CmaCh12G004270 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh12G004270 vs. Swiss-Prot
Match: KN7F_ORYSJ (Kinesin-like protein KIN-7F OS=Oryza sativa subsp. japonica GN=KIN7F PE=1 SV=2)

HSP 1 Score: 737.6 bits (1903), Expect = 1.7e-211
Identity = 469/986 (47.57%), Postives = 628/986 (63.69%), Query Frame = 1

Query: 1   MEAKEPIQGVIQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDR 60
           M+  E + G      G  E I VS+RLRPL++KE+ + D S+W C+N+ +II RST PDR
Sbjct: 15  MDGGEVVNGGGGGGVGKLERILVSVRLRPLSDKEIARGDPSEWECINDTTIISRSTFPDR 74

Query: 61  AMYPQSYTFDRVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGIT 120
              P +Y+FDRVF  D  T +VY++GAKEV LSVV+GINS+IFAYGQTSSGKTYTM GIT
Sbjct: 75  PSAPTAYSFDRVFRSDCDTNEVYKQGAKEVALSVVSGINSSIFAYGQTSSGKTYTMTGIT 134

Query: 121 EYSVADIYNYMETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLT 180
           EY+VADIY+Y+  H+ER +VLKFSAIEIYNE VRDLLS EN PLRL DD EKGT VE LT
Sbjct: 135 EYTVADIYDYIGKHEERAFVLKFSAIEIYNEVVRDLLSAENTPLRLWDDAEKGTYVENLT 194

Query: 181 EETLKDRNHLQELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLT 240
           E  L+D NHL+EL+S CE QRK GET LNE SSRSHQIL+LTIESSAR+F   +  ++L 
Sbjct: 195 EVVLRDWNHLKELISVCEAQRKTGETYLNENSSRSHQILKLTIESSAREFLGKDKSTTLV 254

Query: 241 ATVNFVDLAGSERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLT 300
           A+VNFVDLAGSERASQ +SAG RLKEGCHINRSLL+LGTVIRKLSK R GH+PYRDSKLT
Sbjct: 255 ASVNFVDLAGSERASQALSAGARLKEGCHINRSLLTLGTVIRKLSKVRNGHIPYRDSKLT 314

Query: 301 RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ 360
           RILQ SLGGN RTAIICTMSPARSH+EQSRNTLLFA+CAKEV TNA VNVV+SDKALVKQ
Sbjct: 315 RILQPSLGGNARTAIICTMSPARSHMEQSRNTLLFASCAKEVVTNAQVNVVMSDKALVKQ 374

Query: 361 LQKELARLESEMKNIKPLPVKGDS-NSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENL 420
           LQKELARLESE++     P    S  SL+KEK+  I +M+KEIKEL  QRDLAQ R+++L
Sbjct: 375 LQKELARLESELR----CPASYSSLESLVKEKDNQIRKMEKEIKELKLQRDLAQSRLQDL 434

Query: 421 LHSVGEDRIFKRSESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSS 480
           L  VG++ +    +S+V       +  + +T  +  S T  + V D V+           
Sbjct: 435 LQVVGDNHVHVSKQSSVS--GRNFTFDVPQTCEDEQSTTESSEVVDSVQ----------- 494

Query: 481 LKTFDTFNGQG----QEENSPHKV--NPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEVA 540
                 F  QG    Q E+ P +   N  F+T          S+P  +G  P N  + ++
Sbjct: 495 -----NFRFQGRRVAQREHKPQQAENNVQFTTPSR----YSVSSPPFSGMLPTNRSDHLS 554

Query: 541 QRVRANSEDSYKDVQCIELEESNQTPNENVELALA---RLED-NEGQMISTSR-TNQVTS 600
           Q    +S+D  K+V+CIE  E+    NE +E +      L+D N G  +  +  +N   +
Sbjct: 555 QISNEDSDDICKEVRCIETNETGG--NECLESSAVGSNSLQDPNAGSSMHINNDSNSSMN 614

Query: 601 PQRKNKEIVTINKDYVSGGFMPNTAEPKRTLNCIVNFYPAEQSFSSIEAAKDRFQNLKLT 660
            + +++  VT+ +       + N  +P    N + +   + ++ SS +          L 
Sbjct: 615 SRLRDESPVTLEQH------LENVRKP--FANIVKDLGSSTRNSSSSKV---------LG 674

Query: 661 RSKSCLTVLMELPPSTWVEKPETDKKAQHNGSEINFSGLAEGSRRKRGLSCGNLETEESQ 720
           RS+SC +    L  S+  E  E D     N S I+F+G  +  +R+      + E+E   
Sbjct: 675 RSRSCRS----LTGSSLFEDLEKDDCTPPNRSFIDFAGRPQNCQRRGSALNYDAESETLS 734

Query: 721 SVCNRPSED-------KALEIIEEDDDDNTSVLNFATGKKGKSKNRLRKRSTHGSRLGRT 780
              +  SE        KA   +   D + T +  F    K  ++ + +K+      LG +
Sbjct: 735 RAGSMLSEITTTRDGLKANSSVA-GDTEFTGIGEFVAELKEMAQVQYQKQ------LGHS 794

Query: 781 PKREEAKETTQDVGLEEEQD-FQSHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILF 840
              + A+ T + VGL+   D  QS S W LEF+ +Q++II+ W ACNV LVHR+YFF+LF
Sbjct: 795 GNGDLAEGTIRSVGLDPITDALQSPSRWPLEFEKKQQEIIDFWHACNVSLVHRTYFFLLF 854

Query: 841 KGDPSDAVYMEVELRRLFFIREAMSRSTNGFGRNDAITQASSLKTLNREREMLVKRIKKK 900
           KGDP+D++YMEVELRRL F+++  S        N ++   SS K L REREML ++++++
Sbjct: 855 KGDPADSIYMEVELRRLSFLKDTYSNGAIASIPNTSL--VSSAKKLQREREMLCRQMQRR 914

Query: 901 FSVKERDTLYKKWGIDLKTKQRSIQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVS 960
            S++ER+++Y KWG+ L +K+R +Q+A+ LWT TKD +H+ ESA+LVA+L+G +EP +  
Sbjct: 915 LSIEERESMYTKWGVSLASKRRRLQVARCLWTETKDLEHVRESASLVARLIGLLEPGKAL 942

Query: 961 REMFGLSFSLQSLDQRSF-PWKRNMS 966
           REMFGLSF+ Q   +RS+  W+   S
Sbjct: 975 REMFGLSFAPQQFTRRSYNSWRYGRS 942

BLAST of CmaCh12G004270 vs. Swiss-Prot
Match: KN7H_ORYSJ (Kinesin-like protein KIN-7H OS=Oryza sativa subsp. japonica GN=KIN7H PE=2 SV=2)

HSP 1 Score: 698.7 bits (1802), Expect = 8.7e-200
Identity = 448/1000 (44.80%), Postives = 615/1000 (61.50%), Query Frame = 1

Query: 3   AKEPIQGVIQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDRAM 62
           A+E        +   EE I VS+RLRPLN +E    DS DW C++  +++FRST+P+RAM
Sbjct: 4   AEEEAAAAWDKAEAKEERIMVSVRLRPLNGREA--GDSCDWECISPTTVMFRSTVPERAM 63

Query: 63  YPQSYTFDRVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEY 122
           +P +YT+DRVFG DS+T+QVYEEGAKEV LSVV+GINS+IFAYGQTSSGKTYTM GITEY
Sbjct: 64  FPTAYTYDRVFGPDSSTRQVYEEGAKEVALSVVSGINSSIFAYGQTSSGKTYTMTGITEY 123

Query: 123 SVADIYNYMETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEE 182
           SV DIY+Y+E H ERE++L+FSAIEIYNEAVRDLLS +  PLRLLDDPEKGT VEKLTEE
Sbjct: 124 SVLDIYDYIEKHPEREFILRFSAIEIYNEAVRDLLSHDTTPLRLLDDPEKGTTVEKLTEE 183

Query: 183 TLKDRNHLQELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTAT 242
           TL+D++HL+ LL+ CE QR+IGET LNE SSRSHQILRLTIESS R++    + S+L A 
Sbjct: 184 TLRDKDHLRNLLAVCEAQRQIGETALNETSSRSHQILRLTIESSTRQYLGRGNSSTLVAC 243

Query: 243 VNFVDLAGSERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRI 302
           VNFVDLAGSERASQT SAG RLKEG HINRSLL+LG V+R+LSKGR GH+PYRDSKLTRI
Sbjct: 244 VNFVDLAGSERASQTASAGVRLKEGSHINRSLLTLGKVVRQLSKGRNGHIPYRDSKLTRI 303

Query: 303 LQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQ 362
           LQ+SLGGN RTAIICTMSPARSH+EQSRNTLLFATCAKEV TNA VNVV+SDKALVK LQ
Sbjct: 304 LQSSLGGNARTAIICTMSPARSHIEQSRNTLLFATCAKEVVTNAQVNVVMSDKALVKHLQ 363

Query: 363 KELARLESEMKNIKPLPVKGDSNS-LLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLH 422
           +EL RL+SE+K   P P    +++  L+EK+  I++++K++KEL  +RD  + +++ LL 
Sbjct: 364 RELERLQSEIK--FPAPASCTTHAEALREKDAQIKKLEKQLKELMEERDTVKSQLDCLLK 423

Query: 423 SVGED----RIFKRSESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLD-LRSD 482
           S  +D    R+ KR +   +           E    + +  VP    D    +   + SD
Sbjct: 424 SDCDDHSDGRVAKRWDEHSRSSESFARNASEEAFSVSDTSGVPYQDQDNAVFNGSYVFSD 483

Query: 483 DSSLKTFDTFNGQGQEENSPHK-VNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEVAQR 542
           D     F        EE    K ++P    SH       SS+  +     Y+  E  ++ 
Sbjct: 484 DRDDIVFPVQTVDLPEETKHEKFMSPWHPPSH------HSSSDCIES---YHMTEAASRT 543

Query: 543 VRANSEDSYKDVQCIELEESNQTPNENVELALARLEDNEGQMISTSRTNQVTSPQRKNKE 602
               SE+  ++VQCI++ E  ++ +   +L L   +D E Q      + +      +++E
Sbjct: 544 ASEVSEEHCREVQCIDIHEHRRSTSHKFDLLLP--QDTEFQTPELEISKEAVPQPDEDQE 603

Query: 603 IVTINK-----------DYVSGGFMPNTAEPKRTLNCIVNFYPAEQSFS-SIE------- 662
           + +I             +      + +T E   T +  V  Y  + + S  I+       
Sbjct: 604 LESITNRMEDPTRMCPVEEEQQDEIVDTCESNGTTDNDVKLYTCDSNISFDIQKPYPNGC 663

Query: 663 --------AAKDRFQNLKLTRSKSCLTVLMELPPSTWVEKPETDKKAQHNGSEINFSGLA 722
                   ++KDR     L+RSKSC    M + P++W +  E   +   N    +     
Sbjct: 664 LTVKRCILSSKDR----ALSRSKSCRASFM-IIPNSWFDDSEYTSQTPPNEILKHTPRRF 723

Query: 723 EGSRRKRGLSCGNLETEESQSVCNRPSEDKALEIIEEDDDDNTSVLNFATGKKGKSKN-- 782
           +  RR       N  + +        S D+ ++ +   D+    +      ++  + +  
Sbjct: 724 DKVRRSLYPENDNPSSVDRSEFSGEVSSDEVVKDMSTIDEVAKDMCPSDAEQETLTSDIS 783

Query: 783 ---RLRKR-STHGSRLGRTPKREEAKE------TTQDVGLEEEQDFQSHSDWLLEFQGQQ 842
              +L+K  S H   L     ++  ++      T +DVG++      S S W ++F+  +
Sbjct: 784 CLTKLKKTDSDHEDELDEYQDQQSIRDGSTTLRTVKDVGIDSSLS-ASPSRWPIDFEKMR 843

Query: 843 RDIIELWDACNVPLVHRSYFFILFKGDPSDAVYMEVELRRLFFIREAMSRSTNGFGRNDA 902
           ++II+LW  CN P+VHR+YFF+LFKGDP+D +YMEVE RRL FIR + S S  G   N A
Sbjct: 844 QEIIQLWHECNAPIVHRTYFFLLFKGDPADNIYMEVEHRRLSFIRRSFSASPAGGELNSA 903

Query: 903 ITQASSLKTLNREREMLVKRIKKKFSVKERDTLYKKWGIDLKTKQRSIQLAKKLWTRTKD 957
           +   SSLK L RER+ML K++ KK +  E++ +Y +WGIDL +KQR +QL++ +WT+T D
Sbjct: 904 V--VSSLKNLRRERDMLYKQMLKKLTNGEKERVYARWGIDLSSKQRRLQLSRLVWTQT-D 963

