTACGCTAAAAATATTTCATCGAGTTTGAAATTTCGTCCTACCCACCCCAAAAAAAAAAAAAAAAAAAAAAATCTAACATTGAAATCAATGTATCTCGAAGTGCATCTTGTAATAGGAACTTCATTTGTACAAGGCTAGCATTAATTCGTGGCCGTAAAGCTTGTTTCCTTCCAGAAAAAGTACTTCGATGGGTTTGGCCAATGCCTAGTTTCTTTGAAATTCAACTGTTCATAGGCGAGTGAGAGAGATATTGCAACCCAACCATTAATGAACAACTTAGCAAAACAATAAACAAGGTTTAGTTATCAATTATGTTCGGATTAAGAGAGGGAGATGGAACCATAACTTCTGTTAGTTCCATCCCAACTCCATTTCTATTCTTCAATTGGCATTATTAAATACATAAAGGTTCCAAATCTAAAACCAAATATCATTGTCTCTCACTAGTCTTCAAGGAATATACATAACAAAAGAAAAGAAAGCAAGGGCATCATGAGTACGATCAAATCGAGTTTCTTGGTTGCTACAATTTTGATGCTATTATTGTTAGATTTTACCGTCGATGCTCAATCTAGTGCCGATGTTGTTTTTCCTTTGAAAAGATATGGTAACATAGTGCCTGGTACTGACATCAGAAGGTAAATATACAGTATGAACATCAATATTATCACTCGTTGAGTTGATCGATTGTTTGCTAATTTTTGTTACTTGTGACATGTAGGCTTTAGCAAATGCATGGAAGGATGCATGCACGTCCAATCGTTCTAGTGCGATTGTTATTCCGGGAGGGATATATAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATTGATAGATTGACATTGTCAGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTGGAAAAAGAATGATTGCCATAAACACATAAATTGTGCCCAACTTCCCATTGTAAGCAATATGCCTTCACCTTTCCTTCTCAATCTCGTGCATCTTGTTGTTTTTAGATGTCTTCTCTAATCTTCTAACATTTATAATTTGGTAAACGTTCTTCATTTTCTAGAGGATAATCTTTAACCATAATAAAAAATAAATAAAAAATCATCTAAAGGAGTGAAATTCTTTCCGAGTTTTCGATTTTATTGGGTTTCGTTAAAATTTTTGGCTTCATTACTGATAGCAAGAACTTCATTCGGTTAAATTCTTTCTCACAAAATATTATTTGATTATACAAAATGTTTAGAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCCATAATGTCAATATAACTGCACCAGAAAACAGTCCCAACACCGATGGAATACACATCGGTCGATCAAATAGGATCGCAATCACAATGTCCAAAATTGCAACCGGAGATGATTGCATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTCATCGTAAACAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATTGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGGTATTGATCGCATTCTCAAAGCTTTGATTGATTTTGTCACTATAACTTTCACAAATAAACCACTTACAGAAATTTGTTGTCTCATTTTTATTTTCAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTGTGCAACCCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAATGGTCCTATTACATCTCAGTGCACGAATGTGAGACCCATCATTTCAGGAAAACAGAACCCTAAAATC

mRNA sequence

AATGCATGGAAGGATGCATGCACGTCCAATCGTTCTAGTGCGATTGTTATTCCGGGAGGGATATATAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATTGATAGATTGACATTGTCCGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTCGAAAAAGAATGATTGCCATAAACACATAAATTGTGCCCAACTTCCCATTAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCCATAATGTCAATATAACTGCACCAGAAAACAGTCCCAACACCGATGGAATACACATCGGTCGATCAAATAGGATCGCAATCACAATGTCCAAAATTGCAACCGGAGATGATTGCATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTCATCGTAAACAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATTGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTGTGCAACCCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAATGGTCCTATTACATCTCAGTGCACGAATGCTTTAGCAAATGCATGGAAGGATGCATGCACGTCCAATCGTTCTAGTGCGATTGTTATTCCGGGAGGGATATATAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATTGATAGATTGACATTGTCAGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTGGAAAAAGAATGATTGCCATAAACACATAAATTGTGCCCAACTTCCCATTAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCCATAATGTCAATATAACTGCACCAGAAAACAGTCCCAACACCGATGGAATACACATCGGTCGATCAAATAGGATCGCAATCACAATGTCCAAAATTGCAACCGGAGATGATTGCATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTCATCGTAAACAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATTGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTGTGCAACCCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAATGGTCCTATTACATCTCAGTGCACGAATGTGAGACCCATCATTTCAGGAAAACAGAACCCTAAAATC

Coding sequence (CDS)

AATGCATGGAAGGATGCATGCACGTCCAATCGTTCTAGTGCGATTGTTATTCCGGGAGGGATATATAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATTGATAGATTGACATTGTCCGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTCGAAAAAGAATGATTGCCATAAACACATAAATTGTGCCCAACTTCCCATTAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCCATAATGTCAATATAACTGCACCAGAAAACAGTCCCAACACCGATGGAATACACATCGGTCGATCAAATAGGATCGCAATCACAATGTCCAAAATTGCAACCGGAGATGATTGCATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTCATCGTAAACAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATTGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTGTGCAACCCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAATGGTCCTATTACATCTCAGTGCACGAATGCTTTAGCAAATGCATGGAAGGATGCATGCACGTCCAATCGTTCTAGTGCGATTGTTATTCCGGGAGGGATATATAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATTGATAGATTGACATTGTCAGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTGGAAAAAGAATGATTGCCATAAACACATAAATTGTGCCCAACTTCCCATTAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCCATAATGTCAATATAACTGCACCAGAAAACAGTCCCAACACCGATGGAATACACATCGGTCGATCAAATAGGATCGCAATCACAATGTCCAAAATTGCAACCGGAGATGATTGCATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTCATCGTAAACAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATTGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTGTGCAACCCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAATGGTCCTATTACATCTCAGTGCACGAATGTGAGACCCATCATTTCAGGAAAACAGAACCCTAAAATC

Protein sequence

NAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFEHIDRLTLSGEGVFDGQGKAASKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI
BLAST of CmaCh13G003100 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 2.1e-115
Identity = 202/352 (57.39%), Postives = 251/352 (71.31%), Query Frame = 1

