CmaCh12G005440 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh12G005440
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Calcium-binding EF-hand family protein, putative
Location	Cma_Chr12 : 2834560 .. 2834844 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCCATTAAGATCCCAATTGCGGATGTCGAGGGAGGAGATGAAGGAGATCTTTAGAGAGCACGACATCGACGGAGATGGCGTCCTTAGCATCAGTGAGCTCACCCACGCCTTCGGCTTCTTAGGCAATATTCTTCCATACTACAAAGCTCACTATGGTCTCACTTATGCTGACTATGATTGTAATGGCTTTATCACTGAAGATGAGCTTGATAGGCTTGTTGACTATGCCATGAGGTTTCACTCCCCCAAGTGTTGTAACCATGGTAGATCCATCGTGTAA

mRNA sequence

ATGGCGCCATTAAGATCCCAATTGCGGATGTCGAGGGAGGAGATGAAGGAGATCTTTAGAGAGCACGACATCGACGGAGATGGCGTCCTTAGCATCAGTGAGCTCACCCACGCCTTCGGCTTCTTAGGCAATATTCTTCCATACTACAAAGCTCACTATGGTCTCACTTATGCTGACTATGATTGTAATGGCTTTATCACTGAAGATGAGCTTGATAGGCTTGTTGACTATGCCATGAGGTTTCACTCCCCCAAGTGTTGTAACCATGGTAGATCCATCGTGTAA

Coding sequence (CDS)

ATGGCGCCATTAAGATCCCAATTGCGGATGTCGAGGGAGGAGATGAAGGAGATCTTTAGAGAGCACGACATCGACGGAGATGGCGTCCTTAGCATCAGTGAGCTCACCCACGCCTTCGGCTTCTTAGGCAATATTCTTCCATACTACAAAGCTCACTATGGTCTCACTTATGCTGACTATGATTGTAATGGCTTTATCACTGAAGATGAGCTTGATAGGCTTGTTGACTATGCCATGAGGTTTCACTCCCCCAAGTGTTGTAACCATGGTAGATCCATCGTGTAA

Protein sequence

MAPLRSQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADYDCNGFITEDELDRLVDYAMRFHSPKCCNHGRSIV

Homology

[Close]

BLAST of CmaCh12G005440 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.3e-22
Identity = 52/87 (59.77%), Postives = 71/87 (81.61%), Query Frame = 1


Query: 1  MAPLRSQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADY 60
          M+   ++L++SRE++KEIFREHDIDGDG LS++E+  AFG  G+ILP+YKAHYG+ +AD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DCNGFITEDELDRLVDYAMRF-HSPKC 87
          + +GFI E+ELD+LVDYA +F H  KC
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of CmaCh12G005440 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.3e-22
Identity = 52/76 (68.42%), Postives = 62/76 (81.58%), Query Frame = 1


Query: 6  SQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADYDCNGF 65
          S+   SREEMKEIFREHDIDGDG LSISEL  A GFLG+ +P+YKAHYG+ Y D + +G 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 ITEDELDRLVDYAMRF 82
          I+EDELD+L+DYA RF
Sbjct: 67 ISEDELDKLIDYAERF 82

[Close]

BLAST of CmaCh12G005440 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 2.8e-17
Identity = 42/80 (52.50%), Postives = 62/80 (77.50%), Query Frame = 1


Query: 6   SQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADYDCNGF 65
           S ++ SRE++KEIF+ HD D DG L++ E+T AF FLG ILP YKA++G+ YAD + +G 
Sbjct: 97  SNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGL 156

Query: 66  ITEDELDRLVDYAMRFHSPK 86
           I++DEL++LVDYA +F + +
Sbjct: 157 ISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmaCh12G005440 vs. TrEMBL
Match: A0A0A0LNG3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357860 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.2e-15
Identity = 45/110 (40.91%), Postives = 70/110 (63.64%), Query Frame = 1