BLAST of CmaCh12G004270 vs. Swiss-Prot
Match: KN7G_ARATH (Kinesin-like protein KIN-7G OS=Arabidopsis thaliana GN=KIN7G PE=2 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 2.5e-154
Identity = 277/417 (66.43%), Postives = 357/417 (85.61%), Query Frame = 1

Query: 11  IQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRS--TLPDRAMYPQSYT 70
           +Q S+G EE I VS+RLRPLN +E  +ND +DW C+N+ ++I+RS  ++ +R+MYP +YT
Sbjct: 8   MQGSSGREEKIFVSVRLRPLNVRERARNDVADWECINDETVIYRSHLSISERSMYPTAYT 67

Query: 71  FDRVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIY 130
           FDRVFG + +T++VY++GAKEV LSVV+G+++++FAYGQTSSGKTYTM GIT+Y++ADIY
Sbjct: 68  FDRVFGPECSTREVYDQGAKEVALSVVSGVHASVFAYGQTSSGKTYTMIGITDYALADIY 127

Query: 131 NYMETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRN 190
           +Y+E H ERE++LKFSA+EIYNE+VRDLLS +  PLR+LDDPEKGTVVEKLTEETL+D N
Sbjct: 128 DYIEKHNEREFILKFSAMEIYNESVRDLLSTDISPLRVLDDPEKGTVVEKLTEETLRDWN 187

Query: 191 HLQELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDL 250
           H +ELLS C  QR+IGET LNE SSRSHQILRLT+ES+AR++   +  S+LTATVNF+DL
Sbjct: 188 HFKELLSICIAQRQIGETALNEVSSRSHQILRLTVESTAREYLAKDKFSTLTATVNFIDL 247

Query: 251 AGSERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLG 310
           AGSERASQ++SAGTRLKEG HINRSLL+LGTVIRKLSKG+ GH+P+RDSKLTRILQ SLG
Sbjct: 248 AGSERASQSLSAGTRLKEGGHINRSLLTLGTVIRKLSKGKNGHIPFRDSKLTRILQTSLG 307

Query: 311 GNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARL 370
           GN RT+IICT+SPAR HVEQSRNTLLFA+CAKEV+TNA VNVV+SDKALV+ LQ+ELA+L
Sbjct: 308 GNARTSIICTLSPARVHVEQSRNTLLFASCAKEVTTNAQVNVVMSDKALVRHLQRELAKL 367

Query: 371 ESEMKNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGE 426
           ESE+ + +   V  D+ +LLKEK++ IE+++KE+ +L ++ + A  RIE+L   +GE
Sbjct: 368 ESELSSPRQALVVSDTTALLKEKDLQIEKLNKEVFQLAQELERAYSRIEDLQQIIGE 424

BLAST of CmaCh12G004270 vs. Swiss-Prot
Match: KN7H_ARATH (Kinesin-like protein KIN-7H OS=Arabidopsis thaliana GN=KIN7H PE=2 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 9.4e-154
Identity = 280/441 (63.49%), Postives = 362/441 (82.09%), Query Frame = 1

Query: 15  NGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRS--TLPDRAMYPQSYTFDRV 74
           +G +E I VS+R+RPLN+KE  +ND  DW C+NN +II+RS  ++ +R+MYP +YTFDRV
Sbjct: 13  SGTQEKIYVSVRMRPLNDKEKFRNDVPDWECINNTTIIYRSHLSISERSMYPSAYTFDRV 72

Query: 75  FGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYME 134
           F  +  T+QVYE+GAKEV  SVV+G+N+++FAYGQTSSGKTYTM+GIT+ ++ DIY Y++
Sbjct: 73  FSPECCTRQVYEQGAKEVAFSVVSGVNASVFAYGQTSSGKTYTMSGITDCALVDIYGYID 132

Query: 135 THQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHLQE 194
            H+ERE++LKFSA+EIYNE+VRDLLS +  PLRLLDDPEKGTVVEKLTEETL+D NH +E
Sbjct: 133 KHKEREFILKFSAMEIYNESVRDLLSTDTSPLRLLDDPEKGTVVEKLTEETLRDWNHFKE 192

Query: 195 LLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGSE 254
           LLS C+ QR+IGET LNE SSRSHQILRLT+ES AR+F  ++  S+LTATVNF+DLAGSE
Sbjct: 193 LLSVCKAQRQIGETALNEVSSRSHQILRLTVESIAREFSTNDKFSTLTATVNFIDLAGSE 252

Query: 255 RASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNGR 314
           RASQ++SAGTRLKEGCHINRSLL+LGTVIRKLSK +TGH+P+RDSKLTRILQ+SLGGN R
Sbjct: 253 RASQSLSAGTRLKEGCHINRSLLTLGTVIRKLSKEKTGHIPFRDSKLTRILQSSLGGNAR 312

Query: 315 TAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESEM 374
           TAIICTMSPAR HVEQSRNTLLFA+CAKEV+TNA VNVV+SDKALVK LQ+ELA+LESE+
Sbjct: 313 TAIICTMSPARIHVEQSRNTLLFASCAKEVTTNAQVNVVMSDKALVKHLQRELAKLESEL 372

Query: 375 KNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIFKRS 434
           ++     +  D+ +LL EK++ +E++ KE+ +L +Q + A+  I++L   V E++   + 
Sbjct: 373 RSPSQASIVSDTTALLTEKDLEVEKLKKEVFQLAQQLEQARSEIKDLRRMVEEEK--NQE 432

Query: 435 ESTVQIVPETMSETMSETVPE 454
           + T+    E ++  M    P+
Sbjct: 433 KETLSTETEGLNVLMEHQYPK 451

BLAST of CmaCh12G004270 vs. Swiss-Prot
Match: KN7C_ORYSJ (Kinesin-like protein KIN-7C OS=Oryza sativa subsp. japonica GN=KIN7C PE=2 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.1e-153
Identity = 287/426 (67.37%), Postives = 345/426 (80.99%), Query Frame = 1

Query: 1   MEAKEPIQGVIQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDR 60
           M A E + G    + G  + I+V +RLRPL+EKE+ + + ++W C+N+++++FRST PDR
Sbjct: 15  MGAAEAVNGGCGGA-GKMDRIQVLVRLRPLSEKEVARREPAEWECINDSTVMFRSTFPDR 74

Query: 61  AMYPQSYTFDRVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGIT 120
              P +YTFDRVF  D +TK+VYEEG KEV LSVV+GINS+IFAYGQTSSGKTYTM G+T
Sbjct: 75  PTAPTAYTFDRVFHSDCSTKEVYEEGVKEVALSVVSGINSSIFAYGQTSSGKTYTMTGVT 134

Query: 121 EYSVADIYNYMETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLT 180
           EY+VADIY+Y+  H+ER +VLKFSAIEIYNE +RDLLS EN PLRL DD EKGT VE LT
Sbjct: 135 EYTVADIYDYINKHEERAFVLKFSAIEIYNEVIRDLLSAENTPLRLWDDAEKGTYVENLT 194

Query: 181 EETLKDRNHLQELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLT 240
           E  L+D NHL+ L+S CE QR+ GET LNE SSRSHQILRLT+ESSAR+F   +  ++L 
Sbjct: 195 EVVLRDWNHLKGLISVCEAQRRTGETFLNEKSSRSHQILRLTVESSAREFLGKDKSTTLV 254

Query: 241 ATVNFVDLAGSERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLT 300
           A+ NFVDLAGSERASQ +SAGTRLKEGCHINRSLL+LGTVIRKLS G   H+PYRDSKLT
Sbjct: 255 ASANFVDLAGSERASQALSAGTRLKEGCHINRSLLALGTVIRKLSMGSNAHIPYRDSKLT 314

Query: 301 RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ 360
           RILQ SLGGN RTAIICT+SPA SH+EQSRNTLLF +CAKEV TNA VNVV+SDKALVK 
Sbjct: 315 RILQPSLGGNARTAIICTLSPATSHIEQSRNTLLFGSCAKEVVTNAQVNVVMSDKALVKH 374

Query: 361 LQKELARLESEMKNIKPLPVKGDS-NSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENL 420
           LQKELARLESE+++    PV+  S  +LLKEK+  I +M+KEIKEL  QRDLAQ R+++L
Sbjct: 375 LQKELARLESELRH----PVQSSSLETLLKEKDNQIRKMEKEIKELKSQRDLAQSRLQDL 434

Query: 421 LHSVGE 426
           L SVG+
Sbjct: 435 LQSVGD 435

BLAST of CmaCh12G004270 vs. TrEMBL
Match: A0A0A0LNQ8_CUCSA (Kinesin-like protein OS=Cucumis sativus GN=Csa_2G365120 PE=3 SV=1)

HSP 1 Score: 1455.7 bits (3767), Expect = 0.0e+00
Identity = 764/984 (77.64%), Postives = 853/984 (86.69%), Query Frame = 1

Query: 1   MEAKEPIQGVIQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDR 60
           M  +E I+GVI  SNGLEETIRVSIRLRPLNEKEL KNDSSDW C+N+ S++FRSTLP+R
Sbjct: 1   MGGEELIKGVINNSNGLEETIRVSIRLRPLNEKELMKNDSSDWECINSTSVMFRSTLPER 60

Query: 61  AMYPQSYTFDRVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGIT 120
           +++P SYTFDRVFG DSTTKQVYEEGA+EVVLSVVNGINSTIFAYGQTSSGKT+TMNG+T
Sbjct: 61  SLFPHSYTFDRVFGIDSTTKQVYEEGAREVVLSVVNGINSTIFAYGQTSSGKTFTMNGVT 120

Query: 121 EYSVADIYNYMETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLT 180
           ++SVADIY+Y+E+HQ+RE+VLKFSAIEIYNEAV+DLLS ENVPLRLLDDPEKGTVVEKLT
Sbjct: 121 QHSVADIYSYIESHQDREFVLKFSAIEIYNEAVKDLLSLENVPLRLLDDPEKGTVVEKLT 180

Query: 181 EETLKDRNHLQELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLT 240
           EE LKDRNHLQEL+SFCEVQRKIGET+LNE SSRSHQILRLTIESSARKFKKSES S+LT
Sbjct: 181 EEILKDRNHLQELISFCEVQRKIGETSLNETSSRSHQILRLTIESSARKFKKSESSSTLT 240

Query: 241 ATVNFVDLAGSERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLT 300
           ATVNFVDLAGSERASQT S GTRLKEGCHINRSLL+LGTVIRKLSKGR GH+PYRDSKLT
Sbjct: 241 ATVNFVDLAGSERASQTNSGGTRLKEGCHINRSLLTLGTVIRKLSKGRNGHIPYRDSKLT 300

Query: 301 RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ 360
           RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ
Sbjct: 301 RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ 360

Query: 361 LQKELARLESEMKNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLL 420
           LQKELARLESEMKN+KPLP+KGDSNSLLKEKE++IEQMDKEIKELTRQRDLAQYRIENLL
Sbjct: 361 LQKELARLESEMKNLKPLPLKGDSNSLLKEKELVIEQMDKEIKELTRQRDLAQYRIENLL 420

Query: 421 HSVGEDRIFKRSESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDD--- 480
           HSVGEDRIFK SESTV                         T+PDLV+LDLDLRSDD   
Sbjct: 421 HSVGEDRIFKLSESTVH------------------------TIPDLVDLDLDLRSDDSSF 480

Query: 481 -----SSLKTFDTFNGQGQEENSPHKVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEV 540
                SSLKTFDTF    QEENSPHK++PLF+ +H+DDFLLDSSTPELAGP PY +WEE+
Sbjct: 481 KAFDESSLKTFDTFT--AQEENSPHKIDPLFTMNHEDDFLLDSSTPELAGPDPYQDWEEI 540

Query: 541 AQRVRANSEDSYKDVQCIELEESNQTPNENVELALARLEDNEGQMISTSRTNQVTSPQRK 600
           A+RV ANSED  KDVQCIELEE  +  NEN +L LA  EDNEGQMIS+   N  T PQR+
Sbjct: 541 AERVHANSEDGCKDVQCIELEELKELVNENGDLTLATWEDNEGQMISSFDINPETYPQRR 600

Query: 601 NKEIVTINKDYVSGGFMPNTAEPKRTLNCIVNFYPAEQSFSSIEAAKDRFQNLKLTRSKS 660
           NKEI+ I+K +   G +P  +E  +TLNCIVN YP+EQSF+SIEAAK RFQNLKL RSKS
Sbjct: 601 NKEIIPISKGHTYDGLIPKASEIGKTLNCIVNLYPSEQSFNSIEAAKTRFQNLKLERSKS 660