Query: 329 TNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP---KSLQ 388
           T AL  AWK+AC S   S I++P G + V     +GPC S I  QL GTL+AP     ++
Sbjct: 3   TQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIK 62

Query: 389 GDNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHIN-CAQLPISLRFNFITNSIVKR 448
           G  WI    ID LT+ G GVFDGQGK+AW +NDCHK+   C  L ++LR   +TNSI++ 
Sbjct: 63  GLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILRD 122

Query: 449 ITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCI 508
           +T+LDSKNFH+NV+GC NLTF    I+A E S NTDGIHIGRS+ + I  ++I TGDDCI
Sbjct: 123 VTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDDCI 182

Query: 509 SLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPA 568
           SLGDGSK INITN+TCGPGHGIS+GSLGRY NEE V G+ V NCTI  + NGVRIKTWP 
Sbjct: 183 SLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPK 242

Query: 569 SAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCAT 628
           S   G A+ M F DI M +V  PILIDQ YCP+NQC  ++PS +K+SK+SFKNI+G+  T
Sbjct: 243 S-EPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTSTT 302

Query: 629 PVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 677
             A+KL+CS+  PC+ V++ DIDLTY+G  GP TS C N++P I GKQ P I
Sbjct: 303 KEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAI 353

BLAST of CmaCh13G003100 vs. Swiss-Prot
Match: PGLR_BRANA (Polygalacturonase OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 8.1e-112
Identity = 189/356 (53.09%), Postives = 249/356 (69.94%), Query Frame = 1

Query: 322 GPITSQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP 381
           GP  S  T A+  A+  AC +   S ++IP G +K+ E +  GPC SPI+F L G ++  
Sbjct: 29  GPPNSDITAAVLKAFTSACQAPAPSQVLIPKGDFKLGETVMTGPCKSPIEFTLQGNVKTD 88

Query: 382 K-SLQG-DNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITN 441
             S QG D W+ FE I+   L+G G FDG+G AAWK N+CHK   C +LPIS+RF+F+ N
Sbjct: 89  GGSTQGKDRWVVFEKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPISVRFDFVDN 148

Query: 442 SIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIAT 501
           + +K +TSLD+KNFH NV+   N+TF N+ I AP  SPNTDGIH+GR   + I  +KIAT
Sbjct: 149 AEIKDVTSLDAKNFHFNVISGKNMTFDNIKIIAPAESPNTDGIHLGRCEGVKILNTKIAT 208

Query: 502 GDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRI 561
           GDDCIS+GDG K + I  V CGPGHGIS+GSLGRY  E+ V  + V NCT+  T+NG+RI
Sbjct: 209 GDDCISVGDGMKNLLIEKVVCGPGHGISVGSLGRYGWEQDVTDITVKNCTLEGTSNGLRI 268

Query: 562 KTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR 621
           KTWP++A + TA  + F DII+  VSNPILIDQEYCPWNQCN+  PS IK+  ++F+NIR
Sbjct: 269 KTWPSAACTTTAAGIHFEDIILNKVSNPILIDQEYCPWNQCNKNKPSTIKLVDITFRNIR 328

Query: 622 GSCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 676
           G+     A+KL+CS+  PC+ V++GDI++ Y G +GP T +CTNV P + G QNPK
Sbjct: 329 GTSENKDAVKLLCSKGHPCENVEIGDINIEYTGPDGPPTFECTNVTPKLVGAQNPK 384

BLAST of CmaCh13G003100 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 4.5e-110
Identity = 191/373 (51.21%), Postives = 260/373 (69.71%), Query Frame = 1

Query: 307 EVKVGDIDLTYNGINGPITSQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPC 366
           E + G  D+T  G N    +  + AL NA+K+AC S   S IVIP G + + +   +GPC
Sbjct: 21  ESQTGVFDITKYGANS--NADISEALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPC 80

Query: 367 NSPIDFQLGGTLQAPKS---LQGDNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHI 426
            SP++ Q+  TL+AP     L+   W+T   +D+ T+SG G+ DGQ  AAW   +C +  
Sbjct: 81  KSPLELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAW---ECKQSK 140

Query: 427 NCAQLPISLRFNFITNSIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIH 486
            C +LP +L FN +TNS +K IT+LDSK+FH+NV  C NLTF   N++AP NSPNTDGIH
Sbjct: 141 KCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIH 200

Query: 487 IGRSNRIAITMSKIATGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGV 546
           + RS+ + IT S  +TGDDCIS+GD ++Q+ IT VTCGPGHGIS+GSLG   +E+PV GV
Sbjct: 201 VSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGV 260

Query: 547 IVNNCTIINTTNGVRIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRK 606
            V NCT  NT NGVRIKTWPAS   G   ++ F DII+ NVSNP++IDQ YCP+N+CN+ 
Sbjct: 261 FVRNCTFTNTDNGVRIKTWPAS-HPGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKD 320

Query: 607 IPSKIKISKVSFKNIRGSCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTN 666
           +PS++KISKVSF+NI+G+  T  A+ L+ S+ +PC+ ++VGDID+TY+G  GP  S C N
Sbjct: 321 LPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEGPAKSSCEN 380

Query: 667 VRPIISGKQNPKI 677
           ++P + GKQNP +
Sbjct: 381 IKPSLKGKQNPPV 387

BLAST of CmaCh13G003100 vs. Swiss-Prot
Match: PGLR2_PLAAC (Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.9e-108
Identity = 182/352 (51.70%), Postives = 246/352 (69.89%), Query Frame = 1

Query: 329 TNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCN-SPIDFQLGGTLQAP---KSL 388
           + A+  AWK AC S   S ++IP G Y + E   +GPC  S I FQ+ G ++AP      
Sbjct: 24  SQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIGFQIDGVVKAPADPSKF 83

Query: 389 QGDNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKR 448
           + D W++F  ID LT+SG G  DGQG+ AW KN+C K+ NC    ++LRF+F+ +++V+ 
Sbjct: 84  KSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAMNLRFDFLKHAMVRD 143

Query: 449 ITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCI 508
           ITSL+SK FHINVL C ++TF +V +TAP  S NTDGIH+G S  + IT +KIATGDDCI
Sbjct: 144 ITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCI 203

Query: 509 SLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPA 568
           S+G GS+ + IT V CGPGHGISIGSLGRY NE+ V G+ V  CT   T NGVR+KTWP 
Sbjct: 204 SIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWP- 263