Query: 1   MAPLRSQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADY 60
           MA  RSQL+++R+E++EI  EHD+DGDG L+  E+  A   +G+++ + KAHYG+++AD 
Sbjct: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61  DCNGFIT--EDELDRLVDYAMRFHSP--------------KCCNHGRSIV 95
           D +G +   E E++ L+DY MRF +P              K C+ G+SIV
Sbjct: 61  DGDGKVDLGEAEMENLIDYVMRFQTPRAPKTPKVPKVPAQKPCHDGKSIV 110

[Close]

BLAST of CmaCh12G005440 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.2e-15
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 1


Query: 5  RSQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADYDCNG 64
          +S  ++ +EEMKE+FREHD +GDG LSI EL+ AFG  G   P Y+A +GL  AD D +G
Sbjct: 10 KSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDG 69

Query: 65 FITEDELDRLVDYAMR 81
          FI+E ELD++VDYA++
Sbjct: 70 FISEQELDKVVDYAIK 85

[Close]

BLAST of CmaCh12G005440 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 113.6 bits (283), Expect = 1.9e-22
Identity = 52/87 (59.77%), Postives = 71/87 (81.61%), Query Frame = 1


Query: 1  MAPLRSQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADY 60
          M+   ++L++SRE++KEIFREHDIDGDG LS++E+  AFG  G+ILP+YKAHYG+ +AD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DCNGFITEDELDRLVDYAMRF-HSPKC 87
          + +GFI E+ELD+LVDYA +F H  KC
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of CmaCh12G005440 vs. NCBI nr
Match: gi|700207406|gb|KGN62525.1| (hypothetical protein Csa_2G359940 [Cucumis sativus])

HSP 1 Score: 113.6 bits (283), Expect = 1.9e-22
Identity = 52/76 (68.42%), Postives = 62/76 (81.58%), Query Frame = 1


Query: 6  SQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADYDCNGF 65
          S+   SREEMKEIFREHDIDGDG LSISEL  A GFLG+ +P+YKAHYG+ Y D + +G 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 ITEDELDRLVDYAMRF 82
          I+EDELD+L+DYA RF
Sbjct: 67 ISEDELDKLIDYAERF 82

[Close]

BLAST of CmaCh12G005440 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 112.8 bits (281), Expect = 3.2e-22
Identity = 51/82 (62.20%), Postives = 69/82 (84.15%), Query Frame = 1


Query: 6   SQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADYDCNGF 65
           ++L++SRE++KEIFREHDIDGDG LS++E+  AFG  G+ILP+YKAHYG+ +AD + +GF
Sbjct: 87  TRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGF 146

Query: 66  ITEDELDRLVDYAMRF-HSPKC 87
           I E+ELD+LVDYA +F H  KC
Sbjct: 147 IEEEELDKLVDYAQKFQHKRKC 168

[Close]

BLAST of CmaCh12G005440 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 95.9 bits (237), Expect = 4.0e-17
Identity = 42/80 (52.50%), Postives = 62/80 (77.50%), Query Frame = 1


Query: 6   SQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADYDCNGF 65
           S ++ SRE++KEIF+ HD D DG L++ E+T AF FLG ILP YKA++G+ YAD + +G 
Sbjct: 97  SNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGL 156

Query: 66  ITEDELDRLVDYAMRFHSPK 86
           I++DEL++LVDYA +F + +
Sbjct: 157 ISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmaCh12G005440 vs. NCBI nr
Match: gi|700207395|gb|KGN62514.1| (hypothetical protein Csa_2G357860 [Cucumis sativus])

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-15
Identity = 45/110 (40.91%), Postives = 70/110 (63.64%), Query Frame = 1