Query: 661 CLTVLMELPPSTWVEKPETDKKAQHNGSEINFSGLAEGSRRKRGLSCG--------NLET 720
           CLTVLM +PPST +EK + DKK +  GS++NFSG AEGSRR+RGLSCG        NL+T
Sbjct: 661 CLTVLMTIPPSTLIEKVKEDKKIRTVGSDVNFSGKAEGSRRRRGLSCGNLGANLEANLDT 720

Query: 721 EESQSVCNRPSEDKALEIIEEDDDDNTSVLNFATGKKGKSKNRLRKRSTHGSRLGRTPKR 780
           ++SQSVC+R S+ K L+IIEEDDDDNTSVLNFATGK+GKSKNR++KRS  GSRLG   K+
Sbjct: 721 KDSQSVCSRCSDTKTLQIIEEDDDDNTSVLNFATGKRGKSKNRMKKRS--GSRLGSLSKK 780

Query: 781 EEAKETTQDVGLEEEQDFQSHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDP 840
           EE  E T +V  +EE++ Q+HS+W+LEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDP
Sbjct: 781 EEPIEATLEVQTQEEKELQAHSEWILEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDP 840

Query: 841 SDAVYMEVELRRLFFIREAMSRSTNGFGRNDAITQASSLKTLNREREMLVKRIKKKFSVK 900
           +DAVYMEVELRRLFFIREA+SRS NG GR DA+TQ SSLK LNRER+ML +R+KKKF+VK
Sbjct: 841 TDAVYMEVELRRLFFIREAISRSVNGSGRGDAVTQTSSLKALNRERDMLARRMKKKFTVK 900

Query: 901 ERDTLYKKWGIDLKTKQRSIQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVSREMF 960
           ERD LY KWGIDLKTKQRSIQ+A+ LW+RTKDFDHI+ESAALVAKL+GFVEP+QVSREMF
Sbjct: 901 ERDALYVKWGIDLKTKQRSIQVARMLWSRTKDFDHINESAALVAKLIGFVEPNQVSREMF 956

Query: 961 GLSFSLQSLDQRSFPWKRNMSLPF 969
           GLS SLQSLD RSF WKRNMSLPF
Sbjct: 961 GLSISLQSLDHRSFSWKRNMSLPF 956

BLAST of CmaCh12G004270 vs. TrEMBL
Match: B9R839_RICCO (Kinesin-like protein OS=Ricinus communis GN=RCOM_1596550 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 2.1e-237
Identity = 488/973 (50.15%), Postives = 650/973 (66.80%), Query Frame = 1

Query: 18  EETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDRAMYPQSYTFDRVFGCDS 77
           EE I VS+R+RPLNE+E+ +ND  DW C+NNN+IIF+S +PDR+M P +YTFDRVFG + 
Sbjct: 25  EEKIFVSVRMRPLNEREVARNDICDWECINNNTIIFKSNMPDRSMVPTAYTFDRVFGSEC 84

Query: 78  TTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYMETHQER 137
           +TKQVYEEGAKE+ L+ V+GINS+IFAYGQTSSGKTYTM+G+TEY+VADIY YM  H+ER
Sbjct: 85  STKQVYEEGAKEIALAAVSGINSSIFAYGQTSSGKTYTMDGVTEYAVADIYEYMNKHKER 144

Query: 138 EYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHLQELLSFC 197
           E+VLKFSA+EIYNEAVRDLLS ++ PLR+LDDPEKGTVVE+L EETL D NHLQELL+ C
Sbjct: 145 EFVLKFSAMEIYNEAVRDLLSSDSTPLRVLDDPEKGTVVERLIEETLIDWNHLQELLTIC 204

Query: 198 EVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGSERASQT 257
           E QR+IGET++NE SSRSHQ++RLTIESSAR++    + S L +TV+FVDLAGSERASQT
Sbjct: 205 EAQRQIGETSMNENSSRSHQVIRLTIESSAREYSGVGNSSILISTVHFVDLAGSERASQT 264

Query: 258 MSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNGRTAIIC 317
           ++ G RLKEG HINRSLL+LGTVIRKLSKG+ GH+PYRDSKLTRILQNSLGGN RTA+IC
Sbjct: 265 LAGGARLKEGSHINRSLLTLGTVIRKLSKGKNGHIPYRDSKLTRILQNSLGGNARTAMIC 324

Query: 318 TMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESEMKNIKP 377
           T+SP+RSHVEQSRNTLLFA+CA EV+TNA VNVV+SDKALVKQL+KELA+LES +K+++ 
Sbjct: 325 TISPSRSHVEQSRNTLLFASCANEVATNAQVNVVMSDKALVKQLRKELAKLESRLKSMES 384

Query: 378 LPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIFKRSESTVQ 437
           + V GD+ +LL+EKE+LIE+MDKEIKELT QRDLAQ R+++LL  VG++R+ +  E++  
Sbjct: 385 ISVTGDTAALLREKELLIEKMDKEIKELTWQRDLAQSRVDSLLREVGDNRLSRVGENSA- 444

Query: 438 IVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLKTFD-----TFNGQGQE 497
                 SE+     P  +      T               +++K FD     T   Q Q+
Sbjct: 445 ------SESSEGITPLGLDAGFART---------------NTVKDFDDPSVLTPTRQIQQ 504

Query: 498 ENSPHKVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEVAQRVRANSEDSYKDVQCIEL 557
              P           +D FLL++STP+ + P P+  WE  +Q    N+ED  K+V+CIE 
Sbjct: 505 IPDP-----------EDGFLLNNSTPKFSEPDPFRFWESTSQE---NNEDICKEVRCIET 564

Query: 558 EESNQTPNENVELALARLEDNEGQMISTSRTNQVTSP----QRKNKEIVTINKDYVSGGF 617
           +E++        + L+  +  E ++ +   T++   P    Q ++KE  + N  Y S   
Sbjct: 565 DEASVISKAEDGVLLSSSDRQEKEVATAEETDEGALPSMAMQEEDKES-SHNDSYNSYHA 624

Query: 618 MPNTAEP-KRTLNCIVNFYPAEQSFSSIEAAKDRFQNLKLTRSKSCLTVLMELPPSTWVE 677
           +    +    T++ +  F P E   SS +A       L  +RSKS  +V+M +P + W E
Sbjct: 625 LKQKIQDLHETISLLQQFPPNEAGASSSKA-------LTWSRSKSRRSVVMTIPSALWYE 684

Query: 678 KP-----------ETDKKAQHNGSEINFSGLAEGSRRKRGLSCGNLETEESQSVCNRPSE 737
           K            E D   +  G+E   + L      K G   G      + S C    E
Sbjct: 685 KEEENENILPASFEEDNLERRGGTEQKLAELEPDD--KTGKISGKYSRNSTSSAC---IE 744

Query: 738 DKALEIIEEDDDDNTSVLNFATG----KKGKSKNRLRKRSTHGSRLGRTPKREEAKETTQ 797
           ++ ++ I  D DD T+VL+F  G     K +S+ ++ + ST  + L R   R  +     
Sbjct: 745 EETIKEINIDVDDTTTVLDFVAGVNTIAKPQSEEQI-EASTRTADLWRDAGRGNSAR--- 804

Query: 798 DVGLEEEQDFQSHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDPSDAVYMEV 857
                     Q+ ++ +L+F+  +R IIELW  CNVPLVHRSYFF++FKGDPSD VY+EV
Sbjct: 805 ----------QNRANSILKFERYRRKIIELWARCNVPLVHRSYFFLIFKGDPSDNVYLEV 864

Query: 858 ELRRLFFIREAMSRSTNGFGRNDAITQASSLKTLNREREMLVKRIKKKFSVKERDTLYKK 917
           ELRRL+F+++  +R TN       ++  SSLK+LNRERE L ++++KKF+ +ER  LY +
Sbjct: 865 ELRRLYFLKDTSARGTNTLIDTQIVSLNSSLKSLNREREHLARQLQKKFTKRERVELYVR 924

Query: 918 WGIDLKTKQRSIQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVSREMFGLSFSLQS 966
           WGIDL TKQRS+QL ++LWT TKD  H+ ES+ LVAKL+GFVEP    +EMFGLSF    
Sbjct: 925 WGIDLDTKQRSLQLIRRLWTDTKDLKHMRESSVLVAKLIGFVEPRYAPKEMFGLSFLTPL 934

BLAST of CmaCh12G004270 vs. TrEMBL
Match: A5AQK6_VITVI (Kinesin-like protein OS=Vitis vinifera GN=VIT_03s0038g00700 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 4.8e-237
Identity = 479/970 (49.38%), Postives = 648/970 (66.80%), Query Frame = 1

Query: 11  IQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDRAMYPQSYTFD 70
           +QA+   EE I V +RLRPL+EKE+ +N+ SDW C+N N+++FR++L +R+M+P +Y+FD
Sbjct: 15  MQAATAREEKILVLVRLRPLSEKEIARNEVSDWECINENTVLFRNSLQERSMFPTAYSFD 74

Query: 71  RVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNY 130
           +VF  D TT+QVYEE AKE+ LSVVNGINS+IFAYGQTSSGKTYTM GITEY+VADIY+Y
Sbjct: 75  KVFRGDCTTRQVYEEAAKEIALSVVNGINSSIFAYGQTSSGKTYTMIGITEYTVADIYDY 134

Query: 131 METHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHL 190
           ++ H+ER +VLKFSA+EIYNEAVRDLLS +N+PLRLLDDPE+GT+VEKLTEETL+D +HL
Sbjct: 135 IQNHEERAFVLKFSAMEIYNEAVRDLLSTDNLPLRLLDDPERGTIVEKLTEETLRDWSHL 194

Query: 191 QELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAG 250
           + LLS CE QR+IGET+LNE SSRSHQILRLTIESSAR+F    + ++L A+VNFVDLAG
Sbjct: 195 KNLLSICEAQRQIGETSLNETSSRSHQILRLTIESSAREFLGKGNSTTLAASVNFVDLAG 254

Query: 251 SERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGN 310
           SERASQ MSAG RLKEGCHINRSLL+LGTVIRKLSKGR GHV YRDSKLTRILQ SLGGN
Sbjct: 255 SERASQAMSAGARLKEGCHINRSLLTLGTVIRKLSKGRQGHVNYRDSKLTRILQPSLGGN 314

Query: 311 GRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLES 370
            RTAIICT+SPARSHVEQSRNTLLFA+CAKEV+T A VNVV+SDKALVK LQKELARLES
Sbjct: 315 ARTAIICTLSPARSHVEQSRNTLLFASCAKEVTTKAQVNVVMSDKALVKHLQKELARLES 374

Query: 371 EMKNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIFK 430
           E+++  P     D  +LL++K++ I++M+KEI+ELT+ RD+A+ R+E+LL  +G D+   
Sbjct: 375 ELRSPAPASSTCDHTALLRKKDLQIDKMEKEIRELTKLRDIAESRVEDLLQMIGNDQ--- 434

Query: 431 RSESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLKTFDT--FNG 490
                        S   +    +  S+       D    + D    D  +++F+T  ++G
Sbjct: 435 ------------SSSQWTGIRNDPKSQVGIKWEDDCSVSEADPGCRDIGVRSFNTTQYSG 494

Query: 491 QGQEENSPHKVNPLFSTSHDDDFLLDSSTPELAG-----PYPYNNWEEVAQRVRANSEDS 550
           +G   N+  K + L   S         S+P   G     P P    EE+A     + +D 
Sbjct: 495 RGSGSNTQEKYHQLPQYSEGHSPFDGPSSPISVGNGFVRPDPRCGQEEIALEAGEDPDDL 554

Query: 551 YKDVQCIELEESNQTPNENVELALARLEDNEGQMIS----TSRTNQVTSPQRKNKEIVTI 610
           YK+V+CIE+EES++  ++N++       +NEG  +S     +    +++P +  +E+  I
Sbjct: 555 YKEVRCIEIEESSK--HKNLKSLDTSTGENEGMAVSGNGDVTDGEIISAPTKGEREVSHI 614

Query: 611 NKDYVSGGFMPNTAEPKRTLNCIVNFYPAEQSFSSIEAAKDRFQNLKLTRSKSCLTVLME 670
              +  G       + ++T+  +V+ YP E S  +++A     ++L LTRS SC   LM 
Sbjct: 615 QNGFTYGALEQKIQDVQKTIESLVSPYPDEPSPWALDADTPSSRSLTLTRSWSCRANLM- 674

Query: 671 LPPSTWVEKPETDKKAQHNGSEINFSGLAEGSRRKR-----GLSCGNLETEESQSVCNRP 730
              S+  EK E       +G E +F G  E  RR+      G +   L   +SQS     
Sbjct: 675 TGSSSPCEKVEQRLSTPPSGFEKDFPGRPESFRRRHPPLNYGANMPRLSRTDSQSSFGSA 734

