CmaCh12G005330 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh12G005330
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCalcium-binding EF-hand family protein
LocationCma_Chr12 : 2762847 .. 2764103 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGTCTCCATACTCCAAACTTGGGCCGAGAATGAAGCAAATGAATTGATGAAGAAGTATGACAAAAACGGTGATGGTATTCTCACTAAAGAAGAGCTACAAGTCTTTCTCCGTGATGTTCGGGGATCAACTCAACTCAACTATCAAGGGTTTTTATCTAAGATTCCAAAAACAAACGACAAGGGTGCACACAAGCTTAGCTCAAATACTCATACAAACCCAAAAATACAAGTACAAGACAAAGTTACAAAATCCTCGACGAAAGTTCTTAGTGAAAAGGTATCTAATGTCTCACTTAGTAGAGAGCAGATAAAGAAGATTTTCAAATATTATGACAGCGACAAAGACGGTTTTCTCAACATTAGAGAGGTAACAAAAGCATTTAGTTTTCTAGGCTCAATTAATCCATTCTACAAGGCTCAGTACGCTATGACTTTTGCTGATGCTAATAACGATGGTCTTATTAGTGAAACTGAGCTTGAAAAACTCATAGATTATGCCTGTAAAATCATCAAGAAGAAATGAGCTTGTAAGCTACTTTCTCATGAATTCCACGTTGTGATATCTCATGGTTGGAAATAAAGAATTTATTAATTTATTTATTTATTTTATCATTATTTTTATTTTTATTTTATTTTTTTATTTTTTGTCAATTTGTCTTTGTGATGATAATTTGTCTTTTTGTTAAACCATCTATTTAATAGCCAATAGATGATAATATCTCTGAACAGGTCAATGGTTTAATCTCCAATGCTGGTTTGTATATAACAATCAGTCAAAAAGATGGCCTTGAGTAAGCCATGAGTAATAGCACGTAACAAAATAGATATGTTAATAGTGTTAATGGTATACTTAAACCAATTAAAATAAGGCCTTCGTATTCTAATATAAGTATGAATGGACGTTTATGCCTCCGTGGTCTTGTAAGTAGGACCTTCGTATTCTAAGATAAATATTATTATTTTTTATATAAAACTTGAATTTGTTTGGATCATGATTATTCGGTGTCTGTTCTCTTTTTTTTCATGAGTAAACTCTGTTTTTTACATACCCATCGTAGTAGTTTATAATAAATCTAAAAAGTTAAGTTGTTACAATCTAATTCAAAGAATGCGAACTAATTACTCTACAAGTAGTTAGTAGAGGAAGAATTTTGACATCGTAATCTTATTCCTTTTGTAGGTGAATACAACATTACTTTTAGTTATTATTTTTTATTATAGTTTTTTAAAGGGTTTTGAGAATCTCGTTTAA

mRNA sequence

ATGTCTGTCTCCATACTCCAAACTTGGGCCGAGAATGAAGCAAATGAATTGATGAAGAAGTATGACAAAAACGGTGATGGTATTCTCACTAAAGAAGAGCTACAAGTCTTTCTCCGTGATGTTCGGGGATCAACTCAACTCAACTATCAAGGGTTTTTATCTAAGATTCCAAAAACAAACGACAAGGGTGCACACAAGCTTAGCTCAAATACTCATACAAACCCAAAAATACAAGTACAAGACAAAGTTACAAAATCCTCGACGAAAGTTCTTAGTGAAAAGGTATCTAATGTCTCACTTAGTAGAGAGCAGATAAAGAAGATTTTCAAATATTATGACAGCGACAAAGACGGTTTTCTCAACATTAGAGAGGGTTTTGAGAATCTCGTTTAA

Coding sequence (CDS)

ATGTCTGTCTCCATACTCCAAACTTGGGCCGAGAATGAAGCAAATGAATTGATGAAGAAGTATGACAAAAACGGTGATGGTATTCTCACTAAAGAAGAGCTACAAGTCTTTCTCCGTGATGTTCGGGGATCAACTCAACTCAACTATCAAGGGTTTTTATCTAAGATTCCAAAAACAAACGACAAGGGTGCACACAAGCTTAGCTCAAATACTCATACAAACCCAAAAATACAAGTACAAGACAAAGTTACAAAATCCTCGACGAAAGTTCTTAGTGAAAAGGTATCTAATGTCTCACTTAGTAGAGAGCAGATAAAGAAGATTTTCAAATATTATGACAGCGACAAAGACGGTTTTCTCAACATTAGAGAGGGTTTTGAGAATCTCGTTTAA

