CmaCh12G006360 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh12G006360 vs. Swiss-Prot
Match: KEA6_ARATH (K(+) efflux antiporter 6 OS=Arabidopsis thaliana GN=KEA6 PE=2 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 2.1e-233
Identity = 453/579 (78.24%), Postives = 511/579 (88.26%), Query Frame = 1

Query: 13  IFISLLLLSLPSTALALSDAD--HPVLAN-----ATLQSSNVSS-PRNKEGSFADIIDRA 72
           + +S  L    ++  A+SD+D     +AN     A+L +S+ SS  + KEGSFADIIDRA
Sbjct: 22  LLLSFFLCFSVASPRAISDSDLLDETVANSSSSVASLNASSSSSLVKPKEGSFADIIDRA 81

Query: 73  LENEFKENDQNEATDAGGFNNSVAEQQAVLETVARVKS-KKNDTKEEK-FQLQNVFNLDS 132
           LE EF E+DQNE  D G FNNSVA QQAVLETVARVKS KKN+TKEEK FQL +VFNL++
Sbjct: 82  LEKEFNESDQNEVADPGSFNNSVAGQQAVLETVARVKSTKKNETKEEKRFQLHDVFNLNN 141

Query: 133 DNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQP 192
           DN AEDTPTLIDRKDNVFIISN KSKYPVLQLDLRLISDLV+VIVSATCGGIAFACAGQP
Sbjct: 142 DNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLVVVIVSATCGGIAFACAGQP 201

Query: 193 VITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVAVLG 252
           VITGYLLAGSIIGPGG NF+SEMVQVETVAQFGVVFLLFALGLEFST KL+VVR+VAVLG
Sbjct: 202 VITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFALGLEFSTAKLKVVRSVAVLG 261

Query: 253 GLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTI 312
           GL+QI+LFM L GI  S CGG  SEGVFVGAFLSMSSTAVVLKFLMEKNSTN+LHGQVTI
Sbjct: 262 GLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVVLKFLMEKNSTNSLHGQVTI 321

Query: 313 GTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILSRTCIPWFLK 372
           G LILQDCAVGLLFALLPVL GNSGI+ G++S+ KV+V+L++F  VLSILSRTCIPW LK
Sbjct: 322 GILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLLSFLAVLSILSRTCIPWLLK 381

Query: 373 LMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTFEQIE 432
           LM+SLSSQTNELYQLA+VAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA+HT EQIE
Sbjct: 382 LMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAEHTLEQIE 441

Query: 433 PIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKGFGYNSRTSL 492
           PIRN FAALFLASIGML+NV FLW HVDILLA+VILVII+KT +++TVVKGFGYN++T+L
Sbjct: 442 PIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIKTTIVTTVVKGFGYNNKTAL 501

Query: 493 LVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFKLIPAVVHLGV 552
           LVG+SLAQIGEFAFVLLSRASNLHL+EGKLYLLL+GTTALSLVTTPL+FK+IPAVVHLG+
Sbjct: 502 LVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALSLVTTPLVFKMIPAVVHLGI 561

Query: 553 LLRCFSPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
           LL+ FSPD  +E   KG+I+RS+S KQR++L+ +  H S
Sbjct: 562 LLKWFSPDSTIE---KGEIVRSESGKQRMILMSRQSHSS 597

BLAST of CmaCh12G006360 vs. Swiss-Prot
Match: KEA4_ARATH (K(+) efflux antiporter 4 OS=Arabidopsis thaliana GN=KEA4 PE=2 SV=2)

HSP 1 Score: 797.3 bits (2058), Expect = 1.1e-229
Identity = 431/544 (79.23%), Postives = 485/544 (89.15%), Query Frame = 1

Query: 39  NATLQSSNVSSPRNKEGSFADIIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVA 98
           N T+  SN +S + +E SFAD+IDRALE EF +NDQNE  D G FNNSVA+QQAVLETVA
Sbjct: 52  NGTVVESNATSAKPREDSFADMIDRALEKEFPDNDQNEVPDPGSFNNSVADQQAVLETVA 111

Query: 99  RVKSKKNDTKEEKFQLQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRL 158
           RVK KKN+TK +  + ++ FNLD++NG EDTP LIDRKDNVFI+SN KSKYPVLQLDLRL
Sbjct: 112 RVKPKKNETKTK--EEKSFFNLDNENGVEDTPRLIDRKDNVFIMSNPKSKYPVLQLDLRL 171

Query: 159 ISDLVIVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVF 218
           ISDLV+VIVSATCGGIAFACAGQPVITGYLLAGSIIGPGG +FVSEMVQVETVAQFGV+F
Sbjct: 172 ISDLVVVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGLSFVSEMVQVETVAQFGVIF 231

Query: 219 LLFALGLEFSTTKLRVVRAVAVLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMS 278
           LLFALGLEFS  KLRVVRAVA+ GGL+QI LFMCLSGI AS CGG  +EG+FVGAFLSMS
Sbjct: 232 LLFALGLEFSAAKLRVVRAVAIPGGLLQIFLFMCLSGITASLCGGKLTEGIFVGAFLSMS 291

Query: 279 STAVVLKFLMEKNSTNALHGQVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKV 338
           STAVVLKFLME+NS +ALHGQ+T+GTLILQDCAVGLLFALLPVLGG SG+LQGV+SM+K 
Sbjct: 292 STAVVLKFLMERNSISALHGQITVGTLILQDCAVGLLFALLPVLGGTSGVLQGVLSMAKS 351

Query: 339 LVILVTFWVVLSILSRTCIPWFLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLE 398
           L IL+ F   L +LSRT +PWFLKLM SLSSQTNELYQLA+VAFCLLVAWCSDKLGLSLE
Sbjct: 352 LAILIAFLGALFVLSRTWVPWFLKLMTSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLE 411

Query: 399 LGSFAAGVMISTTDLAQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVIL 458
           LGSFAAGVMISTTDLAQHT EQ+EPIRNFFAALFLASIGMLI++ FLWNHVDILLA V+L
Sbjct: 412 LGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIHMHFLWNHVDILLAAVLL 471

Query: 459 VIIVKTIVISTVVKGFGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIG 518
           VI++KT+V++ VVK FGYN++T++LVGMSLAQIGEFAFVLLSRASNLHL+E KLYLLL+G
Sbjct: 472 VIVIKTVVVAIVVKVFGYNNKTAVLVGMSLAQIGEFAFVLLSRASNLHLIESKLYLLLLG 531

Query: 519 TTALSLVTTPLLFKLIPAVVHLGVLLRCFSPDGLVEIGFKGDIIRSDSVKQRVMLIVQGP 578
           TTALSLVTTPLLFKLIPAVVHLGVLLR FSPD   EIGFKG++  S+S K R+ L++QG 
Sbjct: 532 TTALSLVTTPLLFKLIPAVVHLGVLLRWFSPDSSTEIGFKGELYHSESAK-RISLMIQGS 591

Query: 579 -HDS 582
            HDS
Sbjct: 592 LHDS 592

BLAST of CmaCh12G006360 vs. Swiss-Prot
Match: KEA5_ARATH (K(+) efflux antiporter 5 OS=Arabidopsis thaliana GN=KEA5 PE=2 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 2.3e-187
Identity = 363/509 (71.32%), Postives = 426/509 (83.69%), Query Frame = 1

Query: 46  NVSSPRNKEGSFADIIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVARV---KS 105
           N ++P N EGS A + DR LE EF END  E +D   FN+SVA+QQA +ETVA+V   K 
Sbjct: 37  NSTAPGNGEGSIAKMFDRVLEKEFSENDSPEGSDGASFNSSVADQQAEIETVAKVTHEKG 96

Query: 106 KKNDTKEEK----FQLQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRL 165
           K+NDT+E      FQLQ+VF+L++++   D  TLID+K+NVF++SN KSKYP+LQ+DLRL
Sbjct: 97  KRNDTQENNGTRPFQLQDVFSLENEDS--DDMTLIDKKNNVFVMSNKKSKYPILQVDLRL 156

Query: 166 ISDLVIVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVF 225
           ISDLV++IV A  GGI F+C GQPVI GYLLAGSIIGPGG  F+SEMVQVETVAQFGVVF
Sbjct: 157 ISDLVVIIVFAAIGGIVFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVF 216