Query: 569 SAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCAT 628
           ++  G AT++ F D+ M NV NP+++DQEYCP+ QC+R+ PS+IK+S ++F NIRG+   
Sbjct: 264 NSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRIKLSNINFNNIRGTSTG 323

Query: 629 PVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 677
            VA+ + CS  +PC  +K+G+I+L+Y G  GP TS C+NV+P  SGKQ P I
Sbjct: 324 KVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKPTFSGKQVPAI 374

BLAST of CmaCh13G003100 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 3.2e-108
Identity = 181/344 (52.62%), Postives = 242/344 (70.35%), Query Frame = 1

Query: 334 NAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP---KSLQGDNWI 393
           +AWK+AC S   S +VIP G Y + +   +GPC +PI+  + GT+QAP    + +  NW+
Sbjct: 51  DAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWV 110

Query: 394 TFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDS 453
            F  ++   + G G+FDGQG  A++KN C      ++LP+++RF+F+TN++++ ITS DS
Sbjct: 111 RFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDS 170

Query: 454 KNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGS 513
           K FHINV  C N+T   + I AP+ SPNTDGIH+G+S  + I  S I TGDDCIS+GDG+
Sbjct: 171 KLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGT 230

Query: 514 KQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGT 573
           K + I  +TCGPGHGISIGSLG++ NEEPVEG+ ++NCTI NT+NG RIKTWP     G 
Sbjct: 231 KNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHG-GA 290

Query: 574 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKL 633
            + + F DI M NVS+PILIDQ+YCPWN+C +   SK+K+S +SFKNIRG+ A P AIK 
Sbjct: 291 VSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKF 350

Query: 634 ICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNP 675
           ICS   PC  V++ DID+ +NG   P TSQC NV+PI SGK NP
Sbjct: 351 ICSGSSPCQNVELADIDIKHNGAE-PATSQCLNVKPITSGKLNP 392

BLAST of CmaCh13G003100 vs. TrEMBL
Match: A5BHX0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_002011 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 1.7e-169
Identity = 306/584 (52.40%), Postives = 395/584 (67.64%), Query Frame = 1

Query: 130 LTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQINITNVTCGP 189
           +T   V I A  +S NTDGIH+GRS  + I+ + I TGDD +S+GDGS+ IN+  VTCGP
Sbjct: 1   MTLQYVTINATRDSLNTDGIHMGRSTGVNISDAIIKTGDDSLSIGDGSQHINVEKVTCGP 60

Query: 190 GHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATNMQFLDIIMV 249
           GHGIS+GSLG+Y NEEPV GV V NCT+INT NG+R+KTWP S AS  AT++ F DIIM 
Sbjct: 61  GHGISVGSLGKYHNEEPVVGVTVKNCTLINTMNGIRVKTWPDSPAS-VATDLHFEDIIMN 120

Query: 250 NVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATP------------VAIKL 309
           NV NPILI+QEYCP++QC  K+PS++KIS VSF+ I     T             VA + 
Sbjct: 121 NVGNPILINQEYCPYDQCQAKVPSQVKISDVSFQGICSMSGTQYKSVSKEWGFTIVAYRF 180

Query: 310 ICSRHLPCDEVKV--------------------GD--IDLTYNGINGPITSQCTNALANA 369
             ++    + + +                    GD   D+T  G      +  + AL  A
Sbjct: 181 YQTKKRETENIGMKLNIAAISLLLLLTSVAEVSGDTYFDVTKYGAQADGKTDISQALLTA 240

Query: 370 WKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP----KSLQGDNWIT 429
           W+ AC S   S +VIP GIY + +   +GPC +PI+  + GT++AP    +      W+ 
Sbjct: 241 WEAACASPIVSRVVIPAGIYALGQVTIEGPCKAPINLIVKGTVRAPVDTSRFKPQAGWVA 300

Query: 430 FEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSK 489
           F+H+D+ TLSG GVFDGQG++ W +  C +   C QLPI+LRFNF+TNSI+K ITS DSK
Sbjct: 301 FQHLDQFTLSGYGVFDGQGQSVWGQK-CSRTKYCNQLPINLRFNFLTNSIIKDITSKDSK 360

Query: 490 NFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSK 549
            FHINVLGC N+TF  V ITAPE+S NTDGIHIGRS  + IT S I TGDDC+S+GDGS+
Sbjct: 361 QFHINVLGCRNITFFQVAITAPEDSQNTDGIHIGRSRGVNITHSTIQTGDDCVSIGDGSE 420

Query: 550 QINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTA 609
           QI+IT V CGPGHGIS+GSLG Y NE PV G+ V NCT+ +TTNGVR+KT+P S+  GTA
Sbjct: 421 QIDITKVNCGPGHGISVGSLGLYKNEAPVIGIRVKNCTLSDTTNGVRVKTYP-SSPQGTA 480

Query: 610 TNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLI 669
           T M F DI+M NVSNPI+IDQEYCP NQCN ++PS++K+S +SFKNIRG+ +T +A+ L+
Sbjct: 481 TEMHFHDIVMNNVSNPIIIDQEYCPHNQCNLQVPSRVKLSNISFKNIRGTTSTELAVNLV 540

Query: 670 CSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 676
           CS+  PC +V++G+IDL Y G  G  +SQC NV   + G Q PK
Sbjct: 541 CSKGAPCQKVELGNIDLKYTGPEGTASSQCKNVEATLWGTQQPK 581

BLAST of CmaCh13G003100 vs. TrEMBL
Match: A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 8.6e-161
Identity = 270/348 (77.59%), Postives = 303/348 (87.07%), Query Frame = 1

Query: 329 TNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDN 388
           T AL +AW DAC S+R SA+VIP G +KV+EG FKGPC SPI+F++ GTLQAPK   GD+
Sbjct: 47  TEALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDS 106

Query: 389 WITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSL 448
           WITF ++DR+ LSG GVFDGQGKA W+KNDCHK I+CA+LPISLRF+FITNSIV+RITSL
Sbjct: 107 WITFAYVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSL 166

Query: 449 DSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGD 508
           DSKNFH+N+LGCNNLTF  VNI APENSPNTDGIHIGRS  I+I  S+IATGDDCISLGD
Sbjct: 167 DSKNFHVNILGCNNLTFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGD 226