Query: 731 SEDKALEIIEEDDDDNTSVLNFATGKKGKSKNRLRKRSTHGSRLGRTPKREEAKETTQDV 790
             D+        D+D TS+  F  G K  +K +  K+   G       + ++ ++  +DV
Sbjct: 735 FVDELKAEKTSADEDITSIQTFVAGLKEMAKLQYEKQLVDGQVEETGTRADKLEKNVKDV 794

Query: 791 GLEEEQDFQSHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDPSDAVYMEVEL 850
           GL+  Q+  +  DW LEF+ QQR+IIELW  CNV L+HR+YFF+LF+GDP D++YMEVEL
Sbjct: 795 GLDPMQE-GTLPDWPLEFERQQREIIELWQTCNVSLIHRTYFFLLFRGDPMDSIYMEVEL 854

Query: 851 RRLFFIREAMSRSTNGFGRNDAITQASSLKTLNREREMLVKRIKKKFSVKERDTLYKKWG 910
           RRL F++E  S+          +TQASS++ L RERE L K + K+FS  ER+ L++KWG
Sbjct: 855 RRLSFLKETFSQGNQSLEDGRTLTQASSIRALRRERETLSKLMHKRFSEGERNRLFQKWG 914

Query: 911 IDLKTKQRSIQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVSREMFGLSFSLQSLD 965
           I L +K+R +QLA++LW+ T D  H++ESAA+VAKL+ FVE  Q  +EMFGLSF+     
Sbjct: 915 IKLDSKRRRLQLAQRLWSNTTDMSHVNESAAIVAKLIKFVEQGQALKEMFGLSFTPHRTR 965

BLAST of CmaCh12G004270 vs. TrEMBL
Match: B9GRG4_POPTR (Kinesin-like protein OS=Populus trichocarpa GN=POPTR_0002s02910g PE=3 SV=2)

HSP 1 Score: 823.2 bits (2125), Expect = 3.4e-235
Identity = 484/962 (50.31%), Postives = 644/962 (66.94%), Query Frame = 1

Query: 14  SNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDRAMYPQSYTFDRVF 73
           ++G EE I VS+RLRPLNE+E+ +ND SDW C+NN++IIF+S +P+R+M+P +YTFDRVF
Sbjct: 17  TSGQEERILVSVRLRPLNEREIVRNDVSDWECINNSTIIFKSNMPERSMFPTAYTFDRVF 76

Query: 74  GCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYMET 133
           G D  TKQVYEEG KE+ L  V+G+NSTIFAYGQTSSGKTYTM GITEY++ADIY YM+ 
Sbjct: 77  GSDCLTKQVYEEGVKEIALLAVSGVNSTIFAYGQTSSGKTYTMRGITEYALADIYEYMKQ 136

Query: 134 HQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHLQEL 193
           H+ERE+VLKFSA+EIYNEAVRDLLS  + PLRLLDDPE+GTVVE L E+TL D NHL EL
Sbjct: 137 HKEREFVLKFSAMEIYNEAVRDLLSTSSSPLRLLDDPERGTVVEGLIEDTLVDWNHLLEL 196

Query: 194 LSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGSER 253
           +S CE QR+IGETTLNE SSRSHQILRLT+ESSAR +  + + ++LTATVNFVDLAGSER
Sbjct: 197 ISICEDQRQIGETTLNETSSRSHQILRLTVESSARGYLGAGNSTTLTATVNFVDLAGSER 256

Query: 254 ASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNGRT 313
           ASQT++  TRLKEG HINRSLL+LGTVIRKLSKGR GH+PYRDSKLTRILQNSLGGN RT
Sbjct: 257 ASQTLAVVTRLKEGSHINRSLLTLGTVIRKLSKGRNGHIPYRDSKLTRILQNSLGGNART 316

Query: 314 AIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESEMK 373
           AIICT+SPARSHVEQSRNTLLFA+CAKEV+TNA VN+V+SDK LVK+LQKE+ARLE+E+K
Sbjct: 317 AIICTLSPARSHVEQSRNTLLFASCAKEVTTNAQVNLVMSDKTLVKKLQKEVARLENELK 376

Query: 374 NIKPLPVKGDSNSLLKEKEILIE---QMDKEIKELTRQRDLAQYRIENLLHSVG-EDRIF 433
           +       GDS +LL+EKE+ IE   +M +EI+ELT QRD A+  +++LL S G +D++F
Sbjct: 377 SAGSNSFVGDSTALLREKELQIEKVRKMHQEIQELTWQRDQAESHLQSLLESFGIKDQVF 436

Query: 434 KRSESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLKTFDTFNGQ 493
           +                   + P + SE + A       LD DL       KTF  F+  
Sbjct: 437 RMD---------------GHSAPGS-SEMINA-----FRLDADL----PGTKTFKDFDYP 496

Query: 494 GQEENSPHKVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEVAQRVRANSEDSYKDVQC 553
           G    SP+K         +D+FLLD STP+ +GP      E++ +R   +++D  ++V  
Sbjct: 497 GAV--SPNK---QIIQIPEDNFLLDGSTPKFSGP----GCEDIGKRNSEDAKDICEEVPG 556

Query: 554 IELEESNQTPNENVELALARLEDNEGQMISTSRTNQVTSPQRKNKEIVTINKDYVSGGFM 613
             + ES +   +  ++    LE+            +V S Q ++KE++ IN +       
Sbjct: 557 TIMVESRRKVKKVADILFPVLEEK-----IPMHEGEVPSSQEEDKELIHINSNNTHETVK 616

Query: 614 PNTAEPKRTLNCIVNFYPAEQSFSSIEAAKDRFQNLKLTRSKSCLTVLMELPPSTWVEKP 673
               E   T++ +      E+S S  +A +  ++NLK TRSKS  +VLM +P + W EK 
Sbjct: 617 QKIQELHETIDHL------ERSPSFSDAVESSYKNLKWTRSKSRRSVLMTIPYALWSEKE 676

Query: 674 ETDKKAQHNGSEINFSGLAEGSRRK-----RGLSCGNLETEESQS-VCNRPSEDKALEII 733
           E + +     SE +F   +E  ++K       +  GN   E+SQ+ +    +E+  ++ I
Sbjct: 677 EDNGRVSPTASEKDFPERSEDLKQKLLELEHDVKLGNKSQEDSQNPLYGASAEEDVIKDI 736

Query: 734 EEDDDDNTSVLNFATGKKGKSKNRLRKRSTHGSRLGRTPKREEAKETTQDVGLEEEQDFQ 793
           + D +D TSVL+F  G                  + +   +  ++  ++D+ + +     
Sbjct: 737 DVDVEDTTSVLDFVAG------------------VNKMAAKLHSENLSRDMQVLQASTRH 796

Query: 794 SHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDPSDAVYMEVELRRLFFIREA 853
            HS+W ++++  +R IIELW  CNVPLVHRSYFF+LFKGDPSD VYMEVELRRL+F+++ 
Sbjct: 797 HHSNWQVKYERYRRKIIELWFRCNVPLVHRSYFFLLFKGDPSDNVYMEVELRRLYFLKDT 856

Query: 854 MSRSTNGFGRNDAITQASSLKTLNREREMLVKRIKKKFSVKERDTLYKKWGIDLKTKQRS 913
            S   N        + ASSLK L+RER+ML K+++KK++  ER+ LY+KWGI L TKQRS
Sbjct: 857 FSSGANTIIDGKIASPASSLKALSRERDMLAKQLQKKYTKTERERLYQKWGIPLDTKQRS 912

Query: 914 IQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVSREMFGLSFSLQSLDQRSFPWKRN 966
           +QLA++LWT  +D  HI +SA LVAKL G VEP    +EMFGLSF   S +Q+   W+ N
Sbjct: 917 LQLARRLWTDVRDMRHIKDSATLVAKLAGIVEPRHAPKEMFGLSF---STNQKPPSWRDN 912

BLAST of CmaCh12G004270 vs. TrEMBL
Match: D7SP24_VITVI (Kinesin-like protein OS=Vitis vinifera GN=VIT_04s0023g03060 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 3.8e-234
Identity = 487/990 (49.19%), Postives = 657/990 (66.36%), Query Frame = 1

Query: 10  VIQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLP--DRAMYPQSY 69
           V++  +G  E + VS+RLRPLNEKE+++ND+ DW C+N+ +IIF++ LP  +R+MYP +Y
Sbjct: 9   VMRGPSGRGERVVVSVRLRPLNEKEISRNDALDWECINDTTIIFKNHLPIPERSMYPSAY 68

Query: 70  TFDRVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADI 129
           TFDRVF  DSTT++VYE GAKEV LSVV+GINS+IFAYGQTSSGKT+TM+GITEY++ADI
Sbjct: 69  TFDRVFRSDSTTREVYEAGAKEVALSVVSGINSSIFAYGQTSSGKTFTMSGITEYTMADI 128

Query: 130 YNYMETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDR 189
           Y+++E H+ERE++LKFSA+EIYNE+VRDLLS +  PLRLLDDPE+GT+VEKLTEETL+D 
Sbjct: 129 YDHIERHKEREFLLKFSAMEIYNESVRDLLSSDTAPLRLLDDPERGTIVEKLTEETLRDW 188

Query: 190 NHLQELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVD 249
           NHL ELLS CE QR+IGET LNE SSRSHQILRLT+ESSAR+F  +++ S LT+TVNFVD
Sbjct: 189 NHLIELLSLCEAQRQIGETALNETSSRSHQILRLTVESSAREFLGNDNSSVLTSTVNFVD 248

Query: 250 LAGSERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSL 309
           LAGSERASQ++SAGTRLKEGCHINRSLL+LGTVIRKLSKGR+GH+PYRDSKLTRILQ+SL
Sbjct: 249 LAGSERASQSLSAGTRLKEGCHINRSLLTLGTVIRKLSKGRSGHIPYRDSKLTRILQSSL 308

Query: 310 GGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELAR 369
           GGN +TAIICTMSPARSHVEQSRNTLLFA+CAKEV+TNA VNVV+SDKALVK LQ+ELAR
Sbjct: 309 GGNAKTAIICTMSPARSHVEQSRNTLLFASCAKEVTTNAQVNVVMSDKALVKHLQRELAR 368

Query: 370 LESEMKNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDR 429
           LE+ +++ +P  +  D+ +LL++K++ IE+++KE++ELT QRDLAQ ++E+LL  VG+DR
Sbjct: 369 LENSLRSPEPTSICLDTATLLRDKDLQIEKLEKELRELTLQRDLAQSQVEDLLGVVGDDR 428

Query: 430 I------FKRSESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLK 489
           +             +++     SE  +        +  PA+      L  D ++ D  L+
Sbjct: 429 LPMIWADMDDHYPKLRVRRSWESENPTSETFALADDQTPASGLRTFAL-ADDQTPDVGLR 488

Query: 490 TFDT---FNGQGQEENSPHKVNPLFSTSHDD------DFLLDSSTPELAGPYPYNNWEEV 549
           T DT    +G   +++  H   P    S D+        L+  +TP        + W+++
Sbjct: 489 TCDTSQYSDGNSVDDSDDH--YPPLPESEDNFLHNGTSALVSVNTPNHVAIDLSSQWDKI 548

Query: 550 AQRVRANSEDSYKDVQCIELEESNQTPNENVELALARLEDNEGQMISTSRTNQVTSPQRK 609
            ++  ANSED  K+V+CIE+E S                D E   +S  R       +  
Sbjct: 549 EEQSNANSEDLCKEVRCIEIEHS------------IMKRDIESNTLSPVRDTDALELK-- 608

Query: 610 NKEIVTINKDYVSGGFMPNTAEPKRTLNCIVN--FYPAEQSFSS--IEAAKDRFQNLKLT 669
               V  N D  +  F     +  + LNC       P+ Q FS   +E      ++LKLT
Sbjct: 609 ----VVRNGDGANQEFTSPLLKEDKELNCNQRTVVIPSPQEFSPWLLEKENSSCRSLKLT 668

Query: 670 RSKSCLTVLMELPPSTWVEKPETDKKAQHNGSEINFSGLAEGSRRKRGLSCGNLETE--- 729
           RS+SC    M    S W EK E DK    N  E +F G  EG ++K  L+  N +TE   
Sbjct: 669 RSRSCKASFMYCSSSPWFEKEEKDKYTPSNVFEKDFIGRPEGFQKK--LASLNYDTEIDK 728

Query: 730 ----ESQSVCNRPSEDKALE--IIEEDDDDNTSVLNFATGKKGKSKNRLRKRSTHGSRLG 789
                 Q+     S D+  E  +    D+D TS+  +  G K  +K +  +R        
Sbjct: 729 LSRKGGQTFRGSSSVDQLKEQVVTTSTDEDVTSLNTYVAGLKEMAKFQYEERLADDQE-- 788