Query: 226 LLFALGLEFSTTKLRVVRAVAVLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMS 285
           LLFALGLEFS TKL+VV  VAVLGGL+QI+L M L G+ A  CG   SEG+FVGAFLSMS
Sbjct: 217 LLFALGLEFSMTKLKVVGPVAVLGGLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSMS 276

Query: 286 STAVVLKFLMEKNSTNALHGQVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKV 345
           STAVV+KFL+E+NST++LHGQVTIG LI QDC VGLLFALLPVLGGNSG+LQG+ISM K+
Sbjct: 277 STAVVVKFLVERNSTSSLHGQVTIGILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGKL 336

Query: 346 LVILVTFWVVLSILSRTCIPWFLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLE 405
           L+IL  +  V S+L+ + +P FLKLMI LSSQTNELYQLA+VAFCLL AWCSDKLGLSLE
Sbjct: 337 LLILSIYLTVASLLTWSFVPRFLKLMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLE 396

Query: 406 LGSFAAGVMISTTDLAQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVIL 465
           LGSF AGVM+STT+ AQHT EQ+EPIRN FAALFL+SIGMLINV FLWNHVDILLA+VIL
Sbjct: 397 LGSFVAGVMLSTTEFAQHTLEQVEPIRNLFAALFLSSIGMLINVHFLWNHVDILLASVIL 456

Query: 466 VIIVKTIVISTVVKGFGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIG 525
           VI++KT + + VVK F YN R S  VG+ LAQIGEFAFVLLSRASNLH++EGK+YLLL+G
Sbjct: 457 VIVIKTAIAAVVVKAFRYNMRISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLLG 516

Query: 526 TTALSLVTTPLLFKLIPAVVHLGVLLRCF 548
           TTALSLVTTPLLFKLIP+ ++LGVLLR F
Sbjct: 517 TTALSLVTTPLLFKLIPSAMNLGVLLRWF 543

BLAST of CmaCh12G006360 vs. Swiss-Prot
Match: TMCO3_MOUSE (Transmembrane and coiled-coil domain-containing protein 3 OS=Mus musculus GN=Tmco3 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.8e-38
Identity = 152/525 (28.95%), Postives = 270/525 (51.43%), Query Frame = 1

Query: 29  LSDADHPVLANATLQSSNVSSPRNKEGSFADIIDRALENEFKENDQ-NEATDAGGFNNSV 88
           L+   H V A   +Q  N S           ++D  LE+  K  D+  E  +   F+++ 
Sbjct: 157 LAAEKHQVEALKNMQHQNKS---------LSMLDEILEDVRKAADRLEEEIEEHAFDDNK 216

Query: 89  AEQQAVLETVARVKSKKNDTKEEKFQLQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKS 148
           + +    E V RV+ ++  +K      QN+   + ++G      LID ++N +I++  + 
Sbjct: 217 SVKGVNFEAVLRVEEEEASSK------QNMTKREVEDGL-GLSMLIDSQNNQYILTKPRD 276

Query: 149 KYPVLQLDLRLISDLVIV-IVSATCGGIAFACAGQPVITGYLLAGSIIGPGGFNFVSEMV 208
              + + D   I D+V + ++S  CG +  A  G P + GY++ G ++GP G N +  +V
Sbjct: 277 S-TIPRADHHFIKDIVTIGMLSLPCGWLCTAI-GLPTMFGYIICGVLLGPSGLNSIKSIV 336

Query: 209 QVETVAQFGVVFLLFALGLEFSTTKLRVVRAVAVLGGLVQIILFMCLSGIIASSCGGSAS 268
           QVET+ +FGV F LF +GLEFS  KLR V  +++ G     +L +              +
Sbjct: 337 QVETLGEFGVFFTLFLVGLEFSPEKLRKVWRISLQGPCYMTLLMIAFGLWWGHLLRIRPT 396

Query: 269 EGVFVGAFLSMSSTAVVLKFLM-----EKNSTNALHGQVTIGTLILQDCAVGLLFALLPV 328
           + VF+   LS+SST +V +FL+     +K + +  +  V +G L++QD  +GL  A++P 
Sbjct: 397 QSVFISTCLSLSSTPLVSRFLVGSARGDKEAGDIDYSTVLLGMLVMQDVQLGLFIAVMPT 456

Query: 329 L-----GGNSGILQGVIS----MSKVLVILVTFWVVLSILSRTCI-PWFLKLMISLSSQT 388
           L     G +S ++  V+     + ++L  L   +++  ++    I P++ KL +  S   
Sbjct: 457 LIQAGAGASSSVVMEVLRILFLIGQILFSLAAVFLLCLVMKTYLIGPYYRKLHLE-SKGN 516

Query: 389 NELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTD--LAQHTFEQIEPIRNFFA 448
            E+  L   AF  L+   ++ L +S+ELG F AG ++S+    + +     IEPIR+F A
Sbjct: 517 KEILVLGVSAFTFLMLTVTELLDVSMELGCFLAGALVSSQGHMVTEEIMTYIEPIRDFLA 576

Query: 449 ALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKGFGYNSRTSL--LVGMS 508
            +F ASIG+ +   F+   + +L+   + V+I+K ++   V+      S   +  +V   
Sbjct: 577 IIFFASIGLHVFPTFVIYELTVLVFLTLSVVIMKFVLAVLVLSLILPRSSQYIKWIVSAG 636

Query: 509 LAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFK 533
           LAQ+ EF+FVL SRA    ++  ++YLL++  T LSL+  P+L+K
Sbjct: 637 LAQVSEFSFVLGSRARRAGILSREVYLLILSVTTLSLLLAPVLWK 662

BLAST of CmaCh12G006360 vs. Swiss-Prot
Match: TMCO3_HUMAN (Transmembrane and coiled-coil domain-containing protein 3 OS=Homo sapiens GN=TMCO3 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 6.7e-38
Identity = 155/529 (29.30%), Postives = 266/529 (50.28%), Query Frame = 1

Query: 29  LSDADHPVLANATLQSSNVSSPRNKEGSFADIIDRALENEFKENDQ-NEATDAGGFNNSV 88
           L+   H V A   +Q  N S           ++D  LE+  K  D+  E  +   F+++ 
Sbjct: 157 LAAEKHQVEALKNMQHQNQS---------LSMLDEILEDVRKAADRLEEEIEEHAFDDNK 216

Query: 89  AEQQAVLETVARVK-----SKKNDTKEEKFQLQNVFNLDSDNGAEDTPTLIDRKDNVFII 148
           + +    E V RV+     SK+N TK E         ++ D G      LID ++N +I+
Sbjct: 217 SVKGVNFEAVLRVEEEEANSKQNITKRE---------VEDDLGLS---MLIDSQNNQYIL 276

Query: 149 SNFKSKYPVLQLDLRLISDLVIV-IVSATCGGIAFACAGQPVITGYLLAGSIIGPGGFNF 208
           +  +    + + D   I D+V + ++S  CG +  A  G P + GY++ G ++GP G N 
Sbjct: 277 TKPRDS-TIPRADHHFIKDIVTIGMLSLPCGWLCTAI-GLPTMFGYIICGVLLGPSGLNS 336

Query: 209 VSEMVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVAVLGGLVQIILFMCLSGIIASSC 268
           +  +VQVET+ +FGV F LF +GLEFS  KLR V  +++ G     +L +    +     
Sbjct: 337 IKSIVQVETLGEFGVFFTLFLVGLEFSPEKLRKVWKISLQGPCYMTLLMIAFGLLWGHLL 396

Query: 269 GGSASEGVFVGAFLSMSSTAVVLKFLMEK----NSTNALHGQVTIGTLILQDCAVGLLFA 328
               ++ VF+   LS+SST +V +FLM         +  +  V +G L+ QD  +GL  A
Sbjct: 397 RIKPTQSVFISTCLSLSSTPLVSRFLMGSARGDKEGDIDYSTVLLGMLVTQDVQLGLFMA 456

Query: 329 LLPVL--GGNSGILQGVISMSKVLVILVTFWVVLSILSRTCI--------PWFLKLMISL 388
           ++P L   G S     V+ + ++LV++      L+ +   C+        P++ KL +  
Sbjct: 457 VMPTLIQAGASASSSIVVEVLRILVLIGQILFSLAAVFLLCLVIKKYLIGPYYRKLHME- 516

