CmaCh12G005420 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh12G005420
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Unknown protein
Location	Cma_Chr12 : 2820302 .. 2820568 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAAGTGTATCAAACAGGATGCGACTCTCTCGAGAGGAGATCAAGGAGATTTTCAGAGAGCATGACATCGACGGTGATGGTCAGCTTTCCATGCAGGAGGTGATGAAGGCCTTTGGGTTCCTCGGCTGCATGCTTCCTTACCACAAAGCTCACTATGGCATGGCTCTTGCGGATGCTGATGGCGATGGCTTGATCGACGAGGATGAGCTCGAAAGGCTCGTGGACTATGCCGAGAGGTTCCAGAACAGGAAGCGCTGTGGTTAA

mRNA sequence

ATGTCAAGTGTATCAAACAGGATGCGACTCTCTCGAGAGGAGATCAAGGAGATTTTCAGAGAGCATGACATCGACGGTGATGGTCAGCTTTCCATGCAGGAGGTGATGAAGGCCTTTGGGTTCCTCGGCTGCATGCTTCCTTACCACAAAGCTCACTATGGCATGGCTCTTGCGGATGCTGATGGCGATGGCTTGATCGACGAGGATGAGCTCGAAAGGCTCGTGGACTATGCCGAGAGGTTCCAGAACAGGAAGCGCTGTGGTTAA

Coding sequence (CDS)

ATGTCAAGTGTATCAAACAGGATGCGACTCTCTCGAGAGGAGATCAAGGAGATTTTCAGAGAGCATGACATCGACGGTGATGGTCAGCTTTCCATGCAGGAGGTGATGAAGGCCTTTGGGTTCCTCGGCTGCATGCTTCCTTACCACAAAGCTCACTATGGCATGGCTCTTGCGGATGCTGATGGCGATGGCTTGATCGACGAGGATGAGCTCGAAAGGCTCGTGGACTATGCCGAGAGGTTCCAGAACAGGAAGCGCTGTGGTTAA

Protein sequence

MSSVSNRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADADGDGLIDEDELERLVDYAERFQNRKRCG

Homology

[Close]

BLAST of CmaCh12G005420 vs. Swiss-Prot
Match: CML23_ORYSJ (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica GN=CML23 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.9e-06
Identity = 29/71 (40.85%), Postives = 41/71 (57.75%), Query Frame = 1


Query: 10 LSREEIKEIFREHDIDGDGQLSMQEVMKAF-GFLGCMLPYHKAHYGMALADADGDGLIDE 69
          ++ +E + +F   D DGDG++S  E+       LG  +P  +    MALAD DGDGL+DE
Sbjct: 2  VASDEFRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDE 61

Query: 70 DELERLVDYAE 80
          +E  RLV   E
Sbjct: 62 EEFVRLVTEME 72

[Close]

BLAST of CmaCh12G005420 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 4.7e-27
Identity = 56/87 (64.37%), Postives = 77/87 (88.51%), Query Frame = 1


Query: 1  MSSVSNRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADA 60
          MS  + R++LSRE+IKEIFREHDIDGDG+LSM E++KAFG  G +LP++KAHYGMA AD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGLIDEDELERLVDYAERFQNRKRC 88
          +GDG I+E+EL++LVDYA++FQ++++C
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of CmaCh12G005420 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 9.9e-25
Identity = 54/81 (66.67%), Postives = 68/81 (83.95%), Query Frame = 1


Query: 6  NRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADADGDGL 65
          ++   SREE+KEIFREHDIDGDG LS+ E++KA GFLG  +P++KAHYGMA  D +GDGL
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 IDEDELERLVDYAERFQNRKR 87
          I EDEL++L+DYAERFQ R+R
Sbjct: 67 ISEDELDKLIDYAERFQKRRR 87

[Close]

BLAST of CmaCh12G005420 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 8.1e-19
Identity = 47/81 (58.02%), Postives = 65/81 (80.25%), Query Frame = 1


Query: 5   SNRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADADGDG 64
           ++ M+ SRE+IKEIF+ HD D DG L+++EV KAF FLG +LP +KA++GM  ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  LIDEDELERLVDYAERFQNRK 86
           LI +DELE+LVDYA +F N++
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmaCh12G005420 vs. TrEMBL
Match: A0A0A0LP46_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358880 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.2e-17
Identity = 48/79 (60.76%), Postives = 57/79 (72.15%), Query Frame = 1


Query: 8   MRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADADGDGLID 67
           M+L+REEIKEIF   DIDGDG LS  EV++AFG +G   P  KAHY MA AD DGDG I 
Sbjct: 57  MKLTREEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGDGRIS 116