Query: 790 RTPKREEAKETTQDVGLEE-EQDFQSHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFI 849
             P+  ++ +  +DVGL+  + D  S S W  EF+  Q++IIELW +CNV LVHR+YFF+
Sbjct: 789 SEPEANKSVKNVKDVGLDPIQDDLASPSRWPFEFKRLQKEIIELWHSCNVSLVHRTYFFL 848

Query: 850 LFKGDPSDAVYMEVELRRLFFIREAMSRSTNGFGRNDAITQASSLKTLNREREMLVKRIK 909
           LF+GDP+D++YMEVELRRL F+++  SR         A+T ASS++ L REREML K+++
Sbjct: 849 LFQGDPADSIYMEVELRRLSFLKDTFSRGNQTVVDGHALTPASSVRALRREREMLCKQMQ 908

Query: 910 KKFSVKERDTLYKKWGIDLKTKQRSIQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQ 969
           KK S  ER +L+ KWG+ L  K R +QLA +LWT T+D +HI ESA +VA+L  FV+P +
Sbjct: 909 KKLSEDERMSLFLKWGVQLNAKNRRLQLAYRLWTDTEDMNHISESANIVARLTRFVQPEE 968

BLAST of CmaCh12G004270 vs. TAIR10
Match: AT3G51150.2 (AT3G51150.2 ATP binding microtubule motor family protein)

HSP 1 Score: 547.7 bits (1410), Expect = 1.4e-155
Identity = 277/417 (66.43%), Postives = 357/417 (85.61%), Query Frame = 1

Query: 11  IQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRS--TLPDRAMYPQSYT 70
           +Q S+G EE I VS+RLRPLN +E  +ND +DW C+N+ ++I+RS  ++ +R+MYP +YT
Sbjct: 8   MQGSSGREEKIFVSVRLRPLNVRERARNDVADWECINDETVIYRSHLSISERSMYPTAYT 67

Query: 71  FDRVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIY 130
           FDRVFG + +T++VY++GAKEV LSVV+G+++++FAYGQTSSGKTYTM GIT+Y++ADIY
Sbjct: 68  FDRVFGPECSTREVYDQGAKEVALSVVSGVHASVFAYGQTSSGKTYTMIGITDYALADIY 127

Query: 131 NYMETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRN 190
           +Y+E H ERE++LKFSA+EIYNE+VRDLLS +  PLR+LDDPEKGTVVEKLTEETL+D N
Sbjct: 128 DYIEKHNEREFILKFSAMEIYNESVRDLLSTDISPLRVLDDPEKGTVVEKLTEETLRDWN 187

Query: 191 HLQELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDL 250
           H +ELLS C  QR+IGET LNE SSRSHQILRLT+ES+AR++   +  S+LTATVNF+DL
Sbjct: 188 HFKELLSICIAQRQIGETALNEVSSRSHQILRLTVESTAREYLAKDKFSTLTATVNFIDL 247

Query: 251 AGSERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLG 310
           AGSERASQ++SAGTRLKEG HINRSLL+LGTVIRKLSKG+ GH+P+RDSKLTRILQ SLG
Sbjct: 248 AGSERASQSLSAGTRLKEGGHINRSLLTLGTVIRKLSKGKNGHIPFRDSKLTRILQTSLG 307

Query: 311 GNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARL 370
           GN RT+IICT+SPAR HVEQSRNTLLFA+CAKEV+TNA VNVV+SDKALV+ LQ+ELA+L
Sbjct: 308 GNARTSIICTLSPARVHVEQSRNTLLFASCAKEVTTNAQVNVVMSDKALVRHLQRELAKL 367

Query: 371 ESEMKNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGE 426
           ESE+ + +   V  D+ +LLKEK++ IE+++KE+ +L ++ + A  RIE+L   +GE
Sbjct: 368 ESELSSPRQALVVSDTTALLKEKDLQIEKLNKEVFQLAQELERAYSRIEDLQQIIGE 424

BLAST of CmaCh12G004270 vs. TAIR10
Match: AT5G66310.1 (AT5G66310.1 ATP binding microtubule motor family protein)

HSP 1 Score: 545.8 bits (1405), Expect = 5.3e-155
Identity = 280/441 (63.49%), Postives = 362/441 (82.09%), Query Frame = 1

Query: 15  NGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRS--TLPDRAMYPQSYTFDRV 74
           +G +E I VS+R+RPLN+KE  +ND  DW C+NN +II+RS  ++ +R+MYP +YTFDRV
Sbjct: 13  SGTQEKIYVSVRMRPLNDKEKFRNDVPDWECINNTTIIYRSHLSISERSMYPSAYTFDRV 72

Query: 75  FGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYME 134
           F  +  T+QVYE+GAKEV  SVV+G+N+++FAYGQTSSGKTYTM+GIT+ ++ DIY Y++
Sbjct: 73  FSPECCTRQVYEQGAKEVAFSVVSGVNASVFAYGQTSSGKTYTMSGITDCALVDIYGYID 132

Query: 135 THQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHLQE 194
            H+ERE++LKFSA+EIYNE+VRDLLS +  PLRLLDDPEKGTVVEKLTEETL+D NH +E
Sbjct: 133 KHKEREFILKFSAMEIYNESVRDLLSTDTSPLRLLDDPEKGTVVEKLTEETLRDWNHFKE 192

Query: 195 LLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGSE 254
           LLS C+ QR+IGET LNE SSRSHQILRLT+ES AR+F  ++  S+LTATVNF+DLAGSE
Sbjct: 193 LLSVCKAQRQIGETALNEVSSRSHQILRLTVESIAREFSTNDKFSTLTATVNFIDLAGSE 252

Query: 255 RASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNGR 314
           RASQ++SAGTRLKEGCHINRSLL+LGTVIRKLSK +TGH+P+RDSKLTRILQ+SLGGN R
Sbjct: 253 RASQSLSAGTRLKEGCHINRSLLTLGTVIRKLSKEKTGHIPFRDSKLTRILQSSLGGNAR 312

Query: 315 TAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESEM 374
           TAIICTMSPAR HVEQSRNTLLFA+CAKEV+TNA VNVV+SDKALVK LQ+ELA+LESE+
Sbjct: 313 TAIICTMSPARIHVEQSRNTLLFASCAKEVTTNAQVNVVMSDKALVKHLQRELAKLESEL 372

Query: 375 KNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIFKRS 434
           ++     +  D+ +LL EK++ +E++ KE+ +L +Q + A+  I++L   V E++   + 
Sbjct: 373 RSPSQASIVSDTTALLTEKDLEVEKLKKEVFQLAQQLEQARSEIKDLRRMVEEEK--NQE 432

Query: 435 ESTVQIVPETMSETMSETVPE 454
           + T+    E ++  M    P+
Sbjct: 433 KETLSTETEGLNVLMEHQYPK 451

BLAST of CmaCh12G004270 vs. TAIR10
Match: AT2G21300.1 (AT2G21300.1 ATP binding microtubule motor family protein)

HSP 1 Score: 538.5 bits (1386), Expect = 8.5e-153
Identity = 299/547 (54.66%), Postives = 389/547 (71.12%), Query Frame = 1

Query: 18  EETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDRAMYPQSYTFDRVFGCDS 77
           EE I V +RLRPLNEKE+  N+++DW C+N+ ++++R+TL + + +P +Y+FDRV+  + 
Sbjct: 22  EEKILVLVRLRPLNEKEILANEAADWECINDTTVLYRNTLREGSTFPSAYSFDRVYRGEC 81

Query: 78  TTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYMETHQER 137
            T+QVYE+G KEV LSVV GINS+IFAYGQTSSGKTYTM+GITE++VADI++Y+  H++R
Sbjct: 82  PTRQVYEDGPKEVALSVVKGINSSIFAYGQTSSGKTYTMSGITEFAVADIFDYIFKHEDR 141

Query: 138 EYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHLQELLSFC 197
            +V+KFSAIEIYNEA+RDLLS ++ PLRL DDPEKG  VEK TEETL+D NHL+EL+S C
Sbjct: 142 AFVVKFSAIEIYNEAIRDLLSPDSTPLRLRDDPEKGAAVEKATEETLRDWNHLKELISVC 201

Query: 198 EVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGSERASQT 257
           E QRKIGET+LNE SSRSHQI++LT+ESSAR+F   E+ ++L A+VNF+DLAGSERASQ 
Sbjct: 202 EAQRKIGETSLNERSSRSHQIIKLTVESSAREFLGKENSTTLMASVNFIDLAGSERASQA 261

Query: 258 MSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNGRTAIIC 317
           +SAG RLKEGCHINRSLL+LGTVIRKLS GR GH+ YRDSKLTRILQ  LGGN RTAI+C
Sbjct: 262 LSAGARLKEGCHINRSLLTLGTVIRKLSNGRQGHINYRDSKLTRILQPCLGGNARTAIVC 321

Query: 318 TMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESEMKNIKP 377
           T+SPARSHVEQ+RNTLLFA CAKEV+T A +NVV+SDKALVKQLQ+ELARLESE++N  P
Sbjct: 322 TLSPARSHVEQTRNTLLFACCAKEVTTKAQINVVMSDKALVKQLQRELARLESELRNPAP 381

Query: 378 LPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIFKRSESTVQ 437
                D    L++K++ I++M+K++ E+T+QRD+AQ R+E+ +  V  D   K       
Sbjct: 382 ATSSCDCGVTLRKKDLQIQKMEKQLAEMTKQRDIAQSRLEDFMKMVEHDASSKAG----- 441

Query: 438 IVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLKTFDTFNGQGQEENSPH 497
             P   + T ++    ++SE      PD         SD +S               +P 
Sbjct: 442 -TPHFRNRT-NKWEDGSVSEISGVVDPDRT----SFISDGTS---------------TPL 501

Query: 498 KVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEVAQRVRANSEDSYKDVQCIELEESNQ 557
                   SH DD L +  +P  +G                 SE+  K+VQCIE+EES +
Sbjct: 502 STARAHVRSHSDDDLEEEMSPRHSGD---------------QSEEYCKEVQCIEMEESTR 527

Query: 558 TPNENVE 565
             N + E
Sbjct: 562 DINNDSE 527

BLAST of CmaCh12G004270 vs. TAIR10
Match: AT4G24170.1 (AT4G24170.1 ATP binding microtubule motor family protein)

HSP 1 Score: 533.9 bits (1374), Expect = 2.1e-151
Identity = 347/730 (47.53%), Postives = 473/730 (64.79%), Query Frame = 1

Query: 16  GLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFR-STLPDRAMYPQSYTFDRVFG 75
           G EE I VS+R+RPLNEKE T+ND  DW C+N+ +II +   LPD++    SYTFD+VFG
Sbjct: 2   GGEEKILVSVRVRPLNEKEKTRNDRCDWECINDTTIICKFHNLPDKS----SYTFDKVFG 61

Query: 76  CDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYMETH 135
            +  TKQVY++GAKEV L V++GINS+IFAYGQTSSGKTYTM+GITE+++ DI+ Y++ H
Sbjct: 62  FECPTKQVYDDGAKEVALCVLSGINSSIFAYGQTSSGKTYTMSGITEFAMDDIFAYIDKH 121

Query: 136 -QEREYVLKFSAIEIYNEAVRDLLSFEN-VPLRLLDDPEKGTVVEKLTEETLKDRNHLQE 195
            QER++ LKFSA+EIYNEAVRDLL  ++  PLRLLDDPE+GTVVEKL EETL+DR+HL+E
Sbjct: 122 KQERKFTLKFSAMEIYNEAVRDLLCEDSSTPLRLLDDPERGTVVEKLREETLRDRSHLEE 181

Query: 196 LLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGSE 255
           LLS CE QRKIGET+LNE SSRSHQILRLTIESS+++F   ES ++L A+V FVDLAGSE
Sbjct: 182 LLSICETQRKIGETSLNEISSRSHQILRLTIESSSQQFSP-ESSATLAASVCFVDLAGSE 241

Query: 256 RASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNGR 315
           RASQT+SAG+RLKEGCHINRSLL+LGTVIRKLSKG+ GH+PYRDSKLTRILQNSLGGN R
Sbjct: 242 RASQTLSAGSRLKEGCHINRSLLTLGTVIRKLSKGKNGHIPYRDSKLTRILQNSLGGNAR 301

Query: 316 TAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESEM 375
           TAIICTMSPARSH+EQSRNTLLFATCAKEV+TNA VN+VVS+KALVKQLQ+ELAR+E+E+
Sbjct: 302 TAIICTMSPARSHLEQSRNTLLFATCAKEVTTNAQVNLVVSEKALVKQLQRELARMENEL 361