Query: 509 GSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAAS 568
           GSK+I + NV CGPGHGISIGSLG+Y NEEPVEGVIVNNCTIINTTNGVRIKTWPAS  +
Sbjct: 227 GSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVA 286

Query: 569 GTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAI 628
           G ATNM F DI MVNVSNPILIDQEYCPWNQCNR+IPS IKI+KVSFKNIRGS ATPVA+
Sbjct: 287 GIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAV 346

Query: 629 KLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 677
           KLICS +LPC+EVKV +IDL YNGI G ITS+C NV+PIISG QNP I
Sbjct: 347 KLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 394

BLAST of CmaCh13G003100 vs. TrEMBL
Match: F6HHK3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0057g00380 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.1e-142
Identity = 263/479 (54.91%), Postives = 333/479 (69.52%), Query Frame = 1

Query: 151 IGRSNRIAITMSKIATGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGV 210
           +GRS  + I+ + I TGDD +S+GDGS+ IN+  VTCGPGHGIS+GSLG+Y NEEPV GV
Sbjct: 1   MGRSTGVNISDAIIKTGDDSLSIGDGSQHINVEKVTCGPGHGISVGSLGKYHNEEPVVGV 60

Query: 211 IVNNCTIINTTNGVRIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRK 270
            V NCT+INT NG+R+KTWP S AS  AT++ F DIIM NV NPILI+QEYCP++QC  K
Sbjct: 61  TVKNCTLINTMNGIRVKTWPDSPAS-VATDLHFEDIIMNNVGNPILINQEYCPYDQCQAK 120

Query: 271 IPSKIKISKVSFKNIRGSCAT----------PVAIKL-ICSRHLPCDEVKVGDI------ 330
           +PS++KIS VSF+ I     T           + +KL I +  L      V ++      
Sbjct: 121 VPSQVKISDVSFQGICSMSGTFYQTKKRETENIGMKLNIAAISLLLLLTSVAEVSGDTYF 180

Query: 331 DLTYNGINGPITSQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQ 390
           D+T  G      +  + AL  AW+ AC S  +S +VIP GIY + +   +GPC +PI+  
Sbjct: 181 DVTKYGAQADGKTDISQALLTAWEAACASPIASRVVIPAGIYALGQVTIEGPCKAPINLI 240

Query: 391 LGGTLQAPKSLQG----DNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLP 450
           + GT++AP           W+ F+H+D+ TLSG GVFDGQG++ W +  C +   C QLP
Sbjct: 241 VKGTVRAPVDTSRFKPQAGWVAFQHLDQFTLSGYGVFDGQGQSVWGQK-CSRTKYCHQLP 300

Query: 451 ISLRFNFITNSIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNR 510
           I+LRFNF+TNSI+K ITS DSK FHINVLGC N+TF  V ITAPE+S NTDGIHIGRS  
Sbjct: 301 INLRFNFLTNSIIKDITSKDSKQFHINVLGCRNITFFQVAITAPEDSQNTDGIHIGRSRG 360

Query: 511 IAITMSKIATGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCT 570
           + IT S I TGDDC+S+GDGS+QI+IT V CGPGHGIS+GSLG Y NE PV G+ V NCT
Sbjct: 361 VNITHSTIQTGDDCVSIGDGSEQIDITKVNCGPGHGISVGSLGLYKNEAPVIGIRVKNCT 420

Query: 571 IINTTNGVRIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKI 609
           + +TTNGVR+KTWP S+  GTAT M F DI+M NVSNPI+IDQEYCP NQCN ++  +I
Sbjct: 421 LSDTTNGVRVKTWP-SSPQGTATEMHFHDIVMNNVSNPIIIDQEYCPHNQCNLQMLQRI 476

BLAST of CmaCh13G003100 vs. TrEMBL
Match: F6HHK2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0057g00370 PE=3 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 1.7e-132
Identity = 247/470 (52.55%), Postives = 317/470 (67.45%), Query Frame = 1

Query: 222 NGVRIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIK----- 281
           NG+R+KTWP S AS  AT++ F DIIM NV NPILI+QEYCP++QC  K  S  K     
Sbjct: 2   NGIRVKTWPDSPAS-VATDLHFEDIIMNNVGNPILINQEYCPYDQCQAKYKSVSKEWGFT 61

Query: 282 ISKVSFKNIRGSCATPVAIKL-ICSRHLPCDEVKVGDI------DLTYNGINGPITSQCT 341
           I    F   +      V +KL I +  L      V ++      D+T  G      +  +
Sbjct: 62  IVAYRFYQTKKREKENVGMKLNIAAISLLLLLASVAEVSGDTYFDVTKYGAQADGKTDIS 121

Query: 342 NALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQG--- 401
            AL  AW+ AC S  +S +VIP GIY + +   +GPC +PI+  + GT++AP        
Sbjct: 122 QALLTAWEAACASPIASRVVIPAGIYALGQVTIEGPCKAPINLIVKGTVRAPVDTSRFKP 181

Query: 402 -DNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRI 461
              W+ F+H+D+ TLSG GVFDGQG++ W++  C +   C QLPI+LRFNF+TNSI+K I
Sbjct: 182 QAGWVAFQHLDQFTLSGYGVFDGQGQSVWRQK-CSRTKYCHQLPINLRFNFLTNSIIKDI 241

Query: 462 TSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCIS 521
           TS DSK FHINVL C N+TF  V ITAPE+S NTDGIHIGRS  + IT S I TGDDC+S
Sbjct: 242 TSKDSKQFHINVLRCRNITFFQVAITAPEDSQNTDGIHIGRSRGVNITHSTIQTGDDCVS 301

Query: 522 LGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPAS 581
           +GDGS+QI+IT V CGPGHGIS+GSLG Y NE PV G+ V NCT+ +TTNGVR+KTWP S
Sbjct: 302 IGDGSEQIDITKVNCGPGHGISVGSLGLYKNEAPVIGIRVKNCTLSDTTNGVRVKTWP-S 361

Query: 582 AASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATP 641
           +  GTAT M F DI+M NVSNPI+IDQEYCP NQCN +IPS++K+S +SFKNIRG+ +T 
Sbjct: 362 SPQGTATEMHFHDIVMNNVSNPIIIDQEYCPHNQCNLQIPSRVKLSNISFKNIRGTTSTE 421