Protein sequence

MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSKIPKTNDKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDGFLNIREGFENLV
Homology
[Close]
BLAST of CmaCh12G005330 vs. TrEMBL
Match: A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 3.3e-32
Identity = 83/126 (65.87%), Postives = 96/126 (76.19%), Query Frame = 1 

Query: 1   MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSK--IPK 60
           MSVS LQ WA+NEANEL+KKYD+NGDG+LTKEELQ FLRD RGSTQLN    +S   +PK
Sbjct: 1   MSVSHLQAWAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPK 60

Query: 61  TNDKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDG 120
           TN+  A K+SSNT   PK Q+ DK   SS      K SNV LSREQIK+IFKY+DS+KDG
Sbjct: 61  TNENLAQKVSSNTCITPKKQLLDKAQGSS------KQSNVPLSREQIKEIFKYHDSNKDG 120

Query: 121 FLNIRE 125
           FL+IRE
Sbjct: 121 FLSIRE 120

[Close]
BLAST of CmaCh12G005330 vs. TrEMBL
Match: A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.6e-18
Identity = 57/116 (49.14%), Postives = 76/116 (65.52%), Query Frame = 1 

Query: 10  AENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNY-QGFLSKIPKTNDKGAHKLS 69
           A+ EA++LMKKY++NG+G++    L  F  + RGSTQ+N  +   +K  K ND  AHKLS
Sbjct: 38  AKYEASKLMKKYEQNGEGVI----LSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLS 97

Query: 70  SNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDGFLNIRE 125
           SN       Q++ K      +V   K SN+  SREQIK+IF+YYDSD+DGFLNIRE
Sbjct: 98  SNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIRE 149

[Close]
BLAST of CmaCh12G005330 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.3e-16
Identity = 58/125 (46.40%), Postives = 78/125 (62.40%), Query Frame = 1 

Query: 1   MSVSILQ-TWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSKIPKT 60
           M +S LQ + A  EA+ L+K+Y+KNGD +  K        DVR STQ+  +  + KI K 
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKA-----FDDVRSSTQIGNRESVLKILKP 60

Query: 61  NDKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDGF 120
           +D  AHKL S  H  P  +   K  KS T+VL  K SN+SLSRE+IK+IF+Y+D++ DGF
Sbjct: 61  SDGVAHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGF 120

Query: 121 LNIRE 125
           LN  E
Sbjct: 121 LNRME 120

[Close]
BLAST of CmaCh12G005330 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 7.0e-11
Identity = 52/129 (40.31%), Postives = 79/129 (61.24%), Query Frame = 1 

Query: 1   MSVSILQ-TWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSK---I 60
           M +S LQ + A+ EA++LMKK++ N +G+     L+ F      ST++N +  ++    +
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGV----PLKAFNGIPPASTKINNKQAMATEIIL 60

Query: 61  PKTNDKGAHKLSSNTHTNPKIQV-QDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSD 120
             +N   AH L+SN +  P  Q+   K   ++T VL  K SN+  SREQIK+IF+Y+DSD
Sbjct: 61  KPSNHPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSD 120

Query: 121 KDGFLNIRE 125
            DGFLN+RE
Sbjct: 121 NDGFLNLRE 125

[Close]
BLAST of CmaCh12G005330 vs. NCBI nr
Match: gi|449470190|ref|XP_004152801.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus])

HSP 1 Score: 146.0 bits (367), Expect = 4.7e-32
Identity = 83/126 (65.87%), Postives = 96/126 (76.19%), Query Frame = 1 

Query: 1   MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSK--IPK 60
           MSVS LQ WA+NEANEL+KKYD+NGDG+LTKEELQ FLRD RGSTQLN    +S   +PK
Sbjct: 1   MSVSHLQAWAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPK 60

Query: 61  TNDKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDG 120
           TN+  A K+SSNT   PK Q+ DK   SS      K SNV LSREQIK+IFKY+DS+KDG
Sbjct: 61  TNENLAQKVSSNTCITPKKQLLDKAQGSS------KQSNVPLSREQIKEIFKYHDSNKDG 120

Query: 121 FLNIRE 125
           FL+IRE
Sbjct: 121 FLSIRE 120

[Close]
BLAST of CmaCh12G005330 vs. NCBI nr
Match: gi|659089539|ref|XP_008445564.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo])

HSP 1 Score: 122.5 bits (306), Expect = 5.5e-25
Identity = 74/126 (58.73%), Postives = 88/126 (69.84%), Query Frame = 1 