Query: 376 KNIKPLPVKGDSN---SLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIF 435
           KN+ P      S+    +LK+KE LI +M+++I EL  QRD+AQ R+ENLL S  E+   
Sbjct: 362 KNLGPASASSTSDFYALMLKQKEELIAKMEEQIHELKWQRDVAQSRVENLLKSTAEE--- 421

Query: 436 KRSESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLKTFDTFNGQ 495
            RS S+      +M         ++     P  + +L + +L    +D  L    T    
Sbjct: 422 -RSSSS------SMDSRRRRISYDSTDFDEPRMLNNLGKSNLYSPDEDGFLLDDTTPQFP 481

Query: 496 GQEENSPHKVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEVAQRVRANSEDSYKD-VQ 555
           G   +   +     +T   +D   +    E+      N+ E  A+RV+   +DS  D V+
Sbjct: 482 GHNLHDKWEEMAQSTTQEPEDACKEVRCIEV------NSGE--AERVQI--QDSLDDIVE 541

Query: 556 CIELEESNQTPNENVELALARLEDNEGQMISTSRTNQVTSPQRKNKEIVTINKDYVSGGF 615
             E E++ ++  ++ + ++  + D E  + +        S ++  +++    +       
Sbjct: 542 KKEYEQNYESQKDDADSSIKNI-DMELSLYTKPEAEDGVSVKKLIEDVQETEQSVEKQKQ 601

Query: 616 MPNTAEPKRTLN-----CIVNFYPAEQSFSSIEAAKDRFQNLKLTRSKSCLTVLMELPPS 675
            P   E ++ L+      +    P E+       A D+ +      ++  LT+      +
Sbjct: 602 SPKKEEMEQYLSRDMSEQVTKSLPEEEQCVQEYGAYDKLE------AQDVLTI------N 661

Query: 676 TWVEKPETDKKAQHNGSEINFSGLAEGSRRKRGLSCGNLETEESQSVCNRPSED-KALEI 732
              E  +T++  +   ++ N S   E  ++       NL  ++S+ +   P ED K +E+
Sbjct: 662 KLEESQQTEQSVEKEDTKKNLSSKKEDLKQ-------NLSMDQSEQLYKSPPEDEKCVEV 686

BLAST of CmaCh12G004270 vs. TAIR10
Match: AT4G38950.1 (AT4G38950.1 ATP binding microtubule motor family protein)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-149
Identity = 297/539 (55.10%), Postives = 390/539 (72.36%), Query Frame = 1

Query: 18  EETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDRAMYPQSYTFDRVFGCDS 77
           EE I V +RLRPLN+KE+  N+++DW C+N+ +I++R+TL + + +P +Y+FD+V+  + 
Sbjct: 11  EEKILVLVRLRPLNQKEIAANEAADWECINDTTILYRNTLREGSNFPSAYSFDKVYRGEC 70

Query: 78  TTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYMETHQER 137
            T+QVYE+G KE+ LSVV GIN +IFAYGQTSSGKTYTM GITE++VADI++Y+  H+ER
Sbjct: 71  PTRQVYEDGTKEIALSVVKGINCSIFAYGQTSSGKTYTMTGITEFAVADIFDYIFQHEER 130

Query: 138 EYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHLQELLSFC 197
            + +KFSAIEIYNEA+RDLLS +   LRL DDPEKGTVVEK TEETL+D NHL+ELLS C
Sbjct: 131 AFSVKFSAIEIYNEAIRDLLSSDGTSLRLRDDPEKGTVVEKATEETLRDWNHLKELLSIC 190

Query: 198 EVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGSERASQT 257
           E QRKIGET+LNE SSRSHQ++RLT+ESSAR+F   E+ ++L A+VNF+DLAGSERASQ 
Sbjct: 191 EAQRKIGETSLNERSSRSHQMIRLTVESSAREFLGKENSTTLMASVNFIDLAGSERASQA 250

Query: 258 MSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNGRTAIIC 317
           MSAGTRLKEGCHINRSLL+LGTVIRKLSKGR GH+ +RDSKLTRILQ  LGGN RTAIIC
Sbjct: 251 MSAGTRLKEGCHINRSLLTLGTVIRKLSKGRQGHINFRDSKLTRILQPCLGGNARTAIIC 310

Query: 318 TMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESEMKNIKP 377
           T+SPARSHVE ++NTLLFA CAKEV+T A +NVV+SDKAL+KQLQ+ELARLE+E++N   
Sbjct: 311 TLSPARSHVELTKNTLLFACCAKEVTTKARINVVMSDKALLKQLQRELARLETELRNPAS 370

Query: 378 LPVKG-DSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIFKRSESTV 437
            P    D    +++K++ I++M+KEI EL +QRDLAQ R+E+ +      R+ + + ++ 
Sbjct: 371 SPASNCDCAMTVRKKDLQIQKMEKEIAELRKQRDLAQSRLEDFM------RMIEHNVASK 430

Query: 438 QIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLKTFDTFNGQGQEENSP 497
              P   + T  +    ++SET          +D D RS          F   G   ++P
Sbjct: 431 PGTPHFGNHT-DKWEDGSVSETSGV-------VDSDRRS----------FISDGM--STP 490

Query: 498 HKVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEVAQRVRANSEDSYKDVQCIELEES 556
             ++  +  SH DD  LD               E++ +R    SE+  ++VQCIE EES
Sbjct: 491 LSISRAYVHSHSDDDDLD---------------EDLPRRSEDLSEEYCREVQCIETEES 508

BLAST of CmaCh12G004270 vs. NCBI nr
Match: gi|659088053|ref|XP_008444776.1| (PREDICTED: kinesin-like protein NACK1 [Cucumis melo])

HSP 1 Score: 1466.1 bits (3794), Expect = 0.0e+00
Identity = 773/980 (78.88%), Postives = 855/980 (87.24%), Query Frame = 1

Query: 1   MEAKEPIQGVIQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDR 60
           M  +E I+GVI  SNGLEETIRVSIRLRPLNEKEL KNDSSDW CLN+NS++FRSTLP+R
Sbjct: 1   MGGEELIKGVINNSNGLEETIRVSIRLRPLNEKELMKNDSSDWECLNSNSVMFRSTLPER 60

Query: 61  AMYPQSYTFDRVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGIT 120
           +++P SYTFDRVFG DSTTKQVYEEGAKEV LSVVNGINSTIFAYGQTSSGKT+TMNG+T
Sbjct: 61  SLFPHSYTFDRVFGIDSTTKQVYEEGAKEVALSVVNGINSTIFAYGQTSSGKTFTMNGVT 120

Query: 121 EYSVADIYNYMETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLT 180
           +YSVADIY+Y+E+HQ+RE+VLKFSAIEIYNEAV+DLLS ENVPLRLLDDPEKGTVVEKLT
Sbjct: 121 QYSVADIYSYIESHQDREFVLKFSAIEIYNEAVKDLLSLENVPLRLLDDPEKGTVVEKLT 180

Query: 181 EETLKDRNHLQELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLT 240
           EE LKDRNHLQEL+SFCEVQRKIGET+LNE SSRSHQILRLTIESSARKFKKSES S+LT
Sbjct: 181 EEILKDRNHLQELISFCEVQRKIGETSLNETSSRSHQILRLTIESSARKFKKSESSSTLT 240

Query: 241 ATVNFVDLAGSERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLT 300
           ATVNFVDLAGSERASQT S GTRLKEGCHINRSLL+LGTVIRKLSKGR GH+PYRDSKLT
Sbjct: 241 ATVNFVDLAGSERASQTNSGGTRLKEGCHINRSLLTLGTVIRKLSKGRNGHIPYRDSKLT 300

Query: 301 RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ 360
           RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ
Sbjct: 301 RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ 360

Query: 361 LQKELARLESEMKNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLL 420
           LQKELARLESEMKN+KPLP+KGDS SLLKEKE++IEQMDK+IKELTRQRDLAQYRIENLL
Sbjct: 361 LQKELARLESEMKNLKPLPLKGDSTSLLKEKELVIEQMDKQIKELTRQRDLAQYRIENLL 420

Query: 421 HSVGEDRIFKRSESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSS- 480
           HSVGEDRIFK SESTV                         T+PDLV+LDLDLRSDDSS 
Sbjct: 421 HSVGEDRIFKLSESTVN------------------------TIPDLVDLDLDLRSDDSSF 480

Query: 481 -------LKTFDTFNGQGQEENSPHKVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEV 540
                  LKTFDTF  Q  EENSPHK++PLF+ +H+DDFLLDSSTPELAGP PY +WEE+
Sbjct: 481 KAFDESSLKTFDTFTAQ--EENSPHKIDPLFTMNHEDDFLLDSSTPELAGPDPYQDWEEI 540

Query: 541 AQRVRANSEDSYKDVQCIELEESNQTPNENVELALARLEDNEGQMISTSRTNQVTSPQRK 600
           A+RV ANSED  KDVQCIELEES ++ NEN +L LA LEDNE QMIS S  N  TSPQRK
Sbjct: 541 AERVHANSEDGCKDVQCIELEESKESVNENGDLTLATLEDNERQMIS-SFDNPETSPQRK 600

Query: 601 NKEIVTINKDYVSGGFMPNTAEPKRTLNCIVNFYPAEQSFSSIEAAKDRFQNLKLTRSKS 660
           NKEI+ INK +   G +   +E ++TLNCIVN YP+EQSFSSIEAAK   Q LKL RSKS
Sbjct: 601 NKEIIPINKGHTYDGLIQKASEMEKTLNCIVNLYPSEQSFSSIEAAK---QKLKLARSKS 660

Query: 661 CLTVLMELPPSTWVEKPETDKKAQHNGSEINFSGLAEGSRRKRGLSCG----NLETEESQ 720
           CLTVLM +PPST +EK E DKK +  GS++NFSG AEGSRR+RGLSCG    NL+ ++SQ
Sbjct: 661 CLTVLMTIPPSTLIEKVEDDKKTRSIGSDVNFSGKAEGSRRRRGLSCGILEANLDFKDSQ 720

Query: 721 SVCNRPSEDKALEIIEEDDDDNTSVLNFATGKKGKSKNRLRKRSTHGSRLGRTPKREEAK 780
           SVC+R S+ K L+II+EDDDDNTSVLNFATGK+GKSKNR++KRS  GSRLGR  K+EE K
Sbjct: 721 SVCSRCSDTKTLQIIDEDDDDNTSVLNFATGKRGKSKNRIKKRS--GSRLGRVSKKEEPK 780

Query: 781 ETTQDVGLEEEQDFQSHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDPSDAV 840
           ETTQ+V  EEE++ Q+HS+W+LEF+GQQRDIIELWDACNVPLVHRSYFFILFKGDPSDAV
Sbjct: 781 ETTQEVHTEEEKELQAHSEWMLEFEGQQRDIIELWDACNVPLVHRSYFFILFKGDPSDAV 840

Query: 841 YMEVELRRLFFIREAMSRSTNGFGRNDAITQASSLKTLNREREMLVKRIKKKFSVKERDT 900
           YMEVELRRLFFIREA+SRS NG GR D +TQ SSLK LNREREML +R+KKKFS KERD 
Sbjct: 841 YMEVELRRLFFIREAISRSANGSGRGDTVTQTSSLKALNREREMLARRMKKKFSAKERDA 900

Query: 901 LYKKWGIDLKTKQRSIQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVSREMFGLSF 960
           LY KWGIDLKTKQRSIQ+A+ LW+RTKDFDHI+ESAALVAKL+GFVEP+QVSREMFGLSF
Sbjct: 901 LYVKWGIDLKTKQRSIQVARMLWSRTKDFDHINESAALVAKLIGFVEPNQVSREMFGLSF 948

Query: 961 SLQSLDQRSFPWKRNMSLPF 969
           SLQSLD RSFPWKRNMSLPF
Sbjct: 961 SLQSLDHRSFPWKRNMSLPF 948

BLAST of CmaCh12G004270 vs. NCBI nr
Match: gi|449470116|ref|XP_004152764.1| (PREDICTED: kinesin-like protein NACK2 [Cucumis sativus])

HSP 1 Score: 1455.7 bits (3767), Expect = 0.0e+00
Identity = 764/984 (77.64%), Postives = 853/984 (86.69%), Query Frame = 1

Query: 1   MEAKEPIQGVIQASNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDR 60
           M  +E I+GVI  SNGLEETIRVSIRLRPLNEKEL KNDSSDW C+N+ S++FRSTLP+R
Sbjct: 1   MGGEELIKGVINNSNGLEETIRVSIRLRPLNEKELMKNDSSDWECINSTSVMFRSTLPER 60

Query: 61  AMYPQSYTFDRVFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGIT 120
           +++P SYTFDRVFG DSTTKQVYEEGA+EVVLSVVNGINSTIFAYGQTSSGKT+TMNG+T
Sbjct: 61  SLFPHSYTFDRVFGIDSTTKQVYEEGAREVVLSVVNGINSTIFAYGQTSSGKTFTMNGVT 120

Query: 121 EYSVADIYNYMETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLT 180
           ++SVADIY+Y+E+HQ+RE+VLKFSAIEIYNEAV+DLLS ENVPLRLLDDPEKGTVVEKLT
Sbjct: 121 QHSVADIYSYIESHQDREFVLKFSAIEIYNEAVKDLLSLENVPLRLLDDPEKGTVVEKLT 180

Query: 181 EETLKDRNHLQELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLT 240
           EE LKDRNHLQEL+SFCEVQRKIGET+LNE SSRSHQILRLTIESSARKFKKSES S+LT
Sbjct: 181 EEILKDRNHLQELISFCEVQRKIGETSLNETSSRSHQILRLTIESSARKFKKSESSSTLT 240

Query: 241 ATVNFVDLAGSERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLT 300
           ATVNFVDLAGSERASQT S GTRLKEGCHINRSLL+LGTVIRKLSKGR GH+PYRDSKLT
Sbjct: 241 ATVNFVDLAGSERASQTNSGGTRLKEGCHINRSLLTLGTVIRKLSKGRNGHIPYRDSKLT 300

Query: 301 RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ 360
           RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ
Sbjct: 301 RILQNSLGGNGRTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQ 360

Query: 361 LQKELARLESEMKNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLL 420
           LQKELARLESEMKN+KPLP+KGDSNSLLKEKE++IEQMDKEIKELTRQRDLAQYRIENLL
Sbjct: 361 LQKELARLESEMKNLKPLPLKGDSNSLLKEKELVIEQMDKEIKELTRQRDLAQYRIENLL 420

Query: 421 HSVGEDRIFKRSESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDD--- 480
           HSVGEDRIFK SESTV                         T+PDLV+LDLDLRSDD   
Sbjct: 421 HSVGEDRIFKLSESTVH------------------------TIPDLVDLDLDLRSDDSSF 480

Query: 481 -----SSLKTFDTFNGQGQEENSPHKVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEV 540
                SSLKTFDTF    QEENSPHK++PLF+ +H+DDFLLDSSTPELAGP PY +WEE+
Sbjct: 481 KAFDESSLKTFDTFT--AQEENSPHKIDPLFTMNHEDDFLLDSSTPELAGPDPYQDWEEI 540

Query: 541 AQRVRANSEDSYKDVQCIELEESNQTPNENVELALARLEDNEGQMISTSRTNQVTSPQRK 600
           A+RV ANSED  KDVQCIELEE  +  NEN +L LA  EDNEGQMIS+   N  T PQR+
Sbjct: 541 AERVHANSEDGCKDVQCIELEELKELVNENGDLTLATWEDNEGQMISSFDINPETYPQRR 600

Query: 601 NKEIVTINKDYVSGGFMPNTAEPKRTLNCIVNFYPAEQSFSSIEAAKDRFQNLKLTRSKS 660
           NKEI+ I+K +   G +P  +E  +TLNCIVN YP+EQSF+SIEAAK RFQNLKL RSKS
Sbjct: 601 NKEIIPISKGHTYDGLIPKASEIGKTLNCIVNLYPSEQSFNSIEAAKTRFQNLKLERSKS 660

Query: 661 CLTVLMELPPSTWVEKPETDKKAQHNGSEINFSGLAEGSRRKRGLSCG--------NLET 720
           CLTVLM +PPST +EK + DKK +  GS++NFSG AEGSRR+RGLSCG        NL+T
Sbjct: 661 CLTVLMTIPPSTLIEKVKEDKKIRTVGSDVNFSGKAEGSRRRRGLSCGNLGANLEANLDT 720

Query: 721 EESQSVCNRPSEDKALEIIEEDDDDNTSVLNFATGKKGKSKNRLRKRSTHGSRLGRTPKR 780
           ++SQSVC+R S+ K L+IIEEDDDDNTSVLNFATGK+GKSKNR++KRS  GSRLG   K+
Sbjct: 721 KDSQSVCSRCSDTKTLQIIEEDDDDNTSVLNFATGKRGKSKNRMKKRS--GSRLGSLSKK 780

Query: 781 EEAKETTQDVGLEEEQDFQSHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDP 840
           EE  E T +V  +EE++ Q+HS+W+LEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDP
Sbjct: 781 EEPIEATLEVQTQEEKELQAHSEWILEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDP 840

Query: 841 SDAVYMEVELRRLFFIREAMSRSTNGFGRNDAITQASSLKTLNREREMLVKRIKKKFSVK 900
           +DAVYMEVELRRLFFIREA+SRS NG GR DA+TQ SSLK LNRER+ML +R+KKKF+VK
Sbjct: 841 TDAVYMEVELRRLFFIREAISRSVNGSGRGDAVTQTSSLKALNRERDMLARRMKKKFTVK 900

Query: 901 ERDTLYKKWGIDLKTKQRSIQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVSREMF 960
           ERD LY KWGIDLKTKQRSIQ+A+ LW+RTKDFDHI+ESAALVAKL+GFVEP+QVSREMF
Sbjct: 901 ERDALYVKWGIDLKTKQRSIQVARMLWSRTKDFDHINESAALVAKLIGFVEPNQVSREMF 956

Query: 961 GLSFSLQSLDQRSFPWKRNMSLPF 969
           GLS SLQSLD RSF WKRNMSLPF
Sbjct: 961 GLSISLQSLDHRSFSWKRNMSLPF 956

BLAST of CmaCh12G004270 vs. NCBI nr
Match: gi|720049503|ref|XP_010271468.1| (PREDICTED: kinesin-like protein NACK2 [Nelumbo nucifera])

HSP 1 Score: 883.2 bits (2281), Expect = 4.0e-253
Identity = 518/978 (52.97%), Postives = 673/978 (68.81%), Query Frame = 1

Query: 18  EETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDRAMYPQSYTFDRVFGCDS 77
           EE I VS+RLRPLN KE+ +N+ SDW C+N+N+IIFR++LP+R+MYP +YTFDRVF CD 
Sbjct: 22  EEKIFVSVRLRPLNAKEIARNEVSDWECINDNTIIFRNSLPERSMYPTAYTFDRVFRCDC 81

Query: 78  TTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYMETHQER 137
           +TKQVYEEGAKEV LSVV+GINS+IFAYGQTSSGKT+TM+GITEY+VADIY+Y++ H+ER
Sbjct: 82  STKQVYEEGAKEVALSVVSGINSSIFAYGQTSSGKTHTMSGITEYTVADIYDYIQRHEER 141

Query: 138 EYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHLQELLSFC 197
            +VLKFSA+EIYNEAVRDLLS ++ PLRLLDDPE+GTVVEKLTEE L+D NHL+ELLS C
Sbjct: 142 AFVLKFSAMEIYNEAVRDLLSVDSTPLRLLDDPERGTVVEKLTEEILRDWNHLKELLSIC 201

Query: 198 EVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGSERASQT 257
           E QRKIGET+LNE SSRSHQILRLTIESSAR+F   ++ S+L A+VNFVDLAGSERASQ 
Sbjct: 202 EAQRKIGETSLNETSSRSHQILRLTIESSAREFLGKDNSSTLAASVNFVDLAGSERASQA 261

Query: 258 MSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNGRTAIIC 317
           +SAGTRLKEGCHINRSLL+LGTVIRKLSKGR GH+PYRDSKLTRILQ SLGGN RTAIIC
Sbjct: 262 LSAGTRLKEGCHINRSLLTLGTVIRKLSKGRNGHIPYRDSKLTRILQPSLGGNARTAIIC 321

Query: 318 TMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESEMKNIKP 377
           TM PARSH+EQSRNTLLFA+CAKEV+TNA VN+V+SDKALVK LQ+ELARLESE++   P
Sbjct: 322 TMCPARSHLEQSRNTLLFASCAKEVATNAQVNIVMSDKALVKHLQRELARLESELRGPGP 381

Query: 378 LPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIFKRSESTVQ 437
                D ++LLKEK++ IE+M++EIKELT+QRDLAQ R+E+LL  VG+DR  +  E+   
Sbjct: 382 TSATCDFSALLKEKDLQIEKMEREIKELTQQRDLAQSRLEDLLRVVGDDRASRLWEA--- 441

Query: 438 IVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLKTFDTFNGQGQEENSPH 497
           +  ++  +  +    E+ S +V            D R  D  +  F T     ++ N+  
Sbjct: 442 LDHQSKFQVQNAWEDESDSSSV-----------ADPRCSDVGVAGFGTSQYSDRQSNTNS 501

Query: 498 KVNPLFSTSHDDD-FLLDSSTPELA-------GPYPYNNWEEVAQRVRANSEDSYKDVQC 557
            V       +  D FL D ++P+L+       GP P+ NWE +AQ    ++ED  K+V+C
Sbjct: 502 NVRYRHLPGNPQDRFLSDYTSPQLSNGSSEFVGPGPFQNWEGIAQETGEDNEDLCKEVRC 561

Query: 558 IELEESNQTPNENVELALARLEDNEGQMISTSRTNQ-------VTSPQRKNKEIVTINKD 617
           IE+E S  + N N++  +   E+NE  +  T   ++        + P + + E+  IN D
Sbjct: 562 IEMEAS--STNRNLKSNVLPPEENEELLPLTMNESRDAMEQELASFPPKGDGELRHINTD 621

Query: 618 YVSGGFMPNTAEPKRTLNCIVNFYPAEQS--FSSIEAAKDRFQNLKLTRSKSCLTVLMEL 677
           +            ++T+ C+VN Y  E S    S+E +  R  +L+LTRS+SC   LM  
Sbjct: 622 FTYDALEHKLHGMQKTIACLVNPYTDEPSPWPPSVELSSSR--SLRLTRSQSCRATLMTS 681

Query: 678 PPSTWVEKPETDKKAQHNGSEINFSGLAEGSRRK----------RGLSCGNLETEESQSV 737
             S W +   T      +G E +F G   G ++K          + LS  N +  E  SV
Sbjct: 682 SSSPWFDNQNTPP----SGFEKDFPGRPCGFQKKPSALNFSANIQRLSRKNSQNSEG-SV 741

Query: 738 CNRPSEDKALEIIEEDDDDNTSVLNFATGKKGKSKNRLRKRSTHGSRLGRTPKREEAKET 797
           C    E KA  I    +DD TS+  F TG K  +K +  K+   G  L   PK +++  T
Sbjct: 742 CT--DELKAQNIKTSAEDDITSIHTFVTGLKEMAKLQYEKQIDDG--LETEPKADKSGTT 801

Query: 798 TQDVGLEEEQD-FQSHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDPSDAVY 857
            ++VG++  QD   S   W LEF+ QQR+IIELW  CNV L+HR+YF +LF GDP+D++Y
Sbjct: 802 VKNVGVDPMQDPSDSPLSWPLEFERQQREIIELWHTCNVSLIHRTYFLLLFGGDPADSIY 861

Query: 858 MEVELRRLFFIREAMSR---STNGFGRNDAITQASSLKTLNREREMLVKRIKKKFSVKER 917
           M+VELRRL FIR+  S+   + +    + A+T ASS++ L REREML KR+ K+FS  ER
Sbjct: 862 MKVELRRLSFIRDTFSQGNLAKHAMIDDWALTPASSMRALRREREMLSKRMPKRFSEGER 921

Query: 918 DTLYKKWGIDLKTKQRSIQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVSREMFGL 965
           + LYKKWGI L TKQR +QLA++LWT TKD DH+ ESA +VAKL+GF EP Q  +EMFGL
Sbjct: 922 EQLYKKWGIGLDTKQRRLQLARRLWTDTKDMDHVMESATIVAKLIGFQEPGQALKEMFGL 972

BLAST of CmaCh12G004270 vs. NCBI nr
Match: gi|359492713|ref|XP_002280678.2| (PREDICTED: kinesin-like protein NACK1 [Vitis vinifera])

HSP 1 Score: 841.3 bits (2172), Expect = 1.7e-240
Identity = 483/962 (50.21%), Postives = 651/962 (67.67%), Query Frame = 1

Query: 14  SNGLEETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTL--PDRAMYPQSYTFDR 73
           + G EE I VS+RLRPL+ KE ++   SDW C+N N+II++++L  P+R+ +P +YTFDR
Sbjct: 14  AKGQEEKILVSVRLRPLDVKENSRYHVSDWECINVNTIIYKNSLSLPERSQFPTAYTFDR 73