Query: 68  EDELERLVDYAERFQNRKR 87
           E EL +L+DY +R  NR+R
Sbjct: 117 EPELNKLIDYVQRTINRRR 135

[Close]

BLAST of CmaCh12G005420 vs. TrEMBL
Match: A0A0A0LRC3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359900 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 2.6e-17
Identity = 43/87 (49.43%), Postives = 65/87 (74.71%), Query Frame = 1


Query: 1  MSSVSNRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADA 60
          M++  ++++L+R+E++EI  EHD+DGDG+L+ QEVM+A   +G M+ + KAHYG+A AD 
Sbjct: 1  MATSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGVMMSFQKAHYGVAFADK 60

Query: 61 DGDGLIDED--ELERLVDYAERFQNRK 86
          DGDG +D +  E++ LVDY  RFQ  K
Sbjct: 61 DGDGAVDLEGGEIDSLVDYVMRFQASK 87

[Close]

BLAST of CmaCh12G005420 vs. TAIR10
Match: AT1G76650.1 (AT1G76650.1 calmodulin-like 38)

HSP 1 Score: 47.4 bits (111), Expect = 5.4e-06
Identity = 22/62 (35.48%), Postives = 39/62 (62.90%), Query Frame = 1


Query: 14  EIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADADGDGLIDEDELER 73
           E++ +F   D + DG++S +E+ K+F  LG  L   +A   + L+D DGDG++D +E  +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 74  LV 76
           L+
Sbjct: 103 LI 104

[Close]

BLAST of CmaCh12G005420 vs. TAIR10
Match: AT1G76640.1 (AT1G76640.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 46.6 bits (109), Expect = 9.2e-06
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1


Query: 2  SSVSNRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADAD 61
          SS    +     +++ +F   D + DG++S +E+ K+F  LG  +   +A   + L+D D
Sbjct: 10 SSFMKFLEEKNRDLEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDID 69

Query: 62 GDGLIDEDELERLVDYAERFQNRKR 87
          GDG++D +E   L+   + F   ++
Sbjct: 70 GDGMLDINEFALLIKGNDEFTEEEK 94

[Close]

BLAST of CmaCh12G005420 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 128.3 bits (321), Expect = 6.8e-27
Identity = 56/87 (64.37%), Postives = 77/87 (88.51%), Query Frame = 1


Query: 1  MSSVSNRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADA 60
          MS  + R++LSRE+IKEIFREHDIDGDG+LSM E++KAFG  G +LP++KAHYGMA AD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGLIDEDELERLVDYAERFQNRKRC 88
          +GDG I+E+EL++LVDYA++FQ++++C
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of CmaCh12G005420 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 126.3 bits (316), Expect = 2.6e-26
Identity = 55/83 (66.27%), Postives = 75/83 (90.36%), Query Frame = 1


Query: 5   SNRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADADGDG 64
           S R++LSRE+IKEIFREHDIDGDG+LSM E++KAFG  G +LP++KAHYGMA AD +GDG
Sbjct: 86  STRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 145

Query: 65  LIDEDELERLVDYAERFQNRKRC 88
            I+E+EL++LVDYA++FQ++++C
Sbjct: 146 FIEEEELDKLVDYAQKFQHKRKC 168

[Close]

BLAST of CmaCh12G005420 vs. NCBI nr
Match: gi|700207406|gb|KGN62525.1| (hypothetical protein Csa_2G359940 [Cucumis sativus])

HSP 1 Score: 120.6 bits (301), Expect = 1.4e-24
Identity = 54/81 (66.67%), Postives = 68/81 (83.95%), Query Frame = 1


Query: 6  NRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADADGDGL 65
          ++   SREE+KEIFREHDIDGDG LS+ E++KA GFLG  +P++KAHYGMA  D +GDGL
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 IDEDELERLVDYAERFQNRKR 87
          I EDEL++L+DYAERFQ R+R
Sbjct: 67 ISEDELDKLIDYAERFQKRRR 87

[Close]

BLAST of CmaCh12G005420 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 100.9 bits (250), Expect = 1.2e-18
Identity = 47/81 (58.02%), Postives = 65/81 (80.25%), Query Frame = 1


Query: 5   SNRMRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADADGDG 64
           ++ M+ SRE+IKEIF+ HD D DG L+++EV KAF FLG +LP +KA++GM  ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  LIDEDELERLVDYAERFQNRK 86
           LI +DELE+LVDYA +F N++
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of CmaCh12G005420 vs. NCBI nr
Match: gi|659089535|ref|XP_008445562.1| (PREDICTED: probable calcium-binding protein CML36 [Cucumis melo])

HSP 1 Score: 99.4 bits (246), Expect = 3.4e-18
Identity = 49/79 (62.03%), Postives = 57/79 (72.15%), Query Frame = 1