Query: 1   MAPLRSQLRMSREEMKEIFREHDIDGDGVLSISELTHAFGFLGNILPYYKAHYGLTYADY 60
           MA  RSQL+++R+E++EI  EHD+DGDG L+  E+  A   +G+++ + KAHYG+++AD 
Sbjct: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61  DCNGFIT--EDELDRLVDYAMRFHSP--------------KCCNHGRSIV 95
           D +G +   E E++ L+DY MRF +P              K C+ G+SIV
Sbjct: 61  DGDGKVDLGEAEMENLIDYVMRFQTPRAPKTPKVPKVPAQKPCHDGKSIV 110

The following BLAST results are available for this feature:

BLAST of CmaCh12G005440 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CmaCh12G005440 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC1_CUCSA	1.3e-22	59.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	1.3e-22	68.42	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	2.8e-17	52.50	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0LNG3_CUCSA	1.2e-15	40.91	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357860 PE=4 SV=1	[more]
A0A0A0LKW4_CUCSA	1.2e-15	55.26	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1	[more]

BLAST of CmaCh12G005440 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmaCh12G005440 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207397\|gb\|KGN62516.1\|	1.9e-22	59.77	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]
gi\|700207406\|gb\|KGN62525.1\|	1.9e-22	68.42	hypothetical protein Csa_2G359940 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	3.2e-22	62.20	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700194513\|gb\|KGN49690.1\|	4.0e-17	52.50	hypothetical protein Csa_5G068170 [Cucumis sativus]	[more]
gi\|700207395\|gb\|KGN62514.1\|	1.7e-15	40.91	hypothetical protein Csa_2G357860 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G005440.1	CmaCh12G005440.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 14..39 score: 1.
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 58..75 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 59..81 score: 6.256coord: 10..45 score: 13
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 10..75 score: 2.7
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 6..73 score: 6.27
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35 score: -coord: 59..71 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 6..79 score: 8.8
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 6..79 score: 8.8

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh12G005440	Cucurbita maxima (Rimu)	cmacmaB199
CmaCh12G005440	Cucurbita maxima (Rimu)	cmacmaB182
CmaCh12G005440	Cucumber (Gy14) v1	cgycmaB0638
CmaCh12G005440	Cucurbita moschata (Rifu)	cmacmoB176
CmaCh12G005440	Cucurbita moschata (Rifu)	cmacmoB199
CmaCh12G005440	Wild cucumber (PI 183967)	cmacpiB175
CmaCh12G005440	Cucumber (Chinese Long) v2	cmacuB174
CmaCh12G005440	Cucumber (Chinese Long) v2	cmacuB186
CmaCh12G005440	Melon (DHL92) v3.5.1	cmameB156
CmaCh12G005440	Melon (DHL92) v3.5.1	cmameB182
CmaCh12G005440	Watermelon (Charleston Gray)	cmawcgB160
CmaCh12G005440	Watermelon (Charleston Gray)	cmawcgB169
CmaCh12G005440	Watermelon (97103) v1	cmawmB148
CmaCh12G005440	Watermelon (97103) v1	cmawmB159
CmaCh12G005440	Cucurbita pepo (Zucchini)	cmacpeB179
CmaCh12G005440	Cucurbita pepo (Zucchini)	cmacpeB203
CmaCh12G005440	Bottle gourd (USVL1VR-Ls)	cmalsiB184
CmaCh12G005440	Cucumber (Gy14) v2	cgybcmaB179
CmaCh12G005440	Cucumber (Gy14) v2	cgybcmaB479
CmaCh12G005440	Melon (DHL92) v3.6.1	cmamedB170
CmaCh12G005440	Melon (DHL92) v3.6.1	cmamedB198
CmaCh12G005440	Silver-seed gourd	carcmaB0100
CmaCh12G005440	Silver-seed gourd	carcmaB0249
CmaCh12G005440	Silver-seed gourd	carcmaB1067
CmaCh12G005440	Cucumber (Chinese Long) v3	cmacucB0203
CmaCh12G005440	Cucumber (Chinese Long) v3	cmacucB0215
CmaCh12G005440	Watermelon (97103) v2	cmawmbB194
CmaCh12G005440	Wax gourd	cmawgoB0194