Query: 74  VFGCDSTTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYM 133
           VFG + +T++VY+EGAKEV LSVVNGINS+IFAYGQTSSGKTYTM GITEY+V+DIY+Y+
Sbjct: 74  VFGQNCSTREVYDEGAKEVALSVVNGINSSIFAYGQTSSGKTYTMTGITEYAVSDIYDYV 133

Query: 134 ETHQEREYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHLQ 193
           E H++RE+ LKFSA+EIYNEA+RDLLS ++ PLRLLDDPE+GTVV+KLTEETL+DRNHLQ
Sbjct: 134 ERHRDREFKLKFSAMEIYNEALRDLLSSDSAPLRLLDDPERGTVVDKLTEETLRDRNHLQ 193

Query: 194 ELLSFCEVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGS 253
           ELLS CE QR+IGET LNE SSRSHQILRLTIESSA KF  +E+ SSL ATV+FVDLAGS
Sbjct: 194 ELLSICEAQRQIGETALNETSSRSHQILRLTIESSAHKFVGAENSSSLAATVSFVDLAGS 253

Query: 254 ERASQTMSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNG 313
           ERASQT+S GTRLKEGCHINRSLL+LGTVIRKLSKGR  H+PYRDSKLTRILQNSLGGN 
Sbjct: 254 ERASQTLSEGTRLKEGCHINRSLLTLGTVIRKLSKGRNVHIPYRDSKLTRILQNSLGGNA 313

Query: 314 RTAIICTMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESE 373
           RTAIICTMSPARSH+EQSRNTLLFA+CAKEVSTNAHVNVV+SDK LVK LQ+E+ARLESE
Sbjct: 314 RTAIICTMSPARSHIEQSRNTLLFASCAKEVSTNAHVNVVMSDKILVKHLQREMARLESE 373

Query: 374 MKNIKPLPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIFKR 433
           +++++      DS +LLKEKE+LIE+MDKEIK+LT+QRDLA  +IE+LL S+GED+  K+
Sbjct: 374 LRSLELNHAANDSTALLKEKELLIEKMDKEIKDLTQQRDLAHSQIEDLLKSIGEDQ-SKQ 433

Query: 434 SESTVQIVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLKTFDTFNGQGQ 493
           S  + QI    +  T S+    + S  +P +    ++LDL   S        +  N +G 
Sbjct: 434 SMESDQISEHQVQNTWSDEPSASESSDMPNS--HCLDLDLTTCSSSQYSDHDNGLNSRGD 493

Query: 494 EENSPHKVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEVAQRVRANSEDSYKDVQCIE 553
               P      F  S D   +L ++TP   GP P   WE+  Q +  N+ED         
Sbjct: 494 SLQLPENSENHF-PSDDASSILSTNTPIFVGPNPCQGWEKTIQGLDRNTED--------- 553

Query: 554 LEESNQTPNENVELALARLEDNEGQMIST--SRTNQVTSPQRKNKEIVTINKDYVSGGFM 613
                       + +L   E+ +G++  T    T+ ++S                 G   
Sbjct: 554 ------------DTSLPCPEEKDGKLALTVAGDTDAISS----------------HGSLE 613

Query: 614 PNTAEPKRTLNCIVNFYPAEQSFSSIEAAKDRFQNLKLTRSKSCLTVLMELPPSTWVEKP 673
               + K+T+  + + YP E S    EA K   ++L+L RS+SC +V+M +  S   ++ 
Sbjct: 614 QKIQDMKKTIESLFSMYPLEPSLCFTEADKSSSRSLRLNRSRSCRSVIMTI-QSPLFDEA 673

Query: 674 ETDKKAQHNGSEINFSGLAEG-----SRRKRGLSCGNLETEESQ-SVCNRPSEDKALEII 733
           E  +    NG + +F G  EG     +  + G        ++S+ SV +   ++KA  + 
Sbjct: 674 EQGESILPNGLDEDFPGRPEGFLPKLAEMEFGDGMKKFSRQDSRTSVRSVSMDEKAQNVK 733

Query: 734 EEDDDDNTSVLNFATGKKGKSKNRLRKRSTHGSRLGRTPKREEAKETTQDVGLEEEQDFQ 793
              + D  S  +F       ++ +        + +  TP  ++     + V  + +Q+  
Sbjct: 734 TSGEWDTNSAHDFVAKLNEMAEVQSAMELGDDTVMETTPDADDTAGKNK-VDRDTKQNAS 793

Query: 794 SHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDPSDAVYMEVELRRLFFIREA 853
               W LEF+ QQR+II LWD+CNVPLVHR+YFF+LFKG+  D+VYMEVELRRL+F++E+
Sbjct: 794 KSLSWALEFKRQQREIIALWDSCNVPLVHRTYFFLLFKGNKLDSVYMEVELRRLYFLKES 853

Query: 854 MSRSTNGFGRNDAITQASSLKTLNREREMLVKRIKKKFSVKERDTLYKKWGIDLKTKQRS 913
            S  +     +  +T ASS + LNREREML+K+++K+FS KE +T+Y+KWGIDL +KQR 
Sbjct: 854 FSHGSGAVKDDQPLTLASSKRALNREREMLIKQVQKRFSRKEMETIYQKWGIDLDSKQRK 913

Query: 914 IQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVSREMFGLSFSLQSLDQRSFPWKRN 966
           +QL +++W+  +D +HI ESAALVAKL+GF+ PS+  +E+FGLSFS + + +RS+ W+ N
Sbjct: 914 LQLVRRIWSDIRDMNHIRESAALVAKLVGFIVPSEAPQEIFGLSFSPKPMTRRSYSWRSN 932

BLAST of CmaCh12G004270 vs. NCBI nr
Match: gi|223551183|gb|EEF52669.1| (ATP binding protein, putative [Ricinus communis])

HSP 1 Score: 830.5 bits (2144), Expect = 3.1e-237
Identity = 488/973 (50.15%), Postives = 650/973 (66.80%), Query Frame = 1

Query: 18  EETIRVSIRLRPLNEKELTKNDSSDWACLNNNSIIFRSTLPDRAMYPQSYTFDRVFGCDS 77
           EE I VS+R+RPLNE+E+ +ND  DW C+NNN+IIF+S +PDR+M P +YTFDRVFG + 
Sbjct: 25  EEKIFVSVRMRPLNEREVARNDICDWECINNNTIIFKSNMPDRSMVPTAYTFDRVFGSEC 84

Query: 78  TTKQVYEEGAKEVVLSVVNGINSTIFAYGQTSSGKTYTMNGITEYSVADIYNYMETHQER 137
           +TKQVYEEGAKE+ L+ V+GINS+IFAYGQTSSGKTYTM+G+TEY+VADIY YM  H+ER
Sbjct: 85  STKQVYEEGAKEIALAAVSGINSSIFAYGQTSSGKTYTMDGVTEYAVADIYEYMNKHKER 144

Query: 138 EYVLKFSAIEIYNEAVRDLLSFENVPLRLLDDPEKGTVVEKLTEETLKDRNHLQELLSFC 197
           E+VLKFSA+EIYNEAVRDLLS ++ PLR+LDDPEKGTVVE+L EETL D NHLQELL+ C
Sbjct: 145 EFVLKFSAMEIYNEAVRDLLSSDSTPLRVLDDPEKGTVVERLIEETLIDWNHLQELLTIC 204

Query: 198 EVQRKIGETTLNEASSRSHQILRLTIESSARKFKKSESGSSLTATVNFVDLAGSERASQT 257
           E QR+IGET++NE SSRSHQ++RLTIESSAR++    + S L +TV+FVDLAGSERASQT
Sbjct: 205 EAQRQIGETSMNENSSRSHQVIRLTIESSAREYSGVGNSSILISTVHFVDLAGSERASQT 264

Query: 258 MSAGTRLKEGCHINRSLLSLGTVIRKLSKGRTGHVPYRDSKLTRILQNSLGGNGRTAIIC 317
           ++ G RLKEG HINRSLL+LGTVIRKLSKG+ GH+PYRDSKLTRILQNSLGGN RTA+IC
Sbjct: 265 LAGGARLKEGSHINRSLLTLGTVIRKLSKGKNGHIPYRDSKLTRILQNSLGGNARTAMIC 324

Query: 318 TMSPARSHVEQSRNTLLFATCAKEVSTNAHVNVVVSDKALVKQLQKELARLESEMKNIKP 377
           T+SP+RSHVEQSRNTLLFA+CA EV+TNA VNVV+SDKALVKQL+KELA+LES +K+++ 
Sbjct: 325 TISPSRSHVEQSRNTLLFASCANEVATNAQVNVVMSDKALVKQLRKELAKLESRLKSMES 384

Query: 378 LPVKGDSNSLLKEKEILIEQMDKEIKELTRQRDLAQYRIENLLHSVGEDRIFKRSESTVQ 437
           + V GD+ +LL+EKE+LIE+MDKEIKELT QRDLAQ R+++LL  VG++R+ +  E++  
Sbjct: 385 ISVTGDTAALLREKELLIEKMDKEIKELTWQRDLAQSRVDSLLREVGDNRLSRVGENSA- 444

Query: 438 IVPETMSETMSETVPETMSETVPATVPDLVELDLDLRSDDSSLKTFD-----TFNGQGQE 497
                 SE+     P  +      T               +++K FD     T   Q Q+
Sbjct: 445 ------SESSEGITPLGLDAGFART---------------NTVKDFDDPSVLTPTRQIQQ 504

Query: 498 ENSPHKVNPLFSTSHDDDFLLDSSTPELAGPYPYNNWEEVAQRVRANSEDSYKDVQCIEL 557
              P           +D FLL++STP+ + P P+  WE  +Q    N+ED  K+V+CIE 
Sbjct: 505 IPDP-----------EDGFLLNNSTPKFSEPDPFRFWESTSQE---NNEDICKEVRCIET 564

Query: 558 EESNQTPNENVELALARLEDNEGQMISTSRTNQVTSP----QRKNKEIVTINKDYVSGGF 617
           +E++        + L+  +  E ++ +   T++   P    Q ++KE  + N  Y S   
Sbjct: 565 DEASVISKAEDGVLLSSSDRQEKEVATAEETDEGALPSMAMQEEDKES-SHNDSYNSYHA 624

Query: 618 MPNTAEP-KRTLNCIVNFYPAEQSFSSIEAAKDRFQNLKLTRSKSCLTVLMELPPSTWVE 677
           +    +    T++ +  F P E   SS +A       L  +RSKS  +V+M +P + W E
Sbjct: 625 LKQKIQDLHETISLLQQFPPNEAGASSSKA-------LTWSRSKSRRSVVMTIPSALWYE 684

Query: 678 KP-----------ETDKKAQHNGSEINFSGLAEGSRRKRGLSCGNLETEESQSVCNRPSE 737
           K            E D   +  G+E   + L      K G   G      + S C    E
Sbjct: 685 KEEENENILPASFEEDNLERRGGTEQKLAELEPDD--KTGKISGKYSRNSTSSAC---IE 744

Query: 738 DKALEIIEEDDDDNTSVLNFATG----KKGKSKNRLRKRSTHGSRLGRTPKREEAKETTQ 797
           ++ ++ I  D DD T+VL+F  G     K +S+ ++ + ST  + L R   R  +     
Sbjct: 745 EETIKEINIDVDDTTTVLDFVAGVNTIAKPQSEEQI-EASTRTADLWRDAGRGNSAR--- 804

Query: 798 DVGLEEEQDFQSHSDWLLEFQGQQRDIIELWDACNVPLVHRSYFFILFKGDPSDAVYMEV 857
                     Q+ ++ +L+F+  +R IIELW  CNVPLVHRSYFF++FKGDPSD VY+EV
Sbjct: 805 ----------QNRANSILKFERYRRKIIELWARCNVPLVHRSYFFLIFKGDPSDNVYLEV 864

Query: 858 ELRRLFFIREAMSRSTNGFGRNDAITQASSLKTLNREREMLVKRIKKKFSVKERDTLYKK 917
           ELRRL+F+++  +R TN       ++  SSLK+LNRERE L ++++KKF+ +ER  LY +
Sbjct: 865 ELRRLYFLKDTSARGTNTLIDTQIVSLNSSLKSLNREREHLARQLQKKFTKRERVELYVR 924

Query: 918 WGIDLKTKQRSIQLAKKLWTRTKDFDHIHESAALVAKLLGFVEPSQVSREMFGLSFSLQS 966
           WGIDL TKQRS+QL ++LWT TKD  H+ ES+ LVAKL+GFVEP    +EMFGLSF    
Sbjct: 925 WGIDLDTKQRSLQLIRRLWTDTKDLKHMRESSVLVAKLIGFVEPRYAPKEMFGLSFLTPL 934