Query: 8   MRLSREEIKEIFREHDIDGDGQLSMQEVMKAFGFLGCMLPYHKAHYGMALADADGDGLID 67
           M+L+REEIKEIF   DIDGDG LS  EVM+AFG +G   P  K HY MA AD DGDGLI 
Sbjct: 57  MKLTREEIKEIFVGFDIDGDGFLSKNEVMQAFGMMGSYSPLLKTHYAMACADEDGDGLIS 116

Query: 68  EDELERLVDYAERFQNRKR 87
           E EL +L+DY +R  NR+R
Sbjct: 117 ECELNKLIDYVQRTNNRRR 135

The following BLAST results are available for this feature:

BLAST of CmaCh12G005420 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML23_ORYSJ	3.9e-06	40.85	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica GN=CML23 ...	[more]

BLAST of CmaCh12G005420 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC1_CUCSA	4.7e-27	64.37	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	9.9e-25	66.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	8.1e-19	58.02	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0LP46_CUCSA	1.2e-17	60.76	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358880 PE=4 SV=1	[more]
A0A0A0LRC3_CUCSA	2.6e-17	49.43	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359900 PE=4 SV=1	[more]

BLAST of CmaCh12G005420 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76650.1	5.4e-06	35.48	calmodulin-like 38	[more]
AT1G76640.1	9.2e-06	27.06	Calcium-binding EF-hand family protein	[more]

BLAST of CmaCh12G005420 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207397\|gb\|KGN62516.1\|	6.8e-27	64.37	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	2.6e-26	66.27	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700207406\|gb\|KGN62525.1\|	1.4e-24	66.67	hypothetical protein Csa_2G359940 [Cucumis sativus]	[more]
gi\|700194513\|gb\|KGN49690.1\|	1.2e-18	58.02	hypothetical protein Csa_5G068170 [Cucumis sativus]	[more]
gi\|659089535\|ref\|XP_008445562.1\|	3.4e-18	62.03	PREDICTED: probable calcium-binding protein CML36 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G005420.1	CmaCh12G005420.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 14..39 score: 8.
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 57..75 score: 1.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 50..78 score: 39.0coord: 14..42 score: 0.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 10..45 score: 14.486coord: 46..81 score: 8
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 10..75 score: 1.9
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 8..74 score: 3.49
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35 score: -coord: 59..71 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 3..78 score: 2.3
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 3..78 score: 2.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh12G005420	CsGy2G019080	Cucumber (Gy14) v2	cgybcmaB179

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh12G005420	Silver-seed gourd	carcmaB0100
CmaCh12G005420	Silver-seed gourd	carcmaB0249
CmaCh12G005420	Silver-seed gourd	carcmaB1067
CmaCh12G005420	Cucumber (Chinese Long) v3	cmacucB0203
CmaCh12G005420	Cucumber (Chinese Long) v3	cmacucB0215
CmaCh12G005420	Watermelon (97103) v2	cmawmbB194
CmaCh12G005420	Wax gourd	cmawgoB0194
CmaCh12G005420	Cucurbita maxima (Rimu)	cmacmaB199
CmaCh12G005420	Cucurbita maxima (Rimu)	cmacmaB182
CmaCh12G005420	Cucumber (Gy14) v1	cgycmaB0638
CmaCh12G005420	Cucurbita moschata (Rifu)	cmacmoB176
CmaCh12G005420	Cucurbita moschata (Rifu)	cmacmoB199
CmaCh12G005420	Wild cucumber (PI 183967)	cmacpiB175
CmaCh12G005420	Cucumber (Chinese Long) v2	cmacuB174
CmaCh12G005420	Cucumber (Chinese Long) v2	cmacuB186
CmaCh12G005420	Melon (DHL92) v3.5.1	cmameB156
CmaCh12G005420	Melon (DHL92) v3.5.1	cmameB182
CmaCh12G005420	Watermelon (Charleston Gray)	cmawcgB160
CmaCh12G005420	Watermelon (Charleston Gray)	cmawcgB169
CmaCh12G005420	Watermelon (97103) v1	cmawmB148
CmaCh12G005420	Watermelon (97103) v1	cmawmB159
CmaCh12G005420	Cucurbita pepo (Zucchini)	cmacpeB179
CmaCh12G005420	Cucurbita pepo (Zucchini)	cmacpeB203
CmaCh12G005420	Bottle gourd (USVL1VR-Ls)	cmalsiB184
CmaCh12G005420	Cucumber (Gy14) v2	cgybcmaB479
CmaCh12G005420	Melon (DHL92) v3.6.1	cmamedB170
CmaCh12G005420	Melon (DHL92) v3.6.1	cmamedB198