Query: 389 SSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTD--LAQHTFEQIEPIR 448
           S    E+  L   AF  L+   ++ L +S+ELG F AG ++S+    + +     IEPIR
Sbjct: 517 SKGNKEILILGISAFIFLMLTVTELLDVSMELGCFLAGALVSSQGPVVTEEIATSIEPIR 576

Query: 449 NFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKGFGYNSRTSL--L 508
           +F A +F ASIG+ +   F+   + +L+   + V+++K ++ + V+      S   +  +
Sbjct: 577 DFLAIVFFASIGLHVFPTFVAYELTVLVFLTLSVVVMKFLLAALVLSLILPRSSQYIKWI 636

Query: 509 VGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFK 533
           V   LAQ+ EF+FVL SRA    ++  ++YLL++  T LSL+  P+L++
Sbjct: 637 VSAGLAQVSEFSFVLGSRARRAGVISREVYLLILSVTTLSLLLAPVLWR 661

BLAST of CmaCh12G006360 vs. TrEMBL
Match: A0A0A0LNL5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G351730 PE=4 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 4.6e-280
Identity = 537/574 (93.55%), Postives = 549/574 (95.64%), Query Frame = 1

Query: 11  ISIFISLLLLS---LPSTALALSDADHPVLANATLQSSNVSSPRNKEGSFADIIDRALEN 70
           ISIFI L L+S   LPS+AL LSD+D P+LAN TLQS+NVS PRNKEGSFADIIDRALEN
Sbjct: 13  ISIFIFLFLVSPPLLPSSALPLSDSDQPLLANTTLQSNNVSVPRNKEGSFADIIDRALEN 72

Query: 71  EFKENDQNEATDAGGFNNSVAEQQAVLETVARVKSKKNDTKEEKFQLQNVFNLDSDNGAE 130
           EFKENDQNEA D+G FNNSVAEQQA LETVARVKSKKNDTKEEKFQLQNVFNLDSDN AE
Sbjct: 73  EFKENDQNEAIDSGSFNNSVAEQQATLETVARVKSKKNDTKEEKFQLQNVFNLDSDNRAE 132

Query: 131 DTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQPVITGY 190
           DTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQPVITGY
Sbjct: 133 DTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQPVITGY 192

Query: 191 LLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVAVLGGLVQI 250
           LLAGSIIGPGGFNFVSEMVQVETVAQFGV+FLLFALGLEFSTTKLRVVRAVAVLGGL+QI
Sbjct: 193 LLAGSIIGPGGFNFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLRVVRAVAVLGGLLQI 252

Query: 251 ILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLIL 310
            LFMCLSGI ASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLIL
Sbjct: 253 FLFMCLSGITASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLIL 312

Query: 311 QDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILSRTCIPWFLKLMISL 370
           QDCAVGLLFALLPVLGGNSGILQGV+SMSKVLVILV F V LSILSRTCIPW LKLMISL
Sbjct: 313 QDCAVGLLFALLPVLGGNSGILQGVMSMSKVLVILVGFLVALSILSRTCIPWLLKLMISL 372

Query: 371 SSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTFEQIEPIRNF 430
           SSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHT EQIEPIRNF
Sbjct: 373 SSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQIEPIRNF 432

Query: 431 FAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKGFGYNSRTSLLVGMS 490
           FAALFLASIGMLI+VQFLWNHVDILLA VILVIIVKTIVISTVVKGFGYN+RT+LLVGMS
Sbjct: 433 FAALFLASIGMLIHVQFLWNHVDILLAAVILVIIVKTIVISTVVKGFGYNNRTALLVGMS 492

Query: 491 LAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFKLIPAVVHLGVLLRCF 550
           LAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTP LFKLIPAVVHLGVLLR F
Sbjct: 493 LAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPFLFKLIPAVVHLGVLLRWF 552

Query: 551 SPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
           SPD LVEIG KGDIIRSDSVKQRVMLIVQGPHDS
Sbjct: 553 SPDSLVEIGLKGDIIRSDSVKQRVMLIVQGPHDS 586

BLAST of CmaCh12G006360 vs. TrEMBL
Match: M5XN06_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003277mg PE=4 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 3.2e-249
Identity = 486/588 (82.65%), Postives = 522/588 (88.78%), Query Frame = 1

Query: 1   MLKVSSSSD--CISIFISLLLLSLPSTALALSDAD----HPVLANATLQSSNVSSPRNKE 60
           ML+ SSSS     +I I  LLL       +LS A+       L NA    SNVS  + KE
Sbjct: 1   MLRRSSSSSHGLFTILIPTLLLLSSDLLFSLSFAEPLTESDPLLNANATESNVSLSKPKE 60

Query: 61  GSFADIIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVARVKSKKNDTKEEK-FQ 120
           GSFAD+ID+ALENEFKENDQNEATD G FNNSVA QQAVLETVARVKSKKN+TKEEK FQ
Sbjct: 61  GSFADMIDKALENEFKENDQNEATDPGSFNNSVAGQQAVLETVARVKSKKNETKEEKSFQ 120

Query: 121 LQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGG 180
           L +VFNLD+DN  EDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLV+VIVSATCGG
Sbjct: 121 LHDVFNLDNDNRGEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLR 240
           IAFACAGQPVI+GYLLAGS+IGPGGFNFVSEMVQVETVAQFGV+FLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVISGYLLAGSVIGPGGFNFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNST 300
           VVRAVAV GGL+QI LFMCL GI AS CGG  SEG+FVG FLSMSSTAVVLKFLMEKNST
Sbjct: 241 VVRAVAVPGGLLQIFLFMCLCGITASLCGGKVSEGIFVGVFLSMSSTAVVLKFLMEKNST 300

Query: 301 NALHGQVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILS 360
           NALHGQVTIGTLILQDCAVGLLFALLPVLGG SGILQGVISM+K++V L+TF  VLSI S
Sbjct: 301 NALHGQVTIGTLILQDCAVGLLFALLPVLGGTSGILQGVISMAKLMVTLITFLAVLSISS 360

Query: 361 RTCIPWFLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTC+PW LKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPWLLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKG 480
           AQHT EQIEPIRN FAALFLASIGMLI+VQFLWNHVDILLA+VILVII+KTI+I+ VVKG
Sbjct: 421 AQHTLEQIEPIRNLFAALFLASIGMLIHVQFLWNHVDILLASVILVIIIKTIIITMVVKG 480

Query: 481 FGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFKL 540
           FGYN++TS LVG+SLAQIGEFAFVLLSRASNLHLVEGKLYLLL+GTTALSLVTTP LFKL
Sbjct: 481 FGYNNKTSFLVGISLAQIGEFAFVLLSRASNLHLVEGKLYLLLLGTTALSLVTTPFLFKL 540

Query: 541 IPAVVHLGVLLRCFSPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
           IP +VHLGVLLR F PDG VEIGFKGD +R+DS KQRV+L+V+  HDS
Sbjct: 541 IPGLVHLGVLLRWFPPDGAVEIGFKGDNLRTDSGKQRVILMVRESHDS 588

BLAST of CmaCh12G006360 vs. TrEMBL
Match: W9R3F6_9ROSA (K(+) efflux antiporter 6 OS=Morus notabilis GN=L484_019513 PE=4 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 5.5e-249
Identity = 489/591 (82.74%), Postives = 529/591 (89.51%), Query Frame = 1

Query: 1    MLKVSSSSDCISIFISLLLLSL------PST---ALALSDADHPVLANATLQSSNVSSPR 60
            ML+ SSSS    ++I  L L L      PS+   A  L D+D  V  NAT   SNVS  R
Sbjct: 588  MLRGSSSSVRRLVWILALTLLLLCGDLVPSSLARAHPLGDSDLLVQTNAT--ESNVSLSR 647

Query: 61   NKEGSFADIIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVARVKSKKNDTKEEK 120
             KEGSFAD+ID+ALENEFKENDQNEA DAG FNNSVA QQAVLETVARVK KKNDTKEEK
Sbjct: 648  PKEGSFADMIDKALENEFKENDQNEANDAGSFNNSVAGQQAVLETVARVKPKKNDTKEEK 707

Query: 121  -FQLQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSAT 180
             FQ  +VFNLD+DN AEDTPTLIDRKDNVFIISNFKSKYP+LQLDLRLISDLV+VIVSAT
Sbjct: 708  SFQFHDVFNLDNDNRAEDTPTLIDRKDNVFIISNFKSKYPMLQLDLRLISDLVVVIVSAT 767