Query: 642 VAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 676
           +A+ L+CS+  PC +V++G+IDL Y G  G  +SQC NV   + G Q PK
Sbjct: 422 LAVNLVCSKGAPCQKVELGNIDLKYTGPEGTASSQCKNVEATLWGTQQPK 468

BLAST of CmaCh13G003100 vs. TrEMBL
Match: E3VSV7_CUCPE (Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 8.7e-129
Identity = 226/367 (61.58%), Postives = 277/367 (75.48%), Query Frame = 1

Query: 311 GDIDLTYNGINGPITSQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPI 370
           G  D+T  G      +  T ALA+AWK+AC S   S +++P G Y++ E   KGPC SPI
Sbjct: 38  GVFDITTFGAKP--NADATQALADAWKEACASATPSKVLVPKGTYQLGESRLKGPCKSPI 97

Query: 371 DFQLGGTLQA-PKSLQGDNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLP 430
           +  + GTLQA P   +GD  +  E+ID LTLSG GVFDGQGKA W+KNDCH    C +LP
Sbjct: 98  ELLVEGTLQASPTDTEGDGLLVMEYIDHLTLSGTGVFDGQGKAGWEKNDCHLKKECKKLP 157

Query: 431 ISLRFNFITNSIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNR 490
           ++L+FNFITNSIVK ITSLDSKNFHINVL   NLTF ++ ITAP NSPNTDGIH+G S  
Sbjct: 158 MNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKITAPHNSPNTDGIHLGDSED 217

Query: 491 IAITMSKIATGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCT 550
           + I  + IATGDDCIS+G  +++I I++VTCGPGHGISIGSLG+Y  E+ V GV V  C 
Sbjct: 218 VYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIGSLGKYKTEKEVVGVTVKKCK 277

Query: 551 IINTTNGVRIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIK 610
           +I TTNGVRIK+WP SA S  A++M F DI M NV+NPI+IDQEYCPWNQCNRK+PS IK
Sbjct: 278 LIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIIIDQEYCPWNQCNRKVPSAIK 337

Query: 611 ISKVSFKNIRGSCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIIS 670
           ISKVSFKNIRG+ +TPVA+KL+CS+  PC+ V++ DIDLTY+G  GP  S+C+NV+P IS
Sbjct: 338 ISKVSFKNIRGTSSTPVAVKLVCSKSNPCEGVEIADIDLTYSGKEGPAVSECSNVKPTIS 397

Query: 671 GKQNPKI 677
           GKQNP I
Sbjct: 398 GKQNPPI 402

BLAST of CmaCh13G003100 vs. TAIR10
Match: AT3G07820.1 (AT3G07820.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 419.5 bits (1077), Expect = 4.0e-117
Identity = 194/352 (55.11%), Postives = 260/352 (73.86%), Query Frame = 1

Query: 326 SQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP-KSL 385
           S  T AL  A+  AC S+  S +VIP G +K+ E   +GPC +PI+  L GT++A   ++
Sbjct: 33  SDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQGTVKADGNAI 92

Query: 386 QG-DNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVK 445
           QG + W+ F +ID   L+G G FDG+G AAW+ N+CHK   C +LPIS+RF+FI NS ++
Sbjct: 93  QGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIR 152

Query: 446 RITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDC 505
            I+S+D+KNFHINVLG  N+T +N+ I APE+SPNTDGIH+GRS+ + I  S I+TGDDC
Sbjct: 153 DISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDC 212

Query: 506 ISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWP 565
           IS+GDG K +++  VTCGPGHGIS+GSLGRY +E+ V G+ V NCT+  T NG+RIKTWP
Sbjct: 213 ISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWP 272

Query: 566 ASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCA 625
           ++A S TA+++ F DII+ +VSNPILIDQEYCPWNQCN++  S IK+  +SFKNIRG+  
Sbjct: 273 SAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSG 332

Query: 626 TPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 676
              A+KL+CS+  PC  V++GDID+ YNG +GP T  C+NV P I G Q+PK
Sbjct: 333 NKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQSPK 384

BLAST of CmaCh13G003100 vs. TAIR10
Match: AT5G48140.1 (AT5G48140.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 419.1 bits (1076), Expect = 5.2e-117
Identity = 192/357 (53.78%), Postives = 261/357 (73.11%), Query Frame = 1

Query: 320 INGPITSQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQ 379
           IN P  S  TNAL  A+ +AC     S ++IP G YK+ E +  GPC +PI   L GT++
Sbjct: 28  INSPPGSDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAPIRIALLGTVK 87

Query: 380 APKSLQG-DNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFIT 439
           A  +  G + W+ F +I+   L+G GVFDG+G AAW+ N+CHK  NC +LPIS+RF+F+T
Sbjct: 88  ADGNANGKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVT 147

Query: 440 NSIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIA 499
           ++ ++ ITSLD+K+FHINV+G  N+TF +V I AP  SPNTDGIH+GRS+ I I  S I+
Sbjct: 148 DAKIRGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFIS 207

Query: 500 TGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVR 559
           TGDDC+S+GDG K + +  VTCGPGHGISIGSLGRY++EE V G+ + NCT+  T NG+R
Sbjct: 208 TGDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLR 267

Query: 560 IKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNI 619
           IKTWP++A + TA+++ F +I++ NVSNPILIDQEYCPWNQCN++ PS IK++ +SFK I
Sbjct: 268 IKTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKI 327

Query: 620 RGSCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 676
           RG+     A+KL+CS+  PC  V+VGD+++ Y G +GP T QC+NV P + G Q PK
Sbjct: 328 RGTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPK 384

BLAST of CmaCh13G003100 vs. TAIR10
Match: AT3G07840.1 (AT3G07840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 412.5 bits (1059), Expect = 4.9e-115
Identity = 191/358 (53.35%), Postives = 258/358 (72.07%), Query Frame = 1

Query: 320 INGPITSQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQ 379
           + G   S  T AL  A+  AC +  +S +VI  G +K+ E    GPC +P++  L GTL+
Sbjct: 28  VGGSPGSDITQALLKAFTSACQAPTASKVVITKGEFKLGEIEMTGPCKAPVEINLQGTLK 87

Query: 380 AP-KSLQG-DNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFI 439
           A  K++QG + W+ F  I+   L+G G+FDG+G AAW+ N+CHK   C +LPIS+RF+F+
Sbjct: 88  ADGKAIQGKERWVVFLRINGFKLNGGGIFDGEGNAAWRVNNCHKTFECKKLPISIRFDFV 147