Query: 1   MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSK--IPK 60
           MSVS LQ WA+ EA+EL+KKYD+NGDG+LTKEELQ FL D R    L    F+S   IPK
Sbjct: 1   MSVSHLQAWAKIEASELIKKYDRNGDGVLTKEELQSFLHDAR----LKNIRFVSSSTIPK 60

Query: 61  TNDKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDG 120
           TN+    K+SSNT   PK Q+ DK   S       + SNV LSREQIK+IFKY+DS+KDG
Sbjct: 61  TNENLDQKVSSNTRIKPKKQLLDKAQDSL------RQSNVPLSREQIKEIFKYHDSNKDG 116

Query: 121 FLNIRE 125
           FL+IRE
Sbjct: 121 FLSIRE 116

[Close]
BLAST of CmaCh12G005330 vs. NCBI nr
Match: gi|700194512|gb|KGN49689.1| (hypothetical protein Csa_5G067670 [Cucumis sativus])

HSP 1 Score: 99.0 bits (245), Expect = 6.5e-18
Identity = 57/116 (49.14%), Postives = 76/116 (65.52%), Query Frame = 1 

Query: 10  AENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNY-QGFLSKIPKTNDKGAHKLS 69
           A+ EA++LMKKY++NG+G++    L  F  + RGSTQ+N  +   +K  K ND  AHKLS
Sbjct: 38  AKYEASKLMKKYEQNGEGVI----LSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLS 97

Query: 70  SNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDGFLNIRE 125
           SN       Q++ K      +V   K SN+  SREQIK+IF+YYDSD+DGFLNIRE
Sbjct: 98  SNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIRE 149

[Close]
BLAST of CmaCh12G005330 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 91.7 bits (226), Expect = 1.0e-15
Identity = 58/125 (46.40%), Postives = 78/125 (62.40%), Query Frame = 1 

Query: 1   MSVSILQ-TWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSKIPKT 60
           M +S LQ + A  EA+ L+K+Y+KNGD +  K        DVR STQ+  +  + KI K 
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKA-----FDDVRSSTQIGNRESVLKILKP 60

Query: 61  NDKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDGF 120
           +D  AHKL S  H  P  +   K  KS T+VL  K SN+SLSRE+IK+IF+Y+D++ DGF
Sbjct: 61  SDGVAHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGF 120

Query: 121 LNIRE 125
           LN  E
Sbjct: 121 LNRME 120

[Close]
BLAST of CmaCh12G005330 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-10
Identity = 52/129 (40.31%), Postives = 79/129 (61.24%), Query Frame = 1 

Query: 1   MSVSILQ-TWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSK---I 60
           M +S LQ + A+ EA++LMKK++ N +G+     L+ F      ST++N +  ++    +
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGV----PLKAFNGIPPASTKINNKQAMATEIIL 60

Query: 61  PKTNDKGAHKLSSNTHTNPKIQV-QDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSD 120
             +N   AH L+SN +  P  Q+   K   ++T VL  K SN+  SREQIK+IF+Y+DSD
Sbjct: 61  KPSNHPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSD 120

Query: 121 KDGFLNIRE 125
            DGFLN+RE
Sbjct: 121 NDGFLNLRE 125

The following BLAST results are available for this feature:
BLAST of CmaCh12G005330 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of CmaCh12G005330 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LRC8_CUCSA3.3e-3265.87Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1[more]
A0A0A0KLF3_CUCSA4.6e-1849.14Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1[more]
A0A0A0KMQ1_CUCSA7.3e-1646.40Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1[more]
A0A0A0KIY4_CUCSA7.0e-1140.31Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1[more]
BLAST of CmaCh12G005330 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of CmaCh12G005330 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449470190|ref|XP_004152801.1|4.7e-3265.87PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus][more]
gi|659089539|ref|XP_008445564.1|5.5e-2558.73PREDICTED: probable calcium-binding protein CML11 [Cucumis melo][more]
gi|700194512|gb|KGN49689.1|6.5e-1849.14hypothetical protein Csa_5G067670 [Cucumis sativus][more]
gi|700194510|gb|KGN49687.1|1.0e-1546.40hypothetical protein Csa_5G067410 [Cucumis sativus][more]
gi|700194513|gb|KGN49690.1|1.0e-1040.31hypothetical protein Csa_5G068170 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G005330.1CmaCh12G005330.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 15..124
score: 2.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 13..41
score: 3.0E-5coord: 104..129
score:
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 9..44
score: 12.895coord: 100..130
score: 9
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 8..38
score: 2.7E-12coord: 96..124
score: 2.7
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 6..55
score: 3.55E-13coord: 97..124
score: 3.55
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 22..34
scor

Synteny