Query: 181  CGGIAFACAGQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTT 240
            CGGIAFACAGQPVITGYLLAGS+IGPGGF+FVSEMVQVETVAQFGV+FLLFALGLEFSTT
Sbjct: 768  CGGIAFACAGQPVITGYLLAGSVIGPGGFSFVSEMVQVETVAQFGVIFLLFALGLEFSTT 827

Query: 241  KLRVVRAVAVLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEK 300
            KLRVVRAVAVLGGL+QI LFMCL G IAS CGG ASEGVFVG FLSMSSTAVVLKFLM+K
Sbjct: 828  KLRVVRAVAVLGGLLQIFLFMCLCGFIASLCGGKASEGVFVGTFLSMSSTAVVLKFLMDK 887

Query: 301  NSTNALHGQVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLS 360
            NSTNALHGQVTIGTLILQDCAVGLLFALLPVLGG SG+LQG++SM+K+LV+L+TF  VLS
Sbjct: 888  NSTNALHGQVTIGTLILQDCAVGLLFALLPVLGGTSGVLQGLVSMTKLLVVLITFLSVLS 947

Query: 361  ILSRTCIPWFLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMIST 420
            I+SRT +PW LKLM+SLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMI+T
Sbjct: 948  IISRTGLPWLLKLMVSLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMIAT 1007

Query: 421  TDLAQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTV 480
            TDLAQHT EQIEPIRNFFAALFLASIGMLI+VQFLW HVDILLA+VILVIIVKT++ISTV
Sbjct: 1008 TDLAQHTLEQIEPIRNFFAALFLASIGMLIHVQFLWIHVDILLASVILVIIVKTVIISTV 1067

Query: 481  VKGFGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLL 540
            VK F YN++TSLLVGMSLAQIGEFAFVLLSRASNLHLVE KLYLLL+GTTALSLVTTPLL
Sbjct: 1068 VKAFRYNNKTSLLVGMSLAQIGEFAFVLLSRASNLHLVEAKLYLLLLGTTALSLVTTPLL 1127

Query: 541  FKLIPAVVHLGVLLRCFSPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
            FKLIPAVVHLGVLLR F PD  VE+GFKG+ +RSDS KQR++L+VQG HDS
Sbjct: 1128 FKLIPAVVHLGVLLRWFPPDSSVEVGFKGENLRSDSGKQRIILMVQGSHDS 1176

BLAST of CmaCh12G006360 vs. TrEMBL
Match: A0A067KGH2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07679 PE=4 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 5.4e-244
Identity = 465/557 (83.48%), Postives = 507/557 (91.02%), Query Frame = 1

Query: 27  LALSDADHPVLANAT-LQSSNVSSPRNKEGSFADIIDRALENEFKENDQNEATDAGGFNN 86
           L  S++D   L N T + ++++S PR  EGSFA IID AL  EF EN+QN+  D+G FNN
Sbjct: 29  LTESESDRLQLTNDTAVYNASLSKPR--EGSFAKIIDDALRMEFPENEQNDVADSGSFNN 88

Query: 87  SVAEQQAVLETVARVKSKKNDTKEEK-FQLQNVFNLDSDNGAEDTPTLIDRKDNVFIISN 146
           SV+EQQAVLETVARVK KKNDTKEEK F+  +VFNLD+DNGAEDTP LIDRKDNVFIISN
Sbjct: 89  SVSEQQAVLETVARVKPKKNDTKEEKSFKFHDVFNLDNDNGAEDTPMLIDRKDNVFIISN 148

Query: 147 FKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGFNFVSE 206
           FKSKYPVLQLDLRLISDLV+VIVSATCGGIAFACAGQPVITGYLLAGS+IGPGGF+FVSE
Sbjct: 149 FKSKYPVLQLDLRLISDLVVVIVSATCGGIAFACAGQPVITGYLLAGSVIGPGGFSFVSE 208

Query: 207 MVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVAVLGGLVQIILFMCLSGIIASSCGGS 266
           MVQVETVAQFGV+FLLFALGLEFSTTKLRVVRAVAVLGGL+QI+LFM L GI A  CGG 
Sbjct: 209 MVQVETVAQFGVIFLLFALGLEFSTTKLRVVRAVAVLGGLLQIVLFMFLCGITAMLCGGK 268

Query: 267 ASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLILQDCAVGLLFALLPVLGG 326
            S+GVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLILQDCAVGLLFALLPVLGG
Sbjct: 269 PSDGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLILQDCAVGLLFALLPVLGG 328

Query: 327 NSGILQGVISMSKVLVILVTFWVVLSILSRTCIPWFLKLMISLSSQTNELYQLASVAFCL 386
            SG+LQGVIS++++LV+LV F  VLSILS TC+PWFLKLM+SLSSQTNELYQLASVAFCL
Sbjct: 329 TSGVLQGVISVTRLLVVLVAFLAVLSILSHTCVPWFLKLMMSLSSQTNELYQLASVAFCL 388

Query: 387 LVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTFEQIEPIRNFFAALFLASIGMLINVQF 446
           L+AWCSDKLGLSLELGSFAAGVMISTTDLAQHT EQIEPIRNFFAALFLASIGMLINV F
Sbjct: 389 LIAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQIEPIRNFFAALFLASIGMLINVHF 448

Query: 447 LWNHVDILLATVILVIIVKTIVISTVVKGFGYNSRTSLLVGMSLAQIGEFAFVLLSRASN 506
           LWNHVDIL+A+VILVII+KT +I+TVVKGFGYN++T+LLVGMSLAQIGEFAFVLLSRASN
Sbjct: 449 LWNHVDILVASVILVIIIKTTIITTVVKGFGYNNKTALLVGMSLAQIGEFAFVLLSRASN 508

Query: 507 LHLVEGKLYLLLIGTTALSLVTTPLLFKLIPAVVHLGVLLRCFSPDGLVEIGFKGDIIRS 566
           LHLVEGKLYLLL+GTTALSLVTTPLLFKLIPAV HLGVLLR F PD  VE+GFKG+  RS
Sbjct: 509 LHLVEGKLYLLLLGTTALSLVTTPLLFKLIPAVAHLGVLLRWFPPDSSVEVGFKGENFRS 568

Query: 567 DSVKQRVMLIVQGPHDS 582
           DS KQR+ LIVQ PHDS
Sbjct: 569 DSGKQRITLIVQAPHDS 583

BLAST of CmaCh12G006360 vs. TrEMBL
Match: B9INJ5_POPTR (K+ efflux antiporter family protein OS=Populus trichocarpa GN=POPTR_0018s05100g PE=4 SV=2)

HSP 1 Score: 850.1 bits (2195), Expect = 1.6e-243
Identity = 473/574 (82.40%), Postives = 520/574 (90.59%), Query Frame = 1

Query: 10  CISIFISLLLLSLPSTALALSDADH-PVLANATLQSSNVSSPRNKEGSFADIIDRALENE 69
           C  IF+     SL S A  L+++D     AN ++ ++++S PR +  SFADIID+ALE E
Sbjct: 9   CKLIFLLYCFASL-SLADELNESDRLEFTANTSISNTSLSKPR-EGSSFADIIDKALEKE 68

Query: 70  FKENDQNEATDAGGFNNSVAEQQAVLETVARVKSKKNDTKEEK-FQLQNVFNLDSDNGAE 129
           F ENDQNEATDAG FNNSVAEQQAVLETVARVKSKKND+KEEK F+ Q+VFNLD+DNGAE
Sbjct: 69  FTENDQNEATDAGSFNNSVAEQQAVLETVARVKSKKNDSKEEKLFKFQHVFNLDNDNGAE 128

Query: 130 DTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQPVITGY 189
           DTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLV+VIVSATCGGIAFACAGQPVITGY
Sbjct: 129 DTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVVVIVSATCGGIAFACAGQPVITGY 188

Query: 190 LLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVAVLGGLVQI 249
           LLAGSIIGPGGFNFVSEMVQVETVAQFGV+FLLFALGLEFSTTKLRVVRAVAV+GGL++I
Sbjct: 189 LLAGSIIGPGGFNFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLRVVRAVAVVGGLLEI 248

Query: 250 ILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLIL 309
           ILFM L GI A  CGG +SEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLIL
Sbjct: 249 ILFMFLCGITAMLCGGKSSEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLIL 308