Query: 440 TNSIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKI 499
            N+ ++ I+S+D+KNFHINVLG  N+TF NV + AP  SPNTDGIH+GRS  + I  SKI
Sbjct: 148 ENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKI 207

Query: 500 ATGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGV 559
           ATGDDCIS+GDG K +++ NV CGPGHGIS+GSLGRY +E+ V G+ V NCT+  T NG+
Sbjct: 208 ATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGL 267

Query: 560 RIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKN 619
           RIKTWP++A + TA+ + F +II+ NVSNPILIDQEYCPWNQCN++ PS IK+  +SFKN
Sbjct: 268 RIKTWPSAACATTASGIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKN 327

Query: 620 IRGSCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 676
           IRG+     A+KL+CS+  PC  V++G+I+L Y G +GP T  C+NV P + G QNPK
Sbjct: 328 IRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGADGPPTFMCSNVSPKLVGTQNPK 385

BLAST of CmaCh13G003100 vs. TAIR10
Match: AT3G07830.1 (AT3G07830.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 407.1 bits (1045), Expect = 2.1e-113
Identity = 187/352 (53.12%), Postives = 254/352 (72.16%), Query Frame = 1

Query: 326 SQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP-KSL 385
           S  T AL  A+  AC S     +VIP G +K+ E +  GPC SP++  L GT+ A   S+
Sbjct: 34  SDITQALLRAFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPVEITLLGTVLADGNSI 93

Query: 386 QG-DNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVK 445
            G + W+ F+ +D   L+G G FDG+G AAW+ N+CHK   C +LPIS+RF+F+TN+ ++
Sbjct: 94  HGKEKWVVFQRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVTNAEIR 153

Query: 446 RITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDC 505
            I+S+D+KNFHINV+G  N+TF NV I AP  SPNTDGIH+GRS  ++I  S+I+TGDDC
Sbjct: 154 DISSIDAKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDC 213

Query: 506 ISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWP 565
           +S+GDG   + + NV CGPGHGIS+GSLGRY +E+ V G+ V NCT+  T NG+RIKTWP
Sbjct: 214 VSVGDGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWP 273

Query: 566 ASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCA 625
           ++A S TA+N+ F +II+ NVSNPILIDQEYCPWNQCN++  S IK++ +SF+ IRG+  
Sbjct: 274 SAACSTTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSIKLANISFRRIRGTSG 333

Query: 626 TPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 676
              A+KL+CS+  PC+ V+VGDI++ Y G +GP T  C+NVRP + G Q PK
Sbjct: 334 NKDAVKLLCSKGYPCENVQVGDINIQYTGADGPATFMCSNVRPKLVGTQFPK 385

BLAST of CmaCh13G003100 vs. TAIR10
Match: AT3G14040.1 (AT3G14040.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 386.3 bits (991), Expect = 3.8e-107
Identity = 186/367 (50.68%), Postives = 249/367 (67.85%), Query Frame = 1

Query: 313 IDLTYNGINGPITSQCTNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDF 372
           +D+  +G  G   +  + A A AWK+AC +   S I +P G Y V+   FKGPC  P+  
Sbjct: 71  LDVKASGAKGDGKTDDSAAFAAAWKEACAAG--STITVPKGEYLVESLEFKGPCKGPVTL 130

Query: 373 QLGGTLQAPKSLQGDN----WITFEHIDRLTLSG-EGVFDGQGKAAWKKNDCHKHINCAQ 432
           +L G  +AP +++       WI FE++   TL+G + +FDGQG  AWK NDC K   C  
Sbjct: 131 ELNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNS 190

Query: 433 LPISLRFNFITNSIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRS 492
           LPI++RF  +TNS +  ITS +SK FH+N+L C N+T  ++ I AP  S NTDGIHIGRS
Sbjct: 191 LPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRS 250

Query: 493 NRIAITMSKIATGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNN 552
           N + +  +KI TGDDC+S+GDG++ + + NV CGPGHGISIGSLGRY NE+PV+GV V  
Sbjct: 251 NGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRK 310

Query: 553 CTIINTTNGVRIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSK 612
           C I NT NGVRIKTWP S   G A+N+ F DI M NVS P+LIDQEYCP+  C   +PSK
Sbjct: 311 CLIKNTDNGVRIKTWPGS-PPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSK 370

Query: 613 IKISKVSFKNIRGSCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPI 672
           +K+S V+ KNI+G+ AT VA+KL+CS+ +PC  + + DI+L +NG  GP  S C+N++PI
Sbjct: 371 VKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPI 430

Query: 673 ISGKQNP 675
           +SGK  P
Sbjct: 431 LSGKLVP 434

BLAST of CmaCh13G003100 vs. NCBI nr
Match: gi|147835151|emb|CAN65676.1| (hypothetical protein VITISV_002011 [Vitis vinifera])

HSP 1 Score: 604.4 bits (1557), Expect = 2.5e-169
Identity = 306/584 (52.40%), Postives = 395/584 (67.64%), Query Frame = 1

Query: 130 LTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQINITNVTCGP 189
           +T   V I A  +S NTDGIH+GRS  + I+ + I TGDD +S+GDGS+ IN+  VTCGP
Sbjct: 1   MTLQYVTINATRDSLNTDGIHMGRSTGVNISDAIIKTGDDSLSIGDGSQHINVEKVTCGP 60

Query: 190 GHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATNMQFLDIIMV 249
           GHGIS+GSLG+Y NEEPV GV V NCT+INT NG+R+KTWP S AS  AT++ F DIIM 
Sbjct: 61  GHGISVGSLGKYHNEEPVVGVTVKNCTLINTMNGIRVKTWPDSPAS-VATDLHFEDIIMN 120

Query: 250 NVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATP------------VAIKL 309
           NV NPILI+QEYCP++QC  K+PS++KIS VSF+ I     T             VA + 
Sbjct: 121 NVGNPILINQEYCPYDQCQAKVPSQVKISDVSFQGICSMSGTQYKSVSKEWGFTIVAYRF 180

Query: 310 ICSRHLPCDEVKV--------------------GD--IDLTYNGINGPITSQCTNALANA 369
             ++    + + +                    GD   D+T  G      +  + AL  A
Sbjct: 181 YQTKKRETENIGMKLNIAAISLLLLLTSVAEVSGDTYFDVTKYGAQADGKTDISQALLTA 240