Query: 310 QDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILSRTCIPWFLKLMISL 369
           QDCAVGLLFALLPVLGG SG+LQG++SM+KVLV+L+ F  VLSILS T +PWFLKLM+SL
Sbjct: 309 QDCAVGLLFALLPVLGGTSGVLQGLMSMTKVLVVLIAFLAVLSILSCTWVPWFLKLMMSL 368

Query: 370 SSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTFEQIEPIRNF 429
           SSQTNELYQLASVAFCLLVAW SDKLGLSLELGSFAAGVMISTTDLAQHT EQIEPIRNF
Sbjct: 369 SSQTNELYQLASVAFCLLVAWSSDKLGLSLELGSFAAGVMISTTDLAQHTLEQIEPIRNF 428

Query: 430 FAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKGFGYNSRTSLLVGMS 489
           FAALFLASIGMLI+V FLW+HVDILLA+VILVII+KT +I+ VVKGFGYN++TSLLVGMS
Sbjct: 429 FAALFLASIGMLIHVHFLWSHVDILLASVILVIIIKTAIITAVVKGFGYNNKTSLLVGMS 488

Query: 490 LAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFKLIPAVVHLGVLLRCF 549
           LAQIGEFAFVLLSRASNLHLVEGKLYLLL+GTTALSLVTT LLFKLIPAV+HLGVLLR F
Sbjct: 489 LAQIGEFAFVLLSRASNLHLVEGKLYLLLLGTTALSLVTTTLLFKLIPAVMHLGVLLRWF 548

Query: 550 SPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
            PD  VE+G KG+I RSDS KQR+ ++V+ PH+S
Sbjct: 549 PPDSAVEVGSKGEIFRSDSGKQRISVLVEVPHNS 580

BLAST of CmaCh12G006360 vs. TAIR10
Match: AT5G11800.1 (AT5G11800.1 K+ efflux antiporter 6)

HSP 1 Score: 809.7 bits (2090), Expect = 1.2e-234
Identity = 453/579 (78.24%), Postives = 511/579 (88.26%), Query Frame = 1

Query: 13  IFISLLLLSLPSTALALSDAD--HPVLAN-----ATLQSSNVSS-PRNKEGSFADIIDRA 72
           + +S  L    ++  A+SD+D     +AN     A+L +S+ SS  + KEGSFADIIDRA
Sbjct: 22  LLLSFFLCFSVASPRAISDSDLLDETVANSSSSVASLNASSSSSLVKPKEGSFADIIDRA 81

Query: 73  LENEFKENDQNEATDAGGFNNSVAEQQAVLETVARVKS-KKNDTKEEK-FQLQNVFNLDS 132
           LE EF E+DQNE  D G FNNSVA QQAVLETVARVKS KKN+TKEEK FQL +VFNL++
Sbjct: 82  LEKEFNESDQNEVADPGSFNNSVAGQQAVLETVARVKSTKKNETKEEKRFQLHDVFNLNN 141

Query: 133 DNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQP 192
           DN AEDTPTLIDRKDNVFIISN KSKYPVLQLDLRLISDLV+VIVSATCGGIAFACAGQP
Sbjct: 142 DNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLVVVIVSATCGGIAFACAGQP 201

Query: 193 VITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVAVLG 252
           VITGYLLAGSIIGPGG NF+SEMVQVETVAQFGVVFLLFALGLEFST KL+VVR+VAVLG
Sbjct: 202 VITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFALGLEFSTAKLKVVRSVAVLG 261

Query: 253 GLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTI 312
           GL+QI+LFM L GI  S CGG  SEGVFVGAFLSMSSTAVVLKFLMEKNSTN+LHGQVTI
Sbjct: 262 GLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVVLKFLMEKNSTNSLHGQVTI 321

Query: 313 GTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILSRTCIPWFLK 372
           G LILQDCAVGLLFALLPVL GNSGI+ G++S+ KV+V+L++F  VLSILSRTCIPW LK
Sbjct: 322 GILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLLSFLAVLSILSRTCIPWLLK 381

Query: 373 LMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTFEQIE 432
           LM+SLSSQTNELYQLA+VAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLA+HT EQIE
Sbjct: 382 LMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAEHTLEQIE 441

Query: 433 PIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKGFGYNSRTSL 492
           PIRN FAALFLASIGML+NV FLW HVDILLA+VILVII+KT +++TVVKGFGYN++T+L
Sbjct: 442 PIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIKTTIVTTVVKGFGYNNKTAL 501

Query: 493 LVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFKLIPAVVHLGV 552
           LVG+SLAQIGEFAFVLLSRASNLHL+EGKLYLLL+GTTALSLVTTPL+FK+IPAVVHLG+
Sbjct: 502 LVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALSLVTTPLVFKMIPAVVHLGI 561

Query: 553 LLRCFSPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
           LL+ FSPD  +E   KG+I+RS+S KQR++L+ +  H S
Sbjct: 562 LLKWFSPDSTIE---KGEIVRSESGKQRMILMSRQSHSS 597

BLAST of CmaCh12G006360 vs. TAIR10
Match: AT2G19600.1 (AT2G19600.1 K+ efflux antiporter 4)

HSP 1 Score: 797.3 bits (2058), Expect = 6.1e-231
Identity = 431/544 (79.23%), Postives = 485/544 (89.15%), Query Frame = 1

Query: 39  NATLQSSNVSSPRNKEGSFADIIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVA 98
           N T+  SN +S + +E SFAD+IDRALE EF +NDQNE  D G FNNSVA+QQAVLETVA
Sbjct: 52  NGTVVESNATSAKPREDSFADMIDRALEKEFPDNDQNEVPDPGSFNNSVADQQAVLETVA 111

Query: 99  RVKSKKNDTKEEKFQLQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRL 158
           RVK KKN+TK +  + ++ FNLD++NG EDTP LIDRKDNVFI+SN KSKYPVLQLDLRL
Sbjct: 112 RVKPKKNETKTK--EEKSFFNLDNENGVEDTPRLIDRKDNVFIMSNPKSKYPVLQLDLRL 171

Query: 159 ISDLVIVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVF 218
           ISDLV+VIVSATCGGIAFACAGQPVITGYLLAGSIIGPGG +FVSEMVQVETVAQFGV+F
Sbjct: 172 ISDLVVVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGLSFVSEMVQVETVAQFGVIF 231

Query: 219 LLFALGLEFSTTKLRVVRAVAVLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMS 278
           LLFALGLEFS  KLRVVRAVA+ GGL+QI LFMCLSGI AS CGG  +EG+FVGAFLSMS
Sbjct: 232 LLFALGLEFSAAKLRVVRAVAIPGGLLQIFLFMCLSGITASLCGGKLTEGIFVGAFLSMS 291

Query: 279 STAVVLKFLMEKNSTNALHGQVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKV 338
           STAVVLKFLME+NS +ALHGQ+T+GTLILQDCAVGLLFALLPVLGG SG+LQGV+SM+K 
Sbjct: 292 STAVVLKFLMERNSISALHGQITVGTLILQDCAVGLLFALLPVLGGTSGVLQGVLSMAKS 351

Query: 339 LVILVTFWVVLSILSRTCIPWFLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLE 398
           L IL+ F   L +LSRT +PWFLKLM SLSSQTNELYQLA+VAFCLLVAWCSDKLGLSLE
Sbjct: 352 LAILIAFLGALFVLSRTWVPWFLKLMTSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLE 411

Query: 399 LGSFAAGVMISTTDLAQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVIL 458
           LGSFAAGVMISTTDLAQHT EQ+EPIRNFFAALFLASIGMLI++ FLWNHVDILLA V+L
Sbjct: 412 LGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIHMHFLWNHVDILLAAVLL 471

Query: 459 VIIVKTIVISTVVKGFGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIG 518
           VI++KT+V++ VVK FGYN++T++LVGMSLAQIGEFAFVLLSRASNLHL+E KLYLLL+G
Sbjct: 472 VIVIKTVVVAIVVKVFGYNNKTAVLVGMSLAQIGEFAFVLLSRASNLHLIESKLYLLLLG 531

Query: 519 TTALSLVTTPLLFKLIPAVVHLGVLLRCFSPDGLVEIGFKGDIIRSDSVKQRVMLIVQGP 578
           TTALSLVTTPLLFKLIPAVVHLGVLLR FSPD   EIGFKG++  S+S K R+ L++QG 
Sbjct: 532 TTALSLVTTPLLFKLIPAVVHLGVLLRWFSPDSSTEIGFKGELYHSESAK-RISLMIQGS 591