Query: 370 WKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP----KSLQGDNWIT 429
           W+ AC S   S +VIP GIY + +   +GPC +PI+  + GT++AP    +      W+ 
Sbjct: 241 WEAACASPIVSRVVIPAGIYALGQVTIEGPCKAPINLIVKGTVRAPVDTSRFKPQAGWVA 300

Query: 430 FEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSK 489
           F+H+D+ TLSG GVFDGQG++ W +  C +   C QLPI+LRFNF+TNSI+K ITS DSK
Sbjct: 301 FQHLDQFTLSGYGVFDGQGQSVWGQK-CSRTKYCNQLPINLRFNFLTNSIIKDITSKDSK 360

Query: 490 NFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSK 549
            FHINVLGC N+TF  V ITAPE+S NTDGIHIGRS  + IT S I TGDDC+S+GDGS+
Sbjct: 361 QFHINVLGCRNITFFQVAITAPEDSQNTDGIHIGRSRGVNITHSTIQTGDDCVSIGDGSE 420

Query: 550 QINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTA 609
           QI+IT V CGPGHGIS+GSLG Y NE PV G+ V NCT+ +TTNGVR+KT+P S+  GTA
Sbjct: 421 QIDITKVNCGPGHGISVGSLGLYKNEAPVIGIRVKNCTLSDTTNGVRVKTYP-SSPQGTA 480

Query: 610 TNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLI 669
           T M F DI+M NVSNPI+IDQEYCP NQCN ++PS++K+S +SFKNIRG+ +T +A+ L+
Sbjct: 481 TEMHFHDIVMNNVSNPIIIDQEYCPHNQCNLQVPSRVKLSNISFKNIRGTTSTELAVNLV 540

Query: 670 CSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 676
           CS+  PC +V++G+IDL Y G  G  +SQC NV   + G Q PK
Sbjct: 541 CSKGAPCQKVELGNIDLKYTGPEGTASSQCKNVEATLWGTQQPK 581

BLAST of CmaCh13G003100 vs. NCBI nr
Match: gi|659111053|ref|XP_008455553.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 584.3 bits (1505), Expect = 2.7e-163
Identity = 273/348 (78.45%), Postives = 307/348 (88.22%), Query Frame = 1

Query: 329 TNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDN 388
           T AL +AW DAC S R SA+VIP G +KV+EG FKGPC SP++ ++ GTLQAPK   GD+
Sbjct: 48  TEALKDAWNDACASLRPSAVVIPKGTFKVREGEFKGPCKSPVEVRVRGTLQAPKHPHGDS 107

Query: 389 WITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSL 448
           WITF ++DR+TLSG GVFDGQGKA W+KNDCHKHI+CA+LPISLRFNFITNSIV+RITSL
Sbjct: 108 WITFAYVDRMTLSGGGVFDGQGKAGWEKNDCHKHISCARLPISLRFNFITNSIVRRITSL 167

Query: 449 DSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGD 508
           DSKNFH+N+LGCNNLTF  VNI APE+SPNTDGIHIGRS  I+I  S+IATGDDCISLGD
Sbjct: 168 DSKNFHVNILGCNNLTFDGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGD 227

Query: 509 GSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAAS 568
           GSK++ ITNVTCGPGHGISIGSLG+YTNEEPVEGVIV NCTI+NTTNGVRIKTWPAS  +
Sbjct: 228 GSKRVKITNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNGVRIKTWPASPVA 287

Query: 569 GTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAI 628
           G ATNM FLDI MVNVSNPILIDQEYCPWNQCNRKIPSKIKI+KVSFKNIRGS ATPVA+
Sbjct: 288 GIATNMHFLDITMVNVSNPILIDQEYCPWNQCNRKIPSKIKINKVSFKNIRGSSATPVAV 347

Query: 629 KLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 677
           KLICS +LPC+EVKV +IDL YNGI G +TS+C NV+PIISG QNP I
Sbjct: 348 KLICSSNLPCEEVKVANIDLVYNGIKGSLTSECMNVKPIISGTQNPPI 395

BLAST of CmaCh13G003100 vs. NCBI nr
Match: gi|778730103|ref|XP_011659699.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 575.5 bits (1482), Expect = 1.2e-160
Identity = 270/348 (77.59%), Postives = 303/348 (87.07%), Query Frame = 1

Query: 329 TNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDN 388
           T AL +AW DAC S+R SA+VIP G +KV+EG FKGPC SPI+F++ GTLQAPK   GD+
Sbjct: 32  TEALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDS 91

Query: 389 WITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSL 448
           WITF ++DR+ LSG GVFDGQGKA W+KNDCHK I+CA+LPISLRF+FITNSIV+RITSL
Sbjct: 92  WITFAYVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSL 151

Query: 449 DSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGD 508
           DSKNFH+N+LGCNNLTF  VNI APENSPNTDGIHIGRS  I+I  S+IATGDDCISLGD
Sbjct: 152 DSKNFHVNILGCNNLTFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGD 211

Query: 509 GSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAAS 568
           GSK+I + NV CGPGHGISIGSLG+Y NEEPVEGVIVNNCTIINTTNGVRIKTWPAS  +
Sbjct: 212 GSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVA 271

Query: 569 GTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAI 628
           G ATNM F DI MVNVSNPILIDQEYCPWNQCNR+IPS IKI+KVSFKNIRGS ATPVA+
Sbjct: 272 GIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAV 331

Query: 629 KLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 677
           KLICS +LPC+EVKV +IDL YNGI G ITS+C NV+PIISG QNP I
Sbjct: 332 KLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 379

BLAST of CmaCh13G003100 vs. NCBI nr
Match: gi|700188231|gb|KGN43464.1| (hypothetical protein Csa_7G037910 [Cucumis sativus])

HSP 1 Score: 575.5 bits (1482), Expect = 1.2e-160
Identity = 270/348 (77.59%), Postives = 303/348 (87.07%), Query Frame = 1

Query: 329 TNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDN 388
           T AL +AW DAC S+R SA+VIP G +KV+EG FKGPC SPI+F++ GTLQAPK   GD+
Sbjct: 47  TEALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDS 106

Query: 389 WITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSL 448
           WITF ++DR+ LSG GVFDGQGKA W+KNDCHK I+CA+LPISLRF+FITNSIV+RITSL
Sbjct: 107 WITFAYVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSL 166