Query: 579 -HDS 582
            HDS
Sbjct: 592 LHDS 592

BLAST of CmaCh12G006360 vs. TAIR10
Match: AT5G51710.1 (AT5G51710.1 K+ efflux antiporter 5)

HSP 1 Score: 656.8 bits (1693), Expect = 1.3e-188
Identity = 363/509 (71.32%), Postives = 426/509 (83.69%), Query Frame = 1

Query: 46  NVSSPRNKEGSFADIIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVARV---KS 105
           N ++P N EGS A + DR LE EF END  E +D   FN+SVA+QQA +ETVA+V   K 
Sbjct: 37  NSTAPGNGEGSIAKMFDRVLEKEFSENDSPEGSDGASFNSSVADQQAEIETVAKVTHEKG 96

Query: 106 KKNDTKEEK----FQLQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRL 165
           K+NDT+E      FQLQ+VF+L++++   D  TLID+K+NVF++SN KSKYP+LQ+DLRL
Sbjct: 97  KRNDTQENNGTRPFQLQDVFSLENEDS--DDMTLIDKKNNVFVMSNKKSKYPILQVDLRL 156

Query: 166 ISDLVIVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVF 225
           ISDLV++IV A  GGI F+C GQPVI GYLLAGSIIGPGG  F+SEMVQVETVAQFGVVF
Sbjct: 157 ISDLVVIIVFAAIGGIVFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVF 216

Query: 226 LLFALGLEFSTTKLRVVRAVAVLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMS 285
           LLFALGLEFS TKL+VV  VAVLGGL+QI+L M L G+ A  CG   SEG+FVGAFLSMS
Sbjct: 217 LLFALGLEFSMTKLKVVGPVAVLGGLLQIVLLMFLCGVTALLCGARLSEGIFVGAFLSMS 276

Query: 286 STAVVLKFLMEKNSTNALHGQVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKV 345
           STAVV+KFL+E+NST++LHGQVTIG LI QDC VGLLFALLPVLGGNSG+LQG+ISM K+
Sbjct: 277 STAVVVKFLVERNSTSSLHGQVTIGILIFQDCVVGLLFALLPVLGGNSGLLQGIISMGKL 336

Query: 346 LVILVTFWVVLSILSRTCIPWFLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLE 405
           L+IL  +  V S+L+ + +P FLKLMI LSSQTNELYQLA+VAFCLL AWCSDKLGLSLE
Sbjct: 337 LLILSIYLTVASLLTWSFVPRFLKLMIQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLE 396

Query: 406 LGSFAAGVMISTTDLAQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVIL 465
           LGSF AGVM+STT+ AQHT EQ+EPIRN FAALFL+SIGMLINV FLWNHVDILLA+VIL
Sbjct: 397 LGSFVAGVMLSTTEFAQHTLEQVEPIRNLFAALFLSSIGMLINVHFLWNHVDILLASVIL 456

Query: 466 VIIVKTIVISTVVKGFGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIG 525
           VI++KT + + VVK F YN R S  VG+ LAQIGEFAFVLLSRASNLH++EGK+YLLL+G
Sbjct: 457 VIVIKTAIAAVVVKAFRYNMRISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYLLLLG 516

Query: 526 TTALSLVTTPLLFKLIPAVVHLGVLLRCF 548
           TTALSLVTTPLLFKLIP+ ++LGVLLR F
Sbjct: 517 TTALSLVTTPLLFKLIPSAMNLGVLLRWF 543

BLAST of CmaCh12G006360 vs. TAIR10
Match: AT4G00630.2 (AT4G00630.2 K+ efflux antiporter 2)

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-33
Identity = 112/366 (30.60%), Postives = 193/366 (52.73%), Query Frame = 1

Query: 180 GQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVA 239
           G PV+ GYL AG +IGP G + +  +   + +A+FGVVFLLF +GLE S  +L  ++   
Sbjct: 592 GSPVL-GYLAAGILIGPYGLSIIRNVHGTKAIAEFGVVFLLFNIGLELSVERLSSMKKYV 651

Query: 240 VLGGLVQIILFMCLSGIIASSCGGSASEG-VFVGAFLSMSSTAVVLKFLMEKNSTNALHG 299
              G  Q+++   + G+I     G A    + +G  L++SSTAVVL+ L E+  + + HG
Sbjct: 652 FGLGSAQVLVTAAVIGLITHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHG 711

Query: 300 QVTIGTLILQDCAVGLLFALLPVLGGNS---GILQGVISMSKVLVILVTFWVVLSILS-- 359
           + T   L+ QD AV +L  L+P++  NS   GI    I+ +  L  +     +  I++  
Sbjct: 712 RATFSVLLFQDLAVVVLLILIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGG 771

Query: 360 ----RTCIPWFLKLMISLSSQ-----TNELYQLASVAFCLLVAWCSDKLGLSLELGSFAA 419
                  I W+ +L+  +  Q       E++   ++   L  +  + + GLS+ LG+F A
Sbjct: 772 RLFYNALIFWYTQLLRPIYKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLA 831

Query: 420 GVMISTTDLAQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKT 479
           G++++ T+ +      I P R     LF  ++GM I+ + L  +  +++ T+ L+++ KT
Sbjct: 832 GLLLAETEFSLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLANFPLIMGTLGLLLVGKT 891

Query: 480 IVISTVVKGFGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSL 531
           I++  + K FG +  +++ VG+ LA  GEFAFV    A N  ++  +L  LL     +S+
Sbjct: 892 ILVVIIGKLFGISIISAVRVGLLLAPGGEFAFVAFGEAVNQGIMTPQLSSLLFLVVGISM 951

BLAST of CmaCh12G006360 vs. TAIR10
Match: AT1G01790.1 (AT1G01790.1 K+ efflux antiporter 1)

HSP 1 Score: 139.0 bits (349), Expect = 9.0e-33
Identity = 109/357 (30.53%), Postives = 188/357 (52.66%), Query Frame = 1

Query: 180 GQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVA 239
           G PV+ GYL AG +IGP G + +  +     +A+FGVVFLLF +GLE S  +L  ++   
Sbjct: 612 GSPVL-GYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFLLFNIGLELSVERLSSMKKYV 671

Query: 240 VLGGLVQIILFMCLSGIIASSCGGSASEG-VFVGAFLSMSSTAVVLKFLMEKNSTNALHG 299
              G  Q+++   + G++A    G A    + +G  L++SSTAVVL+ L E+  + + HG
Sbjct: 672 FGLGSAQVLVTAAVVGLLAHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHG 731

Query: 300 QVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILSRTCIP 359
           + +   L+ QD AV +L  L+P++  NS   +G I    +   L    V  ++     I 
Sbjct: 732 RASFSVLLFQDLAVVVLLILIPLISPNSS--KGGIGFQAIAEALGLAAVKAAVAITAIIA 791

Query: 360 WFLKLMISLSSQ-----TNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 419
               L+  +  Q       E++   ++   L  +  + + GLS+ LG+F AG++++ T+ 
Sbjct: 792 GGRLLLRPIYKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEF 851

Query: 420 AQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKG 479
           +      I P R     LF  ++GM I+ + L ++  +++ T+ L+I+ KT+++  + K 
Sbjct: 852 SLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKL 911

Query: 480 FGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLL 531
           FG +  +++ VG+ LA  GEFAFV    A N  ++  +L  LL     +S+  TP L
Sbjct: 912 FGISIISAIRVGLLLAPGGEFAFVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWL 965

BLAST of CmaCh12G006360 vs. NCBI nr
Match: gi|778670788|ref|XP_011649533.1| (PREDICTED: K(+) efflux antiporter 6 [Cucumis sativus])

HSP 1 Score: 971.5 bits (2510), Expect = 6.7e-280
Identity = 537/574 (93.55%), Postives = 549/574 (95.64%), Query Frame = 1

Query: 11  ISIFISLLLLS---LPSTALALSDADHPVLANATLQSSNVSSPRNKEGSFADIIDRALEN 70
           ISIFI L L+S   LPS+AL LSD+D P+LAN TLQS+NVS PRNKEGSFADIIDRALEN
Sbjct: 13  ISIFIFLFLVSPPLLPSSALPLSDSDQPLLANTTLQSNNVSVPRNKEGSFADIIDRALEN 72