Query: 449 DSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGD 508
           DSKNFH+N+LGCNNLTF  VNI APENSPNTDGIHIGRS  I+I  S+IATGDDCISLGD
Sbjct: 167 DSKNFHVNILGCNNLTFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGD 226

Query: 509 GSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAAS 568
           GSK+I + NV CGPGHGISIGSLG+Y NEEPVEGVIVNNCTIINTTNGVRIKTWPAS  +
Sbjct: 227 GSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVA 286

Query: 569 GTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAI 628
           G ATNM F DI MVNVSNPILIDQEYCPWNQCNR+IPS IKI+KVSFKNIRGS ATPVA+
Sbjct: 287 GIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAV 346

Query: 629 KLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 677
           KLICS +LPC+EVKV +IDL YNGI G ITS+C NV+PIISG QNP I
Sbjct: 347 KLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 394

BLAST of CmaCh13G003100 vs. NCBI nr
Match: gi|449445367|ref|XP_004140444.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 501.1 bits (1289), Expect = 3.0e-138
Identity = 234/348 (67.24%), Postives = 279/348 (80.17%), Query Frame = 1

Query: 329 TNALANAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDN 388
           T+AL NAWK AC S   S ++IP GIY + +   KGPC S I+ Q+ GTLQAP   +GD 
Sbjct: 41  THALLNAWKGACASTSRSKVLIPKGIYLLSQSNLKGPCKSSIEIQVQGTLQAPPDPKGDG 100

Query: 389 WITFEHIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSL 448
            +  E+ID+LTLSG GVFDGQGKA W+KNDCHK   C +LP++L+FNF+TNSIVK ITSL
Sbjct: 101 LVILEYIDQLTLSGTGVFDGQGKAGWEKNDCHKKKICTKLPMTLKFNFVTNSIVKDITSL 160

Query: 449 DSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGD 508
           DSKNFHIN+LGC N+TF +V I+APENSPNTDGIHI  S +I I  +KI+TGDDC+S+GD
Sbjct: 161 DSKNFHINLLGCKNITFEHVTISAPENSPNTDGIHISSSEQIRILNTKISTGDDCVSVGD 220

Query: 509 GSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAAS 568
            +K I I +VTCGPGHGISIGSLG+YT E+ VEGV V  C +I TTNGVRIKTWP SA  
Sbjct: 221 SNKDITIRDVTCGPGHGISIGSLGKYTKEKAVEGVWVTKCKLIKTTNGVRIKTWPDSAVK 280

Query: 569 GTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAI 628
            +A++M F DI M++VSNPILIDQEYCPWNQCNRKIPS IKISKVSFKNIRG+  TPVA+
Sbjct: 281 YSASDMHFEDIEMIDVSNPILIDQEYCPWNQCNRKIPSSIKISKVSFKNIRGTTVTPVAV 340

Query: 629 KLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 677
           KL+CS+ +PC+ V+V DIDL YNG  G ITSQC NV+PIISGKQNP+I
Sbjct: 341 KLVCSKSIPCEAVEVADIDLKYNGNKGSITSQCANVKPIISGKQNPRI 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR_OENOR2.1e-11557.39Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1[more]
PGLR_BRANA8.1e-11253.09Polygalacturonase OS=Brassica napus PE=2 SV=1[more]
PGLR_TOBAC4.5e-11051.21Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1[more]
PGLR2_PLAAC1.9e-10851.70Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1[more]
PGLR_GOSHI3.2e-10852.62Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A5BHX0_VITVI1.7e-16952.40Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_002011 PE=3 SV=1[more]
A0A0A0K3B7_CUCSA8.6e-16177.59Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1[more]
F6HHK3_VITVI1.1e-14254.91Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0057g00380 PE=3 SV=... [more]
F6HHK2_VITVI1.7e-13252.55Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0057g00370 PE=3 SV=... [more]
E3VSV7_CUCPE8.7e-12961.58Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G07820.14.0e-11755.11 Pectin lyase-like superfamily protein[more]
AT5G48140.15.2e-11753.78 Pectin lyase-like superfamily protein[more]
AT3G07840.14.9e-11553.35 Pectin lyase-like superfamily protein[more]
AT3G07830.12.1e-11353.13 Pectin lyase-like superfamily protein[more]
AT3G14040.13.8e-10750.68 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|147835151|emb|CAN65676.1|2.5e-16952.40hypothetical protein VITISV_002011 [Vitis vinifera][more]
gi|659111053|ref|XP_008455553.1|2.7e-16378.45PREDICTED: exopolygalacturonase-like [Cucumis melo][more]
gi|778730103|ref|XP_011659699.1|1.2e-16077.59PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
gi|700188231|gb|KGN43464.1|1.2e-16077.59hypothetical protein Csa_7G037910 [Cucumis sativus][more]
gi|449445367|ref|XP_004140444.1|3.0e-13867.24PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR006626PbH1
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G003100.1CmaCh13G003100.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 340..665
score: 2.8E-99coord: 7..331
score: 1.0
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 184..197
score: -coord: 517..530
scor
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 540..561
score: 2.3coord: 510..530
score: 5600.0coord: 207..228
score: 2.3coord: 177..197
score: 5600.0coord: 127..153
score: 24.0coord: 460..486
score:
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 1..331
score: 2.2E-96coord: 328..674
score: 2.67E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 330..675
score: 8.3E-105coord: 1..329
score: 3.9
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 257..676
score: 1.0E
NoneNo IPR availablePANTHERPTHR31375:SF37PECTIN LYASE-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 257..676
score: 1.0E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh13G003100CmoCh13G003270Cucurbita moschata (Rifu)cmacmoB218
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh13G003100CmaCh18G005950Cucurbita maxima (Rimu)cmacmaB211
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh13G003100Watermelon (97103) v2cmawmbB211
CmaCh13G003100Wax gourdcmawgoB0265
CmaCh13G003100Cucurbita maxima (Rimu)cmacmaB214
CmaCh13G003100Wild cucumber (PI 183967)cmacpiB222
CmaCh13G003100Melon (DHL92) v3.5.1cmameB204
CmaCh13G003100Watermelon (Charleston Gray)cmawcgB183
CmaCh13G003100Watermelon (Charleston Gray)cmawcgB191
CmaCh13G003100Watermelon (97103) v1cmawmB210
CmaCh13G003100Cucurbita pepo (Zucchini)cmacpeB236