Query: 71  EFKENDQNEATDAGGFNNSVAEQQAVLETVARVKSKKNDTKEEKFQLQNVFNLDSDNGAE 130
           EFKENDQNEA D+G FNNSVAEQQA LETVARVKSKKNDTKEEKFQLQNVFNLDSDN AE
Sbjct: 73  EFKENDQNEAIDSGSFNNSVAEQQATLETVARVKSKKNDTKEEKFQLQNVFNLDSDNRAE 132

Query: 131 DTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQPVITGY 190
           DTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQPVITGY
Sbjct: 133 DTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACAGQPVITGY 192

Query: 191 LLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVAVLGGLVQI 250
           LLAGSIIGPGGFNFVSEMVQVETVAQFGV+FLLFALGLEFSTTKLRVVRAVAVLGGL+QI
Sbjct: 193 LLAGSIIGPGGFNFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLRVVRAVAVLGGLLQI 252

Query: 251 ILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLIL 310
            LFMCLSGI ASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLIL
Sbjct: 253 FLFMCLSGITASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQVTIGTLIL 312

Query: 311 QDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILSRTCIPWFLKLMISL 370
           QDCAVGLLFALLPVLGGNSGILQGV+SMSKVLVILV F V LSILSRTCIPW LKLMISL
Sbjct: 313 QDCAVGLLFALLPVLGGNSGILQGVMSMSKVLVILVGFLVALSILSRTCIPWLLKLMISL 372

Query: 371 SSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTFEQIEPIRNF 430
           SSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHT EQIEPIRNF
Sbjct: 373 SSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQIEPIRNF 432

Query: 431 FAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKGFGYNSRTSLLVGMS 490
           FAALFLASIGMLI+VQFLWNHVDILLA VILVIIVKTIVISTVVKGFGYN+RT+LLVGMS
Sbjct: 433 FAALFLASIGMLIHVQFLWNHVDILLAAVILVIIVKTIVISTVVKGFGYNNRTALLVGMS 492

Query: 491 LAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFKLIPAVVHLGVLLRCF 550
           LAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTP LFKLIPAVVHLGVLLR F
Sbjct: 493 LAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPFLFKLIPAVVHLGVLLRWF 552

Query: 551 SPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
           SPD LVEIG KGDIIRSDSVKQRVMLIVQGPHDS
Sbjct: 553 SPDSLVEIGLKGDIIRSDSVKQRVMLIVQGPHDS 586

BLAST of CmaCh12G006360 vs. NCBI nr
Match: gi|659087468|ref|XP_008444469.1| (PREDICTED: K(+) efflux antiporter 6 isoform X1 [Cucumis melo])

HSP 1 Score: 963.0 bits (2488), Expect = 2.4e-277
Identity = 535/582 (91.92%), Postives = 550/582 (94.50%), Query Frame = 1

Query: 1   MLKVSSSSDCISIFISLLLLSLPST-ALALSDADHPVLANATLQSSNVSSPRNKEGSFAD 60
           ML + SSS  ISI I + L S P   + +LSD+D P+LANATL S+NVS PRNKEGSFAD
Sbjct: 1   MLNLFSSSHRISISIFIFLFSAPLLPSSSLSDSDQPLLANATLLSNNVSVPRNKEGSFAD 60

Query: 61  IIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVARVKSKKNDTKEEKFQLQNVFN 120
           IIDRALENEFKENDQNEA DAG FNNSVAEQQA LETVARVKSKKNDTKEEKFQLQNVFN
Sbjct: 61  IIDRALENEFKENDQNEAIDAGSFNNSVAEQQATLETVARVKSKKNDTKEEKFQLQNVFN 120

Query: 121 LDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACA 180
           LDSDN AEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACA
Sbjct: 121 LDSDNRAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGGIAFACA 180

Query: 181 GQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLRVVRAVA 240
           GQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGV+FLLFALGLEFSTTKLRVVRAVA
Sbjct: 181 GQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLRVVRAVA 240

Query: 241 VLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQ 300
           VLGGL+QI LFMCLSGI ASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQ
Sbjct: 241 VLGGLLQIFLFMCLSGITASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNSTNALHGQ 300

Query: 301 VTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILSRTCIPW 360
           VTIGTLILQDCAVGLLFALLPVLGGNSGILQGV+SMSKVLVILV F + LSILSRTCIPW
Sbjct: 301 VTIGTLILQDCAVGLLFALLPVLGGNSGILQGVMSMSKVLVILVGFLIALSILSRTCIPW 360

Query: 361 FLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTFE 420
            LKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHT E
Sbjct: 361 LLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLE 420

Query: 421 QIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKGFGYNSR 480
           QIEPIRNFFAALFLASIGMLI+VQFLWNHVDILLA VI+VIIVKTIVISTVVKGFGYN+R
Sbjct: 421 QIEPIRNFFAALFLASIGMLIHVQFLWNHVDILLAAVIIVIIVKTIVISTVVKGFGYNNR 480

Query: 481 TSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFKLIPAVVH 540
           T+LLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTP LFKLIPAVVH
Sbjct: 481 TALLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPFLFKLIPAVVH 540

Query: 541 LGVLLRCFSPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
           LGVLLR FSPD LVEIGFKGDIIRSDSVKQRVMLIVQG HDS
Sbjct: 541 LGVLLRWFSPDSLVEIGFKGDIIRSDSVKQRVMLIVQGSHDS 582

BLAST of CmaCh12G006360 vs. NCBI nr
Match: gi|645227324|ref|XP_008220461.1| (PREDICTED: K(+) efflux antiporter 6 isoform X2 [Prunus mume])

HSP 1 Score: 869.8 bits (2246), Expect = 2.7e-249
Identity = 487/588 (82.82%), Postives = 521/588 (88.61%), Query Frame = 1

Query: 1   MLKVSSSSD--CISIFISLLLLSLPSTALALSDAD----HPVLANATLQSSNVSSPRNKE 60
           ML+ SSSS     SI I  LL        +LS A+       L NA    SNVS  + KE
Sbjct: 1   MLRRSSSSSHGLFSILIPTLLFLSSDLLFSLSFAEPLTESDPLLNANATESNVSLSKPKE 60

Query: 61  GSFADIIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVARVKSKKNDTKEEK-FQ 120
           GSFAD+ID+ALENEFKENDQNEATD G FNNSVA QQAVLETVARVKSKKN+TKEEK FQ
Sbjct: 61  GSFADMIDKALENEFKENDQNEATDPGSFNNSVAGQQAVLETVARVKSKKNETKEEKSFQ 120

Query: 121 LQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGG 180
           L +VFNLD+DN  EDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLV+VIVSATCGG
Sbjct: 121 LHDVFNLDNDNRGEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLR 240
           IAFACAGQPVI+GYLLAGS+IGPGGFNFVSEMVQVETVAQFGV+FLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVISGYLLAGSVIGPGGFNFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNST 300
           VVRAVAV GGL+QI LFMCL GI AS CGG  SEG+FVG FLSMSSTAVVLKFLMEKNST
Sbjct: 241 VVRAVAVPGGLLQIFLFMCLCGITASLCGGKVSEGIFVGVFLSMSSTAVVLKFLMEKNST 300

Query: 301 NALHGQVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILS 360
           NALHGQVTIGTLILQDCAVGLLFALLPVLGG SGILQGVISM+K++V L+TF  VLSI S
Sbjct: 301 NALHGQVTIGTLILQDCAVGLLFALLPVLGGTSGILQGVISMAKLMVTLITFLAVLSISS 360

Query: 361 RTCIPWFLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTC+PW LKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPWLLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKG 480
           AQHT EQIEPIRN FAALFLASIGMLI+VQFLWNHVDILLA+VILVII+KTI+I+ VVKG
Sbjct: 421 AQHTLEQIEPIRNLFAALFLASIGMLIHVQFLWNHVDILLASVILVIIIKTIIITMVVKG 480

Query: 481 FGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFKL 540
           FGYN++TS LVG+SLAQIGEFAFVLLSRASNLHLVEGKLYLLL+GTTALSLVTTP LFKL
Sbjct: 481 FGYNNKTSFLVGISLAQIGEFAFVLLSRASNLHLVEGKLYLLLLGTTALSLVTTPFLFKL 540

Query: 541 IPAVVHLGVLLRCFSPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
           IP +VHLGVLLR F PDG VEIGFKGD +RSDS KQRV+L+V+  HDS
Sbjct: 541 IPGLVHLGVLLRWFPPDGAVEIGFKGDNLRSDSGKQRVILMVRESHDS 588

BLAST of CmaCh12G006360 vs. NCBI nr
Match: gi|596286846|ref|XP_007225682.1| (hypothetical protein PRUPE_ppa003277mg [Prunus persica])

HSP 1 Score: 869.0 bits (2244), Expect = 4.7e-249
Identity = 486/588 (82.65%), Postives = 522/588 (88.78%), Query Frame = 1

Query: 1   MLKVSSSSD--CISIFISLLLLSLPSTALALSDAD----HPVLANATLQSSNVSSPRNKE 60
           ML+ SSSS     +I I  LLL       +LS A+       L NA    SNVS  + KE
Sbjct: 1   MLRRSSSSSHGLFTILIPTLLLLSSDLLFSLSFAEPLTESDPLLNANATESNVSLSKPKE 60

Query: 61  GSFADIIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVARVKSKKNDTKEEK-FQ 120
           GSFAD+ID+ALENEFKENDQNEATD G FNNSVA QQAVLETVARVKSKKN+TKEEK FQ
Sbjct: 61  GSFADMIDKALENEFKENDQNEATDPGSFNNSVAGQQAVLETVARVKSKKNETKEEKSFQ 120

Query: 121 LQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATCGG 180
           L +VFNLD+DN  EDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLV+VIVSATCGG
Sbjct: 121 LHDVFNLDNDNRGEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVVVIVSATCGG 180

Query: 181 IAFACAGQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTKLR 240
           IAFACAGQPVI+GYLLAGS+IGPGGFNFVSEMVQVETVAQFGV+FLLFALGLEFSTTKLR
Sbjct: 181 IAFACAGQPVISGYLLAGSVIGPGGFNFVSEMVQVETVAQFGVIFLLFALGLEFSTTKLR 240

Query: 241 VVRAVAVLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKNST 300
           VVRAVAV GGL+QI LFMCL GI AS CGG  SEG+FVG FLSMSSTAVVLKFLMEKNST
Sbjct: 241 VVRAVAVPGGLLQIFLFMCLCGITASLCGGKVSEGIFVGVFLSMSSTAVVLKFLMEKNST 300

Query: 301 NALHGQVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSILS 360
           NALHGQVTIGTLILQDCAVGLLFALLPVLGG SGILQGVISM+K++V L+TF  VLSI S
Sbjct: 301 NALHGQVTIGTLILQDCAVGLLFALLPVLGGTSGILQGVISMAKLMVTLITFLAVLSISS 360

Query: 361 RTCIPWFLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420
           RTC+PW LKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL
Sbjct: 361 RTCVPWLLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTTDL 420

Query: 421 AQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVVKG 480
           AQHT EQIEPIRN FAALFLASIGMLI+VQFLWNHVDILLA+VILVII+KTI+I+ VVKG
Sbjct: 421 AQHTLEQIEPIRNLFAALFLASIGMLIHVQFLWNHVDILLASVILVIIIKTIIITMVVKG 480

Query: 481 FGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLFKL 540
           FGYN++TS LVG+SLAQIGEFAFVLLSRASNLHLVEGKLYLLL+GTTALSLVTTP LFKL
Sbjct: 481 FGYNNKTSFLVGISLAQIGEFAFVLLSRASNLHLVEGKLYLLLLGTTALSLVTTPFLFKL 540

Query: 541 IPAVVHLGVLLRCFSPDGLVEIGFKGDIIRSDSVKQRVMLIVQGPHDS 582
           IP +VHLGVLLR F PDG VEIGFKGD +R+DS KQRV+L+V+  HDS
Sbjct: 541 IPGLVHLGVLLRWFPPDGAVEIGFKGDNLRTDSGKQRVILMVRESHDS 588

BLAST of CmaCh12G006360 vs. NCBI nr
Match: gi|1009134610|ref|XP_015884540.1| (PREDICTED: K(+) efflux antiporter 6 [Ziziphus jujuba])

HSP 1 Score: 868.6 bits (2243), Expect = 6.1e-249
Identity = 486/593 (81.96%), Postives = 539/593 (90.89%), Query Frame = 1

Query: 1   MLKVSSSSDCISIFI---SLLLLSL---PSTALA--LSDADHPVLANATLQSSNVSSPRN 60
           ML+  SSS+   +FI   +L L  L   PS +LA  L+D+D  +L+NA+   SNVS+ + 
Sbjct: 1   MLRRLSSSELSLLFILAPTLFLFCLDWLPSLSLAQPLNDSDRLLLSNAS--ESNVSTYKP 60

Query: 61  KEGSFADIIDRALENEFKENDQNEATDAGGFNNSVAEQQAVLETVARVKSKKNDTKEEK- 120
           KEGSFAD+ID+ALENEFK+N+QNEA DAG FNNSVA QQAVLETVARVKSKKN+TKEEK 
Sbjct: 61  KEGSFADMIDKALENEFKDNEQNEAADAGSFNNSVAGQQAVLETVARVKSKKNETKEEKS 120

Query: 121 FQLQNVFNLDSDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVIVIVSATC 180
           FQL NVFNLD+DNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLV+VIVSATC
Sbjct: 121 FQLHNVFNLDNDNGAEDTPTLIDRKDNVFIISNFKSKYPVLQLDLRLISDLVVVIVSATC 180

Query: 181 GGIAFACAGQPVITGYLLAGSIIGPGGFNFVSEMVQVETVAQFGVVFLLFALGLEFSTTK 240
           GGIAFACAGQPVITGYLLAGS+IGPGGF+FVSEMVQVETVAQFGV+FLLFALGLEFSTTK
Sbjct: 181 GGIAFACAGQPVITGYLLAGSVIGPGGFSFVSEMVQVETVAQFGVIFLLFALGLEFSTTK 240

Query: 241 LRVVRAVAVLGGLVQIILFMCLSGIIASSCGGSASEGVFVGAFLSMSSTAVVLKFLMEKN 300
           LRVVRAVAVLGGL+QI LFMCLSGI AS CGG ASEGVFVG FLSMSSTAVVLKFLMEKN
Sbjct: 241 LRVVRAVAVLGGLLQIFLFMCLSGITASLCGGKASEGVFVGTFLSMSSTAVVLKFLMEKN 300

Query: 301 STNALHGQVTIGTLILQDCAVGLLFALLPVLGGNSGILQGVISMSKVLVILVTFWVVLSI 360
           STNALHGQVTIGTLILQDCAVGLLFALLPVLGG SG+LQG++SM+K+ ++L+ F  VLSI
Sbjct: 301 STNALHGQVTIGTLILQDCAVGLLFALLPVLGGTSGVLQGLMSMTKLTMVLIAFLAVLSI 360

Query: 361 LSRTCIPWFLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMISTT 420
           LSRTC+PW LKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMI+TT
Sbjct: 361 LSRTCVPWLLKLMISLSSQTNELYQLASVAFCLLVAWCSDKLGLSLELGSFAAGVMIATT 420

Query: 421 DLAQHTFEQIEPIRNFFAALFLASIGMLINVQFLWNHVDILLATVILVIIVKTIVISTVV 480
           DLAQHT EQIEPIRNFFAALFLASIGMLI+VQFLWNHVD+LLA+VILVI +KTI+++ VV
Sbjct: 421 DLAQHTLEQIEPIRNFFAALFLASIGMLIHVQFLWNHVDVLLASVILVITIKTIIVTAVV 480

Query: 481 KGFGYNSRTSLLVGMSLAQIGEFAFVLLSRASNLHLVEGKLYLLLIGTTALSLVTTPLLF 540
           KGFGYN++TSLLVG+SLAQIGEFAFVLLSRASNL LVEGKLYLLL+GTTALSLVTTPL+F
Sbjct: 481 KGFGYNNKTSLLVGLSLAQIGEFAFVLLSRASNLKLVEGKLYLLLLGTTALSLVTTPLVF 540

Query: 541 KLIPAVVHLGVLLRCFSPDGLVEIGFKG--DIIRSDSVK-QRVMLIVQGPHDS 582
           KLIPAVVHLGVLLR F  D  +E+GFKG  D +R++S K QRV+++VQGPHDS
Sbjct: 541 KLIPAVVHLGVLLRWFPIDSSIEVGFKGDKDNLRTESGKQQRVIVMVQGPHDS 591